Carg25318 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25318
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAcetyl-coenzyme A synthetase
LocationCarg_Chr11: 10387993 .. 10397686 (-)
RNA-Seq ExpressionCarg25318
SyntenyCarg25318
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATTTTCTTCATCCCTTCCGCTACCATTGTCAATAGTTCTTCTTCTTTATTACTTCCTCTGTCAGAATCAGGGTCGACAAGGCGATGGCCGACATGGGTTTCCGGAATGTGATAGCATCCATGGATCCATTGGCTACCTCCCGTAAGATACCTCTGCTCAATGCCACTGCCATTGGGGAGTCTCCTGATACCCAAGATGATCATCTCATTATACCGGATCATCTATTCTCTTCCCGTGCTCGTATTTCCTCCCACGATCAGGTTCATTTCTCTTAATTTCATTATCATTGATTCGTTTTTGTGCATGATCTCATCTGGGTTTGGTTTGCTTGTTTTGAATCTATTACTCTGTTAGTATTTGGAGATGTACAATAGGTCCATTGATGACCCTTCTGGATTTTGGTCCGATATTGCGGCTTCTGAGTTCTTTTGGAAACAGAAATGGGATCAGAAAGTCTACTCTGAGAATCTTGATGTCCGGAAAGGTGAAATCAAGATAGAGGTGAGCCTCAATTCTCATGATCTTTGCTTTCAGGCTTGTTTAGTGTGAGATCCACTTGGTTGGGAAGGAGAACGAAACCTTCTTTATAAGGGCATGGAAATCTCTTCCTAGTACTAGATGTGAAACGTTGAGGAAAAGCTTACCGAGGACAATATCTACTAGCAATGGGCTTGAGCGGTTACAAATGGTATTAGAGCCAGACACTGAGCGATGTGTCAATGAGGAAGTTGAGCCCCGAATGGGGTGGACACGAGACGGTGTGCCAGCAAGGACTTTAGGCCTTGTAGGGGTGAATTGTGAGATCCCACGTTGGTTGGGGAGGAGAACGAAACATCCTTTATAAGGGTGTGGAAACCTCTCATTAATAGACAAAAAAGACAAGATCTACTAGCGGTGGGCTTGGGCGGTTACATATATTCCTGGTTTCGTTTACCTTTCTTTTATCATTAATTAGAACAAAAAGATCATTCATTTCTCTTTCTCCTTCTAATACTTCTCCATCCCGACCAGTGGTTCAAAGGTGGAATCACCAACATTTGTTACAACTGTCTTGATAGAAACATTGAAGCTGGACTTGGGGAAAAGGTTGCTCTCTACTGGGAAGGAAACGAGCCTGGTTTTGATAGCTCCTTAACTTACACTCACCTTCTAAACAAAGTTTGTCAGGTTATGGTAAACTCCTTGTTTCTTCATAAACTCGAACAACCCACAAATTTTAATGCCTCTTTTGGTCTGACTTTGAGCTTATCCATCTGCCAAAGCTAGCAAGCTTCTTGAAAGCTAATGGAGTCAGGAAAGGTGATGCTGTTGTGATCTATATGCCCATGCTAATGGAGCTTCCAATCGCCATGCTTGCCTGTGCTAGAATTGGTGCTATACATTCGGTACTCATCAACTTTACTCGTCCACCTACCTCGAATTCTCTTTGATTTTCTTTATTATTTCAGGTCGTTTTTGGGGGGTTTTCTTCAGAATCTCTTGCTCAAAGAATCATAGATTGTAAACCAAAAATTGTGATTACTTGCAATGCTGTTAAAAGGGGTTCTAAAGTTATCCATCTTAAAGACATTGTGGATGTTGCTCTCACAGATTCAGCACAAAATGGAGTCTCTGTAGGTGGGAACTTTGGAAGTTTACTGTTGTTATAATTGTACTTTCTGCATGTGTTTGCTTTGTGTTTATGAACCTTTGCTATGATATTTCTTTGTCGAAATTGTAATTTTCTGTTCGTATTCAGCTTTGTTTTGTAGGCATTAGAATCATTGTAACCTTGACAAGGACATTCTGTAAGATTTCATATTGGTTGGGGAGGAGAACGAGACATGCTTTACAAGGGTGTGGAAACCTCTCCCTAGTAGATGTGTTTTAAAAACGTTGAGGGGAAGCCCGAAAGGGAAAGTCCAGAAAGGATAATATCTGCTAGCAGTGGGCTTGGGCTATTACAAATGGTATCATAGTCAGACACTGGACGATGTGCCAGCGAGGAGGCTGAGCCCTGAAAGGGAGTGGACATGAGGCGGTGTGCCAGAAAGGACGTTCGGCCTTGGGGGTGGTTGGGGCTCCCACATCAATTGGAGAAGGGAACGAGTGCCAGCGAGGACGCTGAGCCTCAAAAGGGGGTGGATTGTGATATCTCACATCAGTTGGGGAGGAGAACGAAACATTCTTTATGAGGATGTGGAAACCTCTCCCTAGTAGATGCATTTTAAAAACCTTGAGGGGAAGCCCGAAAGGAAAAACCCAAAGAGGATAATATCTGCTAGTGGTGGGCTTGGGTTGTTACAATGGTATCCGAGCCAGACACTAAGCGATGTTCTAGCGAGGAGACTGAGCTCCGAAAGGGGATGGACATGAGGCGGTGTGCCAAAAAGGATGCTGGACCTTGAAGGGGGGTGATTGGAGTCCCACATCGATTGGAGAAGGAAACGAGTGCCAACGAGGACATTGGGCCTCGAAGGGGGATGGATTCTAAAATCTCACATCGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGGGGAAACCTCTCCCTAGTAGACGCGTTTTAAAAACCTCAACGGGAAGCCCGAAAGTGAAAACCCAAAGAAGACAATATCTACTAACAGTGGGCTTAGGCCGTTACACATTTCTTCCTTGTAACCTCTTTCATCTCTTCCTGCAGCAACGTGCCTTACGTACGAGAACGAGTCAGCGCTAAAGAGAGAATCCACTAAATGGAAGGAAGGGAGGGATATATGGTGGCAGGTTTGCATATCCTACTTCATGCTAATTCAAATTATGTTCTGTTCAATGCCCCTTGTTTCTTGTTGATATATGTATTTGCTGCATGCTATCAACATGTATAATCAAATGTATATTTATACCGCTAATGATGAAATATACAAAAAAGGGCCTCGAGGTATCTCGGAACCCTTCCTCCCTCACAAGACAAGGGAAACCTGTAGTTACCGATCTACTTGGTATTCTCGACGTATGTGGGGAGACCTCTTAAAGCAACTAGCGAGAATGGCTAGGTTGATAGCATAGGGTTAAAGAAAAGACGAATATGAAAAGATGTATCGCGTAACCGTCCCAATCTGGAAACGAACTGAATGCTGTACTCGATTGGTTGTAATGTCTGCAGGATGCTGTCTCAACATATCCAACCACATGTACAGTGGAATGGGTTGATGCAGAGGATCCACTTTTCCTGCTTTATACTAGCGGGAGTACAGGAAAGCCAAAGGTAAACCTTTTCCATCATTTAGAGCTGCTTTTCTACTTTGACCCGAACAATCATAGTTCGAGTTTTGTAGGGTGTTCTTCATACAACTGGAGGATACATGGTGTATACAGCAACGACATTCAAGTATGCATTTGATTACAAACTGACCGATGTGTACTGGTATTTTCGAAACTTAGATCAACTGTAACGTGCTTTGACGTACATTTATATTTACGGTTTCACGACGTATCGTTCGCCTGATTTTAGGTGTACAGCTGACTGTGGTTGGATCACTGGGCACAGTTATGTCACGTATGGACCTTTACTCAATGGAGCTAGTGTTGTTGTTTTTGAAGGGGTAATGATTGACCATTTCATTTTTCTTTTTCCTTTGATGTTTGCAATTTGTGTCTAACGAGTGTCGAAGTGTTTGTCGGATATAGACACGCTAGCCAAACTAAAGTGTATGTGCTTTTTAAAATGAATGTAAGACAACCTCGGTTCGTGCGAGATGGCAGAGCCATTCTGGGCTAAGGTTTGGTTCCATGGTGGGCTAGGCTTTGGAGAGTACTGTGAATTTCTTTATGTCATGGCTCAAGCCCACCGCTAGCAGATATTGTCCTCTTTATACGTTCCCTTTCAGGCTTCCCCTCAAAGTTTTTAAAATGCGTCTGCTAGGGAGAACTTTTCACACCCTTATAAAGGGTGTTCCATTCTCCTCCCAACCGATGTGGGATCTCACAATCCACTCCCCTTCAGGGCCCAGCATCCTTGTTGGCACTCGTTCCTTTCTCCAATCGATGTGAGACCCCACCAAATCCACCCACCTTCGGGGCCCAGTGTCCTTACTGGCACACCACATCGTGTTTACCCTCTTCGGGGAACAGCCTCCTCGCTGGCACATCGCCTAGTGTCTGGCTTTGATACCATTTGTAACGGCCCAAGCCCACCGCTAGCAGATATTGTCCTCTTTTTTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAGGGTATTTCGTTCTCCTCCCCAACCGACGTGGGATATCCCAAGTAAAAATAATGCTCAGTTTTGGTCGGAGTGTTAGGAGTCATTATATGTTGTATGCTTGTTTCTACTTGCTAACATTACATATGTTCTGTTTGCACCTACAAAATGATTCGAAGTTTTGACAAAAAACTGTCTTTCCTGCTGCAGGCCCCAAATTATCCCGATCCTGGACGTTGTTGGGACATAGTTGATAAGTATAAGGTGACAATATTCTATACCGCTCCAACATTAGTACGGTCCCTCATGCGTGAAGGGAACGAGGTACGTCGTGGTTCTAATCTTATATTCCCAAATATTGTTGAGTCGACGACATTATAAGAACATCTAACGTGTGATTTCGCGGAGGTGAATATCAATCCTTTTTAGTATTTTCCATTTTTCGGATATTTCGAAAGTTAAAAGTTGAAAGTACTTCAGAAAATATACTTGGGAGATGAGGATGTAACGGCAGAAGCCCCCGTTAGCAAATATTGTCCTCTTTGGGTTTTCTCTTTTGGGCTTCCCCTCAAAGTTTTTAAAACGCGTCTACTAGAGAGAAGTTTCCACACTCTTATAAAGAATACTTTGTTCTCCAATGCAATCGATATGGGATCTCACAATCGATATGGGATCTCACAATCGATATGGGATCTCTTCTCCGATTGATGTGGGACCCTCCAATTCACCCCCCTTCGGGGCCTGGCGTCCTTGCTGGCACACCACCTCGTGTCCACCCCCTTCGGGGTCAGCCTCCTCACTGGCACATCGCCCGATGTCTAACTCTGATACCATTTGTAACGGCCAAGCCCACCGCTAGCAGAAATTGTTCACTTTGGGTTTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAATATGTCTGATAGGGAGAGGTTTCCACACCCTTATTAAGAATACTTCGTTCTCCTCCTCAACCGATGTGGGATCTCACAATCCAATTGGGAGGTCCCACATCGATTGGAGAAGAGAACAAGTGCCAGCAAGGATGCTGGGCTCCAAAGGGGGGTGGATTGTGAGATCCCACATCGGTTGAGGAAGAGAACGAGACATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGCAGACGCGTTTTAAAAACCTTGAGGAGAAGCCTGAAAGGAAAAGCCCAAAGAGGACAATATCTGCCATCAGTAGGCTTGAGCCGTTACACGATGTGAGGAAATATTTGTCTTAAATGCATCTCTATAATCTTTGATCACTTAGGTTACTTTTTTTTTGTCTTATATTCTTTCTTTCCCAATAACATTTAGCTTTTATTATGGCTAAGCTTTGAGTTCTTATCTTCCTACGCCCCTATGAGACTTTGCTCATGATCTTCATTAAATATTACATAAATAGTATGCTAACTACTCTAGAAAATCCTCTTCCTGCAGCATGTTACTCGCTACTCCCGAAAATCCTTACGGGTTCTTGGAAGCGTGGGCGAGCCAATCAATCCCAGTGCATGGAGGTTTGACTACTACGATGTCTCGGGATCTTGAGTCATTTTCTCTCCTTGACTTATATAAGTAGAGCGTTCTTCATTTAAAGGCTTTGAATATCATGAACTTGTCTAAATCAAACTCTGATTGTTGATTCACGAATTATCTTAATATTTTAGCCTTGAAGACAACTTTTGTTTCGTTTCGATTAAAAGATGCGTAAAATGCTCGTTGATTTCTTGTAGGTGGTTCTTCGATGTCGTTGGAGAGTCGAGGTGTCCTATATCGGACACATGGTGGCAAACTGAAACTGGTGGTTTCATGGTATATATTTCTTTTACCATAAGAAATGGCTTATGGTATTATATTTTGATAAGCCTTTAGGTTAATAATTATTCCATTGATGTTTACATTTAAATATTTACAACTAATATTTTAATATCAGTTTCACATATTTATGTTAAGTTTATCTGCAAAGAATAATTACTACACAGAGAGTTCCATTTGTAAGAGCTGAACCTCACCGTTAACTAATATTGTCCTATTTGGGATTTTCTTTCTGGGCTTTCTCTCAAGGTTTTTAAAACGTGTCTGCTAGGGGAGGTTTCCACACCCTTACAAAGAATGTTTCGTTCCCTCTCCAACCGACGTGGGATCTCACTACCCTTATAAGGGTGTGGAGAGGAGAATGAATCATTTCTTATAAGGGTGTGGAAACCTCTCTGTAGCAGACGTGTTTTAAAACCTTGAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGTTATGGGCTTGAGCGGTTACAAATGGTATCAAAGCCAAACATCAGGCGATGTGCCAACGAGAACGCTGGCCCCCAAGTGAAATCCCACATTGGTTGGAGGAGTCGAAACATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGCAAACACGTTTTACTTTTTTTAGCGCAACCGTGTTCATTTCAAACAATTCTTGTGGCAAGTAACTAAGATTTTTGTCTGGCAGATAACTCCATTACCAGGCGCATGGCCTCAGAAGCCAGGCTCTGCTACTTTTCCCTTTTTTGGAGTTCAGGTATGTAAACATACACGAGCAGCTACCTTTTCAACATTTCATCCTTACAATTTACCCCCACATATGATCATTCTTGCTTCATCATGCAGCCCATTCTGGTGGATGAAAAAGGTAATGAACTTAAAGGTGAATGCAGTGGATATCTATGCTTGAAAAGCTCATGGCCTTCAGCATTTAGAACTCTTTACAATGATCAAGAAAGATATGAAACAACCTACTTCAAGCCATTCCCTGGCTATTACTTTACTGGCGATGGCTGCACCAGGTAAATCAAGGCGCTCGATCTCATGTCTGTCATGGTACCGATCATCGAACTCATTGAGTTTAAAAACTAAGTAATGTACTCTATCTTCAAGGGATAAGGATGGATACTACTGGCTTACTGGAAGAGTTGATGATGTTATCAATGTCAGGTTTGTTGGTGTTTCTTCTTTAAGCCATATAGTTAGAACTATCCATGTATCTTTGTGGTATGATCGTTATGTTTTGCAGTGGGCATCGTATTGGCACAGCAGAAGTGGAATCTGCTTTAGTTTCACATCCCCAATGTGCCGAAGCAGCTGTAGTTGGTATCGAGCACGAGGTAGGTTTGTTGATTTCGATTTGTTAGTTGGCGATGAGCATAAATGATGGACGTAACCCATTTGCTTGGTGCGTTTGAATTTTTTTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAACATTTCTTTATATGGATGTGGAAAACCTTTCCCTAGTAGACGTGTTTTAAAACTGTGAGGCTGACGGTAATACGTAACGGACAAATATTTATTAGCAGTGGGCTTGGGTTGTTACAAATGGTATCAGAGCTAGACATCGGACGGTGTGCCAGCGAGGATGTTGGACCCTAAGAGGGGTGGATTGTGAGATCCCACATCTGTTGAAGAGGGTAACGAAGCATTCCTTATAAGATCTCCCTAGTAGACGCGTTTTAAAACCGTGAAGCTGATGGTGATACGTAACCGGCCAAAGTGGACAATATCTGCTAGCGGTGAGCTTGAGCTATTACAAATGGTATTAGAGCCAGACATTGGGTGGTGTGTCAACAAGGACGTTGGGCCCCAAGGGGAGTGGATTGTGAGATCCCACATCGGCTGGAGAGGGGAATGAAACATTTTTATATGGGTGTGGAAACTTTTCCCTAGTAGACACGTTTTAAAATTGTGAGGCTGACGGCGATACGTAATGGGCCAAAGCGGACAATATTTGTTAGCAGTTGATTTGGGCTGTTACAAATGGTATCAGAGCCAGACACCGGCCAGTGTGCTAGAGAGGACGTTGGGCCCAAGGGGAGTGGATTGCGAGATCCCACATCAGTTGGAGAGGGTAACGAAACATTCCTTGTAACAGTGTGAAAACCTCTCCTTAGTAGACGCATTTTAAAATCGTGAGGCTGATGACGATACATCACGGGCCAAAATGGAACATTTTTTTTTTATCTATTTGGTACATATGCTTTTGCCAGTATAATACATGATCATTTTATTGTGAGGTTGCAGGTGAAAGGACAGGGGATTTATGCATTTGTGACTCTAGTGGATGGCGTTTCTTATAGTGAAGAGCTAAGGAAAAGCCTTATTCTCACGGTTAGAAACCAGGTAAGTCCATTATTTGTGTACCACAAGATTTCATAACCCTTTTAACGAGTGCTCGTTGGCCCCGGAGGAGAGTGGATTGTAAGATCTCACATTAGTTGGAGACGGGAACGAAACATTCATTATAAGAGTGAAGAAACCTCTCCCTAGCTTTTAAAAACCTCGAGGTGAATCCCGTAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGCTGTGGGCTTAGGCTGTTACAAATGGTATCAGAGCCAGACATAAGACGATGTGCCAGCGAGGATGTTGAGCCTCCAAAAGAGTGGATTGTGAGATCCCACATCTTCAGAGAGGGGAACGAAACATTTTTTACAAGGGTGTGGAAACCTCTCCCTAGGAGACGCGTTTTAAAAACCTCGAGAGGAAGTCCGAAAGGGGAAACTCAAAGAGGATGACAATATCTGTTAGCGGTGGGCTTGAGCTGTTACAAATGGTATCAGAGTCAGACATCGGGCAATGTGCCAGCGAGGACGTTGGGCCTCCAAGGGGGTGGATTATGAGATCTCACATCTTCGGAGAGGGGAACGAGTGCCAGCAAAGACACTGTGCCCTGGAAGGGGGTGGATTGTGAGATCCCACATCGACTGAAGAGGGGAACGAAACATTCTTTATAAGAGTATAGAAACCTCTCTCTAGCAGATGTGTTTTAAAAACTTGAGAGGAAGCCCGAAAGGGAAGGTCCAAAAAAGATGACAATATCTACTAGCGGTGGGCCTAGGCTGTTACAAGTCGTCTCTGATCTGTTTTCAGATAGGAGCATTTGCAGCACCCGACAAAATCCATTGGGCGCCTGGACTTCCAAAAACAAGGAGCGGTAAAATAATGAGAAGAATTCTAAGGAAAATTGCGTCCATGCAGCTAGATGAACTTGGAGACACTAGCACTCTAGCAGATCCTGATGTGGTTCATCACCTCATCAGCCTTGCTGATCGCTGAATGTCACTATTACTAATGATATTAAAAAACATTCAAAGTTATACTCATCTTCAACCTACCTTATGCATATAATATTGAGTTAAGTTCATCGAAACTTGTCTTTCGTGACATCGTTACTAATTTGATATCAAGTTGAAATATTGTTTAGTTCTACTGTTCGATCGTTGACTTCTTAAATATGAAATGATTAGTTATTTCTGTACCTCGTTCTATCATGACGGATATCATACTTTGTTTGTATTTTTAAAACGTGTACTATCAGTTCTATCCATTTTTATTATCGATAAATATAATTTT

mRNA sequence

TCATTTTCTTCATCCCTTCCGCTACCATTGTCAATAGTTCTTCTTCTTTATTACTTCCTCTGTCAGAATCAGGGTCGACAAGGCGATGGCCGACATGGGTTTCCGGAATGTGATAGCATCCATGGATCCATTGGCTACCTCCCGTAAGATACCTCTGCTCAATGCCACTGCCATTGGGGAGTCTCCTGATACCCAAGATGATCATCTCATTATACCGGATCATCTATTCTCTTCCCGTGCTCGTATTTCCTCCCACGATCAGTATTTGGAGATGTACAATAGGTCCATTGATGACCCTTCTGGATTTTGGTCCGATATTGCGGCTTCTGAGTTCTTTTGGAAACAGAAATGGGATCAGAAAGTCTACTCTGAGAATCTTGATGTCCGGAAAGGTGAAATCAAGATAGAGTGGTTCAAAGGTGGAATCACCAACATTTGTTACAACTGTCTTGATAGAAACATTGAAGCTGGACTTGGGGAAAAGGTTGCTCTCTACTGGGAAGGAAACGAGCCTGGTTTTGATAGCTCCTTAACTTACACTCACCTTCTAAACAAAGTTTGTCAGCTAGCAAGCTTCTTGAAAGCTAATGGAGTCAGGAAAGGTGATGCTGTTGTGATCTATATGCCCATGCTAATGGAGCTTCCAATCGCCATGCTTGCCTGTGCTAGAATTGGTGCTATACATTCGGTCGTTTTTGGGGGGTTTTCTTCAGAATCTCTTGCTCAAAGAATCATAGATTGTAAACCAAAAATTGTGATTACTTGCAATGCTGTTAAAAGGGGTTCTAAAGTTATCCATCTTAAAGACATTGTGGATGTTGCTCTCACAGATTCAGCACAAAATGGAGTCTCTAACGAGTCAGCGCTAAAGAGAGAATCCACTAAATGGAAGGAAGGGAGGGATATATGGTGGCAGGATGCTGTCTCAACATATCCAACCACATGTACAGTGGAATGGGTTGATGCAGAGGATCCACTTTTCCTGCTTTATACTAGCGGGAGTACAGGAAAGCCAAAGGGTGTTCTTCATACAACTGGAGGATACATGGTGTATACAGCAACGACATTCAAGTATGCATTTGATTACAAACTGACCGATGTGTACTGGTGTACAGCTGACTGTGGTTGGATCACTGGGCACAGTTATGTCACGTATGGACCTTTACTCAATGGAGCTAGTGTTGTTGTTTTTGAAGGGGCCCCAAATTATCCCGATCCTGGACGTTGTTGGGACATAGTTGATAAGTATAAGGTGACAATATTCTATACCGCTCCAACATTAGTACGGTCCCTCATGCGTGAAGGGAACGAGCATGTTACTCGCTACTCCCGAAAATCCTTACGGGTTCTTGGAAGCGTGGGCGAGCCAATCAATCCCAGTGCATGGAGGTGGTTCTTCGATGTCGTTGGAGAGTCGAGGTGTCCTATATCGGACACATGGTGGCAAACTGAAACTGGTGGTTTCATGATAACTCCATTACCAGGCGCATGGCCTCAGAAGCCAGGCTCTGCTACTTTTCCCTTTTTTGGAGTTCAGCCCATTCTGGTGGATGAAAAAGGTAATGAACTTAAAGGTGAATGCAGTGGATATCTATGCTTGAAAAGCTCATGGCCTTCAGCATTTAGAACTCTTTACAATGATCAAGAAAGATATGAAACAACCTACTTCAAGCCATTCCCTGGCTATTACTTTACTGGCGATGGCTGCACCAGGGATAAGGATGGATACTACTGGCTTACTGGAAGAGTTGATGATGTTATCAATGTCAGTGGGCATCGTATTGGCACAGCAGAAGTGGAATCTGCTTTAGTTTCACATCCCCAATGTGCCGAAGCAGCTGTAGTTGGTATCGAGCACGAGGTGAAAGGACAGGGGATTTATGCATTTGTGACTCTAGTGGATGGCGTTTCTTATAGTGAAGAGCTAAGGAAAAGCCTTATTCTCACGGTTAGAAACCAGATAGGAGCATTTGCAGCACCCGACAAAATCCATTGGGCGCCTGGACTTCCAAAAACAAGGAGCGGTAAAATAATGAGAAGAATTCTAAGGAAAATTGCGTCCATGCAGCTAGATGAACTTGGAGACACTAGCACTCTAGCAGATCCTGATGTGGTTCATCACCTCATCAGCCTTGCTGATCGCTGAATGTCACTATTACTAATGATATTAAAAAACATTCAAAGTTATACTCATCTTCAACCTACCTTATGCATATAATATTGAGTTAAGTTCATCGAAACTTGTCTTTCGTGACATCGTTACTAATTTGATATCAAGTTGAAATATTGTTTAGTTCTACTGTTCGATCGTTGACTTCTTAAATATGAAATGATTAGTTATTTCTGTACCTCGTTCTATCATGACGGATATCATACTTTGTTTGTATTTTTAAAACGTGTACTATCAGTTCTATCCATTTTTATTATCGATAAATATAATTTT

Coding sequence (CDS)

ATGGCCGACATGGGTTTCCGGAATGTGATAGCATCCATGGATCCATTGGCTACCTCCCGTAAGATACCTCTGCTCAATGCCACTGCCATTGGGGAGTCTCCTGATACCCAAGATGATCATCTCATTATACCGGATCATCTATTCTCTTCCCGTGCTCGTATTTCCTCCCACGATCAGTATTTGGAGATGTACAATAGGTCCATTGATGACCCTTCTGGATTTTGGTCCGATATTGCGGCTTCTGAGTTCTTTTGGAAACAGAAATGGGATCAGAAAGTCTACTCTGAGAATCTTGATGTCCGGAAAGGTGAAATCAAGATAGAGTGGTTCAAAGGTGGAATCACCAACATTTGTTACAACTGTCTTGATAGAAACATTGAAGCTGGACTTGGGGAAAAGGTTGCTCTCTACTGGGAAGGAAACGAGCCTGGTTTTGATAGCTCCTTAACTTACACTCACCTTCTAAACAAAGTTTGTCAGCTAGCAAGCTTCTTGAAAGCTAATGGAGTCAGGAAAGGTGATGCTGTTGTGATCTATATGCCCATGCTAATGGAGCTTCCAATCGCCATGCTTGCCTGTGCTAGAATTGGTGCTATACATTCGGTCGTTTTTGGGGGGTTTTCTTCAGAATCTCTTGCTCAAAGAATCATAGATTGTAAACCAAAAATTGTGATTACTTGCAATGCTGTTAAAAGGGGTTCTAAAGTTATCCATCTTAAAGACATTGTGGATGTTGCTCTCACAGATTCAGCACAAAATGGAGTCTCTAACGAGTCAGCGCTAAAGAGAGAATCCACTAAATGGAAGGAAGGGAGGGATATATGGTGGCAGGATGCTGTCTCAACATATCCAACCACATGTACAGTGGAATGGGTTGATGCAGAGGATCCACTTTTCCTGCTTTATACTAGCGGGAGTACAGGAAAGCCAAAGGGTGTTCTTCATACAACTGGAGGATACATGGTGTATACAGCAACGACATTCAAGTATGCATTTGATTACAAACTGACCGATGTGTACTGGTGTACAGCTGACTGTGGTTGGATCACTGGGCACAGTTATGTCACGTATGGACCTTTACTCAATGGAGCTAGTGTTGTTGTTTTTGAAGGGGCCCCAAATTATCCCGATCCTGGACGTTGTTGGGACATAGTTGATAAGTATAAGGTGACAATATTCTATACCGCTCCAACATTAGTACGGTCCCTCATGCGTGAAGGGAACGAGCATGTTACTCGCTACTCCCGAAAATCCTTACGGGTTCTTGGAAGCGTGGGCGAGCCAATCAATCCCAGTGCATGGAGGTGGTTCTTCGATGTCGTTGGAGAGTCGAGGTGTCCTATATCGGACACATGGTGGCAAACTGAAACTGGTGGTTTCATGATAACTCCATTACCAGGCGCATGGCCTCAGAAGCCAGGCTCTGCTACTTTTCCCTTTTTTGGAGTTCAGCCCATTCTGGTGGATGAAAAAGGTAATGAACTTAAAGGTGAATGCAGTGGATATCTATGCTTGAAAAGCTCATGGCCTTCAGCATTTAGAACTCTTTACAATGATCAAGAAAGATATGAAACAACCTACTTCAAGCCATTCCCTGGCTATTACTTTACTGGCGATGGCTGCACCAGGGATAAGGATGGATACTACTGGCTTACTGGAAGAGTTGATGATGTTATCAATGTCAGTGGGCATCGTATTGGCACAGCAGAAGTGGAATCTGCTTTAGTTTCACATCCCCAATGTGCCGAAGCAGCTGTAGTTGGTATCGAGCACGAGGTGAAAGGACAGGGGATTTATGCATTTGTGACTCTAGTGGATGGCGTTTCTTATAGTGAAGAGCTAAGGAAAAGCCTTATTCTCACGGTTAGAAACCAGATAGGAGCATTTGCAGCACCCGACAAAATCCATTGGGCGCCTGGACTTCCAAAAACAAGGAGCGGTAAAATAATGAGAAGAATTCTAAGGAAAATTGCGTCCATGCAGCTAGATGAACTTGGAGACACTAGCACTCTAGCAGATCCTGATGTGGTTCATCACCTCATCAGCCTTGCTGATCGCTGA

Protein sequence

MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQYLEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDVALTDSAQNGVSNESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASMQLDELGDTSTLADPDVVHHLISLADR
Homology
BLAST of Carg25318 vs. NCBI nr
Match: KAG7022600.1 (Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1435.2 bits (3714), Expect = 0.0e+00
Identity = 686/686 (100.00%), Postives = 686/686 (100.00%), Query Frame = 0

Query: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60
           MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY
Sbjct: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60

Query: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120
           LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN
Sbjct: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120

Query: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180
           CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM
Sbjct: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180

Query: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK 240
           PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK
Sbjct: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK 240

Query: 241 DIVDVALTDSAQNGVSNESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAEDPLFL 300
           DIVDVALTDSAQNGVSNESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAEDPLFL
Sbjct: 241 DIVDVALTDSAQNGVSNESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAEDPLFL 300

Query: 301 LYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYVTYGPL 360
           LYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYVTYGPL
Sbjct: 301 LYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYVTYGPL 360

Query: 361 LNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLR 420
           LNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLR
Sbjct: 361 LNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLR 420

Query: 421 VLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPF 480
           VLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPF
Sbjct: 421 VLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPF 480

Query: 481 FGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDG 540
           FGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDG
Sbjct: 481 FGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDG 540

Query: 541 CTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYA 600
           CTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYA
Sbjct: 541 CTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYA 600

Query: 601 FVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASM 660
           FVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASM
Sbjct: 601 FVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASM 660

Query: 661 QLDELGDTSTLADPDVVHHLISLADR 687
           QLDELGDTSTLADPDVVHHLISLADR
Sbjct: 661 QLDELGDTSTLADPDVVHHLISLADR 686

BLAST of Carg25318 vs. NCBI nr
Match: XP_022928338.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal-like [Cucurbita moschata])

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 674/694 (97.12%), Postives = 677/694 (97.55%), Query Frame = 0

Query: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60
           MADMGFRNVIASMDPLA SRKIPLLNATAIGES DTQDDHLIIPDH FSSRA ISSHDQY
Sbjct: 1   MADMGFRNVIASMDPLANSRKIPLLNATAIGESADTQDDHLIIPDHQFSSRACISSHDQY 60

Query: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120
           LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVR+GEIKIEWFKGGITNICYN
Sbjct: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRRGEIKIEWFKGGITNICYN 120

Query: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180
           CLDRNIEAGLG+KVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM
Sbjct: 121 CLDRNIEAGLGQKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180

Query: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK 240
           PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVI LK
Sbjct: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVILLK 240

Query: 241 DIVDVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300
           DIVDVALTDSAQNGVS        NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV
Sbjct: 241 DIVDVALTDSAQNGVSVATCLTYENESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300

Query: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGH 360
           DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK TDVYWCTADCGWITGH
Sbjct: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVYWCTADCGWITGH 360

Query: 361 SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVT 420
           SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKY VTIFYTAPTLVRSLMREGNEHVT
Sbjct: 361 SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYTVTIFYTAPTLVRSLMREGNEHVT 420

Query: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480
           RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK
Sbjct: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480

Query: 481 PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540
           PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP
Sbjct: 481 PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540

Query: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600
           GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE
Sbjct: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600

Query: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 660
           VKGQGIYAFVTLVDGVSYSEELRKSLIL VRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR
Sbjct: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILAVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 660

Query: 661 ILRKIASMQLDELGDTSTLADPDVVHHLISLADR 687
           ILRKIAS QLDE+GDTSTLADPDVVHHLISLADR
Sbjct: 661 ILRKIASKQLDEVGDTSTLADPDVVHHLISLADR 694

BLAST of Carg25318 vs. NCBI nr
Match: XP_023529989.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1399.8 bits (3622), Expect = 0.0e+00
Identity = 671/694 (96.69%), Postives = 678/694 (97.69%), Query Frame = 0

Query: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60
           MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDH FSSRA ISSHDQY
Sbjct: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHQFSSRACISSHDQY 60

Query: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120
           LEMYNRS+DDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN
Sbjct: 61  LEMYNRSVDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120

Query: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180
           CLDRNIEAGLG KVALYWEGNEPGFD+SLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM
Sbjct: 121 CLDRNIEAGLGAKVALYWEGNEPGFDASLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180

Query: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK 240
           PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPK VITCNAVKRGSKVIHLK
Sbjct: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKTVITCNAVKRGSKVIHLK 240

Query: 241 DIVDVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300
           DIVDVALTDSAQNGVS        NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV
Sbjct: 241 DIVDVALTDSAQNGVSVATCLTYENESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300

Query: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGH 360
           DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK +DVYWCTADCGWITGH
Sbjct: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGH 360

Query: 361 SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVT 420
           SYVTYGPLLNGASVVV+EGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVT
Sbjct: 361 SYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVT 420

Query: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480
           RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK
Sbjct: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480

Query: 481 PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540
           PGSATFPFFGV+P+LVDEKGNE+KGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP
Sbjct: 481 PGSATFPFFGVEPVLVDEKGNEIKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540

Query: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600
           GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE
Sbjct: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600

Query: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 660
           VKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWA GLPKTRSGKIMRR
Sbjct: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWALGLPKTRSGKIMRR 660

Query: 661 ILRKIASMQLDELGDTSTLADPDVVHHLISLADR 687
           ILRKIAS QLDELGDTSTLADPDVVH LISLADR
Sbjct: 661 ILRKIASKQLDELGDTSTLADPDVVHQLISLADR 694

BLAST of Carg25318 vs. NCBI nr
Match: KAG6588847.1 (Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1332.0 bits (3446), Expect = 0.0e+00
Identity = 650/696 (93.39%), Postives = 650/696 (93.39%), Query Frame = 0

Query: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60
           MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY
Sbjct: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60

Query: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120
           LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN
Sbjct: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120

Query: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQ--LASFLKANGVRKGDAVVI 180
           CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQ  LASFLKANGVRKGDAVVI
Sbjct: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQVILASFLKANGVRKGDAVVI 180

Query: 181 YMPMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIH 240
           YMPMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIH
Sbjct: 181 YMPMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIH 240

Query: 241 LKDIVDVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVE 300
           LKDIVDVALTDSAQNGVS        NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVE
Sbjct: 241 LKDIVDVALTDSAQNGVSVATCLTYENESALKRESTKWKEGRDIWWQDAVSTYPTTCTVE 300

Query: 301 WVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWIT 360
           WVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWIT
Sbjct: 301 WVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWIT 360

Query: 361 GHSYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEH 420
           GHSYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEH
Sbjct: 361 GHSYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEH 420

Query: 421 VTRYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWP 480
           VTRYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWP
Sbjct: 421 VTRYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWP 480

Query: 481 QKPGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKP 540
           QKPGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKP
Sbjct: 481 QKPGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKP 540

Query: 541 FPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIE 600
           FPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIE
Sbjct: 541 FPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIE 600

Query: 601 HEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIM 660
           HEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRN                          
Sbjct: 601 HEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRN-------------------------- 660

Query: 661 RRILRKIASMQLDELGDTSTLADPDVVHHLISLADR 687
                     QLDELGDTSTLADPDVVHHLISLADR
Sbjct: 661 ----------QLDELGDTSTLADPDVVHHLISLADR 660

BLAST of Carg25318 vs. NCBI nr
Match: XP_008449961.1 (PREDICTED: acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X1 [Cucumis melo])

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 620/690 (89.86%), Postives = 644/690 (93.33%), Query Frame = 0

Query: 4   MGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQYLEM 63
           MG    +A++D  A +RKIP LNA  +GESP T+DD LI+P   FSS A ISS+ QYLEM
Sbjct: 122 MGGFTDVAALDSSA-ARKIPRLNAAVLGESPATEDDQLILPSDQFSSNANISSYHQYLEM 181

Query: 64  YNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYNCLD 123
           Y RSI+DP+GFWSDIA+SEFFWKQKWDQ+VYSENLDVRKGEIKIEWFKGGITNICYNCLD
Sbjct: 182 YKRSIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFKGGITNICYNCLD 241

Query: 124 RNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYMPML 183
           RNIEAGLGEK+ALYWEGNEPGFD +LTY  LL+KVCQLA+FLK NGVRKGDAVVIYMPML
Sbjct: 242 RNIEAGLGEKIALYWEGNEPGFDGTLTYIELLHKVCQLANFLKDNGVRKGDAVVIYMPML 301

Query: 184 MELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIV 243
           MELPIAMLACARIGA+HSVVF GFSSESLAQRIIDCKPKIVITCNAVKRGSK IHLKDIV
Sbjct: 302 MELPIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKAIHLKDIV 361

Query: 244 DVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAE 303
           D AL DSAQNGVS        NESAL RESTKWKEGRDIWWQD V TYPTTCTVEWVDAE
Sbjct: 362 DAALIDSAQNGVSVATCLCYENESALNRESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAE 421

Query: 304 DPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYV 363
            PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK +DVYWCTADCGWITGHSYV
Sbjct: 422 FPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYV 481

Query: 364 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 423
           TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS
Sbjct: 482 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 541

Query: 424 RKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 483
           RKSLRVLGSVGEPINPSAWRWFF+VVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS
Sbjct: 542 RKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 601

Query: 484 ATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYY 543
           ATFPFFGVQP+LVDEKGNE++GECSGYLCLKSSWPSAFRTLYNDQERYE TYFKPFPGYY
Sbjct: 602 ATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERYEVTYFKPFPGYY 661

Query: 544 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKG 603
           FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG+EHEVKG
Sbjct: 662 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKG 721

Query: 604 QGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 663
           QGIYAFVTLVDGVSYSEELRKSLILTVR QIGAFAAPDKIHWAPGLPKTRSGKIMRRILR
Sbjct: 722 QGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 781

Query: 664 KIASMQLDELGDTSTLADPDVVHHLISLAD 686
           KIAS QLDELGDTSTLADP VV+ LI LAD
Sbjct: 782 KIASKQLDELGDTSTLADPGVVNQLIELAD 810

BLAST of Carg25318 vs. ExPASy Swiss-Prot
Match: B9DGD6 (Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal OS=Arabidopsis thaliana OX=3702 GN=ACS PE=1 SV=1)

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 542/693 (78.21%), Postives = 614/693 (88.60%), Query Frame = 0

Query: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60
           M+    R+V +     + + KI  LNA  +GES  ++++ L+ P   FS +A +SS  QY
Sbjct: 51  MSSNSLRHVESMSQLPSGAGKISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQY 110

Query: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120
           +EM+ RS+DDP+ FWSDI ASEF+WKQKW  +V+SENLDVRKG I IEWFKGGITNICYN
Sbjct: 111 MEMHKRSMDDPAAFWSDI-ASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYN 170

Query: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180
           CLD+N+EAGLG+K A++WEGNE G D+SLTY+ LL +VCQLA++LK NGV+KGDAVVIY+
Sbjct: 171 CLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYL 230

Query: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK 240
           PMLMELPIAMLACARIGA+HSVVF GFS++SLAQRI+DCKP +++TCNAVKRG K I+LK
Sbjct: 231 PMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLK 290

Query: 241 DIVDVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300
            IVD AL  S+++GVS        N  A  RE+TKW+ GRD+WWQD +S YPT+C VEWV
Sbjct: 291 AIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWV 350

Query: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGH 360
           DAEDPLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK TDVYWCTADCGWITGH
Sbjct: 351 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGH 410

Query: 361 SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVT 420
           SYVTYGP+LNGA+VVVFEGAPNYPDPGRCWDIVDKYKV+IFYTAPTLVRSLMR+ ++ VT
Sbjct: 411 SYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVT 470

Query: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480
           R+SRKSLRVLGSVGEPINPSAWRWFF+VVG+SRCPISDTWWQTETGGFMITPLPGAWPQK
Sbjct: 471 RHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQK 530

Query: 481 PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540
           PGSATFPFFGVQP++VDEKGNE++GECSGYLC+K SWP AFRTL+ D ERYETTYFKPF 
Sbjct: 531 PGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFA 590

Query: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600
           GYYF+GDGC+RDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV HPQCAEAAVVGIEHE
Sbjct: 591 GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHE 650

Query: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 660
           VKGQGIYAFVTL++GV YSEELRKSL+L VRNQIGAFAAPD+IHWAPGLPKTRSGKIMRR
Sbjct: 651 VKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRR 710

Query: 661 ILRKIASMQLDELGDTSTLADPDVVHHLISLAD 686
           ILRKIAS QL+ELGDTSTLADP VV  LI+LAD
Sbjct: 711 ILRKIASRQLEELGDTSTLADPSVVDQLIALAD 742

BLAST of Carg25318 vs. ExPASy Swiss-Prot
Match: Q9NR19 (Acetyl-coenzyme A synthetase, cytoplasmic OS=Homo sapiens OX=9606 GN=ACSS2 PE=1 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 5.9e-230
Identity = 384/661 (58.09%), Postives = 464/661 (70.20%), Query Frame = 0

Query: 49  SSRARISSHDQYLEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIE 108
           S  A + S  +Y E++ RS+++P  FW DI A EF+WK          N DV KG+I IE
Sbjct: 36  SRSAHVPSLQRYRELHRRSVEEPREFWGDI-AKEFYWKTPCPGPFLRYNFDVTKGKIFIE 95

Query: 109 WFKGGITNICYNCLDRNI-EAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKA 168
           W KG  TNICYN LDRN+ E  LG+KVA YWEGNEPG  + +TY  LL +VCQ ++ L+ 
Sbjct: 96  WMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTQITYHQLLVQVCQFSNVLRK 155

Query: 169 NGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITC 228
            G++KGD V IYMPM+ EL +AMLACARIGA+HS+VF GFSSESL +RI+D    ++IT 
Sbjct: 156 QGIQKGDRVAIYMPMIPELVVAMLACARIGALHSIVFAGFSSESLCERILDSSCSLLITT 215

Query: 229 NAVKRGSKVIHLKDIVDVALTDSAQNG----------------------VSNESALKRE- 288
           +A  RG K+++LK++ D AL    + G                       S    +KR  
Sbjct: 216 DAFYRGEKLVNLKELADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPPIKRSC 275

Query: 289 ---STKWKEGRDIWWQDAVSTYPTTCTVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYM 348
                 W +G D+WW + +      C  EW DAEDPLF+LYTSGSTGKPKGV+HT GGYM
Sbjct: 276 PDVQISWNQGIDLWWHELMQEAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTVGGYM 335

Query: 349 VYTATTFKYAFDYKLTDVYWCTADCGWITGHSYVTYGPLLNGASVVVFEGAPNYPDPGRC 408
           +Y ATTFKY FD+   DV+WCTAD GWITGHSYVTYGPL NGA+ V+FEG P YPD  R 
Sbjct: 336 LYVATTFKYVFDFHAEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDVNRL 395

Query: 409 WDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFDVV 468
           W IVDKYKVT FYTAPT +R LM+ G+E VT++SR SL+VLG+VGEPINP AW W+  VV
Sbjct: 396 WSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLWYHRVV 455

Query: 469 GESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPILVDEKGNELKGECSG 528
           G  RCPI DT+WQTETGG M+TPLPGA P KPGSATFPFFGV P +++E G EL+GE  G
Sbjct: 456 GAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELEGEAEG 515

Query: 529 YLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINV 588
           YL  K  WP   RT+Y + ER+ETTYFK FPGYY TGDGC RD+DGYYW+TGR+DD++NV
Sbjct: 516 YLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNV 575

Query: 589 SGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILT 648
           SGH + TAEVESALV H   AEAAVVG  H VKG+ +Y FVTL DG ++S +L + L   
Sbjct: 576 SGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTEELKKQ 635

Query: 649 VRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASMQLDELGDTSTLADPDVVHHLI 683
           +R +IG  A PD I  APGLPKTRSGKIMRR+LRKIA    D LGD ST+ADP V+ HL 
Sbjct: 636 IREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHD-LGDMSTVADPSVISHLF 694

BLAST of Carg25318 vs. ExPASy Swiss-Prot
Match: Q9QXG4 (Acetyl-coenzyme A synthetase, cytoplasmic OS=Mus musculus OX=10090 GN=Acss2 PE=1 SV=2)

HSP 1 Score: 791.6 bits (2043), Expect = 7.2e-228
Identity = 378/658 (57.45%), Postives = 465/658 (70.67%), Query Frame = 0

Query: 52  ARISSHDQYLEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFK 111
           A + S  +Y E++ RS+++P  FW +I A EF+WK          N DV KG+I  EW K
Sbjct: 39  AHVPSLQRYRELHRRSVEEPREFWGNI-AKEFYWKTACPGPFLQYNFDVTKGKIFTEWMK 98

Query: 112 GGITNICYNCLDRNI-EAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGV 171
           G  TNICYN LDRN+ E  LG+KVA YWEGNEPG  + +TY  LL +VCQ ++ L+  G+
Sbjct: 99  GATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLRKQGI 158

Query: 172 RKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAV 231
           +KGD V IYMPM++EL +AMLACAR+GA+HS+VF GFS+ESL +RI+D    ++IT +A 
Sbjct: 159 QKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAF 218

Query: 232 KRGSKVIHLKDIVDVALTDSAQNGV-----------------SNESALKRESTK------ 291
            RG K+++LK++ D +L    + G                   N+S  +    K      
Sbjct: 219 YRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDV 278

Query: 292 ---WKEGRDIWWQDAVSTYPTTCTVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYT 351
              W EG D+WW + +      C  EW DAEDPLF+LYTSGSTGKPKGV+HT GGYM+Y 
Sbjct: 279 QICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYV 338

Query: 352 ATTFKYAFDYKLTDVYWCTADCGWITGHSYVTYGPLLNGASVVVFEGAPNYPDPGRCWDI 411
           ATTFKY FD+   DV+WCTAD GWITGHSYVTYGPL NGA+ V+FEG P YPD GR W I
Sbjct: 339 ATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSI 398

Query: 412 VDKYKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFDVVGES 471
           VDKYKVT FYTAPT +R LM+ G++ VT++SR SL+VLG+VGEPINP AW W+  VVG  
Sbjct: 399 VDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQ 458

Query: 472 RCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPILVDEKGNELKGECSGYLC 531
           RCPI DT+WQTETGG M+TPLPGA P KPGSA+FPFFGV P +++E G EL+GE  GYL 
Sbjct: 459 RCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLV 518

Query: 532 LKSSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGH 591
            K  WP   RT+Y +  R+ETTYFK FPGYY TGDGC RD+DGYYW+TGR+DD++NVSGH
Sbjct: 519 FKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGH 578

Query: 592 RIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRN 651
            + TAEVESALV H   AEAAVVG  H VKG+ +Y FVTL DG ++S  L + L   +R 
Sbjct: 579 LLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIRE 638

Query: 652 QIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASMQLDELGDTSTLADPDVVHHLIS 683
           +IG  A PD I  APGLPKTRSGKIMRR+LRKIA    D LGDTST+ADP V++HL S
Sbjct: 639 KIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHD-LGDTSTVADPSVINHLFS 694

BLAST of Carg25318 vs. ExPASy Swiss-Prot
Match: Q0AAW0 (Acetyl-coenzyme A synthetase OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=acsA PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.5e-225
Identity = 376/641 (58.66%), Postives = 470/641 (73.32%), Query Frame = 0

Query: 43  IPDHLFSSRARISSHDQYLEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRK 102
           +PD++   +A I+  D Y EMY RS+DDP GFW + A     W  KWD KVY  + D++ 
Sbjct: 9   VPDYI-REKAHITK-DTYEEMYRRSLDDPEGFWGEQAEKFLDWFSKWD-KVY--HSDLKN 68

Query: 103 GEIKIEWFKGGITNICYNCLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLA 162
           GEI+  +F+GG  N+ +NCLDR++E   G++ A+ WEG++P     +TY  L  +VC+LA
Sbjct: 69  GEIR--FFEGGKLNVAHNCLDRHLEQ-RGDQTAIIWEGDDPNNSEHITYKDLHERVCRLA 128

Query: 163 SFLKANGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPK 222
           + +KA GV+KGD V IY+PM+ E  +AMLACARIGAIHS+VFGGFS ++L  RI +   +
Sbjct: 129 NAMKARGVKKGDRVCIYLPMIPEAAVAMLACARIGAIHSIVFGGFSPDALKDRIQNADCE 188

Query: 223 IVITCNAVKRGSKVIHLKDIVDVALTDSAQNGVSNESALKRE--STKWKEGRDIWWQDAV 282
            VIT +   RG + + LK   D AL       V N   +KR      WKEGRDIW+ +AV
Sbjct: 189 TVITADEGVRGGRNVALKSNADKALESCPD--VKNVFVVKRTGGDIDWKEGRDIWYHEAV 248

Query: 283 STYPTTCTVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVY 342
           +     C  E +DAEDPLF+LYTSGSTGKPKGV H + GY++  A T KY FDY+  +VY
Sbjct: 249 ADVSADCPAEELDAEDPLFILYTSGSTGKPKGVQHCSAGYLLGAAMTHKYVFDYQEGEVY 308

Query: 343 WCTADCGWITGHSYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLV 402
           WCTAD GW+TGHSY+ YGPL NGA+ ++FEG P YP   RCW++VDK+ V+IFYTAPT +
Sbjct: 309 WCTADVGWVTGHSYIVYGPLANGATTLMFEGVPTYPSAARCWEVVDKHNVSIFYTAPTAI 368

Query: 403 RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGF 462
           R+LM +GNEHVT+ SRKSLR+LG+VGEPINP AW W+++VVG+ RCPI DTWWQTETG  
Sbjct: 369 RALMGQGNEHVTKTSRKSLRILGTVGEPINPEAWEWYYNVVGDGRCPIVDTWWQTETGSI 428

Query: 463 MITPLPGAWPQKPGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQ 522
           +I PLPGA   KPGSAT PFFGV+P LVD +G EL+G  SG L +K +WPS  RT+Y D 
Sbjct: 429 LIAPLPGATDLKPGSATLPFFGVEPALVDPEGKELEGAASGNLVIKRAWPSMMRTVYGDH 488

Query: 523 ERYETTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 582
           +R+  TY   +PG YFTGDG  RD+DGYYW+TGRVDDVINVSGHR+GTAEVESALV H  
Sbjct: 489 KRFMETYLAAYPGMYFTGDGARRDEDGYYWITGRVDDVINVSGHRMGTAEVESALVLHDA 548

Query: 583 CAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPG 642
            AEAAVVG  H++KGQGIYA+VTL+ GV  S+EL+K L+  V N+IG  A PD I WAPG
Sbjct: 549 VAEAAVVGYPHDIKGQGIYAYVTLMAGVEPSDELKKELVKLVSNEIGPIAKPDVIQWAPG 608

Query: 643 LPKTRSGKIMRRILRKIASMQLDELGDTSTLADPDVVHHLI 682
           LPKTRSGKIMRRILRK+A+ +LD LGDTSTLADP VV +LI
Sbjct: 609 LPKTRSGKIMRRILRKVAANELDSLGDTSTLADPTVVDNLI 639

BLAST of Carg25318 vs. ExPASy Swiss-Prot
Match: A0L576 (Acetyl-coenzyme A synthetase OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1) OX=156889 GN=acsA PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 9.8e-225
Identity = 366/626 (58.47%), Postives = 459/626 (73.32%), Query Frame = 0

Query: 56  SHDQYLEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGIT 115
           + +QY EMY RSIDDP+ FW++ A +   W +KWD  +   + D+RKG IK  WF+G   
Sbjct: 20  NEEQYQEMYKRSIDDPAAFWAEQAEARIDWFKKWDNVL---DFDLRKGHIK--WFEGAKL 79

Query: 116 NICYNCLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDA 175
           N+ YNC+DR++E   G+KVA+ WEG+ P  D  +TY  L   V + A+ LKA GV+KGD 
Sbjct: 80  NVSYNCIDRHLEK-RGDKVAILWEGDNPAADRKITYRELSALVNKFANALKARGVKKGDR 139

Query: 176 VVIYMPMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSK 235
           V IYMPM+ E  +AMLAC RIGA+HS+VFGGFS ++L  RIID + ++VIT +   RGSK
Sbjct: 140 VCIYMPMIPEAAVAMLACTRIGAVHSIVFGGFSPDALRDRIIDAECRVVITADGGMRGSK 199

Query: 236 VIHLKDIVDVALTDSAQNGVSNESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAE 295
            I L+  V+ AL               +   +W EGRD+W+ +A++     C  E +DAE
Sbjct: 200 KIPLRKNVETALAQCPNVHTCFVVQDTQLEIEWTEGRDVWYHEAIAAASADCPPEEMDAE 259

Query: 296 DPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYV 355
           DPLF+LYTSGSTGKPKGVLHTTGGY++Y + T +Y FDY   D+YWCTAD GW+TGH+Y+
Sbjct: 260 DPLFVLYTSGSTGKPKGVLHTTGGYLLYASITHQYIFDYHEDDIYWCTADVGWVTGHTYI 319

Query: 356 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 415
            YGPL NGA+ ++FEG P YPD  R W +VDK++V+IFYTAPT +RS+M +G+  V   S
Sbjct: 320 VYGPLANGATTLMFEGVPTYPDASRFWQVVDKHQVSIFYTAPTAIRSIMSQGDALVKATS 379

Query: 416 RKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 475
           R+SLR+LGSVGEPINP AW W++ VVGE RCPI DTWWQTETGG +ITPLPGA P KPGS
Sbjct: 380 RQSLRILGSVGEPINPEAWEWYYHVVGEGRCPIVDTWWQTETGGILITPLPGATPLKPGS 439

Query: 476 ATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYY 535
           AT PFFGV P ++  +G E++G C G L L   WP   RT+Y D +R+  TYF  +PGYY
Sbjct: 440 ATRPFFGVNPKVLSNEGKEMEGPCEGILVLDHPWPGMMRTVYGDHDRFINTYFANYPGYY 499

Query: 536 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKG 595
           F GDGC RD+DGYYW+TGRVDDVINVSGHR+GTAEVESALV H Q AEAAVVG+ HE+KG
Sbjct: 500 FCGDGCRRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVLHDQVAEAAVVGMPHEIKG 559

Query: 596 QGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 655
           QGIYA+VTL+ GV  S+ L+  L+  VR +IG  A+ D I +APGLPKTRSGKIMRRILR
Sbjct: 560 QGIYAYVTLMSGVEPSDALKTELVKLVRKEIGPIASLDVIQFAPGLPKTRSGKIMRRILR 619

Query: 656 KIASMQLDELGDTSTLADPDVVHHLI 682
           KIA+ ++D LGDTSTLADP VV +LI
Sbjct: 620 KIAAHEVDTLGDTSTLADPSVVQNLI 639

BLAST of Carg25318 vs. ExPASy TrEMBL
Match: A0A6J1ENQ8 (Acetyl-coenzyme A synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435195 PE=3 SV=1)

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 674/694 (97.12%), Postives = 677/694 (97.55%), Query Frame = 0

Query: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60
           MADMGFRNVIASMDPLA SRKIPLLNATAIGES DTQDDHLIIPDH FSSRA ISSHDQY
Sbjct: 1   MADMGFRNVIASMDPLANSRKIPLLNATAIGESADTQDDHLIIPDHQFSSRACISSHDQY 60

Query: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120
           LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVR+GEIKIEWFKGGITNICYN
Sbjct: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRRGEIKIEWFKGGITNICYN 120

Query: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180
           CLDRNIEAGLG+KVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM
Sbjct: 121 CLDRNIEAGLGQKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180

Query: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK 240
           PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVI LK
Sbjct: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVILLK 240

Query: 241 DIVDVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300
           DIVDVALTDSAQNGVS        NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV
Sbjct: 241 DIVDVALTDSAQNGVSVATCLTYENESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300

Query: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGH 360
           DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK TDVYWCTADCGWITGH
Sbjct: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVYWCTADCGWITGH 360

Query: 361 SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVT 420
           SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKY VTIFYTAPTLVRSLMREGNEHVT
Sbjct: 361 SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYTVTIFYTAPTLVRSLMREGNEHVT 420

Query: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480
           RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK
Sbjct: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480

Query: 481 PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540
           PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP
Sbjct: 481 PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540

Query: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600
           GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE
Sbjct: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600

Query: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 660
           VKGQGIYAFVTLVDGVSYSEELRKSLIL VRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR
Sbjct: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILAVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 660

Query: 661 ILRKIASMQLDELGDTSTLADPDVVHHLISLADR 687
           ILRKIAS QLDE+GDTSTLADPDVVHHLISLADR
Sbjct: 661 ILRKIASKQLDEVGDTSTLADPDVVHHLISLADR 694

BLAST of Carg25318 vs. ExPASy TrEMBL
Match: A0A1S3BML7 (Acetate--CoA ligase OS=Cucumis melo OX=3656 GN=LOC103491691 PE=3 SV=1)

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 620/690 (89.86%), Postives = 644/690 (93.33%), Query Frame = 0

Query: 4   MGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQYLEM 63
           MG    +A++D  A +RKIP LNA  +GESP T+DD LI+P   FSS A ISS+ QYLEM
Sbjct: 22  MGGFTDVAALDSSA-ARKIPRLNAAVLGESPATEDDQLILPSDQFSSNANISSYHQYLEM 81

Query: 64  YNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYNCLD 123
           Y RSI+DP+GFWSDIA+SEFFWKQKWDQ+VYSENLDVRKGEIKIEWFKGGITNICYNCLD
Sbjct: 82  YKRSIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFKGGITNICYNCLD 141

Query: 124 RNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYMPML 183
           RNIEAGLGEK+ALYWEGNEPGFD +LTY  LL+KVCQLA+FLK NGVRKGDAVVIYMPML
Sbjct: 142 RNIEAGLGEKIALYWEGNEPGFDGTLTYIELLHKVCQLANFLKDNGVRKGDAVVIYMPML 201

Query: 184 MELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIV 243
           MELPIAMLACARIGA+HSVVF GFSSESLAQRIIDCKPKIVITCNAVKRGSK IHLKDIV
Sbjct: 202 MELPIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKAIHLKDIV 261

Query: 244 DVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAE 303
           D AL DSAQNGVS        NESAL RESTKWKEGRDIWWQD V TYPTTCTVEWVDAE
Sbjct: 262 DAALIDSAQNGVSVATCLCYENESALNRESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAE 321

Query: 304 DPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYV 363
            PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK +DVYWCTADCGWITGHSYV
Sbjct: 322 FPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYV 381

Query: 364 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 423
           TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS
Sbjct: 382 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 441

Query: 424 RKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 483
           RKSLRVLGSVGEPINPSAWRWFF+VVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS
Sbjct: 442 RKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 501

Query: 484 ATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYY 543
           ATFPFFGVQP+LVDEKGNE++GECSGYLCLKSSWPSAFRTLYNDQERYE TYFKPFPGYY
Sbjct: 502 ATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERYEVTYFKPFPGYY 561

Query: 544 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKG 603
           FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG+EHEVKG
Sbjct: 562 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKG 621

Query: 604 QGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 663
           QGIYAFVTLVDGVSYSEELRKSLILTVR QIGAFAAPDKIHWAPGLPKTRSGKIMRRILR
Sbjct: 622 QGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 681

Query: 664 KIASMQLDELGDTSTLADPDVVHHLISLAD 686
           KIAS QLDELGDTSTLADP VV+ LI LAD
Sbjct: 682 KIASKQLDELGDTSTLADPGVVNQLIELAD 710

BLAST of Carg25318 vs. ExPASy TrEMBL
Match: A0A1S3BP61 (Acetate--CoA ligase OS=Cucumis melo OX=3656 GN=LOC103491691 PE=3 SV=1)

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 620/690 (89.86%), Postives = 644/690 (93.33%), Query Frame = 0

Query: 4   MGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQYLEM 63
           MG    +A++D  A +RKIP LNA  +GESP T+DD LI+P   FSS A ISS+ QYLEM
Sbjct: 122 MGGFTDVAALDSSA-ARKIPRLNAAVLGESPATEDDQLILPSDQFSSNANISSYHQYLEM 181

Query: 64  YNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYNCLD 123
           Y RSI+DP+GFWSDIA+SEFFWKQKWDQ+VYSENLDVRKGEIKIEWFKGGITNICYNCLD
Sbjct: 182 YKRSIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFKGGITNICYNCLD 241

Query: 124 RNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYMPML 183
           RNIEAGLGEK+ALYWEGNEPGFD +LTY  LL+KVCQLA+FLK NGVRKGDAVVIYMPML
Sbjct: 242 RNIEAGLGEKIALYWEGNEPGFDGTLTYIELLHKVCQLANFLKDNGVRKGDAVVIYMPML 301

Query: 184 MELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIV 243
           MELPIAMLACARIGA+HSVVF GFSSESLAQRIIDCKPKIVITCNAVKRGSK IHLKDIV
Sbjct: 302 MELPIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKAIHLKDIV 361

Query: 244 DVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAE 303
           D AL DSAQNGVS        NESAL RESTKWKEGRDIWWQD V TYPTTCTVEWVDAE
Sbjct: 362 DAALIDSAQNGVSVATCLCYENESALNRESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAE 421

Query: 304 DPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYV 363
            PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK +DVYWCTADCGWITGHSYV
Sbjct: 422 FPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYV 481

Query: 364 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 423
           TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS
Sbjct: 482 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 541

Query: 424 RKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 483
           RKSLRVLGSVGEPINPSAWRWFF+VVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS
Sbjct: 542 RKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 601

Query: 484 ATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYY 543
           ATFPFFGVQP+LVDEKGNE++GECSGYLCLKSSWPSAFRTLYNDQERYE TYFKPFPGYY
Sbjct: 602 ATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERYEVTYFKPFPGYY 661

Query: 544 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKG 603
           FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG+EHEVKG
Sbjct: 662 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKG 721

Query: 604 QGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 663
           QGIYAFVTLVDGVSYSEELRKSLILTVR QIGAFAAPDKIHWAPGLPKTRSGKIMRRILR
Sbjct: 722 QGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 781

Query: 664 KIASMQLDELGDTSTLADPDVVHHLISLAD 686
           KIAS QLDELGDTSTLADP VV+ LI LAD
Sbjct: 782 KIASKQLDELGDTSTLADPGVVNQLIELAD 810

BLAST of Carg25318 vs. ExPASy TrEMBL
Match: A0A5A7U007 (Acetate--CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold61G001500 PE=3 SV=1)

HSP 1 Score: 1295.0 bits (3350), Expect = 0.0e+00
Identity = 619/690 (89.71%), Postives = 643/690 (93.19%), Query Frame = 0

Query: 4   MGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQYLEM 63
           MG    +A++D  A +RKIP LNA  +GESP T+DD LI+P   FSS A ISS+ QYLEM
Sbjct: 1   MGGFTDVAALDSSA-ARKIPRLNAAVLGESPATEDDQLILPSDQFSSNANISSYHQYLEM 60

Query: 64  YNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYNCLD 123
           Y RSI+DP+GFWSDIA+SEFFWKQKWDQ+VYSENLDVRKGEIKIEWFKGGITNICYNCLD
Sbjct: 61  YKRSIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFKGGITNICYNCLD 120

Query: 124 RNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYMPML 183
           RNIEAGLGEK+ALYWEGNEPGFD +LTY  LL+KVCQLA+FLK NGVRKGDAVVIYMPML
Sbjct: 121 RNIEAGLGEKIALYWEGNEPGFDGTLTYIELLHKVCQLANFLKDNGVRKGDAVVIYMPML 180

Query: 184 MELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIV 243
           MELPIAMLACARIGA+HSVVF GFSSESLAQRIIDCKPKIVITCNAVKRGSK IHLKDIV
Sbjct: 181 MELPIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKAIHLKDIV 240

Query: 244 DVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAE 303
           D AL DSAQNGVS        NESAL RESTKWKEGRDIWWQD V TYPTTCTVEWVDAE
Sbjct: 241 DAALIDSAQNGVSVATCLCYENESALNRESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAE 300

Query: 304 DPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYV 363
            PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK +DVYWCTADCGWITGHSYV
Sbjct: 301 FPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYV 360

Query: 364 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 423
           TYGPLLNG SVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS
Sbjct: 361 TYGPLLNGTSVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 420

Query: 424 RKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 483
           RKSLRVLGSVGEPINPSAWRWFF+VVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS
Sbjct: 421 RKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 480

Query: 484 ATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYY 543
           ATFPFFGVQP+LVDEKGNE++GECSGYLCLKSSWPSAFRTLYNDQERYE TYFKPFPGYY
Sbjct: 481 ATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERYEVTYFKPFPGYY 540

Query: 544 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKG 603
           FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG+EHEVKG
Sbjct: 541 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKG 600

Query: 604 QGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 663
           QGIYAFVTLVDGVSYSEELRKSLILTVR QIGAFAAPDKIHWAPGLPKTRSGKIMRRILR
Sbjct: 601 QGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 660

Query: 664 KIASMQLDELGDTSTLADPDVVHHLISLAD 686
           KIAS QLDELGDTSTLADP VV+ LI LAD
Sbjct: 661 KIASKQLDELGDTSTLADPGVVNQLIELAD 689

BLAST of Carg25318 vs. ExPASy TrEMBL
Match: A0A0A0L7C4 (Acetate--CoA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G253500 PE=3 SV=1)

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 616/690 (89.28%), Postives = 641/690 (92.90%), Query Frame = 0

Query: 4   MGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQYLEM 63
           MG    +A+++  A +RKIP LNA  +GESP T+DD LI P  LFSS A ISS+ QYLEM
Sbjct: 115 MGGLTDVAALESSA-ARKIPRLNAAVLGESPATEDDQLIFPSDLFSSNAHISSYHQYLEM 174

Query: 64  YNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYNCLD 123
           Y RSI+DP+GFWSDIA+SEFFWKQKWDQ+VYSENLDVRKGEIKIEWFKGGITNICYNCLD
Sbjct: 175 YKRSIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFKGGITNICYNCLD 234

Query: 124 RNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYMPML 183
           RNIEAGLGEK+ALYWEGNEPGFD +LTY  LL+KVCQLA+FLK  GVRKGDAVVIYMPML
Sbjct: 235 RNIEAGLGEKIALYWEGNEPGFDGTLTYIELLHKVCQLANFLKDKGVRKGDAVVIYMPML 294

Query: 184 MELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIV 243
           MELPIAMLACARIGA+HSVVF GFSSESLAQRIIDCKPKIVITCNAVKRGSK IHLKDIV
Sbjct: 295 MELPIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKAIHLKDIV 354

Query: 244 DVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAE 303
           D AL +SAQNGVS        NESAL RESTKWKEGRDIWWQD V TYPTTC VEWVDAE
Sbjct: 355 DAALIESAQNGVSVATCLSYENESALNRESTKWKEGRDIWWQDVVPTYPTTCPVEWVDAE 414

Query: 304 DPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGHSYV 363
            PLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYK +DVYWCTADCGWITGHSYV
Sbjct: 415 FPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYV 474

Query: 364 TYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 423
           TYGPLLNGASVVVFEGAPNYPD GRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS
Sbjct: 475 TYGPLLNGASVVVFEGAPNYPDHGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYS 534

Query: 424 RKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 483
           RKSLRVLGSVGEPINPSAWRWFF+VVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS
Sbjct: 535 RKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGS 594

Query: 484 ATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYY 543
           ATFPFFGVQP+LVDEKGNE++GECSGYLCLKSSWPSAFRTLYNDQERYE TYFKPFPGYY
Sbjct: 595 ATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERYEVTYFKPFPGYY 654

Query: 544 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKG 603
           FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVG+EHEVKG
Sbjct: 655 FTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKG 714

Query: 604 QGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 663
           QGIYAFVTLVDGVSYSEELRKSLILTVR QIGAFAAPDKIHWAPGLPKTRSGKIMRRILR
Sbjct: 715 QGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 774

Query: 664 KIASMQLDELGDTSTLADPDVVHHLISLAD 686
           KIAS QLDELGDTSTLADP VV+ LI LAD
Sbjct: 775 KIASKQLDELGDTSTLADPGVVNQLIELAD 803

BLAST of Carg25318 vs. TAIR 10
Match: AT5G36880.1 (acetyl-CoA synthetase )

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 542/693 (78.21%), Postives = 614/693 (88.60%), Query Frame = 0

Query: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60
           M+    R+V +     + + KI  LNA  +GES  ++++ L+ P   FS +A +SS  QY
Sbjct: 1   MSSNSLRHVESMSQLPSGAGKISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQY 60

Query: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120
           +EM+ RS+DDP+ FWSDI ASEF+WKQKW  +V+SENLDVRKG I IEWFKGGITNICYN
Sbjct: 61  MEMHKRSMDDPAAFWSDI-ASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYN 120

Query: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180
           CLD+N+EAGLG+K A++WEGNE G D+SLTY+ LL +VCQLA++LK NGV+KGDAVVIY+
Sbjct: 121 CLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYL 180

Query: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK 240
           PMLMELPIAMLACARIGA+HSVVF GFS++SLAQRI+DCKP +++TCNAVKRG K I+LK
Sbjct: 181 PMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLK 240

Query: 241 DIVDVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300
            IVD AL  S+++GVS        N  A  RE+TKW+ GRD+WWQD +S YPT+C VEWV
Sbjct: 241 AIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWV 300

Query: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGH 360
           DAEDPLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK TDVYWCTADCGWITGH
Sbjct: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGH 360

Query: 361 SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVT 420
           SYVTYGP+LNGA+VVVFEGAPNYPDPGRCWDIVDKYKV+IFYTAPTLVRSLMR+ ++ VT
Sbjct: 361 SYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVT 420

Query: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480
           R+SRKSLRVLGSVGEPINPSAWRWFF+VVG+SRCPISDTWWQTETGGFMITPLPGAWPQK
Sbjct: 421 RHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQK 480

Query: 481 PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540
           PGSATFPFFGVQP++VDEKGNE++GECSGYLC+K SWP AFRTL+ D ERYETTYFKPF 
Sbjct: 481 PGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFA 540

Query: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600
           GYYF+GDGC+RDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV HPQCAEAAVVGIEHE
Sbjct: 541 GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHE 600

Query: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 660
           VKGQGIYAFVTL++GV YSEELRKSL+L VRNQIGAFAAPD+IHWAPGLPKTRSGKIMRR
Sbjct: 601 VKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRR 660

Query: 661 ILRKIASMQLDELGDTSTLADPDVVHHLISLAD 686
           ILRKIAS QL+ELGDTSTLADP VV  LI+LAD
Sbjct: 661 ILRKIASRQLEELGDTSTLADPSVVDQLIALAD 692

BLAST of Carg25318 vs. TAIR 10
Match: AT5G36880.2 (acetyl-CoA synthetase )

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 542/693 (78.21%), Postives = 614/693 (88.60%), Query Frame = 0

Query: 1   MADMGFRNVIASMDPLATSRKIPLLNATAIGESPDTQDDHLIIPDHLFSSRARISSHDQY 60
           M+    R+V +     + + KI  LNA  +GES  ++++ L+ P   FS +A +SS  QY
Sbjct: 51  MSSNSLRHVESMSQLPSGAGKISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQY 110

Query: 61  LEMYNRSIDDPSGFWSDIAASEFFWKQKWDQKVYSENLDVRKGEIKIEWFKGGITNICYN 120
           +EM+ RS+DDP+ FWSDI ASEF+WKQKW  +V+SENLDVRKG I IEWFKGGITNICYN
Sbjct: 111 MEMHKRSMDDPAAFWSDI-ASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYN 170

Query: 121 CLDRNIEAGLGEKVALYWEGNEPGFDSSLTYTHLLNKVCQLASFLKANGVRKGDAVVIYM 180
           CLD+N+EAGLG+K A++WEGNE G D+SLTY+ LL +VCQLA++LK NGV+KGDAVVIY+
Sbjct: 171 CLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYL 230

Query: 181 PMLMELPIAMLACARIGAIHSVVFGGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLK 240
           PMLMELPIAMLACARIGA+HSVVF GFS++SLAQRI+DCKP +++TCNAVKRG K I+LK
Sbjct: 231 PMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLK 290

Query: 241 DIVDVALTDSAQNGVS--------NESALKRESTKWKEGRDIWWQDAVSTYPTTCTVEWV 300
            IVD AL  S+++GVS        N  A  RE+TKW+ GRD+WWQD +S YPT+C VEWV
Sbjct: 291 AIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWV 350

Query: 301 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKLTDVYWCTADCGWITGH 360
           DAEDPLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK TDVYWCTADCGWITGH
Sbjct: 351 DAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGH 410

Query: 361 SYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVT 420
           SYVTYGP+LNGA+VVVFEGAPNYPDPGRCWDIVDKYKV+IFYTAPTLVRSLMR+ ++ VT
Sbjct: 411 SYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVT 470

Query: 421 RYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCPISDTWWQTETGGFMITPLPGAWPQK 480
           R+SRKSLRVLGSVGEPINPSAWRWFF+VVG+SRCPISDTWWQTETGGFMITPLPGAWPQK
Sbjct: 471 RHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQK 530

Query: 481 PGSATFPFFGVQPILVDEKGNELKGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFP 540
           PGSATFPFFGVQP++VDEKGNE++GECSGYLC+K SWP AFRTL+ D ERYETTYFKPF 
Sbjct: 531 PGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPFA 590

Query: 541 GYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHE 600
           GYYF+GDGC+RDKDGYYWLTGRVDDVINVSGHRIGTAEVESALV HPQCAEAAVVGIEHE
Sbjct: 591 GYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEAAVVGIEHE 650

Query: 601 VKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 660
           VKGQGIYAFVTL++GV YSEELRKSL+L VRNQIGAFAAPD+IHWAPGLPKTRSGKIMRR
Sbjct: 651 VKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRR 710

Query: 661 ILRKIASMQLDELGDTSTLADPDVVHHLISLAD 686
           ILRKIAS QL+ELGDTSTLADP VV  LI+LAD
Sbjct: 711 ILRKIASRQLEELGDTSTLADPSVVDQLIALAD 742

BLAST of Carg25318 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 142.5 bits (358), Expect = 1.3e-33
Identity = 137/516 (26.55%), Postives = 224/516 (43.41%), Query Frame = 0

Query: 148 SLTYTHLLNKVCQLASFLKAN-GVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFGG 207
           +LT+  L + + + AS L ++ G++ GD V +  P  +E  I  LA  R  A  + +   
Sbjct: 30  NLTHARLHDLIERAASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAA 89

Query: 208 FSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDVALTDSAQNGVSNESALKREST 267
           +++E     + D   K+++T    K G+                AQ   S        +T
Sbjct: 90  YTAEEFEFYLSDSDSKLLLTS---KEGNA--------------PAQEAASKLKISHVTAT 149

Query: 268 KWKEGRDIWWQDAVSTYPTTCTVEWVD-AEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTA 327
               G D+    A S        E V+  +D    L+TSG+T +PKGV  T    +   +
Sbjct: 150 LLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGVPLT---QLNLAS 209

Query: 328 TTFKYAFDYKLTDVYWCTADCGWITGHSYVTYGPLLNGASVVVFEGAPNYPDPGR----- 387
           +       YKLT+     +D   I    +  +G L    S +    A   P  GR     
Sbjct: 210 SVKNIKAVYKLTE-----SDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRFSATT 269

Query: 388 CWDIVDKYKVTIFYTAPTLVRSLM-REGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFD 447
            W  + KY  T +   PT+ + ++ R  +   T Y +  LR + S    + P       +
Sbjct: 270 FWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPK--LRFIRSCSASLAPVILSRLEE 329

Query: 448 VVGESRCPISDTWWQTETGGFMIT-PLPGAWPQKPGSATFPFFGVQPILVDEKGNELKGE 507
             G    P+ + +  TE    M + PLP   P KPGS   P  G +  +++EKG   +  
Sbjct: 330 AFG---APVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKP-VGQEMAILNEKGEIQEPN 389

Query: 508 CSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDV 567
             G +C++   P+  +   N+ E  +  +   F G++ TGD    D DGY  L GR+ ++
Sbjct: 390 NKGEVCIRG--PNVTKGYKNNPEANKAGF--EF-GWFHTGDIGYFDTDGYLHLVGRIKEL 449

Query: 568 INVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSL 627
           IN  G +I   EV++ L++HP  ++    G+  E  G+ I   V   +G + +EE  K+ 
Sbjct: 450 INRGGEKISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAF 506

Query: 628 ILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 655
               +  + AF  P ++     LPKT SGKI RRI+
Sbjct: 510 ---CKKNLAAFKVPKRVFITDNLPKTASGKIQRRIV 506

BLAST of Carg25318 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 137.5 bits (345), Expect = 4.0e-32
Identity = 122/513 (23.78%), Postives = 225/513 (43.86%), Query Frame = 0

Query: 148 SLTYTHLLNKVCQLASFLKANGVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFGGF 207
           +LT+T L   V  +A  L   G+RKG  V++  P  +  P+  L+   +GAI +      
Sbjct: 57  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 116

Query: 208 SSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDVALTDSAQNGVSNESALKRESTK 267
           +S  +A++I D  P +  T + +         K +  V + +   + V +   L     K
Sbjct: 117 TSNEIAKQIKDSNPVLAFTTSQLLPKISAA-AKKLPIVLMDEERVDSVGDVRRLVEMMKK 176

Query: 268 WKEGRDIWWQDAVSTYPTTCTVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATT 327
              G  +               E VD +D   LLY+SG+TG  KGV+ +    +    T 
Sbjct: 177 EPSGNRV--------------KERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTI 236

Query: 328 FKYAFDYKLTDVYWCTADCGWITGHSYVTYGPLLNGASVVVFEGAPNYPDPGRCWDIVDK 387
                       + CT     I G +    G L  G++++V      +         + K
Sbjct: 237 VNRFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMH----EMMSAIGK 296

Query: 388 YKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFDVVGESRCP 447
           Y+ T     P ++ +++   ++   +Y   S+  +   G P++      F +     +  
Sbjct: 297 YQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVK-- 356

Query: 448 ISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPILVDEKGNELKG-ECSGYLCLK 507
           I   +  TE+ G   +       ++ G+A      ++  +VD    ++ G + +G L LK
Sbjct: 357 ILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLK 416

Query: 508 SSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVINVSGHRI 567
              PS  +  ++++E   +T      G+  TGD C  D+DG+ ++  R+ ++I   G+++
Sbjct: 417 G--PSIMKGYFSNEEATSSTL--DSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQV 476

Query: 568 GTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRNQI 627
             AE+E+ L++HP+  +AAV+    +  GQ   A+V    G S SE   K+++  V  Q+
Sbjct: 477 APAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSE---KTIMEFVAKQV 536

Query: 628 GAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAS 660
             +    K+ +   +PK  SGKI+R+ L KIA+
Sbjct: 537 APYKRIRKVAFVSSIPKNPSGKILRKDLIKIAT 541

BLAST of Carg25318 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 136.7 bits (343), Expect = 6.9e-32
Identity = 128/522 (24.52%), Postives = 230/522 (44.06%), Query Frame = 0

Query: 149 LTYTHLLNKVCQLASFLKAN-GVRKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFGGF 208
           LT++ L   V ++A  L    G+R+GD V+I  P  + +P+  L+   +GA+ +      
Sbjct: 64  LTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLN 123

Query: 209 SSESLAQRIIDCKPKIVITCNAVKR----GSKVIHLKDIVDVALTDSAQ-NGVSNESALK 268
           +S  ++++I D  P +V T   +         V+   D V   LT + +  G+ +E   K
Sbjct: 124 TSGEISKQIADSNPTLVFTTRQLAPKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKK 183

Query: 269 RESTKWKEGRDIWWQDAVSTYPTTCTVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMV 328
             S +    R                   V+ +D   +LY+SG+TG  KGV+     +  
Sbjct: 184 EPSGQRVRDR-------------------VNQDDTAMMLYSSGTTGPSKGVI---SSHRN 243

Query: 329 YTATTFKYAFD-YKLTDVYWCTADCGWITGHSYVTYGPLLNGASVVVFEGAPNYPDPGRC 388
            TA   ++  D  K  D++ CT       G      G +  G++VV+      +      
Sbjct: 244 LTAHVARFISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHD----M 303

Query: 389 WDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFDVV 448
            D V+K++ T    AP ++ +++ + +    +Y   SL+ +   G P++      F +  
Sbjct: 304 MDAVEKHRATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKY 363

Query: 449 GESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPILVDEKGNELKG-ECS 508
                 I   +  TE+ G           ++ G+A      V+  +VD       G   +
Sbjct: 364 --PTVDILQGYALTESNGGGAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQT 423

Query: 509 GYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYYFTGDGCTRDKDGYYWLTGRVDDVIN 568
           G L LK   PS  +  + +QE    T      G+  TGD C  D+DG+ ++  R+ ++I 
Sbjct: 424 GELWLKG--PSISKGYFKNQEATNETI--NLEGWLKTGDLCYIDEDGFLFVVDRLKELIK 483

Query: 569 VSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLIL 628
             G+++  AE+E+ L++HP   +AAV+    +  GQ   A+V      + SE   K +I 
Sbjct: 484 YKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLSE---KQVID 543

Query: 629 TVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASMQL 663
            +  Q+  +    K+ +   +PKT SGK +R+ L K+A+ +L
Sbjct: 544 FISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLIKLATSKL 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022600.10.0e+00100.00Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal [Cucurbita argyrosperma ... [more]
XP_022928338.10.0e+0097.12acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal-like [Cucurbita moschata... [more]
XP_023529989.10.0e+0096.69acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal-like [Cucurbita pepo sub... [more]
KAG6588847.10.0e+0093.39Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal, partial [Cucurbita argy... [more]
XP_008449961.10.0e+0089.86PREDICTED: acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X1 [C... [more]
Match NameE-valueIdentityDescription
B9DGD60.0e+0078.21Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal OS=Arabidopsis thaliana ... [more]
Q9NR195.9e-23058.09Acetyl-coenzyme A synthetase, cytoplasmic OS=Homo sapiens OX=9606 GN=ACSS2 PE=1 ... [more]
Q9QXG47.2e-22857.45Acetyl-coenzyme A synthetase, cytoplasmic OS=Mus musculus OX=10090 GN=Acss2 PE=1... [more]
Q0AAW01.5e-22558.66Acetyl-coenzyme A synthetase OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 ... [more]
A0L5769.8e-22558.47Acetyl-coenzyme A synthetase OS=Magnetococcus marinus (strain ATCC BAA-1437 / JC... [more]
Match NameE-valueIdentityDescription
A0A6J1ENQ80.0e+0097.12Acetyl-coenzyme A synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435195 PE=3 ... [more]
A0A1S3BML70.0e+0089.86Acetate--CoA ligase OS=Cucumis melo OX=3656 GN=LOC103491691 PE=3 SV=1[more]
A0A1S3BP610.0e+0089.86Acetate--CoA ligase OS=Cucumis melo OX=3656 GN=LOC103491691 PE=3 SV=1[more]
A0A5A7U0070.0e+0089.71Acetate--CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold61G0... [more]
A0A0A0L7C40.0e+0089.28Acetate--CoA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G253500 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G36880.10.0e+0078.21acetyl-CoA synthetase [more]
AT5G36880.20.0e+0078.21acetyl-CoA synthetase [more]
AT3G48990.11.3e-3326.55AMP-dependent synthetase and ligase family protein [more]
AT1G20510.14.0e-3223.78OPC-8:0 CoA ligase1 [more]
AT1G20500.16.9e-3224.52AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.30coord: 557..685
e-value: 9.1E-32
score: 111.8
NoneNo IPR availablePANTHERPTHR24095:SF217ACETYL-COENZYME A SYNTHETASEcoord: 24..685
NoneNo IPR availablePANTHERPTHR24095ACETYL-COENZYME A SYNTHETASEcoord: 24..685
NoneNo IPR availableCDDcd05966ACScoord: 58..673
e-value: 0.0
score: 1138.83
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 59..679
IPR032387Acetyl-coenzyme A synthetase, N-terminal domainPFAMPF16177ACAS_Ncoord: 60..121
e-value: 6.3E-20
score: 70.8
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 42..556
e-value: 1.7E-202
score: 675.7
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 570..648
e-value: 5.2E-23
score: 81.8
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 131..561
e-value: 3.8E-96
score: 322.3
IPR011904Acetate-CoA ligaseTIGRFAMTIGR02188TIGR02188coord: 55..681
e-value: 1.5E-285
score: 946.4
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 300..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25318-RACarg25318-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019427 acetyl-CoA biosynthetic process from acetate
cellular_component GO:0009536 plastid
molecular_function GO:0003987 acetate-CoA ligase activity
molecular_function GO:0016208 AMP binding
molecular_function GO:0005524 ATP binding