Carg23720 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23720
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Carg_Chr15: 5972300 .. 5972926 (-)
RNA-Seq Expression	Carg23720
Synteny	Carg23720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCGTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCTTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCGGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTGTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

mRNA sequence

ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCGTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCTTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCGGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTGTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

Coding sequence (CDS)

ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCGTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGCTTCATTACTCTCTATCCCGACGTCTGCTACACTTCGCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCGGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTGTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

Protein sequence

MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRIP

Homology

[Close]

BLAST of Carg23720 vs. NCBI nr
Match: KAG7016778.1 (hypothetical protein SDJN02_21888, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 412.1 bits (1058), Expect = 2.8e-111
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL
Sbjct: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of Carg23720 vs. NCBI nr
Match: KAG6579262.1 (hypothetical protein SDJN03_23710, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 408.3 bits (1048), Expect = 4.0e-110
Identity = 206/208 (99.04%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 33  MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 92

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 93  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 152

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 153 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 212

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 213 VCAKAEKVKKHTSNALALVNSFVEKRIP 240

[Close]

BLAST of Carg23720 vs. NCBI nr
Match: XP_022938421.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 408.3 bits (1048), Expect = 4.0e-110
Identity = 206/208 (99.04%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of Carg23720 vs. NCBI nr
Match: XP_023549992.1 (21 kDa protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 405.2 bits (1040), Expect = 3.4e-109
Identity = 205/208 (98.56%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPS IVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of Carg23720 vs. NCBI nr
Match: XP_022973542.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 401.7 bits (1031), Expect = 3.7e-108
Identity = 203/208 (97.60%), Postives = 205/208 (98.56%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPS IVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHV DYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDV+DGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of Carg23720 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.2e-29
Identity = 79/197 (40.10%), Postives = 115/197 (58.38%), Query Frame = 0


Query: 10  SLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQ 69
           +L+F+A L +L    +AA A       A  ++FI+TSC +T YP VC  SLS YA  IQ 
Sbjct: 7   TLVFSALLVIL----AAASA-------APANQFIKTSCTLTTYPAVCEQSLSAYAKTIQN 66

Query: 70  DPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGS 129
           +P  L   A+ +SL   ++   ++  L+     G   R  +A+HDC    +D++D +  S
Sbjct: 67  NPQELASTALQVSLTRTQQAQTFMKRLNKF--KGLKARQYAAIHDCLEEVEDSLDRVSRS 126

Query: 130 LKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKV 189
             +M+ ++      F F+MSNV+TW+SAALTD+ TC DGF    +DG IK+ V A+   V
Sbjct: 127 CDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAV 186

Query: 190 KKHTSNALALVNSFVEK 206
            + TSNALALVN+F  K
Sbjct: 187 ARVTSNALALVNNFAAK 190

[Close]

BLAST of Carg23720 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 7.5e-27
Identity = 76/193 (39.38%), Postives = 107/193 (55.44%), Query Frame = 0


Query: 17  LFVLRLVPSA-ADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLT 76
           LFVL L  +A   A      ++  +EFI+ SC  T YPD C+ SLS YA+ I++ P  L 
Sbjct: 11  LFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLA 70

Query: 77  RVAITISLANARRMAAYVSNLSHVGDY-GADHRVSSALHDCFTNFDDAVDEIRGSLKQMR 136
             A+ +S+A A+    YVS ++   DY G   R   A+ DC     D VD +  SLK+++
Sbjct: 71  ETALAVSIARAKSAKTYVSEMT---DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELK 130

Query: 137 QINDVDA-PSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKVKKHT 196
            + + D+   F F +SNV+TW SAALTD+  C DGF    + G +K  +      V + T
Sbjct: 131 HLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEET 190

Query: 197 SNALALVNSFVEK 206
           SNALAL+N F  K
Sbjct: 191 SNALALINDFASK 200

[Close]

BLAST of Carg23720 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.8e-26
Identity = 75/200 (37.50%), Postives = 117/200 (58.50%), Query Frame = 0


Query: 3   PQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSR 62
           P P P  S L +       L PS++ + T+ N T    ++I+TSC ITLY  +CY SLS 
Sbjct: 118 PPPPPSSSPLSS-------LSPSSS-SSTYSNQTN--LDYIKTSCNITLYKTICYNSLSP 177

Query: 63  YANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDA 122
           YA+ I+ +P  L  +A+ ++L++A+  + +V N+SH G  G       A+ DC     D+
Sbjct: 178 YASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGG--GLTRLEVVAVADCVEEIGDS 237

Query: 123 VDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVC 182
           V  ++ S++++  IN  D+  F   MS+V+TW+SAALT+ +TC DGF  +V   +K  V 
Sbjct: 238 VTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGF-SLVKTAVKDLVR 297

Query: 183 AKAEKVKKHTSNALALVNSF 203
               +V + TSNALAL+N +
Sbjct: 298 RHVVEVARLTSNALALINMY 304

[Close]

BLAST of Carg23720 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.2e-23
Identity = 65/166 (39.16%), Postives = 90/166 (54.22%), Query Frame = 0


Query: 42  FIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGD 101
           FI+ SC  T YP VC  SL+ YAN+IQ  P  L   A+ +++  A+    +V  L     
Sbjct: 38  FIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRF-- 97

Query: 102 YGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDA-PSFRFQMSNVQTWMSAALT 161
                R   A+ DC     DAVD +  S+ +++          F F MSN QTW SAALT
Sbjct: 98  TSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALT 157

Query: 162 DQETCTDGF-DDVVDGPIKKDVCAKAEKVKKHTSNALALVNSFVEK 206
           +  TC+DGF   V+DG +K  V A+   + + TSNALAL+N+F +K
Sbjct: 158 NANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAKK 201

[Close]

BLAST of Carg23720 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-22
Identity = 72/193 (37.31%), Postives = 109/193 (56.48%), Query Frame = 0


Query: 17  LFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQ-QDPASLT 76
           L +L  +     +   PN  ++  +FI +SC  T YP +C  +LS YA  I+  +   L 
Sbjct: 9   LVILLSITILQSSSATPN-RSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLA 68

Query: 77  RVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQM-R 136
           + A+ ISLA A+ +  +V+ L+         R   A+ DC     ++VD +  S+K++ R
Sbjct: 69  QTALIISLARAKSVTIFVAKLTKETP-KFKRREYLAIKDCIEVLGNSVDRLAQSVKELAR 128

Query: 137 QINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDD-VVDGPIKKDVCAKAEKVKKHTS 196
             + V +  F ++MSNVQTW+SAALTD+ TC DGF +  + G +K+ +  K   V + TS
Sbjct: 129 AGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTS 188

Query: 197 NALALVNSFVEKR 207
           NALALVN F EKR
Sbjct: 189 NALALVNQFAEKR 199

[Close]

BLAST of Carg23720 vs. ExPASy TrEMBL
Match: A0A6J1FD40 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111444672 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.9e-110
Identity = 206/208 (99.04%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of Carg23720 vs. ExPASy TrEMBL
Match: A0A6J1I7T1 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111472077 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.8e-108
Identity = 203/208 (97.60%), Postives = 205/208 (98.56%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQPS IVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHV DYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDV+DGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of Carg23720 vs. ExPASy TrEMBL
Match: A0A0A0KPD9 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G567800 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.8e-92
Identity = 178/208 (85.58%), Postives = 188/208 (90.38%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D    PNATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFYLRPVSADEDTPNSPNATANCMEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSHVGD GAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEK+IP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKKIP 208

[Close]

BLAST of Carg23720 vs. ExPASy TrEMBL
Match: A0A6J1JSR9 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111487516 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.9e-92
Identity = 179/212 (84.43%), Postives = 191/212 (90.09%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVP----SAADAGTFPNATADCSEFIRTSCFITLYPDVC 60
           MKPQ   I+SLLFAA L +LR VP    +AADAGT  NATA+ +EFIR+SC ITLYPDVC
Sbjct: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60

Query: 61  YTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCF 120
           Y SLSRYAN IQQDPASLTRVAITISLAN+RRMA+YVSNLSHVGDYGADHR +SALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGP 180
           TNFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 IKKDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
           +K DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 212

[Close]

BLAST of Carg23720 vs. ExPASy TrEMBL
Match: A0A5A7TTZ6 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00440 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.0e-92
Identity = 178/208 (85.58%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D     NATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFHLRPVFANEDTRNSSNATANCIEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSH GDYGAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHAGDYGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of Carg23720 vs. TAIR 10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 3.9e-47
Identity = 94/180 (52.22%), Postives = 134/180 (74.44%), Query Frame = 0


Query: 34  NATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYV 93
           ++ +D S+FIRTSC  TLYPD+C++SLS +++++  DPA L R AI+++L     +A+Y+
Sbjct: 25  HSQSDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARAAISVTLTKTLDLASYL 84

Query: 94  SNLSHV----GDYGADHRVSSAL-HDCFTNFDDAVDEIRGSLKQMRQ-INDVDAPSFRFQ 153
           +N++ +     + GA H  ++A+ HDCF N  DAV+E++GS+KQMR+ ++     SFRFQ
Sbjct: 85  ANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQMRELVSTGSLESFRFQ 144

Query: 154 MSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           MSNVQTW+SAALTD+ETCTDGF D+ D P K D+CA+ + VKK TSNALALVN  V+K I
Sbjct: 145 MSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204

[Close]

BLAST of Carg23720 vs. TAIR 10
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 2.8e-45
Identity = 100/191 (52.36%), Postives = 132/191 (69.11%), Query Frame = 0


Query: 17  LFVLRLVPSAADAGTFP--NATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASL 76
           LF+ R +     A  FP    T D  +FIRTSC  TLYPDVCYTSL+ YA+A+Q +PA L
Sbjct: 35  LFICRTI----SAVRFPPEQPTTDDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARL 94

Query: 77  TRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMR 136
            ++AI +SL+ A+  AAY+S LS           S+A+HDC +N  DAVD++RGSL+Q+R
Sbjct: 95  AKLAIGVSLSRAKYTAAYLSKLSR-------RAASAAVHDCVSNVGDAVDQMRGSLRQLR 154

Query: 137 QIND--VDAPSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKVKKH 196
           ++N      P+FRFQMSNVQTWMSAALTD+ETCTDG  +++ DG  K  +C +   VK+ 
Sbjct: 155 EMNHRRPGDPAFRFQMSNVQTWMSAALTDEETCTDGVTEEMEDGETKTAICDRVADVKRF 214

Query: 197 TSNALALVNSF 203
           TSNALALVN++
Sbjct: 215 TSNALALVNTY 214

[Close]

BLAST of Carg23720 vs. TAIR 10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 6.2e-45
Identity = 105/211 (49.76%), Postives = 140/211 (66.35%), Query Frame = 0


Query: 11  LLFAAGLFVLRLVPSAADAGTFPN-ATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQ 70
           LL    +  +RL+P        PN  T +  +FIRTSC  TLYPDVC+TSLS YA+A+Q 
Sbjct: 19  LLIFGSISAVRLLPR-------PNTTTTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQD 78

Query: 71  DPASLTRVAITISLANARRMAAYVSNLSH-VGDYGAD-HRVSSA-LHDCFTNFDDAVDEI 130
            PA L ++AI +SL+ A+  AA++S LS     Y  D H+ +SA + DC +N +DAVDE+
Sbjct: 79  SPARLAKLAIGVSLSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEM 138

Query: 131 RGSLKQMRQIND--------VDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGP-I 190
           RGSL+Q+R +N             +FRFQMSNVQTWMSAALTD++TCTDGF+D+ +G  I
Sbjct: 139 RGSLRQLRDMNGRGGGTAARRSVETFRFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLI 198

Query: 191 KKDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
           K  VC + E+VK+ TSNALALVN++     P
Sbjct: 199 KTTVCDRLEEVKRLTSNALALVNTYANNGAP 222

[Close]

BLAST of Carg23720 vs. TAIR 10
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 164.9 bits (416), Expect = 7.1e-41
Identity = 90/178 (50.56%), Postives = 121/178 (67.98%), Query Frame = 0


Query: 33  PNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAY 92
           P   ++ S+FIR +C  TLYPD+C+++LS +AN+IQ D   L RVAI+++L N   + +Y
Sbjct: 27  PLLPSNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNRLARVAISLTLHNTLHLLSY 86

Query: 93  VSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDA--PSFRFQMSN 152
           + N      Y  DH  +  L DCF N  DAVD +RGS+KQM+++        SFRFQMSN
Sbjct: 87  LQNA-----YNRDH-PTPVLRDCFENLKDAVDGMRGSMKQMKELVSASGSIESFRFQMSN 146

Query: 153 VQTWMSAALTDQETCTDGFDDV-VDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           V+TW+SAALTD+ TCTDGF DV  D  IK DVC++ + VKK TSNALALVN + ++ I
Sbjct: 147 VKTWLSAALTDEYTCTDGFKDVHEDDSIKDDVCSRVDDVKKLTSNALALVNRYADESI 198

[Close]

BLAST of Carg23720 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 122.1 bits (305), Expect = 5.3e-28
Identity = 76/193 (39.38%), Postives = 107/193 (55.44%), Query Frame = 0


Query: 17  LFVLRLVPSA-ADAGTFPNATADCSEFIRTSCFITLYPDVCYTSLSRYANAIQQDPASLT 76
           LFVL L  +A   A      ++  +EFI+ SC  T YPD C+ SLS YA+ I++ P  L 
Sbjct: 11  LFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLA 70

Query: 77  RVAITISLANARRMAAYVSNLSHVGDY-GADHRVSSALHDCFTNFDDAVDEIRGSLKQMR 136
             A+ +S+A A+    YVS ++   DY G   R   A+ DC     D VD +  SLK+++
Sbjct: 71  ETALAVSIARAKSAKTYVSEMT---DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELK 130

Query: 137 QINDVDA-PSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKVKKHT 196
            + + D+   F F +SNV+TW SAALTD+  C DGF    + G +K  +      V + T
Sbjct: 131 HLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEET 190

Query: 197 SNALALVNSFVEK 206
           SNALAL+N F  K
Sbjct: 191 SNALALINDFASK 200

The following BLAST results are available for this feature:

BLAST of Carg23720 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016778.1	2.8e-111	100.00	hypothetical protein SDJN02_21888, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6579262.1	4.0e-110	99.04	hypothetical protein SDJN03_23710, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022938421.1	4.0e-110	99.04	21 kDa protein-like [Cucurbita moschata]	[more]
XP_023549992.1	3.4e-109	98.56	21 kDa protein [Cucurbita pepo subsp. pepo]	[more]
XP_022973542.1	3.7e-108	97.60	21 kDa protein-like [Cucurbita maxima]	[more]

BLAST of Carg23720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	1.2e-29	40.10	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	7.5e-27	39.38	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	2.8e-26	37.50	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9STY5	2.2e-23	39.16	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI72	1.9e-22	37.31	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]

BLAST of Carg23720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FD40	1.9e-110	99.04	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111444672 PE=4 SV=1	[more]
A0A6J1I7T1	1.8e-108	97.60	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111472077 PE=4 SV=1	[more]
A0A0A0KPD9	1.8e-92	85.58	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G567800 PE=4 S...	[more]
A0A6J1JSR9	6.9e-92	84.43	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111487516 PE=4 SV=1	[more]
A0A5A7TTZ6	9.0e-92	85.58	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00440...	[more]

BLAST of Carg23720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23205.1	3.9e-47	52.22	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G14890.1	2.8e-45	52.36	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G01610.1	6.2e-45	49.76	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70720.1	7.1e-41	50.56	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	5.3e-28	39.38	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 38..198 e-value: 9.3E-44 score: 161.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 16..200 e-value: 1.5E-30 score: 103.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 42..198 e-value: 1.7E-39 score: 135.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 38..202 e-value: 1.1E-39 score: 137.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 40..202
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 17..205
None	No IPR available	PANTHER	PTHR31080:SF64	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 17..205
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 45..202 e-value: 7.41327E-48 score: 152.207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23720-RA	Carg23720-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity