Carg25817 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25817
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	sphinganine C4-monooxygenase 1-like
Location	Carg_Chr05: 5990921 .. 5992998 (-)
RNA-Seq Expression	Carg25817
Synteny	Carg25817

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTCATCATCTCCGATGATTTGTTGGGTACTTTTGTCCCCATTTTCGTTTATTGGTTATATTCTGGCATATATGTGCTTCTCGGTTTCTTCCACAATTATCGATTGCACTCCAAGACAGAGGAGGATGAGAAGAACTTAGTTTCAAAATCCACTGTGGTTCGGGGCGTTCTCTTTCAGCAATTCGTTCAGGCTATCGTCGCCATCATCCTCTTTAAGGTTAGCTTCTTGTTTTCTTCAGTTTCTTTACCACTATTGCAATCATAACCATCGTCCTTGTCGGTTTTGGCATTGTTATTATAATTTAGTTTATTTCTGATCCTAATTATTGGCGGAACCTGCCCTACAAAATTCCAACTGAGATTTATTTTTCTCGTACACTTGGCTGAAACCTTGATGTAATCTTCCTGATTTTTTCTTAGTATAGCAGCTGTGTGTGGAGCACGTTGATTACAGGTTTACCAGCCTAGATATAGTTCGTTGTTTGTGGACCTTTTGAGTTCGACAAAATGAAAATAGTAACTCCTTTTCTCTTTACATGTCTTTTGAATTTTGATCCAAACAAGAACAGACTAGTTACCAATTGTCAATTTCGGTTTCTTATAATTGTAACTATTGTATATTTATTTCAAAGATGATTCGTTGCTTTAAAAAAAAAAAAAAAATCACATCAAAATAAGTTAGATGGCAATTGGGAAAATTTTAATTTGGTTGAAGAATAAGAGGAGTTAATGATTCAAGTTAGGAAGTTGAAAGAATTTTTTTTTTTTTTTGCAGTTTTTAGTTTCTCCAGTAAAGCTATATTAATTGATGCAAGGCTGTTAACTTTTATCTGTAAGTGTTTTTTATATTGAACACAACAAGAACAGCTTTTCAAGATAGATATGAACAAAATTGTAGGCTATAACGCCTTTTTCATTGTTAAATTCCCCCTATGAGATGATCGTTTGAACTAGAAAGTTATTGTTTAAAAAAACAACACGATATTTCTGCTTTGTTTGAAAATTAAATTCCACTTCCTTCAACTATAATGTAATCCTTTGGATTAGGTTTCATGTTTATTCACAATTTTGTGCCACATTCAAATCCTTCTGACTCCCAGAATCTGTGTTATTGTCTTTCAAATTTAAGTTCAAATTTTTTGGGATAGCATCAATCCCCTGTTGTGTTATGCTTCGGCACTCGGTAGGAAGTCACCAACTCAGACAAACAAAATATTTGGAACTTCCATTTAGTTTTGTCTCATGTGTTGCATGAATTTGCTACACTACTCTTGCTATTGCTAAATCAGATGTAGACCGGTAGTTTAGTGGATGTTCATGTGAAATAGACATCCTATTTAAATGAATTATTTGGGTTTGGCCCCGTGGATAAGAGTATTTATGAGCTCAAACTAAGAAGCCACGGTATTCAGGACATCCTTAGAAACTATGGATTCGTAATATTCCATGACTGATATCATGTGGAGTGTCTGCTCTGCACTTTCATACTGGTTTGTGTCCGAATTTATAATTGCATCGTGTTATAACGTTCAGGTGACTGGAAATGAAGATGGGAGCAGTGCAGTTCCAAAATCCTGGCCAATGGTTGCTTTGCAATTTGTAACCGCGATGTTCGTTCTGGACACGTGGCAGTACTTCGCACACAGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCATCACCATAGGCTCGTTGTTCCATATGCATTTGGGGCATTGTATAATCATCCTGTGGAAGGTCTACTGCTTGACATGGTTGGTGGGGTGTTGTCCTTTCTAGCATCTGGTATGACTCCTAGAGTCTCCATCTTCTTCTTTTCCTTCGCCACTATTAAGACTGTAGACGATCACTGTGGATTATGGCTGCCTGGTAACCTCTTCCACCTCTTATTTCGCAACAATACTGCATATCACGACCTCCACCATCAGCTATATGGCAACAAGGTTAACTATTCACAACCCTTTTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCCTGGAGAAGAGAGCAGGCGGCGGCTTTGAAGCACGCCCAAAGATTGAATAA

mRNA sequence

ATGGAATTCATCATCTCCGATGATTTGTTGGGTACTTTTGTCCCCATTTTCGTTTATTGGTTATATTCTGGCATATATGTGCTTCTCGGTTTCTTCCACAATTATCGATTGCACTCCAAGACAGAGGAGGATGAGAAGAACTTAGTTTCAAAATCCACTGTGGTTCGGGGCGTTCTCTTTCAGCAATTCGTTCAGGCTATCGTCGCCATCATCCTCTTTAAGGTGACTGGAAATGAAGATGGGAGCAGTGCAGTTCCAAAATCCTGGCCAATGGTTGCTTTGCAATTTGTAACCGCGATGTTCGTTCTGGACACGTGGCAGTACTTCGCACACAGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCATCACCATAGGCTCGTTGTTCCATATGCATTTGGGGCATTGTATAATCATCCTGTGGAAGGTCTACTGCTTGACATGGTTGGTGGGGTGTTGTCCTTTCTAGCATCTGGTATGACTCCTAGAGTCTCCATCTTCTTCTTTTCCTTCGCCACTATTAAGACTGTAGACGATCACTGTGGATTATGGCTGCCTGGTAACCTCTTCCACCTCTTATTTCGCAACAATACTGCATATCACGACCTCCACCATCAGCTATATGGCAACAAGGTTAACTATTCACAACCCTTTTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCCTGGAGAAGAGAGCAGGCGGCGGCTTTGAAGCACGCCCAAAGATTGAATAA

Coding sequence (CDS)

ATGGAATTCATCATCTCCGATGATTTGTTGGGTACTTTTGTCCCCATTTTCGTTTATTGGTTATATTCTGGCATATATGTGCTTCTCGGTTTCTTCCACAATTATCGATTGCACTCCAAGACAGAGGAGGATGAGAAGAACTTAGTTTCAAAATCCACTGTGGTTCGGGGCGTTCTCTTTCAGCAATTCGTTCAGGCTATCGTCGCCATCATCCTCTTTAAGGTGACTGGAAATGAAGATGGGAGCAGTGCAGTTCCAAAATCCTGGCCAATGGTTGCTTTGCAATTTGTAACCGCGATGTTCGTTCTGGACACGTGGCAGTACTTCGCACACAGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCATCACCATAGGCTCGTTGTTCCATATGCATTTGGGGCATTGTATAATCATCCTGTGGAAGGTCTACTGCTTGACATGGTTGGTGGGGTGTTGTCCTTTCTAGCATCTGGTATGACTCCTAGAGTCTCCATCTTCTTCTTTTCCTTCGCCACTATTAAGACTGTAGACGATCACTGTGGATTATGGCTGCCTGGTAACCTCTTCCACCTCTTATTTCGCAACAATACTGCATATCACGACCTCCACCATCAGCTATATGGCAACAAGGTTAACTATTCACAACCCTTTTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCCTGGAGAAGAGAGCAGGCGGCGGCTTTGAAGCACGCCCAAAGATTGAATAA

Protein sequence

MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLFQQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFLYRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKTVDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEKRAGGGFEARPKIE

Homology

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BLAST of Carg25817 vs. NCBI nr
Match: XP_022946507.1 (sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >XP_022946508.1 sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >KAG6598981.1 Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029949.1 Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 522.3 bits (1344), Expect = 2.3e-144
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

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BLAST of Carg25817 vs. NCBI nr
Match: XP_023546641.1 (sphinganine C4-monooxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.8 bits (1335), Expect = 2.5e-143
Identity = 252/253 (99.60%), Postives = 252/253 (99.60%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFF NYRLHSKTEEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFDNYRLHSKTEEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

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BLAST of Carg25817 vs. NCBI nr
Match: XP_022972857.1 (sphinganine C4-monooxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 518.1 bits (1333), Expect = 4.3e-143
Identity = 250/253 (98.81%), Postives = 252/253 (99.60%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEFIISDDLLGTFVPIFVYWLYSG+YVLLGFF NYRLHSKTEEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFIISDDLLGTFVPIFVYWLYSGVYVLLGFFDNYRLHSKTEEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQF+QAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

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BLAST of Carg25817 vs. NCBI nr
Match: XP_008441706.1 (PREDICTED: sphinganine C4-monooxygenase 1-like [Cucumis melo] >KAA0032268.1 sphinganine C4-monooxygenase 1-like [Cucumis melo var. makuwa] >TYK17072.1 sphinganine C4-monooxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 492.3 bits (1266), Expect = 2.5e-135
Identity = 234/253 (92.49%), Postives = 245/253 (96.84%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEF ISDDLLGTFVPIFVYW YSG+Y+LLGFF NYRLHSKT+EDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQF+QAIVAIILFKVTGN+DGS+AVPKSWPMV LQF+TAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHS HHRLVVPYAFGALYNHPVEGLLLD VGG LSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHD+HHQLYG+K N+SQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of Carg25817 vs. NCBI nr
Match: XP_038889456.1 (sphinganine C4-monooxygenase 1-like [Benincasa hispida])

HSP 1 Score: 488.0 bits (1255), Expect = 4.8e-134
Identity = 232/253 (91.70%), Postives = 243/253 (96.05%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEF ISDDLLGTFVPIFVYW YSGIYVLLGFF NYRLHSKT+EDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQ +QAIVAIILFKVTGN+DGS+AVPKSWPMV LQF+TAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQIIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           Y+HIHS HHRLVVPYAFGALYNHPVEGLLLD VGG LSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YKHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH++F NNTAYHD+HHQLYG+K N+SQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHIIFGNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of Carg25817 vs. ExPASy Swiss-Prot
Match: Q8VYI1 (Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 6.6e-110
Identity = 191/254 (75.20%), Postives = 216/254 (85.04%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGT  PI VYWLYSGIYV L    +YRLHSK EE+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFVQAIVAIILFKVTGNE-DGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKF 120
           QQ VQA+VAI+LF VTG++ +   A   S+ ++A QFVTAM VLDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIK 180
           LY+HIHS HHRL+VPYA+GALYNHPVEGLLLD +GG LSFL SGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG K N+SQPFFV WDRILGTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

[Close]

BLAST of Carg25817 vs. ExPASy Swiss-Prot
Match: Q9AST3 (Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-106
Identity = 180/251 (71.71%), Postives = 213/251 (84.86%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           M F+ISD+ LGTFVPI VYW+YSG+Y+ LG    YRLHSK +EDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFVQAIVAIILFKVTGNE-DGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKF 120
           QQ +QAI+++ILFK+TG++ D ++    S  ++A QF+ AM V+DTWQYF HRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIK 180
           LY+HIHS HHRL+VPY++GALYNHP+EGLLLD +GG LSFL SGM+PR +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGN FH+ F NN+AYHD+HHQLYG K N+SQPFFV WDRILGTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRAGGGFEARP 251
           KRA GGFE RP
Sbjct: 242 KRANGGFETRP 252

[Close]

BLAST of Carg25817 vs. ExPASy Swiss-Prot
Match: B8B4W4 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39946 GN=GL1-9 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 4.3e-93
Identity = 169/254 (66.54%), Postives = 199/254 (78.35%), Query Frame = 0


Query: 2   EFIISDDLLGTFVPIFVYWLYSGIYVLLGF---FHNYRLHSKTEEDEKNLVSKSTVVRGV 61
           E  +SD+ +GTF PI +YW+Y+G Y L+        YRLH++ EE+EKNLV+   VVRGV
Sbjct: 5   EGYVSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGV 64

Query: 62  LFQQFVQAIVAIILFKVTGNEDGSSAVPKSWPMV--ALQFVTAMFVLDTWQYFAHRYMHH 121
           L QQ VQAIVA+ILF VT +   SSAV    P++  A QF+ AM V+D+WQYF HRYMH 
Sbjct: 65  LLQQLVQAIVAMILFMVTSD---SSAVVVQPPIIIQAFQFLVAMLVMDSWQYFVHRYMHQ 124

Query: 122 NKFLYRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFA 181
           NKFLYRHIHS HHRL+VPYA GALYNHP+EGLLLD VGG +SFL SGMTPR S+FFF FA
Sbjct: 125 NKFLYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFA 184

Query: 182 TIKTVDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPY 241
            +KTVDDHCGLWLP N+F  LF+NNTAYHD+HHQL G+K NYSQPFF  WDRILGT+MPY
Sbjct: 185 VLKTVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPY 244

Query: 242 SLEKRAGGGFEARP 251
           +L +R  GGFEARP
Sbjct: 245 NLVRRKEGGFEARP 255

[Close]

BLAST of Carg25817 vs. ExPASy Swiss-Prot
Match: Q0D4G3 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-9 PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 4.3e-93
Identity = 169/254 (66.54%), Postives = 199/254 (78.35%), Query Frame = 0


Query: 2   EFIISDDLLGTFVPIFVYWLYSGIYVLLGF---FHNYRLHSKTEEDEKNLVSKSTVVRGV 61
           E  +SD+ +GTF PI +YW+Y+G Y L+        YRLH++ EE+EKNLV+   VVRGV
Sbjct: 5   EGYVSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGV 64

Query: 62  LFQQFVQAIVAIILFKVTGNEDGSSAVPKSWPMV--ALQFVTAMFVLDTWQYFAHRYMHH 121
           L QQ VQAIVA+ILF VT +   SSAV    P++  A QF+ AM V+D+WQYF HRYMH 
Sbjct: 65  LLQQLVQAIVAMILFMVTSD---SSAVVVQPPIIIQAFQFLVAMLVMDSWQYFVHRYMHQ 124

Query: 122 NKFLYRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFA 181
           NKFLYRHIHS HHRL+VPYA GALYNHP+EGLLLD VGG +SFL SGMTPR S+FFF FA
Sbjct: 125 NKFLYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFA 184

Query: 182 TIKTVDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPY 241
            +KTVDDHCGLWLP N+F  LF+NNTAYHD+HHQL G+K NYSQPFF  WDRILGT+MPY
Sbjct: 185 VLKTVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPY 244

Query: 242 SLEKRAGGGFEARP 251
           +L +R  GGFEARP
Sbjct: 245 NLVRRKEGGFEARP 255

[Close]

BLAST of Carg25817 vs. ExPASy Swiss-Prot
Match: O94298 (Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sur2 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.3e-54
Identity = 109/253 (43.08%), Postives = 149/253 (58.89%), Query Frame = 0


Query: 15  PIFVYWLYSGI-----YVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLFQQFVQAIVA 74
           P+ +YW+ S       Y+ L  F  YR+H   E   +N V +  VV+ VLFQQ  + +V 
Sbjct: 21  PVIIYWVASAFFGFLHYIELPVFEKYRIHPPEEIARRNRVPQMAVVKAVLFQQLCEVVVG 80

Query: 75  IILFKVTGNEDG------------------------SSAVPK---SWPMVALQFVTAMFV 134
           I L    G  +                         +   PK   ++ + A Q+  A F+
Sbjct: 81  IALAMFEGYPEPIDEAKQMLRYEAFFSKNLPALLQVAPFAPKLAYNFIVPAFQYFFAFFI 140

Query: 135 LDTWQYFAHRYMHHNKFLYRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLAS 194
           +D+WQYF HRY+H+NK LY  IH+HHHRL VPYA GALYNHP EGL+LD  G  +++LA+
Sbjct: 141 IDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDTFGAGVAYLAA 200

Query: 195 GMTPRVSIFFFSFATIKTVDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPF 236
           G++P+ ++ FF+ +T+KTVDDHCG   P +   + F NN  YHDLHHQ YG + N+SQPF
Sbjct: 201 GLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPYGFQKNFSQPF 260

[Close]

BLAST of Carg25817 vs. ExPASy TrEMBL
Match: A0A6J1G422 (sphinganine C4-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111450547 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.1e-144
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of Carg25817 vs. ExPASy TrEMBL
Match: A0A6J1I5Y6 (sphinganine C4-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471367 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.1e-143
Identity = 250/253 (98.81%), Postives = 252/253 (99.60%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEFIISDDLLGTFVPIFVYWLYSG+YVLLGFF NYRLHSKTEEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFIISDDLLGTFVPIFVYWLYSGVYVLLGFFDNYRLHSKTEEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQF+QAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of Carg25817 vs. ExPASy TrEMBL
Match: A0A5D3CZU2 (Sphinganine C4-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1327G00180 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.2e-135
Identity = 234/253 (92.49%), Postives = 245/253 (96.84%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEF ISDDLLGTFVPIFVYW YSG+Y+LLGFF NYRLHSKT+EDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQF+QAIVAIILFKVTGN+DGS+AVPKSWPMV LQF+TAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHS HHRLVVPYAFGALYNHPVEGLLLD VGG LSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHD+HHQLYG+K N+SQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of Carg25817 vs. ExPASy TrEMBL
Match: A0A1S3B426 (sphinganine C4-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103485785 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.2e-135
Identity = 234/253 (92.49%), Postives = 245/253 (96.84%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEF ISDDLLGTFVPIFVYW YSG+Y+LLGFF NYRLHSKT+EDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQF+QAIVAIILFKVTGN+DGS+AVPKSWPMV LQF+TAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YRHIHS HHRLVVPYAFGALYNHPVEGLLLD VGG LSFLASGMTPRVSIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHD+HHQLYG+K N+SQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of Carg25817 vs. ExPASy TrEMBL
Match: A0A6J1DY75 (sphinganine C4-monooxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111025684 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.3e-134
Identity = 232/253 (91.70%), Postives = 244/253 (96.44%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           MEF ISD+LLGTFVPIFVYWLYSGIYVLLGFF NYRLHSK +EDEKNLVSK+TVVRGVLF
Sbjct: 1   MEFTISDELLGTFVPIFVYWLYSGIYVLLGFFENYRLHSKKDEDEKNLVSKATVVRGVLF 60

Query: 61  QQFVQAIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFL 120
           QQF+QAIVAIILFKVTGN+DGSSAVPKSWPMV LQF+TAMFVLDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSSAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIKT 180
           YR+IHS HHRLVVPYAFGALYNHPVEGLLLD +GG LSFL SGMTPRVSIFFFSFATIKT
Sbjct: 121 YRYIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHD+HHQLYG+K N+SQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RAGGGFEARPKIE 254
           RAGGGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of Carg25817 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 398.3 bits (1022), Expect = 4.7e-111
Identity = 191/254 (75.20%), Postives = 216/254 (85.04%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGT  PI VYWLYSGIYV L    +YRLHSK EE+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFVQAIVAIILFKVTGNE-DGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKF 120
           QQ VQA+VAI+LF VTG++ +   A   S+ ++A QFVTAM VLDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIK 180
           LY+HIHS HHRL+VPYA+GALYNHPVEGLLLD +GG LSFL SGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG K N+SQPFFV WDRILGTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

[Close]

BLAST of Carg25817 vs. TAIR 10
Match: AT1G14290.1 (sphingoid base hydroxylase 2 )

HSP 1 Score: 387.5 bits (994), Expect = 8.2e-108
Identity = 180/251 (71.71%), Postives = 213/251 (84.86%), Query Frame = 0


Query: 1   MEFIISDDLLGTFVPIFVYWLYSGIYVLLGFFHNYRLHSKTEEDEKNLVSKSTVVRGVLF 60
           M F+ISD+ LGTFVPI VYW+YSG+Y+ LG    YRLHSK +EDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFVQAIVAIILFKVTGNE-DGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKF 120
           QQ +QAI+++ILFK+TG++ D ++    S  ++A QF+ AM V+DTWQYF HRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYRHIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSIFFFSFATIK 180
           LY+HIHS HHRL+VPY++GALYNHP+EGLLLD +GG LSFL SGM+PR +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHLLFRNNTAYHDLHHQLYGNKVNYSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGN FH+ F NN+AYHD+HHQLYG K N+SQPFFV WDRILGTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRAGGGFEARP 251
           KRA GGFE RP
Sbjct: 242 KRANGGFETRP 252

[Close]

BLAST of Carg25817 vs. TAIR 10
Match: AT2G29390.3 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 75.5 bits (184), Expect = 6.9e-14
Identity = 72/241 (29.88%), Postives = 109/241 (45.23%), Query Frame = 0


Query: 10  LGTFVPIFVYWLYSGI-YVLL---GFFHNYRLHSKTEEDEKNLVSKSTVVRGVLFQQFVQ 69
           +G+F+     +  SG+ Y+ L   GF  NY++ +K+   E        + R +L+   V 
Sbjct: 17  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 76

Query: 70  ---AIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G E  SS    SW +V+ Q +    + D   Y+ HR + H K+LY+
Sbjct: 77  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 136

Query: 130 HIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSI---FFFSFATIK 189
           ++HS HH    P+   + Y HP E L L    G  + +   +T    I    +     I+
Sbjct: 137 NVHSVHHEYATPFGLTSEYAHPAEILFL----GFATIVGPALTGPHLITLWLWMMLRVIE 196

Query: 190 TVDDHCGL---WLPGNLFHLLFRNNTAYHDLHHQ-LYGNKVNYSQPFFVTWDRILGTYMP 237
           TV+ HCG    W P N   L       +HD HH+ LY    NYS   FV  D I GT   
Sbjct: 197 TVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSST-FVYMDWIFGTDKG 244

[Close]

BLAST of Carg25817 vs. TAIR 10
Match: AT2G29390.2 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 75.5 bits (184), Expect = 6.9e-14
Identity = 72/241 (29.88%), Postives = 109/241 (45.23%), Query Frame = 0


Query: 10  LGTFVPIFVYWLYSGI-YVLL---GFFHNYRLHSKTEEDEKNLVSKSTVVRGVLFQQFVQ 69
           +G+F+     +  SG+ Y+ L   GF  NY++ +K+   E        + R +L+   V 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  ---AIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G E  SS    SW +V+ Q +    + D   Y+ HR + H K+LY+
Sbjct: 84  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 143

Query: 130 HIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSI---FFFSFATIK 189
           ++HS HH    P+   + Y HP E L L    G  + +   +T    I    +     I+
Sbjct: 144 NVHSVHHEYATPFGLTSEYAHPAEILFL----GFATIVGPALTGPHLITLWLWMMLRVIE 203

Query: 190 TVDDHCGL---WLPGNLFHLLFRNNTAYHDLHHQ-LYGNKVNYSQPFFVTWDRILGTYMP 237
           TV+ HCG    W P N   L       +HD HH+ LY    NYS   FV  D I GT   
Sbjct: 204 TVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSST-FVYMDWIFGTDKG 251

[Close]

BLAST of Carg25817 vs. TAIR 10
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-13
Identity = 73/251 (29.08%), Postives = 111/251 (44.22%), Query Frame = 0


Query: 10  LGTFVPIFVYWLYSGI-YVLL---GFFHNYRLHSKTEEDEKNLVSKSTVVRGVLFQQFVQ 69
           +G+F+     +  SG+ Y+ L   GF  NY++ +K+   E        + R +L+   V 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  ---AIVAIILFKVTGNEDGSSAVPKSWPMVALQFVTAMFVLDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G E  SS    SW +V+ Q +    + D   Y+ HR + H K+LY+
Sbjct: 84  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 143

Query: 130 HIHSHHHRLVVPYAFGALYNHPVEGLLLDMVGGVLSFLASGMTPRVSI---FFFSFATIK 189
           ++HS HH    P+   + Y HP E L L    G  + +   +T    I    +     I+
Sbjct: 144 NVHSVHHEYATPFGLTSEYAHPAEILFL----GFATIVGPALTGPHLITLWLWMMLRVIE 203

Query: 190 TVDDHCGL---WLPGNLFHLL----------FRNNTAYHDLHHQ-LYGNKVNYSQPFFVT 237
           TV+ HCG    W P N   L           F  +  +HD HH+ LY    NYS   FV 
Sbjct: 204 TVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYSST-FVY 263

The following BLAST results are available for this feature:

BLAST of Carg25817 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022946507.1	2.3e-144	100.00	sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >XP_022946508.1 sphinga...	[more]
XP_023546641.1	2.5e-143	99.60	sphinganine C4-monooxygenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022972857.1	4.3e-143	98.81	sphinganine C4-monooxygenase 1-like [Cucurbita maxima]	[more]
XP_008441706.1	2.5e-135	92.49	PREDICTED: sphinganine C4-monooxygenase 1-like [Cucumis melo] >KAA0032268.1 sphi...	[more]
XP_038889456.1	4.8e-134	91.70	sphinganine C4-monooxygenase 1-like [Benincasa hispida]	[more]

BLAST of Carg25817 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYI1	6.6e-110	75.20	Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1	[more]
Q9AST3	1.2e-106	71.71	Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1	[more]
B8B4W4	4.3e-93	66.54	Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39...	[more]
Q0D4G3	4.3e-93	66.54	Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=...	[more]
O94298	1.3e-54	43.08	Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]

BLAST of Carg25817 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G422	1.1e-144	100.00	sphinganine C4-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11145054...	[more]
A0A6J1I5Y6	2.1e-143	98.81	sphinganine C4-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471367 ...	[more]
A0A5D3CZU2	1.2e-135	92.49	Sphinganine C4-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3B426	1.2e-135	92.49	sphinganine C4-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103485785 PE=3...	[more]
A0A6J1DY75	2.3e-134	91.70	sphinganine C4-monooxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC1110256...	[more]

BLAST of Carg25817 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69640.1	4.7e-111	75.20	sphingoid base hydroxylase 1	[more]
AT1G14290.1	8.2e-108	71.71	sphingoid base hydroxylase 2	[more]
AT2G29390.3	6.9e-14	29.88	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.2	6.9e-14	29.88	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.1	2.0e-13	29.08	sterol 4-alpha-methyl-oxidase 2-2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 96..232 e-value: 2.7E-23 score: 82.8
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 3..249
None	No IPR available	PANTHER	PTHR11863:SF122	SPHINGANINE C4-MONOOXYGENASE 1-LIKE	coord: 3..249

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25817-RA	Carg25817-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016491	oxidoreductase activity