Carg23727 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23727
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Ubiquitin-conjugating enzyme E2 variant 1D
Location	Carg_Chr15: 6085513 .. 6087817 (-)
RNA-Seq Expression	Carg23727
Synteny	Carg23727

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGACGAGGCCGCGAAGGTTGTTGGTGAGTGTTTGGAGCCCCAGTTTTTCCAATTCCACAATTCGACATTTCCTATTTTGTTAGCCTTTTTTTGTTCTTTTTACTGTCAACAACGATTTTAAACTTCTTCGGGATATTGGAGTTTGTTCTAGTTTTGAATCAACTTTTGTTATGATTTTTTCTTATACTATAGAGTGGAAATTGCAAGCTCTGGCGTTAATGTGTGGAAATTTCGTTAGTGAATTCAGATTTTGTTTTATTTAGCTAACTTTGGAATGTTGTACTCCGGAGATGCTTGGATTTTTTTGTTTACTCCATATTGGAAGGTAGTGTGGTGATATGGATAGATGGTTCTATTCTCGTTTCTTTTTCTGATTCGATCAGTTTGGGTCTGCAGGTTGGGCTCTACTAGCTGTTGGTTAAATTCTTGGCGTTTTACTTTTGGCTTTATGTTATGTTGTCTGGATTACCAGACTGTTTGAATGGAGAATCTGTTCGTTTGATTTCCATTCCTATCTTCATGTTAACGAAAGTTGGGCTTCTGACTAAAAAATAAATGTAATGTTATTTCTTCAACAATCTGTAATTTATAGAAAAAGTGTAAAGAAACCCGCAAAAGATTGGGCCCGCCTAATGTAAGTTCATTTTTAGCACCATAAGCTCTAATTGGAACAATATTCTATTTTTTTTTCAGTTCCTCGAAACTTCAGATTACTGGAAGAACTTGAAAGAGGTGAAAAAGGAATCGGGGATGGAACTGTCAGTTATGGGATGGATGATGCTGATGATATCTGTATGGCTTCTTGGACGGGAACTATTATTGGGCCGCCTAATGTAAGTTCATTTTTCATCCTTTTGGTTCCTCTTCTTGTGGAGTCTCTGATGGTATTTGGTTCTGCTTTATAAATGTTGATATGGATAGTTACAGACAGCTATGGAAATATCAATTTCTGCACTGTATCAAGTGTAGGATTTCACATAAATGTTTACAAGAAAATTTGTTATCATCAGAATTTAGATAATTGAATTCGAATTGTAGATAACAATGTTTCACAACAATTTGCTTCTCGTATCGACAAATTAGATGCTTTTAGTTTAGGGGTGCGCAGGTTTGGAACCCTATACAAACAAGAGGCTAGCGCGCAATGACTTTCTCTGATAGTGACCGCTCACACTAGTTCGACCTATAGAAAATATCATTTTGTCACAAATACAGGCCGGAATTCCACTAAACCTTGTCCACAGCAGACACTATTTTTGTAGTATAACTTGGACACTTTGACTGGTATTACTAGGGAATACATTTGGAATTAGTTTTGATGTAAAATATTTAAATTGTAGATGATATTGAGAGATTCTGGTTCTTCTTTCATTACTAGTTCCTTTTTTTTTTCTTTTTTTTTTTTCCCTTTTAATCTTCTCGTGTTTATTTTATCTCTCTTTGTTTTCTGATGTTCTCTTGGTACCTTTTGGTTTGGGCCTTATTTTTTGTATGCCGTTTTAATTAATTTGAGAAAAACAAGTCGTTCTCTTCGTTAAAAAGATGTCAATGTCAGACTACTAGGTACATCATTTGAGAATAAGCAACCAGTGTGATAATCTAGCTTATGTGCTTAGACTACAGATTGACCGCAAATCAAGAAAAACCCTATGCCATGTTTATCATCTTTAAATTTTAATATGATTATGAAGATTTGAGCATGTAGTTTTCGGACACTCCAGCCATTTCCGTGCTAAAGGATTGAAATTCAGGAGTTGAAGTATACAAGATCCTGATTTCCTGATTGTATTTAACTTGAAAATTCACAATGAAAGAAAACTGGTGTTTTTGATAACCTCTCATGTTGATAAAAATGATGGCTTCAGCGTTGTAGTGTCATTGCGTCGGTGAAAATTGATGTATTCTTCTTTATTGTTGTTGTTGGGAGGGGATGTAAGAAATTGTAAATTGAATGTTTTCTTCTTGCTTTTCAGACTGTTCATGAAGGGCGCATCCATCAACTCAAGCTATTCTGTGGGCAGGATTACCCTGATAATCCTCCGAACGTTAAATTTCAAACTCCGATTAATATGACCTGTGTCAATTCAGAAACCGGGGTGGTATGTTAGGATTTTGTGAAGCACTTTTGCTACAATGTGACTGAAACCAAATTGACCGCTCATGTGAATGTGTAGGTGGAACCAAGCCTTTTCCCCATGCTTGCTAACTGGCAAAGGGAGCATACAATGGAGGACATATTAATGCAATTGAAGAAAGAAATGACTTCTCCCCAGAACAGGAGACTAAGTCAGCCTTCTGAA

mRNA sequence

ATGGGTGACGAGGCCGCGAAGGTTGTTGTTCCTCGAAACTTCAGATTACTGGAAGAACTTGAAAGAGGTGAAAAAGGAATCGGGGATGGAACTGTCAGTTATGGGATGGATGATGCTGATGATATCTGTATGGCTTCTTGGACGGGAACTATTATTGGGCCGCCTAATACTGTTCATGAAGGGCGCATCCATCAACTCAAGCTATTCTGTGGGCAGGATTACCCTGATAATCCTCCGAACGTTAAATTTCAAACTCCGATTAATATGACCTGTGTCAATTCAGAAACCGGGGTGGTGGAACCAAGCCTTTTCCCCATGCTTGCTAACTGGCAAAGGGAGCATACAATGGAGGACATATTAATGCAATTGAAGAAAGAAATGACTTCTCCCCAGAACAGGAGACTAAGTCAGCCTTCTGAA

Coding sequence (CDS)

ATGGGTGACGAGGCCGCGAAGGTTGTTGTTCCTCGAAACTTCAGATTACTGGAAGAACTTGAAAGAGGTGAAAAAGGAATCGGGGATGGAACTGTCAGTTATGGGATGGATGATGCTGATGATATCTGTATGGCTTCTTGGACGGGAACTATTATTGGGCCGCCTAATACTGTTCATGAAGGGCGCATCCATCAACTCAAGCTATTCTGTGGGCAGGATTACCCTGATAATCCTCCGAACGTTAAATTTCAAACTCCGATTAATATGACCTGTGTCAATTCAGAAACCGGGGTGGTGGAACCAAGCCTTTTCCCCATGCTTGCTAACTGGCAAAGGGAGCATACAATGGAGGACATATTAATGCAATTGAAGAAAGAAATGACTTCTCCCCAGAACAGGAGACTAAGTCAGCCTTCTGAA

Protein sequence

MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHEGRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDILMQLKKEMTSPQNRRLSQPSE

Homology

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BLAST of Carg23727 vs. NCBI nr
Match: KAG7016785.1 (Ubiquitin-conjugating enzyme E2 variant 1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 296.2 bits (757), Expect = 1.5e-76
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. NCBI nr
Match: KAG6579270.1 (Ubiquitin-conjugating enzyme E2 variant 1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 296.2 bits (757), Expect = 1.5e-76
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. NCBI nr
Match: XP_022939181.1 (uncharacterized protein LOC111445176 [Cucurbita moschata])

HSP 1 Score: 296.2 bits (757), Expect = 1.5e-76
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE
Sbjct: 522 MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 581

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 582 GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 641

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 642 MQLKKEMTSPQNRRLSQPSE 661

[Close]

BLAST of Carg23727 vs. NCBI nr
Match: KGN51442.1 (hypothetical protein Csa_008754 [Cucumis sativus])

HSP 1 Score: 285.0 bits (728), Expect = 3.4e-73
Identity = 136/140 (97.14%), Postives = 138/140 (98.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT INMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGQDYPDNPPNVRFQTRINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. NCBI nr
Match: XP_011656243.1 (zinc finger protein VAR3, chloroplastic [Cucumis sativus])

HSP 1 Score: 285.0 bits (728), Expect = 3.4e-73
Identity = 136/140 (97.14%), Postives = 138/140 (98.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 521 MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 580

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT INMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 581 GRIYQLKLFCGQDYPDNPPNVRFQTRINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 640

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 641 MQLKKEMTSPQNRRLSQPSE 660

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BLAST of Carg23727 vs. ExPASy Swiss-Prot
Match: Q93YP0 (Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana OX=3702 GN=UEV1A PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 3.1e-61
Identity = 109/140 (77.86%), Postives = 124/140 (88.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           M  E AKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GPPNT +E
Sbjct: 1   MSSEEAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMQSWTGTILGPPNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG++YP++PP V+FQT INM CVN ETGVVEPSLFPML NW+RE+TMEDIL
Sbjct: 61  GKIFQLKLFCGKEYPESPPTVRFQTRINMACVNPETGVVEPSLFPMLTNWRREYTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           ++LKKEM +  NR+L+QP E
Sbjct: 121 VKLKKEMMTSHNRKLAQPPE 140

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BLAST of Carg23727 vs. ExPASy Swiss-Prot
Match: Q9CAB6 (Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana OX=3702 GN=UEV1B PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.7e-59
Identity = 107/140 (76.43%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MG E  KVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GP NT +E
Sbjct: 1   MGSEEEKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG+DYP++PP V+FQ+ INM CVN E GVV+PS FPML+NW+RE TMED+L
Sbjct: 61  GKIFQLKLFCGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           +QLKKEM S QNR+L+QP E
Sbjct: 121 IQLKKEMMSSQNRKLAQPLE 140

[Close]

BLAST of Carg23727 vs. ExPASy Swiss-Prot
Match: Q9SVD7 (Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana OX=3702 GN=UEV1D PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 2.7e-57
Identity = 103/140 (73.57%), Postives = 118/140 (84.29%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           +G   + VVVPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M SWTGTIIGP NTVHE
Sbjct: 3   LGSGGSSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHE 62

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFC +DYP+ PP V+F + +NM CVN ETGVV+P  F +LANWQRE+TMEDIL
Sbjct: 63  GRIYQLKLFCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDIL 122

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           +QLKKEM++  NR+L QP E
Sbjct: 123 VQLKKEMSTSHNRKLVQPPE 142

[Close]

BLAST of Carg23727 vs. ExPASy Swiss-Prot
Match: Q9SJ44 (Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana OX=3702 GN=UEV1C PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 4.4e-55
Identity = 101/135 (74.81%), Postives = 114/135 (84.44%), Query Frame = 0


Query: 6   AKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHEGRIHQ 65
           + VVVPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M SWTGTIIGP NTVHEGRI+Q
Sbjct: 7   SSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQ 66

Query: 66  LKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDILMQLKK 125
           LKLFC +DYP+ PP V+F + INMTCVN +TGVV+   F +LANWQR++TMEDIL QLKK
Sbjct: 67  LKLFCDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKK 126

Query: 126 EMTSPQNRRLSQPSE 141
           EM +  NR+L QP E
Sbjct: 127 EMAASHNRKLVQPPE 141

[Close]

BLAST of Carg23727 vs. ExPASy Swiss-Prot
Match: Q54D06 (Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum OX=44689 GN=ube2v PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 3.4e-39
Identity = 70/131 (53.44%), Postives = 98/131 (74.81%), Query Frame = 0


Query: 10  VPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHEGRIHQLKLF 69
           +PRNF LLEELE GEK  GDGT+SYG++  DDI ++SW GTIIGP NT  + R + LKL+
Sbjct: 4   IPRNFVLLEELESGEKSTGDGTISYGLESPDDILLSSWIGTIIGPQNTPFDNRFYSLKLY 63

Query: 70  CGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDILMQLKKEMTS 129
           C +DYP+ PP V+F T IN+ CVN + GVV+P   P+L+NW  ++ +++IL +L++EM+S
Sbjct: 64  CDKDYPNKPPTVRFTTKINIGCVNQQNGVVDPKNLPILSNWTSKNRIQNILSELRREMSS 123

Query: 130 PQNRRLSQPSE 141
             N++L QP E
Sbjct: 124 SSNKKLVQPPE 134

[Close]

BLAST of Carg23727 vs. ExPASy TrEMBL
Match: A0A6J1FF74 (uncharacterized protein LOC111445176 OS=Cucurbita moschata OX=3662 GN=LOC111445176 PE=4 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 7.2e-77
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE
Sbjct: 522 MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 581

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 582 GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 641

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 642 MQLKKEMTSPQNRRLSQPSE 661

[Close]

BLAST of Carg23727 vs. ExPASy TrEMBL
Match: A0A0A0KSP5 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G547590 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.7e-73
Identity = 136/140 (97.14%), Postives = 138/140 (98.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT INMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGQDYPDNPPNVRFQTRINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. ExPASy TrEMBL
Match: A0A5D3C2X2 (Zinc finger protein VAR3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3042G00070 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 8.2e-73
Identity = 134/140 (95.71%), Postives = 137/140 (97.86%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT +NMTCVN ETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGQDYPDNPPNVRFQTRVNMTCVNPETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. ExPASy TrEMBL
Match: A0A5A7TU26 (Zinc finger protein VAR3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G00820 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 8.2e-73
Identity = 134/140 (95.71%), Postives = 137/140 (97.86%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT +NMTCVN ETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGQDYPDNPPNVRFQTRVNMTCVNPETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. ExPASy TrEMBL
Match: A0A1S4E671 (uncharacterized protein LOC103483035 OS=Cucumis melo OX=3656 GN=LOC103483035 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 8.2e-73
Identity = 134/140 (95.71%), Postives = 137/140 (97.86%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 565 MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 624

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT +NMTCVN ETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 625 GRIYQLKLFCGQDYPDNPPNVRFQTRVNMTCVNPETGVVEPSLFPMLANWQREHTMEDIL 684

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 685 MQLKKEMTSPQNRRLSQPSE 704

[Close]

BLAST of Carg23727 vs. TAIR 10
Match: AT1G23260.1 (MMS ZWEI homologue 1 )

HSP 1 Score: 235.7 bits (600), Expect = 2.2e-62
Identity = 109/140 (77.86%), Postives = 124/140 (88.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           M  E AKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GPPNT +E
Sbjct: 1   MSSEEAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMQSWTGTILGPPNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG++YP++PP V+FQT INM CVN ETGVVEPSLFPML NW+RE+TMEDIL
Sbjct: 61  GKIFQLKLFCGKEYPESPPTVRFQTRINMACVNPETGVVEPSLFPMLTNWRREYTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           ++LKKEM +  NR+L+QP E
Sbjct: 121 VKLKKEMMTSHNRKLAQPPE 140

[Close]

BLAST of Carg23727 vs. TAIR 10
Match: AT1G70660.1 (MMS ZWEI homologue 2 )

HSP 1 Score: 229.9 bits (585), Expect = 1.2e-60
Identity = 107/140 (76.43%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MG E  KVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GP NT +E
Sbjct: 1   MGSEEEKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG+DYP++PP V+FQ+ INM CVN E GVV+PS FPML+NW+RE TMED+L
Sbjct: 61  GKIFQLKLFCGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           +QLKKEM S QNR+L+QP E
Sbjct: 121 IQLKKEMMSSQNRKLAQPLE 140

[Close]

BLAST of Carg23727 vs. TAIR 10
Match: AT1G70660.2 (MMS ZWEI homologue 2 )

HSP 1 Score: 229.9 bits (585), Expect = 1.2e-60
Identity = 107/140 (76.43%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MG E  KVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GP NT +E
Sbjct: 1   MGSEEEKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG+DYP++PP V+FQ+ INM CVN E GVV+PS FPML+NW+RE TMED+L
Sbjct: 61  GKIFQLKLFCGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           +QLKKEM S QNR+L+QP E
Sbjct: 121 IQLKKEMMSSQNRKLAQPLE 140

[Close]

BLAST of Carg23727 vs. TAIR 10
Match: AT3G52560.1 (ubiquitin E2 variant 1D-4 )

HSP 1 Score: 222.6 bits (566), Expect = 1.9e-58
Identity = 103/140 (73.57%), Postives = 118/140 (84.29%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           +G   + VVVPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M SWTGTIIGP NTVHE
Sbjct: 3   LGSGGSSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHE 62

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFC +DYP+ PP V+F + +NM CVN ETGVV+P  F +LANWQRE+TMEDIL
Sbjct: 63  GRIYQLKLFCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDIL 122

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           +QLKKEM++  NR+L QP E
Sbjct: 123 VQLKKEMSTSHNRKLVQPPE 142

[Close]

BLAST of Carg23727 vs. TAIR 10
Match: AT1G70650.2 (Ran BP2/NZF zinc finger-like superfamily protein )

HSP 1 Score: 220.3 bits (560), Expect = 9.6e-58
Identity = 101/134 (75.37%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 7   KVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHEGRIHQL 66
           +++VPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GP NT +EG+I QL
Sbjct: 443 ELLVPRNFRLLEELERGEKGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYEGKIFQL 502

Query: 67  KLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDILMQLKKE 126
           KLFCG+DYP++PP V+FQ+ INM CVN E GVV+PS FPML+NW+RE TMED+L+QLKKE
Sbjct: 503 KLFCGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLLIQLKKE 562

Query: 127 MTSPQNRRLSQPSE 141
           M S QNR+L+QP E
Sbjct: 563 MMSSQNRKLAQPLE 576

The following BLAST results are available for this feature:

BLAST of Carg23727 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016785.1	1.5e-76	100.00	Ubiquitin-conjugating enzyme E2 variant 1A, partial [Cucurbita argyrosperma subs...	[more]
KAG6579270.1	1.5e-76	100.00	Ubiquitin-conjugating enzyme E2 variant 1B, partial [Cucurbita argyrosperma subs...	[more]
XP_022939181.1	1.5e-76	100.00	uncharacterized protein LOC111445176 [Cucurbita moschata]	[more]
KGN51442.1	3.4e-73	97.14	hypothetical protein Csa_008754 [Cucumis sativus]	[more]
XP_011656243.1	3.4e-73	97.14	zinc finger protein VAR3, chloroplastic [Cucumis sativus]	[more]

BLAST of Carg23727 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93YP0	3.1e-61	77.86	Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana OX=3702 GN=UE...	[more]
Q9CAB6	1.7e-59	76.43	Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana OX=3702 GN=UE...	[more]
Q9SVD7	2.7e-57	73.57	Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana OX=3702 GN=UE...	[more]
Q9SJ44	4.4e-55	74.81	Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana OX=3702 GN=UE...	[more]
Q54D06	3.4e-39	53.44	Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum OX=...	[more]

BLAST of Carg23727 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FF74	7.2e-77	100.00	uncharacterized protein LOC111445176 OS=Cucurbita moschata OX=3662 GN=LOC1114451...	[more]
A0A0A0KSP5	1.7e-73	97.14	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G547590 PE...	[more]
A0A5D3C2X2	8.2e-73	95.71	Zinc finger protein VAR3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7TU26	8.2e-73	95.71	Zinc finger protein VAR3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A1S4E671	8.2e-73	95.71	uncharacterized protein LOC103483035 OS=Cucumis melo OX=3656 GN=LOC103483035 PE=...	[more]

BLAST of Carg23727 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23260.1	2.2e-62	77.86	MMS ZWEI homologue 1	[more]
AT1G70660.1	1.2e-60	76.43	MMS ZWEI homologue 2	[more]
AT1G70660.2	1.2e-60	76.43	MMS ZWEI homologue 2	[more]
AT3G52560.1	1.9e-58	73.57	ubiquitin E2 variant 1D-4	[more]
AT1G70650.2	9.6e-58	75.37	Ran BP2/NZF zinc finger-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 14..140 e-value: 3.2E-6 score: 36.6
None	No IPR available	PANTHER	PTHR24068:SF344	UBIQUITIN-CONJUGATING ENZYME E2 VARIANT 1A-LIKE	coord: 8..137
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 8..137
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 2..140 e-value: 6.3E-42 score: 144.6
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 9..138
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 41..133 e-value: 1.9E-15 score: 56.7
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 11..140 score: 34.652679

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23727-RA	Carg23727-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006301	postreplication repair
biological_process	GO:0070534	protein K63-linked ubiquitination
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding