Carg24991 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24991
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionGlycerol-3-phosphate dehydrogenase [NAD(+)]
LocationCarg_Chr03: 448064 .. 452571 (+)
RNA-Seq ExpressionCarg24991
SyntenyCarg24991
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCTCTCACATTCAAATGGTTCAAGCCATAACTTCAATGGATTTGAAGAGAAGCTTGATGAACTTAGAAGGCTTCTAGGAAAGTCCGATGGTGATCCACTAAGGATCGTTGGTGTGGGAGCTGGAGCTTGGGGGAGTGTTTTTGCGGCACTTCTTCAAGACAGCTATGGCCAATTTCGAGAGAAGGTTCAAATCAGAATATGGCGAAGAGCAGGAAGGATTCTGGATAAAGCCACAGCTGAACACCTCTTTGAAGTGATCAATTCAAGGGAAGATGTGCTAAGGAGGCTTATTCGACGATGTGCTTATCTAAAGTACGTCGAAGCGAGGCTCGGTGATCGAGTTCTCTACGCCGATGAGATTTTGAAAGATGGGTTTTGCTTGAACATGATTGACACACCACTTTGTCCTTTGAAAGTGGTGACTAATTTGCAGGAGGCTGTTTGGGATGCTGACATTGTTGTTAATGGCTTACCTTCCACTGAAACCAGAGAGGTTTTTGAAGAGATAAGTAAATATTGGAAAGAACGAATCACAGTGCCTATTATCATCTCTTTAGCTAAGGGTATAGAAGCTGCTCTTCAGCCTGTTCCTCACATAATAACTCCTACTCTGATGATCAACCGTGCAAGTAAGATATTCTTACCAAATTTGGCATTTAATCTGTTCTATTTGCATTGTGTTCTTCATTTATAAACAGATAAGAACATTTCGAGCTGAAAACGAGTTATTGTCTGTCTGGATTTGAGTATTCGACTTCATTTATAAAGATTGAGGAAGTTTAGACTAATAAGTATGGAGAACATATCGGTTGGAGAGGGGAACAAATCATTCCACATAAGGTTGTGGAAACCTCTCCCTCAGACGCGTTTTAAAATCGTGAGGCTGATGGTGATACGTAACGGGTCAAAGCGGACAATATCTGCTAGCGGTGGGCTTAGGTTATTACAAATGGTATCAGAGCCAGACACTGGGCGGTGTGCTAGAGAGGACGTTGGGCCCCCAAGGGGGGTGGATTGTGAGATCCCACGTCGCTTGGAGAGGAGAACAAAGCATTCCTCAAAAGGATGTGGAAATCTCTTTCTTGTAGACACGTTTTAAAACCGTGAGGCTAACATCGGTTTGTGTTTGTGAAATTGTTTTTCTCAGCTGGAGTGCCTATAGAGAACATATTGTATCTTGGGGGACCGAACATCGCCTCGGAGATTTATAACAAGGAATACGCGAATGCTCGGATTTGTGGAGCTGAGAAGTGGAGGAAGCCTCTTGCAAATTTTTTGAGACAACCTCAGTTTATTGTGTGGGATAATAGTGACCTTGTGACACATGAAGTCATGGGTGGCTTGAAGAACGTCTACGCCATTGGAGCTGGTAAGTGGTAGATACTTTCTGGTTAATTTATCTTAATGATGATGGAAACAGTCTTTGATATATCACTTTGTTGTAGCAATTGCTTTTGTTCTTGTTCACGTCCATTTTAGTTCATTGCAATGTGGAGTTAATCACTGTTTTTGGTAGCACCAGCGCTCTGGTGTTGGTGTAGTAGCTGTTTGACTGAAAATTGCAACCTTTTGCAATTGTGAATAGTTAGATTGTGAGATCCCACGTCAGTTAGAGAGGGGAACAAAGTATTCCTCGTAAGGGTGTGGAAACTTCTCCCTCGGATACATGTTTTAAAACTGTAAGGATGATGGTGATACGTAACGGGTTAAAGAGGATGATATCTGCTAGTGGTGGGGTTCGGCTGTTACAAATGGTATAATCAGAGCCAGACACTGGGCGGTGTGTCAGTGAGGACGCTGAGCCCCCCAAAGGGGTGGATTGTGAAATCCCACATCGGTTGGAGAGGGGAACAAAACATTCTTCATGAGGGTGTGGAGACCTCTCTCTCGTGGACGTGTTTTAAAATCATGATTAGGCGATGCGTAATGGGTCAAAGCGGACAATATCTGGTAGGAGTGGGCTTAGGCTGTTATAAATGGTATCAGAGTCAGATACTTTACGGTGTGCTAGTGAGGACACTGGGCCCTCAAGGGGGGGATTGTGAGATCCCAGGCTGGAGAGGGGAACAAAGCATTCTTCATAAGAGTGTAGAAACCTCTCCCTCGTAGACGTGTTTTAAAATCGTGATTACAGCGATACGTAATAGGTCAAAGCGGACAATATCTGCTAGCAGTGGGCTTAGGCTGTTGCAAATGTTATCAAAGCCAAACACCAGGCGGTGACCAGTGAGGATGTTGGGCCCCAAGGGAGGTGCATTGTGAGATCCCAAATCGGTTGGAGAGGTGAACAAAACATTCCTCATAAGGTTGTGAAAACCTCTCGCTTGTAGTCGCATTTTAAAACCGTGAGGCTGACGACGATACGTAACTGACCAAAGCAGACAATATCTGCTAGCGGTGGGCTTAAACTGTTACATAGATCTTTTTCATTTCTATCATTATCATCGTACATTCACGACATTCTCTCTCTTTCTCGAAAATGTGATTGTCTTAGTGATCCAGATTTTGCATTGGACAATTTTGGGACTGTTGCTTGAAGTTTTCTACCATGTTCATTGCTTTCCATTTTGAACTTTCAGGAATGGTGGCAGCCCTTACCAATGAAAGTGCTACCAGTAAATCAGTATATTTTGCACATTGCACCTCAGAGATGATCTTCATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTAGCGGGGCCTTTGCTCGCTGACACTTACGTGACCTTGTTGAAAGGTCGTAACGCTTGGTACGGGCAAATGTTGGCCAAGGGCGAACTTAGTCTAGATATGGGGGATAGCATTAGTGGCAAAGGGATGATTCAGGTAAAGAAACTGCAGTTCTTTCTTGCTCCATTATGAGTTCATGTTAGAATCACCTGTATTTTCTTGTTTAAATGCTTTACCGACTGTTTTCGAAGACTGTTTTCGAAGCACTGATTCGGAAAGAAATTCTCCTATTTCAGTACTGATATTGGGCGTGCACACACAAACTCACTAAAATGTATACGTATCTATGCTATGCACGCACATGACATTTATCTACGTACACACACGAACTCACTAAAATACATATGTATCTACGTACACACACTCGGTGAACTCACTGAAATGCATATGTATCTATGTTCACACGCTCTCTAAAATACATACGTACGTATCTAGGTTCAAGCACGAATTCTCTAAAATACACATGTATCTATGTACATACACGTGAACTCACTGAAATGCACACATATCTATGTTCACACACGAACTCACTAAAATACATATGTAGATACATACACACGTGAACTCACTGAAATACATACGTATCTATGTTCACACACAAACTCAAATACATACATACCTATGTACACATACGAACTCACAAAAATACATACGTATCTACGTGCACATACGAACTCCCTAAAATACATATGTATCTACGAATATACGCACAACATATAGTGCTAAACAATCCTTTTCTGTTTTGTAGGGAGTCTCTGCTGTTGGTGCTTTCTATGAAATTCTGAGTCAGTCAAGCCTAAACGTGTTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGCAGCTTTGTCCCATCTTAAAGACACTCTATAAAATACTTATTAAAAGGTAATTGCCTCAACTTACTATTCTAAAATTTGCATCAAATAAGTGGCCTGCAGAAATTGATATGACATTTACTTCGAACTGAATCGAACCTAATCTAAGCTTAAACTCGAAGTTTTTCAATCACGATTTCTGTTTTCTCGTGTTTCGGGTCATTAGGGATGGATCACCTCAAGCTATACTAGAAGCCTTGCGAGACGAGACCTTGAATGATCCTCGAGACCGGATCGAGTTGGCACAAAGCCATGCTTTCTACAGGCCTTCACTTCTTGGTCAACCTTGAACTGTAGATTCCATGGCAAAACTTAAAACTAGTCTGAAAGAGTGAGCCCTGCATAAATGAGCTATGAACTAGTCTATAGGTTTCTTTCTTGGTCCTATAGACAATTATATGTCACATGGAGAAAGTTATATGATAATGCTGAATTTCAAATTATTTCTTGGCACTTTGCTTTTGAAAGGGAATCAATATCAAAATTCTCTCATCTTTGATACAGTGAATCTTTTATGAATTCGAATCTTATTTCGGTTTTCGAACTTTTAATACTCGTTTCATTTTGGTTTAGTTATATTCCTATATATGAACCGTTTTGTAAAAATTCTGGGTAGCTTTCTGAATGTTCTTGGATATCCTCCATGTAGCAAGTGCTTAAAACATGTATAATGGTTTTGAAATGGGATCCAAACTTCTAATATAGATAAGAAACTTCGAAAACTATGTTTGTTTTCTCACACCTTTCTCATTTCAATTCTTTCTTCTTAACTTGGAACACAAAAAAAAGCTATTCAACTTGGTCAGCTAAACTCAAAGTTATAACCTCTCAAAGTTTAAACTTATTTAAGATATCACCAAAGCCATTATAAGACGAACTCCTGTAAAATTTTGG

mRNA sequence

ATGAATCTCTCACATTCAAATGGTTCAAGCCATAACTTCAATGGATTTGAAGAGAAGCTTGATGAACTTAGAAGGCTTCTAGGAAAGTCCGATGGTGATCCACTAAGGATCGTTGGTGTGGGAGCTGGAGCTTGGGGGAGTGTTTTTGCGGCACTTCTTCAAGACAGCTATGGCCAATTTCGAGAGAAGGTTCAAATCAGAATATGGCGAAGAGCAGGAAGGATTCTGGATAAAGCCACAGCTGAACACCTCTTTGAAGTGATCAATTCAAGGGAAGATGTGCTAAGGAGGCTTATTCGACGATGTGCTTATCTAAAGTACGTCGAAGCGAGGCTCGGTGATCGAGTTCTCTACGCCGATGAGATTTTGAAAGATGGGTTTTGCTTGAACATGATTGACACACCACTTTGTCCTTTGAAAGTGGTGACTAATTTGCAGGAGGCTGTTTGGGATGCTGACATTGTTGTTAATGGCTTACCTTCCACTGAAACCAGAGAGGTTTTTGAAGAGATAAGTAAATATTGGAAAGAACGAATCACAGTGCCTATTATCATCTCTTTAGCTAAGGGTATAGAAGCTGCTCTTCAGCCTGTTCCTCACATAATAACTCCTACTCTGATGATCAACCGTGCAATCATGGGTGGCTTGAAGAACGTCTACGCCATTGGAGCTGGAATGGTGGCAGCCCTTACCAATGAAAGTGCTACCAGTAAATCAGTATATTTTGCACATTGCACCTCAGAGATGATCTTCATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTAGCGGGGCCTTTGCTCGCTGACACTTACGTGACCTTGTTGAAAGGTCGTAACGCTTGGTACGGGCAAATGTTGGCCAAGGGCGAACTTAGTCTAGATATGGGGGATAGCATTAGTGGCAAAGGGATGATTCAGGGAGTCTCTGCTGTTGGTGCTTTCTATGAAATTCTGAGTCAGTCAAGCCTAAACGTGTTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGCAGCTTTGTCCCATCTTAAAGACACTCTATAAAATACTTATTAAAAGGGATGGATCACCTCAAGCTATACTAGAAGCCTTGCGAGACGAGACCTTGAATGATCCTCGAGACCGGATCGAGTTGGCACAAAGCCATGCTTTCTACAGGCCTTCACTTCTTGGTCAACCTTGAACTGTAGATTCCATGGCAAAACTTAAAACTAGTCTGAAAGAGTGAGCCCTGCATAAATGAGCTATGAACTAGTCTATAGGTTTCTTTCTTGGTCCTATAGACAATTATATGTCACATGGAGAAAGTTATATGATAATGCTGAATTTCAAATTATTTCTTGGCACTTTGCTTTTGAAAGGGAATCAATATCAAAATTCTCTCATCTTTGATACAGTGAATCTTTTATGAATTCGAATCTTATTTCGGTTTTCGAACTTTTAATACTCGTTTCATTTTGGTTTAGTTATATTCCTATATATGAACCGTTTTGTAAAAATTCTGGGTAGCTTTCTGAATGTTCTTGGATATCCTCCATGTAGCAAGTGCTTAAAACATGTATAATGGTTTTGAAATGGGATCCAAACTTCTAATATAGATAAGAAACTTCGAAAACTATGTTTGTTTTCTCACACCTTTCTCATTTCAATTCTTTCTTCTTAACTTGGAACACAAAAAAAAGCTATTCAACTTGGTCAGCTAAACTCAAAGTTATAACCTCTCAAAGTTTAAACTTATTTAAGATATCACCAAAGCCATTATAAGACGAACTCCTGTAAAATTTTGG

Coding sequence (CDS)

ATGAATCTCTCACATTCAAATGGTTCAAGCCATAACTTCAATGGATTTGAAGAGAAGCTTGATGAACTTAGAAGGCTTCTAGGAAAGTCCGATGGTGATCCACTAAGGATCGTTGGTGTGGGAGCTGGAGCTTGGGGGAGTGTTTTTGCGGCACTTCTTCAAGACAGCTATGGCCAATTTCGAGAGAAGGTTCAAATCAGAATATGGCGAAGAGCAGGAAGGATTCTGGATAAAGCCACAGCTGAACACCTCTTTGAAGTGATCAATTCAAGGGAAGATGTGCTAAGGAGGCTTATTCGACGATGTGCTTATCTAAAGTACGTCGAAGCGAGGCTCGGTGATCGAGTTCTCTACGCCGATGAGATTTTGAAAGATGGGTTTTGCTTGAACATGATTGACACACCACTTTGTCCTTTGAAAGTGGTGACTAATTTGCAGGAGGCTGTTTGGGATGCTGACATTGTTGTTAATGGCTTACCTTCCACTGAAACCAGAGAGGTTTTTGAAGAGATAAGTAAATATTGGAAAGAACGAATCACAGTGCCTATTATCATCTCTTTAGCTAAGGGTATAGAAGCTGCTCTTCAGCCTGTTCCTCACATAATAACTCCTACTCTGATGATCAACCGTGCAATCATGGGTGGCTTGAAGAACGTCTACGCCATTGGAGCTGGAATGGTGGCAGCCCTTACCAATGAAAGTGCTACCAGTAAATCAGTATATTTTGCACATTGCACCTCAGAGATGATCTTCATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTAGCGGGGCCTTTGCTCGCTGACACTTACGTGACCTTGTTGAAAGGTCGTAACGCTTGGTACGGGCAAATGTTGGCCAAGGGCGAACTTAGTCTAGATATGGGGGATAGCATTAGTGGCAAAGGGATGATTCAGGGAGTCTCTGCTGTTGGTGCTTTCTATGAAATTCTGAGTCAGTCAAGCCTAAACGTGTTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGCAGCTTTGTCCCATCTTAAAGACACTCTATAAAATACTTATTAAAAGGGATGGATCACCTCAAGCTATACTAGAAGCCTTGCGAGACGAGACCTTGAATGATCCTCGAGACCGGATCGAGTTGGCACAAAGCCATGCTTTCTACAGGCCTTCACTTCTTGGTCAACCTTGA

Protein sequence

MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAALQPVPHIITPTLMINRAIMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Homology
BLAST of Carg24991 vs. NCBI nr
Match: KAG7033505.1 (putative glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 781.2 bits (2016), Expect = 4.3e-222
Identity = 394/394 (100.00%), Postives = 394/394 (100.00%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRAIMGGLKNVYAIGAGMVAALTNESATSKSV 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRAIMGGLKNVYAIGAGMVAALTNESATSKSV
Sbjct: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRAIMGGLKNVYAIGAGMVAALTNESATSKSV 240

Query: 241 YFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSI 300
           YFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSI
Sbjct: 241 YFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSI 300

Query: 301 SGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQA 360
           SGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQA
Sbjct: 301 SGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQA 360

Query: 361 ILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 395
           ILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 361 ILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 394

BLAST of Carg24991 vs. NCBI nr
Match: KAG6603195.1 (putative glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 753.1 bits (1943), Expect = 1.2e-213
Identity = 393/455 (86.37%), Postives = 394/455 (86.59%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 38  MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 97

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 98  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 157

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 158 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 217

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRA----------------------------- 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRA                             
Sbjct: 218 VPIIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANAR 277

Query: 241 --------------------------------IMGGLKNVYAIGAGMVAALTNESATSKS 300
                                           +MGGLKNVYAIGAGMVAALTNESATSKS
Sbjct: 278 ICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKS 337

Query: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360
           VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS
Sbjct: 338 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 397

Query: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 395
           ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ
Sbjct: 398 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 457

BLAST of Carg24991 vs. NCBI nr
Match: XP_023544218.1 (probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 751.9 bits (1940), Expect = 2.8e-213
Identity = 392/455 (86.15%), Postives = 394/455 (86.59%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGS+HNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 38  MNLSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 97

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 98  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 157

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 158 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 217

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRA----------------------------- 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRA                             
Sbjct: 218 VPIIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANAR 277

Query: 241 --------------------------------IMGGLKNVYAIGAGMVAALTNESATSKS 300
                                           +MGGLKNVYAIGAGMVAALTNESATSKS
Sbjct: 278 ICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKS 337

Query: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360
           VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS
Sbjct: 338 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 397

Query: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 395
           ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ
Sbjct: 398 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 457

BLAST of Carg24991 vs. NCBI nr
Match: XP_022967787.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita maxima] >XP_022967788.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita maxima] >XP_023544219.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita pepo subsp. pepo] >XP_023544220.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 751.9 bits (1940), Expect = 2.8e-213
Identity = 392/455 (86.15%), Postives = 394/455 (86.59%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGS+HNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 1   MNLSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRA----------------------------- 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRA                             
Sbjct: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANAR 240

Query: 241 --------------------------------IMGGLKNVYAIGAGMVAALTNESATSKS 300
                                           +MGGLKNVYAIGAGMVAALTNESATSKS
Sbjct: 241 ICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKS 300

Query: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360
           VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS
Sbjct: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360

Query: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 395
           ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ
Sbjct: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 420

BLAST of Carg24991 vs. NCBI nr
Match: XP_022967785.1 (probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita maxima] >XP_022967786.1 probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita maxima])

HSP 1 Score: 751.9 bits (1940), Expect = 2.8e-213
Identity = 392/455 (86.15%), Postives = 394/455 (86.59%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGS+HNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 38  MNLSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 97

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 98  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 157

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 158 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 217

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRA----------------------------- 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRA                             
Sbjct: 218 VPIIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANAR 277

Query: 241 --------------------------------IMGGLKNVYAIGAGMVAALTNESATSKS 300
                                           +MGGLKNVYAIGAGMVAALTNESATSKS
Sbjct: 278 ICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKS 337

Query: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360
           VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS
Sbjct: 338 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 397

Query: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 395
           ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ
Sbjct: 398 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 457

BLAST of Carg24991 vs. ExPASy Swiss-Prot
Match: O22216 (Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic OS=Arabidopsis thaliana OX=3702 GN=GPDHC1 PE=2 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 7.9e-187
Identity = 341/451 (75.61%), Postives = 366/451 (81.15%), Query Frame = 0

Query: 6   SNGSSHNFN-GFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 65
           SNGS H+     EEKLDE RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 66  QIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 125
           QIRIWRRAGR +D+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 126 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 185
           DGFCLNM+DTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 186 ISLAKGIEAALQPVPHIITPTLMINRA--------------------------------- 245
           ISL+KGIE AL+PVPHIITPT MI++A                                 
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 246 ----------------------------IMGGLKNVYAIGAGMVAALTNESATSKSVYFA 305
                                       +MGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 311

Query: 306 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 365
           HCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISGK
Sbjct: 312 HCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGK 371

Query: 366 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 395
           GMIQGVSAVGAFY++LSQSSL++L  E+ KPVAPV+ CPILKTLYKILI R+ S QAIL+
Sbjct: 372 GMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAILQ 431

BLAST of Carg24991 vs. ExPASy Swiss-Prot
Match: Q8S0G4 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0939600 PE=2 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 2.0e-185
Identity = 335/451 (74.28%), Postives = 366/451 (81.15%), Query Frame = 0

Query: 5   HSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 64
           H NGS +  NG EE+LDELRRLLGKSDGD L+IVG+GAGAWGSVFAALLQD+YG+FREKV
Sbjct: 6   HVNGSVNGGNGTEERLDELRRLLGKSDGDLLKIVGIGAGAWGSVFAALLQDAYGRFREKV 65

Query: 65  QIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 124
           QIRIWRRAGR +D+ TAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDR LYADEIL+
Sbjct: 66  QIRIWRRAGRSVDRTTAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRTLYADEILR 125

Query: 125 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 184
           DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERI+VP+I
Sbjct: 126 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERISVPVI 185

Query: 185 ISLAKGIEAALQPVPHIITPTLMINRA--------------------------------- 244
           ISLAKGIEA+L P+P IITPT MI+ A                                 
Sbjct: 186 ISLAKGIEASLDPIPRIITPTQMISSATGVPTENILYLGGPNIASEIYNKEYANARICGS 245

Query: 245 ----------------------------IMGGLKNVYAIGAGMVAALTNESATSKSVYFA 304
                                       +MGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 246 NKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 305

Query: 305 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 364
           HCTSEMIFIT+LL E+PEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELS DMGDSI GK
Sbjct: 306 HCTSEMIFITHLLTEQPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSPDMGDSIKGK 365

Query: 365 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 395
           GMIQG+SAVGAF+E+LSQ SL+V HPE+NK VAP +LCPILK LY+ILIKR+ S + IL+
Sbjct: 366 GMIQGISAVGAFFELLSQPSLSVQHPEENKQVAPAELCPILKRLYRILIKRELSTRDILQ 425

BLAST of Carg24991 vs. ExPASy Swiss-Prot
Match: Q8S2G5 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0801600 PE=2 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 1.8e-175
Identity = 316/451 (70.07%), Postives = 353/451 (78.27%), Query Frame = 0

Query: 5   HSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 64
           H NG++      EEKLDELRRLLGK+DGDPLRIVGVGAGAWGSVF AL+QD+YG  R+KV
Sbjct: 16  HGNGAT-----VEEKLDELRRLLGKADGDPLRIVGVGAGAWGSVFCALMQDAYGHLRDKV 75

Query: 65  QIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 124
           Q+RIWRR GR +D+ATAEHLFEVIN+REDVLRRLIRRCAYLKYVE RLGDR LYADEIL+
Sbjct: 76  QVRIWRRPGRAVDRATAEHLFEVINAREDVLRRLIRRCAYLKYVEGRLGDRTLYADEILR 135

Query: 125 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 184
           DGFCLNMIDTPLCPLKVVTNLQEAVWDADIV+NGLPST+TREVF EI +YWKERIT PII
Sbjct: 136 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVINGLPSTDTREVFGEIGRYWKERITAPII 195

Query: 185 ISLAKGIEAALQPVPHIITPTLMINRA--------------------------------- 244
           +SLAKGIEA+L P+P IITPT MI+ A                                 
Sbjct: 196 LSLAKGIEASLDPLPRIITPTQMISNATGVPLENILYLGGPNIASEIYNKEYANARICGA 255

Query: 245 ----------------------------IMGGLKNVYAIGAGMVAALTNESATSKSVYFA 304
                                       +MGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 256 DKWRKPLAKFLRQPHFIVWDNSDLITHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 315

Query: 305 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 364
            CTSEMI+IT+LL EEPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGEL+L+MGDSI GK
Sbjct: 316 LCTSEMIYITHLLEEEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELTLEMGDSIKGK 375

Query: 365 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 395
           G IQGVSAV AFYE+LSQ SL+V+HPE N+ VAPV++CPILK LYKILIKR+  P +IL+
Sbjct: 376 GTIQGVSAVNAFYELLSQDSLSVMHPEANRSVAPVEMCPILKALYKILIKRELPPDSILQ 435

BLAST of Carg24991 vs. ExPASy Swiss-Prot
Match: Q9S785 (Glycerol-3-phosphate dehydrogenase [NAD(+)] At3g07690, cytosolic OS=Arabidopsis thaliana OX=3702 GN=At3g07690 PE=3 SV=2)

HSP 1 Score: 599.7 bits (1545), Expect = 2.3e-170
Identity = 304/438 (69.41%), Postives = 345/438 (78.77%), Query Frame = 0

Query: 17  EEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRIL 76
           EE+LDE+RR++GK+D DPLRIVGVGAGAWGSVF A+LQ++YG+FR KV +RIWRR GR +
Sbjct: 17  EERLDEIRRVMGKADDDPLRIVGVGAGAWGSVFIAMLQENYGKFRGKVSVRIWRRGGRAI 76

Query: 77  DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTPL 136
           DKATAEHLFEVINSRE++LRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMI+TPL
Sbjct: 77  DKATAEHLFEVINSREELLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIETPL 136

Query: 137 CPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAALQ 196
           CPLKVVTNLQEAVWDADIV+NGLPSTET +VF EISKYWKER+  P+IISLAKG+EA  +
Sbjct: 137 CPLKVVTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEFE 196

Query: 197 PVPHIITPTLMINRA--------------------------------------------- 256
           P P I+TPT MI+RA                                             
Sbjct: 197 PHPRIVTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFLR 256

Query: 257 ----------------IMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFITYL 316
                           +MGGLKNVYAIGAGMVA LT ESATSKSVYFAHCTSEMIFIT+L
Sbjct: 257 QSHFIVWDNSDLITHEVMGGLKNVYAIGAGMVATLTKESATSKSVYFAHCTSEMIFITHL 316

Query: 317 LAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVGAF 376
           LA+EPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGELSL+MGDSI GKGMIQGVSAV AF
Sbjct: 317 LAKEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELSLEMGDSIKGKGMIQGVSAVKAF 376

Query: 377 YEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILEALRDETLNDPRD 394
           +E+L+QSSL++ HPE+ KPV P +LCPILK LY+ILI R+ S +AILEALRDET+NDPR+
Sbjct: 377 FELLNQSSLSLQHPEEGKPVTPAELCPILKMLYRILITREFSCEAILEALRDETMNDPRE 436

BLAST of Carg24991 vs. ExPASy Swiss-Prot
Match: Q65X70 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0495700 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.1e-167
Identity = 303/439 (69.02%), Postives = 341/439 (77.68%), Query Frame = 0

Query: 17  EEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRIL 76
           E KLDELRR +GK+DGD LRIVGVG GAWGS F ALLQD+YG+ R+K Q+R+WRR GR +
Sbjct: 20  EGKLDELRRRMGKADGDLLRIVGVGGGAWGSAFCALLQDAYGRHRDKAQVRVWRRPGRAV 79

Query: 77  DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTPL 136
           D+ATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDR LYADEIL+DGFCLNM+DTPL
Sbjct: 80  DRATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRTLYADEILRDGFCLNMVDTPL 139

Query: 137 CPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAALQ 196
           CPLKVVTNLQEAVWDADIV+NGLPSTETREVF EI +YWKERI  P+IISLAKGIEA++ 
Sbjct: 140 CPLKVVTNLQEAVWDADIVINGLPSTETREVFGEIGRYWKERIRPPVIISLAKGIEASID 199

Query: 197 PVPHIITPTLMINRA--------------------------------------------- 256
           PVP IITPT MI+ A                                             
Sbjct: 200 PVPRIITPTQMISNATGVPLENILYLGGPNIASEIYNKEYANARICGADKWRKPLAKFLR 259

Query: 257 ----------------IMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFITYL 316
                           +MGGLKN+YAIGAGMVAALTNESATSKSVYF+ CTSEMI+IT+L
Sbjct: 260 QPHFIVWDNSDLITHEVMGGLKNIYAIGAGMVAALTNESATSKSVYFSLCTSEMIYITHL 319

Query: 317 LAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVGAF 376
           LAE+PEKLAGPLLADTYVTLLKGRNAWYGQ LAKGEL+L+MGDSI GKG IQGVSAV AF
Sbjct: 320 LAEDPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELTLEMGDSIKGKGTIQGVSAVHAF 379

Query: 377 YEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILEALRDETLNDPRD 395
           YE+LSQSSL+V HPE  K VAPV+LCPILKTLYKILIKR+ +  +IL+A+RDE++ DPR+
Sbjct: 380 YELLSQSSLSVTHPEVKKLVAPVELCPILKTLYKILIKRELATDSILQAIRDESMYDPRE 439

BLAST of Carg24991 vs. ExPASy TrEMBL
Match: A0A6J1HW44 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111467198 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.3e-213
Identity = 392/455 (86.15%), Postives = 394/455 (86.59%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGS+HNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 1   MNLSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRA----------------------------- 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRA                             
Sbjct: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANAR 240

Query: 241 --------------------------------IMGGLKNVYAIGAGMVAALTNESATSKS 300
                                           +MGGLKNVYAIGAGMVAALTNESATSKS
Sbjct: 241 ICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKS 300

Query: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360
           VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS
Sbjct: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360

Query: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 395
           ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ
Sbjct: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 420

BLAST of Carg24991 vs. ExPASy TrEMBL
Match: A0A6J1HVE6 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111467198 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.3e-213
Identity = 392/455 (86.15%), Postives = 394/455 (86.59%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGS+HNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 38  MNLSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 97

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 98  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 157

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 158 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 217

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRA----------------------------- 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRA                             
Sbjct: 218 VPIIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANAR 277

Query: 241 --------------------------------IMGGLKNVYAIGAGMVAALTNESATSKS 300
                                           +MGGLKNVYAIGAGMVAALTNESATSKS
Sbjct: 278 ICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKS 337

Query: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360
           VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS
Sbjct: 338 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 397

Query: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 395
           ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ
Sbjct: 398 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 457

BLAST of Carg24991 vs. ExPASy TrEMBL
Match: A0A6J1ELJ7 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC111435526 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.0e-213
Identity = 391/455 (85.93%), Postives = 394/455 (86.59%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGS+HNFNGFEEKLDELRR+LGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 1   MNLSHSNGSTHNFNGFEEKLDELRRVLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRA----------------------------- 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRA                             
Sbjct: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRATRVPIENILYLGGPNIASEIYNKEYANAR 240

Query: 241 --------------------------------IMGGLKNVYAIGAGMVAALTNESATSKS 300
                                           +MGGLKNVYAIGAGMVAALTNESATSKS
Sbjct: 241 ICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKS 300

Query: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360
           VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS
Sbjct: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360

Query: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 395
           ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ
Sbjct: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 420

BLAST of Carg24991 vs. ExPASy TrEMBL
Match: A0A6J1EPS2 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC111435526 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.0e-213
Identity = 391/455 (85.93%), Postives = 394/455 (86.59%), Query Frame = 0

Query: 1   MNLSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 60
           MNLSHSNGS+HNFNGFEEKLDELRR+LGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF
Sbjct: 38  MNLSHSNGSTHNFNGFEEKLDELRRVLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQF 97

Query: 61  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 120
           REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD
Sbjct: 98  REKVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYAD 157

Query: 121 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 180
           EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT
Sbjct: 158 EILKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERIT 217

Query: 181 VPIIISLAKGIEAALQPVPHIITPTLMINRA----------------------------- 240
           VPIIISLAKGIEAALQPVPHIITPTLMINRA                             
Sbjct: 218 VPIIISLAKGIEAALQPVPHIITPTLMINRATRVPIENILYLGGPNIASEIYNKEYANAR 277

Query: 241 --------------------------------IMGGLKNVYAIGAGMVAALTNESATSKS 300
                                           +MGGLKNVYAIGAGMVAALTNESATSKS
Sbjct: 278 ICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKS 337

Query: 301 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 360
           VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS
Sbjct: 338 VYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDS 397

Query: 361 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 395
           ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ
Sbjct: 398 ISGKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQ 457

BLAST of Carg24991 vs. ExPASy TrEMBL
Match: A0A6J1DI67 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LOC111021315 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.2e-207
Identity = 380/453 (83.89%), Postives = 388/453 (85.65%), Query Frame = 0

Query: 3   LSHSNGSSHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 62
           LSHSNGS+HN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE
Sbjct: 6   LSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 65

Query: 63  KVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 122
           KVQIRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI
Sbjct: 66  KVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 125

Query: 123 LKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVP 182
           LKDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVP
Sbjct: 126 LKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVP 185

Query: 183 IIISLAKGIEAALQPVPHIITPTLMINRA------------------------------- 242
           IIISLAKGIEAAL+PVPHIITPTLMINRA                               
Sbjct: 186 IIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 245

Query: 243 ------------------------------IMGGLKNVYAIGAGMVAALTNESATSKSVY 302
                                         +MGGLKNVYAIGAGMVAALTNESATSKSVY
Sbjct: 246 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 305

Query: 303 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 362
           FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS
Sbjct: 306 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 365

Query: 363 GKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAI 395
           GKGMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAI
Sbjct: 366 GKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAI 425

BLAST of Carg24991 vs. TAIR 10
Match: AT2G41540.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 654.4 bits (1687), Expect = 5.6e-188
Identity = 341/451 (75.61%), Postives = 366/451 (81.15%), Query Frame = 0

Query: 6   SNGSSHNFN-GFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 65
           SNGS H+     EEKLDE RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 66  QIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 125
           QIRIWRRAGR +D+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 126 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 185
           DGFCLNM+DTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 186 ISLAKGIEAALQPVPHIITPTLMINRA--------------------------------- 245
           ISL+KGIE AL+PVPHIITPT MI++A                                 
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 246 ----------------------------IMGGLKNVYAIGAGMVAALTNESATSKSVYFA 305
                                       +MGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 311

Query: 306 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 365
           HCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISGK
Sbjct: 312 HCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGK 371

Query: 366 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 395
           GMIQGVSAVGAFY++LSQSSL++L  E+ KPVAPV+ CPILKTLYKILI R+ S QAIL+
Sbjct: 372 GMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAILQ 431

BLAST of Carg24991 vs. TAIR 10
Match: AT2G41540.2 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 654.4 bits (1687), Expect = 5.6e-188
Identity = 341/451 (75.61%), Postives = 366/451 (81.15%), Query Frame = 0

Query: 6   SNGSSHNFN-GFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 65
           SNGS H+     EEKLDE RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 66  QIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 125
           QIRIWRRAGR +D+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 126 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 185
           DGFCLNM+DTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 186 ISLAKGIEAALQPVPHIITPTLMINRA--------------------------------- 245
           ISL+KGIE AL+PVPHIITPT MI++A                                 
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 246 ----------------------------IMGGLKNVYAIGAGMVAALTNESATSKSVYFA 305
                                       +MGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 311

Query: 306 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 365
           HCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISGK
Sbjct: 312 HCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGK 371

Query: 366 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 395
           GMIQGVSAVGAFY++LSQSSL++L  E+ KPVAPV+ CPILKTLYKILI R+ S QAIL+
Sbjct: 372 GMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAILQ 431

BLAST of Carg24991 vs. TAIR 10
Match: AT2G41540.3 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 654.4 bits (1687), Expect = 5.6e-188
Identity = 341/451 (75.61%), Postives = 366/451 (81.15%), Query Frame = 0

Query: 6   SNGSSHNFN-GFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 65
           SNGS H+     EEKLDE RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 66  QIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 125
           QIRIWRRAGR +D+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 126 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 185
           DGFCLNM+DTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 186 ISLAKGIEAALQPVPHIITPTLMINRA--------------------------------- 245
           ISL+KGIE AL+PVPHIITPT MI++A                                 
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 246 ----------------------------IMGGLKNVYAIGAGMVAALTNESATSKSVYFA 305
                                       +MGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 311

Query: 306 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 365
           HCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISGK
Sbjct: 312 HCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGK 371

Query: 366 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 395
           GMIQGVSAVGAFY++LSQSSL++L  E+ KPVAPV+ CPILKTLYKILI R+ S QAIL+
Sbjct: 372 GMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAILQ 431

BLAST of Carg24991 vs. TAIR 10
Match: AT3G07690.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 591.7 bits (1524), Expect = 4.5e-169
Identity = 304/448 (67.86%), Postives = 345/448 (77.01%), Query Frame = 0

Query: 17  EEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRIL 76
           EE+LDE+RR++GK+D DPLRIVGVGAGAWGSVF A+LQ++YG+FR KV +RIWRR GR +
Sbjct: 17  EERLDEIRRVMGKADDDPLRIVGVGAGAWGSVFIAMLQENYGKFRGKVSVRIWRRGGRAI 76

Query: 77  DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTPL 136
           DKATAEHLFEVINSRE++LRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMI+TPL
Sbjct: 77  DKATAEHLFEVINSREELLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIETPL 136

Query: 137 CPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAALQ 196
           CPLKVVTNLQEAVWDADIV+NGLPSTET +VF EISKYWKER+  P+IISLAKG+EA  +
Sbjct: 137 CPLKVVTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEFE 196

Query: 197 PVPHIITPTLMINRA--------------------------------------------- 256
           P P I+TPT MI+RA                                             
Sbjct: 197 PHPRIVTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFLR 256

Query: 257 ----------------IMGGLKNVYAIGA----------GMVAALTNESATSKSVYFAHC 316
                           +MGGLKNVYAIGA          GMVA LT ESATSKSVYFAHC
Sbjct: 257 QSHFIVWDNSDLITHEVMGGLKNVYAIGAVFVLAFLYSTGMVATLTKESATSKSVYFAHC 316

Query: 317 TSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGM 376
           TSEMIFIT+LLA+EPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGELSL+MGDSI GKGM
Sbjct: 317 TSEMIFITHLLAKEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELSLEMGDSIKGKGM 376

Query: 377 IQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILEAL 394
           IQGVSAV AF+E+L+QSSL++ HPE+ KPV P +LCPILK LY+ILI R+ S +AILEAL
Sbjct: 377 IQGVSAVKAFFELLNQSSLSLQHPEEGKPVTPAELCPILKMLYRILITREFSCEAILEAL 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7033505.14.3e-222100.00putative glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic, partial [Cucu... [more]
KAG6603195.11.2e-21386.37putative glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic, partial [Cucu... [more]
XP_023544218.12.8e-21386.15probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cu... [more]
XP_022967787.12.8e-21386.15glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurb... [more]
XP_022967785.12.8e-21386.15probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cu... [more]
Match NameE-valueIdentityDescription
O222167.9e-18775.61Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic OS=Arabidopsis tha... [more]
Q8S0G42.0e-18574.28Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic OS=Oryza sativ... [more]
Q8S2G51.8e-17570.07Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic OS=Oryza sativ... [more]
Q9S7852.3e-17069.41Glycerol-3-phosphate dehydrogenase [NAD(+)] At3g07690, cytosolic OS=Arabidopsis ... [more]
Q65X701.1e-16769.02Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic OS=Oryza sativ... [more]
Match NameE-valueIdentityDescription
A0A6J1HW441.3e-21386.15Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HVE61.3e-21386.15Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1ELJ73.0e-21385.93Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1EPS23.0e-21385.93Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1DI673.2e-20783.89Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
AT2G41540.15.6e-18875.616-phosphogluconate dehydrogenase family protein [more]
AT2G41540.25.6e-18875.616-phosphogluconate dehydrogenase family protein [more]
AT2G41540.35.6e-18875.616-phosphogluconate dehydrogenase family protein [more]
AT3G07690.14.5e-16967.866-phosphogluconate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 118..211
e-value: 3.2E-7
score: 32.3
NoneNo IPR availableGENE3D3.40.50.720coord: 16..98
e-value: 7.7E-7
score: 30.7
NoneNo IPR availablePANTHERPTHR11728:SF40GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]coord: 210..394
NoneNo IPR availablePANTHERPTHR11728:SF40GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]coord: 7..211
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPIRSFPIRSF000114Glycerol-3-P_dhcoord: 29..211
e-value: 5.0E-30
score: 102.6
coord: 200..373
e-value: 1.1E-28
score: 98.2
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPANTHERPTHR11728GLYCEROL-3-PHOSPHATE DEHYDROGENASEcoord: 210..394
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPANTHERPTHR11728GLYCEROL-3-PHOSPHATE DEHYDROGENASEcoord: 7..211
IPR011128Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminalPFAMPF01210NAD_Gly3P_dh_Ncoord: 139..209
e-value: 1.6E-6
score: 28.2
IPR006109Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminalPFAMPF07479NAD_Gly3P_dh_Ccoord: 212..360
e-value: 1.9E-21
score: 76.8
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 212..367
e-value: 2.5E-30
score: 107.7
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 35..193
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 210..357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24991-RACarg24991-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0046168 glycerol-3-phosphate catabolic process
biological_process GO:0006116 NADH oxidation
biological_process GO:0006072 glycerol-3-phosphate metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0009331 glycerol-3-phosphate dehydrogenase complex
molecular_function GO:0004367 glycerol-3-phosphate dehydrogenase [NAD+] activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor