Carg25041 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25041
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionIsoaspartyl peptidase/L-asparaginase
LocationCarg_Chr11: 6122098 .. 6123809 (+)
RNA-Seq ExpressionCarg25041
SyntenyCarg25041
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACCGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGTTAGAATTTCTACCCCGTTCCTATGTACACAGATAGATGCTTTGCGATTCATGACTGTTTTATTCGTGCACCAATTTCTTACTGTTCAATTTTCTTAAAATTGTCAAGGGAGTGGAGACCACTGATCCAAGCTACTTTATCACCCCACATAACATTGAAAGGCTAAAACAAGCTAAAGAGGCCAACAGTGTGCAGGTTTGCTTCTTATCTTTGTTGAGGACTGTTGGGAAATAGTCCCACGTTAGCTAATTTAGGGAATGATCATGGATTTATAAGTAAGGAATACATCTTCATTGGTATGAGGCCTTTTGAGAAAGCTCAAAGCAAAGCCGTTAGAGCTTATACTCAAAGGGGACAATATCATACCATTATGAAGAGCTGTTATTTCTAATGTGGTATCAGAGCCATGCCCTTAATTTTGCCATGACAATAGAATTCTCAAATGTCGAACAAAGAAGTTGTGAGCCTCAAAGGTGTAGTCAAAAGTGACTCATGTGTCAAACAAAGGGTTTACTTTGTTCGAGGGTTCCAGAGAAAAGGAGTCAACCTCGATTAAGGGGAGGCTATTCGAGTTCTGTATAGAACTCAGATGCTCTATGGTGTACTTTGTTCGAGAGGAGGATTGTTGAAGATTGTTAGGAGAGAGTCCCACAATGGCTAATTTAGGGAATGATCATGGGTTTATAAGTAAGAAATACCTCTCCATCGATATGAGGCCTTTTGGGGAAGCCCAAAGAAAAGCCATTAGAGCATATACTCGAAGTGGACAATTTCACACCGTTGTGGAGAGTCGTTATTCCTAACAATCTTCTATCCCAAACATGACTTTGACTGCTCTCTTTATGAACCAAAAGTTATAGGTCTAGATATCCGTCTAATAGGGTTGAAAAAATCACATTATGGAGTTTCCATGAGATTTTTATGTTTTAGCATCGGTTGATTTCTATGGTACTTTTTGTTTCTGCACATTGATAGCTGTTGTCATGAACACTTATAGATTGATTATACCCGACCAAGTCCCCAAGCCACAGTACAAGAAACTGGGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGACACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAATTCAAGGGTCTATCTCTTGAAGAAGCAGCAGCCTATGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCAGCCTCGGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCCAACATGACTGAATGA

mRNA sequence

GTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACCGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGGAGTGGAGACCACTGATCCAAGCTACTTTATCACCCCACATAACATTGAAAGGCTAAAACAAGCTAAAGAGGCCAACAGTGTGCAGATTGATTATACCCGACCAAGTCCCCAAGCCACAGTACAAGAAACTGGGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGACACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAATTCAAGGGTCTATCTCTTGAAGAAGCAGCAGCCTATGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCAGCCTCGGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCCAACATGACTGAATGA

Coding sequence (CDS)

GTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACCGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGGAGTGGAGACCACTGATCCAAGCTACTTTATCACCCCACATAACATTGAAAGGCTAAAACAAGCTAAAGAGGCCAACAGTGTGCAGATTGATTATACCCGACCAAGTCCCCAAGCCACAGTACAAGAAACTGGGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGACACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAATTCAAGGGTCTATCTCTTGAAGAAGCAGCAGCCTATGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCAGCCTCGGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCCAACATGACTGAATGA

Protein sequence

VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAIWPSNMTE
Homology
[Close]
BLAST of Carg25041 vs. NCBI nr
Match: KAG7022301.1 (Isoaspartyl peptidase/L-asparaginase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 524.6 bits (1350), Expect = 4.9e-145
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT
Sbjct: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE
Sbjct: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS
Sbjct: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN
Sbjct: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240

Query: 241 TTGMFRACATEDGYSEIAIWPSNMTE 267
           TTGMFRACATEDGYSEIAIWPSNMTE
Sbjct: 241 TTGMFRACATEDGYSEIAIWPSNMTE 266

[Close]
BLAST of Carg25041 vs. NCBI nr
Match: KAG6588471.1 (Isoaspartyl peptidase/L-asparaginase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 524.6 bits (1350), Expect = 4.9e-145
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT
Sbjct: 55  VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE
Sbjct: 115 PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS
Sbjct: 175 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN
Sbjct: 235 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPSNMTE 267
           TTGMFRACATEDGYSEIAIWPSNMTE
Sbjct: 295 TTGMFRACATEDGYSEIAIWPSNMTE 320

[Close]
BLAST of Carg25041 vs. NCBI nr
Match: XP_023530268.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514.2 bits (1323), Expect = 6.6e-142
Identity = 262/266 (98.50%), Postives = 263/266 (98.87%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT
Sbjct: 55  VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAEAFAREQGVETTDPSYFITP NIERLKQAKEANSVQIDYT+PSPQAT QE
Sbjct: 115 PHIYLAFDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATGQE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           T IGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS
Sbjct: 175 TEIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN
Sbjct: 235 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPSNMTE 267
           TTGMFRACATEDGYSEIAIWPSNMTE
Sbjct: 295 TTGMFRACATEDGYSEIAIWPSNMTE 320

[Close]
BLAST of Carg25041 vs. NCBI nr
Match: XP_022969815.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita maxima])

HSP 1 Score: 511.5 bits (1316), Expect = 4.3e-141
Identity = 261/266 (98.12%), Postives = 262/266 (98.50%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT
Sbjct: 55  VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAEAFAREQGVETTDPSYFITP NIERLKQAKEANSVQIDYT+PSPQATVQE
Sbjct: 115 PHIYLAFDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS
Sbjct: 175 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIRGTVARDVAALMEFK LSLE AAA AVESVPKGNVGLIAVSASGEVAMPFN
Sbjct: 235 ATGKGEAIIRGTVARDVAALMEFKDLSLEAAAACAVESVPKGNVGLIAVSASGEVAMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPSNMTE 267
           TTGMFRACATEDGYSEIAIWPSNMTE
Sbjct: 295 TTGMFRACATEDGYSEIAIWPSNMTE 320

[Close]
BLAST of Carg25041 vs. NCBI nr
Match: XP_022933679.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita moschata])

HSP 1 Score: 509.6 bits (1311), Expect = 1.6e-140
Identity = 260/266 (97.74%), Postives = 262/266 (98.50%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT
Sbjct: 55  VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAEAFAREQGVETTDPSYFITP NIERLKQAKEANSVQIDYT+PSPQATVQE
Sbjct: 115 PHIYLAFDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           T IGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS
Sbjct: 175 TEIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIRGTVARDVAALMEFK LSLEEAAA AVESVPKGNVGLIAVSASG+VAMPFN
Sbjct: 235 ATGKGEAIIRGTVARDVAALMEFKDLSLEEAAACAVESVPKGNVGLIAVSASGDVAMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPSNMTE 267
           TTGMFRACATEDGYSEIAIWPSNMTE
Sbjct: 295 TTGMFRACATEDGYSEIAIWPSNMTE 320

[Close]
BLAST of Carg25041 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 407.9 bits (1047), Expect = 8.7e-113
Identity = 215/265 (81.13%), Postives = 231/265 (87.17%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEK 60
           VRELEN P FNAG+GSVLT  GTVEMEASIMDGK KRCGAVSGLTTVVN ISLARLVMEK
Sbjct: 56  VRELENHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEK 115

Query: 61  TPHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQ 120
           TPHIYLAFD AEAFAR  GVET D S+FITP NI RLKQAKE N VQ+DYT PSP+    
Sbjct: 116 TPHIYLAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVP-- 175

Query: 121 ETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAV 180
                 GDSQIGTVGCVAVD+ GNLA+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+
Sbjct: 176 ---DNCGDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAI 235

Query: 181 SATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMP 240
           SATGKGE IIRGTVARDVAALME+KGLSL EAAAY V +SVP+G+ GL+AVSA+GEV MP
Sbjct: 236 SATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMP 295

Query: 241 FNTTGMFRACATEDGYSEIAIWPSN 264
           FNTTGMFRACA+EDGYSEIAIWP+N
Sbjct: 296 FNTTGMFRACASEDGYSEIAIWPNN 315

[Close]
BLAST of Carg25041 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 1.7e-108
Identity = 208/269 (77.32%), Postives = 230/269 (85.50%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEK 60
           VRELEN  HFNAG GSVLT +GTVEMEASIMDGK  +CGAVSGL+TV+N ISLARLVM+K
Sbjct: 56  VRELENIQHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDK 115

Query: 61  TPHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYT-----RPSP 120
           TPHIYLAF GA+ FA++QGVET D S+FIT  N+ERLK A EAN VQ+DY+     +P+ 
Sbjct: 116 TPHIYLAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQ 175

Query: 121 QATVQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYAN 180
               +E  + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN
Sbjct: 176 DDAEKELPLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 235

Query: 181 HLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASG 240
            LCAVSATGKGEAII  TVARDVAALMEFKGLSL+EAA Y V E  PKG VGLIAVSA+G
Sbjct: 236 ELCAVSATGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAG 295

Query: 241 EVAMPFNTTGMFRACATEDGYSEIAIWPS 263
           E+AMPFNTTGMFRA ATEDGYSEIAIWP+
Sbjct: 296 EIAMPFNTTGMFRASATEDGYSEIAIWPT 324

[Close]
BLAST of Carg25041 vs. ExPASy Swiss-Prot
Match: P30362 (Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 5.0e-108
Identity = 209/268 (77.99%), Postives = 228/268 (85.07%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKK-RCGAVSGLTTVVNAISLARLVMEK 60
           VRELEN  HFNAG GSVLT +GTVEMEASIMDGK  +CGAVSGL+TV+N ISLARLVMEK
Sbjct: 37  VRELENNEHFNAGIGSVLTNSGTVEMEASIMDGKSMKCGAVSGLSTVLNPISLARLVMEK 96

Query: 61  TPHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQID-----YTRPSP 120
           TPH+YLAF GA+ FA++QGVET D S+FIT  N+ERLK A EAN VQID     YT+P  
Sbjct: 97  TPHMYLAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQIDYSQYNYTQPVQ 156

Query: 121 QATVQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYAN 180
               +E  + NGDSQIGTVGCVAVD+ GNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN
Sbjct: 157 DDAEKELPVANGDSQIGTVGCVAVDSQGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 216

Query: 181 HLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASG 240
            LCAVSATGKGEAII+ TVARDVAALMEFKGLSL+EAA Y V E  PKG VGLIAVSA+G
Sbjct: 217 ELCAVSATGKGEAIIQATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAG 276

Query: 241 EVAMPFNTTGMFRACATEDGYSEIAIWP 262
           E+AMPFNTTGMFRACATEDG SEIAIWP
Sbjct: 277 EIAMPFNTTGMFRACATEDGNSEIAIWP 304

[Close]
BLAST of Carg25041 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 2.5e-107
Identity = 205/269 (76.21%), Postives = 228/269 (84.76%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEK 60
           VRELEN  HFNAG GSVLT +GTVEMEASIMDG   +CGAVSGL+TV+N ISLARLVM+K
Sbjct: 56  VRELENIEHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDK 115

Query: 61  TPHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTR-----PSP 120
           TPHIYLAF GA+ FA++QGVET D S+ IT  N+ERLK A EAN VQ+DY++     P  
Sbjct: 116 TPHIYLAFQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVK 175

Query: 121 QATVQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYAN 180
               +E  + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN
Sbjct: 176 DDAEKELPLTNGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 235

Query: 181 HLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASG 240
            LCAVSATGKGE IIR TVARDVAALMEFKGLSL+EAA + + E  PKG VGLIAVSA+G
Sbjct: 236 ELCAVSATGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAG 295

Query: 241 EVAMPFNTTGMFRACATEDGYSEIAIWPS 263
           E+AMPFNTTGMFRACATEDGYSEIAIWP+
Sbjct: 296 EIAMPFNTTGMFRACATEDGYSEIAIWPT 324

[Close]
BLAST of Carg25041 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 2.1e-106
Identity = 205/269 (76.21%), Postives = 225/269 (83.64%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEK 60
           VRELEN  HFNAG GSVLT +GTVEMEASIMDG   +CGAVSGL TV+N ISLAR VM+K
Sbjct: 56  VRELENIQHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDK 115

Query: 61  TPHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQID-----YTRPSP 120
           TPHI+LAF GA+   ++QGVET D S+FIT  N+ERLK A EAN VQ+D     YT+P  
Sbjct: 116 TPHIFLAFQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQ 175

Query: 121 QATVQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYAN 180
               +E  + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN
Sbjct: 176 DDAEKELPLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 235

Query: 181 HLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASG 240
            LCAVSATGKGEAIIR TVARDVAALMEFKGLSL+EAA   V E  PKG VGLIAVSA+G
Sbjct: 236 ELCAVSATGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAG 295

Query: 241 EVAMPFNTTGMFRACATEDGYSEIAIWPS 263
           E+AMPFNTTGMFRACATEDGYSEIAIWP+
Sbjct: 296 EIAMPFNTTGMFRACATEDGYSEIAIWPT 324

[Close]
BLAST of Carg25041 vs. ExPASy TrEMBL
Match: A0A6J1I105 (isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita maxima OX=3661 GN=LOC111468901 PE=4 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.1e-141
Identity = 261/266 (98.12%), Postives = 262/266 (98.50%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT
Sbjct: 55  VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAEAFAREQGVETTDPSYFITP NIERLKQAKEANSVQIDYT+PSPQATVQE
Sbjct: 115 PHIYLAFDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS
Sbjct: 175 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIRGTVARDVAALMEFK LSLE AAA AVESVPKGNVGLIAVSASGEVAMPFN
Sbjct: 235 ATGKGEAIIRGTVARDVAALMEFKDLSLEAAAACAVESVPKGNVGLIAVSASGEVAMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPSNMTE 267
           TTGMFRACATEDGYSEIAIWPSNMTE
Sbjct: 295 TTGMFRACATEDGYSEIAIWPSNMTE 320

[Close]
BLAST of Carg25041 vs. ExPASy TrEMBL
Match: A0A6J1F0F1 (isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita moschata OX=3662 GN=LOC111441019 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 7.9e-141
Identity = 260/266 (97.74%), Postives = 262/266 (98.50%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT
Sbjct: 55  VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAEAFAREQGVETTDPSYFITP NIERLKQAKEANSVQIDYT+PSPQATVQE
Sbjct: 115 PHIYLAFDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           T IGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS
Sbjct: 175 TEIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIRGTVARDVAALMEFK LSLEEAAA AVESVPKGNVGLIAVSASG+VAMPFN
Sbjct: 235 ATGKGEAIIRGTVARDVAALMEFKDLSLEEAAACAVESVPKGNVGLIAVSASGDVAMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPSNMTE 267
           TTGMFRACATEDGYSEIAIWPSNMTE
Sbjct: 295 TTGMFRACATEDGYSEIAIWPSNMTE 320

[Close]
BLAST of Carg25041 vs. ExPASy TrEMBL
Match: A0A6J1DSP7 (isoaspartyl peptidase/L-asparaginase 1 OS=Momordica charantia OX=3673 GN=LOC111023560 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 6.9e-129
Identity = 239/262 (91.22%), Postives = 249/262 (95.04%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELEN PHFNAGRGSVLTTNGTVEMEASIMD  KRCGAVSGLTTVVNAISLARLVMEKT
Sbjct: 55  VRELENNPHFNAGRGSVLTTNGTVEMEASIMDSTKRCGAVSGLTTVVNAISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYL FDGAEAFAREQGVETTD S+FITP NIERLKQAKEAN VQIDYT+P PQA  +E
Sbjct: 115 PHIYLGFDGAEAFAREQGVETTDSSHFITPENIERLKQAKEANRVQIDYTQPIPQAPAEE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           T I NGDSQ+GTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTP+IGAGTYAN+LCAVS
Sbjct: 175 TEIPNGDSQLGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPVIGAGTYANNLCAVS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIRGTVARDVAALMEFKGLSL+EAAAYAVE+VP+GNVGL+AVSASGEV MPFN
Sbjct: 235 ATGKGEAIIRGTVARDVAALMEFKGLSLKEAAAYAVETVPRGNVGLVAVSASGEVTMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPS 263
           TTGMFRACATEDGYSEIAIWPS
Sbjct: 295 TTGMFRACATEDGYSEIAIWPS 316

[Close]
BLAST of Carg25041 vs. ExPASy TrEMBL
Match: A0A5A7UBC6 (Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00060 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.7e-127
Identity = 238/262 (90.84%), Postives = 244/262 (93.13%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTT GTVEMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKT
Sbjct: 55  VRELENCPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAE FAREQGVET D SYFITP NIERL+QAKEAN VQIDYT+P PQAT QE
Sbjct: 115 PHIYLAFDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           T   NGDSQIGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTP+IGAGTYANHLCA+S
Sbjct: 175 TENSNGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAIS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIR TVARDVAALMEFKGLSLEEAAA  VESVPKGNVGLIAVSASGEVAMPFN
Sbjct: 235 ATGKGEAIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPS 263
           TTGMFRACATEDGYSEI+IW S
Sbjct: 295 TTGMFRACATEDGYSEISIWSS 316

[Close]
BLAST of Carg25041 vs. ExPASy TrEMBL
Match: A0A5D3CEE4 (Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003800 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 6.5e-127
Identity = 237/262 (90.46%), Postives = 243/262 (92.75%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKT 60
           VRELENCPHFNAGRGSVLTT GTVEMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKT
Sbjct: 55  VRELENCPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKT 114

Query: 61  PHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQE 120
           PHIYLAFDGAE FAREQGVET D SYFITP NIERL+QAKEAN VQIDYT+P PQAT QE
Sbjct: 115 PHIYLAFDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQE 174

Query: 121 TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVS 180
           T   NGDS IGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTP+IGAGTYANHLCA+S
Sbjct: 175 TENSNGDSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAIS 234

Query: 181 ATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFN 240
           ATGKGEAIIR TVARDVAALMEFKGLSLEEAAA  VESVPKGNVGLIAVSASGEVAMPFN
Sbjct: 235 ATGKGEAIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFN 294

Query: 241 TTGMFRACATEDGYSEIAIWPS 263
           TTGMFRACATEDGYSEI+IW S
Sbjct: 295 TTGMFRACATEDGYSEISIWSS 316

[Close]
BLAST of Carg25041 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 407.9 bits (1047), Expect = 6.2e-114
Identity = 215/265 (81.13%), Postives = 231/265 (87.17%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEK 60
           VRELEN P FNAG+GSVLT  GTVEMEASIMDGK KRCGAVSGLTTVVN ISLARLVMEK
Sbjct: 56  VRELENHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEK 115

Query: 61  TPHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQ 120
           TPHIYLAFD AEAFAR  GVET D S+FITP NI RLKQAKE N VQ+DYT PSP+    
Sbjct: 116 TPHIYLAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVP-- 175

Query: 121 ETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAV 180
                 GDSQIGTVGCVAVD+ GNLA+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+
Sbjct: 176 ---DNCGDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAI 235

Query: 181 SATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMP 240
           SATGKGE IIRGTVARDVAALME+KGLSL EAAAY V +SVP+G+ GL+AVSA+GEV MP
Sbjct: 236 SATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMP 295

Query: 241 FNTTGMFRACATEDGYSEIAIWPSN 264
           FNTTGMFRACA+EDGYSEIAIWP+N
Sbjct: 296 FNTTGMFRACASEDGYSEIAIWPNN 315

[Close]
BLAST of Carg25041 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 4.2e-102
Identity = 195/240 (81.25%), Postives = 210/240 (87.50%), Query Frame = 0 

Query: 26  MEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAEAFAREQGVETTDP 85
           MEASIMDGK KRCGAVSGLTTVVN ISLARLVMEKTPHIYLAFD AEAFAR  GVET D 
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 86  SYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQETGIGNGDSQIGTVGCVAVDNHGNL 145
           S+FITP NI RLKQAKE N VQ+DYT PSP+          GDSQIGTVGCVAVD+ GNL
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSPKVP-----DNCGDSQIGTVGCVAVDSAGNL 120

Query: 146 AAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAALMEFK 205
           A+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+SATGKGE IIRGTVARDVAALME+K
Sbjct: 121 ASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVAALMEYK 180

Query: 206 GLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAIWPSN 264
           GLSL EAAAY V +SVP+G+ GL+AVSA+GEV MPFNTTGMFRACA+EDGYSEIAIWP+N
Sbjct: 181 GLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEIAIWPNN 235

[Close]
BLAST of Carg25041 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 2.3e-84
Identity = 165/270 (61.11%), Postives = 198/270 (73.33%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDG-KKRCGAVSGLTTVVNAISLARLVMEK 60
           +RELE  P FN+GRGS LT  GTVEMEASIMDG K+RCGAVSG+TTV N ISLARLVM+K
Sbjct: 55  IRELETDPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDK 114

Query: 61  TPHIYLAFDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRP------- 120
           +PH YLAF GAE FAR+QGVE  D  YF+T  N+  LK AKEANS+  DY  P       
Sbjct: 115 SPHSYLAFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGA 174

Query: 121 -SPQATVQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGT 180
            +  + +Q  G+        TVGCV VD  G+ AA TSTGGL+NKM+GRIGD+PLIGAGT
Sbjct: 175 AATDSPIQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGT 234

Query: 181 YANHLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVE-SVPKGNVGLIAVS 240
           YA+  C VS TG+GEAIIR T+ARDV+A+ME+KGL+L+EA  Y ++  + +G  GLIAVS
Sbjct: 235 YASEFCGVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVS 294

Query: 241 ASGEVAMPFNTTGMFRACATEDGYSEIAIW 261
             GEV   FN+ GMFR CATEDG+ E+AIW
Sbjct: 295 NKGEVVCGFNSNGMFRGCATEDGFMEVAIW 324

[Close]
BLAST of Carg25041 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-16
Identity = 76/285 (26.67%), Postives = 122/285 (42.81%), Query Frame = 0 

Query: 1   VRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKK-RCGAVSGLTTVVNAISLARLVME- 60
           ++ LE+ P  NAGRGS LT +G VE +AS+MDG     G V  +  V NAI +A L+++ 
Sbjct: 62  IQVLEDDPSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKE 121

Query: 61  ---------KTPHIYLAFDGAEAFAREQ-----GVETTDPSYFITPHNIERLKQAK---- 120
                    + P + L  +GA  + + +     G  T    + +T     + ++ K    
Sbjct: 122 QITGSTLLGRIPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLS 181

Query: 121 EANSVQIDYTRPSPQAT---------VQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTG 180
           E  +  I      P+ T         V      + D  + TVG + VDN G++A  +S+G
Sbjct: 182 EVGAKSILSAEEHPRGTENNETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSG 241

Query: 181 GLVNKMVGRIGDTPLIGAGTYAN--------HLCAVSATGKGEAIIRGTVARDVAALMEF 239
           G+  K+ GR+G     G+G +A+         L     +G GE ++RG  AR+    +  
Sbjct: 242 GIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLAL 301

[Close]
BLAST of Carg25041 vs. TAIR 10
Match: AT5G61540.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 9.5e-14
Identity = 68/241 (28.22%), Postives = 106/241 (43.98%), Query Frame = 0 

Query: 13  GRGSVLTTNGTVEMEASIMDG-KKRCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAE 72
           G G     NG   ++A +MDG     GAV+ +  V + I  A LVM+ + H  LA +GA 
Sbjct: 80  GPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGAS 139

Query: 73  AFAREQGVETTDPSYFITPHNIERLKQAKE-------------------------ANSVQ 132
           AFA   G+    P    +P ++++    KE                         A +V 
Sbjct: 140 AFAISMGL--PGPMNLSSPESVKKWSDWKENQCQPNFRKNVVPANDCGPYKPNNSAMNVF 199

Query: 133 IDYTRPSPQATVQE-----TGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRI 192
           +D +  S +    E      G  N D    T+    +D  G++A  TST G   K+ GR+
Sbjct: 200 VDKSTESCEMGAIEYKPPLVGPHNHD----TISMAVIDRMGHIAVGTSTNGATYKIPGRV 259

Query: 193 GDTPLIGAGTYA-NHLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVP 222
           GD P++G+  YA + +    ATG G+ ++R      V   M  +G+  EEAA  A+  + 
Sbjct: 260 GDGPIVGSSAYADDEVGGCGATGDGDTMMRFLPCYQVVESMR-QGMKPEEAAKDAISRIA 313

The following BLAST results are available for this feature:
BLAST of Carg25041 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7022301.14.9e-145100.00Isoaspartyl peptidase/L-asparaginase 1, partial [Cucurbita argyrosperma subsp. a... [more]
KAG6588471.14.9e-145100.00Isoaspartyl peptidase/L-asparaginase 1, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023530268.16.6e-14298.50isoaspartyl peptidase/L-asparaginase 1 [Cucurbita pepo subsp. pepo][more]
XP_022969815.14.3e-14198.12isoaspartyl peptidase/L-asparaginase 1 [Cucurbita maxima][more]
XP_022933679.11.6e-14097.74isoaspartyl peptidase/L-asparaginase 1 [Cucurbita moschata][more]
BLAST of Carg25041 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P502878.7e-11381.13Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08... [more]
P303641.7e-10877.32Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1[more]
P303625.0e-10877.99Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2... [more]
Q9ZSD62.5e-10776.21Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1[more]
P502882.1e-10676.21Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1[more]
BLAST of Carg25041 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1I1052.1e-14198.12isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita maxima OX=3661 GN=LOC1114689... [more]
A0A6J1F0F17.9e-14197.74isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1DSP76.9e-12991.22isoaspartyl peptidase/L-asparaginase 1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5A7UBC61.7e-12790.84Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3CEE46.5e-12790.46Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
BLAST of Carg25041 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G08100.16.2e-11481.13N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.24.2e-10281.25N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT3G16150.12.3e-8461.11N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.11.2e-1626.67N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G61540.19.5e-1428.22N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 1..260
e-value: 1.1E-98
score: 330.1
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 1..260
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 132..263
e-value: 3.2E-45
score: 155.5
NoneNo IPR availablePANTHERPTHR10188:SF33ISOASPARTYL PEPTIDASE/L-ASPARAGINASEcoord: 1..260
NoneNo IPR availableCDDcd04701Asparaginase_2coord: 1..253
e-value: 7.0778E-113
score: 323.644
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 1..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25041-RACarg25041-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016540 protein autoprocessing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004067 asparaginase activity
molecular_function GO:0008798 beta-aspartyl-peptidase activity
molecular_function GO:0016787 hydrolase activity