Carg25642 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25642
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontranscription factor bHLH147-like
LocationCarg_Chr06: 9562395 .. 9563039 (+)
RNA-Seq ExpressionCarg25642
SyntenyCarg25642
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCGTCTCTGTTTCTGAATCCTGTGGCGAGTTCTGAGCGATCGCGTGATTCGTCCAGAAAGAAAAAGAAGAAGAAAGCGGCGAGGGAGGACGGTCGTCAAGATCAGGGTCACTTGAAGTGGAAATCGCAAGCGCAGCATCAAATTTACTCGTCGAAGCTGTTGCGCGCGTTGAGTCAGGTCAGGATCAGTCCTCCTTATTCCCCGTCGGCGCCGAACGAGATGCCACGGCGTGGCCAAGCCGTTCGGGAGGCGGCGGATACGGTTCTGGCGATGACGGCGAAAGGAAGAAGTAGGTGGAGCAGAGCAATCTTGACGAACCGTCTGAAGCTCAAGTTTCGAAAGCACAATCGGCAGAAGGCGCGTGTGAGTGGAAGCAGCCGAACGAAGAAGCCGAGAGTTAGCGTTTTGCGGTTGAAAGGGAAGGGATTACCAACTGTACAGAGAAAAGTCCGGCTTCTCGGCCGGCTGGTTCCCGGCTGCCGGAAGCAACCGCTGCCGGTGATTCTGGATGAAGCAACTGATTACATACCCGCACTGGAGATGCAGATCCGAGTCATGAGCGCCATATTCAACCTGGTTTCAAGCTCTTCGTCTTCTCCTGGTATGGATTCTTCCATCTCCCAACCTTCTTCTACCTGA

mRNA sequence

ATGGCGTCGTCTCTGTTTCTGAATCCTGTGGCGAGTTCTGAGCGATCGCGTGATTCGTCCAGAAAGAAAAAGAAGAAGAAAGCGGCGAGGGAGGACGGTCGTCAAGATCAGGGTCACTTGAAGTGGAAATCGCAAGCGCAGCATCAAATTTACTCGTCGAAGCTGTTGCGCGCGTTGAGTCAGGTCAGGATCAGTCCTCCTTATTCCCCGTCGGCGCCGAACGAGATGCCACGGCGTGGCCAAGCCGTTCGGGAGGCGGCGGATACGGTTCTGGCGATGACGGCGAAAGGAAGAAGTAGGTGGAGCAGAGCAATCTTGACGAACCGTCTGAAGCTCAAGTTTCGAAAGCACAATCGGCAGAAGGCGCGTGTGAGTGGAAGCAGCCGAACGAAGAAGCCGAGAGTTAGCGTTTTGCGGTTGAAAGGGAAGGGATTACCAACTGTACAGAGAAAAGTCCGGCTTCTCGGCCGGCTGGTTCCCGGCTGCCGGAAGCAACCGCTGCCGGTGATTCTGGATGAAGCAACTGATTACATACCCGCACTGGAGATGCAGATCCGAGTCATGAGCGCCATATTCAACCTGGTTTCAAGCTCTTCGTCTTCTCCTGGTATGGATTCTTCCATCTCCCAACCTTCTTCTACCTGA

Coding sequence (CDS)

ATGGCGTCGTCTCTGTTTCTGAATCCTGTGGCGAGTTCTGAGCGATCGCGTGATTCGTCCAGAAAGAAAAAGAAGAAGAAAGCGGCGAGGGAGGACGGTCGTCAAGATCAGGGTCACTTGAAGTGGAAATCGCAAGCGCAGCATCAAATTTACTCGTCGAAGCTGTTGCGCGCGTTGAGTCAGGTCAGGATCAGTCCTCCTTATTCCCCGTCGGCGCCGAACGAGATGCCACGGCGTGGCCAAGCCGTTCGGGAGGCGGCGGATACGGTTCTGGCGATGACGGCGAAAGGAAGAAGTAGGTGGAGCAGAGCAATCTTGACGAACCGTCTGAAGCTCAAGTTTCGAAAGCACAATCGGCAGAAGGCGCGTGTGAGTGGAAGCAGCCGAACGAAGAAGCCGAGAGTTAGCGTTTTGCGGTTGAAAGGGAAGGGATTACCAACTGTACAGAGAAAAGTCCGGCTTCTCGGCCGGCTGGTTCCCGGCTGCCGGAAGCAACCGCTGCCGGTGATTCTGGATGAAGCAACTGATTACATACCCGCACTGGAGATGCAGATCCGAGTCATGAGCGCCATATTCAACCTGGTTTCAAGCTCTTCGTCTTCTCCTGGTATGGATTCTTCCATCTCCCAACCTTCTTCTACCTGA

Protein sequence

MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALSQVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQKARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPALEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST
Homology
BLAST of Carg25642 vs. NCBI nr
Match: KAG6597578.1 (Transcription factor basic helix-loop-helix 147, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029022.1 Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 389.4 bits (999), Expect = 2.0e-104
Identity = 214/214 (100.00%), Postives = 214/214 (100.00%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS
Sbjct: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ
Sbjct: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA
Sbjct: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180

Query: 181 LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST 215
           LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST
Sbjct: 181 LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST 214

BLAST of Carg25642 vs. NCBI nr
Match: XP_022938342.1 (transcription factor bHLH147-like [Cucurbita moschata] >XP_022938343.1 transcription factor bHLH147-like [Cucurbita moschata])

HSP 1 Score: 386.7 bits (992), Expect = 1.3e-103
Identity = 213/214 (99.53%), Postives = 213/214 (99.53%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQ HLKWKSQAQHQIYSSKLLRALS
Sbjct: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQDHLKWKSQAQHQIYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ
Sbjct: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA
Sbjct: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180

Query: 181 LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST 215
           LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST
Sbjct: 181 LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST 214

BLAST of Carg25642 vs. NCBI nr
Match: XP_023540934.1 (transcription factor bHLH147-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.6 bits (976), Expect = 9.1e-102
Identity = 210/214 (98.13%), Postives = 211/214 (98.60%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSLFLNPVASSERSRDSSRKKKKKK AREDGRQDQ HLKWKSQAQHQIYSSKLLRALS
Sbjct: 1   MASSLFLNPVASSERSRDSSRKKKKKKEAREDGRQDQDHLKWKSQAQHQIYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRIS PYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ
Sbjct: 61  QVRISSPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA
Sbjct: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180

Query: 181 LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST 215
           L+MQIRVMSAIFNLVSSSSSSPGMDSSISQPSST
Sbjct: 181 LDMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST 214

BLAST of Carg25642 vs. NCBI nr
Match: XP_022972020.1 (transcription factor bHLH147-like [Cucurbita maxima] >XP_022972021.1 transcription factor bHLH147-like [Cucurbita maxima] >XP_022972022.1 transcription factor bHLH147-like [Cucurbita maxima])

HSP 1 Score: 376.7 bits (966), Expect = 1.3e-100
Identity = 210/215 (97.67%), Postives = 211/215 (98.14%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQ HLKWKSQAQHQ+YSSKLLRALS
Sbjct: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQNHLKWKSQAQHQVYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ
Sbjct: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KA VSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA
Sbjct: 121 KASVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180

Query: 181 LEMQIRVMSAIFNLV-SSSSSSPGMDSSISQPSST 215
           LEMQIRVMSAIFNLV SSSSSS GMDSSISQPSST
Sbjct: 181 LEMQIRVMSAIFNLVSSSSSSSSGMDSSISQPSST 215

BLAST of Carg25642 vs. NCBI nr
Match: KAG7018561.1 (Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 328.6 bits (841), Expect = 4.1e-86
Identity = 183/212 (86.32%), Postives = 195/212 (91.98%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASS+ LNPVASSERSRDSSRKKKKKKAARE+ RQ Q H++WKSQAQHQ+YSSKLLRALS
Sbjct: 1   MASSMILNPVASSERSRDSSRKKKKKKAARENDRQGQDHIQWKSQAQHQVYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRIS   S SAPNE PRRG+AVREA+DTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ
Sbjct: 61  QVRISS--SESAPNETPRRGRAVREASDTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KA  +G+SRTKKPRVSV RL+GKGLPT+Q K RLLGRLVPGCRKQPLPVILDE TDYIPA
Sbjct: 121 KAHATGNSRTKKPRVSVFRLRGKGLPTLQSKARLLGRLVPGCRKQPLPVILDEVTDYIPA 180

Query: 181 LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPS 213
           LEMQIRVMSAI NLVSSSSSSPGMDSSIS P+
Sbjct: 181 LEMQIRVMSAIVNLVSSSSSSPGMDSSISPPT 210

BLAST of Carg25642 vs. ExPASy Swiss-Prot
Match: Q9LSN7 (Transcription factor bHLH147 OS=Arabidopsis thaliana OX=3702 GN=BHLH147 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.1e-44
Identity = 109/210 (51.90%), Postives = 142/210 (67.62%), Query Frame = 0

Query: 3   SSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHL---KWKSQAQHQIYSSKLLRAL 62
           S+  L P  +S  S  S RK+KKK +     +     +   KW+S+ Q QIYS+KL+ AL
Sbjct: 8   SNQLLQPTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHAL 67

Query: 63  SQVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNR 122
            ++RIS   S S+ + +PR G+AVRE AD  LA+ A+G++ WSRAIL+  +KLKFRKH R
Sbjct: 68  RELRISQQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKR 127

Query: 123 QK-------ARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILD 182
           Q+          +GS R+KK R +VLRLK KGLP VQRKV++L RLVPGCRKQ LPV+L+
Sbjct: 128 QRISNPTTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLE 187

Query: 183 EATDYIPALEMQIRVMSAIFNLVSSSSSSP 203
           E TDYI A+EMQIR M+AI + VSSS   P
Sbjct: 188 ETTDYIAAMEMQIRTMTAILSAVSSSPPPP 217

BLAST of Carg25642 vs. ExPASy Swiss-Prot
Match: Q9C8Z9 (Transcription factor bHLH148 OS=Arabidopsis thaliana OX=3702 GN=BHLH148 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-40
Identity = 103/203 (50.74%), Postives = 138/203 (67.98%), Query Frame = 0

Query: 10  VASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALSQVRISPPYS 69
           V   +RS  SS    +  A+   G   + H +W+S+ Q +IYS+KL +AL QVR++   S
Sbjct: 19  VRRKKRSSASSAASSRSSASSVSG---EIHARWRSEKQQRIYSAKLFQALQQVRLNSSAS 78

Query: 70  PSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQKARV----- 129
            S+     +RG+AVREAAD  LA++A+GR+ WSRAIL NR+KLKFRK  R +A +     
Sbjct: 79  TSSSPTAQKRGKAVREAADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMAIPAM 138

Query: 130 -----SGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIP 189
                S S+R++K RVSVLRL  K +P V RKVR+LGRLVPGC KQ +PVIL+EATDYI 
Sbjct: 139 TTVVSSSSNRSRKRRVSVLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEATDYIQ 198

Query: 190 ALEMQIRVMSAIFNLVSSSSSSP 203
           ALEMQ+R M+++  L+SS  S+P
Sbjct: 199 ALEMQVRAMNSLVQLLSSYGSAP 218

BLAST of Carg25642 vs. ExPASy Swiss-Prot
Match: O80482 (Transcription factor bHLH149 OS=Arabidopsis thaliana OX=3702 GN=BHLH149 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.1e-25
Identity = 87/209 (41.63%), Postives = 132/209 (63.16%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKK-----KAAREDGRQDQGHLK-WKSQAQHQIYSSK 60
           M  SLF     S E + +SSR+KK +     +A  E  R ++  LK WK+    QIY+ K
Sbjct: 1   MVESLF----PSIENTGESSRRKKPRISETAEAEIEARRVNEESLKRWKTNRVQQIYACK 60

Query: 61  LLRALSQVRISPPYSPSAPNEMPR----RGQAVREAADTVLAMTAKGRSRWSRAILTNRL 120
           L+ AL +VR     S ++ NE  +      + +R+ AD VLA +A+G +RWSRAIL +R+
Sbjct: 61  LVEALRRVR--QRSSTTSNNETDKLVSGAAREIRDTADRVLAASARGTTRWSRAILASRV 120

Query: 121 KLKFRKHNRQKARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVI 180
           + K +KH + K + +G+ +++K      R+K   LP V+RK+++LGRLVPGCRK  +P +
Sbjct: 121 RAKLKKHRKAK-KSTGNCKSRKGLTETNRIK---LPAVERKLKILGRLVPGCRKVSVPNL 180

Query: 181 LDEATDYIPALEMQIRVMSAIFNLVSSSS 200
           LDEATDYI ALEMQ+R M A+  L+++++
Sbjct: 181 LDEATDYIAALEMQVRAMEALAELLTAAA 199

BLAST of Carg25642 vs. ExPASy Swiss-Prot
Match: Q9M9L6 (Transcription factor bHLH150 OS=Arabidopsis thaliana OX=3702 GN=BHLH150 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.7e-16
Identity = 69/194 (35.57%), Postives = 99/194 (51.03%), Query Frame = 0

Query: 15  RSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALSQVRISPPYSPSAPN 74
           RSR SS +   +  +R          +W++    ++YS KL  AL               
Sbjct: 46  RSRVSSEEAPVRHLSR----------RWRATTAQKVYSLKLYDAL--------------- 105

Query: 75  EMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQKARVSGSSRTKKPR 134
           +  RR   VR+ AD VLA TA+G +RWSRAIL +R     R+    K   + ++  +   
Sbjct: 106 QRSRRSATVRDTADKVLATTARGATRWSRAILVSRFGTSLRRRRNTKPASALAAAIRGSG 165

Query: 135 VSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPALEMQIRVMSAIFNL 194
            S  R K   L  V  +VR+LG LVPGCR+  LP +LDE  DYI ALEMQ+R M+A+  +
Sbjct: 166 GSGRRRK---LSAVGNRVRVLGGLVPGCRRTALPELLDETADYIAALEMQVRAMTALSKI 211

Query: 195 VSSSSSSPGMDSSI 209
           +S    S  + S++
Sbjct: 226 LSELQPSTNLGSAL 211

BLAST of Carg25642 vs. ExPASy Swiss-Prot
Match: Q9SKX1 (Transcription factor IBH1 OS=Arabidopsis thaliana OX=3702 GN=IBH1 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 3.1e-04
Identity = 41/151 (27.15%), Postives = 72/151 (47.68%), Query Frame = 0

Query: 47  QHQIYSSKLLRALSQVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSR-WSRAI 106
           +  +++   L++LS +R   P+    P++   R + +++AA   +A  A G SR WSRA+
Sbjct: 14  EKDVFAFHFLQSLSNLRKQNPF--DTPDQKNYRVRKIKKAAYVSMARAAGGSSRLWSRAL 73

Query: 107 LTNRLKLKFRKHNRQKARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQ 166
           L        R+ ++   ++   SR K    S  R   +  P V+     L  LVPG    
Sbjct: 74  L--------RRADKDDNKIVRFSRRKWKISSKRRRSNQRAPVVEEAAERLRNLVPGGGGM 133

Query: 167 PLPVILDEATDYIPALEMQIRVMSAIFNLVS 197
               +++E   YI  L MQ++VM  + + +S
Sbjct: 134 ETSKLMEETAHYIKCLSMQVKVMQCLVDGLS 154

BLAST of Carg25642 vs. ExPASy TrEMBL
Match: A0A6J1FIM0 (transcription factor bHLH147-like OS=Cucurbita moschata OX=3662 GN=LOC111444503 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 6.2e-104
Identity = 213/214 (99.53%), Postives = 213/214 (99.53%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQ HLKWKSQAQHQIYSSKLLRALS
Sbjct: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQDHLKWKSQAQHQIYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ
Sbjct: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA
Sbjct: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180

Query: 181 LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST 215
           LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST
Sbjct: 181 LEMQIRVMSAIFNLVSSSSSSPGMDSSISQPSST 214

BLAST of Carg25642 vs. ExPASy TrEMBL
Match: A0A6J1I7C8 (transcription factor bHLH147-like OS=Cucurbita maxima OX=3661 GN=LOC111470680 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.4e-101
Identity = 210/215 (97.67%), Postives = 211/215 (98.14%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQ HLKWKSQAQHQ+YSSKLLRALS
Sbjct: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQNHLKWKSQAQHQVYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ
Sbjct: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KA VSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA
Sbjct: 121 KASVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180

Query: 181 LEMQIRVMSAIFNLV-SSSSSSPGMDSSISQPSST 215
           LEMQIRVMSAIFNLV SSSSSS GMDSSISQPSST
Sbjct: 181 LEMQIRVMSAIFNLVSSSSSSSSGMDSSISQPSST 215

BLAST of Carg25642 vs. ExPASy TrEMBL
Match: A0A1S3AY92 (transcription factor bHLH147-like OS=Cucumis melo OX=3656 GN=LOC103483901 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.1e-84
Identity = 187/215 (86.98%), Postives = 195/215 (90.70%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSL LNPV SSERSRDSSRKKKKKKA RED RQDQ  +KWKSQAQHQ+YSSKLLRALS
Sbjct: 1   MASSLILNPVTSSERSRDSSRKKKKKKATREDDRQDQDQIKWKSQAQHQVYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRIS P     PNE PRRG+AVREA+DTVLAMTAKGRSRWSRAILTNRLKLKFRKHN+Q
Sbjct: 61  QVRISSP--EPTPNETPRRGRAVREASDTVLAMTAKGRSRWSRAILTNRLKLKFRKHNKQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KARV G+SRTKKP VSVLRL+GKGLPTVQRKVRLLGRLVPGCRKQPLPVILDE TDYIPA
Sbjct: 121 KARVMGNSRTKKPSVSVLRLRGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEVTDYIPA 180

Query: 181 LEMQIRVMSAIFNLV-SSSSSSPGMDSSISQPSST 215
           LEMQIRVMSAI NLV SSSSSS G+DSSIS PSST
Sbjct: 181 LEMQIRVMSAIVNLVSSSSSSSSGIDSSIS-PSST 212

BLAST of Carg25642 vs. ExPASy TrEMBL
Match: A0A5D3CWF6 (Transcription factor bHLH147-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001600 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.4e-84
Identity = 187/215 (86.98%), Postives = 195/215 (90.70%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSL LNPV SSERSRDSSRKKKKKKA RED RQDQ  +KWKSQAQHQ+YSSKLLRALS
Sbjct: 1   MASSLILNPVTSSERSRDSSRKKKKKKATREDDRQDQDQIKWKSQAQHQVYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRIS P     PNE PRRG+AVREA+DTVLAMTAKGRSRWSRAILTNRLKLKFRKHN+Q
Sbjct: 61  QVRISSP--EPKPNETPRRGRAVREASDTVLAMTAKGRSRWSRAILTNRLKLKFRKHNKQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KARV G+SRTKKP VSVLRL+GKGLPTVQRKVRLLGRLVPGCRKQPLPVILDE TDYIPA
Sbjct: 121 KARVMGNSRTKKPSVSVLRLRGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEVTDYIPA 180

Query: 181 LEMQIRVMSAIFNLV-SSSSSSPGMDSSISQPSST 215
           LEMQIRVMSAI NLV SSSSSS G+DSSIS PSST
Sbjct: 181 LEMQIRVMSAIVNLVSSSSSSSSGIDSSIS-PSST 212

BLAST of Carg25642 vs. ExPASy TrEMBL
Match: A0A0A0L965 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G168390 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.2e-84
Identity = 186/215 (86.51%), Postives = 194/215 (90.23%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALS 60
           MASSL LNPV SSERSRDSSRKKKKKKA RED RQDQ  +KWKSQAQHQ+YSSKLLRALS
Sbjct: 1   MASSLILNPVTSSERSRDSSRKKKKKKATREDDRQDQDQIKWKSQAQHQVYSSKLLRALS 60

Query: 61  QVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQ 120
           QVRIS P     PNE PRRG+AVREA+DTVLAMTAKGRSRWSRAILTNRLKLKFRKHN+Q
Sbjct: 61  QVRISSP--EPTPNETPRRGRAVREASDTVLAMTAKGRSRWSRAILTNRLKLKFRKHNKQ 120

Query: 121 KARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIPA 180
           KARV G+SRTKKP VSVLRL+GKGLPTVQRKVRLLGRLVPGCRKQPLPVILDE TDYIPA
Sbjct: 121 KARVIGNSRTKKPSVSVLRLRGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEVTDYIPA 180

Query: 181 LEMQIRVMSAIFNLV-SSSSSSPGMDSSISQPSST 215
           LEMQIRVMSAI NLV SSSSSS G+DSSI  PSST
Sbjct: 181 LEMQIRVMSAIVNLVSSSSSSSTGIDSSI-PPSST 212

BLAST of Carg25642 vs. TAIR 10
Match: AT3G17100.1 (sequence-specific DNA binding transcription factors )

HSP 1 Score: 179.9 bits (455), Expect = 2.2e-45
Identity = 109/210 (51.90%), Postives = 142/210 (67.62%), Query Frame = 0

Query: 3   SSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHL---KWKSQAQHQIYSSKLLRAL 62
           S+  L P  +S  S  S RK+KKK +     +     +   KW+S+ Q QIYS+KL+ AL
Sbjct: 8   SNQLLQPTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHAL 67

Query: 63  SQVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNR 122
            ++RIS   S S+ + +PR G+AVRE AD  LA+ A+G++ WSRAIL+  +KLKFRKH R
Sbjct: 68  RELRISQQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKR 127

Query: 123 QK-------ARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILD 182
           Q+          +GS R+KK R +VLRLK KGLP VQRKV++L RLVPGCRKQ LPV+L+
Sbjct: 128 QRISNPTTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLE 187

Query: 183 EATDYIPALEMQIRVMSAIFNLVSSSSSSP 203
           E TDYI A+EMQIR M+AI + VSSS   P
Sbjct: 188 ETTDYIAAMEMQIRTMTAILSAVSSSPPPP 217

BLAST of Carg25642 vs. TAIR 10
Match: AT3G17100.2 (sequence-specific DNA binding transcription factors )

HSP 1 Score: 179.9 bits (455), Expect = 2.2e-45
Identity = 109/210 (51.90%), Postives = 142/210 (67.62%), Query Frame = 0

Query: 3   SSLFLNPVASSERSRDSSRKKKKKKAAREDGRQDQGHL---KWKSQAQHQIYSSKLLRAL 62
           S+  L P  +S  S  S RK+KKK +     +     +   KW+S+ Q QIYS+KL+ AL
Sbjct: 8   SNQLLQPTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHAL 67

Query: 63  SQVRISPPYSPSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNR 122
            ++RIS   S S+ + +PR G+AVRE AD  LA+ A+G++ WSRAIL+  +KLKFRKH R
Sbjct: 68  RELRISQQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKR 127

Query: 123 QK-------ARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILD 182
           Q+          +GS R+KK R +VLRLK KGLP VQRKV++L RLVPGCRKQ LPV+L+
Sbjct: 128 QRISNPTTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLE 187

Query: 183 EATDYIPALEMQIRVMSAIFNLVSSSSSSP 203
           E TDYI A+EMQIR M+AI + VSSS   P
Sbjct: 188 ETTDYIAAMEMQIRTMTAILSAVSSSPPPP 217

BLAST of Carg25642 vs. TAIR 10
Match: AT3G06590.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 8.7e-42
Identity = 103/203 (50.74%), Postives = 138/203 (67.98%), Query Frame = 0

Query: 10  VASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALSQVRISPPYS 69
           V   +RS  SS    +  A+   G   + H +W+S+ Q +IYS+KL +AL QVR++   S
Sbjct: 19  VRRKKRSSASSAASSRSSASSVSG---EIHARWRSEKQQRIYSAKLFQALQQVRLNSSAS 78

Query: 70  PSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQKARV----- 129
            S+     +RG+AVREAAD  LA++A+GR+ WSRAIL NR+KLKFRK  R +A +     
Sbjct: 79  TSSSPTAQKRGKAVREAADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMAIPAM 138

Query: 130 -----SGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIP 189
                S S+R++K RVSVLRL  K +P V RKVR+LGRLVPGC KQ +PVIL+EATDYI 
Sbjct: 139 TTVVSSSSNRSRKRRVSVLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEATDYIQ 198

Query: 190 ALEMQIRVMSAIFNLVSSSSSSP 203
           ALEMQ+R M+++  L+SS  S+P
Sbjct: 199 ALEMQVRAMNSLVQLLSSYGSAP 218

BLAST of Carg25642 vs. TAIR 10
Match: AT3G06590.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 8.7e-42
Identity = 103/203 (50.74%), Postives = 138/203 (67.98%), Query Frame = 0

Query: 10  VASSERSRDSSRKKKKKKAAREDGRQDQGHLKWKSQAQHQIYSSKLLRALSQVRISPPYS 69
           V   +RS  SS    +  A+   G   + H +W+S+ Q +IYS+KL +AL QVR++   S
Sbjct: 19  VRRKKRSSASSAASSRSSASSVSG---EIHARWRSEKQQRIYSAKLFQALQQVRLNSSAS 78

Query: 70  PSAPNEMPRRGQAVREAADTVLAMTAKGRSRWSRAILTNRLKLKFRKHNRQKARV----- 129
            S+     +RG+AVREAAD  LA++A+GR+ WSRAIL NR+KLKFRK  R +A +     
Sbjct: 79  TSSSPTAQKRGKAVREAADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMAIPAM 138

Query: 130 -----SGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVILDEATDYIP 189
                S S+R++K RVSVLRL  K +P V RKVR+LGRLVPGC KQ +PVIL+EATDYI 
Sbjct: 139 TTVVSSSSNRSRKRRVSVLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEATDYIQ 198

Query: 190 ALEMQIRVMSAIFNLVSSSSSSP 203
           ALEMQ+R M+++  L+SS  S+P
Sbjct: 199 ALEMQVRAMNSLVQLLSSYGSAP 218

BLAST of Carg25642 vs. TAIR 10
Match: AT1G09250.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 7.9e-27
Identity = 87/209 (41.63%), Postives = 132/209 (63.16%), Query Frame = 0

Query: 1   MASSLFLNPVASSERSRDSSRKKKKK-----KAAREDGRQDQGHLK-WKSQAQHQIYSSK 60
           M  SLF     S E + +SSR+KK +     +A  E  R ++  LK WK+    QIY+ K
Sbjct: 1   MVESLF----PSIENTGESSRRKKPRISETAEAEIEARRVNEESLKRWKTNRVQQIYACK 60

Query: 61  LLRALSQVRISPPYSPSAPNEMPR----RGQAVREAADTVLAMTAKGRSRWSRAILTNRL 120
           L+ AL +VR     S ++ NE  +      + +R+ AD VLA +A+G +RWSRAIL +R+
Sbjct: 61  LVEALRRVR--QRSSTTSNNETDKLVSGAAREIRDTADRVLAASARGTTRWSRAILASRV 120

Query: 121 KLKFRKHNRQKARVSGSSRTKKPRVSVLRLKGKGLPTVQRKVRLLGRLVPGCRKQPLPVI 180
           + K +KH + K + +G+ +++K      R+K   LP V+RK+++LGRLVPGCRK  +P +
Sbjct: 121 RAKLKKHRKAK-KSTGNCKSRKGLTETNRIK---LPAVERKLKILGRLVPGCRKVSVPNL 180

Query: 181 LDEATDYIPALEMQIRVMSAIFNLVSSSS 200
           LDEATDYI ALEMQ+R M A+  L+++++
Sbjct: 181 LDEATDYIAALEMQVRAMEALAELLTAAA 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6597578.12.0e-104100.00Transcription factor basic helix-loop-helix 147, partial [Cucurbita argyrosperma... [more]
XP_022938342.11.3e-10399.53transcription factor bHLH147-like [Cucurbita moschata] >XP_022938343.1 transcrip... [more]
XP_023540934.19.1e-10298.13transcription factor bHLH147-like [Cucurbita pepo subsp. pepo][more]
XP_022972020.11.3e-10097.67transcription factor bHLH147-like [Cucurbita maxima] >XP_022972021.1 transcripti... [more]
KAG7018561.14.1e-8686.32Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9LSN73.1e-4451.90Transcription factor bHLH147 OS=Arabidopsis thaliana OX=3702 GN=BHLH147 PE=1 SV=... [more]
Q9C8Z91.2e-4050.74Transcription factor bHLH148 OS=Arabidopsis thaliana OX=3702 GN=BHLH148 PE=1 SV=... [more]
O804821.1e-2541.63Transcription factor bHLH149 OS=Arabidopsis thaliana OX=3702 GN=BHLH149 PE=1 SV=... [more]
Q9M9L62.7e-1635.57Transcription factor bHLH150 OS=Arabidopsis thaliana OX=3702 GN=BHLH150 PE=1 SV=... [more]
Q9SKX13.1e-0427.15Transcription factor IBH1 OS=Arabidopsis thaliana OX=3702 GN=IBH1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FIM06.2e-10499.53transcription factor bHLH147-like OS=Cucurbita moschata OX=3662 GN=LOC111444503 ... [more]
A0A6J1I7C86.4e-10197.67transcription factor bHLH147-like OS=Cucurbita maxima OX=3661 GN=LOC111470680 PE... [more]
A0A1S3AY921.1e-8486.98transcription factor bHLH147-like OS=Cucumis melo OX=3656 GN=LOC103483901 PE=4 S... [more]
A0A5D3CWF61.4e-8486.98Transcription factor bHLH147-like protein OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A0A0L9653.2e-8486.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G168390 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G17100.12.2e-4551.90sequence-specific DNA binding transcription factors [more]
AT3G17100.22.2e-4551.90sequence-specific DNA binding transcription factors [more]
AT3G06590.18.7e-4250.74basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G06590.28.7e-4250.74basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G09250.17.9e-2741.63basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..46
NoneNo IPR availablePANTHERPTHR33124:SF78TRANSCRIPTION FACTOR BHLH148-LIKE ISOFORM X1coord: 7..210
IPR044660Transcription factor IBH1-likePANTHERPTHR33124TRANSCRIPTION FACTOR IBH1-LIKE 1coord: 7..210
IPR044549Transcription factor IBH1-like, bHLH domainCDDcd11444bHLH_AtIBH1_likecoord: 137..191
e-value: 4.43159E-15
score: 64.9512
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 152..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25642-RACarg25642-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000976 transcription cis-regulatory region binding