Carg23010 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23010
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionReticulon-like protein
LocationCarg_Chr05: 11045243 .. 11049558 (+)
RNA-Seq ExpressionCarg23010
SyntenyCarg23010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCTTCACTTTCCCTTTCAGATTCCCGAGAAAAAGAAAAGGAAAATTCACATCACTCCCACCTCCTCCGCCACTTGCCGCTGTCCATTTGGCTCTGTTCTGTTCTGTTCTGTTCTGTGCTGTTCACCGCCACAGAGATTCTCTCTGTTCTTCTTCTCTCCGCCCTGTTTTGCTGCCACCGCTGCTTTTAAACCTTTAGATTTCTTTGCCGGGGGGTTTTTCTTTTTGGGTTTAGGTAGGCTGCGGCGGCGGAGCGGCAGCGATGGATTTGACCCGGCTGAGGGCGGCAGCGGCGGCGAGGGGGAACGTGGTGGCGGGGTCTGTGTGGGAGAGTAGAATCAGATTGGATGAAGTCAGAGGTGGGGTTAAGGTGTTTAACGGCGACGACGGAAATGCCGAATGTGGGCTTCCGGTTGCTTCTGCCTCCCCCGTCAGCGGCGGCAGTGGAAGGAGGAAGACGTGGAAATCCGATGAGGGTTTTAACTCGATTCTCATCGCTGAGGAGAAACCAGAGTCGTCGAGCAGCGGCGATGAACAGAGTCGTAAAAGCCCGATTCCGGCGCGGAGATTGAGATCCAACGGCAGCCCAATCAAACCCGGCGAGAAAACAGAGAGAAACCCGACGAGGAAGAAGACCGAGCAATCTCGTAAATCTGCCGTCGGGTTAACCAAACCGTCGACGAACGCAGCCGGAAAAACCCCGCCGGAGAAAAATGAATGCAGAGAGAAAGCGATTTCATCTACTCCATCTCAAGATTTCTTCGATGCCTTCGATGAAGAGATTGAGAAAGAAAGCTTCGACGTTAAGGAAATCAATTTACCAGAACGGAAGAAAATCGCAGCAGAAACTTTCCCGAGAAACAAACAGAATCTACAGAGATTAGGTGAGAAAATCCCAAATCCCAAATCCCAAATCCCGATTTTCATTTTCATTTTCGTTTCCCCTAATTCAATTTCTTGAAATCTTCGGTGTTCTTCAGTGGATTTGATAATGTGGAGAGACATTTCAAGATCCGCTCTGGTTTTCGGAGTGGGAAATCTAGTTATAATCCTCTGTTATTTCATGAAGAACATGAATATCAGGTAAAAGAAAATTCCAAATCCGCCATTGTTGAGGGAAAGAAGAAACTGGGTGATTCAGTAAATTCTTCATTTAATGTTTTTGCAGCTTTATCAGCGTTATTTCGCACATGGGTCTTCTTTATCTGGCTGCCATTTTCGTCCATAGCTCCATTTTTGGAAGGTGTGCTCAAATGGGTTCTTCTTTTCTTCACTGATTTTCCATTTGATTTTCTGACTGATTTTCCCGGGAAAATTACAGGAGAAAAGGAATGGATTCAGATGATGAACATTATGCAATTGAAGAAGAAGAAGTGATTCGATTTGCTAAACGACTTGTTCCTTTCCTGAATCAATTGCTGCAAAATCTCAAGGCTCTGTTCATGGGAGATCCTTCTGCAACCATGAAGGTAATTTTCTTTTTTTCTTTTTTTTAATTTTTTTTAAATTTCCAAATTCCATTACATATATAATTATTTAAAAAAAAAATAGCATTTAAAAACTTATGCCAGATTTAATATTTAATAATTTAGAAAAAAACAAAATGAACATCAAAACTTGAAGGCCATTTAATTAAAAAATTGATAGTGTCGGTTTGATTTTTTAAAATTTTTAATAATTAAATATGAGACATTTATAAATTACCAACCGTGAAATATTTTTAAATTTTTAAAAAATTTAAGAAAAAAATATAATTCAACCGAACATTTATGATTTAGATCGAGTAATATGGGTTGGTCGAGATTTTACATTTATTAAGGTAAATGATTTTGGTAAAAAGAATATTATATTAAATAATATTTGTTTGGTAATTTTTGCAGCTGGGTGTAACGCTGTTTGTGTTGGCAAGGTGGGGTAACTTCATTACCCTTTGGAATGTCATCAAATGTGGTAATTTTTTAAAAATTTAATTTAAATTATTTTCAATTTTAAAATTTGACTTAGTTTTTAAAATAATTTTAAAATGTGGTTTATTTATTTATTTATTTATTATTGGATTCTGACAGGATTCATTGGGGTTTTCACACTGCCCAAGTTAATTACCCACGGTAAAATATATCTTCACCGACTTTTTACTTTTTACTTTTTACCGCTCTTTTTGTCGATAAGTCTTGTCGGTGTTGGAATTGGATACGGCCGAGTACAACAAAAACACCGTACACTCTGTGTTTTGGCGCGTCGATTTGTTTGACTCTGAAACTGGTCCCACTCTGACACTATCCTGACCGTCTATTGTCCCTTCACATCACAACTAAAACCCATAATATATTTTTTTTTTGTCGTCACGTGGGGTCCACAGCTTTCTTCACGAGACGTTTTCAGAGAAATTATTTTATTTTTATTTTTTTTTTTGAAAATTTTGGGAAAATAATTAAATGAAATTATTTGTTTTTCTTTGTCGGTGAATTCGTTTCTTCGTTGGGTTTTCGTGTCGGCTGTGTCGGATGGCGTGATGTTTGATCGAATCATCACAACCGTTCGTTTCAAGATCCTTTTTTTTTTGTCCCCTAAATTCTAAAATTAATCACCATTTTTTTTTATTTTTATAAATTTAATTTCGTGTCTATATATTATGTCTAGTAATTCTTTGAAATGTTTAGAATTTTTAGTAAATTTATGAAATTTAATTTTACTTTTAGTGAAATCAATTAAAGACAAATTTAAAAAATTAATGACTAAATTTGCTGTTTATCGGTAACGTTGTCGTTCGTGTTTCGATTTATTGTTAATAGCGTTGAATGGTATTGTTGAAAATATTTAGCTTTTCATTACGGTTTTCCATATATATATATATATATATATCATATTTGACGATTGTTGAAGATATTTGACTTTTCATTACGGTTTACATATGCATCATATTTGACTCTTGATTATAGGCTACTATCGTGTATCCTCTTTCTATTTTCTTTGTGATCTATTTGATTCTGTAAATGATTATAGATAGAGAACTTTCACCACCATTAGGTGTCGGGATTTTAGCAGATATTTTCATGGTATGAGAGCAACAGTTTAGCAACCCCGACCCTTTCTTCCCCATGGCTTTCGAATCCTTTCTCATCTTCTTCCTTTCAACACTCTAATCCGTATGACTCGAGATATGAGCCGAACTTATGCGAGTCGTATACGAATTCGAATCTCTAAGGGTTGACATGTCTCTACCTTAGGGATCCTATGCGGCTGCCTCACCACCATGTGACCTCTCTCTCTTTTAGAGAGTTGTTTGTCCTTCTAACCTAGGGACTACCCTTTGCTTTTTATACTCTATGTCTTTGATATCCATTCATATTGAGTTTGTGTACCTCTTCATTATTAAAGCCTAGTTTGTACAATCATGAAATAATACACTTTAGCCGACGAAAGGGGCTTTGCAACAATAATACCGTCGGTAATTACGAAAGATGCACCAAACCCTAAAGAATAACATAAAACCTAACGAGAGATCGAACTGTAACAGCCCAAGTCCACCGCTAGCAGCTACTGTCCTCTTTGGGGCGCGCTTTTCCTTTCGGGCTAGATAGAGGGTCTAGCCCTAATATTACCAGTGCCTTGCAGTGTCGGGTGACTGACTTTGATACCATCTGTAACAGCTCAAGCCCACTACTAGCAGATACTATTCTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGATATTTAAACGCATAAAAGTTTTTGCACTCGTGTAATGAATGTTTCGTTCCCTTCTCCAACTGATGTGGAATCTCACATTTTGATTTTTGTTGATGCGTTTTAAGAGTTGTAATGATGTCATGGCGTTCTCTTTTGTAAATTTAGGTGAACAATGGTTGACACTTTGTGGGAGTTCTTGGAAGTTATGCTCTCACAAGAAGGCAGTGGCTGTTGCCCTCTTCTTCCTAGTTTGGAGTTTTTCTTCGACTCTATATCGTGTTTGGGCAGGTAACCGTTTCGAACTGAAAACTCGATCATATGAATTTCAAAGCATGGTTTGAACATGGAACAGCCATGCGTAACATGTTTTATATGACGTTGTATGTACGATGTTTGCAGTGTTCTTCGTGTTCGTGAGCTTTCGGTACTACCAAGAGCTTAAAAATGTAGAAGTAGCTCCAAAAAACGACACGGTTTCGGTAAGTTCGAAGCTGAAGAGACAATATTGAACAATTTTGAGGCTGTTTTTCTCAGTTTTTTTTTTTTGAGCTAGTTAGCCAAAATTACCATGTAAATTCATGCACTATGGTAGAAATCAATCCATCGTGTTTTATCGTTACATA

mRNA sequence

CATCTTCACTTTCCCTTTCAGATTCCCGAGAAAAAGAAAAGGAAAATTCACATCACTCCCACCTCCTCCGCCACTTGCCGCTGTCCATTTGGCTCTGTTCTGTTCTGTTCTGTTCTGTGCTGTTCACCGCCACAGAGATTCTCTCTGTTCTTCTTCTCTCCGCCCTGTTTTGCTGCCACCGCTGCTTTTAAACCTTTAGATTTCTTTGCCGGGGGGTTTTTCTTTTTGGGTTTAGGTAGGCTGCGGCGGCGGAGCGGCAGCGATGGATTTGACCCGGCTGAGGGCGGCAGCGGCGGCGAGGGGGAACGTGGTGGCGGGGTCTGTGTGGGAGAGTAGAATCAGATTGGATGAAGTCAGAGGTGGGGTTAAGGTGTTTAACGGCGACGACGGAAATGCCGAATGTGGGCTTCCGGTTGCTTCTGCCTCCCCCGTCAGCGGCGGCAGTGGAAGGAGGAAGACGTGGAAATCCGATGAGGGTTTTAACTCGATTCTCATCGCTGAGGAGAAACCAGAGTCGTCGAGCAGCGGCGATGAACAGAGTCGTAAAAGCCCGATTCCGGCGCGGAGATTGAGATCCAACGGCAGCCCAATCAAACCCGGCGAGAAAACAGAGAGAAACCCGACGAGGAAGAAGACCGAGCAATCTCGTAAATCTGCCGTCGGGTTAACCAAACCGTCGACGAACGCAGCCGGAAAAACCCCGCCGGAGAAAAATGAATGCAGAGAGAAAGCGATTTCATCTACTCCATCTCAAGATTTCTTCGATGCCTTCGATGAAGAGATTGAGAAAGAAAGCTTCGACGTTAAGGAAATCAATTTACCAGAACGGAAGAAAATCGCAGCAGAAACTTTCCCGAGAAACAAACAGAATCTACAGAGATTAGTGGATTTGATAATGTGGAGAGACATTTCAAGATCCGCTCTGGTTTTCGGAGTGGGAAATCTAGTTATAATCCTCTGTTATTTCATGAAGAACATGAATATCAGCTTTATCAGCGTTATTTCGCACATGGGTCTTCTTTATCTGGCTGCCATTTTCGTCCATAGCTCCATTTTTGGAAGGAGAAAAGGAATGGATTCAGATGATGAACATTATGCAATTGAAGAAGAAGAAGTGATTCGATTTGCTAAACGACTTGTTCCTTTCCTGAATCAATTGCTGCAAAATCTCAAGGCTCTGTTCATGGGAGATCCTTCTGCAACCATGAAGCTGGGTGTAACGCTGTTTGTGTTGGCAAGGTGGGGTAACTTCATTACCCTTTGGAATGTCATCAAATGTGGATTCATTGGGGTTTTCACACTGCCCAAGTTAATTACCCACGTGTTCTTCGTGTTCGTGAGCTTTCGGTACTACCAAGAGCTTAAAAATGTAGAAGTAGCTCCAAAAAACGACACGGTTTCGGTAAGTTCGAAGCTGAAGAGACAATATTGAACAATTTTGAGGCTGTTTTTCTCAGTTTTTTTTTTTTGAGCTAGTTAGCCAAAATTACCATGTAAATTCATGCACTATGGTAGAAATCAATCCATCGTGTTTTATCGTTACATA

Coding sequence (CDS)

ATGGATTTGACCCGGCTGAGGGCGGCAGCGGCGGCGAGGGGGAACGTGGTGGCGGGGTCTGTGTGGGAGAGTAGAATCAGATTGGATGAAGTCAGAGGTGGGGTTAAGGTGTTTAACGGCGACGACGGAAATGCCGAATGTGGGCTTCCGGTTGCTTCTGCCTCCCCCGTCAGCGGCGGCAGTGGAAGGAGGAAGACGTGGAAATCCGATGAGGGTTTTAACTCGATTCTCATCGCTGAGGAGAAACCAGAGTCGTCGAGCAGCGGCGATGAACAGAGTCGTAAAAGCCCGATTCCGGCGCGGAGATTGAGATCCAACGGCAGCCCAATCAAACCCGGCGAGAAAACAGAGAGAAACCCGACGAGGAAGAAGACCGAGCAATCTCGTAAATCTGCCGTCGGGTTAACCAAACCGTCGACGAACGCAGCCGGAAAAACCCCGCCGGAGAAAAATGAATGCAGAGAGAAAGCGATTTCATCTACTCCATCTCAAGATTTCTTCGATGCCTTCGATGAAGAGATTGAGAAAGAAAGCTTCGACGTTAAGGAAATCAATTTACCAGAACGGAAGAAAATCGCAGCAGAAACTTTCCCGAGAAACAAACAGAATCTACAGAGATTAGTGGATTTGATAATGTGGAGAGACATTTCAAGATCCGCTCTGGTTTTCGGAGTGGGAAATCTAGTTATAATCCTCTGTTATTTCATGAAGAACATGAATATCAGCTTTATCAGCGTTATTTCGCACATGGGTCTTCTTTATCTGGCTGCCATTTTCGTCCATAGCTCCATTTTTGGAAGGAGAAAAGGAATGGATTCAGATGATGAACATTATGCAATTGAAGAAGAAGAAGTGATTCGATTTGCTAAACGACTTGTTCCTTTCCTGAATCAATTGCTGCAAAATCTCAAGGCTCTGTTCATGGGAGATCCTTCTGCAACCATGAAGCTGGGTGTAACGCTGTTTGTGTTGGCAAGGTGGGGTAACTTCATTACCCTTTGGAATGTCATCAAATGTGGATTCATTGGGGTTTTCACACTGCCCAAGTTAATTACCCACGTGTTCTTCGTGTTCGTGAGCTTTCGGTACTACCAAGAGCTTAAAAATGTAGAAGTAGCTCCAAAAAACGACACGGTTTCGGTAAGTTCGAAGCTGAAGAGACAATATTGA

Protein sequence

MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGGSGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNPTRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFDVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMNISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLLQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITHVFFVFVSFRYYQELKNVEVAPKNDTVSVSSKLKRQY
Homology
BLAST of Carg23010 vs. NCBI nr
Match: KAG7030334.1 (Reticulon-like protein B21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 746.9 bits (1927), Expect = 8.8e-212
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG
Sbjct: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120
           SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP
Sbjct: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120

Query: 121 TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFD 180
           TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFD
Sbjct: 121 TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFD 180

Query: 181 VKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN 240
           VKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN
Sbjct: 181 VKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN 240

Query: 241 ISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLL 300
           ISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLL
Sbjct: 241 ISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLL 300

Query: 301 QNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITHVFFVFVS 360
           QNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITHVFFVFVS
Sbjct: 301 QNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITHVFFVFVS 360

Query: 361 FRYYQELKNVEVAPKNDTVSVSSKLKRQY 390
           FRYYQELKNVEVAPKNDTVSVSSKLKRQY
Sbjct: 361 FRYYQELKNVEVAPKNDTVSVSSKLKRQY 389

BLAST of Carg23010 vs. NCBI nr
Match: KAG6599344.1 (Reticulon-like protein B21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 710.3 bits (1832), Expect = 9.2e-201
Identity = 381/431 (88.40%), Postives = 384/431 (89.10%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG
Sbjct: 40  MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 99

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120
           SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP
Sbjct: 100 SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 159

Query: 121 TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFD-EEIEKESF 180
           TRKKTEQSRKSAVGLTKPSTNA GKTPPEKNECREKAISSTPSQDFFDAFD EEIEKESF
Sbjct: 160 TRKKTEQSRKSAVGLTKPSTNATGKTPPEKNECREKAISSTPSQDFFDAFDEEEIEKESF 219

Query: 181 DVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNM 240
           DVKEINLPERKKI AETFPRNKQNLQRL+DLIMWRDISRSALVFGVGNLVIILCYFMKNM
Sbjct: 220 DVKEINLPERKKIGAETFPRNKQNLQRLLDLIMWRDISRSALVFGVGNLVIILCYFMKNM 279

Query: 241 NISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQL 300
           NISFISVISHMGLLYLAAIFVHSS+FGRRKGMDSDDEHYAIEEEEVIRF KRLVPFLNQL
Sbjct: 280 NISFISVISHMGLLYLAAIFVHSSVFGRRKGMDSDDEHYAIEEEEVIRFTKRLVPFLNQL 339

Query: 301 LQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITH------ 360
           LQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITH      
Sbjct: 340 LQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITHGEQWLT 399

Query: 361 -----------------------------------VFFVFVSFRYYQELKNVEVAPKNDT 390
                                              VFFVFVSFRYYQE+KN EVAPKNDT
Sbjct: 400 LCGSSWKLCSHKKAVAVALFFLVWSFSSTLYRVWAVFFVFVSFRYYQEVKNGEVAPKNDT 459

BLAST of Carg23010 vs. NCBI nr
Match: XP_022946666.1 (reticulon-like protein B21 [Cucurbita moschata])

HSP 1 Score: 706.1 bits (1821), Expect = 1.7e-199
Identity = 380/431 (88.17%), Postives = 383/431 (88.86%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG
Sbjct: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120
           SGRRKTWKSDEGFNSILIAEEKPE SSSGDEQSRKSPIPARRLRSNGSPIKPGEK ERNP
Sbjct: 61  SGRRKTWKSDEGFNSILIAEEKPELSSSGDEQSRKSPIPARRLRSNGSPIKPGEKIERNP 120

Query: 121 TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFD-EEIEKESF 180
           TRKKTEQSRKSAVGLTKPSTN  GKTPPEKNECREKAISSTP QDFFDAFD EEIEKESF
Sbjct: 121 TRKKTEQSRKSAVGLTKPSTNGTGKTPPEKNECREKAISSTPFQDFFDAFDEEEIEKESF 180

Query: 181 DVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNM 240
           DVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNM
Sbjct: 181 DVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNM 240

Query: 241 NISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQL 300
           NISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQL
Sbjct: 241 NISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQL 300

Query: 301 LQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITH------ 360
           LQNLKALFMGDPSATMKLGVTLFVLARWG+FITLWNV+KCGFIGVFTLPKLITH      
Sbjct: 301 LQNLKALFMGDPSATMKLGVTLFVLARWGSFITLWNVVKCGFIGVFTLPKLITHGEQWLT 360

Query: 361 -----------------------------------VFFVFVSFRYYQELKNVEVAPKNDT 390
                                              VFFVFVSFRYYQE+KN EVAPKNDT
Sbjct: 361 LCGSSWKLCSHKKAVAVALFFVVWSFSSTLYRVWAVFFVFVSFRYYQEVKNGEVAPKNDT 420

BLAST of Carg23010 vs. NCBI nr
Match: XP_022999357.1 (reticulon-like protein B21 [Cucurbita maxima])

HSP 1 Score: 696.4 bits (1796), Expect = 1.4e-196
Identity = 374/431 (86.77%), Postives = 382/431 (88.63%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLP ASASPVSGG
Sbjct: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPAASASPVSGG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120
           SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP
Sbjct: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120

Query: 121 TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFD 180
            RKKT+QSRKSAVGLTKPSTN  GKTPPEKNECREKAISS PSQDFFDAFDEEIEKESFD
Sbjct: 121 MRKKTDQSRKSAVGLTKPSTNGTGKTPPEKNECREKAISSPPSQDFFDAFDEEIEKESFD 180

Query: 181 VKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN 240
           VKEINLPERKKIAAETFPRNKQ LQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN
Sbjct: 181 VKEINLPERKKIAAETFPRNKQILQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN 240

Query: 241 ISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLL 300
           ISFISVISHMGLLYLAAIFVHSSIFGRRKG+DSDDEHYAIEEEEVIRFAKRLVPF+NQLL
Sbjct: 241 ISFISVISHMGLLYLAAIFVHSSIFGRRKGIDSDDEHYAIEEEEVIRFAKRLVPFVNQLL 300

Query: 301 QNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITH------- 360
           QNLKALFMGDPSATMKLGVTLFVLARWG+FITLWNV+KCGFIGVFTLPKLITH       
Sbjct: 301 QNLKALFMGDPSATMKLGVTLFVLARWGSFITLWNVVKCGFIGVFTLPKLITHGEQWLTL 360

Query: 361 ----------------------------------VFFVFVSFRYYQELKNVEVAPKNDTV 390
                                             VFFVFVSF+YYQE+KN EVAPKND+V
Sbjct: 361 CGSSWKLCSHKKAVAVALFFIGWSFSSTLYRVWAVFFVFVSFQYYQEVKNGEVAPKNDSV 420

BLAST of Carg23010 vs. NCBI nr
Match: XP_023546680.1 (reticulon-like protein B21 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546681.1 reticulon-like protein B21 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 666.0 bits (1717), Expect = 2.0e-187
Identity = 355/405 (87.65%), Postives = 361/405 (89.14%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLP ASASPVSGG
Sbjct: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPAASASPVSGG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120
           SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP
Sbjct: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120

Query: 121 TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFD 180
           TRKK+EQSRKSAVGLTKPSTNA GKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFD
Sbjct: 121 TRKKSEQSRKSAVGLTKPSTNATGKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFD 180

Query: 181 VKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN 240
           VKEINLPERKKIAAETFPRNKQNLQRL+DLIMWRDISRSALVFGVGNLVIILCYFMKNMN
Sbjct: 181 VKEINLPERKKIAAETFPRNKQNLQRLMDLIMWRDISRSALVFGVGNLVIILCYFMKNMN 240

Query: 241 ISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLL 300
           ISFISVISHMGLLYLAAIFVHSSIFGRRKG+DSDDEHYAIEEEEVIRFAKRLVPF+NQLL
Sbjct: 241 ISFISVISHMGLLYLAAIFVHSSIFGRRKGIDSDDEHYAIEEEEVIRFAKRLVPFVNQLL 300

Query: 301 QNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITH------- 360
            NLKALFMGDPSATMKLGVTLFVLARWG+FITLWNV+KCGFIGVFTLPKLITH       
Sbjct: 301 LNLKALFMGDPSATMKLGVTLFVLARWGSFITLWNVVKCGFIGVFTLPKLITHGEQWLTL 360

Query: 361 ----------------------------------VFFVFVSFRYY 365
                                             VFFVFVSFRYY
Sbjct: 361 CGSSWKLCSHKKAVAVALFFIVWSFSSTLYRVWAVFFVFVSFRYY 405

BLAST of Carg23010 vs. ExPASy Swiss-Prot
Match: Q56X72 (Reticulon-like protein B21 OS=Arabidopsis thaliana OX=3702 GN=RTNLB21 PE=2 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 5.1e-37
Identity = 79/158 (50.00%), Postives = 111/158 (70.25%), Query Frame = 0

Query: 195 ETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMNISFISVISHMGLLY 254
           + F  ++  LQ LVDL+MWRD+SRS LVFG G  +II   +  ++N SFISV+++MGL+Y
Sbjct: 218 DNFTHSQSKLQSLVDLVMWRDVSRSTLVFGFGTFLIISSSYANDLNFSFISVVAYMGLIY 277

Query: 255 LAAIFVHSSIFGRRKGMDSDDEH--YAIEEEEVIRFAKRLVPFLNQLLQNLKALFMGDPS 314
           L  +FV  S+  R  GM  ++ H    + EE+V R  + ++P+LN+ L  L+ALF GDPS
Sbjct: 278 LGLMFVLKSLIHR--GMVEEERHKVVGVREEDVKRMLRLIMPYLNESLHQLRALFSGDPS 337

Query: 315 ATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKL 351
            T+K+GV LFVLAR G+ ITLWN+ K GF+G FT+PK+
Sbjct: 338 TTLKMGVVLFVLARCGSSITLWNLAKFGFLGAFTIPKI 373

BLAST of Carg23010 vs. ExPASy Swiss-Prot
Match: Q6DR04 (Reticulon-like protein B17 OS=Arabidopsis thaliana OX=3702 GN=RTNLB17 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.8e-19
Identity = 51/142 (35.92%), Postives = 84/142 (59.15%), Query Frame = 0

Query: 209 DLIMWRDISRSALVFGVGNLVIILCYFMKNMNISFISVISHMGLLYLAAIFVHSSIFGRR 268
           DL+MWRD+++S L FG G L  +   F K +N S  S +S++GL+ L   F+ +++  R+
Sbjct: 170 DLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLCGSFLSNTLCQRK 229

Query: 269 KGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLLQNLKALFMGDPSATMKLGVTLFVLARWG 328
              +     + + E++V+R A+R++P  N  +     LF G+PS T+K+   L + A +G
Sbjct: 230 N--EDTKRAFHVSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKVTPFLLLGAEYG 289

Query: 329 NFITLWNVIKCGFIGVFTLPKL 351
           + ITLW +   GF   FT+PKL
Sbjct: 290 HLITLWRLSAFGFFLSFTIPKL 309

BLAST of Carg23010 vs. ExPASy Swiss-Prot
Match: Q8LDS3 (Reticulon-like protein B18 OS=Arabidopsis thaliana OX=3702 GN=RTNLB18 PE=2 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-15
Identity = 72/276 (26.09%), Postives = 134/276 (48.55%), Query Frame = 0

Query: 78  IAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNPTRKKTEQSRKSAVGLTK 137
           + + +   S+  D ++ ++ + ARR ++ G   + G     +P   +  + R  A+  TK
Sbjct: 76  LRKSRTRLSNRFDMEAAEAAVTARRSKTKGG--QNGLLASPSPRNFRRSRLRSEAMVDTK 135

Query: 138 PSTNAAGKTPPEK--NECREKAISSTPSQDFFDAFDEEIEKESFDVKEINLPERKKIAAE 197
            +T        EK  N+ ++K +  +  +             S D       + + +   
Sbjct: 136 ENTEPIVVVTDEKKQNQRKQKKLGRSKKEKHSSVPLLASPSPSSD-------QPQDVCQG 195

Query: 198 TFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVII-LCYFMKNMNISFISVISHMGLLY 257
              R ++N+    DLIMWRD+++S L FG G +  +  C+  K  N S  S IS++GLL+
Sbjct: 196 DLERIRENIS---DLIMWRDVAKSTLWFGFGCICFLSTCFAAKGFNFSVFSAISYLGLLF 255

Query: 258 LAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLLQNLKALFMGDPSAT 317
           L   F+ +++  R++  +       + E++V+R A+R++P  N  +     LF G+P+ T
Sbjct: 256 LGVSFLSNTL--RQRVTEEARRELKLSEDDVLRIARRMLPITNLAISKTSELFSGEPAMT 315

Query: 318 MKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKL 351
           +K+   + + A +G  ITLW +   GF   FT+PKL
Sbjct: 316 LKVAPFVLMGAEYGYLITLWRLCAFGFFLSFTIPKL 337

BLAST of Carg23010 vs. ExPASy TrEMBL
Match: A0A6J1G4A6 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450663 PE=4 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 8.4e-200
Identity = 380/431 (88.17%), Postives = 383/431 (88.86%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG
Sbjct: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120
           SGRRKTWKSDEGFNSILIAEEKPE SSSGDEQSRKSPIPARRLRSNGSPIKPGEK ERNP
Sbjct: 61  SGRRKTWKSDEGFNSILIAEEKPELSSSGDEQSRKSPIPARRLRSNGSPIKPGEKIERNP 120

Query: 121 TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFD-EEIEKESF 180
           TRKKTEQSRKSAVGLTKPSTN  GKTPPEKNECREKAISSTP QDFFDAFD EEIEKESF
Sbjct: 121 TRKKTEQSRKSAVGLTKPSTNGTGKTPPEKNECREKAISSTPFQDFFDAFDEEEIEKESF 180

Query: 181 DVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNM 240
           DVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNM
Sbjct: 181 DVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNM 240

Query: 241 NISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQL 300
           NISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQL
Sbjct: 241 NISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQL 300

Query: 301 LQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITH------ 360
           LQNLKALFMGDPSATMKLGVTLFVLARWG+FITLWNV+KCGFIGVFTLPKLITH      
Sbjct: 301 LQNLKALFMGDPSATMKLGVTLFVLARWGSFITLWNVVKCGFIGVFTLPKLITHGEQWLT 360

Query: 361 -----------------------------------VFFVFVSFRYYQELKNVEVAPKNDT 390
                                              VFFVFVSFRYYQE+KN EVAPKNDT
Sbjct: 361 LCGSSWKLCSHKKAVAVALFFVVWSFSSTLYRVWAVFFVFVSFRYYQEVKNGEVAPKNDT 420

BLAST of Carg23010 vs. ExPASy TrEMBL
Match: A0A6J1KAN6 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493754 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 6.6e-197
Identity = 374/431 (86.77%), Postives = 382/431 (88.63%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLP ASASPVSGG
Sbjct: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPAASASPVSGG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120
           SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP
Sbjct: 61  SGRRKTWKSDEGFNSILIAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNP 120

Query: 121 TRKKTEQSRKSAVGLTKPSTNAAGKTPPEKNECREKAISSTPSQDFFDAFDEEIEKESFD 180
            RKKT+QSRKSAVGLTKPSTN  GKTPPEKNECREKAISS PSQDFFDAFDEEIEKESFD
Sbjct: 121 MRKKTDQSRKSAVGLTKPSTNGTGKTPPEKNECREKAISSPPSQDFFDAFDEEIEKESFD 180

Query: 181 VKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN 240
           VKEINLPERKKIAAETFPRNKQ LQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN
Sbjct: 181 VKEINLPERKKIAAETFPRNKQILQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMN 240

Query: 241 ISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLL 300
           ISFISVISHMGLLYLAAIFVHSSIFGRRKG+DSDDEHYAIEEEEVIRFAKRLVPF+NQLL
Sbjct: 241 ISFISVISHMGLLYLAAIFVHSSIFGRRKGIDSDDEHYAIEEEEVIRFAKRLVPFVNQLL 300

Query: 301 QNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKLITH------- 360
           QNLKALFMGDPSATMKLGVTLFVLARWG+FITLWNV+KCGFIGVFTLPKLITH       
Sbjct: 301 QNLKALFMGDPSATMKLGVTLFVLARWGSFITLWNVVKCGFIGVFTLPKLITHGEQWLTL 360

Query: 361 ----------------------------------VFFVFVSFRYYQELKNVEVAPKNDTV 390
                                             VFFVFVSF+YYQE+KN EVAPKND+V
Sbjct: 361 CGSSWKLCSHKKAVAVALFFIGWSFSSTLYRVWAVFFVFVSFQYYQEVKNGEVAPKNDSV 420

BLAST of Carg23010 vs. ExPASy TrEMBL
Match: A0A5A7V8P0 (Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001050 PE=4 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.7e-158
Identity = 317/447 (70.92%), Postives = 347/447 (77.63%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDL+R RAA AARGNVVAGSVWESRIR DEVRGG+KVFNGDD NAECG+P  +AS  +GG
Sbjct: 1   MDLSRRRAAVAARGNVVAGSVWESRIRFDEVRGGIKVFNGDDENAECGVPTTAASAAAGG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESS-SSGDEQSRKSPIPARRLRSNGSPIKP----GEK 120
           SGRRKTWKSDEGFN ILIAEEKPESS SSGDEQSRKSP P+RRLRSNGSP KP    GEK
Sbjct: 61  SGRRKTWKSDEGFNPILIAEEKPESSPSSGDEQSRKSPTPSRRLRSNGSPNKPVQVSGEK 120

Query: 121 TERNPTRKKTEQSRKSAVGLTKPSTNAAGKTPPEK-----NECREKAISSTPSQDFFDAF 180
           TERN TRKKT+QSRKS V LTKP TN  GKT  EK     NEC+EK ISS+ SQDFFDAF
Sbjct: 121 TERNSTRKKTDQSRKSVVALTKPPTNGIGKTSSEKSFKELNECKEKVISSSTSQDFFDAF 180

Query: 181 DEEIEKESFDVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVI 240
           +EEIEKESFDVKEINLPERKKI A+TFP NKQ LQ L+DLIMWRD SRS LVFGVGNLVI
Sbjct: 181 EEEIEKESFDVKEINLPERKKIIAQTFPGNKQKLQTLMDLIMWRDFSRSGLVFGVGNLVI 240

Query: 241 ILCYFMKNMNISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAK 300
           IL  F+KN+NISFIS+ISHMGLLYL AIFVHSSIFGRRK +DSDDE++ +EEE++IRFAK
Sbjct: 241 ILSCFIKNINISFISLISHMGLLYLTAIFVHSSIFGRRKRIDSDDENFVVEEEDMIRFAK 300

Query: 301 RLVPFLNQLLQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKL 360
           RLVPF+N+LLQNLKALF GD SATMK+GV LFVLA+WG+FITLWNV+K GFIGVFTLPKL
Sbjct: 301 RLVPFVNELLQNLKALFRGDSSATMKVGVLLFVLAKWGSFITLWNVLKIGFIGVFTLPKL 360

Query: 361 ITH-----------------------------------------VFFVFVSFRYYQE--- 390
           ITH                                          F V VSFRYYQE   
Sbjct: 361 ITHGEHWLKLCGSSWKLCSHKRGVFVAIFFLVWNFSSTLYRVWAAFIVLVSFRYYQESPE 420

BLAST of Carg23010 vs. ExPASy TrEMBL
Match: A0A1S3C3D4 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103496431 PE=4 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.7e-158
Identity = 317/447 (70.92%), Postives = 347/447 (77.63%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDL+R RAA AARGNVVAGSVWESRIR DEVRGG+KVFNGDD NAECG+P  +AS  +GG
Sbjct: 1   MDLSRRRAAVAARGNVVAGSVWESRIRFDEVRGGIKVFNGDDENAECGVPTTAASAAAGG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESS-SSGDEQSRKSPIPARRLRSNGSPIKP----GEK 120
           SGRRKTWKSDEGFN ILIAEEKPESS SSGDEQSRKSP P+RRLRSNGSP KP    GEK
Sbjct: 61  SGRRKTWKSDEGFNPILIAEEKPESSPSSGDEQSRKSPTPSRRLRSNGSPNKPVQVSGEK 120

Query: 121 TERNPTRKKTEQSRKSAVGLTKPSTNAAGKTPPEK-----NECREKAISSTPSQDFFDAF 180
           TERN TRKKT+QSRKS VGLTKP TN  GKT  EK     NEC+EK ISS+ SQDFFDAF
Sbjct: 121 TERNSTRKKTDQSRKSVVGLTKPPTNGIGKTSSEKSFKELNECKEKVISSSTSQDFFDAF 180

Query: 181 DEEIEKESFDVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVI 240
           +EEIEKESFDVKEINLPERKKI A+TFP NKQ LQ L+DLIMWRD SRS LVFGVGNLVI
Sbjct: 181 EEEIEKESFDVKEINLPERKKIIAQTFPGNKQKLQTLMDLIMWRDFSRSGLVFGVGNLVI 240

Query: 241 ILCYFMKNMNISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAK 300
           IL  F+KN+NISFIS+ISHMGLLYL AIFVHSSIFGRRK +DSDDE++ +EEE++IRFAK
Sbjct: 241 ILSCFIKNINISFISLISHMGLLYLTAIFVHSSIFGRRKRIDSDDENFVVEEEDMIRFAK 300

Query: 301 RLVPFLNQLLQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKL 360
           R VPF+N+LLQNLKALF GD SATMK+GV LFVLA+WG+FITLWNV+K GFIGVFTLPKL
Sbjct: 301 RSVPFVNELLQNLKALFRGDSSATMKVGVLLFVLAKWGSFITLWNVLKIGFIGVFTLPKL 360

Query: 361 ITH-----------------------------------------VFFVFVSFRYYQE--- 390
           ITH                                          F V VSFRYYQE   
Sbjct: 361 ITHGEHWLKLCGSSWKLCSHKRGVFVAIFFLVWNFSSTLYRVWAAFIVLVSFRYYQESPE 420

BLAST of Carg23010 vs. ExPASy TrEMBL
Match: A0A0A0LG57 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G009500 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 4.5e-153
Identity = 311/447 (69.57%), Postives = 341/447 (76.29%), Query Frame = 0

Query: 1   MDLTRLRAAAAARGNVVAGSVWESRIRLDEVRGGVKVFNGDDGNAECGLPVASASPVSGG 60
           MDL+R RAA AA+GNVVAGSVWESRIR DEVRGG+KVFNGDD N ECG+P  +A   S G
Sbjct: 1   MDLSRRRAAVAAKGNVVAGSVWESRIRFDEVRGGIKVFNGDDENVECGIPTTAA---SAG 60

Query: 61  SGRRKTWKSDEGFNSILIAEEKPESS-SSGDEQSRKSPIPARRLRSNGSPIKP----GEK 120
           SGRRKTWKSDEGFN ILIAEEKPESS SSGDEQSRKSP P+RRLRSN SP KP    GEK
Sbjct: 61  SGRRKTWKSDEGFNPILIAEEKPESSPSSGDEQSRKSPTPSRRLRSNSSPNKPVQVSGEK 120

Query: 121 TERNPTRKKTEQSRKSAVGLTKPSTNAAGKTPPEK-----NECREKAISSTPSQDFFDAF 180
            ERN TRKKT+QSRKSAVGLTKP TN  GKT  EK     NECREK ISS+ SQDFFDAF
Sbjct: 121 IERNSTRKKTDQSRKSAVGLTKPPTNGIGKTSSEKSFKESNECREKVISSSTSQDFFDAF 180

Query: 181 DEEIEKESFDVKEINLPERKKIAAETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVI 240
           +EEIEKESFDVKEINLPERKKI A+TFP NKQ LQ L+DLIMWRD SRS LVFGVGNLVI
Sbjct: 181 EEEIEKESFDVKEINLPERKKIIAQTFPGNKQKLQTLMDLIMWRDFSRSGLVFGVGNLVI 240

Query: 241 ILCYFMKNMNISFISVISHMGLLYLAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAK 300
           IL  F+KN+NIS IS+ISHMGLLYL  IFVHSSIFGRRK +DS+DE+  +EEE++IRFAK
Sbjct: 241 ILSCFIKNINISLISLISHMGLLYLTTIFVHSSIFGRRKRIDSNDENLVVEEEDMIRFAK 300

Query: 301 RLVPFLNQLLQNLKALFMGDPSATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKL 360
           RLVPF+N+LLQNLKALF GDPSATMK+GV LFVLA+WG+FITLWNV+K GFIGVFTLPKL
Sbjct: 301 RLVPFVNELLQNLKALFRGDPSATMKVGVLLFVLAKWGSFITLWNVLKIGFIGVFTLPKL 360

Query: 361 ITH-----------------------------------------VFFVFVSFRYYQE--- 390
           ITH                                          F V VSFRYYQE   
Sbjct: 361 ITHGEHWLKLCGSSWKLCSHKRGVFIAIFFLVWNFSSTLYRVWAAFIVLVSFRYYQESPE 420

BLAST of Carg23010 vs. TAIR 10
Match: AT5G58000.1 (Reticulon family protein )

HSP 1 Score: 156.8 bits (395), Expect = 3.6e-38
Identity = 79/158 (50.00%), Postives = 111/158 (70.25%), Query Frame = 0

Query: 195 ETFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVIILCYFMKNMNISFISVISHMGLLY 254
           + F  ++  LQ LVDL+MWRD+SRS LVFG G  +II   +  ++N SFISV+++MGL+Y
Sbjct: 218 DNFTHSQSKLQSLVDLVMWRDVSRSTLVFGFGTFLIISSSYANDLNFSFISVVAYMGLIY 277

Query: 255 LAAIFVHSSIFGRRKGMDSDDEH--YAIEEEEVIRFAKRLVPFLNQLLQNLKALFMGDPS 314
           L  +FV  S+  R  GM  ++ H    + EE+V R  + ++P+LN+ L  L+ALF GDPS
Sbjct: 278 LGLMFVLKSLIHR--GMVEEERHKVVGVREEDVKRMLRLIMPYLNESLHQLRALFSGDPS 337

Query: 315 ATMKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKL 351
            T+K+GV LFVLAR G+ ITLWN+ K GF+G FT+PK+
Sbjct: 338 TTLKMGVVLFVLARCGSSITLWNLAKFGFLGAFTIPKI 373

BLAST of Carg23010 vs. TAIR 10
Match: AT2G20590.1 (Reticulon family protein )

HSP 1 Score: 97.1 bits (240), Expect = 3.4e-20
Identity = 51/142 (35.92%), Postives = 84/142 (59.15%), Query Frame = 0

Query: 209 DLIMWRDISRSALVFGVGNLVIILCYFMKNMNISFISVISHMGLLYLAAIFVHSSIFGRR 268
           DL+MWRD+++S L FG G L  +   F K +N S  S +S++GL+ L   F+ +++  R+
Sbjct: 170 DLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLCGSFLSNTLCQRK 229

Query: 269 KGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLLQNLKALFMGDPSATMKLGVTLFVLARWG 328
              +     + + E++V+R A+R++P  N  +     LF G+PS T+K+   L + A +G
Sbjct: 230 N--EDTKRAFHVSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKVTPFLLLGAEYG 289

Query: 329 NFITLWNVIKCGFIGVFTLPKL 351
           + ITLW +   GF   FT+PKL
Sbjct: 290 HLITLWRLSAFGFFLSFTIPKL 309

BLAST of Carg23010 vs. TAIR 10
Match: AT2G20590.2 (Reticulon family protein )

HSP 1 Score: 97.1 bits (240), Expect = 3.4e-20
Identity = 51/142 (35.92%), Postives = 84/142 (59.15%), Query Frame = 0

Query: 209 DLIMWRDISRSALVFGVGNLVIILCYFMKNMNISFISVISHMGLLYLAAIFVHSSIFGRR 268
           DL+MWRD+++S L FG G L  +   F K +N S  S +S++GL+ L   F+ +++  R+
Sbjct: 170 DLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLCGSFLSNTLCQRK 229

Query: 269 KGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLLQNLKALFMGDPSATMKLGVTLFVLARWG 328
              +     + + E++V+R A+R++P  N  +     LF G+PS T+K+   L + A +G
Sbjct: 230 N--EDTKRAFHVSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKVTPFLLLGAEYG 289

Query: 329 NFITLWNVIKCGFIGVFTLPKL 351
           + ITLW +   GF   FT+PKL
Sbjct: 290 HLITLWRLSAFGFFLSFTIPKL 309

BLAST of Carg23010 vs. TAIR 10
Match: AT4G28430.1 (Reticulon family protein )

HSP 1 Score: 85.9 bits (211), Expect = 7.9e-17
Identity = 72/276 (26.09%), Postives = 134/276 (48.55%), Query Frame = 0

Query: 78  IAEEKPESSSSGDEQSRKSPIPARRLRSNGSPIKPGEKTERNPTRKKTEQSRKSAVGLTK 137
           + + +   S+  D ++ ++ + ARR ++ G   + G     +P   +  + R  A+  TK
Sbjct: 76  LRKSRTRLSNRFDMEAAEAAVTARRSKTKGG--QNGLLASPSPRNFRRSRLRSEAMVDTK 135

Query: 138 PSTNAAGKTPPEK--NECREKAISSTPSQDFFDAFDEEIEKESFDVKEINLPERKKIAAE 197
            +T        EK  N+ ++K +  +  +             S D       + + +   
Sbjct: 136 ENTEPIVVVTDEKKQNQRKQKKLGRSKKEKHSSVPLLASPSPSSD-------QPQDVCQG 195

Query: 198 TFPRNKQNLQRLVDLIMWRDISRSALVFGVGNLVII-LCYFMKNMNISFISVISHMGLLY 257
              R ++N+    DLIMWRD+++S L FG G +  +  C+  K  N S  S IS++GLL+
Sbjct: 196 DLERIRENIS---DLIMWRDVAKSTLWFGFGCICFLSTCFAAKGFNFSVFSAISYLGLLF 255

Query: 258 LAAIFVHSSIFGRRKGMDSDDEHYAIEEEEVIRFAKRLVPFLNQLLQNLKALFMGDPSAT 317
           L   F+ +++  R++  +       + E++V+R A+R++P  N  +     LF G+P+ T
Sbjct: 256 LGVSFLSNTL--RQRVTEEARRELKLSEDDVLRIARRMLPITNLAISKTSELFSGEPAMT 315

Query: 318 MKLGVTLFVLARWGNFITLWNVIKCGFIGVFTLPKL 351
           +K+   + + A +G  ITLW +   GF   FT+PKL
Sbjct: 316 LKVAPFVLMGAEYGYLITLWRLCAFGFFLSFTIPKL 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030334.18.8e-212100.00Reticulon-like protein B21 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6599344.19.2e-20188.40Reticulon-like protein B21, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022946666.11.7e-19988.17reticulon-like protein B21 [Cucurbita moschata][more]
XP_022999357.11.4e-19686.77reticulon-like protein B21 [Cucurbita maxima][more]
XP_023546680.12.0e-18787.65reticulon-like protein B21 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546681... [more]
Match NameE-valueIdentityDescription
Q56X725.1e-3750.00Reticulon-like protein B21 OS=Arabidopsis thaliana OX=3702 GN=RTNLB21 PE=2 SV=2[more]
Q6DR044.8e-1935.92Reticulon-like protein B17 OS=Arabidopsis thaliana OX=3702 GN=RTNLB17 PE=2 SV=1[more]
Q8LDS31.1e-1526.09Reticulon-like protein B18 OS=Arabidopsis thaliana OX=3702 GN=RTNLB18 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1G4A68.4e-20088.17Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450663 PE=4 SV=1[more]
A0A6J1KAN66.6e-19786.77Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493754 PE=4 SV=1[more]
A0A5A7V8P02.7e-15870.92Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A1S3C3D42.7e-15870.92Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103496431 PE=4 SV=1[more]
A0A0A0LG574.5e-15369.57Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G009500 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G58000.13.6e-3850.00Reticulon family protein [more]
AT2G20590.13.4e-2035.92Reticulon family protein [more]
AT2G20590.23.4e-2035.92Reticulon family protein [more]
AT4G28430.17.9e-1726.09Reticulon family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003388ReticulonPFAMPF02453Reticuloncoord: 208..350
e-value: 1.4E-26
score: 93.3
IPR003388ReticulonPROSITEPS50845RETICULONcoord: 207..350
score: 16.121819
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..130
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..146
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..158
NoneNo IPR availablePANTHERPTHR46626:SF1RETICULON-LIKE PROTEIN B21coord: 1..191
NoneNo IPR availablePANTHERPTHR46626:SF1RETICULON-LIKE PROTEIN B21coord: 194..352
IPR044647Reticulon-like protein B17/18/21PANTHERPTHR46626RETICULON-LIKE PROTEIN B17coord: 1..191
IPR044647Reticulon-like protein B17/18/21PANTHERPTHR46626RETICULON-LIKE PROTEIN B17coord: 194..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23010-RACarg23010-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane