Carg24175 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24175
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like
Location	Carg_Chr06: 6238499 .. 6238723 (+)
RNA-Seq Expression	Carg24175
Synteny	Carg24175

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAATAAGCCTTTGGGATCGACTGGAGAGTTCTTCAGGAGGACAGACGAGTGGAGGAAGCATCCGATGCTCTCCAATCAGTTCCGACATGCCACTCCTGGCCTCGGCATCGCTCTCGTTGCTTTCGGCATCTACCTTGTCGGCGAGCAAGTATATAACAGGATCCATTCTCCTTCTTCCTCTCGCCATCACCATGCTGATGCTTCCTCCGCCACCCATTAA

mRNA sequence

ATGGCGAATAAGCCTTTGGGATCGACTGGAGAGTTCTTCAGGAGGACAGACGAGTGGAGGAAGCATCCGATGCTCTCCAATCAGTTCCGACATGCCACTCCTGGCCTCGGCATCGCTCTCGTTGCTTTCGGCATCTACCTTGTCGGCGAGCAAGTATATAACAGGATCCATTCTCCTTCTTCCTCTCGCCATCACCATGCTGATGCTTCCTCCGCCACCCATTAA

Coding sequence (CDS)

ATGGCGAATAAGCCTTTGGGATCGACTGGAGAGTTCTTCAGGAGGACAGACGAGTGGAGGAAGCATCCGATGCTCTCCAATCAGTTCCGACATGCCACTCCTGGCCTCGGCATCGCTCTCGTTGCTTTCGGCATCTACCTTGTCGGCGAGCAAGTATATAACAGGATCCATTCTCCTTCTTCCTCTCGCCATCACCATGCTGATGCTTCCTCCGCCACCCATTAA

Protein sequence

MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPSSSRHHHADASSATH

Homology

[Close]

BLAST of Carg24175 vs. NCBI nr
Match: KAG7028500.1 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 157.1 bits (396), Expect = 5.7e-35
Identity = 74/74 (100.00%), Postives = 74/74 (100.00%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS
Sbjct: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60

Query: 61 SSRHHHADASSATH 75
          SSRHHHADASSATH
Sbjct: 61 SSRHHHADASSATH 74

[Close]

BLAST of Carg24175 vs. NCBI nr
Match: XP_022950932.1 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like [Cucurbita moschata])

HSP 1 Score: 155.2 bits (391), Expect = 2.2e-34
Identity = 73/74 (98.65%), Postives = 73/74 (98.65%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS
Sbjct: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHHADASSATH
Sbjct: 61 SSHHHHADASSATH 74

[Close]

BLAST of Carg24175 vs. NCBI nr
Match: XP_022974255.1 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like [Cucurbita maxima])

HSP 1 Score: 154.1 bits (388), Expect = 4.8e-34
Identity = 72/74 (97.30%), Postives = 73/74 (98.65%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRRTDEWRKHPML+NQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS
Sbjct: 1  MANKPLGSTGEFFRRTDEWRKHPMLTNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHHADASSATH
Sbjct: 61 SSHHHHADASSATH 74

[Close]

BLAST of Carg24175 vs. NCBI nr
Match: XP_023540348.1 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 150.2 bits (378), Expect = 7.0e-33
Identity = 70/74 (94.59%), Postives = 71/74 (95.95%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRRTDEWRKHP L+NQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS
Sbjct: 1  MANKPLGSTGEFFRRTDEWRKHPTLTNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHHAD SSATH
Sbjct: 61 SSHHHHADVSSATH 74

[Close]

BLAST of Carg24175 vs. NCBI nr
Match: XP_008460580.1 (PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B [Cucumis melo] >XP_008460581.1 PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B [Cucumis melo])

HSP 1 Score: 147.9 bits (372), Expect = 3.4e-32
Identity = 69/74 (93.24%), Postives = 72/74 (97.30%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRR DEWRKHPML+NQFRHATPGLGIALVAFGIYLVGEQVYNRI+SPS
Sbjct: 1  MANKPLGSTGEFFRRRDEWRKHPMLTNQFRHATPGLGIALVAFGIYLVGEQVYNRINSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHH+DASSATH
Sbjct: 61 SSHHHHSDASSATH 74

[Close]

BLAST of Carg24175 vs. ExPASy Swiss-Prot
Match: Q9M9R9 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B OS=Arabidopsis thaliana OX=3702 GN=At1g14450 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 6.1e-24
Identity = 48/71 (67.61%), Postives = 58/71 (81.69%), Query Frame = 0


Query: 4  KPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPSSSR 63
          KPLG+TGEFFRR DEWRKHPMLSNQ RHA PGLGI + AF +YLVGEQ+YN+  +PS S 
Sbjct: 3  KPLGTTGEFFRRRDEWRKHPMLSNQMRHALPGLGIGVAAFCVYLVGEQIYNKALAPSKSS 62

Query: 64 HHHADASSATH 75
          HHH + ++ +H
Sbjct: 63 HHHQEQTAPSH 73

[Close]

BLAST of Carg24175 vs. ExPASy Swiss-Prot
Match: O64725 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A OS=Arabidopsis thaliana OX=3702 GN=At2g02510 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.4e-20
Identity = 43/62 (69.35%), Postives = 52/62 (83.87%), Query Frame = 0


Query: 4  KPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPSSSR 63
          KPLG+TGEFFRR DEWRKHPMLSNQ RHA PG+GI + AF +YLVGEQ+Y+++ +PSS  
Sbjct: 3  KPLGTTGEFFRRRDEWRKHPMLSNQMRHALPGIGIGVGAFCVYLVGEQIYSKLMAPSSQS 62

Query: 64 HH 66
           H
Sbjct: 63 SH 64

[Close]

BLAST of Carg24175 vs. ExPASy TrEMBL
Match: A0A6J1GG90 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like OS=Cucurbita moschata OX=3662 GN=LOC111453875 PE=3 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.0e-34
Identity = 73/74 (98.65%), Postives = 73/74 (98.65%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS
Sbjct: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHHADASSATH
Sbjct: 61 SSHHHHADASSATH 74

[Close]

BLAST of Carg24175 vs. ExPASy TrEMBL
Match: A0A6J1IDH2 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like OS=Cucurbita maxima OX=3661 GN=LOC111472888 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.3e-34
Identity = 72/74 (97.30%), Postives = 73/74 (98.65%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRRTDEWRKHPML+NQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS
Sbjct: 1  MANKPLGSTGEFFRRTDEWRKHPMLTNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHHADASSATH
Sbjct: 61 SSHHHHADASSATH 74

[Close]

BLAST of Carg24175 vs. ExPASy TrEMBL
Match: A0A1S3CD83 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B OS=Cucumis melo OX=3656 GN=LOC103499369 PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.7e-32
Identity = 69/74 (93.24%), Postives = 72/74 (97.30%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRR DEWRKHPML+NQFRHATPGLGIALVAFGIYLVGEQVYNRI+SPS
Sbjct: 1  MANKPLGSTGEFFRRRDEWRKHPMLTNQFRHATPGLGIALVAFGIYLVGEQVYNRINSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHH+DASSATH
Sbjct: 61 SSHHHHSDASSATH 74

[Close]

BLAST of Carg24175 vs. ExPASy TrEMBL
Match: A0A6J1KDK9 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like OS=Cucurbita maxima OX=3661 GN=LOC111493369 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.8e-32
Identity = 69/74 (93.24%), Postives = 71/74 (95.95%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRR DEWRKHP L+NQFRHATPGLGIALVAFGIYLVGEQVYNRI+SPS
Sbjct: 1  MANKPLGSTGEFFRRRDEWRKHPSLTNQFRHATPGLGIALVAFGIYLVGEQVYNRIYSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHHADASSATH
Sbjct: 61 SSHHHHADASSATH 74

[Close]

BLAST of Carg24175 vs. ExPASy TrEMBL
Match: A0A0A0KR92 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G528700 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.8e-32
Identity = 69/74 (93.24%), Postives = 72/74 (97.30%), Query Frame = 0


Query: 1  MANKPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPS 60
          MANKPLGSTGEFFRR DEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRI+SPS
Sbjct: 1  MANKPLGSTGEFFRRRDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRINSPS 60

Query: 61 SSRHHHADASSATH 75
          SS HHH++ASSATH
Sbjct: 61 SSHHHHSNASSATH 74

[Close]

BLAST of Carg24175 vs. TAIR 10
Match: AT1G14450.1 (NADH dehydrogenase (ubiquinone)s )

HSP 1 Score: 110.9 bits (276), Expect = 4.4e-25
Identity = 48/71 (67.61%), Postives = 58/71 (81.69%), Query Frame = 0


Query: 4  KPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPSSSR 63
          KPLG+TGEFFRR DEWRKHPMLSNQ RHA PGLGI + AF +YLVGEQ+YN+  +PS S 
Sbjct: 3  KPLGTTGEFFRRRDEWRKHPMLSNQMRHALPGLGIGVAAFCVYLVGEQIYNKALAPSKSS 62

Query: 64 HHHADASSATH 75
          HHH + ++ +H
Sbjct: 63 HHHQEQTAPSH 73

[Close]

BLAST of Carg24175 vs. TAIR 10
Match: AT2G02510.1 (NADH dehydrogenase (ubiquinone)s )

HSP 1 Score: 99.8 bits (247), Expect = 1.0e-21
Identity = 43/62 (69.35%), Postives = 52/62 (83.87%), Query Frame = 0


Query: 4  KPLGSTGEFFRRTDEWRKHPMLSNQFRHATPGLGIALVAFGIYLVGEQVYNRIHSPSSSR 63
          KPLG+TGEFFRR DEWRKHPMLSNQ RHA PG+GI + AF +YLVGEQ+Y+++ +PSS  
Sbjct: 3  KPLGTTGEFFRRRDEWRKHPMLSNQMRHALPGIGIGVGAFCVYLVGEQIYSKLMAPSSQS 62

Query: 64 HH 66
           H
Sbjct: 63 SH 64

The following BLAST results are available for this feature:

BLAST of Carg24175 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028500.1	5.7e-35	100.00	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B, partial [Cucurbit...	[more]
XP_022950932.1	2.2e-34	98.65	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like [Cucurbita mo...	[more]
XP_022974255.1	4.8e-34	97.30	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like [Cucurbita ma...	[more]
XP_023540348.1	7.0e-33	94.59	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like [Cucurbita pe...	[more]
XP_008460580.1	3.4e-32	93.24	PREDICTED: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B [Cucumi...	[more]

BLAST of Carg24175 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M9R9	6.1e-24	67.61	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B OS=Arabidopsis tha...	[more]
O64725	1.4e-20	69.35	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A OS=Arabidopsis tha...	[more]

BLAST of Carg24175 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GG90	1.0e-34	98.65	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like OS=Cucurbita ...	[more]
A0A6J1IDH2	2.3e-34	97.30	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like OS=Cucurbita ...	[more]
A0A1S3CD83	1.7e-32	93.24	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B OS=Cucumis melo OX...	[more]
A0A6J1KDK9	2.8e-32	93.24	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B-like OS=Cucurbita ...	[more]
A0A0A0KR92	2.8e-32	93.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G528700 PE=3 SV=1	[more]

BLAST of Carg24175 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14450.1	4.4e-25	67.61	NADH dehydrogenase (ubiquinone)s	[more]
AT2G02510.1	1.0e-21	69.35	NADH dehydrogenase (ubiquinone)s	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012576	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3	PFAM	PF08122	NDUF_B12	coord: 15..66 e-value: 5.0E-7 score: 29.6
IPR012576	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3	PANTHER	PTHR15082	NADH-UBIQUINONE OXIDOREDUCTASE B12 SUBUNIT	coord: 3..73
None	No IPR available	PANTHER	PTHR15082:SF4	NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 3-B	coord: 3..73

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24175-RA	Carg24175-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
biological_process	GO:0032981	mitochondrial respiratory chain complex I assembly
cellular_component	GO:0005747	mitochondrial respiratory chain complex I
cellular_component	GO:0005739	mitochondrion