Carg23334 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg23334 vs. NCBI nr
Match: KAG7029161.1 (Cathepsin B-like protease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 750.4 bits (1936), Expect = 7.2e-213
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA
Sbjct: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
           AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT
Sbjct: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK
Sbjct: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED
Sbjct: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI
Sbjct: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 349

BLAST of Carg23334 vs. NCBI nr
Match: XP_023540300.1 (cathepsin B-like protease 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 737.6 bits (1903), Expect = 4.8e-209
Identity = 342/349 (97.99%), Postives = 346/349 (99.14%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASSYFHSSLSLLFLV+FCFLHHHQVYA EQVL+FKLNADILQESIVRHVN+HPHAGWKA
Sbjct: 1   MASSYFHSSLSLLFLVSFCFLHHHQVYAGEQVLKFKLNADILQESIVRHVNKHPHAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
           AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSH KSLKLPKSFDAREAWPQCITIGT
Sbjct: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHPKSLKLPKSFDAREAWPQCITIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIW+KSKHYGVNAYRIK
Sbjct: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWRKSKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED
Sbjct: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI
Sbjct: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 349

BLAST of Carg23334 vs. NCBI nr
Match: XP_022932733.1 (cathepsin B-like protease 2 [Cucurbita moschata])

HSP 1 Score: 737.3 bits (1902), Expect = 6.3e-209
Identity = 342/349 (97.99%), Postives = 345/349 (98.85%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVL+FKLNADILQESIVRHVNEHPHAGWKA
Sbjct: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLKFKLNADILQESIVRHVNEHPHAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
           AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSH KSLKLPKSFDAREAWPQCITIGT
Sbjct: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHPKSLKLPKSFDAREAWPQCITIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIW+KSKHYGVNAYRIK
Sbjct: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWRKSKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHI GDELGGHAVKLIGWGTSDDGED
Sbjct: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHIIGDELGGHAVKLIGWGTSDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWN GWGDDGYFKI+RGTNECGIEEDVVAGLPSPRNIAREASI
Sbjct: 301 YWLLANQWNTGWGDDGYFKIRRGTNECGIEEDVVAGLPSPRNIAREASI 349

BLAST of Carg23334 vs. NCBI nr
Match: XP_022972180.1 (cathepsin B-like protease 2 [Cucurbita maxima])

HSP 1 Score: 736.1 bits (1899), Expect = 1.4e-208
Identity = 341/349 (97.71%), Postives = 346/349 (99.14%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASS+FHSSLSLLFLVAFCFLHHHQVYAEEQVL+FKLNADILQESIVRHVNEHPHAGWKA
Sbjct: 1   MASSHFHSSLSLLFLVAFCFLHHHQVYAEEQVLKFKLNADILQESIVRHVNEHPHAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
           AMNPSFSNYSVSQFKHILGVKQ+PEKDLKSTPVLSH KSLKLPKSFDAREAWPQCITIGT
Sbjct: 61  AMNPSFSNYSVSQFKHILGVKQSPEKDLKSTPVLSHPKSLKLPKSFDAREAWPQCITIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVT ECDPYFDTDGCSHPGCEPAY+TPKCVRHCVDKNQIW+KSKHYGVNAYRIK
Sbjct: 181 YFVRHGVVTAECDPYFDTDGCSHPGCEPAYNTPKCVRHCVDKNQIWRKSKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED
Sbjct: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI
Sbjct: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 349

BLAST of Carg23334 vs. NCBI nr
Match: XP_038903448.1 (cathepsin B-like protease 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 678.7 bits (1750), Expect = 2.6e-191
Identity = 312/349 (89.40%), Postives = 329/349 (94.27%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASS+ +SSLSLLFL A C  HH QVYAEEQVL+FK NADILQESIVRHVNEHP AGWKA
Sbjct: 1   MASSHLYSSLSLLFLAAVCTFHHQQVYAEEQVLEFKFNADILQESIVRHVNEHPQAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
            MNP FSNYSVSQFK++LGVKQTPEKDLKSTPVLSH KSLKLPKSFDAREAWPQCI+IGT
Sbjct: 61  TMNPRFSNYSVSQFKYLLGVKQTPEKDLKSTPVLSHPKSLKLPKSFDAREAWPQCISIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAF AVESLSDRFCIH++MNISLSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFGAVESLSDRFCIHFDMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVTE+CDPYFDT GCSHPGCEPAY TP+CVRHCVDKNQIW+K+KHYGVNAYRIK
Sbjct: 181 YFVRHGVVTEQCDPYFDTTGCSHPGCEPAYPTPRCVRHCVDKNQIWRKTKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
            DP DIMAEVYKNGPVEV FTVYEDFAHYKSGVYK+ITGD +GGHAVKLIGWGT+DDGED
Sbjct: 241 ADPSDIMAEVYKNGPVEVSFTVYEDFAHYKSGVYKYITGDVMGGHAVKLIGWGTTDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWNRGWGDDGYFKI+RGTNECGIEEDVVAGLPS RNIAREA+I
Sbjct: 301 YWLLANQWNRGWGDDGYFKIRRGTNECGIEEDVVAGLPSARNIAREAAI 349

BLAST of Carg23334 vs. ExPASy Swiss-Prot
Match: Q93VC9 (Cathepsin B-like protease 2 OS=Arabidopsis thaliana OX=3702 GN=CATHB2 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-149
Identity = 236/321 (73.52%), Postives = 268/321 (83.49%), Query Frame = 0

Query: 25  QVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKAAMNPSFSNYSVSQFKHILGVKQTP 84
           Q  A E + + KL + ILQ  IV+ VNE+P+AGWKA+ N  F+N +V++FK +LGVK TP
Sbjct: 29  QGIAAENLSKQKLTSWILQNEIVKEVNENPNAGWKASFNDRFANATVAEFKRLLGVKPTP 88

Query: 85  EKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGTILGQGHCGSCWAFAAVESLSDRFC 144
           + +    P++SH  SLKLPK FDAR AW QC +IG IL QGHCGSCWAF AVESLSDRFC
Sbjct: 89  KTEFLGVPIVSHDISLKLPKEFDARTAWSQCTSIGRILDQGHCGSCWAFGAVESLSDRFC 148

Query: 145 IHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWRYFVRHGVVTEECDPYFDTDGCSHP 204
           I YNMN+SLSVNDLLACCGF+CG GC+GGYPI+AWRYF  HGVVTEECDPYFD  GCSHP
Sbjct: 149 IKYNMNVSLSVNDLLACCGFLCGQGCNGGYPIAAWRYFKHHGVVTEECDPYFDNTGCSHP 208

Query: 205 GCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFTVYE 264
           GCEPAY TPKC R CV  NQ+W++SKHYGV+AY+++  P DIMAEVYKNGPVEV FTVYE
Sbjct: 209 GCEPAYPTPKCARKCVSGNQLWRESKHYGVSAYKVRSHPDDIMAEVYKNGPVEVAFTVYE 268

Query: 265 DFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGEDYWLLANQWNRGWGDDGYFKIKRGT 324
           DFAHYKSGVYKHITG  +GGHAVKLIGWGTSDDGEDYWLLANQWNR WGDDGYFKI+RGT
Sbjct: 269 DFAHYKSGVYKHITGTNIGGHAVKLIGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGT 328

Query: 325 NECGIEEDVVAGLPSPRNIAR 346
           NECGIE  VVAGLPS RN+ +
Sbjct: 329 NECGIEHGVVAGLPSDRNVVK 349

BLAST of Carg23334 vs. ExPASy Swiss-Prot
Match: Q94K85 (Cathepsin B-like protease 3 OS=Arabidopsis thaliana OX=3702 GN=CATHB3 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.1e-147
Identity = 231/316 (73.10%), Postives = 266/316 (84.18%), Query Frame = 0

Query: 30  EQVLQFKLNADILQESIVRHVNEHPHAGWKAAMNPSFSNYSVSQFKHILGVKQTPEKDLK 89
           E + + KL++ ILQ+ IV+ VNE+P+AGWKAA+N  FSN +V++FK +LGVK TP+K   
Sbjct: 31  ESLTKQKLDSKILQDEIVKKVNENPNAGWKAAINDRFSNATVAEFKRLLGVKPTPKKHFL 90

Query: 90  STPVLSHSKSLKLPKSFDAREAWPQCITIGTILGQGHCGSCWAFAAVESLSDRFCIHYNM 149
             P++SH  SLKLPK+FDAR AWPQC +IG IL QGHCGSCWAF AVESLSDRFCI + M
Sbjct: 91  GVPIVSHDPSLKLPKAFDARTAWPQCTSIGNILDQGHCGSCWAFGAVESLSDRFCIQFGM 150

Query: 150 NISLSVNDLLACCGFMCGDGCDGGYPISAWRYFVRHGVVTEECDPYFDTDGCSHPGCEPA 209
           NISLSVNDLLACCGF CGDGCDGGYPI+AW+YF   GVVTEECDPYFD  GCSHPGCEPA
Sbjct: 151 NISLSVNDLLACCGFRCGDGCDGGYPIAAWQYFSYSGVVTEECDPYFDNTGCSHPGCEPA 210

Query: 210 YSTPKCVRHCVDKNQIWKKSKHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFTVYEDFAHY 269
           Y TPKC R CV  N++W +SKHY V+ Y +K +P DIMAEVYKNGPVEV FTVYEDFAHY
Sbjct: 211 YPTPKCSRKCVSDNKLWSESKHYSVSTYTVKSNPQDIMAEVYKNGPVEVSFTVYEDFAHY 270

Query: 270 KSGVYKHITGDELGGHAVKLIGWGTSDDGEDYWLLANQWNRGWGDDGYFKIKRGTNECGI 329
           KSGVYKHITG  +GGHAVKLIGWGTS +GEDYWL+ANQWNRGWGDDGYF I+RGTNECGI
Sbjct: 271 KSGVYKHITGSNIGGHAVKLIGWGTSSEGEDYWLMANQWNRGWGDDGYFMIRRGTNECGI 330

Query: 330 EEDVVAGLPSPRNIAR 346
           E++ VAGLPS +N+ R
Sbjct: 331 EDEPVAGLPSSKNVFR 346

BLAST of Carg23334 vs. ExPASy Swiss-Prot
Match: F4HVZ1 (Cathepsin B-like protease 1 OS=Arabidopsis thaliana OX=3702 GN=CATHB1 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 3.0e-137
Identity = 229/354 (64.69%), Postives = 266/354 (75.14%), Query Frame = 0

Query: 10  LSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKAAMNPSFSNY 69
           L+ +FL+ F   +   + AE    Q KL + ILQ  IV+ VNE+P+AGWKAA N  F+N 
Sbjct: 12  LASVFLLLFSSFNLQGIAAENLSKQ-KLTSLILQNEIVKEVNENPNAGWKAAFNDRFANA 71

Query: 70  SVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQC-----ITIGTILGQ 129
           +V++FK +LGV QTP+      P++ H  SLKLPK FDAR AW  C     I +G IL  
Sbjct: 72  TVAEFKRLLGVIQTPKTAYLGVPIVRHDLSLKLPKEFDARTAWSHCTSIRRILVGYILNN 131

Query: 130 ---------------GHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDG 189
                          GHCGSCWAF AVESLSDRFCI YN+N+SLS ND++ACCG +CG G
Sbjct: 132 VLLWSTITLWFWFLLGHCGSCWAFGAVESLSDRFCIKYNLNVSLSANDVIACCGLLCGFG 191

Query: 190 CDGGYPISAWRYFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKS 249
           C+GG+P+ AW YF  HGVVT+ECDPYFD  GCSHPGCEP Y TPKC R CV +NQ+W +S
Sbjct: 192 CNGGFPMGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGES 251

Query: 250 KHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKL 309
           KHYGV AYRI  DP DIMAEVYKNGPVEV FTVYEDFAHYKSGVYK+ITG ++GGHAVKL
Sbjct: 252 KHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKL 311

Query: 310 IGWGTSDDGEDYWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNI 344
           IGWGTSDDGEDYWLLANQWNR WGDDGYFKI+RGTNECGIE+ VVAGLPS +N+
Sbjct: 312 IGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGTNECGIEQSVVAGLPSEKNV 364

BLAST of Carg23334 vs. ExPASy Swiss-Prot
Match: Q4R5M2 (Cathepsin B OS=Macaca fascicularis OX=9541 GN=CTSB PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 3.0e-81
Identity = 152/317 (47.95%), Postives = 201/317 (63.41%), Query Frame = 0

Query: 42  LQESIVRHVNEHPHAGWKAAMNPSFSNYSVSQFKHI----LGVKQTPEKDLKSTPVLSHS 101
           L + +V +VN+  +  W+A  N  F N  VS  K +    LG  + P++       +  +
Sbjct: 26  LSDELVNYVNKQ-NTTWQAGHN--FYNVDVSYLKRLCGTFLGGPKPPQR-------VMFT 85

Query: 102 KSLKLPKSFDAREAWPQCITIGTILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVN- 161
           + LKLP+SFDARE WPQC TI  I  QG CGSCWAF AVE++SDR CIH N ++S+ V+ 
Sbjct: 86  EDLKLPESFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSA 145

Query: 162 -DLLACCGFMCGDGCDGGYPISAWRYFVRHGVVT-------EECDPYFDTDGCSH----- 221
            DLL CCG MCGDGC+GGYP  AW ++ R G+V+         C PY     C H     
Sbjct: 146 EDLLTCCGIMCGDGCNGGYPAGAWNFWTRKGLVSGGLYDSHVGCRPY-SIPPCEHHVNGS 205

Query: 222 -PGCEPAYSTPKCVRHC-VDKNQIWKKSKHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFT 281
            P C     TPKC + C    +  +K+ KHYG N+Y +     DIMAE+YKNGPVE  F+
Sbjct: 206 RPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFS 265

Query: 282 VYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGEDYWLLANQWNRGWGDDGYFKIK 339
           VY DF  YKSGVY+H+TG+ +GGHA++++GWG  ++G  YWL+AN WN  WGD+G+FKI 
Sbjct: 266 VYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGV-ENGTPYWLVANSWNTDWGDNGFFKIL 325

BLAST of Carg23334 vs. ExPASy Swiss-Prot
Match: Q5R6D1 (Cathepsin B OS=Pongo abelii OX=9601 GN=CTSB PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 6.8e-81
Identity = 152/317 (47.95%), Postives = 201/317 (63.41%), Query Frame = 0

Query: 42  LQESIVRHVNEHPHAGWKAAMNPSFSNYSVSQFKHI----LGVKQTPEKDLKSTPVLSHS 101
           L + +V +VN+  +  W+A  N  F N  VS  K +    LG  + P++       +  +
Sbjct: 26  LSDELVNYVNKR-NTTWQAGHN--FYNVDVSYLKKLCGTFLGGPKPPQR-------VMFT 85

Query: 102 KSLKLPKSFDAREAWPQCITIGTILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVN- 161
           + LKLP+SFDARE WPQC TI  I  QG CGSCWAF AVE++SDR CIH N ++S+ V+ 
Sbjct: 86  EDLKLPESFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSA 145

Query: 162 -DLLACCGFMCGDGCDGGYPISAWRYFVRHGVVT-------EECDPYFDTDGCSH----- 221
            DLL CCG MCGDGC+GGYP  AW ++ R G+V+         C PY     C H     
Sbjct: 146 EDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPY-SIPPCEHHVNGS 205

Query: 222 -PGCEPAYSTPKCVRHC-VDKNQIWKKSKHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFT 281
            P C     TPKC + C    +  +K+ KHYG N+Y +     DIMAE+YKNGPVE  F+
Sbjct: 206 RPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSERDIMAEIYKNGPVEGAFS 265

Query: 282 VYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGEDYWLLANQWNRGWGDDGYFKIK 339
           VY DF  YKSGVY+H+TG+ +GGHA++++GWG  ++G  YWL+AN WN  WGD+G+FKI 
Sbjct: 266 VYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGV-ENGTPYWLVANSWNTDWGDNGFFKIL 325

BLAST of Carg23334 vs. ExPASy TrEMBL
Match: A0A6J1F2K3 (cathepsin B-like protease 2 OS=Cucurbita moschata OX=3662 GN=LOC111439194 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 3.0e-209
Identity = 342/349 (97.99%), Postives = 345/349 (98.85%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVL+FKLNADILQESIVRHVNEHPHAGWKA
Sbjct: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLKFKLNADILQESIVRHVNEHPHAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
           AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSH KSLKLPKSFDAREAWPQCITIGT
Sbjct: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHPKSLKLPKSFDAREAWPQCITIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIW+KSKHYGVNAYRIK
Sbjct: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWRKSKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHI GDELGGHAVKLIGWGTSDDGED
Sbjct: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHIIGDELGGHAVKLIGWGTSDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWN GWGDDGYFKI+RGTNECGIEEDVVAGLPSPRNIAREASI
Sbjct: 301 YWLLANQWNTGWGDDGYFKIRRGTNECGIEEDVVAGLPSPRNIAREASI 349

BLAST of Carg23334 vs. ExPASy TrEMBL
Match: A0A6J1I7T5 (cathepsin B-like protease 2 OS=Cucurbita maxima OX=3661 GN=LOC111470792 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 6.8e-209
Identity = 341/349 (97.71%), Postives = 346/349 (99.14%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASS+FHSSLSLLFLVAFCFLHHHQVYAEEQVL+FKLNADILQESIVRHVNEHPHAGWKA
Sbjct: 1   MASSHFHSSLSLLFLVAFCFLHHHQVYAEEQVLKFKLNADILQESIVRHVNEHPHAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
           AMNPSFSNYSVSQFKHILGVKQ+PEKDLKSTPVLSH KSLKLPKSFDAREAWPQCITIGT
Sbjct: 61  AMNPSFSNYSVSQFKHILGVKQSPEKDLKSTPVLSHPKSLKLPKSFDAREAWPQCITIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVT ECDPYFDTDGCSHPGCEPAY+TPKCVRHCVDKNQIW+KSKHYGVNAYRIK
Sbjct: 181 YFVRHGVVTAECDPYFDTDGCSHPGCEPAYNTPKCVRHCVDKNQIWRKSKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED
Sbjct: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI
Sbjct: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 349

BLAST of Carg23334 vs. ExPASy TrEMBL
Match: A0A0A0LFN4 (Pept_C1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G844870 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 4.9e-191
Identity = 309/349 (88.54%), Postives = 331/349 (94.84%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASS+F+ SLSLLFL A C  HH QVYAEEQVL+FKL+ADILQESIVRHVNEHP AGWKA
Sbjct: 1   MASSHFYLSLSLLFLAAVCTFHHQQVYAEEQVLKFKLDADILQESIVRHVNEHPQAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
            MNP FSNYSVSQFK++LGVKQTPEKDLKSTPVLSH KSLKLPKSFDAREAWPQCI+IGT
Sbjct: 61  TMNPRFSNYSVSQFKYLLGVKQTPEKDLKSTPVLSHPKSLKLPKSFDAREAWPQCISIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAF AVESLSDRFCIH++MNI+LSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFGAVESLSDRFCIHFDMNITLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVTE+CDPYFDT GCSHPGCEPAY TP+CVRHCVDKNQIW+K+KHYGV+AYR+K
Sbjct: 181 YFVRHGVVTEQCDPYFDTTGCSHPGCEPAYPTPRCVRHCVDKNQIWRKTKHYGVSAYRVK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           +DP DIMAEVYKNGPVEV FTVYEDFAHYKSGVYKHITGD +GGHAVKLIGWGT+DDGED
Sbjct: 241 RDPNDIMAEVYKNGPVEVSFTVYEDFAHYKSGVYKHITGDVMGGHAVKLIGWGTTDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWNRGWGDDGYFKI+RGTNECGIEEDVVAGLPS +NIAREA+I
Sbjct: 301 YWLLANQWNRGWGDDGYFKIRRGTNECGIEEDVVAGLPSTKNIAREAAI 349

BLAST of Carg23334 vs. ExPASy TrEMBL
Match: A0A1S3CNJ5 (cathepsin B-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502979 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 2.7e-189
Identity = 308/349 (88.25%), Postives = 329/349 (94.27%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASS  + SLSLLFL A C  HH QV+AEEQVL+FKL+ADILQESIVRHVNEHP AGWKA
Sbjct: 1   MASSQLYLSLSLLFLAAVCTFHHQQVHAEEQVLKFKLDADILQESIVRHVNEHPQAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
            MNP FSNYSVSQFK++LGVKQTPEKDLKSTPVLSH KSL+LPKSFDAREAWPQCI+IGT
Sbjct: 61  TMNPRFSNYSVSQFKYLLGVKQTPEKDLKSTPVLSHPKSLRLPKSFDAREAWPQCISIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAF AVESLSDRFCIH++MNI+LSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFGAVESLSDRFCIHFDMNITLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVTE+CDPYFDT GCSHPGCEPAY TP+CVRHCVDKNQIW+K+KHYGVNAYRIK
Sbjct: 181 YFVRHGVVTEQCDPYFDTTGCSHPGCEPAYPTPRCVRHCVDKNQIWRKTKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           KDP DIMAEVYKNGPVEV FTVYEDFAHYKSGVYK+ITGD +GGHAVKLIGWGT+DDGED
Sbjct: 241 KDPNDIMAEVYKNGPVEVSFTVYEDFAHYKSGVYKYITGDVMGGHAVKLIGWGTTDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWNRGWG+DGYFKI+RGTNECGIEEDVVAGLPS RNIAREA+I
Sbjct: 301 YWLLANQWNRGWGEDGYFKIRRGTNECGIEEDVVAGLPSTRNIAREAAI 349

BLAST of Carg23334 vs. ExPASy TrEMBL
Match: A0A5A7U7U4 (Cathepsin B-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00200 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 5.0e-188
Identity = 308/349 (88.25%), Postives = 329/349 (94.27%), Query Frame = 0

Query: 1   MASSYFHSSLSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKA 60
           MASS  + SLSLLFL A C   HHQV+AEEQVL+FKL+ADILQESIVRHVNEHP AGWKA
Sbjct: 1   MASSQLYLSLSLLFLAAVC-TFHHQVHAEEQVLKFKLDADILQESIVRHVNEHPQAGWKA 60

Query: 61  AMNPSFSNYSVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGT 120
            MNP FSNYSVSQFK++LGVKQTPEKDLKSTPVLSH KSL+LPKSFDAREAWPQCI+IGT
Sbjct: 61  TMNPRFSNYSVSQFKYLLGVKQTPEKDLKSTPVLSHPKSLRLPKSFDAREAWPQCISIGT 120

Query: 121 ILGQGHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWR 180
           IL QGHCGSCWAF AVESLSDRFCIH++MNI+LSVNDLLACCGFMCGDGCDGGYPISAWR
Sbjct: 121 ILDQGHCGSCWAFGAVESLSDRFCIHFDMNITLSVNDLLACCGFMCGDGCDGGYPISAWR 180

Query: 181 YFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIK 240
           YFVRHGVVTE+CDPYFDT GCSHPGCEPAY TP+CVRHCVDKNQIW+K+KHYGVNAYRIK
Sbjct: 181 YFVRHGVVTEQCDPYFDTTGCSHPGCEPAYPTPRCVRHCVDKNQIWRKTKHYGVNAYRIK 240

Query: 241 KDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGED 300
           KDP DIMAEVYKNGPVEV FTVYEDFAHYKSGVYK+ITGD +GGHAVKLIGWGT+DDGED
Sbjct: 241 KDPNDIMAEVYKNGPVEVSFTVYEDFAHYKSGVYKYITGDVMGGHAVKLIGWGTTDDGED 300

Query: 301 YWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNIAREASI 350
           YWLLANQWNRGWG+DGYFKI+RGTNECGIEEDVVAGLPS RNIAREA+I
Sbjct: 301 YWLLANQWNRGWGEDGYFKIRRGTNECGIEEDVVAGLPSTRNIAREAAI 348

BLAST of Carg23334 vs. TAIR 10
Match: AT1G02305.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 530.0 bits (1364), Expect = 1.4e-150
Identity = 236/321 (73.52%), Postives = 268/321 (83.49%), Query Frame = 0

Query: 25  QVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKAAMNPSFSNYSVSQFKHILGVKQTP 84
           Q  A E + + KL + ILQ  IV+ VNE+P+AGWKA+ N  F+N +V++FK +LGVK TP
Sbjct: 29  QGIAAENLSKQKLTSWILQNEIVKEVNENPNAGWKASFNDRFANATVAEFKRLLGVKPTP 88

Query: 85  EKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGTILGQGHCGSCWAFAAVESLSDRFC 144
           + +    P++SH  SLKLPK FDAR AW QC +IG IL QGHCGSCWAF AVESLSDRFC
Sbjct: 89  KTEFLGVPIVSHDISLKLPKEFDARTAWSQCTSIGRILDQGHCGSCWAFGAVESLSDRFC 148

Query: 145 IHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWRYFVRHGVVTEECDPYFDTDGCSHP 204
           I YNMN+SLSVNDLLACCGF+CG GC+GGYPI+AWRYF  HGVVTEECDPYFD  GCSHP
Sbjct: 149 IKYNMNVSLSVNDLLACCGFLCGQGCNGGYPIAAWRYFKHHGVVTEECDPYFDNTGCSHP 208

Query: 205 GCEPAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFTVYE 264
           GCEPAY TPKC R CV  NQ+W++SKHYGV+AY+++  P DIMAEVYKNGPVEV FTVYE
Sbjct: 209 GCEPAYPTPKCARKCVSGNQLWRESKHYGVSAYKVRSHPDDIMAEVYKNGPVEVAFTVYE 268

Query: 265 DFAHYKSGVYKHITGDELGGHAVKLIGWGTSDDGEDYWLLANQWNRGWGDDGYFKIKRGT 324
           DFAHYKSGVYKHITG  +GGHAVKLIGWGTSDDGEDYWLLANQWNR WGDDGYFKI+RGT
Sbjct: 269 DFAHYKSGVYKHITGTNIGGHAVKLIGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGT 328

Query: 325 NECGIEEDVVAGLPSPRNIAR 346
           NECGIE  VVAGLPS RN+ +
Sbjct: 329 NECGIEHGVVAGLPSDRNVVK 349

BLAST of Carg23334 vs. TAIR 10
Match: AT4G01610.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 524.2 bits (1349), Expect = 7.8e-149
Identity = 231/316 (73.10%), Postives = 266/316 (84.18%), Query Frame = 0

Query: 30  EQVLQFKLNADILQESIVRHVNEHPHAGWKAAMNPSFSNYSVSQFKHILGVKQTPEKDLK 89
           E + + KL++ ILQ+ IV+ VNE+P+AGWKAA+N  FSN +V++FK +LGVK TP+K   
Sbjct: 31  ESLTKQKLDSKILQDEIVKKVNENPNAGWKAAINDRFSNATVAEFKRLLGVKPTPKKHFL 90

Query: 90  STPVLSHSKSLKLPKSFDAREAWPQCITIGTILGQGHCGSCWAFAAVESLSDRFCIHYNM 149
             P++SH  SLKLPK+FDAR AWPQC +IG IL QGHCGSCWAF AVESLSDRFCI + M
Sbjct: 91  GVPIVSHDPSLKLPKAFDARTAWPQCTSIGNILDQGHCGSCWAFGAVESLSDRFCIQFGM 150

Query: 150 NISLSVNDLLACCGFMCGDGCDGGYPISAWRYFVRHGVVTEECDPYFDTDGCSHPGCEPA 209
           NISLSVNDLLACCGF CGDGCDGGYPI+AW+YF   GVVTEECDPYFD  GCSHPGCEPA
Sbjct: 151 NISLSVNDLLACCGFRCGDGCDGGYPIAAWQYFSYSGVVTEECDPYFDNTGCSHPGCEPA 210

Query: 210 YSTPKCVRHCVDKNQIWKKSKHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFTVYEDFAHY 269
           Y TPKC R CV  N++W +SKHY V+ Y +K +P DIMAEVYKNGPVEV FTVYEDFAHY
Sbjct: 211 YPTPKCSRKCVSDNKLWSESKHYSVSTYTVKSNPQDIMAEVYKNGPVEVSFTVYEDFAHY 270

Query: 270 KSGVYKHITGDELGGHAVKLIGWGTSDDGEDYWLLANQWNRGWGDDGYFKIKRGTNECGI 329
           KSGVYKHITG  +GGHAVKLIGWGTS +GEDYWL+ANQWNRGWGDDGYF I+RGTNECGI
Sbjct: 271 KSGVYKHITGSNIGGHAVKLIGWGTSSEGEDYWLMANQWNRGWGDDGYFMIRRGTNECGI 330

Query: 330 EEDVVAGLPSPRNIAR 346
           E++ VAGLPS +N+ R
Sbjct: 331 EDEPVAGLPSSKNVFR 346

BLAST of Carg23334 vs. TAIR 10
Match: AT4G01610.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 524.2 bits (1349), Expect = 7.8e-149
Identity = 231/316 (73.10%), Postives = 266/316 (84.18%), Query Frame = 0

Query: 30  EQVLQFKLNADILQESIVRHVNEHPHAGWKAAMNPSFSNYSVSQFKHILGVKQTPEKDLK 89
           E + + KL++ ILQ+ IV+ VNE+P+AGWKAA+N  FSN +V++FK +LGVK TP+K   
Sbjct: 31  ESLTKQKLDSKILQDEIVKKVNENPNAGWKAAINDRFSNATVAEFKRLLGVKPTPKKHFL 90

Query: 90  STPVLSHSKSLKLPKSFDAREAWPQCITIGTILGQGHCGSCWAFAAVESLSDRFCIHYNM 149
             P++SH  SLKLPK+FDAR AWPQC +IG ILG GHCGSCWAF AVESLSDRFCI + M
Sbjct: 91  GVPIVSHDPSLKLPKAFDARTAWPQCTSIGNILGLGHCGSCWAFGAVESLSDRFCIQFGM 150

Query: 150 NISLSVNDLLACCGFMCGDGCDGGYPISAWRYFVRHGVVTEECDPYFDTDGCSHPGCEPA 209
           NISLSVNDLLACCGF CGDGCDGGYPI+AW+YF   GVVTEECDPYFD  GCSHPGCEPA
Sbjct: 151 NISLSVNDLLACCGFRCGDGCDGGYPIAAWQYFSYSGVVTEECDPYFDNTGCSHPGCEPA 210

Query: 210 YSTPKCVRHCVDKNQIWKKSKHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFTVYEDFAHY 269
           Y TPKC R CV  N++W +SKHY V+ Y +K +P DIMAEVYKNGPVEV FTVYEDFAHY
Sbjct: 211 YPTPKCSRKCVSDNKLWSESKHYSVSTYTVKSNPQDIMAEVYKNGPVEVSFTVYEDFAHY 270

Query: 270 KSGVYKHITGDELGGHAVKLIGWGTSDDGEDYWLLANQWNRGWGDDGYFKIKRGTNECGI 329
           KSGVYKHITG  +GGHAVKLIGWGTS +GEDYWL+ANQWNRGWGDDGYF I+RGTNECGI
Sbjct: 271 KSGVYKHITGSNIGGHAVKLIGWGTSSEGEDYWLMANQWNRGWGDDGYFMIRRGTNECGI 330

Query: 330 EEDVVAGLPSPRNIAR 346
           E++ VAGLPS +N+ R
Sbjct: 331 EDEPVAGLPSSKNVFR 346

BLAST of Carg23334 vs. TAIR 10
Match: AT1G02300.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.1e-138
Identity = 229/354 (64.69%), Postives = 266/354 (75.14%), Query Frame = 0

Query: 10  LSLLFLVAFCFLHHHQVYAEEQVLQFKLNADILQESIVRHVNEHPHAGWKAAMNPSFSNY 69
           L+ +FL+ F   +   + AE    Q KL + ILQ  IV+ VNE+P+AGWKAA N  F+N 
Sbjct: 12  LASVFLLLFSSFNLQGIAAENLSKQ-KLTSLILQNEIVKEVNENPNAGWKAAFNDRFANA 71

Query: 70  SVSQFKHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQC-----ITIGTILGQ 129
           +V++FK +LGV QTP+      P++ H  SLKLPK FDAR AW  C     I +G IL  
Sbjct: 72  TVAEFKRLLGVIQTPKTAYLGVPIVRHDLSLKLPKEFDARTAWSHCTSIRRILVGYILNN 131

Query: 130 ---------------GHCGSCWAFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDG 189
                          GHCGSCWAF AVESLSDRFCI YN+N+SLS ND++ACCG +CG G
Sbjct: 132 VLLWSTITLWFWFLLGHCGSCWAFGAVESLSDRFCIKYNLNVSLSANDVIACCGLLCGFG 191

Query: 190 CDGGYPISAWRYFVRHGVVTEECDPYFDTDGCSHPGCEPAYSTPKCVRHCVDKNQIWKKS 249
           C+GG+P+ AW YF  HGVVT+ECDPYFD  GCSHPGCEP Y TPKC R CV +NQ+W +S
Sbjct: 192 CNGGFPMGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGES 251

Query: 250 KHYGVNAYRIKKDPYDIMAEVYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELGGHAVKL 309
           KHYGV AYRI  DP DIMAEVYKNGPVEV FTVYEDFAHYKSGVYK+ITG ++GGHAVKL
Sbjct: 252 KHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKL 311

Query: 310 IGWGTSDDGEDYWLLANQWNRGWGDDGYFKIKRGTNECGIEEDVVAGLPSPRNI 344
           IGWGTSDDGEDYWLLANQWNR WGDDGYFKI+RGTNECGIE+ VVAGLPS +N+
Sbjct: 312 IGWGTSDDGEDYWLLANQWNRSWGDDGYFKIRRGTNECGIEQSVVAGLPSEKNV 364

BLAST of Carg23334 vs. TAIR 10
Match: AT3G45310.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-27
Identity = 94/323 (29.10%), Postives = 144/323 (44.58%), Query Frame = 0

Query: 15  LVAFCFLHHHQVYAEEQVLQFKLNADILQES--IVRHVNEHPHAGWKAAMNPSFSNYSVS 74
           +++F    H      + V + KL   + +E+  ++R  N+     +K ++N  F++ +  
Sbjct: 56  VLSFSRFTHRYGKKYQSVEEMKLRFSVFKENLDLIRSTNK-KGLSYKLSLN-QFADLTWQ 115

Query: 75  QF-KHILGVKQTPEKDLKSTPVLSHSKSLKLPKSFDAREAWPQCITIGTILGQGHCGSCW 134
           +F ++ LG  Q     LK +  ++ +    +P + D    W +   +  +  QGHCGSCW
Sbjct: 116 EFQRYKLGAAQNCSATLKGSHKITEA---TVPDTKD----WREDGIVSPVKEQGHCGSCW 175

Query: 135 AFAAVESLSDRFCIHYNMNISLSVNDLLACCGFMCGDGCDGGYPISAWRYF-VRHGVVTE 194
            F+   +L   +   +   ISLS   L+ C G     GC GG P  A+ Y     G+ TE
Sbjct: 176 TFSTTGALEAAYHQAFGKGISLSEQQLVDCAGTFNNFGCHGGLPSQAFEYIKYNGGLDTE 235

Query: 195 ECDPYFDTDGCSHPGCE-PAYSTPKCVRHCVDKNQIWKKSKHYGVNAYRIKKDPYDIMAE 254
           E  PY   DG    GC+  A +    VR  V+     +    + V   R           
Sbjct: 236 EAYPYTGKDG----GCKFSAKNIGVQVRDSVNITLGAEDELKHAVGLVR----------- 295

Query: 255 VYKNGPVEVDFTVYEDFAHYKSGVYKHITGDELG---GHAVKLIGWGTSDDGEDYWLLAN 314
                PV V F V  +F  YK GV+   T         HAV  +G+G  DD   YWL+ N
Sbjct: 296 -----PVSVAFEVVHEFRFYKKGVFTSNTCGNTPMDVNHAVLAVGYGVEDD-VPYWLIKN 348

Query: 315 QWNRGWGDDGYFKIKRGTNECGI 330
            W   WGD+GYFK++ G N CG+
Sbjct: 356 SWGGEWGDNGYFKMEMGKNMCGV 348