Carg25121 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25121
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	SNF1-related protein kinase regulatory subunit beta-2-like
Location	Carg_Chr13: 5424817 .. 5428153 (+)
RNA-Seq Expression	Carg25121
Synteny	Carg25121

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTAGTGAGTAGGTGGAAGAGCTCTTTTTCTAATCAGTCCCAACTTCAATGATGATCTTGCTTCTAGTGGAGGGCGATAAAATGGGCAAGAAAACAAGAACGATCTCACTTTCTTCTCCTTTTCTCTGCCTTAATTCTCGCTTTAGCACTCGTGTGGGGTGAACCCTGAAGCCTCAAGATTACAGCCATCTCCCCAGAAACGGAATACTCATTCAGTGATGGAGGAGTTGGTGTAGAGGCCCCCATCAATGGCCTTCTTCGTCCATTCAATCAATTCGAACGCCGCTCCATTTCAGTGATCATGTGAGGACGACAATCCCCAATTTCTTCTCCCAACGCAGCTGCCTTGAGCCGCCTCTGATTCTTCCCCAAGTCATCGGAGAATTTTGTTGAAGATAAGGGGTTTAGCGATTCTCCTTCGACATCGATTTCTGTTGAGCGGATTTTTGATTTCTGGGGTTTCTTTTTGTCGTCTATGATTTTTTATTTCCCTTGCTCTAATCTGATTTTGAGATTCTGGAAGTGGGTTGTGTGTTTTGTTTTCTTGTTCTTAGTTCTTTAAGGGCTTTGGGTTGTATTTGTACTGATTAATCAATGGGTAACGTGAATGGGAGAGAGTATGTAGACGAAGAAGACGATGATGATGAAAATGAAGAAGAAGTTGAAGAAGAGGGTTTAATTGTGCACGAGGGCGTCGCCGTGGCTGATGGCGCTCATATTCTATACCGCCCGCAAGGTGTTTGTCAAAAGTTGTTGGGCGGCCAGTCCCCTCCACAAAGCCCCACGGCCACTCGATCGCCATTGATTTTCACTCCTCAAGTAAGCCCTCTCTCTCTCTTTATCTTGAGAAGTTCCTTTTAGTTTCTTGTAGCTAATTGCCTATGCTTACTAGCAATGTGATGAATCTGAATTAATGTATTACTTGGATTTTGAATTTCATTTGGAGATTGATATCACCTCTTGTAGATATAACTTGTATGACAATGGATAAATGTTAGTTTTGATCTTCAAGTTTGATACAGAGATTTGTTGTTGGCTGAAAAGAAGTGGGATAAGTTTGAGATTACGGGGGTTAGTGTTGAGGATGGTTGGGAGGGAGTCCTGCCTCGGCTAATTAAGGGTTGATCATGGGTTTATAAGCAATGAATACTATCTTCATTGGTATGAGACCTTTGGGGAAATCAAAAGCAAAACCACGAGAGCTTATGCTCAAAGTGGATAATTTCGTACCATTGTGGATAGAGTCCCACGTTGGCTAATAAAGGGGTTGATCATAGGTTTATAATTAAGGAACACTATCTCTACTGGTATGGGGCCTTTTGAGAAATCAAAAGCAAAACCATGAGAGCTTATTTCAAAGCAGACAATATCGTACCATTATGGAAGTCTGTGATTTCTAATAGTTAGTTGAAGGCTTTGCCTACTTTTTTTTTTTTTTCGAGAAGTTGGTTTAAGGCAGTTACTTTTGCTTAAACTTTATATAATGGGGTTCATTCATTTTTTTTTTCCTTCAATATATATACATTGGGTGTATCTAGCTGCTGGTTCAGCTTAGAGCTGACAAGAACATGTTCAATTTCAAGAGACATAAAGTTACTTCACACTTGTCAAACTTTAACAAATTCATGTTACAGAGAGAACAAGAGGAGTTGATTGAAATGGTCCTATGTTTCATTCTCAGTTGGATAGGTTGTTGTTTACTATCATCTTTTATTAGTTCCTTAATGTTTCTTTTACACTTTTATCCTCAGGTTCCGCTCACTCCCCTACGAAAACCTGATGAGATGCTCGTAATTAGCCAATCCCAAATGATGCAAGGTTCCTTGGGATATGAAGAAACGTGCGAACAAAGCATTCCAACCATGATTACATGGAGTTATGGAGGAAAGGAAGTAGCTATTGAGGGATCATGGGATCTATGGAGAACAAGGTTTTCTTCTCTTTTTGTACCATTCCACAATTATCGTTTCTGAACTGCTGCCGAAATATCTGATACTAGTTGTACCTCATTAACCAGAACACCCTTGCAGAGATCAGGGAGAGACTTCACTATAATGAAAGTGCTTCCTGCTGGGGTTTATCAATATAGGTTTCTCGTGGACGGACAATGGAGGTATTCGCCGGAGTTGCCTTGGGGCCAAGATGACGTTGGTAACGCTTACAATGTTTTGGACTTGCAGGTAACTGAAAACGTAGGATATGCAAGGAAAAGGGTAGAGTTAATCGACTTGGAGATAAACTCGTGTTGTTTCGTAAACAACCATTAAATATTTTAGCCTACTTCAATGCTTTTTCCCTATGAGGCCCGAAACTTGGTTTGTCTAACTAACAATCAATAGTCCATCGACATAGACAATCTATCGTTGTATGTCAATTGCTTTTTTTCCTTTTATATTCCGCTTGTTCTTCATTATTGGTGTTGGTGTTACTATTCTCTGTTAGAAATCACGACTCTTCATAATGGTATGATATGTCCACTTTGAGTATAAGCTCTCATGGTTTTGCTATTGGTTTCCCCATAGCTGAGACCTTACATCAGTTCATCAATTATGCAGGATAACGTGCCTGAAGATATCGAAAGCATCTCTAGCTTCGAGCCTCCTCAGTCACCAGATTCGAGCTATGACAATTTACTACTCGGGTCTGATGACTACGCGAAAGAACCGCCATTGGTTCCCCCACACCTACAGAAGACACTGCTCGATTCGCCATCATCATACATGGAGATTCCGCCCTGTCTGTCAAGGCCACAACATGTAGTATTGAATCATCTTTACATGCAGAGAGGGAAGAGCGGTCCATCGATTGTTGCACTCGGTACGACACATCGGTTTCTAACCAAGTATGTAACTGTTGTGCTCTACAAATCTTTCCAAAGGTAAGTAAATCTCTTACTTATCTTATTGTCTCTGCTGTTCTGGGTTATCTGTAATCATAAATTTTGACATGCCAATGCCAAGTTAGTCGAAGCAAACCAAGTTTGTGCTTCAGAAACCTCTAAACCCACACTGCCATCTTGGTTTGATTTGTTCTTCACCAGTTGACACTCATTTTAGAAAGATTTGGTTTACTGTTTGCTTTCTCTGCCATTATGGAATTTGAGTTTATGTTAGCTGATTATGTAATGGTTTATATAAATGTAGTCTTGATTCCTTAACATACACCAACCAATCTGTTTTGTTAGCCTAAGTGTCACGATCAGGGTTGACGTCTTTGTCAACCGACTCGGACAAGGAAGACCAGGACATCCGCCATGCGGCCATAGGCAACATGTCTTGGACAAGTATGACCGTGACACTAAACATCCCCTCAAATTCTTCGACTCAAATTGGAGATT

mRNA sequence

ACTAGTGAGTAGGTGGAAGAGCTCTTTTTCTAATCAGTCCCAACTTCAATGATGATCTTGCTTCTAGTGGAGGGCGATAAAATGGGCAAGAAAACAAGAACGATCTCACTTTCTTCTCCTTTTCTCTGCCTTAATTCTCGCTTTAGCACTCGTGTGGGGTGAACCCTGAAGCCTCAAGATTACAGCCATCTCCCCAGAAACGGAATACTCATTCAGTGATGGAGGAGTTGGTGTAGAGGCCCCCATCAATGGCCTTCTTCGTCCATTCAATCAATTCGAACGCCGCTCCATTTCAGTGATCATGTGAGGACGACAATCCCCAATTTCTTCTCCCAACGCAGCTGCCTTGAGCCGCCTCTGATTCTTCCCCAAGTCATCGGAGAATTTTGTTGAAGATAAGGGGTTTAGCGATTCTCCTTCGACATCGATTTCTGTTGAGCGGATTTTTGATTTCTGGGGTTTCTTTTTGTCGTCTATGATTTTTTATTTCCCTTGCTCTAATCTGATTTTGAGATTCTGGAAGTGGGTTGTGTGTTTTGTTTTCTTGTTCTTAGTTCTTTAAGGGCTTTGGGTTGTATTTGTACTGATTAATCAATGGGTAACGTGAATGGGAGAGAGTATGTAGACGAAGAAGACGATGATGATGAAAATGAAGAAGAAGTTGAAGAAGAGGGTTTAATTGTGCACGAGGGCGTCGCCGTGGCTGATGGCGCTCATATTCTATACCGCCCGCAAGGTGTTTGTCAAAAGTTGTTGGGCGGCCAGTCCCCTCCACAAAGCCCCACGGCCACTCGATCGCCATTGATTTTCACTCCTCAAGTTCCGCTCACTCCCCTACGAAAACCTGATGAGATGCTCGTAATTAGCCAATCCCAAATGATGCAAGGTTCCTTGGGATATGAAGAAACGTGCGAACAAAGCATTCCAACCATGATTACATGGAGTTATGGAGGAAAGGAAGTAGCTATTGAGGGATCATGGGATCTATGGAGAACAAGAACACCCTTGCAGAGATCAGGGAGAGACTTCACTATAATGAAAGTGCTTCCTGCTGGGGTTTATCAATATAGGTTTCTCGTGGACGGACAATGGAGGTATTCGCCGGAGTTGCCTTGGGGCCAAGATGACGTTGGTAACGCTTACAATGTTTTGGACTTGCAGGATAACGTGCCTGAAGATATCGAAAGCATCTCTAGCTTCGAGCCTCCTCAGTCACCAGATTCGAGCTATGACAATTTACTACTCGGGTCTGATGACTACGCGAAAGAACCGCCATTGGTTCCCCCACACCTACAGAAGACACTGCTCGATTCGCCATCATCATACATGGAGATTCCGCCCTGTCTGTCAAGGCCACAACATGTAGTATTGAATCATCTTTACATGCAGAGAGGGAAGAGCGGTCCATCGATTGTTGCACTCGGTACGACACATCGGTTTCTAACCAAGTATGTAACTGTTGTGCTCTACAAATCTTTCCAAAGGTAAGTAAATCTCTTACTTATCTTATTGTCTCTGCTGTTCTGGGTTATCTGTAATCATAAATTTTGACATGCCAATGCCAAGTTAGTCGAAGCAAACCAAGTTTGTGCTTCAGAAACCTCTAAACCCACACTGCCATCTTGGTTTGATTTGTTCTTCACCAGTTGACACTCATTTTAGAAAGATTTGGTTTACTGTTTGCTTTCTCTGCCATTATGGAATTTGAGTTTATGTTAGCTGATTATGTAATGGTTTATATAAATGTAGTCTTGATTCCTTAACATACACCAACCAATCTGTTTTGTTAGCCTAAGTGTCACGATCAGGGTTGACGTCTTTGTCAACCGACTCGGACAAGGAAGACCAGGACATCCGCCATGCGGCCATAGGCAACATGTCTTGGACAAGTATGACCGTGACACTAAACATCCCCTCAAATTCTTCGACTCAAATTGGAGATT

Coding sequence (CDS)

ATGGGTAACGTGAATGGGAGAGAGTATGTAGACGAAGAAGACGATGATGATGAAAATGAAGAAGAAGTTGAAGAAGAGGGTTTAATTGTGCACGAGGGCGTCGCCGTGGCTGATGGCGCTCATATTCTATACCGCCCGCAAGGTGTTTGTCAAAAGTTGTTGGGCGGCCAGTCCCCTCCACAAAGCCCCACGGCCACTCGATCGCCATTGATTTTCACTCCTCAAGTTCCGCTCACTCCCCTACGAAAACCTGATGAGATGCTCGTAATTAGCCAATCCCAAATGATGCAAGGTTCCTTGGGATATGAAGAAACGTGCGAACAAAGCATTCCAACCATGATTACATGGAGTTATGGAGGAAAGGAAGTAGCTATTGAGGGATCATGGGATCTATGGAGAACAAGAACACCCTTGCAGAGATCAGGGAGAGACTTCACTATAATGAAAGTGCTTCCTGCTGGGGTTTATCAATATAGGTTTCTCGTGGACGGACAATGGAGGTATTCGCCGGAGTTGCCTTGGGGCCAAGATGACGTTGGTAACGCTTACAATGTTTTGGACTTGCAGGATAACGTGCCTGAAGATATCGAAAGCATCTCTAGCTTCGAGCCTCCTCAGTCACCAGATTCGAGCTATGACAATTTACTACTCGGGTCTGATGACTACGCGAAAGAACCGCCATTGGTTCCCCCACACCTACAGAAGACACTGCTCGATTCGCCATCATCATACATGGAGATTCCGCCCTGTCTGTCAAGGCCACAACATGTAGTATTGAATCATCTTTACATGCAGAGAGGGAAGAGCGGTCCATCGATTGTTGCACTCGGTACGACACATCGGTTTCTAACCAAGTATGTAACTGTTGTGCTCTACAAATCTTTCCAAAGGTAA

Protein sequence

MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR

Homology

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BLAST of Carg25121 vs. NCBI nr
Match: XP_022927294.1 (SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita moschata] >KAG6583747.1 SNF1-related protein kinase regulatory subunit beta-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019388.1 SNF1-related protein kinase regulatory subunit beta-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 606.3 bits (1562), Expect = 1.4e-169
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60
           MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP
Sbjct: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60

Query: 61  QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120
           QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG
Sbjct: 61  QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120

Query: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180
           KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG
Sbjct: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180

Query: 181 NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240
           NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS
Sbjct: 181 NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240

Query: 241 PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 298
           PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR
Sbjct: 241 PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 297

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BLAST of Carg25121 vs. NCBI nr
Match: XP_023520032.1 (SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.0 bits (1543), Expect = 2.3e-167
Identity = 295/297 (99.33%), Postives = 296/297 (99.66%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60
           MGNVNGREYVDEE DDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP
Sbjct: 1   MGNVNGREYVDEE-DDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60

Query: 61  QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120
           QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITW+YGG
Sbjct: 61  QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWTYGG 120

Query: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180
           KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG
Sbjct: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180

Query: 181 NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240
           NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS
Sbjct: 181 NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240

Query: 241 PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 298
           PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR
Sbjct: 241 PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 296

[Close]

BLAST of Carg25121 vs. NCBI nr
Match: XP_023001594.1 (SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita maxima])

HSP 1 Score: 577.8 bits (1488), Expect = 5.4e-161
Identity = 285/297 (95.96%), Postives = 290/297 (97.64%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60
           MGNVNGREYVDEEDDD+   EEVEEEGLIV+EGVAVADGAHILYRPQGVCQKL+GGQSPP
Sbjct: 1   MGNVNGREYVDEEDDDE--NEEVEEEGLIVNEGVAVADGAHILYRPQGVCQKLMGGQSPP 60

Query: 61  QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120
           QSPTA RSPLIFTPQVPLTPLRKP+EMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG
Sbjct: 61  QSPTAPRSPLIFTPQVPLTPLRKPEEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120

Query: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180
           KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG
Sbjct: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180

Query: 181 NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240
           NAYNVLDLQDNVPEDI+SIS FEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS
Sbjct: 181 NAYNVLDLQDNVPEDIKSISCFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240

Query: 241 PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 298
           PSSYME PP LSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR
Sbjct: 241 PSSYMETPPSLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 295

[Close]

BLAST of Carg25121 vs. NCBI nr
Match: XP_038895464.1 (SNF1-related protein kinase regulatory subunit beta-2-like [Benincasa hispida])

HSP 1 Score: 515.4 bits (1326), Expect = 3.3e-142
Identity = 259/305 (84.92%), Postives = 276/305 (90.49%), Query Frame = 0


Query: 1   MGNVNGREYVDEED----DDDENEEEV---EEEGLIVHEGVAVADGAHILYRPQGVCQKL 60
           MGNVNGREYVDE      ++DENEEEV   +E+ LIVHEGVA+AD AHI   PQGVC KL
Sbjct: 1   MGNVNGREYVDESPSGSVEEDENEEEVVEEQEDALIVHEGVAMADEAHIQCHPQGVCSKL 60

Query: 61  LGGQSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETC-EQSIPT 120
           +GGQSPP SPTA RSPLIFTPQVPLTPLRK DEML++S S  MQ SLGYEETC EQSI T
Sbjct: 61  MGGQSPPHSPTAPRSPLIFTPQVPLTPLRKADEMLILSHSP-MQSSLGYEETCNEQSIAT 120

Query: 121 MITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPEL 180
           MITWSYGGKEVAIEGSWD WRTR PLQRSG+DFT+MKVLPAGVYQYRFLVDGQWRY+PEL
Sbjct: 121 MITWSYGGKEVAIEGSWDRWRTRMPLQRSGKDFTLMKVLPAGVYQYRFLVDGQWRYAPEL 180

Query: 181 PWGQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPH 240
           PW QDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGS++YAKEPPLVPPH
Sbjct: 181 PWAQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSNEYAKEPPLVPPH 240

Query: 241 LQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLY 298
           LQ+TLL+SP+SYMEIP CLSRPQHVVLNHLYMQRGK GPSIVALGTTHRFL+KYVTVVLY
Sbjct: 241 LQRTLLNSPASYMEIPTCLSRPQHVVLNHLYMQRGKGGPSIVALGTTHRFLSKYVTVVLY 300

[Close]

BLAST of Carg25121 vs. NCBI nr
Match: XP_008463594.1 (PREDICTED: SNF1-related protein kinase regulatory subunit beta-2 isoform X2 [Cucumis melo])

HSP 1 Score: 514.2 bits (1323), Expect = 7.4e-142
Identity = 259/305 (84.92%), Postives = 276/305 (90.49%), Query Frame = 0


Query: 1   MGNVNGREYVDEE-----DDDDENEEEVEEE--GLIVHEGVAVADGAHILYRPQGVCQKL 60
           MGNVNGREYVDE      +++DENEEEVEEE  GLIVHEGV +ADGA IL    GVC KL
Sbjct: 1   MGNVNGREYVDESPSGSVEENDENEEEVEEEEDGLIVHEGVPMADGAPILCHSLGVCSKL 60

Query: 61  LGGQSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETC-EQSIPT 120
           + GQSPPQSPTATRSPLIFTPQVPLTPLRK DEML+ + SQ MQ SLG+E+TC EQSIPT
Sbjct: 61  MRGQSPPQSPTATRSPLIFTPQVPLTPLRKADEMLIHAHSQ-MQSSLGFEDTCNEQSIPT 120

Query: 121 MITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPEL 180
           MITWSYGGKEVAIEGSWD WRTR PLQRSG+DFT+MKVLPAGVYQYRF VDGQWRY+PEL
Sbjct: 121 MITWSYGGKEVAIEGSWDHWRTRMPLQRSGKDFTLMKVLPAGVYQYRFFVDGQWRYAPEL 180

Query: 181 PWGQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPH 240
           PW QDD GNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPH
Sbjct: 181 PWAQDDSGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPH 240

Query: 241 LQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLY 298
           LQ+TLL+SPS+YMEIP CLSRPQHVVLNHLYMQRGK GPS+VALGTTHRFL+KYVTVVLY
Sbjct: 241 LQRTLLNSPSTYMEIPTCLSRPQHVVLNHLYMQRGKGGPSVVALGTTHRFLSKYVTVVLY 300

[Close]

BLAST of Carg25121 vs. ExPASy Swiss-Prot
Match: Q9SCY5 (SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX=3702 GN=KINB2 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 8.5e-101
Identity = 187/299 (62.54%), Postives = 232/299 (77.59%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGV-AVADGAHILYRPQGVCQKLLGGQSP 60
           MGNVN RE   E + ++ +  E E+  +   E + A +DG H       V    L GQSP
Sbjct: 1   MGNVNARE---EANSNNASAVEDEDAEICSREAMSAASDGNH-------VAPPELMGQSP 60

Query: 61  PQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETC-EQSIPTMITWSY 120
           P SP AT+SPL+F PQVP+ PL++PDE+ + + S M   S  YEE   EQ IPTMITW +
Sbjct: 61  PHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSWMQSPSSLYEEASNEQGIPTMITWCH 120

Query: 121 GGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDD 180
           GGKE+A+EGSWD W+TR+ LQRSG+DFTIMKVLP+GVY+YRF+VDGQWR++PELP  +DD
Sbjct: 121 GGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDD 180

Query: 181 VGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLL 240
            GN +N+LDLQD VPEDI+SIS FEPPQSP++SY NLLLG++DY+KEPP+VPPHLQ TLL
Sbjct: 181 AGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLL 240

Query: 241 DSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 298
           + P++  +IP  L RPQHV+LNHLYMQ+GKSGPS+VALG+THRFL KYVTVVLYKS QR
Sbjct: 241 NLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 289

[Close]

BLAST of Carg25121 vs. ExPASy Swiss-Prot
Match: Q84VQ1 (SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX=3702 GN=KINB1 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 9.6e-60
Identity = 134/301 (44.52%), Postives = 183/301 (60.80%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60
           MGN NG+   DE+        +V       + G   A   H   RP           SPP
Sbjct: 1   MGNANGK---DEDAAAGSGGADVTSSSARSNGGDPSARSRH--RRPSSDSM----SSSPP 60

Query: 61  QSPTATRSPLIFTPQVPLTPLRK----PDEMLVISQSQMMQGSLGYEETCEQSIPTMITW 120
            SP  + SP +F PQVP+ PL++    P   +  +QSQ +     ++   EQ IPT+ITW
Sbjct: 61  GSPARSPSPFLFAPQVPVAPLQRANAPPPNNIQWNQSQRV-----FDNPPEQGIPTIITW 120

Query: 121 SYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQ 180
           + GG +VA+EGSWD WR+R  LQ+SG+D +I+ VLP+G+Y Y+ +VDG+ +Y P+LP+  
Sbjct: 121 NQGGNDVAVEGSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVA 180

Query: 181 DDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKT 240
           D+VGN  N+LD+ + VPE+ ESI  FE P SPD SY   L  ++DYAKEP  VPP L  T
Sbjct: 181 DEVGNVCNILDVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLT 240

Query: 241 LLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQ 298
           LL +     E     ++PQHVVLNH+++++G +  SIVALG THRF +KY+TVVLYK   
Sbjct: 241 LLGT----TEETAIATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLT 283

[Close]

BLAST of Carg25121 vs. ExPASy Swiss-Prot
Match: O43741 (5'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.6e-30
Identity = 86/255 (33.73%), Postives = 133/255 (52.16%), Query Frame = 0


Query: 55  GGQSPPQSPT----ATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSI 114
           GG +P +       +T  P +F+    L   + P +   +S  Q ++ S+   +  +Q+ 
Sbjct: 25  GGHAPGKEHKIMVGSTDDPSVFS----LPDSKLPGDKEFVSWQQDLEDSV---KPTQQAR 84

Query: 115 PTMITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSP 174
           PT+I WS GGKEV I GS++ W T+ PL +S  DF  +  LP G +QY+F VDGQW + P
Sbjct: 85  PTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDP 144

Query: 175 ELPWGQDDVGNAYNVLDLQDNVPE-----DIESISSFEP-----PQSPDSSYDNLLLG-- 234
             P     +G   N++ ++ +  E      ++S+ S E        SP   Y   +    
Sbjct: 145 SEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFR 204

Query: 235 SDDYAKEPPLVPPHLQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGT 294
           S++  K PP++PPHL + +L+  ++    P  L  P HV+LNHLY    K   S++ L  
Sbjct: 205 SEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKD--SVMVLSA 264

[Close]

BLAST of Carg25121 vs. ExPASy Swiss-Prot
Match: Q9QZH4 (5'-AMP-activated protein kinase subunit beta-2 OS=Rattus norvegicus OX=10116 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.0e-30
Identity = 75/199 (37.69%), Postives = 111/199 (55.78%), Query Frame = 0


Query: 107 EQSIPTMITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQW 166
           +Q+ PT+I WS GGKEV I GS++ W T+ PL +S  DF  +  LP G +QY+F VDGQW
Sbjct: 73  QQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQW 132

Query: 167 RYSPELPWGQDDVGNAYNVLDLQDNVPE-----DIESISSFEP-----PQSPDSSY--DN 226
            + P  P     +G   N++ ++ +  E      ++S+ S E        SP   Y  + 
Sbjct: 133 VHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEM 192

Query: 227 LLLGSDDYAKEPPLVPPHLQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIV 286
            +  S++  K PP++PPHL + +L+  ++    P  L  P HV+LNHLY    K   S++
Sbjct: 193 YVFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSTKD--SVM 252

Query: 287 ALGTTHRFLTKYVTVVLYK 294
            L  THR+  KYVT +LYK
Sbjct: 253 VLSATHRYKKKYVTTLLYK 269

[Close]

BLAST of Carg25121 vs. ExPASy Swiss-Prot
Match: Q6PAM0 (5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.3e-29
Identity = 82/241 (34.02%), Postives = 127/241 (52.70%), Query Frame = 0


Query: 65  ATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGGKEVA 124
           +T  P +F+    L   + P +   +   Q +  S+   +  +Q+ PT+I WS GGKEV 
Sbjct: 38  STDDPSVFS----LPDSKLPGDKEFVPWQQDLDDSV---KPAQQARPTVIRWSEGGKEVF 97

Query: 125 IEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVGNAYN 184
           I GS++ W T+ PL +S  DF  +  LP G +QY+F VDGQW + P  P     +G   N
Sbjct: 98  ISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINN 157

Query: 185 VLDLQDNVPE-----DIESISSFEP-----PQSPDSSY--DNLLLGSDDYAKEPPLVPPH 244
           ++ ++ +  E      ++S+ S E        SP   Y  +  +  S++  K PP++PPH
Sbjct: 158 LIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEERFKSPPILPPH 217

Query: 245 LQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLY 294
           L + +L+  ++    P  L  P HV+LNHLY    K   S++ L  THR+  KYVT +LY
Sbjct: 218 LLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKD--SVMVLSATHRYKKKYVTTLLY 269

[Close]

BLAST of Carg25121 vs. ExPASy TrEMBL
Match: A0A6J1EHM4 (SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita moschata OX=3662 GN=LOC111434171 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 6.9e-170
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60
           MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP
Sbjct: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60

Query: 61  QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120
           QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG
Sbjct: 61  QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120

Query: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180
           KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG
Sbjct: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180

Query: 181 NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240
           NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS
Sbjct: 181 NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240

Query: 241 PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 298
           PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR
Sbjct: 241 PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 297

[Close]

BLAST of Carg25121 vs. ExPASy TrEMBL
Match: A0A6J1KN65 (SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita maxima OX=3661 GN=LOC111495675 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 2.6e-161
Identity = 285/297 (95.96%), Postives = 290/297 (97.64%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60
           MGNVNGREYVDEEDDD+   EEVEEEGLIV+EGVAVADGAHILYRPQGVCQKL+GGQSPP
Sbjct: 1   MGNVNGREYVDEEDDDE--NEEVEEEGLIVNEGVAVADGAHILYRPQGVCQKLMGGQSPP 60

Query: 61  QSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120
           QSPTA RSPLIFTPQVPLTPLRKP+EMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG
Sbjct: 61  QSPTAPRSPLIFTPQVPLTPLRKPEEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGG 120

Query: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180
           KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG
Sbjct: 121 KEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVG 180

Query: 181 NAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240
           NAYNVLDLQDNVPEDI+SIS FEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS
Sbjct: 181 NAYNVLDLQDNVPEDIKSISCFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDS 240

Query: 241 PSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 298
           PSSYME PP LSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR
Sbjct: 241 PSSYMETPPSLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQR 295

[Close]

BLAST of Carg25121 vs. ExPASy TrEMBL
Match: A0A1S3CJK5 (SNF1-related protein kinase regulatory subunit beta-2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501706 PE=3 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.6e-142
Identity = 259/305 (84.92%), Postives = 276/305 (90.49%), Query Frame = 0


Query: 1   MGNVNGREYVDEE-----DDDDENEEEVEEE--GLIVHEGVAVADGAHILYRPQGVCQKL 60
           MGNVNGREYVDE      +++DENEEEVEEE  GLIVHEGV +ADGA IL    GVC KL
Sbjct: 1   MGNVNGREYVDESPSGSVEENDENEEEVEEEEDGLIVHEGVPMADGAPILCHSLGVCSKL 60

Query: 61  LGGQSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETC-EQSIPT 120
           + GQSPPQSPTATRSPLIFTPQVPLTPLRK DEML+ + SQ MQ SLG+E+TC EQSIPT
Sbjct: 61  MRGQSPPQSPTATRSPLIFTPQVPLTPLRKADEMLIHAHSQ-MQSSLGFEDTCNEQSIPT 120

Query: 121 MITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPEL 180
           MITWSYGGKEVAIEGSWD WRTR PLQRSG+DFT+MKVLPAGVYQYRF VDGQWRY+PEL
Sbjct: 121 MITWSYGGKEVAIEGSWDHWRTRMPLQRSGKDFTLMKVLPAGVYQYRFFVDGQWRYAPEL 180

Query: 181 PWGQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPH 240
           PW QDD GNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPH
Sbjct: 181 PWAQDDSGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPH 240

Query: 241 LQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLY 298
           LQ+TLL+SPS+YMEIP CLSRPQHVVLNHLYMQRGK GPS+VALGTTHRFL+KYVTVVLY
Sbjct: 241 LQRTLLNSPSTYMEIPTCLSRPQHVVLNHLYMQRGKGGPSVVALGTTHRFLSKYVTVVLY 300

[Close]

BLAST of Carg25121 vs. ExPASy TrEMBL
Match: A0A0A0LYK2 (AMPKBI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G181340 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.0e-140
Identity = 254/307 (82.74%), Postives = 272/307 (88.60%), Query Frame = 0


Query: 1   MGNVNGREYVDE---------EDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQ 60
           MGNVNGREYVDE         E++++E EEE EE+ LIVHEGV + DGA IL   QGVC 
Sbjct: 1   MGNVNGREYVDESPSGSVEENEEEEEEIEEEEEEDELIVHEGVPMPDGAPILCHSQGVCS 60

Query: 61  KLLGGQSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETC-EQSI 120
           KL+ GQSPPQSPT TRSPLIFTPQVPLTPLRK DEML+ + SQ MQ SL YE+TC EQSI
Sbjct: 61  KLMRGQSPPQSPTTTRSPLIFTPQVPLTPLRKADEMLIHTHSQ-MQSSLAYEDTCNEQSI 120

Query: 121 PTMITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSP 180
           PTMITWSYGGKEV IEGSWD WRTR PLQRSG+DFT+MKVLPAGVYQYRFLVDGQWRY+P
Sbjct: 121 PTMITWSYGGKEVTIEGSWDCWRTRMPLQRSGKDFTLMKVLPAGVYQYRFLVDGQWRYAP 180

Query: 181 ELPWGQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVP 240
           ELPW QDD GNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVP
Sbjct: 181 ELPWAQDDAGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVP 240

Query: 241 PHLQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVV 298
           PHLQ+TLL+SPS+YMEIP CLSRPQHVVLNHLYMQRGK GPS+VALGTTHRFL+KYVTVV
Sbjct: 241 PHLQRTLLNSPSTYMEIPTCLSRPQHVVLNHLYMQRGKGGPSVVALGTTHRFLSKYVTVV 300

[Close]

BLAST of Carg25121 vs. ExPASy TrEMBL
Match: A0A6J1CLZ4 (SNF1-related protein kinase regulatory subunit beta-2 OS=Momordica charantia OX=3673 GN=LOC111012688 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-139
Identity = 251/302 (83.11%), Postives = 269/302 (89.07%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVE----EEGLIVHEGVAVADGAHILYRPQGVCQKLLGG 60
           MGNVNGREY D+     E EEEVE    E+G+IVHEGVAVADGAHI Y PQGVC KL+ G
Sbjct: 1   MGNVNGREYEDDTPSGSEEEEEVEGEVGEDGVIVHEGVAVADGAHIGYHPQGVCSKLVSG 60

Query: 61  QSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQMMQGSLGYEETC-EQSIPTMIT 120
           QSPP SP ATRSPLIFTPQVPLTPLRKPDEML+ S  + M  S GYE TC E S+PTMIT
Sbjct: 61  QSPPHSPAATRSPLIFTPQVPLTPLRKPDEMLIPSDYR-MPNSFGYEGTCNEPSVPTMIT 120

Query: 121 WSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWG 180
           WSYGGKEVA+EGSWDLW+TR PLQRSG+DFTIMKVLPAGVYQYRFL+DGQWRY+PELPW 
Sbjct: 121 WSYGGKEVAVEGSWDLWKTRMPLQRSGKDFTIMKVLPAGVYQYRFLIDGQWRYAPELPWA 180

Query: 181 QDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQK 240
           QD+ GNAYNVLDLQDNVPEDIESISSFEPPQSPDSSY+N+LLGSDDYAKEPPLVPPHLQ+
Sbjct: 181 QDNAGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYNNVLLGSDDYAKEPPLVPPHLQR 240

Query: 241 TLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSF 298
           TLL+SPSSYMEIP  LSRPQHV+LNHLYMQRGKSGPSIVALGTTHRFL KYVTVVLYKSF
Sbjct: 241 TLLNSPSSYMEIPHDLSRPQHVILNHLYMQRGKSGPSIVALGTTHRFLAKYVTVVLYKSF 300

[Close]

BLAST of Carg25121 vs. TAIR 10
Match: AT4G16360.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 366.3 bits (939), Expect = 2.3e-101
Identity = 176/264 (66.67%), Postives = 214/264 (81.06%), Query Frame = 0


Query: 35  AVADGAHILYRPQGVCQKLLGGQSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQ 94
           A +DG H       V    L GQSPP SP AT+SPL+F PQVP+ PL++PDE+ + + S 
Sbjct: 3   AASDGNH-------VAPPELMGQSPPHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSW 62

Query: 95  MMQGSLGYEETC-EQSIPTMITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPA 154
           M   S  YEE   EQ IPTMITW +GGKE+A+EGSWD W+TR+ LQRSG+DFTIMKVLP+
Sbjct: 63  MQSPSSLYEEASNEQGIPTMITWCHGGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPS 122

Query: 155 GVYQYRFLVDGQWRYSPELPWGQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYD 214
           GVY+YRF+VDGQWR++PELP  +DD GN +N+LDLQD VPEDI+SIS FEPPQSP++SY 
Sbjct: 123 GVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYS 182

Query: 215 NLLLGSDDYAKEPPLVPPHLQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSI 274
           NLLLG++DY+KEPP+VPPHLQ TLL+ P++  +IP  L RPQHV+LNHLYMQ+GKSGPS+
Sbjct: 183 NLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSV 242

Query: 275 VALGTTHRFLTKYVTVVLYKSFQR 298
           VALG+THRFL KYVTVVLYKS QR
Sbjct: 243 VALGSTHRFLAKYVTVVLYKSLQR 259

[Close]

BLAST of Carg25121 vs. TAIR 10
Match: AT4G16360.3 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 366.3 bits (939), Expect = 2.3e-101
Identity = 176/264 (66.67%), Postives = 214/264 (81.06%), Query Frame = 0


Query: 35  AVADGAHILYRPQGVCQKLLGGQSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQ 94
           A +DG H       V    L GQSPP SP AT+SPL+F PQVP+ PL++PDE+ + + S 
Sbjct: 5   AASDGNH-------VAPPELMGQSPPHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSW 64

Query: 95  MMQGSLGYEETC-EQSIPTMITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPA 154
           M   S  YEE   EQ IPTMITW +GGKE+A+EGSWD W+TR+ LQRSG+DFTIMKVLP+
Sbjct: 65  MQSPSSLYEEASNEQGIPTMITWCHGGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPS 124

Query: 155 GVYQYRFLVDGQWRYSPELPWGQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYD 214
           GVY+YRF+VDGQWR++PELP  +DD GN +N+LDLQD VPEDI+SIS FEPPQSP++SY 
Sbjct: 125 GVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYS 184

Query: 215 NLLLGSDDYAKEPPLVPPHLQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSI 274
           NLLLG++DY+KEPP+VPPHLQ TLL+ P++  +IP  L RPQHV+LNHLYMQ+GKSGPS+
Sbjct: 185 NLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSV 244

Query: 275 VALGTTHRFLTKYVTVVLYKSFQR 298
           VALG+THRFL KYVTVVLYKS QR
Sbjct: 245 VALGSTHRFLAKYVTVVLYKSLQR 261

[Close]

BLAST of Carg25121 vs. TAIR 10
Match: AT4G16360.2 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.2e-99
Identity = 175/264 (66.29%), Postives = 213/264 (80.68%), Query Frame = 0


Query: 35  AVADGAHILYRPQGVCQKLLGGQSPPQSPTATRSPLIFTPQVPLTPLRKPDEMLVISQSQ 94
           A +DG H       V    L GQSPP SP AT+SPL+F PQVP+ PL++PDE+ + + S 
Sbjct: 3   AASDGNH-------VAPPELMGQSPPHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSW 62

Query: 95  MMQGSLGYEETC-EQSIPTMITWSYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPA 154
           M   S  YEE   EQ IPTMITW +GGKE+A+EGSWD W+T + LQRSG+DFTIMKVLP+
Sbjct: 63  MQSPSSLYEEASNEQGIPTMITWCHGGKEIAVEGSWDNWKT-SRLQRSGKDFTIMKVLPS 122

Query: 155 GVYQYRFLVDGQWRYSPELPWGQDDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYD 214
           GVY+YRF+VDGQWR++PELP  +DD GN +N+LDLQD VPEDI+SIS FEPPQSP++SY 
Sbjct: 123 GVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYS 182

Query: 215 NLLLGSDDYAKEPPLVPPHLQKTLLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSI 274
           NLLLG++DY+KEPP+VPPHLQ TLL+ P++  +IP  L RPQHV+LNHLYMQ+GKSGPS+
Sbjct: 183 NLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSV 242

Query: 275 VALGTTHRFLTKYVTVVLYKSFQR 298
           VALG+THRFL KYVTVVLYKS QR
Sbjct: 243 VALGSTHRFLAKYVTVVLYKSLQR 258

[Close]

BLAST of Carg25121 vs. TAIR 10
Match: AT5G21170.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 231.9 bits (590), Expect = 6.8e-61
Identity = 134/301 (44.52%), Postives = 183/301 (60.80%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60
           MGN NG+   DE+        +V       + G   A   H   RP           SPP
Sbjct: 1   MGNANGK---DEDAAAGSGGADVTSSSARSNGGDPSARSRH--RRPSSDSM----SSSPP 60

Query: 61  QSPTATRSPLIFTPQVPLTPLRK----PDEMLVISQSQMMQGSLGYEETCEQSIPTMITW 120
            SP  + SP +F PQVP+ PL++    P   +  +QSQ +     ++   EQ IPT+ITW
Sbjct: 61  GSPARSPSPFLFAPQVPVAPLQRANAPPPNNIQWNQSQRV-----FDNPPEQGIPTIITW 120

Query: 121 SYGGKEVAIEGSWDLWRTRTPLQRSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQ 180
           + GG +VA+EGSWD WR+R  LQ+SG+D +I+ VLP+G+Y Y+ +VDG+ +Y P+LP+  
Sbjct: 121 NQGGNDVAVEGSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVA 180

Query: 181 DDVGNAYNVLDLQDNVPEDIESISSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKT 240
           D+VGN  N+LD+ + VPE+ ESI  FE P SPD SY   L  ++DYAKEP  VPP L  T
Sbjct: 181 DEVGNVCNILDVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLT 240

Query: 241 LLDSPSSYMEIPPCLSRPQHVVLNHLYMQRGKSGPSIVALGTTHRFLTKYVTVVLYKSFQ 298
           LL +     E     ++PQHVVLNH+++++G +  SIVALG THRF +KY+TVVLYK   
Sbjct: 241 LLGT----TEETAIATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLT 283

[Close]

BLAST of Carg25121 vs. TAIR 10
Match: AT5G21170.2 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 213.4 bits (542), Expect = 2.5e-55
Identity = 134/338 (39.64%), Postives = 183/338 (54.14%), Query Frame = 0


Query: 1   MGNVNGREYVDEEDDDDENEEEVEEEGLIVHEGVAVADGAHILYRPQGVCQKLLGGQSPP 60
           MGN NG+   DE+        +V       + G   A   H   RP           SPP
Sbjct: 1   MGNANGK---DEDAAAGSGGADVTSSSARSNGGDPSARSRH--RRPSSDSM----SSSPP 60

Query: 61  QSPTATRSPLIFTPQ-------------------------------------VPLTPLRK 120
            SP  + SP +F PQ                                     VP+ PL++
Sbjct: 61  GSPARSPSPFLFAPQPTLLFHVTSYNICSPMRHLFHCLGLTCVPNGCISCRMVPVAPLQR 120

Query: 121 ----PDEMLVISQSQMMQGSLGYEETCEQSIPTMITWSYGGKEVAIEGSWDLWRTRTPLQ 180
               P   +  +QSQ +     ++   EQ IPT+ITW+ GG +VA+EGSWD WR+R  LQ
Sbjct: 121 ANAPPPNNIQWNQSQRV-----FDNPPEQGIPTIITWNQGGNDVAVEGSWDNWRSRKKLQ 180

Query: 181 RSGRDFTIMKVLPAGVYQYRFLVDGQWRYSPELPWGQDDVGNAYNVLDLQDNVPEDIESI 240
           +SG+D +I+ VLP+G+Y Y+ +VDG+ +Y P+LP+  D+VGN  N+LD+ + VPE+ ESI
Sbjct: 181 KSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADEVGNVCNILDVHNFVPENPESI 240

Query: 241 SSFEPPQSPDSSYDNLLLGSDDYAKEPPLVPPHLQKTLLDSPSSYMEIPPCLSRPQHVVL 298
             FE P SPD SY   L  ++DYAKEP  VPP L  TLL +     E     ++PQHVVL
Sbjct: 241 VEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLLGT----TEETAIATKPQHVVL 300

The following BLAST results are available for this feature:

BLAST of Carg25121 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927294.1	1.4e-169	100.00	SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita moschata] ...	[more]
XP_023520032.1	2.3e-167	99.33	SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita pepo subsp...	[more]
XP_023001594.1	5.4e-161	95.96	SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita maxima]	[more]
XP_038895464.1	3.3e-142	84.92	SNF1-related protein kinase regulatory subunit beta-2-like [Benincasa hispida]	[more]
XP_008463594.1	7.4e-142	84.92	PREDICTED: SNF1-related protein kinase regulatory subunit beta-2 isoform X2 [Cuc...	[more]

BLAST of Carg25121 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCY5	8.5e-101	62.54	SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX...	[more]
Q84VQ1	9.6e-60	44.52	SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX...	[more]
O43741	4.6e-30	33.73	5'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2...	[more]
Q9QZH4	6.0e-30	37.69	5'-AMP-activated protein kinase subunit beta-2 OS=Rattus norvegicus OX=10116 GN=...	[more]
Q6PAM0	1.3e-29	34.02	5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab...	[more]

BLAST of Carg25121 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EHM4	6.9e-170	100.00	SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita moschata...	[more]
A0A6J1KN65	2.6e-161	95.96	SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita maxima O...	[more]
A0A1S3CJK5	3.6e-142	84.92	SNF1-related protein kinase regulatory subunit beta-2 isoform X2 OS=Cucumis melo...	[more]
A0A0A0LYK2	2.0e-140	82.74	AMPKBI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G181340 PE=3...	[more]
A0A6J1CLZ4	1.3e-139	83.11	SNF1-related protein kinase regulatory subunit beta-2 OS=Momordica charantia OX=...	[more]

BLAST of Carg25121 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16360.1	2.3e-101	66.67	5'-AMP-activated protein kinase beta-2 subunit protein	[more]
AT4G16360.3	2.3e-101	66.67	5'-AMP-activated protein kinase beta-2 subunit protein	[more]
AT4G16360.2	2.2e-99	66.29	5'-AMP-activated protein kinase beta-2 subunit protein	[more]
AT5G21170.1	6.8e-61	44.52	5'-AMP-activated protein kinase beta-2 subunit protein	[more]
AT5G21170.2	2.5e-55	39.64	5'-AMP-activated protein kinase beta-2 subunit protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006828	Association with the SNF1 complex (ASC) domain	SMART	SM01010	AMPKBI_2	coord: 205..295 e-value: 5.4E-29 score: 112.3
IPR006828	Association with the SNF1 complex (ASC) domain	PFAM	PF04739	AMPKBI	coord: 223..293 e-value: 1.1E-20 score: 73.6
IPR032640	AMP-activated protein kinase, glycogen-binding domain	PFAM	PF16561	AMPK1_CBM	coord: 110..190 e-value: 1.1E-29 score: 102.4
IPR013783	Immunoglobulin-like fold	GENE3D	2.60.40.10	Immunoglobulins	coord: 102..193 e-value: 3.5E-23 score: 83.2
None	No IPR available	GENE3D	6.20.250.60		coord: 198..295 e-value: 3.0E-24 score: 87.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 9..26
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR46316:SF2	SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-2	coord: 1..297
None	No IPR available	CDD	cd02859	E_set_AMPKbeta_like_N	coord: 111..187 e-value: 2.22041E-25 score: 94.588
IPR043554	SNF1-related protein kinase regulatory subunit beta	PANTHER	PTHR46316	SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1	coord: 1..297
IPR014756	Immunoglobulin E-set	SUPERFAMILY	81296	E set domains	coord: 110..195
IPR037256	ASC domain superfamily	SUPERFAMILY	160219	AMPKBI-like	coord: 192..294

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25121-RA	Carg25121-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005515	protein binding