Carg25618 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25618
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cysteine proteinase inhibitor 8-like
Location	Carg_Chr18: 5342750 .. 5343094 (+)
RNA-Seq Expression	Carg25618
Synteny	Carg25618

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATCCTACTCCGCTTCACTCTTCCTCGTCCTTCTCCTCCCGATCTTGGCCATCGCTCAAAACGAGAATCAAGTCGGCTCTTGGAAGCCGATTGCAGACGTGGATGATCCGCACATTCAAGAGATCGGAAAGTTCGCTGTGAAAGAGAACAACTTGGAATCCAAAAACAACTTCGTATACAAGAGGGTCGTTAGCGGCGAATCACAGGTCGCCTCTGGAATCAACTACCACCTCGTTATCGAGGTCAAGGATGGAAGTTTGGATGCGCAATATCAGGCCGTCGTGTTGGAGAAGAAATGGGAGCATTCCAAGCAGCTCGTGTCCTTCAAGCCTCTCTCCTAA

mRNA sequence

ATGAAATCCTACTCCGCTTCACTCTTCCTCGTCCTTCTCCTCCCGATCTTGGCCATCGCTCAAAACGAGAATCAAGTCGGCTCTTGGAAGCCGATTGCAGACGTGGATGATCCGCACATTCAAGAGATCGGAAAGTTCGCTGTGAAAGAGAACAACTTGGAATCCAAAAACAACTTCGTATACAAGAGGGTCGTTAGCGGCGAATCACAGGTCGCCTCTGGAATCAACTACCACCTCGTTATCGAGGTCAAGGATGGAAGTTTGGATGCGCAATATCAGGCCGTCGTGTTGGAGAAGAAATGGGAGCATTCCAAGCAGCTCGTGTCCTTCAAGCCTCTCTCCTAA

Coding sequence (CDS)

ATGAAATCCTACTCCGCTTCACTCTTCCTCGTCCTTCTCCTCCCGATCTTGGCCATCGCTCAAAACGAGAATCAAGTCGGCTCTTGGAAGCCGATTGCAGACGTGGATGATCCGCACATTCAAGAGATCGGAAAGTTCGCTGTGAAAGAGAACAACTTGGAATCCAAAAACAACTTCGTATACAAGAGGGTCGTTAGCGGCGAATCACAGGTCGCCTCTGGAATCAACTACCACCTCGTTATCGAGGTCAAGGATGGAAGTTTGGATGCGCAATATCAGGCCGTCGTGTTGGAGAAGAAATGGGAGCATTCCAAGCAGCTCGTGTCCTTCAAGCCTCTCTCCTAA

Protein sequence

MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFVYKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS

Homology

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BLAST of Carg25618 vs. NCBI nr
Match: KAG7012653.1 (Cysteine proteinase inhibitor 8, partial [Cucurbita argyrosperma subsp. argyrosperma] >KAG7012654.1 Cysteine proteinase inhibitor 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 224.6 bits (571), Expect = 4.5e-55
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV
Sbjct: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS
Sbjct: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 114

[Close]

BLAST of Carg25618 vs. NCBI nr
Match: KAG6573541.1 (Cysteine proteinase inhibitor 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 223.4 bits (568), Expect = 1.0e-54
Identity = 113/114 (99.12%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV
Sbjct: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YK+VVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS
Sbjct: 61  YKKVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 114

[Close]

BLAST of Carg25618 vs. NCBI nr
Match: XP_022925327.1 (cysteine proteinase inhibitor 8-like [Cucurbita moschata] >XP_022925350.1 cysteine proteinase inhibitor 8-like [Cucurbita moschata])

HSP 1 Score: 223.0 bits (567), Expect = 1.3e-54
Identity = 113/114 (99.12%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV
Sbjct: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YKRVVSG+SQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS
Sbjct: 61  YKRVVSGKSQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 114

[Close]

BLAST of Carg25618 vs. NCBI nr
Match: XP_023542104.1 (cysteine proteinase inhibitor 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 222.6 bits (566), Expect = 1.7e-54
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV
Sbjct: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSF PLS
Sbjct: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFNPLS 114

[Close]

BLAST of Carg25618 vs. NCBI nr
Match: KAG6573539.1 (Cysteine proteinase inhibitor 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 222.2 bits (565), Expect = 2.2e-54
Identity = 112/114 (98.25%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYSAS+FL LLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV
Sbjct: 1   MKSYSASIFLALLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS
Sbjct: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 114

[Close]

BLAST of Carg25618 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.3e-17
Identity = 51/114 (44.74%), Postives = 66/114 (57.89%), Query Frame = 0


Query: 8   LFLVLLLPILAIAQNE--------NQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNF 67
           L L LLL + A A  +          VG W PI DV DPHIQE+G +AV+ +   S +  
Sbjct: 6   LLLALLLAVSAAAAAQVGGNRGHGPLVGGWSPITDVGDPHIQELGGWAVERHASLSSDGL 65

Query: 68  VYKRVVSGESQVASGINYHLVIEVKD-GSLDAQYQAVVLEKKWEHSKQLVSFKP 113
            ++RV SGE QV SG+NY LV+   D     A Y AVV E+ W +++QL SFKP
Sbjct: 66  RFRRVTSGEQQVVSGMNYRLVVSASDPAGATASYVAVVYEQSWTNTRQLTSFKP 119

[Close]

BLAST of Carg25618 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 1.9e-16
Identity = 47/107 (43.93%), Postives = 68/107 (63.55%), Query Frame = 0


Query: 11  VLLLPILAIAQ---NENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFVYKRVVSG 70
           ++LLP++++ +       +GS KPI +V DP +  + K+A++E+N ESK   V+ +VV G
Sbjct: 11  LILLPLVSVVEGLGGGGGLGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEG 70

Query: 71  ESQVASGINYHLVIEVKDGSLDAQ-YQAVVLEKKWEHSKQLVSFKPL 114
            +QV SG  Y L I  KDG    + Y+AVV+EK W HSK L SFK L
Sbjct: 71  TTQVVSGTKYDLKIAAKDGGGKIKNYEAVVVEKLWLHSKSLESFKAL 117

[Close]

BLAST of Carg25618 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 3.3e-16
Identity = 46/104 (44.23%), Postives = 65/104 (62.50%), Query Frame = 0


Query: 10  LVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFVYKRVVSGES 69
           +V+LLP+ A A    +VG W PI++V DP + EIG+FAV E N  S++   ++ VVSGE+
Sbjct: 14  VVVLLPLYASA--AARVGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGET 73

Query: 70  QVASGINYHLVIEVKDG-SLDAQYQAVVLEKKWEHSKQLVSFKP 113
           QV SG NY L +   DG  +   Y A+V +K W   + L SF+P
Sbjct: 74  QVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Carg25618 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 9.5e-16
Identity = 42/108 (38.89%), Postives = 66/108 (61.11%), Query Frame = 0


Query: 7   SLFLVLLLPILAI---AQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFVYKR 66
           SL L+LLL + A     +     G W+PI +++   +Q++ +FAV E+N ++ +   Y+ 
Sbjct: 7   SLLLLLLLALSAAVVGGRKRVAAGGWRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQS 66

Query: 67  VVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFK 112
           VV G +QV +G NY LVI  KDG++   Y+AVV +K W H + L SF+
Sbjct: 67  VVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTSFR 114

[Close]

BLAST of Carg25618 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-15
Identity = 42/111 (37.84%), Postives = 65/111 (58.56%), Query Frame = 0


Query: 2   KSYSASLFLVLLLPILAIAQNE-NQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 61
           K  S  LFL+L L    +   +    G W+PI  ++   +Q++ +FAV E+N ++ +   
Sbjct: 4   KPLSLLLFLLLALSAAVVGGRKLVAAGGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQ 63

Query: 62  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFK 112
           Y+ VV G +QV +G NY LVI  KDG++   Y+AVV +K W H + L SF+
Sbjct: 64  YQSVVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTSFR 114

[Close]

BLAST of Carg25618 vs. ExPASy TrEMBL
Match: A0A6J1EBF5 (cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC111432616 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.3e-55
Identity = 113/114 (99.12%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV
Sbjct: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YKRVVSG+SQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS
Sbjct: 61  YKRVVSGKSQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 114

[Close]

BLAST of Carg25618 vs. ExPASy TrEMBL
Match: A0A6J1EBX5 (cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC111432638 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.8e-54
Identity = 112/114 (98.25%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYS SLFLVLLLPILAIAQN+NQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV
Sbjct: 1   MKSYSVSLFLVLLLPILAIAQNKNQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS
Sbjct: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 114

[Close]

BLAST of Carg25618 vs. ExPASy TrEMBL
Match: A0A6J1HSW8 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111466475 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.3e-47
Identity = 103/114 (90.35%), Postives = 107/114 (93.86%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYSASLFLVLLLPILAIAQNENQVG WKPIA+V+DP IQEIGKFAVKENNLESKN+ V
Sbjct: 1   MKSYSASLFLVLLLPILAIAQNENQVGFWKPIANVNDPQIQEIGKFAVKENNLESKNSLV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YKRVVSGESQVASGINY LVIEVKDGS DAQYQAVVLEKKWEHSKQLVSF  L+
Sbjct: 61  YKRVVSGESQVASGINYRLVIEVKDGSSDAQYQAVVLEKKWEHSKQLVSFTRLA 114

[Close]

BLAST of Carg25618 vs. ExPASy TrEMBL
Match: A0A6J1I9P5 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471323 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.2e-47
Identity = 102/114 (89.47%), Postives = 106/114 (92.98%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFV 60
           MKSYS SLFLVLLLPILAIAQNENQVGSWKPIA+V+DP IQEIGKFAVKENNLESKN+ V
Sbjct: 1   MKSYSTSLFLVLLLPILAIAQNENQVGSWKPIANVNDPQIQEIGKFAVKENNLESKNSLV 60

Query: 61  YKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
           YKRVVSGESQV SGINY LVIEVKDGS DAQYQAVVLEKKWEHSKQLVSF  L+
Sbjct: 61  YKRVVSGESQVVSGINYRLVIEVKDGSSDAQYQAVVLEKKWEHSKQLVSFTRLT 114

[Close]

BLAST of Carg25618 vs. ExPASy TrEMBL
Match: A0A6J1IC35 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.6e-26
Identity = 69/116 (59.48%), Postives = 82/116 (70.69%), Query Frame = 0


Query: 1   MKSYSASLFLVLLLPIL--AIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNN 60
           MKS SASLFL+LLLP+L  A A+    VG W+ I DV DPHIQEIGKFAV E N +SK  
Sbjct: 1   MKSRSASLFLILLLPLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGA 60

Query: 61  FVYKRVVSGESQVASGINYHLVIEVKDGSLDAQYQAVVLEKKWEHSKQLVSFKPLS 115
             +K VV GESQV SG+NY LVIE K G    +YQA+V EK W+H  +L SFKP++
Sbjct: 61  LEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALVWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Carg25618 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-17
Identity = 47/107 (43.93%), Postives = 68/107 (63.55%), Query Frame = 0


Query: 11  VLLLPILAIAQ---NENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFVYKRVVSG 70
           ++LLP++++ +       +GS KPI +V DP +  + K+A++E+N ESK   V+ +VV G
Sbjct: 11  LILLPLVSVVEGLGGGGGLGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEG 70

Query: 71  ESQVASGINYHLVIEVKDGSLDAQ-YQAVVLEKKWEHSKQLVSFKPL 114
            +QV SG  Y L I  KDG    + Y+AVV+EK W HSK L SFK L
Sbjct: 71  TTQVVSGTKYDLKIAAKDGGGKIKNYEAVVVEKLWLHSKSLESFKAL 117

[Close]

BLAST of Carg25618 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 2.3e-17
Identity = 46/104 (44.23%), Postives = 65/104 (62.50%), Query Frame = 0


Query: 10  LVLLLPILAIAQNENQVGSWKPIADVDDPHIQEIGKFAVKENNLESKNNFVYKRVVSGES 69
           +V+LLP+ A A    +VG W PI++V DP + EIG+FAV E N  S++   ++ VVSGE+
Sbjct: 14  VVVLLPLYASA--AARVGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGET 73

Query: 70  QVASGINYHLVIEVKDG-SLDAQYQAVVLEKKWEHSKQLVSFKP 113
           QV SG NY L +   DG  +   Y A+V +K W   + L SF+P
Sbjct: 74  QVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Carg25618 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 68.9 bits (167), Expect = 2.9e-12
Identity = 33/81 (40.74%), Postives = 50/81 (61.73%), Query Frame = 0


Query: 33  ADVDDPHIQEIGKFAVKENNLESKNNFVYKRVVSGESQVASGINYHLVIEVKDGSLDAQY 92
           A+ +D  ++ + +FAV E+N        YKR++  ++QV +G  +HL +EV DG  +  Y
Sbjct: 18  ANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVADGETNKVY 77

Query: 93  QAVVLEKKWEHSKQLVSFKPL 114
           +A VLEK WE+ KQL SF  L
Sbjct: 78  EAKVLEKAWENLKQLESFNHL 98

[Close]

BLAST of Carg25618 vs. TAIR 10
Match: AT2G31980.1 (PHYTOCYSTATIN 2 )

HSP 1 Score: 48.1 bits (113), Expect = 5.3e-06
Identity = 28/92 (30.43%), Postives = 49/92 (53.26%), Query Frame = 0


Query: 40  IQEIGKFAVKENNLESKNN-----------------FVYKRVVSGESQVASGINYHLVIE 99
           IQ++G++ V++ N +++N                    + RVVS + QV +G+ Y+L IE
Sbjct: 51  IQQLGRYCVEQFNQQAQNEQGNIGSIAKTDTAISNPLQFSRVVSAQKQVVAGLKYYLRIE 110

Query: 100 V-KDGSLDAQYQAVVLEKKWEHSKQLVSFKPL 114
           V +       + +VV+ + W HSKQL+ F P+
Sbjct: 111 VTQPNGSTRMFDSVVVIQPWLHSKQLLGFTPV 142

The following BLAST results are available for this feature:

BLAST of Carg25618 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012653.1	4.5e-55	100.00	Cysteine proteinase inhibitor 8, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6573541.1	1.0e-54	99.12	Cysteine proteinase inhibitor 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022925327.1	1.3e-54	99.12	cysteine proteinase inhibitor 8-like [Cucurbita moschata] >XP_022925350.1 cystei...	[more]
XP_023542104.1	1.7e-54	99.12	cysteine proteinase inhibitor 8-like [Cucurbita pepo subsp. pepo]	[more]
KAG6573539.1	2.2e-54	98.25	Cysteine proteinase inhibitor 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg25618 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10J94	2.3e-17	44.74	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
Q84WT8	1.9e-16	43.93	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]
Q41916	3.3e-16	44.23	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
P86472	9.5e-16	38.89	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q6TPK4	1.2e-15	37.84	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]

BLAST of Carg25618 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EBF5	6.3e-55	99.12	cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC1114326...	[more]
A0A6J1EBX5	1.8e-54	98.25	cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC1114326...	[more]
A0A6J1HSW8	1.3e-47	90.35	cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111466475...	[more]
A0A6J1I9P5	2.2e-47	89.47	cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471323...	[more]
A0A6J1IC35	1.6e-26	59.48	cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628...	[more]

BLAST of Carg25618 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16500.1	1.4e-17	43.93	Cystatin/monellin superfamily protein	[more]
AT5G47550.1	2.3e-17	44.23	Cystatin/monellin superfamily protein	[more]
AT5G12140.1	2.9e-12	40.74	cystatin-1	[more]
AT2G31980.1	5.3e-06	30.43	PHYTOCYSTATIN 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 24..113 e-value: 7.1E-17 score: 72.1
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 32..112 e-value: 1.2E-31 score: 108.8
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 27..112 e-value: 1.90579E-13 score: 59.41
None	No IPR available	GENE3D	3.10.450.10		coord: 19..114 e-value: 2.4E-30 score: 106.7
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 1..112
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 25..112
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 69..82

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25618-RA	Carg25618-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity