Carg24434 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24434
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionIAA-amino acid hydrolase ILR1-like 6
LocationCarg_Chr04: 18420585 .. 18425184 (+)
RNA-Seq ExpressionCarg24434
SyntenyCarg24434
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCTCGCTAAACTTCTGCATAATTTTTACTATCGTGATTCAATGCTTCTCAATCTCCTTCATCGACGCCATATCCTCTCCGGTCGGCGGTGATCATGACGGTTCTGCTCTAATAGGCGCAGGCAGCGGCATAAATTCTGTGCCGGAGTCTAATTCTCTCAAGAATCAGAGTGTTAGAACGTTGATGGCTCGATCGGACTCTCAGAGCTGCGAGGTGTGGACGGAGGCATGCTCTGAGGCGATTCTCAGTTTGGCGAAGCGGCCGGATGTTGTTGATTGGTTGAAGAAGGTGAGGAGGAGAATCCATGAGAATCCGGAGCTTGCGTTTGAGGAATTTGAGACGAGTCAATTGGTACGCGATGAGTTGGACCGGATGGATGCTAGTTACGAGCATCCGTTTGCTAAGACTGGCGTTCGTGCTTGGATCGGGACTGGCGGACCGCCTTTTGTTGCTCTTAGGGCAGATATGGACGCTCTTCCAATCCAGGTTCTTCTAGAAAATCGGATATCGCGAATATGATTTTGATTTCTGTTCTTTCTTTTTTCCTCTGGCTTTCGCATTTACGATTGATTGATTGCCTTGATGTTTCTGTTCTGCCTTGAGATTGAAAACTACGAGCGAAAAAAAGAGAGAGAGGAAGAACACCAAGATTTAGAATTAAAAAAACTCTGCTCTAAAAGCAATTTCTGCAAATGCGTTCGAGCAATGGATTTTTTTCTTCTTTTGAAATCAGTTGGAATTTGTTTTCAAATATTCCATTACTGAACTTCCACGATCTCACTTGTCACTTTTACAGCTCCTTACGCACAGCTATCTTTTCAGAAATGTAGAAATGTATATCATGTTTTAACTTCTACTCTAATTTTTGGATCGTCATAAAATTATATCGTCAAATGTTTCTTCCTCAAATTTCTTTTACACTGAAATTAGATTTTAATAACCAAGCATCATTCTCGAAGAACTCGAAACGTTTTTATCAATTTGGCATCATTGTGTCAGGGCATTAACAATAGTGGTCCATCTCTATTTCCCTCTCTATTACTTGTCACGTGTTTGAGTTCCCTCCTTCAAATAAATAAATTAATCTAACTTACAGGAGAAAGAGGAAGCGGTCTAGATGACATGGTTTACAAATACGATATTTTACGTTTTCCTTAGAAAAAGTCAGTACAATTCTTAGCCCCATAATTTTTCGTGGATAAAGGATAAGTGCAAGTGGTTGGCGTGGTGTCTGTTTGTACCCCCTCACAGGTTGAGGTGGGACAATACTTTTTAATTCTTTAGGTATACTTGACATTTTAATTGTATTTGAGTAATACAAAAATCAACTAACAATTGGTGGAACTCAGTCCTACACGCTAAACATCTTGACTTTAAATTTTATTTTTATCCTTCAGCTTACTTTAGGATGATTTTTTCATTTGGAGTGTTTGTATTTTTCATTTAGTTGAAATCAATCATTCACGTTAAACATTGATTTTTTTTTTTTTTTTTTTTTTTTTTTGAACTTTGATGGCGATTTTAGGATTATGCTTAAATATGGAGTGTTTAATTTTACTTTTCTAATTTTCTGGGGTTGATCTTTCGTGAAGCCGATTGGTTTCAATATTACTTATTGATACTATTACTTATGAAATCGGAAAATGTAAAATGGCATAAAACACTATGCAGGAAGCCGTGGAATGGGAGCACAAAAGCAGAGTACCAGGCAAGATGCATGCTTGTGGGCACGATGCTCACGTTACAATGCTTCTTGGTGCTGCCAAGATCTTGAAGGATCGTGAACATCTCTTGAAGGTCTGCCTAACACCCTCAACTTATTTTGATTTTCTGGAAACAAATAATTTTAAAAGAGTTAATACTATAAGGTTCTCTGATTCCAAGCCTCCAAGATGATGGAGTTTTTTCTTTTTCTTTTAGGAGATGAAATTAAAGAAACGCTAGCGTTAAAATAATGGGTAAATTATTTTGCAAAAATAAAGATGGACAAATTAGGCAAATTTACTTTCATAGTTGCAGAAATTTATAATATAGACTACTTATATTGTCACTGACCGTAATAAATATAAACATTTATGATACAGGCCACCATAGTTGTCATCGACCATAATAAATATAAATAGACCACTATAATTTTAATTATCAATGTCCATAATAAATACATTTAATTAATTGCTCTCGAAGTCTTCTCGCCACAGGTAGAATGTTTTATATGGACAAGTGGCTTGTCACGGAAGACTTTTTAACCTTACCAACCAAAACTATAGTTTGGTGGTTGAAATTAGCAAAAATTACAATTACTATTTACTCACTATTTACACGCTCATAACTTAGATGCAAGATGTCATCAACGGTAAAAGTAACATACTGAGCATAATTAAAATACATTTAGAGTACTCTTCTGTTAAGTTTTTTTAATATGATACTCCGACCATTATTCCCAACCACTTTACCAAAATGAAATAAAGAAAGAGAACAAAACAAGGATGGTTCTGACACTCTTCAAACTAGTAGACCAGCTGGCGACATGATTTTAGATTCTTTGTTGGGTTGCTGCGATGTTTCTCGAGTTTATCTCATATTTGTATTCTTTTATCTGTGTGGAGTACTAAAATCCGTGGCTACCCATCACTCATTTTCAGTTCTAAGTACTGTGTTTTACAGGGAACAGTGGTTTTGCTGTTTCAGCCAGCTGAAGAAGCTGGCAATGGCGCAAAGCGAATGATTGGCGATGGAGCTTTAAGAAACGTTCAAGCCATTTTTGCTGTTCATGTTTCTCATGAACTTCCCACCGCCATTATTGGGTCCCGGCCTGGTCCTCTGCTAGCTGGCTGTGGCTTCTTTAGGGCGGTGATTACCGGCAAAAAGGACCAGGCTGGGTCGCCTCACCGGTCCGTCGACCCGGTTCTGGCTGCCTCGGCCGCCGTTATCAGTCTGCAGGGGATTGTGTCTCGTGAAGCAAATCCTTTAGATTCGCAGGTGCGGTTTTTTTTTTTTTGGGGGGGGGGTTTGTTTGTTTTTGATTTAGGGACTTTTTCAATACTGAATTATCAATTCGTTGCCCTTATGATTTTTGTTTTTGTACTCAGGTTGTGTCAGTGACTTGGTTCAATGGAGGGAGTAACCTGGATGTGATACCAGACACTGTGGTCATTGGCGGTACTTTCAGGGCTTTCTCCAACTCTAGCTTCTACCAACTTCTTCAAAGAATAGAGGAGGTAATTTCTCCCTTCACTTTCAAGCCCCACCGTTCCATTTTTCAGCTTCAAACTCACCTTAAATGTTTCTTAAAAGATTAAAGAATTATCTTTGTTAACTAAATGCTTTTTTCAAAGTTTTTTTTTTTTTTTTTTCAGAGGTAATTGATGGGGAAAATTCTAAAAAGTTCTTAAAAAAACTGAAATCCCAAGAATGAATTAGGAATGACTTCAGCCCATTGAGCACTTCTTGAAAGAAAATTCAATGTACGGTTGCTTTAGATTAGATTATTGATCAAAATGATTTTGGAAATTCTAAAACCAAAAATTGCTTTGAGGAGAAGCGTTAAATTAGTGCATTTCTCCAAAAGCACTTCTAATTGGGCAGCCTTTTTTTTTTTTCTCTCTCCTGAAAATTTATCTCAATATCACATTTTAAAGTCGTATATCTAATCTCACCGCTATTAAATATTGTGTTGTTTGAGCTTCTTCTCAGTTTTTAAAATGTCCTCCTATTAGAGAGAAGTTTTAACACCCTTCTAAAGAATGTTTTGTTTCTTCTCCAACCGATGTGGGTTCTCACAATTCACTCTCTTTGGGATCCAACATCTTCGTGACACTCGTTCCTCTCTCTAATCGATATGACATCTCACAATCTACTCCCCTTCGAGATCTAGCATTCTCGCTCGTACACCGCTCGGTGTCCACCCTCTTCAAGATCTAGTGTTCTCGCTCGTACACCGCCTAGTGTCTACCCCCTTCGTCTCAGTGTCCTAAGTTAGCACACCGCTCGATGTCTGGCTCTGATACCATTTGTAACAGCCCAAACTCACCGCTAGTAGATATTATCCTCTTTGAGTTTTCCTTACGGCTTTTCCTCATAATTTTTAAAACGCGTTTGCTAGGATGAAGTTTCTACATCTCTATAAAGAATGCTTCGTTTCCCCTCCCCTTTCCCACAAACGTGGCATCTCGTGTACCAAAATTACCTTTAGTTAAATTTGGCTCATAAACGATCTACACTTCAGGTAATTGTGGAGCAGGCCAGAGTTTATAGATGCTCGACAACGGTGGACTTCTTTGAAAAAGAATACACCATATACCCTCCAACCGTGAACGACGAAAAGATGTATGAGCATGTGAAGAAAGTGGCCACCGACCTACATGGCTCACACAATTACAGGGTGGTCCCACCAATGATGGGGGCCGAAGATTTTTCCTTTTACTCGGAGTTTGTTCCTACAGCGTACTATTACATAGGAGTCAGGAACGAGACCTTAGGCTCCATCCACACAGGCCACTCTCCCTATTTCATGATCGACGAAGACATGCTACCCATTGGCGCTGCCACTCATGCAACCATCGCCGAGAGGTATCTGTACGAGCATGGAGAATGA

mRNA sequence

ATGGATTCCTCGCTAAACTTCTGCATAATTTTTACTATCGTGATTCAATGCTTCTCAATCTCCTTCATCGACGCCATATCCTCTCCGGTCGGCGGTGATCATGACGGTTCTGCTCTAATAGGCGCAGGCAGCGGCATAAATTCTGTGCCGGAGTCTAATTCTCTCAAGAATCAGAGTGTTAGAACGTTGATGGCTCGATCGGACTCTCAGAGCTGCGAGGTGTGGACGGAGGCATGCTCTGAGGCGATTCTCAGTTTGGCGAAGCGGCCGGATGTTGTTGATTGGTTGAAGAAGGTGAGGAGGAGAATCCATGAGAATCCGGAGCTTGCGTTTGAGGAATTTGAGACGAGTCAATTGGTACGCGATGAGTTGGACCGGATGGATGCTAGTTACGAGCATCCGTTTGCTAAGACTGGCGTTCGTGCTTGGATCGGGACTGGCGGACCGCCTTTTGTTGCTCTTAGGGCAGATATGGACGCTCTTCCAATCCAGGAAGCCGTGGAATGGGAGCACAAAAGCAGAGTACCAGGCAAGATGCATGCTTGTGGGCACGATGCTCACGTTACAATGCTTCTTGGTGCTGCCAAGATCTTGAAGGATCGTGAACATCTCTTGAAGGGAACAGTGGTTTTGCTGTTTCAGCCAGCTGAAGAAGCTGGCAATGGCGCAAAGCGAATGATTGGCGATGGAGCTTTAAGAAACGTTCAAGCCATTTTTGCTGTTCATGTTTCTCATGAACTTCCCACCGCCATTATTGGGTCCCGGCCTGGTCCTCTGCTAGCTGGCTGTGGCTTCTTTAGGGCGGTGATTACCGGCAAAAAGGACCAGGCTGGGTCGCCTCACCGGTCCGTCGACCCGGTTCTGGCTGCCTCGGCCGCCGTTATCAGTCTGCAGGGGATTGTGTCTCGTGAAGCAAATCCTTTAGATTCGCAGGTTGTGTCAGTGACTTGGTTCAATGGAGGGAGTAACCTGGATGTGATACCAGACACTGTGGTCATTGGCGGTACTTTCAGGGCTTTCTCCAACTCTAGCTTCTACCAACTTCTTCAAAGAATAGAGGAGGTAATTGTGGAGCAGGCCAGAGTTTATAGATGCTCGACAACGGTGGACTTCTTTGAAAAAGAATACACCATATACCCTCCAACCGTGAACGACGAAAAGATGTATGAGCATGTGAAGAAAGTGGCCACCGACCTACATGGCTCACACAATTACAGGGTGGTCCCACCAATGATGGGGGCCGAAGATTTTTCCTTTTACTCGGAGTTTGTTCCTACAGCGTACTATTACATAGGAGTCAGGAACGAGACCTTAGGCTCCATCCACACAGGCCACTCTCCCTATTTCATGATCGACGAAGACATGCTACCCATTGGCGCTGCCACTCATGCAACCATCGCCGAGAGGTATCTGTACGAGCATGGAGAATGA

Coding sequence (CDS)

ATGGATTCCTCGCTAAACTTCTGCATAATTTTTACTATCGTGATTCAATGCTTCTCAATCTCCTTCATCGACGCCATATCCTCTCCGGTCGGCGGTGATCATGACGGTTCTGCTCTAATAGGCGCAGGCAGCGGCATAAATTCTGTGCCGGAGTCTAATTCTCTCAAGAATCAGAGTGTTAGAACGTTGATGGCTCGATCGGACTCTCAGAGCTGCGAGGTGTGGACGGAGGCATGCTCTGAGGCGATTCTCAGTTTGGCGAAGCGGCCGGATGTTGTTGATTGGTTGAAGAAGGTGAGGAGGAGAATCCATGAGAATCCGGAGCTTGCGTTTGAGGAATTTGAGACGAGTCAATTGGTACGCGATGAGTTGGACCGGATGGATGCTAGTTACGAGCATCCGTTTGCTAAGACTGGCGTTCGTGCTTGGATCGGGACTGGCGGACCGCCTTTTGTTGCTCTTAGGGCAGATATGGACGCTCTTCCAATCCAGGAAGCCGTGGAATGGGAGCACAAAAGCAGAGTACCAGGCAAGATGCATGCTTGTGGGCACGATGCTCACGTTACAATGCTTCTTGGTGCTGCCAAGATCTTGAAGGATCGTGAACATCTCTTGAAGGGAACAGTGGTTTTGCTGTTTCAGCCAGCTGAAGAAGCTGGCAATGGCGCAAAGCGAATGATTGGCGATGGAGCTTTAAGAAACGTTCAAGCCATTTTTGCTGTTCATGTTTCTCATGAACTTCCCACCGCCATTATTGGGTCCCGGCCTGGTCCTCTGCTAGCTGGCTGTGGCTTCTTTAGGGCGGTGATTACCGGCAAAAAGGACCAGGCTGGGTCGCCTCACCGGTCCGTCGACCCGGTTCTGGCTGCCTCGGCCGCCGTTATCAGTCTGCAGGGGATTGTGTCTCGTGAAGCAAATCCTTTAGATTCGCAGGTTGTGTCAGTGACTTGGTTCAATGGAGGGAGTAACCTGGATGTGATACCAGACACTGTGGTCATTGGCGGTACTTTCAGGGCTTTCTCCAACTCTAGCTTCTACCAACTTCTTCAAAGAATAGAGGAGGTAATTGTGGAGCAGGCCAGAGTTTATAGATGCTCGACAACGGTGGACTTCTTTGAAAAAGAATACACCATATACCCTCCAACCGTGAACGACGAAAAGATGTATGAGCATGTGAAGAAAGTGGCCACCGACCTACATGGCTCACACAATTACAGGGTGGTCCCACCAATGATGGGGGCCGAAGATTTTTCCTTTTACTCGGAGTTTGTTCCTACAGCGTACTATTACATAGGAGTCAGGAACGAGACCTTAGGCTCCATCCACACAGGCCACTCTCCCTATTTCATGATCGACGAAGACATGCTACCCATTGGCGCTGCCACTCATGCAACCATCGCCGAGAGGTATCTGTACGAGCATGGAGAATGA

Protein sequence

MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSVRTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFYSEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE
Homology
BLAST of Carg24434 vs. NCBI nr
Match: KAG7032805.1 (IAA-amino acid hydrolase ILR1-like 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 958.0 bits (2475), Expect = 3.1e-275
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV
Sbjct: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
           RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
           RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 476

BLAST of Carg24434 vs. NCBI nr
Match: XP_022955832.1 (IAA-amino acid hydrolase ILR1-like 6 [Cucurbita moschata])

HSP 1 Score: 955.3 bits (2468), Expect = 2.0e-274
Identity = 475/476 (99.79%), Postives = 475/476 (99.79%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV
Sbjct: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
           RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKK QAGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKGQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
           RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 476

BLAST of Carg24434 vs. NCBI nr
Match: KAG6602101.1 (IAA-amino acid hydrolase ILR1-like 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 951.0 bits (2457), Expect = 3.8e-273
Identity = 473/476 (99.37%), Postives = 474/476 (99.58%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV
Sbjct: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
           RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGAL NVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALINVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKK QAGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKYQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
           RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLP+GAATHATIAERYLYEHGE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPVGAATHATIAERYLYEHGE 476

BLAST of Carg24434 vs. NCBI nr
Match: XP_023533052.1 (IAA-amino acid hydrolase ILR1-like 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 945.7 bits (2443), Expect = 1.6e-271
Identity = 470/476 (98.74%), Postives = 472/476 (99.16%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           MDSSLNFCIIFTIVIQCFSI FIDAISSPVGGDHDGSALIGAGSGINSVP+SNSLKNQSV
Sbjct: 1   MDSSLNFCIIFTIVIQCFSICFIDAISSPVGGDHDGSALIGAGSGINSVPDSNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
           RTLMARSDSQ CEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQRCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPG MH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGNMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKK QAGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKAQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
           RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYE+GE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEYGE 476

BLAST of Carg24434 vs. NCBI nr
Match: XP_022990038.1 (IAA-amino acid hydrolase ILR1-like 6 [Cucurbita maxima])

HSP 1 Score: 938.3 bits (2424), Expect = 2.5e-269
Identity = 468/476 (98.32%), Postives = 470/476 (98.74%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           MDSSLNFCIIFTIV QCFSISFID ISSPVGGDHDGSALIGA SGINSVP+SNSLKNQSV
Sbjct: 1   MDSSLNFCIIFTIV-QCFSISFIDTISSPVGGDHDGSALIGADSGINSVPDSNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
           RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAA ILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAMILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKK QAGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKGQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVTWFNGGS LDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSKLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
           RVYRCSTTVDFFEKEYTIYPPT+NDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTMNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 475

BLAST of Carg24434 vs. ExPASy Swiss-Prot
Match: Q8VYX0 (IAA-amino acid hydrolase ILR1-like 6 OS=Arabidopsis thaliana OX=3702 GN=ILL6 PE=1 SV=2)

HSP 1 Score: 590.5 bits (1521), Expect = 1.7e-167
Identity = 292/417 (70.02%), Postives = 341/417 (81.77%), Query Frame = 0

Query: 55  LKNQSVRTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEF 114
           +KNQS+  L A   S  C VWT+ACS+ IL L  +PD V WLK+VRR IHENPELAFEE+
Sbjct: 49  IKNQSL-GLPAHVGSDECRVWTKACSDEILRLTYQPDNVAWLKRVRRTIHENPELAFEEY 108

Query: 115 ETSQLVRDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSR 174
           ETS+L+R ELDRM   Y +P AKTG+RAWIG+GGPPFVA+RADMDALPIQEAVEWEH S+
Sbjct: 109 ETSRLIRSELDRMGIMYRYPLAKTGIRAWIGSGGPPFVAVRADMDALPIQEAVEWEHISK 168

Query: 175 VPGKMHACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRN 234
           V GKMHACGHDAHVTMLLGAA ILK REHLLKGTVVLLFQPAEEAGNGAK MI DGAL +
Sbjct: 169 VAGKMHACGHDAHVTMLLGAAHILKAREHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDD 228

Query: 235 VQAIFAVHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAV 294
           V+AIFAVHVSH  PT +IGSR GPLLAGCG FRAVIT  +D  G    + + +LAAS+AV
Sbjct: 229 VEAIFAVHVSHIHPTGVIGSRSGPLLAGCGIFRAVIT-SEDSRG----AANLLLAASSAV 288

Query: 295 ISLQGIVSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEE 354
           ISLQGIVSREA+PLDSQVVSVT F+GG +LDV PDTVV+GGTFRAFSNSSFY L +RI+E
Sbjct: 289 ISLQGIVSREASPLDSQVVSVTSFDGGHSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQE 348

Query: 355 VIVEQARVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGA 414
           V+++Q  V+ C  TV+FFEK+  IYPPT N++  Y H+KKV  DL G  ++ + P MMGA
Sbjct: 349 VLMDQVGVFGCQATVNFFEKQNAIYPPTTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGA 408

Query: 415 EDFSFYSEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYL 472
           EDF+FYSE +P A+Y+IG+RNE LGS+H  HSP+FMIDED LP+GAA HA +AERYL
Sbjct: 409 EDFAFYSEIIPAAFYFIGIRNEELGSVHIAHSPHFMIDEDSLPVGAAVHAAVAERYL 459

BLAST of Carg24434 vs. ExPASy Swiss-Prot
Match: Q5Z678 (IAA-amino acid hydrolase ILR1-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL6 PE=2 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.3e-151
Identity = 275/402 (68.41%), Postives = 313/402 (77.86%), Query Frame = 0

Query: 81  EAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPFAKTGV 140
           E I  +A RP+   WL+ VRRRIHE PELA+EE ETS+LVRDELD M   + HP A+TGV
Sbjct: 97  EEIAGMAGRPETAAWLRAVRRRIHERPELAYEEVETSRLVRDELDAMGVGFRHPVARTGV 156

Query: 141 RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAAKILKD 200
            A IGTG PP VALRADMDALPIQEAVEWEHKS+ PGKMHACGHDAHV MLLGAAKILK 
Sbjct: 157 VANIGTGRPPVVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDAHVAMLLGAAKILKA 216

Query: 201 REHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSRPGPLL 260
           REH L+GTV LLFQPAEE+G GAKRMI  GAL +V+AIFAVHVSH+ PT++IGSR GPLL
Sbjct: 217 REHHLRGTVRLLFQPAEESGAGAKRMIEGGALEDVEAIFAVHVSHQHPTSVIGSRTGPLL 276

Query: 261 AGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSVTWFNG 320
           AGCGFF+AVI G +       RS D VLAA++ +ISLQ IVSREA+PLDSQVVSV   NG
Sbjct: 277 AGCGFFKAVIHGGR-------RSGDAVLAAASTIISLQSIVSREADPLDSQVVSVAMVNG 336

Query: 321 GSNLDVI--------PDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFF 380
             +             +  V+GGTFRAFSN+SFYQ+ +RIEEVI  QARV+ C   VDFF
Sbjct: 337 SDHPAATARAAAAEEEEEFVLGGTFRAFSNASFYQVRRRIEEVITAQARVHGCEAAVDFF 396

Query: 381 EKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFYSEFVPTAYYYIG 440
           E + + YPPTVND +MY HVK VA +L G+ +YR VPPMMGAEDFSFYS+ VP  +YYIG
Sbjct: 397 ENQ-SFYPPTVNDARMYAHVKAVAGELLGAGSYRDVPPMMGAEDFSFYSQVVPAGFYYIG 456

Query: 441 VRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEH 475
           VRNETLGS+HTGHSPYFMIDED+LP GAA HA IAERYL  H
Sbjct: 457 VRNETLGSVHTGHSPYFMIDEDVLPTGAAFHAAIAERYLANH 490

BLAST of Carg24434 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.1e-137
Identity = 236/393 (60.05%), Postives = 294/393 (74.81%), Query Frame = 0

Query: 83  ILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPFAKTGVRA 142
           +L  AK  +   W+  +RRRIHENPEL +EEF TS+LVR ELD +   Y HPFA TGV A
Sbjct: 36  LLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVA 95

Query: 143 WIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAAKILKDRE 202
            +GTGGPPFVALRADMDALP+QE+VEWEHKS+VPGKMH CGHDAHV MLLG+A+IL++  
Sbjct: 96  TVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHR 155

Query: 203 HLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSRPGPLLAG 262
             LKGTVVL+FQPAEE G GAK+MI DGA+ N++AIF VHV+  +P  ++ SRPGP++AG
Sbjct: 156 DELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPVMAG 215

Query: 263 CGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSVTWFNGGS 322
            GFF AVI+GK   A  PH ++DP+LAAS  ++SLQ +VSREA+PLDSQVV+V  F GG 
Sbjct: 216 SGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGG 275

Query: 323 NLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFFEKEYTIYPPT 382
             +VIPD+V IGGTFRAF   SF QL QRIEEVIV QA V RC+  VDF +K+   +PPT
Sbjct: 276 AFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPT 335

Query: 383 VNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFYSEFVPTAYYY-IGVRNETLGSI 442
           +N   +++   KVA+++ G  N R   P+MGAEDF+FY++ +P  YYY +G+ NET G  
Sbjct: 336 INSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQ 395

Query: 443 HTGHSPYFMIDEDMLPIGAATHATIAERYLYEH 475
              HSPYF I+ED LP GAA  A++A RYL EH
Sbjct: 396 APHHSPYFTINEDALPYGAALQASLAARYLLEH 428

BLAST of Carg24434 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 1.2e-136
Identity = 235/393 (59.80%), Postives = 293/393 (74.55%), Query Frame = 0

Query: 83  ILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPFAKTGVRA 142
           +L  AK  +   W+  +RRRIHENPEL +EEF TS+LVR ELD +   Y HPFA TGV A
Sbjct: 36  LLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVA 95

Query: 143 WIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAAKILKDRE 202
            +GTGGPPFVALRADMDALP+QE+VEWEHKS+VPGKMH CGHDAHV MLLG+A+IL++  
Sbjct: 96  TVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHR 155

Query: 203 HLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSRPGPLLAG 262
             LKGTVVL+FQPAEE G GAK+MI DG + N++AIF VHV+  +P  ++ SRPGP++AG
Sbjct: 156 DELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPVMAG 215

Query: 263 CGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSVTWFNGGS 322
            GFF AVI+GK   A  PH ++DP+LAAS  ++SLQ +VSREA+PLDSQVV+V  F GG 
Sbjct: 216 SGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGG 275

Query: 323 NLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFFEKEYTIYPPT 382
             +VIPD+V IGGTFRAF   SF QL QRIEEVIV QA V RC+  VDF +K+   +PPT
Sbjct: 276 AFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPT 335

Query: 383 VNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFYSEFVPTAYYY-IGVRNETLGSI 442
           +N   +++   KVA+++ G  N R   P+MGAEDF+FY++ +P  YYY +G+ NET G  
Sbjct: 336 INSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQ 395

Query: 443 HTGHSPYFMIDEDMLPIGAATHATIAERYLYEH 475
              HSPYF I+ED LP GAA  A++A RYL EH
Sbjct: 396 APHHSPYFTINEDALPYGAALQASLATRYLLEH 428

BLAST of Carg24434 vs. ExPASy Swiss-Prot
Match: Q5N8F2 (IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL2 PE=2 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.6e-133
Identity = 235/394 (59.64%), Postives = 292/394 (74.11%), Query Frame = 0

Query: 83  ILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPFAKTGVRA 142
           +L  A+RP+   W+  VR  IHE PELAFEE ETS+LVR ELD M  +Y HP A TGV A
Sbjct: 50  VLDRARRPEFAAWMAGVRWAIHERPELAFEEIETSRLVRAELDAMGVAYRHPVAGTGVVA 109

Query: 143 WIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAAKILKDRE 202
            +GTG PPFVALRADMDALP+QE V+WEHKS+V  KMHACGHDAH TMLLGAA+IL++R 
Sbjct: 110 TVGTGRPPFVALRADMDALPMQEEVQWEHKSKVAMKMHACGHDAHTTMLLGAARILQERR 169

Query: 203 HLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSRPGPLLAG 262
           H L+GTVVLLFQP EE G GA+RM+  GA+ NV+AIF  HVS ELPT ++GSRPGPLLAG
Sbjct: 170 HELQGTVVLLFQPGEEVGTGARRMVEAGAVDNVEAIFGFHVSVELPTGVVGSRPGPLLAG 229

Query: 263 CGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSVTWFNGGS 322
           CGFF AVITGK   A  PH SVDP+LAAS  V++LQG+VSREA+PL++QVV+VT F  G 
Sbjct: 230 CGFFEAVITGKGGHAAHPHASVDPILAASTVVLALQGLVSREADPLEAQVVTVTRFLAGD 289

Query: 323 NLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFFEKEYTIYPPT 382
            L+VIP+++ IGGTFR FSN  F +L +RIEEVIV Q+ VYRC+  VDF      + PPT
Sbjct: 290 ALNVIPESITIGGTFRVFSNEGFLRLKRRIEEVIVAQSAVYRCAAAVDFHAGGRPLLPPT 349

Query: 383 VNDEKMYEHVKKVATDLHGSHN--YRVVPPMMGAEDFSFYSEFVPTA-YYYIGVRNETLG 442
           +N   ++ H + VA +  G+       + P MG+EDF+ +SE VP + +Y++GVRNE  G
Sbjct: 350 INSAALHAHFQAVAAETLGASAAVLGAMEPCMGSEDFAVFSEAVPASHFYFVGVRNEAEG 409

Query: 443 SIHTGHSPYFMIDEDMLPIGAATHATIAERYLYE 474
            +H  HSP+F +D+  LP GAA HA++A RYL E
Sbjct: 410 LVHLAHSPHFRVDDAALPYGAALHASLAMRYLDE 443

BLAST of Carg24434 vs. ExPASy TrEMBL
Match: A0A6J1GUP6 (IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita moschata OX=3662 GN=LOC111457704 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 9.7e-275
Identity = 475/476 (99.79%), Postives = 475/476 (99.79%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV
Sbjct: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
           RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKK QAGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKGQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
           RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 476

BLAST of Carg24434 vs. ExPASy TrEMBL
Match: A0A6J1JS37 (IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita maxima OX=3661 GN=LOC111487050 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.2e-269
Identity = 468/476 (98.32%), Postives = 470/476 (98.74%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           MDSSLNFCIIFTIV QCFSISFID ISSPVGGDHDGSALIGA SGINSVP+SNSLKNQSV
Sbjct: 1   MDSSLNFCIIFTIV-QCFSISFIDTISSPVGGDHDGSALIGADSGINSVPDSNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
           RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAA ILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAMILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKK QAGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKGQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVTWFNGGS LDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSKLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
           RVYRCSTTVDFFEKEYTIYPPT+NDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTMNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 475

BLAST of Carg24434 vs. ExPASy TrEMBL
Match: A0A6J1JWG3 (IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita maxima OX=3661 GN=LOC111489479 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.3e-239
Identity = 412/476 (86.55%), Postives = 441/476 (92.65%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           M S    C+IF I IQ FS+  IDAI SPVGGDH GS+LIGAG G+ +V  SNSLKNQSV
Sbjct: 1   MFSPARLCLIFFIAIQSFSVCLIDAIFSPVGGDHGGSSLIGAGCGLQTVRNSNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
            TLM R +SQ+CEVWTEACSEAILSLAKRP+VVDWLKKVRR IHENPELAFEEFETSQLV
Sbjct: 61  GTLMTRLESQNCEVWTEACSEAILSLAKRPEVVDWLKKVRRIIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRMD SYE+P A+TG+RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH
Sbjct: 121 RDELDRMDISYEYPLAQTGIRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAAKILKDREHLLKGTV+LLFQPAEEAGNGAKRMIGDGALR+VQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHE PTA IGSRPGPLLAGCGFF+AVITGK  +AGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHEYPTATIGSRPGPLLAGCGFFKAVITGKMGRAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVTWFNGG +LD+IPDTVVIGGTFRA+SNSSFYQ+LQRI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGKDLDMIPDTVVIGGTFRAYSNSSFYQVLQRIKEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
            VYRCS  VDFFE+EYTIYPPTVNDEKMYEHVKKVA DL+GS N+RVV PMMGAEDFSFY
Sbjct: 361 SVYRCSAMVDFFEREYTIYPPTVNDEKMYEHVKKVAIDLYGSPNFRVVRPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           ++ VP A+YY+G+RNETLGSIHTGHSPYFMIDED+LPIGAATHATIAERYLYEHGE
Sbjct: 421 TQLVPAAFYYVGIRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 476

BLAST of Carg24434 vs. ExPASy TrEMBL
Match: A0A6J1FFS9 (IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita moschata OX=3662 GN=LOC111445084 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 3.8e-239
Identity = 413/476 (86.76%), Postives = 440/476 (92.44%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           M SS   C+IF I IQ FS+  IDAI SPVGGDH GS+LIGAG G  +V  SNSLKNQSV
Sbjct: 1   MFSSARLCLIFFIAIQSFSVCLIDAIFSPVGGDHGGSSLIGAGCGFQTVRNSNSLKNQSV 60

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
            TLM R +SQ+CEVWTEACSEAILSLAKRP+VVDWLKKVRR IHENPELAFEEFETSQLV
Sbjct: 61  GTLMTRLESQNCEVWTEACSEAILSLAKRPEVVDWLKKVRRIIHENPELAFEEFETSQLV 120

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           R ELDRMD SYE+P AKTG+RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH
Sbjct: 121 RGELDRMDISYEYPLAKTGIRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAAKILKDREHLLKGTV+LLFQPAEEAGNGAKRMIGDGALR+VQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
           VHVSHE PTA IGSRPGPLLAGCGFF+AVITGK  +AGSPHRSVDPVLAASAAVISLQGI
Sbjct: 241 VHVSHEYPTATIGSRPGPLLAGCGFFKAVITGKMGRAGSPHRSVDPVLAASAAVISLQGI 300

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVT FNGG++LD+IPDTVVIGGTFRA+SNSSFYQ+LQRI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTSFNGGNSLDIIPDTVVIGGTFRAYSNSSFYQVLQRIKEVIVEQA 360

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
            VYRCS  VDFFE+EYTIYPPTVNDEKMYEHVKKVA DLHGS N+RVV PMMGAEDFSFY
Sbjct: 361 SVYRCSAMVDFFEREYTIYPPTVNDEKMYEHVKKVAIDLHGSPNFRVVQPMMGAEDFSFY 420

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           ++ VP A+YY+G+RNETLGSIHTGHSPYFMIDED+LPIGAATHATIAERYLYEHGE
Sbjct: 421 TQLVPAAFYYVGIRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 476

BLAST of Carg24434 vs. ExPASy TrEMBL
Match: A0A0A0KDD2 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G384060 PE=3 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.9e-231
Identity = 405/476 (85.08%), Postives = 431/476 (90.55%), Query Frame = 0

Query: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60
           M SSLNF  IF I I  FS+S     SSP GGDH GS L+GA   +  +  SNSLKNQS+
Sbjct: 87  MISSLNFFSIFIIFIHFFSLS----SSSPAGGDHGGSPLVGAACALQPLRISNSLKNQSI 146

Query: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120
             L+++  SQSCEVWTEACSEAILSLAKRP+VVDWLKKVRRRIHENPELAFEEFETSQL+
Sbjct: 147 GALVSQLASQSCEVWTEACSEAILSLAKRPEVVDWLKKVRRRIHENPELAFEEFETSQLI 206

Query: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180
           RDELDRM+ SYEH  AKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 207 RDELDRMEISYEHMLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVAGKMH 266

Query: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240
           ACGHDAHVTMLLGAAKILK REHLLKGTV+LLFQPAEEAGNGAKRMIGDGALR+VQAIFA
Sbjct: 267 ACGHDAHVTMLLGAAKILKAREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 326

Query: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGI 300
            HVSHE PTA+IGSRPGPLLAGCGFFRAVITGKK  AGSPHRSVDPVLAASAAV+SLQGI
Sbjct: 327 AHVSHEHPTAVIGSRPGPLLAGCGFFRAVITGKKGHAGSPHRSVDPVLAASAAVVSLQGI 386

Query: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360
           VSREANPLDSQVVSVT FNGGSNLD+IPD VVIGGTFRAFSNSSFYQ+LQRIE+VIVEQA
Sbjct: 387 VSREANPLDSQVVSVTSFNGGSNLDMIPDVVVIGGTFRAFSNSSFYQVLQRIEQVIVEQA 446

Query: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420
            VYRCS  VDFFEKEYTIYPPTVND+ MYEHVKKVA DLHGS N+R+V PMMGAEDFSFY
Sbjct: 447 SVYRCSAMVDFFEKEYTIYPPTVNDKAMYEHVKKVAIDLHGSQNFRIVQPMMGAEDFSFY 506

Query: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 477
           SE+VP A++YIGVRNETLGSIHTGHSPYFMIDE++LPIGAATHATIAERYLYEHGE
Sbjct: 507 SEYVPAAFFYIGVRNETLGSIHTGHSPYFMIDENVLPIGAATHATIAERYLYEHGE 558

BLAST of Carg24434 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 590.5 bits (1521), Expect = 1.2e-168
Identity = 292/417 (70.02%), Postives = 341/417 (81.77%), Query Frame = 0

Query: 55  LKNQSVRTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEF 114
           +KNQS+  L A   S  C VWT+ACS+ IL L  +PD V WLK+VRR IHENPELAFEE+
Sbjct: 49  IKNQSL-GLPAHVGSDECRVWTKACSDEILRLTYQPDNVAWLKRVRRTIHENPELAFEEY 108

Query: 115 ETSQLVRDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSR 174
           ETS+L+R ELDRM   Y +P AKTG+RAWIG+GGPPFVA+RADMDALPIQEAVEWEH S+
Sbjct: 109 ETSRLIRSELDRMGIMYRYPLAKTGIRAWIGSGGPPFVAVRADMDALPIQEAVEWEHISK 168

Query: 175 VPGKMHACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRN 234
           V GKMHACGHDAHVTMLLGAA ILK REHLLKGTVVLLFQPAEEAGNGAK MI DGAL +
Sbjct: 169 VAGKMHACGHDAHVTMLLGAAHILKAREHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDD 228

Query: 235 VQAIFAVHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAV 294
           V+AIFAVHVSH  PT +IGSR GPLLAGCG FRAVIT  +D  G    + + +LAAS+AV
Sbjct: 229 VEAIFAVHVSHIHPTGVIGSRSGPLLAGCGIFRAVIT-SEDSRG----AANLLLAASSAV 288

Query: 295 ISLQGIVSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEE 354
           ISLQGIVSREA+PLDSQVVSVT F+GG +LDV PDTVV+GGTFRAFSNSSFY L +RI+E
Sbjct: 289 ISLQGIVSREASPLDSQVVSVTSFDGGHSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQE 348

Query: 355 VIVEQARVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGA 414
           V+++Q  V+ C  TV+FFEK+  IYPPT N++  Y H+KKV  DL G  ++ + P MMGA
Sbjct: 349 VLMDQVGVFGCQATVNFFEKQNAIYPPTTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGA 408

Query: 415 EDFSFYSEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYL 472
           EDF+FYSE +P A+Y+IG+RNE LGS+H  HSP+FMIDED LP+GAA HA +AERYL
Sbjct: 409 EDFAFYSEIIPAAFYFIGIRNEELGSVHIAHSPHFMIDEDSLPVGAAVHAAVAERYL 459

BLAST of Carg24434 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 472.2 bits (1214), Expect = 4.8e-133
Identity = 225/390 (57.69%), Postives = 294/390 (75.38%), Query Frame = 0

Query: 84  LSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPFAKTGVRAW 143
           L+LAKR D  DW+  +RRRIHENPEL +EE ETS+LVR EL++M  SY++P A TGV  +
Sbjct: 36  LTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGY 95

Query: 144 IGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAAKILKDREH 203
           +GTG  PFVALRADMDAL +QE VEWEHKS+VPGKMHACGHDAH TMLLGAAK+LK+ E 
Sbjct: 96  VGTGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEE 155

Query: 204 LLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSRPGPLLAGC 263
            L+GTVVL+FQPAEE G GAK+++  G L NV AIF +HV+++L    + SR GP+LAG 
Sbjct: 156 ELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGS 215

Query: 264 GFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSVTWFNGGSN 323
           GFF+A I+GK   A  P  ++DP+LAAS  ++SLQ +VSREA+PLDSQVV+V  F GG  
Sbjct: 216 GFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGA 275

Query: 324 LDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFFEKEYTIYPPTV 383
            +VIPD+V IGGTFRAFS  SF QL +RIE+VI  QA V  C+ TVDF E+E   +PPTV
Sbjct: 276 FNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTV 335

Query: 384 NDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFYSEFVPTAYYYIGVRNETLGSIHT 443
           ND+ +++  K V+ D+ G  NY  + P+MG+EDFSFY + +P  + ++G++N+    + +
Sbjct: 336 NDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMAS 395

Query: 444 GHSPYFMIDEDMLPIGAATHATIAERYLYE 474
            HSPYF ++E++LP GA+ HA++A RYL E
Sbjct: 396 PHSPYFEVNEELLPYGASLHASMATRYLLE 425

BLAST of Carg24434 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 438.3 bits (1126), Expect = 7.7e-123
Identity = 216/390 (55.38%), Postives = 283/390 (72.56%), Query Frame = 0

Query: 84  LSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPFAKTGVRAW 143
           LSLAKR D  DW+  +RRRIHENPEL +EE ETS+LV+ ELD+M  SY++P A TGV  +
Sbjct: 36  LSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGVSYKNPVAVTGVIGY 95

Query: 144 IGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAAKILKDREH 203
           +GTG  PFVALRADMDALPIQE VEWEHKS++PGKMHACGHDAH TMLLGAAK+LK+ + 
Sbjct: 96  VGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQE 155

Query: 204 LLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSRPGPLLAGC 263
            L+GTV+L+FQPAEE G GAK+++  G L NV AIF +HVS+ L    + SR G L+AG 
Sbjct: 156 ELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGLGQLSSREGLLMAGS 215

Query: 264 GFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSVTWFNGGSN 323
           G F+A I+GK   A  P  ++DPVLAAS  ++SLQ +VSREA+PLDSQVV+V  F G   
Sbjct: 216 GRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLDSQVVTVATFEGSDA 275

Query: 324 LDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFFEKEYTIYPPTV 383
            +VIPD+V IGGTFRA    SF QL QRI +VI  QA V  C+ TVDF E E   +PPTV
Sbjct: 276 FNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATVDFLEDETPPFPPTV 335

Query: 384 NDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFYSEFVPTAYYYIGVRNETLGSIHT 443
           N++ ++   K V+ D+ G  NY    P+M +EDF+FY + +P  + ++G++N++   +  
Sbjct: 336 NNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMAN 395

Query: 444 GHSPYFMIDEDMLPIGAATHATIAERYLYE 474
            HSP+F ++E++LP GA+  A++A RYL +
Sbjct: 396 PHSPFFEVNEELLPYGASLLASLATRYLLD 425

BLAST of Carg24434 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.3e-122
Identity = 217/398 (54.52%), Postives = 287/398 (72.11%), Query Frame = 0

Query: 76  TEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPF 135
           T      +L  AK P+V DW+ K+RR+IHENPEL +EE ETS+L+R EL+ +   Y +P 
Sbjct: 31  TSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSELELIGIKYRYPV 90

Query: 136 AKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAA 195
           A TGV  +IGTG PPFVALRADMDALPIQE VEWEHKS++ GKMHACGHD HVTMLLGAA
Sbjct: 91  AITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAA 150

Query: 196 KILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSR 255
           KIL +  H L+GTVVL+FQPAEE  +GAK+M  +GAL+NV+AIF +H+S  +P     SR
Sbjct: 151 KILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLSARIPFGKAASR 210

Query: 256 PGPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSV 315
            G  LAG G F AVITGK   A  P  ++DPV+AAS+ V+SLQ +VSRE +PLDS+VV+V
Sbjct: 211 AGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTV 270

Query: 316 TWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFFEKE 375
           +  NGG+  +VIPD++ IGGT RAF  + F QL QR++EVI +QA V+RC+ +V+     
Sbjct: 271 SKVNGGNAFNVIPDSITIGGTLRAF--TGFTQLQQRVKEVITKQAAVHRCNASVNLTPNG 330

Query: 376 YTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFYSEFVPTAYYYIGVRN 435
               PPTVN++ +Y+  KKV  DL G   +    P+MG+EDFS+++E +P  +  +G+++
Sbjct: 331 REPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQD 390

Query: 436 ETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYE 474
           ET G   + HSP + I+ED+LP GAA HA++A +YL E
Sbjct: 391 ETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQYLKE 425

BLAST of Carg24434 vs. TAIR 10
Match: AT3G02875.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 426.8 bits (1096), Expect = 2.3e-119
Identity = 209/399 (52.38%), Postives = 276/399 (69.17%), Query Frame = 0

Query: 77  EACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMDASYEHPFA 136
           E+ +  +L  AK P+  +W++ +RR+IHENPE  F+EF+TSQLVRDELD +   Y++P A
Sbjct: 33  ESLARGMLHSAKDPEFFEWMRGIRRKIHENPETGFQEFKTSQLVRDELDSLGVKYKYPVA 92

Query: 137 KTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMHACGHDAHVTMLLGAAK 196
           KTGV AWIG+   P   LRADMDALP+QE VEWE KS+V GKMHACGHD HV MLLGAAK
Sbjct: 93  KTGVVAWIGSCSKPVFGLRADMDALPLQELVEWESKSKVDGKMHACGHDTHVAMLLGAAK 152

Query: 197 ILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFAVHVSHELPTAIIGSRP 256
           +L+  +HL+KGTV L+FQP EE   GA  M+ D  L ++  I +VHV   +P+  IGSRP
Sbjct: 153 LLQTTKHLIKGTVKLVFQPGEEGYAGAYEMLKDEILDDLDGILSVHVFPSIPSGGIGSRP 212

Query: 257 GPLLAGCGFFRAVITGKKDQAGSPHRSVDPVLAASAAVISLQGIVSREANPLDSQVVSVT 316
           G +LAG G F   + G+   A +PH S DPVLAAS+AV++LQ IVSRE +PL++ VV+V 
Sbjct: 213 GTVLAGAGLFTVTVHGQGSHAATPHFSKDPVLAASSAVVALQQIVSRELDPLEAGVVTVG 272

Query: 317 WFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQARVYRCSTTVDFFEKEY 376
           +  GG   +VIP +   GGTFR+ SN     + +RI+E+   QA VYRC   V+F EK+ 
Sbjct: 273 YIEGGHAQNVIPQSAKFGGTFRSLSNDGLLFIQRRIKEISEAQASVYRCKAEVNFEEKKP 332

Query: 377 TIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFYSEFVPTAYYYIGVRNE 436
           +++P   NDE +YEH KKVA  + G +N+   P  MG EDFSF+++    A + +GV+NE
Sbjct: 333 SLHPVMNNDEGLYEHGKKVAEAMIGKNNFHDFPVTMGGEDFSFFTQKTKAAIFVLGVKNE 392

Query: 437 TLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHG 476
           TLG+    HSPYF +DE+ LP+GAA HA +A  YL EHG
Sbjct: 393 TLGAGKPLHSPYFFVDEEALPVGAALHAAMAVSYLDEHG 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032805.13.1e-275100.00IAA-amino acid hydrolase ILR1-like 6, partial [Cucurbita argyrosperma subsp. arg... [more]
XP_022955832.12.0e-27499.79IAA-amino acid hydrolase ILR1-like 6 [Cucurbita moschata][more]
KAG6602101.13.8e-27399.37IAA-amino acid hydrolase ILR1-like 6, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023533052.11.6e-27198.74IAA-amino acid hydrolase ILR1-like 6 [Cucurbita pepo subsp. pepo][more]
XP_022990038.12.5e-26998.32IAA-amino acid hydrolase ILR1-like 6 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8VYX01.7e-16770.02IAA-amino acid hydrolase ILR1-like 6 OS=Arabidopsis thaliana OX=3702 GN=ILL6 PE=... [more]
Q5Z6781.3e-15168.41IAA-amino acid hydrolase ILR1-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8S9S44.1e-13760.05IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q84XG91.2e-13659.80IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
Q5N8F21.6e-13359.64IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1GUP69.7e-27599.79IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita moschata OX=3662 GN=LOC1114577... [more]
A0A6J1JS371.2e-26998.32IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita maxima OX=3661 GN=LOC111487050... [more]
A0A6J1JWG31.3e-23986.55IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita maxima OX=3661 GN=LOC111489479... [more]
A0A6J1FFS93.8e-23986.76IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita moschata OX=3662 GN=LOC1114450... [more]
A0A0A0KDD23.9e-23185.08M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G384060 P... [more]
Match NameE-valueIdentityDescription
AT1G44350.11.2e-16870.02IAA-leucine resistant (ILR)-like gene 6 [more]
AT1G51760.14.8e-13357.69peptidase M20/M25/M40 family protein [more]
AT1G51780.17.7e-12355.38IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56660.11.3e-12254.52IAA-leucine resistant (ILR)-like 2 [more]
AT3G02875.12.3e-11952.38Peptidase M20/M25/M40 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 98..459
e-value: 6.1E-107
score: 355.9
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 52..474
NoneNo IPR availableGENE3D3.30.70.360coord: 261..379
e-value: 2.3E-131
score: 439.9
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 98..456
e-value: 2.3E-131
score: 439.9
NoneNo IPR availablePANTHERPTHR11014:SF62IAA-AMINO ACID HYDROLASE ILR1-LIKE 6coord: 52..474
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 91..473
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 153..468
e-value: 1.3E-35
score: 123.4
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 266..361
e-value: 1.2E-4
score: 21.9
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 96..471
e-value: 0.0
score: 675.959
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 262..371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24434-RACarg24434-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
biological_process GO:0009694 jasmonic acid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity