Carg24083 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg24083 vs. NCBI nr
Match: KAG7015479.1 (Vacuolar protein sorting-associated protein 51-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 770/770 (100.00%), Postives = 770/770 (100.00%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSEIAASFSGGGTRGYEYGPAFVPAE 540
           LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSEIAASFSGGGTRGYEYGPAFVPAE
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSEIAASFSGGGTRGYEYGPAFVPAE 540

Query: 541 ICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAI 600
           ICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAI
Sbjct: 541 ICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAI 600

Query: 601 GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQ 660
           GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQ
Sbjct: 601 GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQ 660

Query: 661 KIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIAD 720
           KIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIAD
Sbjct: 661 KIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIAD 720

Query: 721 DEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNPTSP 771
           DEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNPTSP
Sbjct: 721 DEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNPTSP 770

BLAST of Carg24083 vs. NCBI nr
Match: KAG6577400.1 (Vacuolar protein sorting-associated protein 51-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1436.4 bits (3717), Expect = 0.0e+00
Identity = 767/783 (97.96%), Postives = 768/783 (98.08%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNG MDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGN SESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS             EIAASFSGGGT
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 780

BLAST of Carg24083 vs. NCBI nr
Match: XP_023552385.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552386.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552387.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552388.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1434.5 bits (3712), Expect = 0.0e+00
Identity = 767/783 (97.96%), Postives = 768/783 (98.08%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGN SESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNASESVYGASHEASV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS             EIAASFSGGGT
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAK QNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKYQNP 780

BLAST of Carg24083 vs. NCBI nr
Match: XP_022929381.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929382.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929383.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929384.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 763/783 (97.45%), Postives = 765/783 (97.70%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNG MDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGN SESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDA EQFIRKQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS             EIAASFSGGGT
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQNP 780

BLAST of Carg24083 vs. NCBI nr
Match: XP_022985178.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita maxima] >XP_022985179.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita maxima] >XP_022985180.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita maxima] >XP_022985181.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 1425.6 bits (3689), Expect = 0.0e+00
Identity = 759/783 (96.93%), Postives = 766/783 (97.83%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGN SESVYGASHE SV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEGSV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHF+AIEQFI+KQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFNAIEQFIKKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTC FSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCMFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKNSS+SQSQVLPEATQAEKVVAGLVLVLAQ+S             EIAASFSGGGT
Sbjct: 481 LLSGKNSSYSQSQVLPEATQAEKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEF+RLQTFNRSGFQQVQLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFIRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLI+AKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIKAKLAKAKDQNP 780

BLAST of Carg24083 vs. ExPASy Swiss-Prot
Match: Q0WQ75 (Vacuolar protein sorting-associated protein 51 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS51 PE=1 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 3.6e-276
Identity = 520/785 (66.24%), Postives = 624/785 (79.49%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPD--ASTSGSSMGSSNRYASPLEAINTTSFNPDQYM 60
           M  +   MDEK KRMRDLLSSFY+PD   STSGSS+ +S       + IN+TSF+ DQYM
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSINAS------FDNINSTSFDADQYM 60

Query: 61  SILVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMET 120
            ++++KSNLE LLQ+HV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME 
Sbjct: 61  DLMIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEG 120

Query: 121 NMEQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTE 180
           NM+QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+E
Sbjct: 121 NMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSE 180

Query: 181 AYADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAA 240
           AY DAVRFYTGAMPI KVYGD+SFQDC+RASEEAI I++KNLQ KLFSDSESIQ RAEAA
Sbjct: 181 AYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAA 240

Query: 241 VLLKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGAS-HE 300
           VLLKQLD PVDSLKAKLLEKLEQS   LQ+  E+ S+ +    S +   S   + A  HE
Sbjct: 241 VLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHE 300

Query: 301 ASVREFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIW 360
            +VR F+EAIRAYR IF DS+ +L KLA+ L   HF+ +E +I+K++ AAD L +F I+W
Sbjct: 301 DAVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVW 360

Query: 361 TDVLLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQE 420
            DV+L+ EVL +A L D S +AAQV +KQ+V   FS L QDISD L +    +K E V+ 
Sbjct: 361 EDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQK-EAVEG 420

Query: 421 YSLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVD 480
             L++ LEAS+KAVLQG+ ++  DFRQLL++++G+ I  +D I  W+Q+G QDFFR+L  
Sbjct: 421 ELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEA 480

Query: 481 RFMLLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSG 540
           +F++LSGK SS +      E   ++K+ AGL+LVLAQ+S             EIAASFSG
Sbjct: 481 QFLVLSGKTSSSNDI----EGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSG 540

Query: 541 GGTRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPR 600
           G ++ +E GPAF+P E+CR+F AA EK L  YI+ R+Q++SVLL KRF+TPNWVKHKEPR
Sbjct: 541 GNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPR 600

Query: 601 EVHMFVDLFLQELEAIGSEVKQILPQGT-RKHRRTDSNGS--TTSSRSNPLREEKLNRSN 660
           EVHM+VD+FL ELE +G EVKQ+LPQGT RKH+RTDSNGS  TTSSRSN L  +K+ RSN
Sbjct: 601 EVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSN 660

Query: 661 TQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGF 720
           +QRARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+L E+VRLQTFNRSGF
Sbjct: 661 SQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGF 720

Query: 721 QQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAK 767
           QQ+QLD+QFL+ PLKE  +DEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK
Sbjct: 721 QQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAK 774

BLAST of Carg24083 vs. ExPASy Swiss-Prot
Match: Q54KG3 (Vacuolar protein sorting-associated protein 51 homolog OS=Dictyostelium discoideum OX=44689 GN=vps51 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.0e-64
Identity = 220/797 (27.60%), Postives = 386/797 (48.43%), Query Frame = 0

Query: 11  KTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSILVQKSNLEGL 70
           ++KR+R+LL ++Y P     GS   S N    PL  I+  SFN + Y   +V+ S L  L
Sbjct: 147 ESKRVRNLLKTYYGPGIG-EGSDSSSMN---DPLN-IDGPSFNLNSYFDSIVKSSTLNQL 206

Query: 71  LQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSV 130
           +QK  +M +EI+ LD D++ LVY+NY KFI+ATD IK+M  N+  ME  M  L + +  +
Sbjct: 207 IQKDNQMVSEIRTLDGDMKTLVYDNYTKFINATDIIKKMKTNVENMEEGMALLSKNMDLI 266

Query: 131 QSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGA 190
            + S+ +N++L  +R+ I++L   +   +K+QF+  LP+ L  C+  +AY  AVR+Y   
Sbjct: 267 TNCSEKINSTLSVRRDRIDQLSGLQKFFQKLQFLTALPSSLNHCLAMQAYNQAVRYYNSN 326

Query: 191 MPIFKVYGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVD 250
             I K Y    SFQ+ +   +  +  +   L E+L S S S     E+A +L  L  PV+
Sbjct: 327 SGILKQYSHIPSFQNIQNECDSIMKTMKDKLYERLSSLSTSQTDCVESAEVLMDLLEPVE 386

Query: 251 SLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASVREFAEAIRA 310
            +++K LE  +  T+ L  N E  S   +    K+ N +           + E++  I +
Sbjct: 387 LVRSKYLESRKHHTITLLENLEKKSVEHITDYIKELNAN----------FLSEYSYNITS 446

Query: 311 YRVIF------ADSDR-------QLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGII 370
           Y+ +F      +DS +       QL   ++DL  K+ +  +  +       + +    II
Sbjct: 447 YKSLFINRLDGSDSKKERQQSLLQLEDFSKDLFNKYLNIAKSKLSSFKDPIEKIMALEII 506

Query: 371 WTDVLLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHT---RKKEE 430
            +DV  LG  L+     +         V   +   F  L + I + + Q+++    +++E
Sbjct: 507 NSDVSRLGSELSS---HEKITNIINSTVHDQIDFYFDNLQKTIKEHIHQLNSTLNERRDE 566

Query: 431 GVQEYSLQLALEASKKAVLQGSMDVLLDFRQL----LEDQSGLTISQRDSIVDWVQEGFQ 490
            ++ ++LQ   +A+ KA++    D++L F  L    L  ++    S +D+I   +Q   Q
Sbjct: 567 VLEGHNLQELSDATSKAIVN---DIILLFANLKPFFLPTETQFLSSYQDTIFTKIQVKLQ 626

Query: 491 DFFRALVDRFML-------LSGKNSSFSQSQVLPEATQAEKVV-AGLVLVLAQVSEIAAS 550
            FF  LV+   L        +     FS   +L  ++        G+ LV+  +SE    
Sbjct: 627 QFFLFLVNIHFLEYLDIIATTSNREQFSGRFLLVLSSICLYFENKGITLVVQLMSEFITV 686

Query: 551 FSGGGTRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRT---PNWV 610
              G  +      +F   ++C+  R  G + L+++  + SQ++  +L K   +    NW+
Sbjct: 687 AKQGAKKELNVF-SFNAPDLCKRVRETGLQILNVFTRLSSQKLDKILKKGLESIPNNNWL 746

Query: 611 KHKEPREVHMFVDLFLQELEAIGSEVKQILP------QGTRKHRRTDSNGSTTSSRSNPL 670
             KEPR+V    D++L+E+    +E  ++LP         + H RT S G++ SS ++  
Sbjct: 747 VLKEPRDVRSVNDIYLEEILKFQNETSKLLPVINHHHHSLKSHSRTGSGGNSVSSNNSSS 806

Query: 671 REEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVR 730
                +R N   + S         LF++K++    V+F   SV+   +KLSLK+  E +R
Sbjct: 807 NTPDGHRRNPSASSSSSSNNTNTTLFEKKVD---HVDFNTLSVLIAIIKLSLKSFNESLR 866

Query: 731 LQTFNRSGFQQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILD 770
           L+TF  +G  Q+Q+D+ +L+  L ++     + D LL E     +ERC+D +PL   I+ 
Sbjct: 867 LKTFGTNGCHQIQIDLHYLKLSLYDLFGSN-SFDNLLQECENTVTERCVDPLPLAKSIVS 917

BLAST of Carg24083 vs. ExPASy Swiss-Prot
Match: Q4V9Y0 (Vacuolar protein sorting-associated protein 51 homolog OS=Xenopus tropicalis OX=8364 GN=vps51 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 4.7e-58
Identity = 202/757 (26.68%), Postives = 357/757 (47.16%), Query Frame = 0

Query: 39  RYASPLEAINTTSFNPDQYMSILVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNK 98
           R+  P + ++   FNP+ Y++ L ++S+L  L+     M  +I++LD+++Q LVYENYNK
Sbjct: 37  RHLEPTD-VDGVHFNPELYLTKLRKESSLSQLMDVEADMVRQIRSLDSEMQTLVYENYNK 96

Query: 99  FISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL 158
           FISATDTI++M N+   ME  M+ L   +  +   S  ++++L E+ + I KL     LL
Sbjct: 97  FISATDTIRKMKNDFKKMEDEMDGLATNMAVITEFSARISSTLQERHQQITKLSGVHTLL 156

Query: 159 RKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKVYGD-SSFQDCKRASEEAIAIVL 218
           RK+QF+++LPARL KCI+  AYA AV +++ A  +   Y    SF   +   +  +A + 
Sbjct: 157 RKLQFLFELPARLKKCIELGAYAQAVSYHSKARSVLHQYQHMPSFHGIQTDCQAIMAGLA 216

Query: 219 KNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLLE----KLEQSTLDLQLNAEDL 278
             L+++    + S Q  +E   +L  L+ P   L  + L     +L     DLQ + + L
Sbjct: 217 DTLRQRFRDPASSPQDLSECVEMLLNLEEPAHLLCDEFLAHGRGRLASHLSDLQESGDIL 276

Query: 279 -----------SSALVIGSSKDGNFSESVYGASHEASVREFAEAIRAYRVIFADSDRQLI 338
                      S A ++ +S    FS+         S  + AEA             +L 
Sbjct: 277 EFVDRGCGGFISDACLLAASYQSLFSKEA------GSTAQMAEA-------------KLT 336

Query: 339 KLAQDLVTKHFDAIEQFIRKQICAAD---LLRVFGIIWTDVLLLGEVLNDAGL----PDY 398
              ++L T +F+ +E+ +R++    D   L+R        +    +++   G      + 
Sbjct: 337 SFLEELSTGYFELVEKRLRQEKSLGDNSLLVRALDRFHRRLQAPSKLVPGCGFNRRGTEI 396

Query: 399 SLKAAQVAVKQYVTCT---FSRLLQDISDALAQVHTRKKEE---GVQEYSLQLALEASKK 458
            ++AAQ  + QY+      F   L D+  ALA      KE    G     L  ++    K
Sbjct: 397 VVRAAQERLAQYLQALKDFFQGCLTDVRQALAAPRLPGKEMPALGDLLAGLSASVLNQIK 456

Query: 459 AVLQG-----SMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFMLLSG 518
            VL       + DV    +   + +      +   IV +++   Q        +F  + G
Sbjct: 457 TVLAAVHLFTAKDVAFSDKPYFKGEFCSQGVREGLIVAFIKSVCQ-----TARQFCEIPG 516

Query: 519 KNSSFSQSQVLPEATQA----EKVVAGLVLVLAQVSEIAASFSGGGTRGYEYGPAFVPAE 578
           +  + +   +L   ++     E      +L L     +          G ++ P    + 
Sbjct: 517 EKGTSTPPALLLLLSRLCLDYETSTISYILTLTDEQFL----------GQDHSPVTPVSS 576

Query: 579 ICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAI 638
           +C + R+  +  L+ Y+  +   +S +L K   T +WV   EPR V   +   ++++  +
Sbjct: 577 LCSLARSTAQTLLNQYVKSQGLVVSQMLRKSVETRDWVTTIEPRNVRAVMKRVVEDITGV 636

Query: 639 GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQL-LETHLAKLFK 698
             +V  +  +G RK   +DS+  T S  S+   + +  +S T  A     L +++ KLF 
Sbjct: 637 DVQVGLLYEEGVRKAHSSDSSKRTFSVYSSSRLQGRYAQSYTPSAPMDTNLLSNIQKLFS 696

Query: 699 QKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIA 757
           ++I+IF+ V+F + S++T  +K+SLKT LE VRL+TF R G QQ+Q+D  +L+  L    
Sbjct: 697 ERIDIFSTVQFNKVSILTGIIKISLKTFLECVRLRTFGRYGLQQIQVDCHYLQLYLWRFV 756

BLAST of Carg24083 vs. ExPASy Swiss-Prot
Match: Q3UVL4 (Vacuolar protein sorting-associated protein 51 homolog OS=Mus musculus OX=10090 GN=Vps51 PE=1 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 1.2e-56
Identity = 209/780 (26.79%), Postives = 364/780 (46.67%), Query Frame = 0

Query: 10  EKTKRMRDLLSSFYS-PDASTSGSSMGSSNRYASPLEA--INTTSFNPDQYMSILVQKSN 69
           E+ ++   +L  +Y   +   +G   G       PL+   +N   F+P+ Y+  L ++  
Sbjct: 28  ERRRKAHGMLKLYYGLSEGEVAGHPAG-----PDPLDPTDLNGAHFDPEVYLDKLRRECP 87

Query: 70  LEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEK 129
           L  L+     M  +I+ LD+D+Q LVYENYNKFISATDTI++M N+   ME  M++L   
Sbjct: 88  LAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATN 147

Query: 130 ILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRF 189
           +  + + S  ++ +L ++ E I KL     LLRK+QF+++LP+RL KC++  AY  AVR+
Sbjct: 148 MAVITNFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRY 207

Query: 190 YTGAMPIFKVYGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLD 249
              A  + + Y    SF+  +   +   A + + L+++          +AE   LL  L 
Sbjct: 208 QGRARAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGCSGAPEQAECVELLLALG 267

Query: 250 FPVDSLKAKLLE----KLEQ--STLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEAS 309
            P + L  + L     +LE+  S+L+ +L     +  ++  + + GN             
Sbjct: 268 EPAEELCEEFLAHARGRLEEELSSLEAELGPSPPAPDVLEFTDRGGN-----------GF 327

Query: 310 VREFAEAIRAYRVIFADSD----RQLIKLAQDLVTKHFDAIEQFIRKQICAAD---LLRV 369
           V    +   AY+ +FA        +L   AQ+L  ++F  +E+ + ++   +D   L+R 
Sbjct: 328 VGGLCQVAAAYQELFAAQGPAGAEKLAAFAQELGGRYFALVERRLAQEQGGSDNSLLVRA 387

Query: 370 FGIIWTDVLLLGEVLNDAGLPDYSLKAAQVAVK-------QYVTCTFSRLLQDISDALAQ 429
                  +   G +L  AGL + + +  +   +       Q +   F   L D+  ALA 
Sbjct: 388 LDRFHRRLRAPGALLAAAGLSESATEIVERVARERLSHHLQGLKAAFLSSLTDVRQALAA 447

Query: 430 VHTRKKEEGVQEYSLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQ 489
                K EG     L   + +S  + ++ S+  +  F       S     + +     V+
Sbjct: 448 PRLAGK-EGPSLAELLANVASSILSHIKTSLASVHLFTAKEVSFSNKPYFRGEFCSQGVR 507

Query: 490 EG-FQDFFRAL---VDRFMLLSGKNSSFSQSQVLPEATQA----EKVVAGLVLVLAQVSE 549
           EG    F R++      F    G+    +   +L   ++     E      +L L     
Sbjct: 508 EGLIVGFIRSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILTLTDEQF 567

Query: 550 IAASFSGGGTRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNW 609
           +            +  P    + +C   R    + L  Y+ ++   IS +L K   T +W
Sbjct: 568 LV----------QDQSPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRDW 627

Query: 610 VKHKEPREVHMFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKL 669
           +   EPR V   +   +++  AI  +V  +  +G RK + +DS+  T S  S+  ++ + 
Sbjct: 628 LSTLEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSSSRQQGRY 687

Query: 670 NRSNTQRARSQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTF 729
             S T  A     L +++ KLF ++I++F+ VEF + SV+T  +K+SLKTLLE VRL+TF
Sbjct: 688 APSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLECVRLRTF 747

Query: 730 NRSGFQQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQ 757
            R G QQVQ+D  FL+  L     DE  +  LLDEV+ +A+ RC D +P+EP +++ + +
Sbjct: 748 GRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPSVVEVICE 780

BLAST of Carg24083 vs. ExPASy Swiss-Prot
Match: Q9UID3 (Vacuolar protein sorting-associated protein 51 homolog OS=Homo sapiens OX=9606 GN=VPS51 PE=1 SV=2)

HSP 1 Score: 222.6 bits (566), Expect = 1.5e-56
Identity = 215/787 (27.32%), Postives = 362/787 (46.00%), Query Frame = 0

Query: 6   GSMDEKTKRMRDLLSSFYS-PDASTSGSSMGSSNRYASPLEA--INTTSFNPDQYMSILV 65
           G   E+ ++   +L  +Y   +   +G   G       PL+   +N   F+P+ Y+  L 
Sbjct: 24  GEAPERRRKAHGMLKLYYGLSEGEAAGRPAG-----PDPLDPTDLNGAHFDPEVYLDKLR 83

Query: 66  QKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQ 125
           ++  L  L+     M  +I+ LD+D+Q LVYENYNKFISATDTI++M N+   ME  M++
Sbjct: 84  RECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDR 143

Query: 126 LLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYAD 185
           L   +  +   S  ++ +L ++ E I KL     LLRK+QF+++LP+RL KC++  AY  
Sbjct: 144 LATNMAVITDFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQ 203

Query: 186 AVRFYTGAMPIFKVYGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLL 245
           AVR+   A  + + Y    SF+  +   +   A + + L+++          +AE   LL
Sbjct: 204 AVRYQGRAQAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGGSGAPEQAECVELL 263

Query: 246 KQLDFPVDSLKAKLLE----KLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHE 305
             L  P + L  + L     +LE+   +L+        A  +    D   S  V G    
Sbjct: 264 LALGEPAEELCEEFLAHARGRLEKELRNLEAELGPSPPAPDVLEFTDHGGSGFVGGLCQV 323

Query: 306 ASVREFAEAIRAYRVIFADSD----RQLIKLAQDLVTKHFDAIEQFIRKQICAAD---LL 365
           A+         AY+ +FA        +L   A+ L +++F  +E+ + ++    D   L+
Sbjct: 324 AA---------AYQELFAAQGPAGAEKLAAFARQLGSRYFALVERRLAQEQGGGDNSLLV 383

Query: 366 RVFGIIWTDVLLLGEVLNDAGLPDYSLKAAQVAVK-------QYVTCTFSRLLQDISDAL 425
           R        +   G +L  AGL D + +  +   +       Q +   F   L D+  AL
Sbjct: 384 RALDRFHRRLRAPGALLAAAGLADAATEIVERVARERLGHHLQGLRAAFLGCLTDVRQAL 443

Query: 426 AQVHTRKKE-EGVQEYSLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVD 485
           A      KE  G+ E    +A  +S  + ++ S+  +  F       S     + +    
Sbjct: 444 AAPRVAGKEGPGLAELLANVA--SSILSHIKASLAAVHLFTAKEVSFSNKPYFRGEFCSQ 503

Query: 486 WVQEGFQDFFRALVDRFMLLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSEIAASF 545
            V+EG             L+ G   S  Q+      +  EK  A    +L  +S +   +
Sbjct: 504 GVREG-------------LIVGFVHSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDY 563

Query: 546 SGGGTRGY------------EYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTK 605
               T  Y            +  P    + +C   R    + L  Y+ ++   IS +L K
Sbjct: 564 E-TATISYILTLTDEQFLVQDQFPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRK 623

Query: 606 RFRTPNWVKHKEPREVHMFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSN 665
              T +W+   EPR V   +   +++  AI  +V  +  +G RK + +DS+  T S  S+
Sbjct: 624 SVETRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSS 683

Query: 666 PLREEKLNRSNTQRARSQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLE 725
             ++ +   S T  A     L +++ KLF ++I++F+ VEF + SV+T  +K+SLKTLLE
Sbjct: 684 SRQQGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLE 743

Query: 726 FVRLQTFNRSGFQQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPP 757
            VRL+TF R G QQVQ+D  FL+  L     DE  +  LLDEV+ +A+ RC D +P+EP 
Sbjct: 744 CVRLRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPS 780

BLAST of Carg24083 vs. ExPASy TrEMBL
Match: A0A6J1EMM4 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111435972 PE=3 SV=1)

HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 763/783 (97.45%), Postives = 765/783 (97.70%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNG MDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGN SESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDA EQFIRKQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS             EIAASFSGGGT
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQNP 780

BLAST of Carg24083 vs. ExPASy TrEMBL
Match: A0A6J1JAN2 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483261 PE=3 SV=1)

HSP 1 Score: 1425.6 bits (3689), Expect = 0.0e+00
Identity = 759/783 (96.93%), Postives = 766/783 (97.83%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGN SESVYGASHE SV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEGSV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHF+AIEQFI+KQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFNAIEQFIKKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTC FSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCMFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKNSS+SQSQVLPEATQAEKVVAGLVLVLAQ+S             EIAASFSGGGT
Sbjct: 481 LLSGKNSSYSQSQVLPEATQAEKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEF+RLQTFNRSGFQQVQLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFIRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLI+AKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIKAKLAKAKDQNP 780

BLAST of Carg24083 vs. ExPASy TrEMBL
Match: A0A6J1EU93 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111435972 PE=3 SV=1)

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 748/783 (95.53%), Postives = 750/783 (95.79%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNG MDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGN SESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDA EQFIRKQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALE               DFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALE---------------DFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS             EIAASFSGGGT
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQNP 768

BLAST of Carg24083 vs. ExPASy TrEMBL
Match: A0A6J1J462 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483261 PE=3 SV=1)

HSP 1 Score: 1390.2 bits (3597), Expect = 0.0e+00
Identity = 744/783 (95.02%), Postives = 751/783 (95.91%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQKHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGN SESVYGASHE SV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEGSV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHF+AIEQFI+KQICAADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFNAIEQFIKKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGLPDYSLKAAQVAVKQYVTC FSRLLQDISDALAQVHTRKKEEGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCMFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QLALE               DFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALE---------------DFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKNSS+SQSQVLPEATQAEKVVAGLVLVLAQ+S             EIAASFSGGGT
Sbjct: 481 LLSGKNSSYSQSQVLPEATQAEKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEF+RLQTFNRSGFQQVQLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFIRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLI+AKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIKAKLAKAKDQNP 768

BLAST of Carg24083 vs. ExPASy TrEMBL
Match: A0A1S3CES5 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucumis melo OX=3656 GN=LOC103500085 PE=3 SV=1)

HSP 1 Score: 1347.0 bits (3485), Expect = 0.0e+00
Identity = 719/783 (91.83%), Postives = 740/783 (94.51%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60
           MEID+  MDEK KRMRDLLSSFYSPDAS S S  GSSNRYASPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+SALV  SSKDGN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300

Query: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360
           REF EA+RAYRVIFADSDRQLIKLAQDLVTKHFD+ EQFI+KQICAADLL VFG IWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360

Query: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420
           LLLGEVLNDAGL DYSLKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420

Query: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480
           QL LEASKKAVLQGSMD+LLDFRQLLEDQSGL I+QRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSGGGT 540
           LLSGKN+S++QSQ L EATQAEKV AGLVLVLAQ+S             EIAASFSGGG 
Sbjct: 481 LLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 771
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD+IPLEPPILDKLIQAKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNP 780

BLAST of Carg24083 vs. TAIR 10
Match: AT4G02030.1 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 952.2 bits (2460), Expect = 2.6e-277
Identity = 520/785 (66.24%), Postives = 624/785 (79.49%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPD--ASTSGSSMGSSNRYASPLEAINTTSFNPDQYM 60
           M  +   MDEK KRMRDLLSSFY+PD   STSGSS+ +S       + IN+TSF+ DQYM
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSINAS------FDNINSTSFDADQYM 60

Query: 61  SILVQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMET 120
            ++++KSNLE LLQ+HV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME 
Sbjct: 61  DLMIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEG 120

Query: 121 NMEQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTE 180
           NM+QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+E
Sbjct: 121 NMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSE 180

Query: 181 AYADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAA 240
           AY DAVRFYTGAMPI KVYGD+SFQDC+RASEEAI I++KNLQ KLFSDSESIQ RAEAA
Sbjct: 181 AYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAA 240

Query: 241 VLLKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNFSESVYGAS-HE 300
           VLLKQLD PVDSLKAKLLEKLEQS   LQ+  E+ S+ +    S +   S   + A  HE
Sbjct: 241 VLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHE 300

Query: 301 ASVREFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIW 360
            +VR F+EAIRAYR IF DS+ +L KLA+ L   HF+ +E +I+K++ AAD L +F I+W
Sbjct: 301 DAVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVW 360

Query: 361 TDVLLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQE 420
            DV+L+ EVL +A L D S +AAQV +KQ+V   FS L QDISD L +    +K E V+ 
Sbjct: 361 EDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQK-EAVEG 420

Query: 421 YSLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVD 480
             L++ LEAS+KAVLQG+ ++  DFRQLL++++G+ I  +D I  W+Q+G QDFFR+L  
Sbjct: 421 ELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEA 480

Query: 481 RFMLLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVS-------------EIAASFSG 540
           +F++LSGK SS +      E   ++K+ AGL+LVLAQ+S             EIAASFSG
Sbjct: 481 QFLVLSGKTSSSNDI----EGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSG 540

Query: 541 GGTRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPR 600
           G ++ +E GPAF+P E+CR+F AA EK L  YI+ R+Q++SVLL KRF+TPNWVKHKEPR
Sbjct: 541 GNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPR 600

Query: 601 EVHMFVDLFLQELEAIGSEVKQILPQGT-RKHRRTDSNGS--TTSSRSNPLREEKLNRSN 660
           EVHM+VD+FL ELE +G EVKQ+LPQGT RKH+RTDSNGS  TTSSRSN L  +K+ RSN
Sbjct: 601 EVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSN 660

Query: 661 TQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGF 720
           +QRARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+L E+VRLQTFNRSGF
Sbjct: 661 SQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGF 720

Query: 721 QQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAK 767
           QQ+QLD+QFL+ PLKE  +DEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK
Sbjct: 721 QQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAK 774

BLAST of Carg24083 vs. TAIR 10
Match: AT4G02030.2 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 938.3 bits (2424), Expect = 3.8e-273
Identity = 520/810 (64.20%), Postives = 624/810 (77.04%), Query Frame = 0

Query: 1   MEIDNGSMDEKTKRMRDLLSSFYSPD--ASTSGSSMGSSNRYASPLEAINTTSFNPDQYM 60
           M  +   MDEK KRMRDLLSSFY+PD   STSGSS+ +S       + IN+TSF+ DQYM
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSINAS------FDNINSTSFDADQYM 60

Query: 61  SIL-------------------------VQKSNLEGLLQKHVKMAAEIKNLDTDLQMLVY 120
            ++                         ++KSNLE LLQ+HV+MAAEIKNLDTDLQMLVY
Sbjct: 61  DLMIRLFDLGLSDLPAMVDSSELNWVYEIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVY 120

Query: 121 ENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDGVNTSLFEKREHIEKLHR 180
           ENYNKFISATDTIKRM +NI GME NM+QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHR
Sbjct: 121 ENYNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHR 180

Query: 181 TRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAI 240
           TRNLLRKVQFIYDLPARL KCIK+EAY DAVRFYTGAMPI KVYGD+SFQDC+RASEEAI
Sbjct: 181 TRNLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAI 240

Query: 241 AIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDL 300
            I++KNLQ KLFSDSESIQ RAEAAVLLKQLD PVDSLKAKLLEKLEQS   LQ+  E+ 
Sbjct: 241 EIIIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEA 300

Query: 301 SSALVIGSSKDGNFSESVYGAS-HEASVREFAEAIRAYRVIFADSDRQLIKLAQDLVTKH 360
           S+ +    S +   S   + A  HE +VR F+EAIRAYR IF DS+ +L KLA+ L   H
Sbjct: 301 STLVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFPDSEERLFKLARALTAMH 360

Query: 361 FDAIEQFIRKQICAADLLRVFGIIWTDVLLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTF 420
           F+ +E +I+K++ AAD L +F I+W DV+L+ EVL +A L D S +AAQV +KQ+V   F
Sbjct: 361 FEYMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMF 420

Query: 421 SRLLQDISDALAQVHTRKKEEGVQEYSLQLALEASKKAVLQGSMDVLLDFRQLLEDQSGL 480
           S L QDISD L +    +K E V+   L++ LEAS+KAVLQG+ ++  DFRQLL++++G+
Sbjct: 421 SHLQQDISDTLLKFDINQK-EAVEGELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGI 480

Query: 481 TISQRDSIVDWVQEGFQDFFRALVDRFMLLSGKNSSFSQSQVLPEATQAEKVVAGLVLVL 540
            I  +D I  W+Q+G QDFFR+L  +F++LSGK SS +      E   ++K+ AGL+LVL
Sbjct: 481 FIKMKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSNDI----EGKSSDKIHAGLILVL 540

Query: 541 AQVS-------------EIAASFSGGGTRGYEYGPAFVPAEICRMFRAAGEKFLHLYINM 600
           AQ+S             EIAASFSGG ++ +E GPAF+P E+CR+F AA EK L  YI+ 
Sbjct: 541 AQLSVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDT 600

Query: 601 RSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAIGSEVKQILPQGT-RKHRRT 660
           R+Q++SVLL KRF+TPNWVKHKEPREVHM+VD+FL ELE +G EVKQ+LPQGT RKH+RT
Sbjct: 601 RTQKVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRT 660

Query: 661 DSNGS--TTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVV 720
           DSNGS  TTSSRSN L  +K+ RSN+QRARSQL ETHLAKLFKQK+EIFT+VEFTQ SVV
Sbjct: 661 DSNGSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVV 720

Query: 721 TTAVKLSLKTLLEFVRLQTFNRSGFQQVQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAA 767
           TT VKL LK+L E+VRLQTFNRSGFQQ+QLD+QFL+ PLKE  +DEAAIDFLLDEVIVAA
Sbjct: 721 TTTVKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAA 780