Carg24224 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24224
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	double-stranded RNA-binding protein 4-like isoform X2
Location	Carg_Chr14: 4479043 .. 4481506 (+)
RNA-Seq Expression	Carg24224
Synteny	Carg24224

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGACCGGAAATTTCCCCTGCTATTCATTCATTGCCATTGCCTAATTCTTCCCATTTCAATTTGTTCTCATCGAGTTTCGATAACATTCGATCATGGTGGAAGAATCCGGAACAGTTGAAGCAGTTCCGCAGCAACAATCTTCATTCCCGCCAGATTCGGCTCCGGCTGTTGTTCCAGCTGTTATTCCGGTGGAAAACTCTAATACTCCTCAGCTTGAATCGGATACTCGTCCTCCACCTGTTTTACCGGACGCAATTCTGATTTCGAGTTCGGCTTCAGCTCCGGTTTCGTCTCCGAAACCCTCTGATCTGACTCGTAAGTCTATGGTATTGTTGCCTATCATTGTTTTTTAGGCGTTTTGATTGTTACGGTTTAGTGTTTTTATTTGAGCTAATGCTGCTGTTAGATGATGGATTTCCTCTTCATTTGGTGCATGAAGTTTTCTAGCTGTTTTTTTTTTTTTTTTTTTCTGTTATAGTGAGAAGCAATTGCACTCCTTCTGTGAGTTCGCGTGGTGCCTGTCTGTGTGTTGAAAAGATAACTCTGTTTTTCTGTATGTATCTACGAAGAGGTCCTGAGAATTCGATCTTAAGTTGCTATGTTTACTTTCATTTTCTTTTCACAATCTTTGGACGCTGGATTATTTTGCGAGTTTGAATACTGGCAGCTGTGTGGTGATGATCTAGAGATTCCTTCTTTGTTCAGTTAAAAAAAATTGGTGGAAAAATTCTCTAGAAGAAATTGTTCTATGTGGTTCTTAGCGCTTCTTAATTGAATCGTAGCCTATCACCTTGCCCTCGATTTCTAGTGGTGTGGCATTCGCGAAAAGAATAGTGAGATTTAAATCACCTATTTATTCGTATTTCCTATACTGTTCCTACACTCTCTGGTGTGCTCGATGAGAGATGGATAAACTAAGGACTAATCACTTCAGGCACTAGCTGGTCCTCATTGGAGCAACAAACCCGAATTCGGTGGGGGTTTACTGGCATAGCTCTCTGTTTTGATCTGACAATGAGGACTCTTTCTTTGAAGACCAGTTTCTTGACAATCCTGTGGTGGGTTTAACTCGTAAGTAACCAGATAAGCCTCGAAGAAGTGAAAGATAAATGGGAGGATGTTCTGAATTTTGATCATTTTATTATTGTTCCTTTTCTTTTGCATCTTCCTTGATTTTTCTATTTTCTGTGATTAAAGATGTAGGAGCCTTTTCTTATAATTTCTTTTGCTAGTTCTGCATCTTGGTTTGGCACTCAGGGCTTTGTTATTGTATGGCCTTTTTCTACTTCCATTGCCTTCAACGAAAGTATTAAAAAAGAAAGGAAAAAAAGACGAAGTCATTTACTGAAGTGGAGAGTTTTGCAATATTGGACGTGTCTGGGTACTTATCTGATCTGACGTTGCTGATGGGGGACTTTTGTAATTTTCCACAGCATGGACTCTTCTAATGTCCCGCCTTTCTCTTTCGGGCTGTAATTTGATTATTATATTTTAATCTAGTTGCCTAATAAAAGTAAAGGTTCCCCCACAGTTCTAGTGTGCAGAAATTTGTTATTTGGTGGATCTTTGTTTTCTTGATAAGAGCAGAAAAGAATTAATGTTATGACTTTCTTGTACTTGGTTTTGCAGAACTCTTCCTGTACAAAAATCATTTGCATGAGTACACTCAAAAGTCAAGGATACCATTACCAGTATATCAGACTTTTGATGAAGGATCTCAAGGCGTACCTAAATATAGGTCAACTGTTGTGGTTGATGAGGTACATTATGTGTCTCCTAATACGTTCCGTAATCGAAGGGCAGCTGAACAAGATGCTGCAAGAATTGCCCTCGAGTACATATCAAAAAAGGCTAAGGATGATGGATTTGTTCTCCTTCATGAGGTATGACTTATATGATGAGACTGAAACTTGTTTGGTGGCCTTTGATTTTTAGCATGCTTGTTTCTTTATGTATTTTTTTTTATTTTAGGCAACATTGTGGGGGAAATTTAAACCTTCTGAACTTAATGGAAGAAATAGATGCTTTGACCACTATATTATGCTCATGTTGGCTATATTTTTGCTTGTTTAGTTGATTATAGACGCAATTCAATGATGAGATTTCCTACCTAGACTTTCAGTTTGTTACAAATAAGTTTTTATGTATGGAGATTACCTTTTGTAATTTTATTTCGCAATGTGTACAATAACTTATTTGAATCCAACAGAAACAGTAATTTCTACCCGATATATTTGTTATTGTTCTTGGATGAAAAAATCTTAGTAATAATTGCATGTAATCACTTAAGTTGTATAAGATTTCTCATAGTCATAGTCATCGACATTCTCGAAGGTTGGAATTTATCTAATTGATTTTAGTGATAAGGCATTTAGTCTGTCTGAATAAAAAGTTCAGAAATGATGCCTTCGGGAATGAGGTTGTGAGACACTGTTTGCTTTTGTTTGTAGTTCACAGGGATAG

mRNA sequence

CAGACCGGAAATTTCCCCTGCTATTCATTCATTGCCATTGCCTAATTCTTCCCATTTCAATTTGTTCTCATCGAGTTTCGATAACATTCGATCATGGTGGAAGAATCCGGAACAGTTGAAGCAGTTCCGCAGCAACAATCTTCATTCCCGCCAGATTCGGCTCCGGCTGTTGTTCCAGCTGTTATTCCGGTGGAAAACTCTAATACTCCTCAGCTTGAATCGGATACTCGTCCTCCACCTGTTTTACCGGACGCAATTCTGATTTCGAGTTCGGCTTCAGCTCCGGTTTCGTCTCCGAAACCCTCTGATCTGACTCAACTCTTCCTGTACAAAAATCATTTGCATGAGTACACTCAAAAGTCAAGGATACCATTACCAGTATATCAGACTTTTGATGAAGGATCTCAAGGCGTACCTAAATATAGGTCAACTGTTGTGGTTGATGAGGTACATTATGTGTCTCCTAATACGTTCCGTAATCGAAGGGCAGCTGAACAAGATGCTGCAAGAATTGCCCTCGAGTACATATCAAAAAAGGCTAAGGATGATGGATTTGTTCTCCTTCATGAGGGATAG

Coding sequence (CDS)

ATGGTGGAAGAATCCGGAACAGTTGAAGCAGTTCCGCAGCAACAATCTTCATTCCCGCCAGATTCGGCTCCGGCTGTTGTTCCAGCTGTTATTCCGGTGGAAAACTCTAATACTCCTCAGCTTGAATCGGATACTCGTCCTCCACCTGTTTTACCGGACGCAATTCTGATTTCGAGTTCGGCTTCAGCTCCGGTTTCGTCTCCGAAACCCTCTGATCTGACTCAACTCTTCCTGTACAAAAATCATTTGCATGAGTACACTCAAAAGTCAAGGATACCATTACCAGTATATCAGACTTTTGATGAAGGATCTCAAGGCGTACCTAAATATAGGTCAACTGTTGTGGTTGATGAGGTACATTATGTGTCTCCTAATACGTTCCGTAATCGAAGGGCAGCTGAACAAGATGCTGCAAGAATTGCCCTCGAGTACATATCAAAAAAGGCTAAGGATGATGGATTTGTTCTCCTTCATGAGGGATAG

Protein sequence

MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSSASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVHYVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHEG

Homology

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BLAST of Carg24224 vs. NCBI nr
Match: KAG7017947.1 (Double-stranded RNA-binding protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 312.8 bits (800), Expect = 1.8e-81
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHEG 161
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHEG
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHEG 160

[Close]

BLAST of Carg24224 vs. NCBI nr
Match: XP_022935533.1 (double-stranded RNA-binding protein 4-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 304.7 bits (779), Expect = 4.8e-79
Identity = 156/159 (98.11%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSA AVVPAVIPVENSNTPQLESDTRPPPVLPDAILI SS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSASAVVPAVIPVENSNTPQLESDTRPPPVLPDAILIPSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQG+PKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGIPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

[Close]

BLAST of Carg24224 vs. NCBI nr
Match: XP_022935532.1 (double-stranded RNA-binding protein 4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 304.7 bits (779), Expect = 4.8e-79
Identity = 156/159 (98.11%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSA AVVPAVIPVENSNTPQLESDTRPPPVLPDAILI SS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSASAVVPAVIPVENSNTPQLESDTRPPPVLPDAILIPSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQG+PKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGIPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

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BLAST of Carg24224 vs. NCBI nr
Match: XP_023526614.1 (double-stranded RNA-binding protein 4-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526615.1 double-stranded RNA-binding protein 4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 303.9 bits (777), Expect = 8.1e-79
Identity = 156/159 (98.11%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPP VLPDAILI S+
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPSVLPDAILIPST 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

[Close]

BLAST of Carg24224 vs. NCBI nr
Match: KAG6581214.1 (Double-stranded RNA-binding protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 302.4 bits (773), Expect = 2.4e-78
Identity = 155/159 (97.48%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVL DAILI SS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLLDAILIPSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHE+TQKSRIPLPVYQTFDEGSQG+PKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEFTQKSRIPLPVYQTFDEGSQGIPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

[Close]

BLAST of Carg24224 vs. ExPASy Swiss-Prot
Match: O04492 (Double-stranded RNA-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=DRB1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.9e-10
Identity = 33/85 (38.82%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 66  SSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVHYVSPN 125
           S+   S ++  +++K+ L EY QK ++P PVY+   EG      ++STV++D V Y S  
Sbjct: 3   STDVSSGVSNCYVFKSRLQEYAQKYKLPTPVYEIVKEGPSHKSLFQSTVILDGVRYNSLP 62

Query: 126 TFRNRRAAEQDAARIALEYISKKAK 151
            F NR+AAEQ AA +AL  ++K ++
Sbjct: 63  GFFNRKAAEQSAAEVALRELAKSSE 87

[Close]

BLAST of Carg24224 vs. ExPASy Swiss-Prot
Match: Q0DJA3 (Double-stranded RNA-binding protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=DRB3 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 36/107 (33.64%), Postives = 60/107 (56.07%), Query Frame = 0


Query: 41  LESDTRPPPVLPDAILISSSASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTF 100
           ++  + P P+ P+    ++++ AP+ +     +   +++K+ L EY QK+ +  P Y T 
Sbjct: 1   MKKKSAPTPLPPET---ANTSPAPIGATAGIRVENCYVFKSRLQEYAQKAGLQTPEYHTS 60

Query: 101 DEGSQGVPKYRSTVVVDEVHYVSPNTFRNRRAAEQDAARIALEYISK 148
            EG    P ++STVV++   Y S   F NR+AAEQ AA +AL  I K
Sbjct: 61  KEGPSHEPVFKSTVVINNTSYGSLPGFSNRKAAEQSAAEVALMEIVK 104

[Close]

BLAST of Carg24224 vs. ExPASy Swiss-Prot
Match: Q0IV63 (Double-stranded RNA-binding protein 7 OS=Oryza sativa subsp. japonica OX=39947 GN=DRB7 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 38/104 (36.54%), Postives = 58/104 (55.77%), Query Frame = 0


Query: 44  DTRPPPVLPDAILISSSASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEG 103
           D  P P+ P+    ++++ AP  +     +   +++K+ L EY QK+ +  P Y TF EG
Sbjct: 2   DMPPTPLPPET---ANTSPAPNGATAGIRVENCYVFKSRLQEYAQKAGLQTPEYHTFKEG 61

Query: 104 SQGVPKYRSTVVVDEVHYVSPNTFRNRRAAEQDAARIALEYISK 148
               P ++STVV++   Y S   F NR+AAEQ AA +AL  I K
Sbjct: 62  PSHEPVFKSTVVINNTSYDSLPGFFNRKAAEQSAAEVALMEIVK 102

[Close]

BLAST of Carg24224 vs. ExPASy Swiss-Prot
Match: Q0IQN6 (Double-stranded RNA-binding protein 8 OS=Oryza sativa subsp. japonica OX=39947 GN=DRB8 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 38/104 (36.54%), Postives = 58/104 (55.77%), Query Frame = 0


Query: 44  DTRPPPVLPDAILISSSASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEG 103
           D  P P+ P+    ++++ AP  +     +   +++K+ L EY QK+ +  P Y TF EG
Sbjct: 2   DMPPTPLPPET---ANTSPAPNGATAGIRVENCYVFKSRLQEYAQKTGLQTPEYHTFKEG 61

Query: 104 SQGVPKYRSTVVVDEVHYVSPNTFRNRRAAEQDAARIALEYISK 148
               P ++STVV++   Y S   F NR+AAEQ AA +AL  I K
Sbjct: 62  PSHEPVFKSTVVINNTSYDSLPGFFNRKAAEQSAAEVALMEIVK 102

[Close]

BLAST of Carg24224 vs. ExPASy Swiss-Prot
Match: Q5N8Z0 (Double-stranded RNA-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DRB1 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.2e-05
Identity = 32/89 (35.96%), Postives = 45/89 (50.56%), Query Frame = 0


Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           +S P+  P P    Q   YK+ L  Y QK    LP YQT  EG     +++S V VD   
Sbjct: 70  SSLPLPPPPPPSENQ-SSYKSQLQIYAQKKGKLLPSYQTIREGPGHASRFKSVVTVDGKA 129

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKA 150
           + SP  F   + AE  AA++AL  + ++A
Sbjct: 130 FESPEYFHTVKEAESAAAKLALMSLPQEA 157

[Close]

BLAST of Carg24224 vs. ExPASy TrEMBL
Match: A0A6J1F5U3 (double-stranded RNA-binding protein 4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442377 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.3e-79
Identity = 156/159 (98.11%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSA AVVPAVIPVENSNTPQLESDTRPPPVLPDAILI SS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSASAVVPAVIPVENSNTPQLESDTRPPPVLPDAILIPSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQG+PKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGIPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

[Close]

BLAST of Carg24224 vs. ExPASy TrEMBL
Match: A0A6J1FAT0 (double-stranded RNA-binding protein 4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442377 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.3e-79
Identity = 156/159 (98.11%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSA AVVPAVIPVENSNTPQLESDTRPPPVLPDAILI SS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSASAVVPAVIPVENSNTPQLESDTRPPPVLPDAILIPSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQG+PKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGIPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

[Close]

BLAST of Carg24224 vs. ExPASy TrEMBL
Match: A0A6J1J9P9 (double-stranded RNA-binding protein 4-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482525 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.5e-78
Identity = 154/159 (96.86%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSAPAVVPA+IPVE+SNTPQLESDTRPPPVLP AILI SS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPALIPVEDSNTPQLESDTRPPPVLPGAILIPSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQG+PKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGIPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

[Close]

BLAST of Carg24224 vs. ExPASy TrEMBL
Match: A0A6J1J1D4 (double-stranded RNA-binding protein 4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482525 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.5e-78
Identity = 154/159 (96.86%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSAPAVVPA+IPVE+SNTPQLESDTRPPPVLP AILI SS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPALIPVEDSNTPQLESDTRPPPVLPGAILIPSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQG+PKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGIPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

[Close]

BLAST of Carg24224 vs. ExPASy TrEMBL
Match: A0A6J1J4E3 (double-stranded RNA-binding protein 4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482525 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.5e-78
Identity = 154/159 (96.86%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPAVIPVENSNTPQLESDTRPPPVLPDAILISSS 60
           MVEESGTVEAVPQQQSSFPPDSAPAVVPA+IPVE+SNTPQLESDTRPPPVLP AILI SS
Sbjct: 1   MVEESGTVEAVPQQQSSFPPDSAPAVVPALIPVEDSNTPQLESDTRPPPVLPGAILIPSS 60

Query: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVH 120
           ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQG+PKYRSTVVVDEVH
Sbjct: 61  ASAPVSSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGIPKYRSTVVVDEVH 120

Query: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 160
           YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE
Sbjct: 121 YVSPNTFRNRRAAEQDAARIALEYISKKAKDDGFVLLHE 159

[Close]

BLAST of Carg24224 vs. TAIR 10
Match: AT1G09700.1 (dsRNA-binding domain-like superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.0e-11
Identity = 33/85 (38.82%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 66  SSPKPSDLTQLFLYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVHYVSPN 125
           S+   S ++  +++K+ L EY QK ++P PVY+   EG      ++STV++D V Y S  
Sbjct: 3   STDVSSGVSNCYVFKSRLQEYAQKYKLPTPVYEIVKEGPSHKSLFQSTVILDGVRYNSLP 62

Query: 126 TFRNRRAAEQDAARIALEYISKKAK 151
            F NR+AAEQ AA +AL  ++K ++
Sbjct: 63  GFFNRKAAEQSAAEVALRELAKSSE 87

[Close]

BLAST of Carg24224 vs. TAIR 10
Match: AT2G28380.1 (dsRNA-binding protein 2 )

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-05
Identity = 25/71 (35.21%), Postives = 36/71 (50.70%), Query Frame = 0


Query: 78  LYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVHYVSPNTFRNRRAAEQDA 137
           +YKN L E  Q+S   LP Y    EG    P++++TV  +   + SP      R AE  A
Sbjct: 1   MYKNQLQELAQRSCFNLPSYTCIREGPDHAPRFKATVNFNGEIFESPQYCSTLRQAEHSA 60

Query: 138 ARIALEYISKK 149
           A +AL  +S +
Sbjct: 61  AEVALNALSNR 71

[Close]

BLAST of Carg24224 vs. TAIR 10
Match: AT3G26932.1 (dsRNA-binding protein 3 )

HSP 1 Score: 46.6 bits (109), Expect = 2.2e-05
Identity = 25/71 (35.21%), Postives = 36/71 (50.70%), Query Frame = 0


Query: 78  LYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVHYVSPNTFRNRRAAEQDA 137
           +YKN L E  Q+S   LP Y    EG    P+++++V  +   + SP      R AE  A
Sbjct: 1   MYKNQLQELAQRSCFSLPSYTCTREGPDHAPRFKASVNFNGEIFESPTYCSTLRQAEHSA 60

Query: 138 ARIALEYISKK 149
           A +AL  +S K
Sbjct: 61  AEVALSALSSK 71

[Close]

BLAST of Carg24224 vs. TAIR 10
Match: AT3G26932.2 (dsRNA-binding protein 3 )

HSP 1 Score: 46.6 bits (109), Expect = 2.2e-05
Identity = 25/71 (35.21%), Postives = 36/71 (50.70%), Query Frame = 0


Query: 78  LYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVHYVSPNTFRNRRAAEQDA 137
           +YKN L E  Q+S   LP Y    EG    P+++++V  +   + SP      R AE  A
Sbjct: 1   MYKNQLQELAQRSCFSLPSYTCTREGPDHAPRFKASVNFNGEIFESPTYCSTLRQAEHSA 60

Query: 138 ARIALEYISKK 149
           A +AL  +S K
Sbjct: 61  AEVALSALSSK 71

[Close]

BLAST of Carg24224 vs. TAIR 10
Match: AT5G41070.1 (dsRNA-binding protein 5 )

HSP 1 Score: 44.3 bits (103), Expect = 1.1e-04
Identity = 23/71 (32.39%), Postives = 36/71 (50.70%), Query Frame = 0


Query: 78  LYKNHLHEYTQKSRIPLPVYQTFDEGSQGVPKYRSTVVVDEVHYVSPNTFRNRRAAEQDA 137
           +YKN L E  Q+S   LP Y    EG    P+++++V  +   + SP      R AE  A
Sbjct: 1   MYKNQLQELAQRSCFNLPSYTCIREGPDHAPRFKASVNFNGEIFESPTYCSTLRQAEHAA 60

Query: 138 ARIALEYISKK 149
           A ++L  +S +
Sbjct: 61  AEVSLNVLSSR 71

The following BLAST results are available for this feature:

BLAST of Carg24224 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017947.1	1.8e-81	100.00	Double-stranded RNA-binding protein 1 [Cucurbita argyrosperma subsp. argyrosperm...	[more]
XP_022935533.1	4.8e-79	98.11	double-stranded RNA-binding protein 4-like isoform X2 [Cucurbita moschata]	[more]
XP_022935532.1	4.8e-79	98.11	double-stranded RNA-binding protein 4-like isoform X1 [Cucurbita moschata]	[more]
XP_023526614.1	8.1e-79	98.11	double-stranded RNA-binding protein 4-like isoform X1 [Cucurbita pepo subsp. pep...	[more]
KAG6581214.1	2.4e-78	97.48	Double-stranded RNA-binding protein 4, partial [Cucurbita argyrosperma subsp. so...	[more]

BLAST of Carg24224 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04492	2.9e-10	38.82	Double-stranded RNA-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=DRB1 PE...	[more]
Q0DJA3	1.1e-09	33.64	Double-stranded RNA-binding protein 3 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q0IV63	1.1e-09	36.54	Double-stranded RNA-binding protein 7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q0IQN6	1.1e-09	36.54	Double-stranded RNA-binding protein 8 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q5N8Z0	1.2e-05	35.96	Double-stranded RNA-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]

BLAST of Carg24224 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F5U3	2.3e-79	98.11	double-stranded RNA-binding protein 4-like isoform X1 OS=Cucurbita moschata OX=3...	[more]
A0A6J1FAT0	2.3e-79	98.11	double-stranded RNA-binding protein 4-like isoform X2 OS=Cucurbita moschata OX=3...	[more]
A0A6J1J9P9	1.5e-78	96.86	double-stranded RNA-binding protein 4-like isoform X3 OS=Cucurbita maxima OX=366...	[more]
A0A6J1J1D4	1.5e-78	96.86	double-stranded RNA-binding protein 4-like isoform X2 OS=Cucurbita maxima OX=366...	[more]
A0A6J1J4E3	1.5e-78	96.86	double-stranded RNA-binding protein 4-like isoform X1 OS=Cucurbita maxima OX=366...	[more]

BLAST of Carg24224 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09700.1	2.0e-11	38.82	dsRNA-binding domain-like superfamily protein	[more]
AT2G28380.1	1.3e-05	35.21	dsRNA-binding protein 2	[more]
AT3G26932.1	2.2e-05	35.21	dsRNA-binding protein 3	[more]
AT3G26932.2	2.2e-05	35.21	dsRNA-binding protein 3	[more]
AT5G41070.1	1.1e-04	32.39	dsRNA-binding protein 5	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 79..146 e-value: 4.9E-13 score: 59.3
IPR014720	Double-stranded RNA-binding domain	PFAM	PF00035	dsrm	coord: 79..144 e-value: 2.6E-10 score: 40.8
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 78..147 score: 11.552683
None	No IPR available	GENE3D	3.30.160.20		coord: 62..150 e-value: 1.6E-19 score: 71.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..53
None	No IPR available	PANTHER	PTHR11207:SF26	DOUBLE-STRANDED RNA-BINDING PROTEIN 4	coord: 62..159
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 74..145
IPR011907	Ribonuclease III	PANTHER	PTHR11207	RIBONUCLEASE III	coord: 62..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24224-RA	Carg24224-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0016075	rRNA catabolic process
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding