Carg25178 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25178 vs. NCBI nr
Match: KAG7028394.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 706.8 bits (1823), Expect = 9.7e-200
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN
Sbjct: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180
           EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY
Sbjct: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180

Query: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSV 240
           LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSV
Sbjct: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSV 240

Query: 241 EKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKR 300
           EKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKR
Sbjct: 241 EKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKR 300

Query: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNR 360
           GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNR
Sbjct: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNR 360

Query: 361 PESEFDIDEPLLS 374
           PESEFDIDEPLLS
Sbjct: 361 PESEFDIDEPLLS 373

BLAST of Carg25178 vs. NCBI nr
Match: KAG6596863.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 668.3 bits (1723), Expect = 3.8e-188
Identity = 355/371 (95.69%), Postives = 361/371 (97.30%), Query Frame = 0

Query: 4   LGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWPT 63
           + I +  +  +FVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWPT
Sbjct: 5   IDIKARFIRSKFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWPT 64

Query: 64  KLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEKV 123
           KLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVAT+MPNLAPGLIFVIAWIFRLEKV
Sbjct: 65  KLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATSMPNLAPGLIFVIAWIFRLEKV 124

Query: 124 RLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAA--NVGRRPADQAFDEQKIVGCVYL 183
           RLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAA  NVGRR  DQAFDEQKIVGCVYL
Sbjct: 125 RLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANDNVGRRLTDQAFDEQKIVGCVYL 184

Query: 184 VSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVE 243
           VSAVV+LSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVE
Sbjct: 185 VSAVVILSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVE 244

Query: 244 KLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRG 303
           KLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRG
Sbjct: 245 KLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRG 304

Query: 304 PVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNRP 363
           PVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNRP
Sbjct: 305 PVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNRP 364

Query: 364 ESEFDIDEPLL 373
           ESEFDIDEPLL
Sbjct: 365 ESEFDIDEPLL 375

BLAST of Carg25178 vs. NCBI nr
Match: XP_023005578.1 (WAT1-related protein At5g47470-like [Cucurbita maxima])

HSP 1 Score: 618.2 bits (1593), Expect = 4.5e-173
Identity = 334/373 (89.54%), Postives = 338/373 (90.62%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MED GIMSGLV VQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVA+YFERSN
Sbjct: 1   MEDFGIMSGLVAVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVALYFERSN 60

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180
           EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY
Sbjct: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180

Query: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSV 240
           L+SAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLIT+VIHIALYQKLEIGWSYLSV
Sbjct: 181 LISAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITIVIHIALYQKLEIGWSYLSV 240

Query: 241 EKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKR 300
           EKLIAYCVL                                SGTVSGACVSFNGWAMKKR
Sbjct: 241 EKLIAYCVL--------------------------------SGTVSGACVSFNGWAMKKR 300

Query: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNR 360
           GPVLVSMFSPIGTVCSLVLSVFTLGEPI IGSLVGMFLMFTGLYFVLWAKRKEGYAN+NR
Sbjct: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPINIGSLVGMFLMFTGLYFVLWAKRKEGYANRNR 341

Query: 361 PESEFDIDEPLLS 374
           PESEFDIDEPLLS
Sbjct: 361 PESEFDIDEPLLS 341

BLAST of Carg25178 vs. NCBI nr
Match: XP_022937578.1 (WAT1-related protein At5g47470-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 613.6 bits (1581), Expect = 1.1e-171
Identity = 334/373 (89.54%), Postives = 336/373 (90.08%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MED GIMSGLV VQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFER N
Sbjct: 1   MEDFGIMSGLVAVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERLN 60

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180
           EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVG  PADQAFDEQKIVGCVY
Sbjct: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGWHPADQAFDEQKIVGCVY 180

Query: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSV 240
           LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSL+GVLITVVIHIALYQKLEIGWSYLSV
Sbjct: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLLGVLITVVIHIALYQKLEIGWSYLSV 240

Query: 241 EKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKR 300
           EKLIAYCVL                                SGTVSGACVSFNGWAMKKR
Sbjct: 241 EKLIAYCVL--------------------------------SGTVSGACVSFNGWAMKKR 300

Query: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNR 360
           GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYAN+NR
Sbjct: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANRNR 341

Query: 361 PESEFDIDEPLLS 374
           PESEFDIDEPLLS
Sbjct: 361 PESEFDIDEPLLS 341

BLAST of Carg25178 vs. NCBI nr
Match: XP_023540648.1 (WAT1-related protein At5g47470-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 608.6 bits (1568), Expect = 3.6e-170
Identity = 333/375 (88.80%), Postives = 337/375 (89.87%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MED GIMSGLV VQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN
Sbjct: 1   MEDFGIMSGLVAVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WPTKLSLKLI+QILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPTKLSLKLITQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAA--NVGRRPADQAFDEQKIVGC 180
           EKVRLSCIYSKIK+LGTFLCVIGAFTMSIMHSTLSDAAA  NVGRR  DQAFDEQKIVGC
Sbjct: 121 EKVRLSCIYSKIKLLGTFLCVIGAFTMSIMHSTLSDAAANDNVGRRLTDQAFDEQKIVGC 180

Query: 181 VYLVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYL 240
           VYLVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHI+LYQKLEIGWSYL
Sbjct: 181 VYLVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHISLYQKLEIGWSYL 240

Query: 241 SVEKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMK 300
           SVEKLIAYCVL                                SGTVSGACVSFNGWAMK
Sbjct: 241 SVEKLIAYCVL--------------------------------SGTVSGACVSFNGWAMK 300

Query: 301 KRGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQ 360
           KRGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYAN+
Sbjct: 301 KRGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANR 343

Query: 361 NRPESEFDIDEPLLS 374
           NRPESEFDIDEPLLS
Sbjct: 361 NRPESEFDIDEPLLS 343

BLAST of Carg25178 vs. ExPASy Swiss-Prot
Match: Q9FGL0 (WAT1-related protein At5g47470 OS=Arabidopsis thaliana OX=3702 GN=At5g47470 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 7.4e-102
Identity = 208/378 (55.03%), Postives = 262/378 (69.31%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           +E++ I+ GLV+VQFVYAGNS+ MS+LMSL + P T++IF+TF TF+I+SP A+ FER  
Sbjct: 25  IEEMVIVGGLVMVQFVYAGNSLLMSYLMSLGLGPFTIVIFSTFATFIILSPFAILFERKQ 84

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WP +LSL+LI +++LISF GVTLFQSLFL+GI+LTSP++ATAMPNLAPGLIF IAWI  L
Sbjct: 85  WPNELSLRLIGKLVLISFAGVTLFQSLFLEGIRLTSPAMATAMPNLAPGLIFFIAWIVGL 144

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQ----AFDEQKIV 180
           EK+ L C+YSK+KILGT LCV GA  MS+MHST      ++  +  D      FD  K+V
Sbjct: 145 EKMNLKCVYSKLKILGTLLCVFGALAMSVMHST------SISHKEEDDTPIFVFDRDKVV 204

Query: 181 GCVYLVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEI-GW 240
           GC+YL+ AV VLS+ VVLQASTL +FP P+SL AIT+L+GVLIT V+ +   +K ++   
Sbjct: 205 GCIYLLGAVFVLSTNVVLQASTLAEFPAPISLSAITALLGVLITTVVLLLQNRKTKVLAS 264

Query: 241 SYLSVEKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGW 300
           S +S   L+ Y VL                                +G VSGACVSFNGW
Sbjct: 265 SLISFGNLVGYSVL--------------------------------AGAVSGACVSFNGW 324

Query: 301 AMKKRGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGY 360
           AMKKRGPV VSMFSP  TV S+  +V TLGE + +GS+ GM LMF GLY VLWAK KEG+
Sbjct: 325 AMKKRGPVFVSMFSPFATVISVAFAVLTLGESVSLGSVGGMVLMFVGLYLVLWAKGKEGF 364

Query: 361 ANQNRPESEFDIDEPLLS 374
           +     ESEFD  +PLLS
Sbjct: 385 SEIESFESEFDSKKPLLS 364

BLAST of Carg25178 vs. ExPASy Swiss-Prot
Match: F4JMI7 (WAT1-related protein At4g16620 OS=Arabidopsis thaliana OX=3702 GN=At4g16620 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.5e-75
Identity = 169/382 (44.24%), Postives = 235/382 (61.52%), Query Frame = 0

Query: 3   DLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWP 62
           + GI+ GL   Q +YAGNS  +S L+SL IDPL ++I  TF + L+++P+A   ER  WP
Sbjct: 9   EAGIIGGLAGAQVIYAGNSELLSQLLSLGIDPLLIVILCTFASVLLITPLAFLLERKLWP 68

Query: 63  TKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEK 122
             LS KL  +++L++  GVTLFQ LFL+G++ TS S+ATAMPNL P  IFVIAW   +EK
Sbjct: 69  RSLSFKLKIKLVLVALAGVTLFQGLFLEGMKHTSASMATAMPNLCPAFIFVIAWAAGMEK 128

Query: 123 VRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLS--DAAANVGRRPADQAFDEQKIVGCVY 182
           V+LSC+YS++K+ GT LCV+GA  MS+MHST +   +   +   P +   D+ KI+GC+Y
Sbjct: 129 VKLSCMYSRVKMGGTVLCVMGALIMSLMHSTTATLSSVKTIPIVPDEVVVDKDKILGCLY 188

Query: 183 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIG-WSYLS 242
           L+ A+  LSS +VLQAS L +FP P+S+ ++ SL+G + TV +  AL   +E+G  S + 
Sbjct: 189 LLLAICGLSSSIVLQASILAEFPAPISMFSMVSLMGGITTVALQYALKGSMEMGSASVIG 248

Query: 243 VEKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKK 302
           +  L+ Y +L                                 G VSG  +SFN W +K+
Sbjct: 249 LGHLVGYAIL--------------------------------GGLVSGGGLSFNAWVIKR 308

Query: 303 RGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKE-----G 362
           +GPV+VS+FSPI TV  +V+S FT+ E   +GS  GM LMF GLYFVLWAK KE      
Sbjct: 309 KGPVIVSLFSPIATVVCVVVSAFTMEESFNLGSFAGMALMFGGLYFVLWAKGKEDCEEID 358

Query: 363 YANQNRPES----EFDIDEPLL 373
              Q+  ES    EFD+ +PLL
Sbjct: 369 EMKQDDEESLLRTEFDLQKPLL 358

BLAST of Carg25178 vs. ExPASy Swiss-Prot
Match: Q9M0B8 (WAT1-related protein At4g30420 OS=Arabidopsis thaliana OX=3702 GN=At4g30420 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 100/361 (27.70%), Postives = 172/361 (47.65%), Query Frame = 0

Query: 10  LVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN--WPTKLSL 69
           + ++Q  YAG ++F    +   + P   I++   F  + + P      R +    + L L
Sbjct: 3   MTMIQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPFLYLSRRKSKIAISSLDL 62

Query: 70  KLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEKVRLSC 129
           K  S I L+S  G+T+ Q+L+L+G+ LTS S+ +A+ N+ P + F+I+++   EK+ L  
Sbjct: 63  KSFSLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNLRD 122

Query: 130 IYSKIKILGTFLCVIGAFTMSIMHSTL---SDAAANVGRRPADQAFDEQK-IVGCVYLVS 189
           I    KI GT LCV GA +M+++       S++A  + +       D+   ++GC++L S
Sbjct: 123 IRGLAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLGHLKDQNTWLIGCLFLFS 182

Query: 190 AVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVEKL 249
           + +  S  ++LQ      +P  +SL A   L G +   V+   L +K    W        
Sbjct: 183 STLCWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFL-EKDPNAW-------- 242

Query: 250 IAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRGPV 309
                         IL S+     C+    +  +G       S    +   WA+ KRGPV
Sbjct: 243 --------------ILHSYSEFATCL----YAGIG------ASALSFTVQAWAIAKRGPV 302

Query: 310 LVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNRPES 365
             ++F+P+ TV   +L+     E I  GSL+G   +  GLY VLW K K+   NQ++ ++
Sbjct: 303 FSALFNPLCTVIVTILAALFFHEEIYTGSLIGGLGVILGLYTVLWGKAKDVMMNQDQRDN 330

BLAST of Carg25178 vs. ExPASy Swiss-Prot
Match: Q9FNA5 (WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.9e-26
Identity = 95/351 (27.07%), Postives = 172/351 (49.00%), Query Frame = 0

Query: 10  LVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWPTKLSLKL 69
           +V +Q +YA  S+     ++  + P  L+ +       +++P A+  ER+  P KL+ K+
Sbjct: 12  IVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRP-KLTFKI 71

Query: 70  ISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEKVRLSCIY 129
           + QI ++S     + Q+L+  G++LT+ +  +A+ N  P + F++A +F+LEKV +   +
Sbjct: 72  LLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERRH 131

Query: 130 SKIKILGTFLCVIGAFTMSIMHSTL------SDAAANVGRRPADQAFDEQKIV-GCVYLV 189
           S+ K++GT + + GA  M+ +   +      S++    G   A +   +  I  G + LV
Sbjct: 132 SQAKLVGTMVAIGGAMLMTFVKGNVIELPWTSNSRGLNGHTHAMRIPKQADIARGSIMLV 191

Query: 190 SAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVEK 249
           ++    S  ++LQA  L  +   +SL A+  ++G+L   V+ +   +K       +SV K
Sbjct: 192 ASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERK------NMSVWK 251

Query: 250 LIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRGP 309
           +     L++SI                             G VSG      GWA K+RGP
Sbjct: 252 INPDVTLLASIY---------------------------GGLVSGLAYYVIGWASKERGP 311

Query: 310 VLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKE 354
           V VS F+P+  V   +LS F   E + +G ++G  ++  G+Y VLW K K+
Sbjct: 312 VFVSAFNPLSMVLVAILSTFVFLEKVYVGRVIGSVVIVIGIYLVLWGKSKD 328

BLAST of Carg25178 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-25
Identity = 101/367 (27.52%), Postives = 170/367 (46.32%), Query Frame = 0

Query: 10  LVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWPTKLSLKL 69
           +V +QF YAG S+   F ++  + P  L  +      + ++P A + +R   P K++L +
Sbjct: 12  VVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRP-KMTLSI 71

Query: 70  ISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEKVRLSCIY 129
             +ILL+     T+ Q+L+  G++ TS +   AM N+ P   F++AWIFRLEKV +  I+
Sbjct: 72  FFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIH 131

Query: 130 SKIKILGTFLCVIGAFTMSIMHSTLSDA----AANVGRRPADQAFDEQKIVGCVYLVSAV 189
           S+ KILGT + V GA  M+++   L         ++ +  ++    +    G   +    
Sbjct: 132 SQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGASLIAIGC 191

Query: 190 VVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVEKLIA 249
           +  +  + LQA TL  +P  +SL A    +G + + ++ + + +     W+     KL+A
Sbjct: 192 ICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSKLLA 251

Query: 250 YCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRGPVLV 309
                             V+ G I                SG      G  MK RGPV V
Sbjct: 252 -----------------AVYGGVI---------------CSGIGYYVQGVIMKTRGPVFV 311

Query: 310 SMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNRPESEF 369
           + F+P+  V   +L    L E + +G ++G  ++  GLY VLW K K+       P S F
Sbjct: 312 TAFNPLSMVIVAILGSIILAEVMFLGRILGAIVIVLGLYSVLWGKSKD------EPSSSF 339

Query: 370 -DIDEPL 372
            D+D+ L
Sbjct: 372 SDMDKEL 339

BLAST of Carg25178 vs. ExPASy TrEMBL
Match: A0A6J1L2K3 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111498520 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.2e-173
Identity = 334/373 (89.54%), Postives = 338/373 (90.62%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MED GIMSGLV VQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVA+YFERSN
Sbjct: 1   MEDFGIMSGLVAVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVALYFERSN 60

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180
           EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY
Sbjct: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180

Query: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSV 240
           L+SAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLIT+VIHIALYQKLEIGWSYLSV
Sbjct: 181 LISAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITIVIHIALYQKLEIGWSYLSV 240

Query: 241 EKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKR 300
           EKLIAYCVL                                SGTVSGACVSFNGWAMKKR
Sbjct: 241 EKLIAYCVL--------------------------------SGTVSGACVSFNGWAMKKR 300

Query: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNR 360
           GPVLVSMFSPIGTVCSLVLSVFTLGEPI IGSLVGMFLMFTGLYFVLWAKRKEGYAN+NR
Sbjct: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPINIGSLVGMFLMFTGLYFVLWAKRKEGYANRNR 341

Query: 361 PESEFDIDEPLLS 374
           PESEFDIDEPLLS
Sbjct: 361 PESEFDIDEPLLS 341

BLAST of Carg25178 vs. ExPASy TrEMBL
Match: A0A6J1FGD7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111443917 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.4e-172
Identity = 334/373 (89.54%), Postives = 336/373 (90.08%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MED GIMSGLV VQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFER N
Sbjct: 1   MEDFGIMSGLVAVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERLN 60

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180
           EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVG  PADQAFDEQKIVGCVY
Sbjct: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGWHPADQAFDEQKIVGCVY 180

Query: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSV 240
           LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSL+GVLITVVIHIALYQKLEIGWSYLSV
Sbjct: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLLGVLITVVIHIALYQKLEIGWSYLSV 240

Query: 241 EKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKR 300
           EKLIAYCVL                                SGTVSGACVSFNGWAMKKR
Sbjct: 241 EKLIAYCVL--------------------------------SGTVSGACVSFNGWAMKKR 300

Query: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNR 360
           GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYAN+NR
Sbjct: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANRNR 341

Query: 361 PESEFDIDEPLLS 374
           PESEFDIDEPLLS
Sbjct: 361 PESEFDIDEPLLS 341

BLAST of Carg25178 vs. ExPASy TrEMBL
Match: A0A6J1FBU7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111443917 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.7e-165
Identity = 323/362 (89.23%), Postives = 325/362 (89.78%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MED GIMSGLV VQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFER N
Sbjct: 1   MEDFGIMSGLVAVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERLN 60

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQAFDEQKIVGCVY 180
           EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVG  PADQAFDEQKIVGCVY
Sbjct: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGWHPADQAFDEQKIVGCVY 180

Query: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSV 240
           LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSL+GVLITVVIHIALYQKLEIGWSYLSV
Sbjct: 181 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLLGVLITVVIHIALYQKLEIGWSYLSV 240

Query: 241 EKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKR 300
           EKLIAYCVL                                SGTVSGACVSFNGWAMKKR
Sbjct: 241 EKLIAYCVL--------------------------------SGTVSGACVSFNGWAMKKR 300

Query: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNR 360
           GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYAN+NR
Sbjct: 301 GPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANRNR 330

Query: 361 PE 363
           PE
Sbjct: 361 PE 330

BLAST of Carg25178 vs. ExPASy TrEMBL
Match: A0A5D3CP19 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00410 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 5.5e-132
Identity = 265/378 (70.11%), Postives = 298/378 (78.84%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MED  IMSGLV VQFVYAGNSVFMSFLMSL IDPLTL+IF+TF TFLIVSPVAVYFER +
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 60

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WP KLSLKLI Q++LISFGGVTLFQSL LKGIQLTSP++ATAMPNLAPGLIF IAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAA--NVGRRP--ADQAFDEQKIV 180
           EKV+LSCIYSKIKILGT LCVIGAFTMS+MHS  SD A   +V R+   ++  FDEQKI+
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFDEQKIL 180

Query: 181 GCVYLVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWS 240
           GC YL SA+++LSSVVVLQASTLGDFP PMSLCAITSL+GVL+T+ + I  Y +++I W 
Sbjct: 181 GCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAWP 240

Query: 241 YL-SVEKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGW 300
            L SV++L AY ++                                 GT+SGACVSFNGW
Sbjct: 241 LLISVKELAAYVIV--------------------------------GGTISGACVSFNGW 300

Query: 301 AMKKRGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGY 360
           AMKKRGPVLVSMFSPIGTVCSLVLSV TLGE I IGSL GMF+MFTGLYFVLWAK KE Y
Sbjct: 301 AMKKRGPVLVSMFSPIGTVCSLVLSVVTLGESINIGSLAGMFMMFTGLYFVLWAKGKERY 346

Query: 361 ANQNRPESEFDIDEPLLS 374
            + N  ESEFD+D+PLLS
Sbjct: 361 VSLNHLESEFDVDKPLLS 346

BLAST of Carg25178 vs. ExPASy TrEMBL
Match: A0A1S3BKN0 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103490881 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 5.5e-132
Identity = 265/378 (70.11%), Postives = 298/378 (78.84%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           MED  IMSGLV VQFVYAGNSVFMSFLMSL IDPLTL+IF+TF TFLIVSPVAVYFER +
Sbjct: 17  MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 76

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WP KLSLKLI Q++LISFGGVTLFQSL LKGIQLTSP++ATAMPNLAPGLIF IAWIFRL
Sbjct: 77  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 136

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAA--NVGRRP--ADQAFDEQKIV 180
           EKV+LSCIYSKIKILGT LCVIGAFTMS+MHS  SD A   +V R+   ++  FDEQKI+
Sbjct: 137 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFDEQKIL 196

Query: 181 GCVYLVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWS 240
           GC YL SA+++LSSVVVLQASTLGDFP PMSLCAITSL+GVL+T+ + I  Y +++I W 
Sbjct: 197 GCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAWP 256

Query: 241 YL-SVEKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGW 300
            L SV++L AY ++                                 GT+SGACVSFNGW
Sbjct: 257 LLISVKELAAYVIV--------------------------------GGTISGACVSFNGW 316

Query: 301 AMKKRGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGY 360
           AMKKRGPVLVSMFSPIGTVCSLVLSV TLGE I IGSL GMF+MFTGLYFVLWAK KE Y
Sbjct: 317 AMKKRGPVLVSMFSPIGTVCSLVLSVVTLGESINIGSLAGMFMMFTGLYFVLWAKGKERY 362

Query: 361 ANQNRPESEFDIDEPLLS 374
            + N  ESEFD+D+PLLS
Sbjct: 377 VSLNHLESEFDVDKPLLS 362

BLAST of Carg25178 vs. TAIR 10
Match: AT5G47470.1 (Nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 372.1 bits (954), Expect = 5.3e-103
Identity = 208/378 (55.03%), Postives = 262/378 (69.31%), Query Frame = 0

Query: 1   MEDLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN 60
           +E++ I+ GLV+VQFVYAGNS+ MS+LMSL + P T++IF+TF TF+I+SP A+ FER  
Sbjct: 25  IEEMVIVGGLVMVQFVYAGNSLLMSYLMSLGLGPFTIVIFSTFATFIILSPFAILFERKQ 84

Query: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120
           WP +LSL+LI +++LISF GVTLFQSLFL+GI+LTSP++ATAMPNLAPGLIF IAWI  L
Sbjct: 85  WPNELSLRLIGKLVLISFAGVTLFQSLFLEGIRLTSPAMATAMPNLAPGLIFFIAWIVGL 144

Query: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPADQ----AFDEQKIV 180
           EK+ L C+YSK+KILGT LCV GA  MS+MHST      ++  +  D      FD  K+V
Sbjct: 145 EKMNLKCVYSKLKILGTLLCVFGALAMSVMHST------SISHKEEDDTPIFVFDRDKVV 204

Query: 181 GCVYLVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEI-GW 240
           GC+YL+ AV VLS+ VVLQASTL +FP P+SL AIT+L+GVLIT V+ +   +K ++   
Sbjct: 205 GCIYLLGAVFVLSTNVVLQASTLAEFPAPISLSAITALLGVLITTVVLLLQNRKTKVLAS 264

Query: 241 SYLSVEKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGW 300
           S +S   L+ Y VL                                +G VSGACVSFNGW
Sbjct: 265 SLISFGNLVGYSVL--------------------------------AGAVSGACVSFNGW 324

Query: 301 AMKKRGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGY 360
           AMKKRGPV VSMFSP  TV S+  +V TLGE + +GS+ GM LMF GLY VLWAK KEG+
Sbjct: 325 AMKKRGPVFVSMFSPFATVISVAFAVLTLGESVSLGSVGGMVLMFVGLYLVLWAKGKEGF 364

Query: 361 ANQNRPESEFDIDEPLLS 374
           +     ESEFD  +PLLS
Sbjct: 385 SEIESFESEFDSKKPLLS 364

BLAST of Carg25178 vs. TAIR 10
Match: AT4G16620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.2e-76
Identity = 169/382 (44.24%), Postives = 235/382 (61.52%), Query Frame = 0

Query: 3   DLGIMSGLVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWP 62
           + GI+ GL   Q +YAGNS  +S L+SL IDPL ++I  TF + L+++P+A   ER  WP
Sbjct: 9   EAGIIGGLAGAQVIYAGNSELLSQLLSLGIDPLLIVILCTFASVLLITPLAFLLERKLWP 68

Query: 63  TKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEK 122
             LS KL  +++L++  GVTLFQ LFL+G++ TS S+ATAMPNL P  IFVIAW   +EK
Sbjct: 69  RSLSFKLKIKLVLVALAGVTLFQGLFLEGMKHTSASMATAMPNLCPAFIFVIAWAAGMEK 128

Query: 123 VRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLS--DAAANVGRRPADQAFDEQKIVGCVY 182
           V+LSC+YS++K+ GT LCV+GA  MS+MHST +   +   +   P +   D+ KI+GC+Y
Sbjct: 129 VKLSCMYSRVKMGGTVLCVMGALIMSLMHSTTATLSSVKTIPIVPDEVVVDKDKILGCLY 188

Query: 183 LVSAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIG-WSYLS 242
           L+ A+  LSS +VLQAS L +FP P+S+ ++ SL+G + TV +  AL   +E+G  S + 
Sbjct: 189 LLLAICGLSSSIVLQASILAEFPAPISMFSMVSLMGGITTVALQYALKGSMEMGSASVIG 248

Query: 243 VEKLIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKK 302
           +  L+ Y +L                                 G VSG  +SFN W +K+
Sbjct: 249 LGHLVGYAIL--------------------------------GGLVSGGGLSFNAWVIKR 308

Query: 303 RGPVLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKE-----G 362
           +GPV+VS+FSPI TV  +V+S FT+ E   +GS  GM LMF GLYFVLWAK KE      
Sbjct: 309 KGPVIVSLFSPIATVVCVVVSAFTMEESFNLGSFAGMALMFGGLYFVLWAKGKEDCEEID 358

Query: 363 YANQNRPES----EFDIDEPLL 373
              Q+  ES    EFD+ +PLL
Sbjct: 369 EMKQDDEESLLRTEFDLQKPLL 358

BLAST of Carg25178 vs. TAIR 10
Match: AT4G30420.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 122.5 bits (306), Expect = 7.3e-28
Identity = 100/361 (27.70%), Postives = 172/361 (47.65%), Query Frame = 0

Query: 10  LVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSN--WPTKLSL 69
           + ++Q  YAG ++F    +   + P   I++   F  + + P      R +    + L L
Sbjct: 3   MTMIQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPFLYLSRRKSKIAISSLDL 62

Query: 70  KLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEKVRLSC 129
           K  S I L+S  G+T+ Q+L+L+G+ LTS S+ +A+ N+ P + F+I+++   EK+ L  
Sbjct: 63  KSFSLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNLRD 122

Query: 130 IYSKIKILGTFLCVIGAFTMSIMHSTL---SDAAANVGRRPADQAFDEQK-IVGCVYLVS 189
           I    KI GT LCV GA +M+++       S++A  + +       D+   ++GC++L S
Sbjct: 123 IRGLAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLGHLKDQNTWLIGCLFLFS 182

Query: 190 AVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVEKL 249
           + +  S  ++LQ      +P  +SL A   L G +   V+   L +K    W        
Sbjct: 183 STLCWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFL-EKDPNAW-------- 242

Query: 250 IAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRGPV 309
                         IL S+     C+    +  +G       S    +   WA+ KRGPV
Sbjct: 243 --------------ILHSYSEFATCL----YAGIG------ASALSFTVQAWAIAKRGPV 302

Query: 310 LVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNRPES 365
             ++F+P+ TV   +L+     E I  GSL+G   +  GLY VLW K K+   NQ++ ++
Sbjct: 303 FSALFNPLCTVIVTILAALFFHEEIYTGSLIGGLGVILGLYTVLWGKAKDVMMNQDQRDN 330

BLAST of Carg25178 vs. TAIR 10
Match: AT5G13670.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 120.6 bits (301), Expect = 2.8e-27
Identity = 95/351 (27.07%), Postives = 172/351 (49.00%), Query Frame = 0

Query: 10  LVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWPTKLSLKL 69
           +V +Q +YA  S+     ++  + P  L+ +       +++P A+  ER+  P KL+ K+
Sbjct: 12  IVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRP-KLTFKI 71

Query: 70  ISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEKVRLSCIY 129
           + QI ++S     + Q+L+  G++LT+ +  +A+ N  P + F++A +F+LEKV +   +
Sbjct: 72  LLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERRH 131

Query: 130 SKIKILGTFLCVIGAFTMSIMHSTL------SDAAANVGRRPADQAFDEQKIV-GCVYLV 189
           S+ K++GT + + GA  M+ +   +      S++    G   A +   +  I  G + LV
Sbjct: 132 SQAKLVGTMVAIGGAMLMTFVKGNVIELPWTSNSRGLNGHTHAMRIPKQADIARGSIMLV 191

Query: 190 SAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVEK 249
           ++    S  ++LQA  L  +   +SL A+  ++G+L   V+ +   +K       +SV K
Sbjct: 192 ASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERK------NMSVWK 251

Query: 250 LIAYCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRGP 309
           +     L++SI                             G VSG      GWA K+RGP
Sbjct: 252 INPDVTLLASIY---------------------------GGLVSGLAYYVIGWASKERGP 311

Query: 310 VLVSMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKE 354
           V VS F+P+  V   +LS F   E + +G ++G  ++  G+Y VLW K K+
Sbjct: 312 VFVSAFNPLSMVLVAILSTFVFLEKVYVGRVIGSVVIVIGIYLVLWGKSKD 328

BLAST of Carg25178 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 119.0 bits (297), Expect = 8.1e-27
Identity = 101/367 (27.52%), Postives = 170/367 (46.32%), Query Frame = 0

Query: 10  LVIVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVAVYFERSNWPTKLSLKL 69
           +V +QF YAG S+   F ++  + P  L  +      + ++P A + +R   P K++L +
Sbjct: 12  VVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRP-KMTLSI 71

Query: 70  ISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRLEKVRLSCIY 129
             +ILL+     T+ Q+L+  G++ TS +   AM N+ P   F++AWIFRLEKV +  I+
Sbjct: 72  FFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIH 131

Query: 130 SKIKILGTFLCVIGAFTMSIMHSTLSDA----AANVGRRPADQAFDEQKIVGCVYLVSAV 189
           S+ KILGT + V GA  M+++   L         ++ +  ++    +    G   +    
Sbjct: 132 SQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGASLIAIGC 191

Query: 190 VVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITVVIHIALYQKLEIGWSYLSVEKLIA 249
           +  +  + LQA TL  +P  +SL A    +G + + ++ + + +     W+     KL+A
Sbjct: 192 ICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSKLLA 251

Query: 250 YCVLVSSIQFCGILRSFLVHIGCILMYVFTNVGDEKSGTVSGACVSFNGWAMKKRGPVLV 309
                             V+ G I                SG      G  MK RGPV V
Sbjct: 252 -----------------AVYGGVI---------------CSGIGYYVQGVIMKTRGPVFV 311

Query: 310 SMFSPIGTVCSLVLSVFTLGEPIKIGSLVGMFLMFTGLYFVLWAKRKEGYANQNRPESEF 369
           + F+P+  V   +L    L E + +G ++G  ++  GLY VLW K K+       P S F
Sbjct: 312 TAFNPLSMVIVAILGSIILAEVMFLGRILGAIVIVLGLYSVLWGKSKD------EPSSSF 339

Query: 370 -DIDEPL 372
            D+D+ L
Sbjct: 372 SDMDKEL 339