Carg25151 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25151 vs. NCBI nr
Match: KAG7035172.1 (Metal transporter Nramp3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.5e-288
Identity = 521/521 (100.00%), Postives = 521/521 (100.00%), Query Frame = 0

Query: 1   GFPTPISFSWTIMHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSW 60
           GFPTPISFSWTIMHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSW
Sbjct: 1   GFPTPISFSWTIMHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSW 60

Query: 61  RKLWLFTGPGFLMSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGV 120
           RKLWLFTGPGFLMSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGV
Sbjct: 61  RKLWLFTGPGFLMSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGV 120

Query: 121 ATGKHLAELCREEYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVI 180
           ATGKHLAELCREEYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVI
Sbjct: 121 ATGKHLAELCREEYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVI 180

Query: 181 ITALDCFIFLFLENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSS 240
           ITALDCFIFLFLENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSS
Sbjct: 181 ITALDCFIFLFLENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSS 240

Query: 241 KTIKQAVAVVGCVIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFII 300
           KTIKQAVAVVGCVIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFII
Sbjct: 241 KTIKQAVAVVGCVIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFII 300

Query: 301 NLFVTTVFAKAFYGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITG 360
           NLFVTTVFAKAFYGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITG
Sbjct: 301 NLFVTTVFAKAFYGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITG 360

Query: 361 TYAGQFIMGGFLNLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSI 420
           TYAGQFIMGGFLNLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSI
Sbjct: 361 TYAGQFIMGGFLNLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSI 420

Query: 421 QIPFALIPLLCLVSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLI 480
           QIPFALIPLLCLVSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLI
Sbjct: 421 QIPFALIPLLCLVSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLI 480

Query: 481 AIFVFVFIAAYVAFVVYLVYQSISFSSWNNINWKASTVDEN 522
           AIFVFVFIAAYVAFVVYLVYQSISFSSWNNINWKASTVDEN
Sbjct: 481 AIFVFVFIAAYVAFVVYLVYQSISFSSWNNINWKASTVDEN 521

BLAST of Carg25151 vs. NCBI nr
Match: XP_023533629.1 (metal transporter Nramp3-like [Cucurbita pepo subsp. pepo] >KAG6605195.1 Metal transporter Nramp3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 972.2 bits (2512), Expect = 1.7e-279
Identity = 508/509 (99.80%), Postives = 508/509 (99.80%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL
Sbjct: 301 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV
Sbjct: 421 ASKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 480

Query: 493 AFVVYLVYQSISFSSWNNINWKASTVDEN 522
           AFVVYLVYQSISFSSWNNINWKASTVDEN
Sbjct: 481 AFVVYLVYQSISFSSWNNINWKASTVDEN 509

BLAST of Carg25151 vs. NCBI nr
Match: XP_022947683.1 (metal transporter Nramp3 [Cucurbita moschata])

HSP 1 Score: 969.5 bits (2505), Expect = 1.1e-278
Identity = 507/509 (99.61%), Postives = 507/509 (99.61%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL
Sbjct: 301 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV
Sbjct: 421 ASKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 480

Query: 493 AFVVYLVYQSISFSSWNNINWKASTVDEN 522
           AFVVYLVYQSISFSSWNNINWKASTV EN
Sbjct: 481 AFVVYLVYQSISFSSWNNINWKASTVTEN 509

BLAST of Carg25151 vs. NCBI nr
Match: XP_023006983.1 (metal transporter Nramp3 [Cucurbita maxima])

HSP 1 Score: 954.5 bits (2466), Expect = 3.7e-274
Identity = 500/510 (98.04%), Postives = 504/510 (98.82%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDDHPQQRLLEEEEEETAYDL EKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDHPQQRLLEEEEEETAYDLAEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFA LIATMA+AFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAFLIATMAVAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL
Sbjct: 301 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLK+FSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVF+AAYV
Sbjct: 421 ASKEHLMGTFRIGPVLKIFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFVAAYV 480

Query: 493 AFVVYLVYQSISFSSWNN-INWKASTVDEN 522
           AFVVYLVYQSISFSSW+N INWK STV EN
Sbjct: 481 AFVVYLVYQSISFSSWHNFINWKVSTVVEN 510

BLAST of Carg25151 vs. NCBI nr
Match: KAA0032521.1 (metal transporter Nramp3-like [Cucumis melo var. makuwa])

HSP 1 Score: 889.8 bits (2298), Expect = 1.1e-254
Identity = 460/498 (92.37%), Postives = 482/498 (96.79%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDD  QQ L+EEEEEETAYDLTEKVVVIGIDE ESDSDVG+LPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGA  GYSLLWLL WATAMGLLIQLLSARLGVATG+HLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAE ALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFAVLIATMA++FAWMFGETKP+GKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           +IMPHNVFLHSALVQSRD++ +KKGRV+EALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGTD+ANSIGL+NAG+YL+DKYGGGL PILYIWAIGLLAAGQSSTITGTYAGQFIM GFL
Sbjct: 301 YGTDIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPT+IVALVFETSD MVD+LNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLK  SWLVAVLVMAINGYLLVSF SSEVNG+LIAIFVFVFIAAY+
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 493 AFVVYLVYQSISFSSWNN 511
           AFVVYLVY+SISF+SW+N
Sbjct: 481 AFVVYLVYRSISFTSWHN 498

BLAST of Carg25151 vs. ExPASy Swiss-Prot
Match: Q9SNV9 (Metal transporter Nramp3 OS=Arabidopsis thaliana OX=3702 GN=NRAMP3 PE=1 SV=2)

HSP 1 Score: 743.4 bits (1918), Expect = 1.7e-213
Identity = 375/489 (76.69%), Postives = 439/489 (89.78%), Query Frame = 0

Query: 25  EEEEEETAYDLTEKVVVIGIDEVESDSDVGM-----LPFSWRKLWLFTGPGFLMSIAFLD 84
           EEEEEETAYD TEKV ++  +E E D + G+      PFSW+KLWLFTGPGFLMSIAFLD
Sbjct: 14  EEEEEETAYDETEKVHIVR-NEEEDDLEHGVGCGGAPPFSWKKLWLFTGPGFLMSIAFLD 73

Query: 85  PGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCREEYPTWAR 144
           PGNLE +LQAGA+ GYSLLWLL WATAMGLL+QLLSARLGVATG+HLAELCR+EYPTWAR
Sbjct: 74  PGNLEGDLQAGAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRHLAELCRDEYPTWAR 133

Query: 145 IVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRK 204
           +VLWVMAELALIG+DIQEVIGSAIAIKILSNG LPLWAGV+ITALDCF+FLFLENYG+RK
Sbjct: 134 MVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAGVVITALDCFVFLFLENYGIRK 193

Query: 205 LEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGCVIMPHNV 264
           LEAVFAVLIATM ++FAWMFG+ KP+G ELL+GIL+PKLSS+TI++AV VVGC+IMPHNV
Sbjct: 194 LEAVFAVLIATMGVSFAWMFGQAKPSGSELLIGILVPKLSSRTIQKAVGVVGCIIMPHNV 253

Query: 265 FLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDMAN 324
           FLHSALVQSR+VD  +K RV+EAL YY+IEST+ALF+SF+INLFVTTVFAK FY TD+AN
Sbjct: 254 FLHSALVQSREVDKRQKYRVQEALNYYTIESTIALFISFLINLFVTTVFAKGFYNTDLAN 313

Query: 325 SIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNLKLKKW 384
           SIGL+NAG+YLQ+KYGGG+ PILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLN K+KKW
Sbjct: 314 SIGLVNAGQYLQEKYGGGVFPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNFKMKKW 373

Query: 385 LRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCLVSKEHLM 444
           LRALITRS AI+PTIIVALVF++S+  +D+LNEWLNVLQSIQIPFALIPLLCLVSKE +M
Sbjct: 374 LRALITRSCAIIPTIIVALVFDSSEATLDVLNEWLNVLQSIQIPFALIPLLCLVSKEQIM 433

Query: 445 GTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYVAFVVYLV 504
           G+F+IGP+ K  +WLVA LV+ INGYLL+ F S+EV+G++   FV +F A+Y AF++YL+
Sbjct: 434 GSFKIGPLYKTIAWLVAALVIMINGYLLLEFFSNEVSGIVYTGFVTLFTASYGAFILYLI 493

Query: 505 YQSISFSSW 509
            + I+F+ W
Sbjct: 494 ARGITFTPW 501

BLAST of Carg25151 vs. ExPASy Swiss-Prot
Match: Q9FN18 (Metal transporter Nramp4 OS=Arabidopsis thaliana OX=3702 GN=NRAMP4 PE=2 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 6.7e-210
Identity = 368/484 (76.03%), Postives = 433/484 (89.46%), Query Frame = 0

Query: 29  EETAYDLTEKVVVIGIDEVES---DSDVGMLP-FSWRKLWLFTGPGFLMSIAFLDPGNLE 88
           EE AY+ TEKV+++GIDE E    D D G  P FSW+KLWLFTGPGFLMSIAFLDPGNLE
Sbjct: 14  EERAYEETEKVLIVGIDEEEDADYDDDPGNSPKFSWKKLWLFTGPGFLMSIAFLDPGNLE 73

Query: 89  SNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCREEYPTWARIVLWV 148
           S+LQAGAI GYSL+WLL WATA+GLLIQLLSARLGVATG+HLAELCREEYPTWAR+VLW+
Sbjct: 74  SDLQAGAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRHLAELCREEYPTWARMVLWI 133

Query: 149 MAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRKLEAVF 208
           MAE+ALIGADIQEVIGSAIAIKILSNG +PLWAGV+ITALDCFIFLFLENYG+RKLEAVF
Sbjct: 134 MAEIALIGADIQEVIGSAIAIKILSNGLVPLWAGVVITALDCFIFLFLENYGIRKLEAVF 193

Query: 209 AVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGCVIMPHNVFLHSA 268
           A+LIATMA+AFAWMFG+TKP+G ELL+G L+PKLSS+TIKQAV +VGC+IMPHNVFLHSA
Sbjct: 194 AILIATMALAFAWMFGQTKPSGTELLVGALVPKLSSRTIKQAVGIVGCIIMPHNVFLHSA 253

Query: 269 LVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDMANSIGLI 328
           LVQSR+VD  K+ RV+EAL+YYSIEST AL VSFIIN+FVTTVFAK+FYGT++A++IGL 
Sbjct: 254 LVQSREVDPKKRFRVKEALKYYSIESTGALAVSFIINVFVTTVFAKSFYGTEIADTIGLA 313

Query: 329 NAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNLKLKKWLRALI 388
           NAG+YLQDKYGGG  PILYIWAIG+LAAGQSSTITGTYAGQFIMGGFLNLK+KKW+RALI
Sbjct: 314 NAGQYLQDKYGGGFFPILYIWAIGVLAAGQSSTITGTYAGQFIMGGFLNLKMKKWVRALI 373

Query: 389 TRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCLVSKEHLMGTFRI 448
           TRS AI+PT+IVALVF++SD M+D LNEWLNVLQS+QIPFA+IPLLCLVS E +MG+F+I
Sbjct: 374 TRSCAIIPTMIVALVFDSSDSMLDELNEWLNVLQSVQIPFAVIPLLCLVSNEQIMGSFKI 433

Query: 449 GPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYVAFVVYLVYQSIS 508
            P+++  SW+VA LV+AINGYL+V F S     L++ + V +F  AYV FV+YL+ + ++
Sbjct: 434 QPLVQTISWIVAALVIAINGYLMVDFFSGAATNLILLVPVIIFAIAYVVFVLYLISRGLT 493

BLAST of Carg25151 vs. ExPASy Swiss-Prot
Match: Q9C6B2 (Metal transporter Nramp2 OS=Arabidopsis thaliana OX=3702 GN=NRAMP2 PE=2 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.5e-204
Identity = 368/508 (72.44%), Postives = 430/508 (84.65%), Query Frame = 0

Query: 15  PDDHPQQRL-LEEEEEETAYDLTEKVVVIGIDEVESDSDVGML--PFSWRKLWLFTGPGF 74
           P   P Q L   + E E A++  EK++++   E   D   G    PFSWRKLWLFTGPGF
Sbjct: 18  PPPPPSQSLPSTDSESEAAFETNEKILIVDF-ESPDDPTTGDTPPPFSWRKLWLFTGPGF 77

Query: 75  LMSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCR 134
           LMSIAFLDPGNLE +LQAGAI GYSLLWLL WATAMGLLIQ+LSAR+GVATG+HLAELCR
Sbjct: 78  LMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRHLAELCR 137

Query: 135 EEYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLF 194
           +EYPTWAR VLW MAELALIGADIQEVIGSAIAI+ILS G LPLWAGV+ITA DCF+FLF
Sbjct: 138 DEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLPLWAGVVITASDCFLFLF 197

Query: 195 LENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVG 254
           LENYGVRKLEAVFAVLIATM ++FAWMFGETKP+GKEL++GIL+P+LSSKTI+QAV VVG
Sbjct: 198 LENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGILLPRLSSKTIRQAVGVVG 257

Query: 255 CVIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKA 314
           CVIMPHNVFLHSALVQSR +D  +K RV+EAL YY IES++ALF+SF+INLFVTTVFAK 
Sbjct: 258 CVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESSVALFISFMINLFVTTVFAKG 317

Query: 315 FYGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGF 374
           FYGT+ AN+IGL+NAG+YLQ+K+GGGLLPILYIW IGLLAAGQSSTITGTYAGQFIMGGF
Sbjct: 318 FYGTEKANNIGLVNAGQYLQEKFGGGLLPILYIWGIGLLAAGQSSTITGTYAGQFIMGGF 377

Query: 375 LNLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLC 434
           LNL+LKKW+RA+ITRS AIVPT+IVA+VF TS+  +D+LNEWLNVLQS+QIPFAL+PLL 
Sbjct: 378 LNLRLKKWMRAVITRSCAIVPTMIVAIVFNTSEASLDVLNEWLNVLQSVQIPFALLPLLT 437

Query: 435 LVSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAY 494
           LVSKE +MG F+IGP+L+  +W VA LVM INGYLL+ F  SEV+G L  + V V+  AY
Sbjct: 438 LVSKEEIMGDFKIGPILQRIAWTVAALVMIINGYLLLDFFVSEVDGFLFGVTVCVWTTAY 497

Query: 495 VAFVVYLVYQSISFSSWNNINWKASTVD 520
           +AF+VYL+  S  F S     W +S+++
Sbjct: 498 IAFIVYLISHSNFFPS----PWSSSSIE 520

BLAST of Carg25151 vs. ExPASy Swiss-Prot
Match: Q2QN30 (Metal transporter Nramp6 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP6 PE=2 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 6.8e-194
Identity = 352/487 (72.28%), Postives = 411/487 (84.39%), Query Frame = 0

Query: 25  EEEEEETAYDLTEKVVVIGIDEVESDSD------------VGMLPFSWRKLWLFTGPGFL 84
           +++ EE AY+ TEKV+V   D  ++D D             G+ PFSWRKLWLFTGPGFL
Sbjct: 37  DDDLEERAYEATEKVIVSISDFPDADDDEEESGLATSTAASGIPPFSWRKLWLFTGPGFL 96

Query: 85  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 144
           MSIAFLDPGNLE +LQAGA+ G +LLWLL WAT+MGLL+QLL+AR+GVATG+HLAELCR+
Sbjct: 97  MSIAFLDPGNLEGDLQAGAVAGDTLLWLLLWATSMGLLVQLLAARVGVATGRHLAELCRD 156

Query: 145 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 204
           EYP+WAR  LW+MAE+A++GADIQEVIGSAIAIKILS G LPLWAGV+ITALDCFIFL L
Sbjct: 157 EYPSWARRALWLMAEVAMVGADIQEVIGSAIAIKILSRGFLPLWAGVVITALDCFIFLSL 216

Query: 205 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 264
           ENYGVRKLEAVFA+LIATMA++FAWMF +TKPN K L +GIL+PKLSS+TI+QAV VVGC
Sbjct: 217 ENYGVRKLEAVFAILIATMAVSFAWMFTDTKPNMKNLFIGILVPKLSSRTIRQAVGVVGC 276

Query: 265 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 324
           VIMPHNVFLHSALVQSR +D  K+ +VREALRYYSIEST+AL VSF+INLFVTTVFAK F
Sbjct: 277 VIMPHNVFLHSALVQSRKIDPNKEHQVREALRYYSIESTIALAVSFMINLFVTTVFAKGF 336

Query: 325 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 384
           YGT  A +IGL NAG+YLQ+K+GGG  PILYIW IGLLAAGQSSTITGTYAGQFIMGGFL
Sbjct: 337 YGTKEAGNIGLENAGQYLQEKFGGGFFPILYIWGIGLLAAGQSSTITGTYAGQFIMGGFL 396

Query: 385 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 444
           NLKLKKW+R+LITRSFAIVPTIIVAL F+ SD + D+LNEWLNVLQSIQIPFALIPL+ L
Sbjct: 397 NLKLKKWIRSLITRSFAIVPTIIVALFFDKSDSL-DVLNEWLNVLQSIQIPFALIPLITL 456

Query: 445 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 500
           VSKE +MG F+IG   +  +W VA L++ INGYLL+ F SSE+ GLL    + V + AY 
Sbjct: 457 VSKEKVMGVFKIGRNTQAVTWTVATLLITINGYLLLDFFSSEIRGLLSGSILCVAVLAYA 516

BLAST of Carg25151 vs. ExPASy Swiss-Prot
Match: Q10Q65 (Metal transporter Nramp2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP2 PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 1.9e-188
Identity = 342/487 (70.23%), Postives = 399/487 (81.93%), Query Frame = 0

Query: 26  EEEEETAYDLTEKVVVIGIDEVESDSDVG----MLP-FSWRKLWLFTGPGFLMSIAFLDP 85
           +E +E AYD  +KV +   D    D   G    M P FSWRKLW FTGPGFLM IAFLDP
Sbjct: 28  DEYDERAYDSDDKVSIAVSDSDSEDGGGGGGDAMRPAFSWRKLWRFTGPGFLMCIAFLDP 87

Query: 86  GNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCREEYPTWARI 145
           GNLE +LQAGA  GY LLWLL WAT MG L+QLLSARLGVATGKHLAELCREEYP WA  
Sbjct: 88  GNLEGDLQAGAAAGYQLLWLLLWATVMGALVQLLSARLGVATGKHLAELCREEYPPWATR 147

Query: 146 VLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRKL 205
            LW M ELAL+GADIQEVIGSAIAIKILS G +PLW GV+ITA DCFIFLFLENYGVRKL
Sbjct: 148 ALWAMTELALVGADIQEVIGSAIAIKILSAGTVPLWGGVVITAFDCFIFLFLENYGVRKL 207

Query: 206 EAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGCVIMPHNVF 265
           EA F VLIA MA++FA MFGETKP+GKELL+G+++PKLSS+TIKQAV +VGC+IMPHNVF
Sbjct: 208 EAFFGVLIAVMAVSFAIMFGETKPSGKELLIGLVVPKLSSRTIKQAVGIVGCIIMPHNVF 267

Query: 266 LHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDMANS 325
           LHSALVQSR +D+ KK RV+EA+ YY+IES LAL VSF IN+ VTTVFAK FYG++ A+ 
Sbjct: 268 LHSALVQSRKIDTNKKSRVQEAVFYYNIESILALIVSFFINICVTTVFAKGFYGSEQADG 327

Query: 326 IGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNLKLKKWL 385
           IGL NAG+YLQ KYG    PILYIWAIGLLA+GQSSTITGTYAGQF+MGGFLNL+LKKWL
Sbjct: 328 IGLENAGQYLQQKYGTAFFPILYIWAIGLLASGQSSTITGTYAGQFVMGGFLNLRLKKWL 387

Query: 386 RALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCLVSKEHLMG 445
           RA+ITRSFAI+PT+IVAL F+T D  +DILNE LNVLQSIQIPFALIPL+ LVSKE +MG
Sbjct: 388 RAMITRSFAIIPTMIVALFFDTEDPTMDILNEALNVLQSIQIPFALIPLITLVSKEQVMG 447

Query: 446 TFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYVAFVVYLVY 505
           +F +GP+ K+ SW+V V +M INGYL++SF ++EV G L+   + V +A Y+AF+VYL+ 
Sbjct: 448 SFVVGPITKVISWIVTVFLMLINGYLILSFYATEVRGALVRSSLCVVLAVYLAFIVYLIM 507

Query: 506 QSISFSS 508
           ++ S  S
Sbjct: 508 RNTSLYS 514

BLAST of Carg25151 vs. ExPASy TrEMBL
Match: A0A6J1G7A3 (metal transporter Nramp3 OS=Cucurbita moschata OX=3662 GN=LOC111451475 PE=3 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 5.4e-279
Identity = 507/509 (99.61%), Postives = 507/509 (99.61%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL
Sbjct: 301 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV
Sbjct: 421 ASKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 480

Query: 493 AFVVYLVYQSISFSSWNNINWKASTVDEN 522
           AFVVYLVYQSISFSSWNNINWKASTV EN
Sbjct: 481 AFVVYLVYQSISFSSWNNINWKASTVTEN 509

BLAST of Carg25151 vs. ExPASy TrEMBL
Match: A0A6J1L6G3 (metal transporter Nramp3 OS=Cucurbita maxima OX=3661 GN=LOC111499613 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.8e-274
Identity = 500/510 (98.04%), Postives = 504/510 (98.82%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDDHPQQRLLEEEEEETAYDL EKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDHPQQRLLEEEEEETAYDLAEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFA LIATMA+AFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAFLIATMAVAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL
Sbjct: 301 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLK+FSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVF+AAYV
Sbjct: 421 ASKEHLMGTFRIGPVLKIFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFVAAYV 480

Query: 493 AFVVYLVYQSISFSSWNN-INWKASTVDEN 522
           AFVVYLVYQSISFSSW+N INWK STV EN
Sbjct: 481 AFVVYLVYQSISFSSWHNFINWKVSTVVEN 510

BLAST of Carg25151 vs. ExPASy TrEMBL
Match: A0A5A7SSI5 (Metal transporter Nramp3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00300 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 5.5e-255
Identity = 460/498 (92.37%), Postives = 482/498 (96.79%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDD  QQ L+EEEEEETAYDLTEKVVVIGIDE ESDSDVG+LPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGA  GYSLLWLL WATAMGLLIQLLSARLGVATG+HLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAE ALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFAVLIATMA++FAWMFGETKP+GKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           +IMPHNVFLHSALVQSRD++ +KKGRV+EALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGTD+ANSIGL+NAG+YL+DKYGGGL PILYIWAIGLLAAGQSSTITGTYAGQFIM GFL
Sbjct: 301 YGTDIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPT+IVALVFETSD MVD+LNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLK  SWLVAVLVMAINGYLLVSF SSEVNG+LIAIFVFVFIAAY+
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 493 AFVVYLVYQSISFSSWNN 511
           AFVVYLVY+SISF+SW+N
Sbjct: 481 AFVVYLVYRSISFTSWHN 498

BLAST of Carg25151 vs. ExPASy TrEMBL
Match: A0A5D3BH32 (Metal transporter Nramp3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00750 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.1e-254
Identity = 459/498 (92.17%), Postives = 482/498 (96.79%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDD  QQ L+EEEEEETAYDLTEKVVVIGIDE ESDSDVG+LPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGA  GYSLLWLL WATAMGLLIQLLSARLGVATG+HLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAE ALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFAVLIATMA++FAWMFGETKP+GKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           +IMPHNVFLHSALVQSRD++ +KKGRV+EALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGT++ANSIGL+NAG+YL+DKYGGGL PILYIWAIGLLAAGQSSTITGTYAGQFIM GFL
Sbjct: 301 YGTNIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPT+IVALVFETSD MVD+LNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLK  SWLVAVLVMAINGYLLVSF SSEVNG+LIAIFVFVFIAAY+
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 493 AFVVYLVYQSISFSSWNN 511
           AFVVYLVY+SISF+SW+N
Sbjct: 481 AFVVYLVYRSISFTSWHN 498

BLAST of Carg25151 vs. ExPASy TrEMBL
Match: A0A1S3C1X3 (metal transporter Nramp3-like OS=Cucumis melo OX=3656 GN=LOC103495920 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.1e-254
Identity = 459/498 (92.17%), Postives = 482/498 (96.79%), Query Frame = 0

Query: 13  MHPDDHPQQRLLEEEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFL 72
           MHPDD  QQ L+EEEEEETAYDLTEKVVVIGIDE ESDSDVG+LPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 73  MSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCRE 132
           MSIAFLDPGNLESNLQAGA  GYSLLWLL WATAMGLLIQLLSARLGVATG+HLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 133 EYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 192
           EYPTWARIVLWVMAE ALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 193 ENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGC 252
           ENYGVRKLEAVFAVLIATMA++FAWMFGETKP+GKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 253 VIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 312
           +IMPHNVFLHSALVQSRD++ +KKGRV+EALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 313 YGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 372
           YGT++ANSIGL+NAG+YL+DKYGGGL PILYIWAIGLLAAGQSSTITGTYAGQFIM GFL
Sbjct: 301 YGTNIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 373 NLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCL 432
           NLKLKKWLRALITRSFAIVPT+IVALVFETSD MVD+LNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 433 VSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYV 492
            SKEHLMGTFRIGPVLK  SWLVAVLVMAINGYLLVSF SSEVNG+LIAIFVFVFIAAY+
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 493 AFVVYLVYQSISFSSWNN 511
           AFVVYLVY+SISF+SW+N
Sbjct: 481 AFVVYLVYRSISFTSWHN 498

BLAST of Carg25151 vs. TAIR 10
Match: AT2G23150.1 (natural resistance-associated macrophage protein 3 )

HSP 1 Score: 743.4 bits (1918), Expect = 1.2e-214
Identity = 375/489 (76.69%), Postives = 439/489 (89.78%), Query Frame = 0

Query: 25  EEEEEETAYDLTEKVVVIGIDEVESDSDVGM-----LPFSWRKLWLFTGPGFLMSIAFLD 84
           EEEEEETAYD TEKV ++  +E E D + G+      PFSW+KLWLFTGPGFLMSIAFLD
Sbjct: 14  EEEEEETAYDETEKVHIVR-NEEEDDLEHGVGCGGAPPFSWKKLWLFTGPGFLMSIAFLD 73

Query: 85  PGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCREEYPTWAR 144
           PGNLE +LQAGA+ GYSLLWLL WATAMGLL+QLLSARLGVATG+HLAELCR+EYPTWAR
Sbjct: 74  PGNLEGDLQAGAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRHLAELCRDEYPTWAR 133

Query: 145 IVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRK 204
           +VLWVMAELALIG+DIQEVIGSAIAIKILSNG LPLWAGV+ITALDCF+FLFLENYG+RK
Sbjct: 134 MVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAGVVITALDCFVFLFLENYGIRK 193

Query: 205 LEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGCVIMPHNV 264
           LEAVFAVLIATM ++FAWMFG+ KP+G ELL+GIL+PKLSS+TI++AV VVGC+IMPHNV
Sbjct: 194 LEAVFAVLIATMGVSFAWMFGQAKPSGSELLIGILVPKLSSRTIQKAVGVVGCIIMPHNV 253

Query: 265 FLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDMAN 324
           FLHSALVQSR+VD  +K RV+EAL YY+IEST+ALF+SF+INLFVTTVFAK FY TD+AN
Sbjct: 254 FLHSALVQSREVDKRQKYRVQEALNYYTIESTIALFISFLINLFVTTVFAKGFYNTDLAN 313

Query: 325 SIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNLKLKKW 384
           SIGL+NAG+YLQ+KYGGG+ PILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLN K+KKW
Sbjct: 314 SIGLVNAGQYLQEKYGGGVFPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNFKMKKW 373

Query: 385 LRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCLVSKEHLM 444
           LRALITRS AI+PTIIVALVF++S+  +D+LNEWLNVLQSIQIPFALIPLLCLVSKE +M
Sbjct: 374 LRALITRSCAIIPTIIVALVFDSSEATLDVLNEWLNVLQSIQIPFALIPLLCLVSKEQIM 433

Query: 445 GTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYVAFVVYLV 504
           G+F+IGP+ K  +WLVA LV+ INGYLL+ F S+EV+G++   FV +F A+Y AF++YL+
Sbjct: 434 GSFKIGPLYKTIAWLVAALVIMINGYLLLEFFSNEVSGIVYTGFVTLFTASYGAFILYLI 493

Query: 505 YQSISFSSW 509
            + I+F+ W
Sbjct: 494 ARGITFTPW 501

BLAST of Carg25151 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 731.5 bits (1887), Expect = 4.8e-211
Identity = 368/484 (76.03%), Postives = 433/484 (89.46%), Query Frame = 0

Query: 29  EETAYDLTEKVVVIGIDEVES---DSDVGMLP-FSWRKLWLFTGPGFLMSIAFLDPGNLE 88
           EE AY+ TEKV+++GIDE E    D D G  P FSW+KLWLFTGPGFLMSIAFLDPGNLE
Sbjct: 14  EERAYEETEKVLIVGIDEEEDADYDDDPGNSPKFSWKKLWLFTGPGFLMSIAFLDPGNLE 73

Query: 89  SNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCREEYPTWARIVLWV 148
           S+LQAGAI GYSL+WLL WATA+GLLIQLLSARLGVATG+HLAELCREEYPTWAR+VLW+
Sbjct: 74  SDLQAGAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRHLAELCREEYPTWARMVLWI 133

Query: 149 MAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRKLEAVF 208
           MAE+ALIGADIQEVIGSAIAIKILSNG +PLWAGV+ITALDCFIFLFLENYG+RKLEAVF
Sbjct: 134 MAEIALIGADIQEVIGSAIAIKILSNGLVPLWAGVVITALDCFIFLFLENYGIRKLEAVF 193

Query: 209 AVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGCVIMPHNVFLHSA 268
           A+LIATMA+AFAWMFG+TKP+G ELL+G L+PKLSS+TIKQAV +VGC+IMPHNVFLHSA
Sbjct: 194 AILIATMALAFAWMFGQTKPSGTELLVGALVPKLSSRTIKQAVGIVGCIIMPHNVFLHSA 253

Query: 269 LVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDMANSIGLI 328
           LVQSR+VD  K+ RV+EAL+YYSIEST AL VSFIIN+FVTTVFAK+FYGT++A++IGL 
Sbjct: 254 LVQSREVDPKKRFRVKEALKYYSIESTGALAVSFIINVFVTTVFAKSFYGTEIADTIGLA 313

Query: 329 NAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNLKLKKWLRALI 388
           NAG+YLQDKYGGG  PILYIWAIG+LAAGQSSTITGTYAGQFIMGGFLNLK+KKW+RALI
Sbjct: 314 NAGQYLQDKYGGGFFPILYIWAIGVLAAGQSSTITGTYAGQFIMGGFLNLKMKKWVRALI 373

Query: 389 TRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCLVSKEHLMGTFRI 448
           TRS AI+PT+IVALVF++SD M+D LNEWLNVLQS+QIPFA+IPLLCLVS E +MG+F+I
Sbjct: 374 TRSCAIIPTMIVALVFDSSDSMLDELNEWLNVLQSVQIPFAVIPLLCLVSNEQIMGSFKI 433

Query: 449 GPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYVAFVVYLVYQSIS 508
            P+++  SW+VA LV+AINGYL+V F S     L++ + V +F  AYV FV+YL+ + ++
Sbjct: 434 QPLVQTISWIVAALVIAINGYLMVDFFSGAATNLILLVPVIIFAIAYVVFVLYLISRGLT 493

BLAST of Carg25151 vs. TAIR 10
Match: AT1G47240.1 (NRAMP metal ion transporter 2 )

HSP 1 Score: 713.8 bits (1841), Expect = 1.0e-205
Identity = 368/508 (72.44%), Postives = 430/508 (84.65%), Query Frame = 0

Query: 15  PDDHPQQRL-LEEEEEETAYDLTEKVVVIGIDEVESDSDVGML--PFSWRKLWLFTGPGF 74
           P   P Q L   + E E A++  EK++++   E   D   G    PFSWRKLWLFTGPGF
Sbjct: 18  PPPPPSQSLPSTDSESEAAFETNEKILIVDF-ESPDDPTTGDTPPPFSWRKLWLFTGPGF 77

Query: 75  LMSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCR 134
           LMSIAFLDPGNLE +LQAGAI GYSLLWLL WATAMGLLIQ+LSAR+GVATG+HLAELCR
Sbjct: 78  LMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRHLAELCR 137

Query: 135 EEYPTWARIVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLF 194
           +EYPTWAR VLW MAELALIGADIQEVIGSAIAI+ILS G LPLWAGV+ITA DCF+FLF
Sbjct: 138 DEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLPLWAGVVITASDCFLFLF 197

Query: 195 LENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVG 254
           LENYGVRKLEAVFAVLIATM ++FAWMFGETKP+GKEL++GIL+P+LSSKTI+QAV VVG
Sbjct: 198 LENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGILLPRLSSKTIRQAVGVVG 257

Query: 255 CVIMPHNVFLHSALVQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKA 314
           CVIMPHNVFLHSALVQSR +D  +K RV+EAL YY IES++ALF+SF+INLFVTTVFAK 
Sbjct: 258 CVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESSVALFISFMINLFVTTVFAKG 317

Query: 315 FYGTDMANSIGLINAGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGF 374
           FYGT+ AN+IGL+NAG+YLQ+K+GGGLLPILYIW IGLLAAGQSSTITGTYAGQFIMGGF
Sbjct: 318 FYGTEKANNIGLVNAGQYLQEKFGGGLLPILYIWGIGLLAAGQSSTITGTYAGQFIMGGF 377

Query: 375 LNLKLKKWLRALITRSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLC 434
           LNL+LKKW+RA+ITRS AIVPT+IVA+VF TS+  +D+LNEWLNVLQS+QIPFAL+PLL 
Sbjct: 378 LNLRLKKWMRAVITRSCAIVPTMIVAIVFNTSEASLDVLNEWLNVLQSVQIPFALLPLLT 437

Query: 435 LVSKEHLMGTFRIGPVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAY 494
           LVSKE +MG F+IGP+L+  +W VA LVM INGYLL+ F  SEV+G L  + V V+  AY
Sbjct: 438 LVSKEEIMGDFKIGPILQRIAWTVAALVMIINGYLLLDFFVSEVDGFLFGVTVCVWTTAY 497

Query: 495 VAFVVYLVYQSISFSSWNNINWKASTVD 520
           +AF+VYL+  S  F S     W +S+++
Sbjct: 498 IAFIVYLISHSNFFPS----PWSSSSIE 520

BLAST of Carg25151 vs. TAIR 10
Match: AT4G18790.1 (NRAMP metal ion transporter family protein )

HSP 1 Score: 647.5 bits (1669), Expect = 9.1e-186
Identity = 324/494 (65.59%), Postives = 402/494 (81.38%), Query Frame = 0

Query: 26  EEEEETAYDLTEKVVVIGIDEVESDSDVGMLPFSWRKLWLFTGPGFLMSIAFLDPGNLES 85
           + EE+  ++   +  ++ ++E    +   + PFSW KLW FTGPGFLMSIAFLDPGN+E 
Sbjct: 33  QPEEDELHESPPENQILNVEEDRDKTYDSVPPFSWAKLWKFTGPGFLMSIAFLDPGNIEG 92

Query: 86  NLQAGAIGGYSLLWLLFWATAMGLLIQLLSARLGVATGKHLAELCREEYPTWARIVLWVM 145
           +LQAGA+ GYSLLWLL WAT MGLL+QLLSAR+GVATG+HLAE+CR EYP+WARI+LW M
Sbjct: 93  DLQAGAVAGYSLLWLLLWATLMGLLMQLLSARIGVATGRHLAEICRSEYPSWARILLWFM 152

Query: 146 AELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRKLEAVFA 205
           AE+ALIGADIQEVIGSAIA++IL+ G LP+W GVIIT+ DCF+  +LE  G+RKLE +FA
Sbjct: 153 AEVALIGADIQEVIGSAIALQILTRGFLPIWVGVIITSFDCFLISYLEKCGMRKLEGLFA 212

Query: 206 VLIATMAIAFAWMFGETKPNGKELLLGILIPKLSSKTIKQAVAVVGCVIMPHNVFLHSAL 265
           VLIATMA++FAWMF ETKP+ +EL +GI+IPKL SKTI++AV VVGCVI PHNVFLHSAL
Sbjct: 213 VLIATMALSFAWMFNETKPSVEELFIGIIIPKLGSKTIREAVGVVGCVITPHNVFLHSAL 272

Query: 266 VQSRDVDSTKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDMANSIGLIN 325
           VQSR  D  +  RV+EAL YY+IES+ ALFVSF+INLFVT VFAK FYGT  A+SIGL+N
Sbjct: 273 VQSRKTDPKEINRVQEALNYYTIESSAALFVSFMINLFVTAVFAKGFYGTKQADSIGLVN 332

Query: 326 AGEYLQDKYGGGLLPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNLKLKKWLRALIT 385
           AG YLQ+KYGGG+ PILYIW IGLLAAGQSSTITGTYAGQFIM GFL+L++++WL A IT
Sbjct: 333 AGYYLQEKYGGGVFPILYIWGIGLLAAGQSSTITGTYAGQFIMEGFLDLQMEQWLSAFIT 392

Query: 386 RSFAIVPTIIVALVFETSDGMVDILNEWLNVLQSIQIPFALIPLLCLVSKEHLMGTFRIG 445
           RSFAIVPT+ VA++F TS+G +D+LNEWLN+LQS+QIPFA+IPLL +VS EH+MG F+IG
Sbjct: 393 RSFAIVPTMFVAIMFNTSEGSLDVLNEWLNILQSMQIPFAVIPLLTMVSNEHIMGVFKIG 452

Query: 446 PVLKMFSWLVAVLVMAINGYLLVSFISSEVNGLLIAIFVFVFIAAYVAFVVYLV-YQSIS 505
           P L+  +W VAV VM INGYLL+ F  +EV G L+   VF  +  Y++F++YLV Y+S  
Sbjct: 453 PSLEKLAWTVAVFVMMINGYLLLDFFMAEVEGFLVGFLVFGGVVGYISFIIYLVSYRSSQ 512

Query: 506 FSSWNNINWKASTV 519
            SSW+++      V
Sbjct: 513 SSSWSSLEMSERVV 526

BLAST of Carg25151 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 318.2 bits (814), Expect = 1.3e-86
Identity = 201/478 (42.05%), Postives = 300/478 (62.76%), Query Frame = 0

Query: 49  SDSDVGMLP--FSWRKLWLFTGPGFLMSIAFLDPGNLESNLQAGAIGGYSLLWLLFWATA 108
           SDS+  ++P   SW+  + + GPGFL+SIA++DPGN E++LQ+GA   Y LLW++  A+ 
Sbjct: 22  SDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSGAQYKYELLWIILVASC 81

Query: 109 MGLLIQLLSARLGVATGKHLAELCREEYPTWARIVLWVMAELALIGADIQEVIGSAIAIK 168
             L+IQ L+A LGV TGKHLAE CR EY      +LWV+AE+A++  DI EVIG+A A+ 
Sbjct: 82  AALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAVVACDIPEVIGTAFALN 141

Query: 169 ILSNGALPLWAGVIITALDCFIFLFLENYGVRKLEAVFAVLIATMAIAFAWMFGETKPNG 228
           +L N  +P+W GV++T L   I L L+ YG+RKLE + A L+ T+A+ F      +KP+ 
Sbjct: 142 MLFN--IPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIALCFFVELHYSKPDP 201

Query: 229 KELLLGILIPKLSSK-TIKQAVAVVGCVIMPHNVFLHSALVQSRDVDSTKKGRVREALRY 288
           KE+L G+ +P+L        A++++G ++MPHN+FLHSALV SR +  +  G ++EA RY
Sbjct: 202 KEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVTG-IKEACRY 261

Query: 289 YSIESTLALFVSFIINLFVTTVFAKAFYGTDMA-------NSIGLINAGEYLQDKYGGGL 348
           Y IES LAL V+F+IN+ V +V       +D++         + L  A   L++  G   
Sbjct: 262 YLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDLDLNKASFLLRNVVGKWS 321

Query: 349 LPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFLNLKLKKWLRALITRSFAIVPTIIVAL 408
                ++AI LLA+GQSSTITGTYAGQ++M GFL+L+L+ WLR  +TR  AI+P++IVAL
Sbjct: 322 SK---LFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCLAIIPSLIVAL 381

Query: 409 VFETSD-GMVDILNEWLNVLQSIQIPFALIPLLCLVSKEHLMGTFRIGPVLKMFSWLVAV 468
           +  ++  G + I+    +++ S ++PFAL+PLL   S +  MG+     V+   +W++  
Sbjct: 382 IGGSAGAGKLIII---ASMILSFELPFALVPLLKFTSSKTKMGSHANSLVISSVTWIIGG 441

Query: 469 LVMAINGYLLVS-----FISSEVNGLLI---AIFVFVFIAAYVAFVVYLVYQSISFSS 508
           L+M IN Y LVS      + S +N + I    +  F  IA Y+A + YLV +    SS
Sbjct: 442 LIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRKNRESS 490