Carg24527 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24527
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	transcription factor bHLH71-like
Location	Carg_Chr19: 1719740 .. 1722705 (+)
RNA-Seq Expression	Carg24527
Synteny	Carg24527

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTCTTGATGCTCTTTCTTCCAATGACTTCTTCAACTTCATCATCTACGACACCATCTCTGCAATCCCCAACACCTCCCCCATCCTCCACGACTCGTCGGAGAACACATTTTTCTCCGACGACACGTGTTCGAGACATCAAGATCACCAACATGGGCTTGAAGGAAGCTTGAAACCCGATAGAAAATGCCGTCCGAGTGAGTCGGAAAGCCTCCGGACGGCGTCGACACGACAAGGGAAGAAGAAGAGGAAGAGAAAACCAAGGGTTTGTAAGAACAAAGAGGAAGCTGAAACACAAAGAATGACACACATTGCTGTTGAAAGGAACCGCCGCAAACAGATGAATGAGCATCTTTCAGTGTTACGTTCACTCATGCCCGAATCCTATGTTCAAAGGGTTTGTTTTCTTCTATTTGTCTCTTTCATTACTAAAATTTGGGACCCCATTTCTGAGATTTGATTAAACTTCATAATTATGCCATTAAAACTCCTACCCACCTTCTTCTTTTGCTCATATCTTCGTTACTAATCTCAATTATTTAGCCTAAACATAGAATTCAATTATTACTGCTAAAAATTGACCTCTGCTCCTAACCCTATTCATAATTGCATGGCTTGGCTACATTTTTGTTCAAGAACTTCAAAAAATGACAGATTCATAGGAAGCAGAGTTTGTAAGGCCCTAAAATATGATACGTACCTTTTTGATAGCAATCTTTCATGAACTGATATCAGTTTTATGTGATGAACATATGAAGGGTGACCAAGCATCCATAGTAGGAGGAGCGGTTGAGTTTGTGAAGGAATTGGAGCATCTTCTATCAACTTTGGAAGCCAAGAAGCTGCAAGTTTTACAGAAAGAAGTTGATCATCAAGAACAAGAGATTAATGATAATAAGTTGTTTTCATTTGGAAGTTTGTTGATGAATAATTCAGATCATAATTATTGTTCCCAATACTCTACCAAATACACTTCCAAATCCAAGGCTTCTTCAGCTGATATTGAAGTTACTTTGATTGAAACTCATGCAAACCTTAGAATCCTATCAACAAGAAGCCATAGACAGCTCTTGAAGCTCATTGCTGGTTTGCAGGCTCTTCGTCTCACCATTCTTCATCTAAGTCTTACTGATTTTCATCCATTGGTTCTTTACTCCATCAGCCTCAAGGTACTTACTTACCTAATGTTTCGAACTTTGTTGTGAGATCCTACGTTGGTTGAAGAGGGGAACGAAACATTCTTTATAAGGGTGTGGAAACCACTTTCTAGCAGACGCATTTTAAAAGCCTTGAGGGAAAGCTCAAAGAGGACAGTATCTGCTAGCGGTGGGCTCTGATACCATTAGATCCCACATTGGTTGGAGAGGTGTATGAAACGCTCCTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACACGTTTTACAACCTTGACGGCAAGTCCGGAATTGAAGGCCCAAAAAAGACAATATCTGCTAACAGTGGGTTTGAGCTGTTATAAAAGATATCAGAGCCAGACATCGAGCGGTGTGCCACCAAGGATGTTGGGACCCAAGGGAGGTGGATTGTGAGATCCCACGTCGCGATTGGAGAGGAAAACAAACCATTCCTTATAAGGGTGTGGAAACCTCTCCTAGTATACCCGTTTTAAAACCTTGAGGGGAAGCTCTAAATGGAAAGCCCAAAAAAAACAATATCTGCTAACAGTGGGCTTGGGCTGTTACAAATGGTATTAGAGTCAGGCACCGAGAAGTGTGTTAACGAGGACGCTGGGCCCCAAGGGGGGTGGATTGTGAGATCCCACATCGGTTGAAAAGGGGAACAAAACATTCCTTATAAGGGTGTGGAAACCTCTCTTTAGCAAACGTGTTTTAAAAACCTTGAGGGGAAGCCTGAAAGGGAAAGCCCAAAGAGAACAATATTTGCTAGCGATGAGCTTGGGCCTACACTTATCAAACTTTTCATTCTCTAATTACCATTTTATAACCGTCTCTAAACTTTCAAAATGGTCTAGATACGAAACTTCTTTATCAAAATGGAATAAGTCAAAGTTCAAAGTCGGAGTTCGGGGCAAGAATGAAGATTAAACTTACGTCGTTTTGATGAAACAATGAAAATGCAGGTTGAAGAAGGATGCCAACTGAGATCAGTAGAAGACATAGCAGCAGCAGCACATCATATGGTGAGAATAATTGAGGAAGAAACTATGTTATGTTAAAATGTAAACAATTCAAAAGCCAAAACAAAGAAAAAGAAGAGGTAAGAGCATTGAAATGGAATCAGCCATTAAAATGCCAAGTTCACTGTGTGTGCATGTGGCTTGGTTTGATGTAATTAGTAAATGAATGTGTTTTTATGTAAGATGATATTGTTTTGTAGCTTTCAAGGGTTTGTCTTATTGGCTTTAGTTTCTCTTTTTTCTTGAGACCACATACAAGAAGAGAGTGACAGATGAGAAGAAAAAGTTGTCGAAGGGATTGGTTTTGGGTTCATCATTATCTTCCTGTGCAATTTCCTCTCCCTTCCCTTCCCCTTTTCATACCTCCTTTCATCATCCAAATTTAGTTAGGGTTCTTGATGACCCACAATGCCAAATAGTGAGTAAATTTCTTTTTGGGAAGGTAAGAAGTCAATGTCCAAGATGAATAAAAGTAATTGAAACGAGAGATTTGTTTACAAAACGATGATCAGGACGGTTTAGAGAGCAATGCCACGTGCCGTGGTTATACCTTATTCCATCTATAGTCATTTAAGTTCGGGTTCTACAATGTCGTCTCATTTTAGTAGACGCATCTCTTCGTAACTTCATTCAATGGGTTTTAAACACTTAGAAAATCATTCTGACGAACAAACACGTAAGCACATAATCTATATGGTTGAACTATACTAGAAAGAATAAGTTAGGTGCGGTTGTGTAGTAGCTCAAGCCCACTGTTAGCAGATATTGTTCGCTTTGGCTCGTTACG

mRNA sequence

ATGAGTCTTGATGCTCTTTCTTCCAATGACTTCTTCAACTTCATCATCTACGACACCATCTCTGCAATCCCCAACACCTCCCCCATCCTCCACGACTCGTCGGAGAACACATTTTTCTCCGACGACACGTGTTCGAGACATCAAGATCACCAACATGGGCTTGAAGGAAGCTTGAAACCCGATAGAAAATGCCGTCCGAGTGAGTCGGAAAGCCTCCGGACGGCGTCGACACGACAAGGGAAGAAGAAGAGGAAGAGAAAACCAAGGGTTTGTAAGAACAAAGAGGAAGCTGAAACACAAAGAATGACACACATTGCTGTTGAAAGGAACCGCCGCAAACAGATGAATGAGCATCTTTCAGTGTTACGTTCACTCATGCCCGAATCCTATGTTCAAAGGGTTGAAGAAGGATGCCAACTGAGATCAGTAGAAGACATAGCAGCAGCAGCACATCATATGGTGAGAATAATTGAGGAAGAAACTATGTTATGTTAAAATGTAAACAATTCAAAAGCCAAAACAAAGAAAAAGAAGAGGTAAGAGCATTGAAATGGAATCAGCCATTAAAATGCCAAGTTCACTGTGTGTGCATGTGGCTTGGTTTGATGTAATTAGTAAATGAATGTGTTTTTATGTAAGATGATATTGTTTTGTAGCTTTCAAGGGTTTGTCTTATTGGCTTTAGTTTCTCTTTTTTCTTGAGACCACATACAAGAAGAGAGTGACAGATGAGAAGAAAAAGTTGTCGAAGGGATTGGTTTTGGGTTCATCATTATCTTCCTGTGCAATTTCCTCTCCCTTCCCTTCCCCTTTTCATACCTCCTTTCATCATCCAAATTTAGTTAGGGTTCTTGATGACCCACAATGCCAAATAGTGAGTAAATTTCTTTTTGGGAAGGTAAGAAGTCAATGTCCAAGATGAATAAAAGTAATTGAAACGAGAGATTTGTTTACAAAACGATGATCAGGACGGTTTAGAGAGCAATGCCACGTGCCGTGGTTATACCTTATTCCATCTATAGTCATTTAAGTTCGGGTTCTACAATGTCGTCTCATTTTAGTAGACGCATCTCTTCGTAACTTCATTCAATGGGTTTTAAACACTTAGAAAATCATTCTGACGAACAAACACGTAAGCACATAATCTATATGGTTGAACTATACTAGAAAGAATAAGTTAGGTGCGGTTGTGTAGTAGCTCAAGCCCACTGTTAGCAGATATTGTTCGCTTTGGCTCGTTACG

Coding sequence (CDS)

ATGAGTCTTGATGCTCTTTCTTCCAATGACTTCTTCAACTTCATCATCTACGACACCATCTCTGCAATCCCCAACACCTCCCCCATCCTCCACGACTCGTCGGAGAACACATTTTTCTCCGACGACACGTGTTCGAGACATCAAGATCACCAACATGGGCTTGAAGGAAGCTTGAAACCCGATAGAAAATGCCGTCCGAGTGAGTCGGAAAGCCTCCGGACGGCGTCGACACGACAAGGGAAGAAGAAGAGGAAGAGAAAACCAAGGGTTTGTAAGAACAAAGAGGAAGCTGAAACACAAAGAATGACACACATTGCTGTTGAAAGGAACCGCCGCAAACAGATGAATGAGCATCTTTCAGTGTTACGTTCACTCATGCCCGAATCCTATGTTCAAAGGGTTGAAGAAGGATGCCAACTGAGATCAGTAGAAGACATAGCAGCAGCAGCACATCATATGGTGAGAATAATTGAGGAAGAAACTATGTTATGTTAA

Protein sequence

MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKPDRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLSVLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETMLC

Homology

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BLAST of Carg24527 vs. NCBI nr
Match: KAG7011385.1 (Transcription factor bHLH71, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315.5 bits (807), Expect = 2.8e-82
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60
           MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP
Sbjct: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60

Query: 61  DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120
           DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS
Sbjct: 61  DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120

Query: 121 VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETMLC 165
           VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETMLC
Sbjct: 121 VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETMLC 164

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BLAST of Carg24527 vs. NCBI nr
Match: KAG6571656.1 (Transcription factor basic helix-loop-helix 94, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 253.8 bits (647), Expect = 9.9e-64
Identity = 133/162 (82.10%), Postives = 143/162 (88.27%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60
           MSLDALSSNDFFNFIIYDTISAIPNTSP+LHDSSENTFFSDDTCSRHQDHQHGLEGSLKP
Sbjct: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPVLHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60

Query: 61  DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120
           DRK RPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS
Sbjct: 61  DRKRRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120

Query: 121 VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           VLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 121 VLRSLMPESYVQRGDQASIVGGAVEFVKELEHLLSTLEAKKL 162

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BLAST of Carg24527 vs. NCBI nr
Match: XP_023554782.1 (transcription factor bHLH71-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.9 bits (642), Expect = 3.8e-63
Identity = 132/162 (81.48%), Postives = 143/162 (88.27%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60
           MSLDALSSNDFFNFIIYDTISAIPNTSP+LHDSSENTFFSDDTCSRHQDHQHGLEGSLKP
Sbjct: 62  MSLDALSSNDFFNFIIYDTISAIPNTSPVLHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 121

Query: 61  DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120
           +RK RPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS
Sbjct: 122 NRKRRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 181

Query: 121 VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           VLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 182 VLRSLMPESYVQRGDQASIVGGAVEFVKELEHLLSTLEAKKL 223

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BLAST of Carg24527 vs. NCBI nr
Match: XP_022963757.1 (transcription factor bHLH71-like [Cucurbita moschata])

HSP 1 Score: 251.5 bits (641), Expect = 4.9e-63
Identity = 132/162 (81.48%), Postives = 142/162 (87.65%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60
           MSLDALSSNDFFNFIIYDTISAIPNTSP+LHDSSENTFFSDDTCSRHQDHQHGLEGSLKP
Sbjct: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPVLHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60

Query: 61  DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120
           DRK RPSE ESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS
Sbjct: 61  DRKRRPSELESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120

Query: 121 VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           VLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 121 VLRSLMPESYVQRGDQASIVGGAVEFVKELEHLLSTLEAKKL 162

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BLAST of Carg24527 vs. NCBI nr
Match: XP_022967282.1 (transcription factor bHLH71-like [Cucurbita maxima])

HSP 1 Score: 237.3 bits (604), Expect = 9.6e-59
Identity = 126/162 (77.78%), Postives = 137/162 (84.57%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60
           MSLDALSSNDFFNFIIYDTISAIPNTS + HDSSENTFFSDDTCSRHQDHQHG EG LKP
Sbjct: 62  MSLDALSSNDFFNFIIYDTISAIPNTSLVFHDSSENTFFSDDTCSRHQDHQHGFEGCLKP 121

Query: 61  DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120
           +RK RPSESESLRTA TRQGKKKRKRKPRV KNKEEAETQRMTHIAVERNRRKQMNEHLS
Sbjct: 122 NRKRRPSESESLRTAPTRQGKKKRKRKPRVSKNKEEAETQRMTHIAVERNRRKQMNEHLS 181

Query: 121 VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           VLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 182 VLRSLMPESYVQRGDQASIVGGAVEFVKELEHLLSTLEAKKL 223

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BLAST of Carg24527 vs. ExPASy Swiss-Prot
Match: Q56XR0 (Transcription factor bHLH71 OS=Arabidopsis thaliana OX=3702 GN=BHLH71 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.6e-19
Identity = 67/162 (41.36%), Postives = 95/162 (58.64%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPI-LHDSSEN-TFFSDDTCSRHQDHQHGLEGSL 60
           M+L+ALSSN   NF++ +T+S  P  S + L    EN    S +T S   + +       
Sbjct: 1   MTLEALSSNGLLNFLLSETLSPTPFKSLVDLEPLPENDVIISKNTISEISNQE------- 60

Query: 61  KPDRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEH 120
            P ++  P         +T +GKK+R+RKPRVCKN+EEAE QRMTHIAVERNRR+QMN+H
Sbjct: 61  PPPQRQPP---------ATNRGKKRRRRKPRVCKNEEEAENQRMTHIAVERNRRRQMNQH 120

Query: 121 LSVLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEE 161
           LSVLRSLMP+ +  + ++   +    D      H +  +E +
Sbjct: 121 LSVLRSLMPQPFAHKGDQASIVGGAIDFIKELEHKLLSLEAQ 146

[Close]

BLAST of Carg24527 vs. ExPASy Swiss-Prot
Match: Q9SK91 (Transcription factor bHLH94 OS=Arabidopsis thaliana OX=3702 GN=BHLH94 PE=1 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 41/77 (53.25%), Postives = 56/77 (72.73%), Query Frame = 0


Query: 82  KKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLSVLRSLMPESYVQRVEEGCQLR 141
           ++++R+ R CKNKEE E QRMTHIAVERNRRKQMNE+L+VLRSLMP SY QR ++   + 
Sbjct: 95  RRKRRRTRNCKNKEEIENQRMTHIAVERNRRKQMNEYLAVLRSLMPSSYAQRGDQASIVG 154

Query: 142 SVEDIAAAAHHMVRIIE 159
              +      H+++ +E
Sbjct: 155 GAINYVKELEHILQSME 171

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BLAST of Carg24527 vs. ExPASy Swiss-Prot
Match: Q56YJ8 (Transcription factor FAMA OS=Arabidopsis thaliana OX=3702 GN=FAMA PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.1e-12
Identity = 44/93 (47.31%), Postives = 58/93 (62.37%), Query Frame = 0


Query: 68  ESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLSVLRSLMP 127
           E    +  + ++ K KRKR  R  K  EE E+QRMTHIAVERNRRKQMNEHL VLRSLMP
Sbjct: 164 EDRENKNVTKKEVKSKRKR-ARTSKTSEEVESQRMTHIAVERNRRKQMNEHLRVLRSLMP 223

Query: 128 ESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEE 161
            SYVQR ++   +    +       +++ +E +
Sbjct: 224 GSYVQRGDQASIIGGAIEFVRELEQLLQCLESQ 255

[Close]

BLAST of Carg24527 vs. ExPASy Swiss-Prot
Match: Q9M128 (Transcription factor bHLH57 OS=Arabidopsis thaliana OX=3702 GN=BHLH57 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-11
Identity = 50/110 (45.45%), Postives = 67/110 (60.91%), Query Frame = 0


Query: 54  LEGSLKPDRKCRPSESESLRT---ASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERN 113
           LE ++K D        +  RT   A T + K+KRKR  R  KNK+E E QRMTHIAVERN
Sbjct: 65  LETNIKRDPGQTDDPEKDPRTENGAVTVKEKRKRKR-TRAPKNKDEVENQRMTHIAVERN 124

Query: 114 RRKQMNEHLSVLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEE 161
           RR+QMNEHL+ LRSLMP S++QR ++   +    D       +++ +E E
Sbjct: 125 RRRQMNEHLNSLRSLMPPSFLQRGDQASIVGGAIDFIKELEQLLQSLEAE 173

[Close]

BLAST of Carg24527 vs. ExPASy Swiss-Prot
Match: Q9C7T4 (Transcription factor bHLH96 OS=Arabidopsis thaliana OX=3702 GN=BHLH96 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-11
Identity = 38/57 (66.67%), Postives = 47/57 (82.46%), Query Frame = 0


Query: 80  GKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLSVLRSLMPESYVQRVEE 137
           G ++++R+ R  KNKEE E QRMTHIAVERNRRKQMNE+L+VLRSLMP  Y QR ++
Sbjct: 103 GGRRKRRRTRSSKNKEEIENQRMTHIAVERNRRKQMNEYLAVLRSLMPPYYAQRGDQ 159

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BLAST of Carg24527 vs. ExPASy TrEMBL
Match: A0A6J1HG33 (transcription factor bHLH71-like OS=Cucurbita moschata OX=3662 GN=LOC111463954 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.4e-63
Identity = 132/162 (81.48%), Postives = 142/162 (87.65%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60
           MSLDALSSNDFFNFIIYDTISAIPNTSP+LHDSSENTFFSDDTCSRHQDHQHGLEGSLKP
Sbjct: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPVLHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60

Query: 61  DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120
           DRK RPSE ESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS
Sbjct: 61  DRKRRPSELESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120

Query: 121 VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           VLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 121 VLRSLMPESYVQRGDQASIVGGAVEFVKELEHLLSTLEAKKL 162

[Close]

BLAST of Carg24527 vs. ExPASy TrEMBL
Match: A0A6J1HQE1 (transcription factor bHLH71-like OS=Cucurbita maxima OX=3661 GN=LOC111466851 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 4.6e-59
Identity = 126/162 (77.78%), Postives = 137/162 (84.57%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQDHQHGLEGSLKP 60
           MSLDALSSNDFFNFIIYDTISAIPNTS + HDSSENTFFSDDTCSRHQDHQHG EG LKP
Sbjct: 62  MSLDALSSNDFFNFIIYDTISAIPNTSLVFHDSSENTFFSDDTCSRHQDHQHGFEGCLKP 121

Query: 61  DRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLS 120
           +RK RPSESESLRTA TRQGKKKRKRKPRV KNKEEAETQRMTHIAVERNRRKQMNEHLS
Sbjct: 122 NRKRRPSESESLRTAPTRQGKKKRKRKPRVSKNKEEAETQRMTHIAVERNRRKQMNEHLS 181

Query: 121 VLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           VLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 182 VLRSLMPESYVQRGDQASIVGGAVEFVKELEHLLSTLEAKKL 223

[Close]

BLAST of Carg24527 vs. ExPASy TrEMBL
Match: A0A6J1ESZ0 (transcription factor bHLH71-like OS=Cucurbita moschata OX=3662 GN=LOC111437434 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 8.5e-37
Identity = 100/168 (59.52%), Postives = 119/168 (70.83%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQD-HQHG-LEGSL 60
           MS+DALSSND FN IIYD IS  PNTS  + DSSE TFFSD+   +HQD HQHG LE   
Sbjct: 1   MSIDALSSNDLFNLIIYDAISPTPNTSHGICDSSETTFFSDEKGLKHQDNHQHGTLEHRP 60

Query: 61  KPD--RKCRPSESESLR--TASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQ 120
           KP+  RK  P+E+ES        +QGKKKRKRKP+ CKNKEEAETQRMTHIAVERNRRK 
Sbjct: 61  KPNLARKRHPNEAESSNWGAVGLQQGKKKRKRKPKACKNKEEAETQRMTHIAVERNRRKL 120

Query: 121 MNEHLSVLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           M+EHLSVLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 121 MSEHLSVLRSLMPESYVQRGDQASIVGGAVEFVKELEHVLSTLEAKKL 168

[Close]

BLAST of Carg24527 vs. ExPASy TrEMBL
Match: A0A6J1KA87 (transcription factor bHLH71-like OS=Cucurbita maxima OX=3661 GN=LOC111491514 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.6e-35
Identity = 98/168 (58.33%), Postives = 118/168 (70.24%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPILHDSSENTFFSDDTCSRHQD-HQHG-LEGSL 60
           MS+DALSSND FN IIYD IS  PNTS  + DSSE TFFS+    +HQD HQHG LE   
Sbjct: 1   MSIDALSSNDLFNLIIYDAISPTPNTSHGVCDSSETTFFSNQKGLKHQDHHQHGTLEHRP 60

Query: 61  KPD--RKCRPSESESLR--TASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQ 120
           KP+  RK  P+E+ES        +QGK+KRKRKP+ CKNKEEAETQRMTHIAVERNRRK 
Sbjct: 61  KPNLARKRHPNEAESSNWGAVGLQQGKRKRKRKPKACKNKEEAETQRMTHIAVERNRRKL 120

Query: 121 MNEHLSVLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           M+EHLSVLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 121 MSEHLSVLRSLMPESYVQRGDQASIVGGAVEFVKELEHVLSTLEAKKL 168

[Close]

BLAST of Carg24527 vs. ExPASy TrEMBL
Match: A0A0A0LF14 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G901180 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.6e-35
Identity = 97/167 (58.08%), Postives = 114/167 (68.26%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTS----PILHDSSENTFFSDDTCSRHQDHQHGLEG 60
           MSLDALSSND FNFIIYDTISA PNTS       HDSSENTF SDD CS+    +     
Sbjct: 1   MSLDALSSNDLFNFIIYDTISATPNTSNNNVVPHHDSSENTFLSDDKCSKPNSRKRCTSE 60

Query: 61  SLKPDRKCRP-SESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQM 120
               +R   P S + +  T +T+ GKKKRKRK +VCKNKEEAETQRMTHIAVERNRRKQM
Sbjct: 61  VEISNRVVVPLSTTTTTTTTTTQHGKKKRKRKAKVCKNKEEAETQRMTHIAVERNRRKQM 120

Query: 121 NEHLSVLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEETM 163
           NEHLSVLRSLMPESYVQR ++   +    +      H++  +E + +
Sbjct: 121 NEHLSVLRSLMPESYVQRGDQASIVGGAVEFVKELEHLLSTLEAKKL 167

[Close]

BLAST of Carg24527 vs. TAIR 10
Match: AT5G46690.1 (beta HLH protein 71 )

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-20
Identity = 67/162 (41.36%), Postives = 95/162 (58.64%), Query Frame = 0


Query: 1   MSLDALSSNDFFNFIIYDTISAIPNTSPI-LHDSSEN-TFFSDDTCSRHQDHQHGLEGSL 60
           M+L+ALSSN   NF++ +T+S  P  S + L    EN    S +T S   + +       
Sbjct: 1   MTLEALSSNGLLNFLLSETLSPTPFKSLVDLEPLPENDVIISKNTISEISNQE------- 60

Query: 61  KPDRKCRPSESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEH 120
            P ++  P         +T +GKK+R+RKPRVCKN+EEAE QRMTHIAVERNRR+QMN+H
Sbjct: 61  PPPQRQPP---------ATNRGKKRRRRKPRVCKNEEEAENQRMTHIAVERNRRRQMNQH 120

Query: 121 LSVLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEE 161
           LSVLRSLMP+ +  + ++   +    D      H +  +E +
Sbjct: 121 LSVLRSLMPQPFAHKGDQASIVGGAIDFIKELEHKLLSLEAQ 146

[Close]

BLAST of Carg24527 vs. TAIR 10
Match: AT1G22490.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 9.1e-15
Identity = 41/77 (53.25%), Postives = 56/77 (72.73%), Query Frame = 0


Query: 82  KKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLSVLRSLMPESYVQRVEEGCQLR 141
           ++++R+ R CKNKEE E QRMTHIAVERNRRKQMNE+L+VLRSLMP SY QR ++   + 
Sbjct: 95  RRKRRRTRNCKNKEEIENQRMTHIAVERNRRKQMNEYLAVLRSLMPSSYAQRGDQASIVG 154

Query: 142 SVEDIAAAAHHMVRIIE 159
              +      H+++ +E
Sbjct: 155 GAINYVKELEHILQSME 171

[Close]

BLAST of Carg24527 vs. TAIR 10
Match: AT3G24140.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.2e-13
Identity = 44/93 (47.31%), Postives = 58/93 (62.37%), Query Frame = 0


Query: 68  ESESLRTASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLSVLRSLMP 127
           E    +  + ++ K KRKR  R  K  EE E+QRMTHIAVERNRRKQMNEHL VLRSLMP
Sbjct: 164 EDRENKNVTKKEVKSKRKR-ARTSKTSEEVESQRMTHIAVERNRRKQMNEHLRVLRSLMP 223

Query: 128 ESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEE 161
            SYVQR ++   +    +       +++ +E +
Sbjct: 224 GSYVQRGDQASIIGGAIEFVRELEQLLQCLESQ 255

[Close]

BLAST of Carg24527 vs. TAIR 10
Match: AT1G72210.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 8.5e-13
Identity = 38/57 (66.67%), Postives = 47/57 (82.46%), Query Frame = 0


Query: 80  GKKKRKRKPRVCKNKEEAETQRMTHIAVERNRRKQMNEHLSVLRSLMPESYVQRVEE 137
           G ++++R+ R  KNKEE E QRMTHIAVERNRRKQMNE+L+VLRSLMP  Y QR ++
Sbjct: 103 GGRRKRRRTRSSKNKEEIENQRMTHIAVERNRRKQMNEYLAVLRSLMPPYYAQRGDQ 159

[Close]

BLAST of Carg24527 vs. TAIR 10
Match: AT4G01460.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 8.5e-13
Identity = 50/110 (45.45%), Postives = 67/110 (60.91%), Query Frame = 0


Query: 54  LEGSLKPDRKCRPSESESLRT---ASTRQGKKKRKRKPRVCKNKEEAETQRMTHIAVERN 113
           LE ++K D        +  RT   A T + K+KRKR  R  KNK+E E QRMTHIAVERN
Sbjct: 65  LETNIKRDPGQTDDPEKDPRTENGAVTVKEKRKRKR-TRAPKNKDEVENQRMTHIAVERN 124

Query: 114 RRKQMNEHLSVLRSLMPESYVQRVEEGCQLRSVEDIAAAAHHMVRIIEEE 161
           RR+QMNEHL+ LRSLMP S++QR ++   +    D       +++ +E E
Sbjct: 125 RRRQMNEHLNSLRSLMPPSFLQRGDQASIVGGAIDFIKELEQLLQSLEAE 173

The following BLAST results are available for this feature:

BLAST of Carg24527 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011385.1	2.8e-82	100.00	Transcription factor bHLH71, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
KAG6571656.1	9.9e-64	82.10	Transcription factor basic helix-loop-helix 94, partial [Cucurbita argyrosperma ...	[more]
XP_023554782.1	3.8e-63	81.48	transcription factor bHLH71-like [Cucurbita pepo subsp. pepo]	[more]
XP_022963757.1	4.9e-63	81.48	transcription factor bHLH71-like [Cucurbita moschata]	[more]
XP_022967282.1	9.6e-59	77.78	transcription factor bHLH71-like [Cucurbita maxima]	[more]

BLAST of Carg24527 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q56XR0	1.6e-19	41.36	Transcription factor bHLH71 OS=Arabidopsis thaliana OX=3702 GN=BHLH71 PE=1 SV=1	[more]
Q9SK91	1.3e-13	53.25	Transcription factor bHLH94 OS=Arabidopsis thaliana OX=3702 GN=BHLH94 PE=1 SV=2	[more]
Q56YJ8	3.1e-12	47.31	Transcription factor FAMA OS=Arabidopsis thaliana OX=3702 GN=FAMA PE=1 SV=1	[more]
Q9M128	1.2e-11	45.45	Transcription factor bHLH57 OS=Arabidopsis thaliana OX=3702 GN=BHLH57 PE=1 SV=1	[more]
Q9C7T4	1.2e-11	66.67	Transcription factor bHLH96 OS=Arabidopsis thaliana OX=3702 GN=BHLH96 PE=1 SV=1	[more]

BLAST of Carg24527 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HG33	2.4e-63	81.48	transcription factor bHLH71-like OS=Cucurbita moschata OX=3662 GN=LOC111463954 P...	[more]
A0A6J1HQE1	4.6e-59	77.78	transcription factor bHLH71-like OS=Cucurbita maxima OX=3661 GN=LOC111466851 PE=...	[more]
A0A6J1ESZ0	8.5e-37	59.52	transcription factor bHLH71-like OS=Cucurbita moschata OX=3662 GN=LOC111437434 P...	[more]
A0A6J1KA87	1.6e-35	58.33	transcription factor bHLH71-like OS=Cucurbita maxima OX=3661 GN=LOC111491514 PE=...	[more]
A0A0A0LF14	1.6e-35	58.08	BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G901180 PE=4 S...	[more]

BLAST of Carg24527 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46690.1	1.1e-20	41.36	beta HLH protein 71	[more]
AT1G22490.1	9.1e-15	53.25	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT3G24140.1	2.2e-13	47.31	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G72210.1	8.5e-13	66.67	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT4G01460.1	8.5e-13	45.45	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 100..127 e-value: 9.7E-8 score: 31.9
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 99..157 score: 10.829479
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 91..161 e-value: 5.3E-8 score: 34.6
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 92..147
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..99
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..69
None	No IPR available	PANTHER	PTHR11969	MAX DIMERIZATION, MAD	coord: 133..163
None	No IPR available	PANTHER	PTHR11969:SF54	TRANSCRIPTION FACTOR BHLH71	coord: 133..163
None	No IPR available	PANTHER	PTHR11969	MAX DIMERIZATION, MAD	coord: 1..133
None	No IPR available	PANTHER	PTHR11969:SF54	TRANSCRIPTION FACTOR BHLH71	coord: 1..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24527-RA	Carg24527-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046983	protein dimerization activity