Carg25412 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25412
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING/U-box superfamily protein
LocationCarg_Chr18: 3746019 .. 3749249 (-)
RNA-Seq ExpressionCarg25412
SyntenyCarg25412
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTTTTTTTGTTTTTAATATCATTTCTTCCACAGACAAATCTTCCAGGCCACAGAATTCCCTTCACATGCGGTGAAGTTCTTATGGTATGGAACATTCATTCATTTCTTTGATTTCTTTTTTCTTCCGTTTCTTCTTTCTCCGACCCCCAATCCCGGAGTTTGTGTGAATTTTGGTTTGGTTCTTTTAAATGGGGTTTCGTGGATTAGGTAGTTGATTAATGGGTCGATCTTGATTTGCATAGAAAATACAGGAACTGCTTGTTTTGTTTGCGTTTTCTCCGATTCGGACATGTTCCTCTTTTTCGTATTCGATTTTTGGAATTTCGTGTCCTCTAATGTGGAAATGGAATGATTCTACGATGGGTATTCTGTTGCTTTTTGATCTAGAATATAGATTTGATAGAAATTTCAGAGTGATTTATATTGTTTTCTGTGATTTTAGGAGCGAATGTTGCTTCAGTTTGCAAATTAGGGAAATCTTGCTCCCGATTGTGTATTTGGAGTTCTTAAACAGCGGATGAGTAATTAAGAACTGCGAAGAACGTTCTTAAATTCATTTGCTGGTGGATCTTCGATTGTTTACTGCCATCTGGGATTTGTGGATCGTTTGAAATTTTTTAACAAAATGGAGAATCGTGAGATGTTTGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCATGAGGATGTGGTGGAAGAAGGGTCTGAGGAGGAGGAGGAGGAGGATCTTGATGAGTTTTCGGTGAAAATGTTCTTTAAAGGCGTTTCGATAGCCGAGATCGGTGATCCGTCTTCTGGTCTTTCTGGTATTGGAGTGTTCATGGAAAGATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTCTATGTTGATGAGCTAGTTGCTGATTATTTAGCTTTGATGGATGGATTGGTTGAGGCCATACAGAATGGCGTCCGCTCTATTCGAGCTTTTACCGACTCCAATACGTTATATAATCAGGTTATTACTTCCCCTACCTGCGTAACTTCGCTTGAAAGATTGCATAAACCCTTAAATTTATGTGCGTTTTCTGAAGATATGTGAATGTTTGACAGATAACAAATGAAGAGGAGCCTGAAATTCCGCTTCTTGTAGCGCTGAAAGAAAGGATTCTAGAGCATGCTAGCACTTTCGAAGCGTTTTCTCTGAACCTTTCGTCGCATTCGGACCTCGAGCAGCCATTTCATCTCGCGAAAGTGGCTATTGGGGTTCTTTCTTGTCCACAAAAGGGAGATACATCAGTTGAGAATTGTTCGATTTGTTGTGAGAACAAACCGACCGCTATGATGGTTGCGTTGAAGTGTTGTCATAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGCTGAGATGCAAATATTACATCACAAGCAATGAGTTTAAATCGTTTCTTCCCTTGACTTTATATGAATCGTTGGAGAATACCTTAGCCGAAGCGAATATTCACTCCGATAAAATTTACTGCCCTTATCCGAATTGCTCTGTTCTGTTAGACCCGAGCGAATGTTTGTCAGCTCGGGCAAGTTCGTCAAGTCAATCGGACAACACTTGTATTGAATGTCCAGTTTGTCAGAGGTTTATTTGTGTTGAATGTTGTGTTCCTTGGCATTCATCAATGAGCTGTGAAGAGTTTCAGGACATCCCATTAGAGGAAAGAGACATTGCTGATATTACCTTACATCGTCTTGCACGAAACAAAAGGTGGCGACGTTGTCAGCAGTGTCGTAGGATGATTGAATTGGCTCAAGGCTGCTACCATATGACATGCTGGTAATGCTACTTCCTCCAAATCTCTCATTATTTATGTTAACGTGTTAGTTTATGAGATAAATGTCGAAACACCGGAATCATCGTGAATATATGTCACAAGTTAAGTGGGATGTTAGATTTTTTTTTGAAGGATCGGCTTGTAGGACTTGATTAGAAAGTTAGAGGCACTTTAAATACTTATTTGCACGTCGTATACGTGATCTGATATTTGCACATATGGTCTATACATCTATGGTCAAGCTCATCTGGTTTGATATTTGCACGTCGTAAACGTGATCTGATATTTGCACGTATGTATGGTCTATGCATCTATGGCCAGGCACATGTGGTTTGATATTTGCATGTCGTAAACATGATCTGATATTTGCACGTATGGTCTATACATCTATGGCCAGGCTCATGTGGTTTGATATTTGCACGTCGTAAATGTGATCTGATATTTGCACGTATAGTCTATACATCTATGGCCAGGCACATGTGGTTTGATATTTGCACGTCGTAAACGTGATTTGATATTTACACGTCGTCAACGTGATTTGATATTTACACGTATGGTCTATAATCTATGGTCAGGCTCACGTGGTTTGATATTTGCACGTCGTAAACATGACGATTTTACTAAACTAGGTGCAGGGTTTTCTTTAGGTATATGAATTCAATGCTTATACTGATATGTTGCAGGTGCGGGCATGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGTCTTTTGGGACGACGATGACAACGACGACGATAACTCCGAGGACTTGGTTACCCACTCCATCCAAGAATCCGAGCAGTGGGCATGGGAAACTTTTAATTCCCTCCCGATGATAACGGACGCATACTCAGAGCAAGAGAGATCACAGTTGGCACTAATCCAACGATTCCTGGCTGGGGGATTCAGTCTGAGCGACCACCAACCGTATCAATCGCCGCCACGATGTACAGACTCTTACGTCGACACGATGAAGGACCTACACCAACTTCCATGGCTTGAGAGGTTCGTTTCCGTGATAAGTGATAACTACTATGAAGATTATGTACAATGATATCGATTCTTAGACAAAAAATAAAAGGGGGAGGTAAAAGAGTCTCTCACTCTAGTTTGCCTCCCTCGATACCTCATTTACAGTCCGCCGCACAGCTTGGCGTTTCGATCGTTAGTTACGACGCTGCTCTGTTTGTCTGTTAAATCATATTGAATCATATACTGGAAATTATGAAGTGACCCTCAGAGGTCCTCATTGCATTAGATTAGAATAGAATGAAGGAATGTTATGCAAGAATGTGAGCTTTGTCTGTTTGATTCTTTCCTGTTTCAAACAAAAGAAAAAGG

mRNA sequence

TTTTTTTTTTTTGTTTTTAATATCATTTCTTCCACAGACAAATCTTCCAGGCCACAGAATTCCCTTCACATGCGGTGAAGTTCTTATGGAGCGAATGTTGCTTCAGTTTGCAAATTAGGGAAATCTTGCTCCCGATTGTGTATTTGGAGTTCTTAAACAGCGGATGAGTAATTAAGAACTGCGAAGAACGTTCTTAAATTCATTTGCTGGTGGATCTTCGATTGTTTACTGCCATCTGGGATTTGTGGATCGTTTGAAATTTTTTAACAAAATGGAGAATCGTGAGATGTTTGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCATGAGGATGTGGTGGAAGAAGGGTCTGAGGAGGAGGAGGAGGAGGATCTTGATGAGTTTTCGGTGAAAATGTTCTTTAAAGGCGTTTCGATAGCCGAGATCGGTGATCCGTCTTCTGGTCTTTCTGGTATTGGAGTGTTCATGGAAAGATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTCTATGTTGATGAGCTAGTTGCTGATTATTTAGCTTTGATGGATGGATTGGTTGAGGCCATACAGAATGGCGTCCGCTCTATTCGAGCTTTTACCGACTCCAATACGTTATATAATCAGATAACAAATGAAGAGGAGCCTGAAATTCCGCTTCTTGTAGCGCTGAAAGAAAGGATTCTAGAGCATGCTAGCACTTTCGAAGCGTTTTCTCTGAACCTTTCGTCGCATTCGGACCTCGAGCAGCCATTTCATCTCGCGAAAGTGGCTATTGGGGTTCTTTCTTGTCCACAAAAGGGAGATACATCAGTTGAGAATTGTTCGATTTGTTGTGAGAACAAACCGACCGCTATGATGGTTGCGTTGAAGTGTTGTCATAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGCTGAGATGCAAATATTACATCACAAGCAATGAGTTTAAATCGTTTCTTCCCTTGACTTTATATGAATCGTTGGAGAATACCTTAGCCGAAGCGAATATTCACTCCGATAAAATTTACTGCCCTTATCCGAATTGCTCTGTTCTGTTAGACCCGAGCGAATGTTTGTCAGCTCGGGCAAGTTCGTCAAGTCAATCGGACAACACTTGTATTGAATGTCCAGTTTGTCAGAGGTTTATTTGTGTTGAATGTTGTGTTCCTTGGCATTCATCAATGAGCTGTGAAGAGTTTCAGGACATCCCATTAGAGGAAAGAGACATTGCTGATATTACCTTACATCGTCTTGCACGAAACAAAAGGTGGCGACGTTGTCAGCAGTGTCGTAGGATGATTGAATTGGCTCAAGGCTGCTACCATATGACATGCTGGTGCGGGCATGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGTCTTTTGGGACGACGATGACAACGACGACGATAACTCCGAGGACTTGGTTACCCACTCCATCCAAGAATCCGAGCAGTGGGCATGGGAAACTTTTAATTCCCTCCCGATGATAACGGACGCATACTCAGAGCAAGAGAGATCACAGTTGGCACTAATCCAACGATTCCTGGCTGGGGGATTCAGTCTGAGCGACCACCAACCGTATCAATCGCCGCCACGATGTACAGACTCTTACGTCGACACGATGAAGGACCTACACCAACTTCCATGGCTTGAGAGGTTCGTTTCCGTGATAAGTGATAACTACTATGAAGATTATGTACAATGATATCGATTCTTAGACAAAAAATAAAAGGGGGAGGTAAAAGAGTCTCTCACTCTAGTTTGCCTCCCTCGATACCTCATTTACAGTCCGCCGCACAGCTTGGCGTTTCGATCGTTAGTTACGACGCTGCTCTGTTTGTCTGTTAAATCATATTGAATCATATACTGGAAATTATGAAGTGACCCTCAGAGGTCCTCATTGCATTAGATTAGAATAGAATGAAGGAATGTTATGCAAGAATGTGAGCTTTGTCTGTTTGATTCTTTCCTGTTTCAAACAAAAGAAAAAGG

Coding sequence (CDS)

ATGGAGAATCGTGAGATGTTTGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCATGAGGATGTGGTGGAAGAAGGGTCTGAGGAGGAGGAGGAGGAGGATCTTGATGAGTTTTCGGTGAAAATGTTCTTTAAAGGCGTTTCGATAGCCGAGATCGGTGATCCGTCTTCTGGTCTTTCTGGTATTGGAGTGTTCATGGAAAGATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTCTATGTTGATGAGCTAGTTGCTGATTATTTAGCTTTGATGGATGGATTGGTTGAGGCCATACAGAATGGCGTCCGCTCTATTCGAGCTTTTACCGACTCCAATACGTTATATAATCAGATAACAAATGAAGAGGAGCCTGAAATTCCGCTTCTTGTAGCGCTGAAAGAAAGGATTCTAGAGCATGCTAGCACTTTCGAAGCGTTTTCTCTGAACCTTTCGTCGCATTCGGACCTCGAGCAGCCATTTCATCTCGCGAAAGTGGCTATTGGGGTTCTTTCTTGTCCACAAAAGGGAGATACATCAGTTGAGAATTGTTCGATTTGTTGTGAGAACAAACCGACCGCTATGATGGTTGCGTTGAAGTGTTGTCATAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGCTGAGATGCAAATATTACATCACAAGCAATGAGTTTAAATCGTTTCTTCCCTTGACTTTATATGAATCGTTGGAGAATACCTTAGCCGAAGCGAATATTCACTCCGATAAAATTTACTGCCCTTATCCGAATTGCTCTGTTCTGTTAGACCCGAGCGAATGTTTGTCAGCTCGGGCAAGTTCGTCAAGTCAATCGGACAACACTTGTATTGAATGTCCAGTTTGTCAGAGGTTTATTTGTGTTGAATGTTGTGTTCCTTGGCATTCATCAATGAGCTGTGAAGAGTTTCAGGACATCCCATTAGAGGAAAGAGACATTGCTGATATTACCTTACATCGTCTTGCACGAAACAAAAGGTGGCGACGTTGTCAGCAGTGTCGTAGGATGATTGAATTGGCTCAAGGCTGCTACCATATGACATGCTGGTGCGGGCATGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGTCTTTTGGGACGACGATGACAACGACGACGATAACTCCGAGGACTTGGTTACCCACTCCATCCAAGAATCCGAGCAGTGGGCATGGGAAACTTTTAATTCCCTCCCGATGATAACGGACGCATACTCAGAGCAAGAGAGATCACAGTTGGCACTAATCCAACGATTCCTGGCTGGGGGATTCAGTCTGAGCGACCACCAACCGTATCAATCGCCGCCACGATGTACAGACTCTTACGTCGACACGATGAAGGACCTACACCAACTTCCATGGCTTGAGAGGTTCGTTTCCGTGATAAGTGATAACTACTATGAAGATTATGTACAATGA

Protein sequence

MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Homology
BLAST of Carg25412 vs. NCBI nr
Match: KAG7012597.1 (putative E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1058.9 bits (2737), Expect = 1.4e-305
Identity = 512/512 (100.00%), Postives = 512/512 (100.00%), Query Frame = 0

Query: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60
           MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD
Sbjct: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60

Query: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120
           PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT
Sbjct: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120

Query: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180
           DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV
Sbjct: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180

Query: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240
           LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL
Sbjct: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240

Query: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300
           RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS
Sbjct: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300

Query: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360
           SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR
Sbjct: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360

Query: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV 420
           RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV
Sbjct: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV 420

Query: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480
           THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480

Query: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 512

BLAST of Carg25412 vs. NCBI nr
Match: KAG6573449.1 (putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1049.7 bits (2713), Expect = 8.4e-303
Identity = 509/512 (99.41%), Postives = 510/512 (99.61%), Query Frame = 0

Query: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60
           MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGS  +EEEDLDEFSVKMFFKGVSIAEIGD
Sbjct: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGS--KEEEDLDEFSVKMFFKGVSIAEIGD 60

Query: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120
           PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT
Sbjct: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120

Query: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180
           DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV
Sbjct: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180

Query: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240
           LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL
Sbjct: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240

Query: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300
           RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS
Sbjct: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300

Query: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360
           SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR
Sbjct: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360

Query: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV 420
           RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV
Sbjct: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV 420

Query: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480
           THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480

Query: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510

BLAST of Carg25412 vs. NCBI nr
Match: XP_022994789.1 (probable E3 ubiquitin-protein ligase ARI11 [Cucurbita maxima])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.5e-299
Identity = 504/512 (98.44%), Postives = 507/512 (99.02%), Query Frame = 0

Query: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60
           ME+REMFGPAEEDDVEDFRSCCEDHEDVVEEGS  EEEEDLDEFSVKMFFKG+SIAEIGD
Sbjct: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGS--EEEEDLDEFSVKMFFKGISIAEIGD 60

Query: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120
           PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT
Sbjct: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120

Query: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180
           DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV
Sbjct: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180

Query: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240
           LSCPQKGD S+ENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL
Sbjct: 181 LSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240

Query: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300
           RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS
Sbjct: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300

Query: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360
           SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR
Sbjct: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360

Query: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV 420
           RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQC FWDDDD DDDNSEDLV
Sbjct: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD-DDDNSEDLV 420

Query: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480
           THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480

Query: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 509

BLAST of Carg25412 vs. NCBI nr
Match: XP_022954734.1 (probable E3 ubiquitin-protein ligase ARI11 [Cucurbita moschata])

HSP 1 Score: 1037.3 bits (2681), Expect = 4.3e-299
Identity = 505/514 (98.25%), Postives = 506/514 (98.44%), Query Frame = 0

Query: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60
           MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGS  EEEEDLDEFSVKMFFKGVSIAE+GD
Sbjct: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGS--EEEEDLDEFSVKMFFKGVSIAEVGD 60

Query: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120
           PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT
Sbjct: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120

Query: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180
           DSNTLYNQITNEEEPEIPLLVALKERILEH STFEAFSLNLSSHSDLEQPFHLAKVAIGV
Sbjct: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHTSTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180

Query: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240
           LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL
Sbjct: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240

Query: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300
           RCKYYITSNEFKSFLPLTLYE LENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS
Sbjct: 241 RCKYYITSNEFKSFLPLTLYELLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300

Query: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360
           SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR
Sbjct: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360

Query: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDN--DDDNSED 420
           RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQD QQTCQC FWDDDDN  DDDNSED
Sbjct: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDEQQTCQCAFWDDDDNDDDDDNSED 420

Query: 421 LVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRC 480
           LVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRC
Sbjct: 421 LVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRC 480

Query: 481 TDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           TDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 TDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 512

BLAST of Carg25412 vs. NCBI nr
Match: XP_023541881.1 (probable E3 ubiquitin-protein ligase ARI10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1024.2 bits (2647), Expect = 3.8e-295
Identity = 498/512 (97.27%), Postives = 501/512 (97.85%), Query Frame = 0

Query: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60
           ME REMFGPAEEDDVEDFRSCCEDHEDVVE+GS  EEE+DLDEFSVKMFFKGVSIAEIGD
Sbjct: 1   MERREMFGPAEEDDVEDFRSCCEDHEDVVEDGS--EEEDDLDEFSVKMFFKGVSIAEIGD 60

Query: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120
           PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT
Sbjct: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120

Query: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180
           DSNTLYNQITNEEEPEIPLLVALKERILEHAST EAFSLNLSSHSDLEQPFHLAKVAIGV
Sbjct: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTCEAFSLNLSSHSDLEQPFHLAKVAIGV 180

Query: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240
           LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL
Sbjct: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240

Query: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300
           RCKYYITSNEFKSFLPLT YESLEN LAEANIHSDKIYCPYPNCS+LLDPSECLSARASS
Sbjct: 241 RCKYYITSNEFKSFLPLTSYESLENALAEANIHSDKIYCPYPNCSILLDPSECLSARASS 300

Query: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360
           SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR
Sbjct: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360

Query: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV 420
           RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQC FWD    DDDNSEDLV
Sbjct: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWD----DDDNSEDLV 420

Query: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480
           THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480

Query: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 506

BLAST of Carg25412 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-17
Identity = 61/204 (29.90%), Postives = 92/204 (45.10%), Query Frame = 0

Query: 194 CSICCENKPTAMMVALKCCHK-FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFK 253
           C +C E+KP   +  L CC K  C  C+K Y+  +++  QV I+CP   C  ++   E  
Sbjct: 263 CRVCLEDKP---IKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFL--EETT 322

Query: 254 SFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECP 313
               LT  +S++          D    P P C       +       S S+S    I+CP
Sbjct: 323 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYK-IQCP 382

Query: 314 VCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELA 373
            CQ   C +C  PWH  ++C+E++      R  A    H     +  ++C +C+  I+  
Sbjct: 383 TCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 442

Query: 374 QGCYHMTC-WCGHEFCYSCGAEYQ 396
           +GC HMTC  C   FCY CG  Y+
Sbjct: 443 EGCDHMTCSQCNTNFCYRCGERYR 457

BLAST of Carg25412 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-17
Identity = 61/204 (29.90%), Postives = 92/204 (45.10%), Query Frame = 0

Query: 194 CSICCENKPTAMMVALKCCHK-FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFK 253
           C +C E+KP   +  L CC K  C  C+K Y+  +++  QV I+CP   C  ++   E  
Sbjct: 236 CRVCLEDKP---IKPLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFL--EETT 295

Query: 254 SFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECP 313
               LT  +S++          D    P P C       +       S S+S    I+CP
Sbjct: 296 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSES-RYKIQCP 355

Query: 314 VCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELA 373
            CQ   C +C  PWH  ++C+E++      R  A    H     +  ++C +C+  I+  
Sbjct: 356 TCQLIWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 415

Query: 374 QGCYHMTC-WCGHEFCYSCGAEYQ 396
           +GC HMTC  C   FCY CG  Y+
Sbjct: 416 EGCDHMTCSQCNTNFCYRCGERYR 430

BLAST of Carg25412 vs. ExPASy Swiss-Prot
Match: Q9SKC3 (Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI9 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.9e-15
Identity = 55/214 (25.70%), Postives = 88/214 (41.12%), Query Frame = 0

Query: 194 CSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQ--VPIRCPQLRCKYYITSNEF 253
           C IC E+     +  + C H +C  C   Y+  K+E     + ++CP+  C   +  +  
Sbjct: 128 CGICFESYTREEIARVSCGHPYCKTCWAGYITTKIEDGPGCLRVKCPEPSCSAAVGKDMI 187

Query: 254 KSFLPLTLYESLENTLAEANIHSDK--IYCPYPNCSVLLDPSECLSARASSSSQSDNTCI 313
           +      + E     +  + +   K   +CP P C   ++         S SS  D +C+
Sbjct: 188 EDVTETKVNEKYSRYILRSYVEDGKKIKWCPSPGCGYAVE------FGGSESSSYDVSCL 247

Query: 314 ECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMI 373
               C    C  C    HS + C+       + +D ++     LA +K    C +C+R I
Sbjct: 248 ----CSYRFCWNCSEDAHSPVDCDTVSKWIFKNQDESENKNWMLANSK---PCPECKRPI 307

Query: 374 ELAQGCYHMTCW--CGHEFCYSCGAEYQDGQQTC 402
           E   GC HMTC   CGHEFC+ C   Y+     C
Sbjct: 308 EKNDGCNHMTCSAPCGHEFCWICLKAYRRHSGAC 328

BLAST of Carg25412 vs. ExPASy Swiss-Prot
Match: Q9SKC4 (Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=ARI10 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 7.2e-15
Identity = 61/233 (26.18%), Postives = 93/233 (39.91%), Query Frame = 0

Query: 178 IGVLSCP--QKGDTSVE-NCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQ-- 237
           +G+L  P      T V+  C IC E+     + ++ C H +C  C   Y+  K+E     
Sbjct: 102 VGILKEPVVDVNGTEVDIQCGICFESYTRKEIASVSCGHPYCKTCWTGYITTKIEDGPGC 161

Query: 238 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDK--IYCPYPNCSVLLDP 297
           + ++CP+  C   +  +           +        + +   K   +CP P C      
Sbjct: 162 LRVKCPEPSCYAVVGQDMIDEVTEKKDKDKYYRYFLRSYVEDGKKMKWCPSPGC------ 221

Query: 298 SECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITL 357
            EC +     SS  D  C+    C    C  C    HS + CE       + +D ++   
Sbjct: 222 -EC-AVEFGESSGYDVACL----CSYRFCWNCSEDAHSPVDCETVSKWIFKNQDESE--- 281

Query: 358 HRLARNKRW-----RRCQQCRRMIELAQGCYHMTC--WCGHEFCYSCGAEYQD 397
                NK W     + C +C+R IE + GC HMTC   CGH FC+ CG  Y D
Sbjct: 282 -----NKNWILANSKPCPKCKRPIEKSHGCNHMTCSASCGHRFCWICGKSYSD 314

BLAST of Carg25412 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 8.0e-14
Identity = 61/244 (25.00%), Postives = 97/244 (39.75%), Query Frame = 0

Query: 193 NCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQ--VPIRCPQLRCKYYITSNE 252
           +C IC E+ P     AL C H++C  C K+Y++ K+      +   CP  +CK  +  + 
Sbjct: 138 SCLICLEDYPPTQTFALICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQDA 197

Query: 253 FKSFLPLTLYESLENTLAEANIHSDK--IYCPYPNCSVLLDPSECLSARASSSSQSDNTC 312
           FK  +   ++E   N + ++ +  +    +CP P C          S R     + +   
Sbjct: 198 FKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGC--------IYSIRCDRKERKEAVN 257

Query: 313 IECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRM 372
            +C     F C +  +  H    C +      +  D ++     LA  K   +C +CR  
Sbjct: 258 CKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCPECRSP 317

Query: 373 IELAQGCYHMTC-----WCGHEFCYSCGAEYQD------GQQTCQCVFWDDDDNDDDNSE 422
           IE   GC HMTC      CG EFC+ C   + +      G   C          DDD + 
Sbjct: 318 IEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYYNCNKYDKSKAKEDDDKAH 370

BLAST of Carg25412 vs. ExPASy TrEMBL
Match: A0A6J1K3V2 (probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita maxima OX=3661 GN=LOC111490412 PE=3 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.2e-299
Identity = 504/512 (98.44%), Postives = 507/512 (99.02%), Query Frame = 0

Query: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60
           ME+REMFGPAEEDDVEDFRSCCEDHEDVVEEGS  EEEEDLDEFSVKMFFKG+SIAEIGD
Sbjct: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGS--EEEEDLDEFSVKMFFKGISIAEIGD 60

Query: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120
           PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT
Sbjct: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120

Query: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180
           DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV
Sbjct: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180

Query: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240
           LSCPQKGD S+ENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL
Sbjct: 181 LSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240

Query: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300
           RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS
Sbjct: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300

Query: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360
           SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR
Sbjct: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360

Query: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV 420
           RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQC FWDDDD DDDNSEDLV
Sbjct: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD-DDDNSEDLV 420

Query: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480
           THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480

Query: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 509

BLAST of Carg25412 vs. ExPASy TrEMBL
Match: A0A6J1GTW7 (probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita moschata OX=3662 GN=LOC111456904 PE=3 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.1e-299
Identity = 505/514 (98.25%), Postives = 506/514 (98.44%), Query Frame = 0

Query: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60
           MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGS  EEEEDLDEFSVKMFFKGVSIAE+GD
Sbjct: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGS--EEEEDLDEFSVKMFFKGVSIAEVGD 60

Query: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120
           PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT
Sbjct: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120

Query: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180
           DSNTLYNQITNEEEPEIPLLVALKERILEH STFEAFSLNLSSHSDLEQPFHLAKVAIGV
Sbjct: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHTSTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180

Query: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240
           LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL
Sbjct: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240

Query: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300
           RCKYYITSNEFKSFLPLTLYE LENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS
Sbjct: 241 RCKYYITSNEFKSFLPLTLYELLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300

Query: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360
           SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR
Sbjct: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360

Query: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDN--DDDNSED 420
           RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQD QQTCQC FWDDDDN  DDDNSED
Sbjct: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDEQQTCQCAFWDDDDNDDDDDNSED 420

Query: 421 LVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRC 480
           LVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRC
Sbjct: 421 LVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRC 480

Query: 481 TDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           TDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 TDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 512

BLAST of Carg25412 vs. ExPASy TrEMBL
Match: A0A1S3C9E4 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.2e-268
Identity = 450/520 (86.54%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 1   MENREMF------GPAEEDDVEDFRSCCEDHE--DVVEEGSEEEEEEDLDEFSVKMFFKG 60
           ME+R++F      GPAEEDDVE+FRSCCEDHE     E+  EEE EEDLDEFSVKMFFKG
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60

Query: 61  VSIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNG 120
           VSIAEIGDP+SGLSGIGVFME+SGK+PF+QVQKKLDFYVDELVADYLALMDGLVEA+QN 
Sbjct: 61  VSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120

Query: 121 VRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFH 180
           VR IRAFTDS+ LY QITNEEEPEIPLLVALKERILEHAS FE+FSL LSS +DLEQPFH
Sbjct: 121 VRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQPFH 180

Query: 181 LAKVAIGVLSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQ 240
           LAKVAIGVLSCPQKGD SVENCSICC+NKP+AMMVALKCCHKFCS C+K+YVDGK++ SQ
Sbjct: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDASQ 240

Query: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSE 300
           VPIRCPQLRCKYYITSNEFKSFLPLTLYE LENTLAEANIH+D+IYCP+PNCSVLLDPSE
Sbjct: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDPSE 300

Query: 301 CLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHR 360
           CLSARASSSSQSDN+CIECPVCQRFICVEC VPWHSS+SCEEFQ+IPLEERD ADITLHR
Sbjct: 301 CLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360

Query: 361 LARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDND 420
           LA+NKRWRRCQ+C RMIEL QGC+HMTCWCGHEFCYSCG EYQDGQQTCQC FW     D
Sbjct: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFW-----D 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           +DNSE+LVTHS+QESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSDHQPY
Sbjct: 421 EDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           QSPPRCTDSYVDTMKD+HQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of Carg25412 vs. ExPASy TrEMBL
Match: A0A5D3E1X1 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G001170 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.2e-268
Identity = 450/520 (86.54%), Postives = 483/520 (92.88%), Query Frame = 0

Query: 1   MENREMF------GPAEEDDVEDFRSCCEDHE--DVVEEGSEEEEEEDLDEFSVKMFFKG 60
           ME+R++F      GPAEEDDVE+FRSCCEDHE     E+  EEE EEDLDEFSVKMFFKG
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEESEEDLDEFSVKMFFKG 60

Query: 61  VSIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNG 120
           VSIAEIGDP+SGLSGIGVFME+SGK+PF+QVQKKLDFYVDELVADYLALMDGLVEA+QN 
Sbjct: 61  VSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQND 120

Query: 121 VRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFH 180
           VR IRAFTDS+ LY QITNEEEPEIPLLVALKERILEHAS FE+FSL LSS +DLEQPFH
Sbjct: 121 VRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQPFH 180

Query: 181 LAKVAIGVLSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQ 240
           LAKVAIGVLSCPQKGD SVENCSICC+NKP+AMMVALKCCHKFCS C+K+YVDGK++ SQ
Sbjct: 181 LAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDASQ 240

Query: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSE 300
           VPIRCPQLRCKYYITSNEFKSFLPLTLYE LENTLAEANIH+D+IYCP+PNCSVLLDPSE
Sbjct: 241 VPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDPSE 300

Query: 301 CLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHR 360
           CLSARASSSSQSDN+CIECPVCQRFICVEC VPWHSS+SCEEFQ+IPLEERD ADITLHR
Sbjct: 301 CLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITLHR 360

Query: 361 LARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDND 420
           LA+NKRWRRCQ+C RMIEL QGC+HMTCWCGHEFCYSCG EYQDGQQTCQC FW     D
Sbjct: 361 LAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFW-----D 420

Query: 421 DDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480
           +DNSE+LVTHS+QESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSDHQPY
Sbjct: 421 EDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPY 480

Query: 481 QSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           QSPPRCTDSYVDTMKD+HQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 QSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of Carg25412 vs. ExPASy TrEMBL
Match: A0A6J1CLL9 (uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012584 PE=3 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 8.8e-266
Identity = 454/524 (86.64%), Postives = 481/524 (91.79%), Query Frame = 0

Query: 1   MENREMF------GPAEEDDVEDFRSCCEDH------EDVVEEGSEEEEEEDLDEFSVKM 60
           ME+RE+F      GPAEEDDVEDFRSCCEDH      ED+VEEGSEEEE   LDEFSVKM
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKEAEDLVEEGSEEEEH--LDEFSVKM 60

Query: 61  FFKGVSIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEA 120
           FFKG+SIAEIGDP+SGLSGIGVFME+SGKVPFIQVQKKLDFYVDELVADYLALMDGLVEA
Sbjct: 61  FFKGISIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEA 120

Query: 121 IQNGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLE 180
           +QN +R IRAFTDS+ LYNQITNEEE EIPLL+ALKERILEHASTFEAFSLNLSS +DLE
Sbjct: 121 MQNEIRCIRAFTDSDLLYNQITNEEESEIPLLIALKERILEHASTFEAFSLNLSSSTDLE 180

Query: 181 QPFHLAKVAIGVLSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKL 240
           QPFHLAKVAIGVLSCPQKGD SVENCSICCENKP+AMMV LKCCHKFCSHCMK+YVDGK+
Sbjct: 181 QPFHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVGLKCCHKFCSHCMKTYVDGKV 240

Query: 241 ETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLL 300
           ++SQVPIRCPQLRCKYYIT NEF+SFLPLTLYESLENTLAEANIHSD+IYCP+PNCSVLL
Sbjct: 241 QSSQVPIRCPQLRCKYYITINEFRSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLL 300

Query: 301 DPSECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADI 360
           DPSECLS RASSSSQSDN+CIECPVCQRFICV C VPWHSSMSCEEFQ+I     D  DI
Sbjct: 301 DPSECLSGRASSSSQSDNSCIECPVCQRFICVYCGVPWHSSMSCEEFQNI-----DTEDI 360

Query: 361 TLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDD 420
           TLHRLA+NKRW+RCQQCRRMIEL QGCYHMTCWCGHEFCYSCGAEYQD QQTCQC  W  
Sbjct: 361 TLHRLAQNKRWKRCQQCRRMIELTQGCYHMTCWCGHEFCYSCGAEYQDSQQTCQCALW-- 420

Query: 421 DDNDDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSD 480
              D++NSEDLVTHSIQESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSD
Sbjct: 421 ---DEENSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSD 480

Query: 481 HQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 513
           HQP+QSPPRCTDSYVDTMKDLHQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 HQPHQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 512

BLAST of Carg25412 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 653.7 bits (1685), Expect = 1.2e-187
Identity = 341/535 (63.74%), Postives = 416/535 (77.76%), Query Frame = 0

Query: 1   MENREMFGPAEE------DDVEDFRSCCEDHE------DVVEEGSEEEEEEDL-DEFSVK 60
           MEN+E     EE       D E+FRSCC D E      D + +  EEE+++DL DEFSVK
Sbjct: 1   MENQEDSCECEELPLNHLGDEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVK 60

Query: 61  MFFKGVSIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVE 120
           M FKGVSI E GD  SG SGIGV +ERSG +  IQVQKKLDFYV+E VA+YLALMDGL  
Sbjct: 61  MVFKGVSIFERGDLGSGYSGIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEV 120

Query: 121 AIQNGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDL 180
           A+QN + S+ A TDS  LYNQIT EE+ EIPLLVAL+ER+LE  S    F L L+   DL
Sbjct: 121 ALQNNLSSVVAVTDSELLYNQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGK 240
           ++   LA+VA+G++S    GD  +ENCSICCE++ + MM++LKC HKFCSHCMK+YV+GK
Sbjct: 181 DEALSLAQVAVGIVSSNLDGDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGK 240

Query: 241 LETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANI---HSDKIYCPYPNC 300
           ++TS+VPIRCPQ++CK+Y+++ E KSFLP+T ++S E    EAN+   ++ KIYCPYPNC
Sbjct: 241 VKTSEVPIRCPQVQCKHYLSAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNC 300

Query: 301 SVLLDPSECLS---ARASSSSQSDNT-CIECPVCQRFICVECCVPWHSSMSCEEFQDIPL 360
           S LLDP ECLS   A +SSS+QS+N+ C+ECPVC+RF+CV+C VPWH+SMSCEEFQ +P+
Sbjct: 301 SFLLDPQECLSSGRASSSSSTQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPV 360

Query: 361 EERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQT 420
           +ER   DITLHRLAR KRWRRCQQCR MIELAQGC HMTC CGHEFCYSCGAEY++GQQT
Sbjct: 361 DERYPDDITLHRLARYKRWRRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQT 420

Query: 421 CQCVFWDDDDNDDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFL 480
           C C FWDDD+ + +N+ +   ++IQE EQW W+TFNS+P + DAYSEQERSQLALIQRFL
Sbjct: 421 CTCAFWDDDEEEQENAVE--ENTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFL 480

Query: 481 A-GGFSLSDHQ-PYQS-PPRCTD-SYVD-TMKDLHQLPWLERFVSVISDNYYEDY 511
           A GGFSLSDH   YQS PP CT+ SYV+  MKDLHQLPWLERFVSVISD+YYE+Y
Sbjct: 481 AGGGFSLSDHHTTYQSPPPPCTESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

BLAST of Carg25412 vs. TAIR 10
Match: AT4G19670.2 (RING/U-box superfamily protein )

HSP 1 Score: 653.7 bits (1685), Expect = 1.2e-187
Identity = 341/535 (63.74%), Postives = 416/535 (77.76%), Query Frame = 0

Query: 1   MENREMFGPAEE------DDVEDFRSCCEDHE------DVVEEGSEEEEEEDL-DEFSVK 60
           MEN+E     EE       D E+FRSCC D E      D + +  EEE+++DL DEFSVK
Sbjct: 1   MENQEDSCECEELPLNHLGDEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVK 60

Query: 61  MFFKGVSIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVE 120
           M FKGVSI E GD  SG SGIGV +ERSG +  IQVQKKLDFYV+E VA+YLALMDGL  
Sbjct: 61  MVFKGVSIFERGDLGSGYSGIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEV 120

Query: 121 AIQNGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDL 180
           A+QN + S+ A TDS  LYNQIT EE+ EIPLLVAL+ER+LE  S    F L L+   DL
Sbjct: 121 ALQNNLSSVVAVTDSELLYNQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDL 180

Query: 181 EQPFHLAKVAIGVLSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGK 240
           ++   LA+VA+G++S    GD  +ENCSICCE++ + MM++LKC HKFCSHCMK+YV+GK
Sbjct: 181 DEALSLAQVAVGIVSSNLDGDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGK 240

Query: 241 LETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANI---HSDKIYCPYPNC 300
           ++TS+VPIRCPQ++CK+Y+++ E KSFLP+T ++S E    EAN+   ++ KIYCPYPNC
Sbjct: 241 VKTSEVPIRCPQVQCKHYLSAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNC 300

Query: 301 SVLLDPSECLS---ARASSSSQSDNT-CIECPVCQRFICVECCVPWHSSMSCEEFQDIPL 360
           S LLDP ECLS   A +SSS+QS+N+ C+ECPVC+RF+CV+C VPWH+SMSCEEFQ +P+
Sbjct: 301 SFLLDPQECLSSGRASSSSSTQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPV 360

Query: 361 EERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQT 420
           +ER   DITLHRLAR KRWRRCQQCR MIELAQGC HMTC CGHEFCYSCGAEY++GQQT
Sbjct: 361 DERYPDDITLHRLARYKRWRRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQT 420

Query: 421 CQCVFWDDDDNDDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFL 480
           C C FWDDD+ + +N+ +   ++IQE EQW W+TFNS+P + DAYSEQERSQLALIQRFL
Sbjct: 421 CTCAFWDDDEEEQENAVE--ENTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFL 480

Query: 481 A-GGFSLSDHQ-PYQS-PPRCTD-SYVD-TMKDLHQLPWLERFVSVISDNYYEDY 511
           A GGFSLSDH   YQS PP CT+ SYV+  MKDLHQLPWLERFVSVISD+YYE+Y
Sbjct: 481 AGGGFSLSDHHTTYQSPPPPCTESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

BLAST of Carg25412 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 184.5 bits (467), Expect = 2.1e-46
Identity = 118/379 (31.13%), Postives = 200/379 (52.77%), Query Frame = 0

Query: 47  KMFFKGVSIAE--IGDPSSGLSGIGV-FMERSGKVPFIQVQKKLDFYVDELVADYLALMD 106
           +++FKG+   E   G  +  +SG GV   ++   + F      +D  +    A+  AL+ 
Sbjct: 146 RLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDNLLFEMKGPLIDNGMSRQGAELKALIR 205

Query: 107 GLVEAIQNGVRSIRAFTDSNTLYNQITNE---EEPEIPLLVALKERILEHASTFEAFSLN 166
           GL EA++ G++ I  F DS  ++  +T +   ++ +I LL+   + I++H S+++     
Sbjct: 206 GLTEALKLGIKHIVFFCDSYPIFQYVTGKWMAKQKKISLLLDDLQSIMQHFSSYQHV--- 265

Query: 167 LSSHSDLEQPFHLAKVAIGVLSCPQKGDTSV-----ENCSICCENKPTAMMVAL-KCCHK 226
           L + +D++  + LA+ +I  +  P +          E C+IC  +     M ++ KC H+
Sbjct: 266 LVARNDVKFAYKLARESILSMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCRHR 325

Query: 227 FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIH- 286
           FC  C+K +V+ KL     P +CP   CK  +  +     L   L +  +  L E  I  
Sbjct: 326 FCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIPV 385

Query: 287 SDKIYCPYPNCSVLLDPSECL-SARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSC 346
           ++++YCPYP CS L+  ++   SA++  S    +    C  C+   CV+C VPWH ++SC
Sbjct: 386 TERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSC 445

Query: 347 EEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGA 406
            E++ +   E    D+ L  LA NK WR+C +C+ MIEL+QGC H+TC CGHEFCY+CG 
Sbjct: 446 TEYKKLH-PEPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNCGG 505

Query: 407 EYQDGQQTC--QCVFWDDD 410
            +     TC  +C  W+++
Sbjct: 506 GWNKIMGTCLNRCPTWNEE 519

BLAST of Carg25412 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 175.6 bits (444), Expect = 1.0e-43
Identity = 112/327 (34.25%), Postives = 178/327 (54.43%), Query Frame = 0

Query: 99  LALMDGLVEAIQNGVRSIRAFTDSNTLYNQIT--NEEEPEIPLLVALKERILEHASTFEA 158
           +AL+ GL E+   G+R +  + D   LY  I    + + ++  LV   + +LE  +  +A
Sbjct: 1   MALIRGLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIKKKVVHLVDEFQLLLEEMTYTDA 60

Query: 159 FSLNLSSHSDLEQPFHLAKVAIGVLSCPQKGDTSVENCSICCENKPTAMM--VALKCCHK 218
              +L + +D+   F LA+ AI       + D   E CSIC E      M     KC H+
Sbjct: 61  ---DLIALNDVNFAFKLAREAI-----VSRDDVKAEICSICFEETEGERMFFTTEKCVHR 120

Query: 219 FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANI-H 278
            C  C+K YV+ KL +  VP  C    CK+ +T       L L L E  +  + E +I  
Sbjct: 121 HCFPCVKQYVEVKLLSGTVP-TCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPA 180

Query: 279 SDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCE 338
           +++IYCPYPNCS+L+  +E  S    S   +D +C++C  C  F C++C VP HS +SC 
Sbjct: 181 AERIYCPYPNCSMLMSKTELSS---ESDLSNDRSCVKC--CGLF-CIDCKVPSHSDLSCA 240

Query: 339 EFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAE 398
           E++ +   +  + ++ L  LA++K+WR+C+ CR MIEL+  C HMTC CG++FCY C  E
Sbjct: 241 EYKKLH-HDPLVDELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQCEVE 300

Query: 399 YQDGQQTCQ--CV-----FWDDDDNDD 414
           +++ Q+TC   C+     ++DD D ++
Sbjct: 301 WKNDQKTCSSGCLLTGHGYYDDYDYNE 311

BLAST of Carg25412 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 173.7 bits (439), Expect = 3.8e-43
Identity = 119/363 (32.78%), Postives = 192/363 (52.89%), Query Frame = 0

Query: 41  LDEFSVKMFFKG-VSIAEIGD--PSSGLSGIGVFMERSGKVPFIQVQKKL-DFYVDELVA 100
           +D    ++ FKG V+   + D    +  +G GV +         ++++ L D  +     
Sbjct: 149 MDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIKESLRDIEISRRGV 208

Query: 101 DYLALMDGLVEAIQNGVRSIRAFTDSNTLYNQI--TNEEEPEIPLLVALKERILEHASTF 160
           + +AL+ GL E+   G+R++  + D + +Y  I    + + +I  LV   + ILE  +  
Sbjct: 209 EIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSKKKIDHLVEEVQGILEKMACI 268

Query: 161 EAFSLNLSSHSDLEQPFHLAKVAIGVLSCPQKGDTSVENCSICCENKPTA-MMVALKCCH 220
           +A    L + +D++  F LA+ AIG  S     +   E C I  E      M V  KC H
Sbjct: 269 DAV---LVARNDVKFAFRLAREAIGRNSVDVNAEQG-ETCGIFFEETDVEHMFVTEKCLH 328

Query: 221 KFCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANI- 280
           + C  C+K +V  KL +   P  C +  CK+ +T       L L L E  +  + E +I 
Sbjct: 329 RHCFPCVKQHVKVKLRSGTEP-TCLEYGCKFKLTLERCSKVLTLKLIEMWKQKMKEDSIP 388

Query: 281 HSDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSC 340
            +++IYCPYPNCS+L+  +E LS+ A  S+    TC++C  C  F C++C VP H+ +S 
Sbjct: 389 AAERIYCPYPNCSMLMSKTE-LSSEADLSNV--RTCVKC--CGLF-CIDCKVPSHTDLSY 448

Query: 341 EEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGA 396
           ++++ +  +   + D+ L  LA +K WR+C +CR MIEL+ GC HMTC CG+EFCY CG 
Sbjct: 449 DDYKKLHPDPL-VDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCGYEFCYECGI 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012597.11.4e-305100.00putative E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma... [more]
KAG6573449.18.4e-30399.41putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022994789.12.5e-29998.44probable E3 ubiquitin-protein ligase ARI11 [Cucurbita maxima][more]
XP_022954734.14.3e-29998.25probable E3 ubiquitin-protein ligase ARI11 [Cucurbita moschata][more]
XP_023541881.13.8e-29597.27probable E3 ubiquitin-protein ligase ARI10 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8TC411.2e-1729.90Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE... [more]
D3YYI71.2e-1729.90Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P... [more]
Q9SKC31.9e-1525.70Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q9SKC47.2e-1526.18Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=AR... [more]
Q6T4868.0e-1425.00Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G... [more]
Match NameE-valueIdentityDescription
A0A6J1K3V21.2e-29998.44probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GTW72.1e-29998.25probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A1S3C9E44.2e-26886.54E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 S... [more]
A0A5D3E1X14.2e-26886.54E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1CLL98.8e-26686.64uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
Match NameE-valueIdentityDescription
AT4G19670.11.2e-18763.74RING/U-box superfamily protein [more]
AT4G19670.21.2e-18763.74RING/U-box superfamily protein [more]
AT5G60250.12.1e-4631.13zinc finger (C3HC4-type RING finger) family protein [more]
AT2G25360.11.0e-4334.25RING/U-box superfamily protein [more]
AT2G25370.13.8e-4332.78RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 342..401
e-value: 0.087
score: 13.6
coord: 261..332
e-value: 7.0E-9
score: 45.5
IPR002867IBR domainPFAMPF01485IBRcoord: 353..401
e-value: 6.8E-7
score: 29.4
coord: 270..332
e-value: 6.7E-8
score: 32.6
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 362..403
e-value: 11.0
score: 2.3
coord: 194..240
e-value: 0.0039
score: 26.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 194..241
score: 10.792683
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 187..272
e-value: 4.0E-14
score: 54.3
NoneNo IPR availableGENE3D1.20.120.1750coord: 330..461
e-value: 2.4E-14
score: 55.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availablePANTHERPTHR11685:SF353RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 13..511
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 190..270
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 345..408
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 46..180
e-value: 7.0E-8
score: 34.5
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 64..161
e-value: 3.4E-10
score: 39.8
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 13..511
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 211..220
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 190..407
score: 29.013382
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 62..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25412-RACarg25412-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0004386 helicase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity