Carg23536 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23536
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RNA-binding protein pno1
Location	Carg_Chr06: 3564930 .. 3566244 (-)
RNA-Seq Expression	Carg23536
Synteny	Carg23536

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGGATCGTTAAACCCTAAGAGAGCCGCCTCATTTCTTCGCACTTTCTCCTCTTCCTCTTGGCAGTTAACTTAGCACCTTCCCGCCGTCTCCTCCGTCGTTCTCTGTGCCACCGTCGACGCCGTACCCCTTCTTCACATATTTGTCTACCTTTCTTGGTCTCAGGTATTCTCCCTATTTACCCCATATTTGTCTACCTTTCAAGTTTCATTCCGTTGCAGTGGATGACATCCCAATGGGAACTGATTTTAAATTTTGTTTTGCCGAAGTTTCATTAGTTTGGTTGCTCATTCGTATGATCCGAGGCCGACGCATTCTATTCTTGTGTTACCTAAAATTGAAATTGGATTGTATATTTTAGAACCCGGCACTGTTTTTAGTTCAATCCTACTTGTTTGTTATTAGTTAATAGTTATATTGGGTTATAGTATCTTAGAATCAATGCATCTTTTACATGAGAAAAGGAATTTTTGTTTCTGAAATTCTTCTTGGGAGGATAAGAATGAAGTTTATCTGATTTATTTTCAATTTTTGTTGCTTATCATGTTGATGGGGTTTTGTTTTCATTTGATCTATTACTTCGTCGTTTAACACTAGTTCATTGGTTGTTTACAGTGTGGAAACTCAGAACAATTCTATAATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCATCTCAACCGAAGCCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAAATTTACACCCCAATTTATGAGCAGATGAAAATTGACATCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCGTTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACCTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAAAGGTTTTGA

mRNA sequence

AAAGGATCGTTAAACCCTAAGAGAGCCGCCTCATTTCTTCGCACTTTCTCCTCTTCCTCTTGGCAGTTAACTTAGCACCTTCCCGCCGTCTCCTCCGTCGTTCTCTGTGCCACCGTCGACGCCGTACCCCTTCTTCACATATTTGTCTACCTTTCTTGGTCTCAGTGTGGAAACTCAGAACAATTCTATAATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCATCTCAACCGAAGCCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAAATTTACACCCCAATTTATGAGCAGATGAAAATTGACATCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCGTTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACCTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAAAGGTTTTGA

Coding sequence (CDS)

ATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCATCTCAACCGAAGCCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAAATTTACACCCCAATTTATGAGCAGATGAAAATTGACATCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCGTTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACCTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAAAGGTTTTGA

Protein sequence

MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF

Homology

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BLAST of Carg23536 vs. NCBI nr
Match: XP_022951370.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita moschata] >XP_022951376.1 RNA-binding protein pno1-like isoform X1 [Cucurbita moschata] >KAG7028303.1 RNA-binding protein pno1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 434.5 bits (1116), Expect = 5.6e-118
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. NCBI nr
Match: XP_023005535.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita maxima] >XP_023005536.1 RNA-binding protein pno1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 433.0 bits (1112), Expect = 1.6e-117
Identity = 222/224 (99.11%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVEAVS+QPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. NCBI nr
Match: XP_023540523.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540524.1 RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432.2 bits (1110), Expect = 2.8e-117
Identity = 221/224 (98.66%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVEAVS+QPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIYEQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. NCBI nr
Match: XP_022951385.1 (RNA-binding protein pno1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 420.6 bits (1080), Expect = 8.3e-114
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 10  MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 69
           MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT
Sbjct: 1   MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 60

Query: 70  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE 129
           PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE
Sbjct: 61  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE 120

Query: 130 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 189
           LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN
Sbjct: 121 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 180

Query: 190 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 215

[Close]

BLAST of Carg23536 vs. NCBI nr
Match: XP_008449001.1 (PREDICTED: RNA-binding protein pno1 [Cucumis melo])

HSP 1 Score: 420.2 bits (1079), Expect = 1.1e-113
Identity = 215/224 (95.98%), Postives = 220/224 (98.21%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVE +S+Q K VVSTLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIY+QMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. ExPASy Swiss-Prot
Match: Q9VR89 (RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 3.9e-66
Identity = 123/187 (65.78%), Postives = 156/187 (83.42%), Query Frame = 0


Query: 37  PHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADT 96
           P +    R + RKVSVPPHRYS LK+ WM+I+TP+ E MK+ IR N+KAR+VEL+   +T
Sbjct: 53  PPKAKRARSELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPET 112

Query: 97  PDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLS 156
           PDI+NLQ+ ADFV AF+ GF+V DA+ALLR+++L+VESFEIKDVKTLRG+H SRAIGRL+
Sbjct: 113 PDIANLQRGADFVRAFLCGFEVDDALALLRLEDLFVESFEIKDVKTLRGDHQSRAIGRLA 172

Query: 157 GKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAV 216
           GK G+TKF IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LRAV
Sbjct: 173 GKGGRTKFTIENVTKTRIVLADSKIHILGSYQNIQLARRAVCNLILGSPPSKVYGNLRAV 232

Query: 217 TARLAER 224
            +RL+ER
Sbjct: 233 ASRLSER 239

[Close]

BLAST of Carg23536 vs. ExPASy Swiss-Prot
Match: Q29IG6 (RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l(1)G0004 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 4.4e-65
Identity = 120/178 (67.42%), Postives = 153/178 (85.96%), Query Frame = 0


Query: 46  QFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKC 105
           + RKVSVPPHRYS LK+ WM+I+TP+ E MK+ IR N+KAR+VEL+   +TPDI+NLQK 
Sbjct: 60  ELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPETPDIANLQKG 119

Query: 106 ADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFA 165
           ADFV AF+ GF+V DA+ALLR+++L+VE+FEIKDVKTLRG+H SRAIGRL+GK G+TKF 
Sbjct: 120 ADFVKAFLCGFEVDDALALLRLEDLFVETFEIKDVKTLRGDHQSRAIGRLAGKGGRTKFT 179

Query: 166 IENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAER 224
           IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LR+V +RL+ER
Sbjct: 180 IENVTKTRIVLADSKIHILGSYQNIQLARRAICNLILGSPPSKVYGNLRSVASRLSER 237

[Close]

BLAST of Carg23536 vs. ExPASy Swiss-Prot
Match: A7RP64 (RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.7e-65
Identity = 119/196 (60.71%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 29  KPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKV 88
           +P F P+K  ++  G+ + RK+ VP HRY+PLK+ WM+I+TP+ E +K+ IR NL +R V
Sbjct: 43  RPSFPPVKAQKLAGGKSETRKIPVPSHRYTPLKENWMKIFTPVVEHLKLQIRFNLGSRHV 102

Query: 89  ELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHL 148
           E++   +T DI  +QK ADFV AF+LGF+V DA+AL+R+D+L++ESFEI DVK L+G+HL
Sbjct: 103 EIRASKETSDIGAVQKAADFVQAFILGFEVEDALALIRLDDLFLESFEIADVKPLKGDHL 162

Query: 149 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 208
           SRAIGR++GK GKTKF IEN TKTRIV+A+TKIHILGSF NIKIAR ++C+LILGSP  K
Sbjct: 163 SRAIGRVAGKGGKTKFTIENVTKTRIVLAETKIHILGSFQNIKIARTAICNLILGSPPSK 222

Query: 209 VYSKLRAVTARLAERF 225
           VY  +RAV +R AERF
Sbjct: 223 VYGNMRAVASRSAERF 238

[Close]

BLAST of Carg23536 vs. ExPASy Swiss-Prot
Match: Q6VBQ6 (RNA-binding protein PNO1 OS=Oryzias latipes OX=8090 GN=pno1 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.3e-64
Identity = 119/196 (60.71%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 29  KPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKV 88
           +PHF P+   ++  G  + RKV+VP HRY+PLK+ W++I+TPI E +++ +R NLK R V
Sbjct: 49  RPHFPPISADKLR-GPDEMRKVAVPAHRYTPLKENWLKIFTPIVENLQLQVRFNLKTRNV 108

Query: 89  ELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHL 148
           E+KT  +T DI+ L K ADF+ AF+LGF V DA+AL+R+DEL++ESF++ DVK L+G+HL
Sbjct: 109 EIKTCKETQDIAALTKAADFIKAFVLGFQVDDAMALIRLDELFLESFDVTDVKPLKGDHL 168

Query: 149 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 208
           SRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+AR ++C+LILGSP  K
Sbjct: 169 SRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMARTAICNLILGSPPSK 228

Query: 209 VYSKLRAVTARLAERF 225
           VY  +RAV +R AERF
Sbjct: 229 VYGNIRAVASRTAERF 243

[Close]

BLAST of Carg23536 vs. ExPASy Swiss-Prot
Match: Q6VEU3 (RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2)

HSP 1 Score: 245.4 bits (625), Expect = 6.3e-64
Identity = 123/211 (58.29%), Postives = 161/211 (76.30%), Query Frame = 0


Query: 15  VSSQPKPVVSTLPPK-PHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYE 74
           + +Q +   S+ P K PHF  L   ++  G  + RKV VP HRY+PLK+ W++I+TPI E
Sbjct: 42  MDTQTEESSSSAPVKRPHFPALSGDQLGGGVDEMRKVPVPSHRYTPLKENWLKIFTPIVE 101

Query: 75  QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVE 134
            +++ +R NLK R+VE+KT  +T DI  L + ADFV AF+LGF V DA+AL+R+DEL++E
Sbjct: 102 NLQLQVRFNLKTRQVEIKTCKETQDIGALTRAADFVKAFVLGFQVEDALALIRLDELFLE 161

Query: 135 SFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIA 194
           +F++ DVK L+G+HLSRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+A
Sbjct: 162 TFDVTDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMA 221

Query: 195 RDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           R ++C+LILGSP  KVY  LRAV  R AERF
Sbjct: 222 RTAICNLILGSPPSKVYGNLRAVATRSAERF 252

[Close]

BLAST of Carg23536 vs. ExPASy TrEMBL
Match: A0A6J1GHF0 (RNA-binding protein pno1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454213 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 2.7e-118
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. ExPASy TrEMBL
Match: A0A6J1KXN9 (RNA-binding protein pno1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498497 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 7.9e-118
Identity = 222/224 (99.11%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVEAVS+QPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. ExPASy TrEMBL
Match: A0A6J1GIL7 (RNA-binding protein pno1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454213 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.0e-114
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 10  MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 69
           MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT
Sbjct: 1   MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 60

Query: 70  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE 129
           PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE
Sbjct: 61  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE 120

Query: 130 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 189
           LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN
Sbjct: 121 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 180

Query: 190 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 215

[Close]

BLAST of Carg23536 vs. ExPASy TrEMBL
Match: A0A1S3BLP2 (RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 5.3e-114
Identity = 215/224 (95.98%), Postives = 220/224 (98.21%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVE +S+Q K VVSTLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIY+QMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. ExPASy TrEMBL
Match: A0A6J1KTE6 (RNA-binding protein pno1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498497 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.2e-113
Identity = 213/215 (99.07%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 10  MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 69
           MEVEAVS+QPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT
Sbjct: 1   MEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 60

Query: 70  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE 129
           PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDA+ALLRVDE
Sbjct: 61  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDE 120

Query: 130 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 189
           LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN
Sbjct: 121 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 180

Query: 190 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 215

[Close]

BLAST of Carg23536 vs. TAIR 10
Match: AT3G13230.1 (RNA-binding KH domain-containing protein )

HSP 1 Score: 345.9 bits (886), Expect = 2.4e-95
Identity = 174/216 (80.56%), Postives = 197/216 (91.20%), Query Frame = 0


Query: 7   STSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWME 66
           ST MEVE  +    P    LPPKP F PLK HEM+DG+VQFRK++VPP+RYSPLKKAW++
Sbjct: 4   STQMEVETATEGTVP----LPPKPTFKPLKAHEMSDGKVQFRKIAVPPNRYSPLKKAWLD 63

Query: 67  IYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLR 126
           IYTPIY+QMK+DIRMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFD+ DA++LLR
Sbjct: 64  IYTPIYDQMKVDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDIPDAISLLR 123

Query: 127 VDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGS 186
           +DELYVESFEIKDVKTL+GEHLSRAIGRLSGK GKTKFAIEN+TKTRIVIADT+IHILG+
Sbjct: 124 MDELYVESFEIKDVKTLKGEHLSRAIGRLSGKGGKTKFAIENSTKTRIVIADTRIHILGA 183

Query: 187 FANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 223
           F+NIK+AR SLCSLI+GSPAGKVYSKLR+V+ARL E
Sbjct: 184 FSNIKVARSSLCSLIMGSPAGKVYSKLRSVSARLNE 215

The following BLAST results are available for this feature:

BLAST of Carg23536 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951370.1	5.6e-118	100.00	RNA-binding protein pno1-like isoform X1 [Cucurbita moschata] >XP_022951376.1 RN...	[more]
XP_023005535.1	1.6e-117	99.11	RNA-binding protein pno1-like isoform X1 [Cucurbita maxima] >XP_023005536.1 RNA-...	[more]
XP_023540523.1	2.8e-117	98.66	RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540...	[more]
XP_022951385.1	8.3e-114	100.00	RNA-binding protein pno1-like isoform X2 [Cucurbita moschata]	[more]
XP_008449001.1	1.1e-113	95.98	PREDICTED: RNA-binding protein pno1 [Cucumis melo]	[more]

BLAST of Carg23536 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9VR89	3.9e-66	65.78	RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV...	[more]
Q29IG6	4.4e-65	67.42	RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l...	[more]
A7RP64	5.7e-65	60.71	RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1	[more]
Q6VBQ6	1.3e-64	60.71	RNA-binding protein PNO1 OS=Oryzias latipes OX=8090 GN=pno1 PE=2 SV=1	[more]
Q6VEU3	6.3e-64	58.29	RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2	[more]

BLAST of Carg23536 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GHF0	2.7e-118	100.00	RNA-binding protein pno1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1KXN9	7.9e-118	99.11	RNA-binding protein pno1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1GIL7	4.0e-114	100.00	RNA-binding protein pno1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A1S3BLP2	5.3e-114	95.98	RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=3 SV=1	[more]
A0A6J1KTE6	1.2e-113	99.07	RNA-binding protein pno1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]

BLAST of Carg23536 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13230.1	2.4e-95	80.56	RNA-binding KH domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004087	K Homology domain	SMART	SM00322	kh_6	coord: 129..202 e-value: 1.7E-4 score: 31.0
IPR036612	K Homology domain, type 1 superfamily	GENE3D	3.30.1370.10	K Homology domain, type 1	coord: 128..224 e-value: 1.1E-22 score: 81.9
IPR036612	K Homology domain, type 1 superfamily	SUPERFAMILY	54791	Eukaryotic type KH-domain (KH-domain type I)	coord: 129..223
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..38
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR12826	RIBONUCLEASE Y	coord: 9..224
None	No IPR available	CDD	cd00105	KH-I	coord: 149..195 e-value: 0.00795288 score: 32.1468
IPR039912	Pre-rRNA-processing protein PNO1-like	PANTHER	PTHR12826:SF13	RNA-BINDING PROTEIN PNO1	coord: 9..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23536-RA	Carg23536-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding