Carg23006 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23006
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionB3 domain-containing protein At2g31720-like
LocationCarg_Chr05: 11023589 .. 11024308 (+)
RNA-Seq ExpressionCarg23006
SyntenyCarg23006
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGATAATCTAGACTCCATCAGCTCTAGCCCGTTTCATTGGATGGACAATTTCAACCAAATAGTTTGTAGAAAGAAGAGGACTCTAGTTGAATATATCGACTGTCCTGGCCATGAGAACCCGAACGTGTCGCCGACGCCGATGAACAGAGATGATCCAGAAGCCGGAAATCTGTCTGGGGTCGTGAACGGCGATAGCCCAATTGCCGAAATCCCACCGGAGCCGATGAATGAAGATGACCCAGATGAGGAAATTCAGCCGGTGGCGGAGGAGGAGATGCCGGCGGAGATGAGAGAACATATAACGGAAAGAATGGGTGGTTACCGTGTGCAAATGATAATCGAAAAGAAGTTGACGGCGACTGACATGAGGACGAATCAAGGGCGGCTGTCGCTGCCGAAGAAGATGGTGAGAGTGGAATTTATGACGGCGGAGGAGGAGAAGATGTTGGAGCACCGGAGCAAAGGGGACGATGAATTGAACTATATAGACACATTCATAGTGGGATCCGATTTAAAAGTGAATCCAATTCGGTTCAAGAAGTGGCTAATGGGGAAAAATTACGTGAATTGTTTGGTAAACAAATGGAACTCTTTTGCTCGAGCTTATGGACTCAAGACCGGAACTAAAGTTCGTGTTTGGAGTTTCAGGGTTGATGAAAGGGACGAGAACGACGTTATTAAATCTGAATTGCGCTTCGCCCTTGTCAAGATTTAA

mRNA sequence

ATGTCTGATAATCTAGACTCCATCAGCTCTAGCCCGTTTCATTGGATGGACAATTTCAACCAAATAGTTTGTAGAAAGAAGAGGACTCTAGTTGAATATATCGACTGTCCTGGCCATGAGAACCCGAACGTGTCGCCGACGCCGATGAACAGAGATGATCCAGAAGCCGGAAATCTGTCTGGGGTCGTGAACGGCGATAGCCCAATTGCCGAAATCCCACCGGAGCCGATGAATGAAGATGACCCAGATGAGGAAATTCAGCCGGTGGCGGAGGAGGAGATGCCGGCGGAGATGAGAGAACATATAACGGAAAGAATGGGTGGTTACCGTGTGCAAATGATAATCGAAAAGAAGTTGACGGCGACTGACATGAGGACGAATCAAGGGCGGCTGTCGCTGCCGAAGAAGATGGTGAGAGTGGAATTTATGACGGCGGAGGAGGAGAAGATGTTGGAGCACCGGAGCAAAGGGGACGATGAATTGAACTATATAGACACATTCATAGTGGGATCCGATTTAAAAGTGAATCCAATTCGGTTCAAGAAGTGGCTAATGGGGAAAAATTACGTGAATTGTTTGGTAAACAAATGGAACTCTTTTGCTCGAGCTTATGGACTCAAGACCGGAACTAAAGTTCGTGTTTGGAGTTTCAGGGTTGATGAAAGGGACGAGAACGACGTTATTAAATCTGAATTGCGCTTCGCCCTTGTCAAGATTTAA

Coding sequence (CDS)

ATGTCTGATAATCTAGACTCCATCAGCTCTAGCCCGTTTCATTGGATGGACAATTTCAACCAAATAGTTTGTAGAAAGAAGAGGACTCTAGTTGAATATATCGACTGTCCTGGCCATGAGAACCCGAACGTGTCGCCGACGCCGATGAACAGAGATGATCCAGAAGCCGGAAATCTGTCTGGGGTCGTGAACGGCGATAGCCCAATTGCCGAAATCCCACCGGAGCCGATGAATGAAGATGACCCAGATGAGGAAATTCAGCCGGTGGCGGAGGAGGAGATGCCGGCGGAGATGAGAGAACATATAACGGAAAGAATGGGTGGTTACCGTGTGCAAATGATAATCGAAAAGAAGTTGACGGCGACTGACATGAGGACGAATCAAGGGCGGCTGTCGCTGCCGAAGAAGATGGTGAGAGTGGAATTTATGACGGCGGAGGAGGAGAAGATGTTGGAGCACCGGAGCAAAGGGGACGATGAATTGAACTATATAGACACATTCATAGTGGGATCCGATTTAAAAGTGAATCCAATTCGGTTCAAGAAGTGGCTAATGGGGAAAAATTACGTGAATTGTTTGGTAAACAAATGGAACTCTTTTGCTCGAGCTTATGGACTCAAGACCGGAACTAAAGTTCGTGTTTGGAGTTTCAGGGTTGATGAAAGGGACGAGAACGACGTTATTAAATCTGAATTGCGCTTCGCCCTTGTCAAGATTTAA

Protein sequence

MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLSGVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLTATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRFKKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI
Homology
BLAST of Carg23006 vs. NCBI nr
Match: XP_022946870.1 (B3 domain-containing protein At2g31720-like [Cucurbita moschata] >KAG7030330.1 B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 485.3 bits (1248), Expect = 2.9e-133
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60
           MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS
Sbjct: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60

Query: 61  GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120
           GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT
Sbjct: 61  GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120

Query: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180
           ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF
Sbjct: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180

Query: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI 240
           KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI
Sbjct: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI 239

BLAST of Carg23006 vs. NCBI nr
Match: KAG6599340.1 (B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 481.5 bits (1238), Expect = 4.3e-132
Identity = 237/239 (99.16%), Postives = 238/239 (99.58%), Query Frame = 0

Query: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60
           MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS
Sbjct: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60

Query: 61  GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120
           GVVN DSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT
Sbjct: 61  GVVNVDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120

Query: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180
           ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF
Sbjct: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180

Query: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI 240
           KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDV+KSELRFALVKI
Sbjct: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVVKSELRFALVKI 239

BLAST of Carg23006 vs. NCBI nr
Match: XP_023545398.1 (B3 domain-containing protein At2g31720-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461.5 bits (1186), Expect = 4.6e-126
Identity = 228/239 (95.40%), Postives = 233/239 (97.49%), Query Frame = 0

Query: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60
           MSDNLDSISSS F WMDNFNQIVCRKKRTLVEYIDCPGHENPN+SPTPMNR DPEA N S
Sbjct: 1   MSDNLDSISSSSFQWMDNFNQIVCRKKRTLVEYIDCPGHENPNMSPTPMNRVDPEAENRS 60

Query: 61  GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120
           GVVNG+SPIAE PPEPMNEDDPDEEIQPVAEEEMPAEMR+HITERMGGYRVQMIIEKKLT
Sbjct: 61  GVVNGNSPIAENPPEPMNEDDPDEEIQPVAEEEMPAEMRDHITERMGGYRVQMIIEKKLT 120

Query: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180
           ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKG+DELNYIDTFIVGSDLKVNPIRF
Sbjct: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGEDELNYIDTFIVGSDLKVNPIRF 180

Query: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI 240
           KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDV+KSELRFALVKI
Sbjct: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVVKSELRFALVKI 239

BLAST of Carg23006 vs. NCBI nr
Match: XP_022998957.1 (B3 domain-containing protein At2g31720-like [Cucurbita maxima])

HSP 1 Score: 461.1 bits (1185), Expect = 5.9e-126
Identity = 227/239 (94.98%), Postives = 233/239 (97.49%), Query Frame = 0

Query: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60
           MSDNL+SISS+ F WMDNFNQIVCRKKRTLVEYIDC GHENPN+SPTPMNR DPEAGN S
Sbjct: 1   MSDNLNSISSNSFQWMDNFNQIVCRKKRTLVEYIDCSGHENPNMSPTPMNRVDPEAGNWS 60

Query: 61  GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120
           GVVNG+SPIAEIPPEPMNEDDPDEEIQPV EEEMPAEMREHITERMGGYRVQMIIEKKLT
Sbjct: 61  GVVNGNSPIAEIPPEPMNEDDPDEEIQPVVEEEMPAEMREHITERMGGYRVQMIIEKKLT 120

Query: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180
           ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDL+VNPIRF
Sbjct: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLRVNPIRF 180

Query: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI 240
           KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDV+KSELRFALVKI
Sbjct: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVVKSELRFALVKI 239

BLAST of Carg23006 vs. NCBI nr
Match: XP_022146365.1 (putative B3 domain-containing protein At2g27410 [Momordica charantia])

HSP 1 Score: 114.8 bits (286), Expect = 1.0e-21
Identity = 68/177 (38.42%), Postives = 104/177 (58.76%), Query Frame = 0

Query: 68  PIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLTATDMRTN 127
           P+++  PE        + ++     EMPA MR+ I E +GGY +Q++I+K+L  TDM  N
Sbjct: 96  PVSKSEPEMKQRKKKRKAMELPVTPEMPAAMRDRIVE-IGGYGIQLVIQKQLQNTDMNKN 155

Query: 128 QGRLSLPKKMVRV-EFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRFKKWLMG 187
            GRLSLP K +R+ +F+T EE K+L   S  +++   +D  I+  DLK   I  KKW +G
Sbjct: 156 HGRLSLPAKKLRIHDFVTEEERKLLNQHSNNNNKKG-MDVPIIDPDLKERRICLKKWKIG 215

Query: 188 KNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDEND----VIKSELRFALVKI 240
              + CL+ +WNSF     L++G  +++WSFR DE DE +    +  S L  A+VK+
Sbjct: 216 SGDLYCLMTQWNSFVEETALRSGDFIQLWSFRKDEFDEAEETEKISTSRLWLAMVKL 270

BLAST of Carg23006 vs. ExPASy Swiss-Prot
Match: Q5RM09 (B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05920 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-07
Identity = 46/168 (27.38%), Postives = 81/168 (48.21%), Query Frame = 0

Query: 76  PMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLTATDMRTNQGRLSLP- 135
           P  +     E  P    + P  + + ++   G    ++IIEK L + D+   Q RLS+P 
Sbjct: 145 PRRKKTTSYEFVPPKHPQEPQWLLQVMSRMNGAGDPKLIIEKNLDSNDVDPRQNRLSIPI 204

Query: 136 KKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRFKKWLM----GKNYV 195
             +++ +F+T +E ++++     ++    +  F+V    K   + FK+W M    G +Y 
Sbjct: 205 NTVIQNDFLTLDESRLIDEDEITNEGNMGVAAFLVDQRTKKWNMGFKQWFMTTDSGSSYW 264

Query: 196 N-CLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALV 238
           +  L  +W++     GLK G K+ +WSFR      ND+    L FALV
Sbjct: 265 SFVLRGEWSNVVETNGLKEGDKISLWSFR-----SNDI----LCFALV 303

BLAST of Carg23006 vs. ExPASy Swiss-Prot
Match: Q9SCJ8 (Putative B3 domain-containing protein At3g49610 OS=Arabidopsis thaliana OX=3702 GN=At3g49610 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.7e-06
Identity = 35/118 (29.66%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 111 VQMIIEKKLTATDMRTNQGRLSLP-KKMVRVEFMTAEEEKML---EHRSKGDDELNYIDT 170
           +++I EK LTATD++ ++ RL +P  K++R +F+T EE + +   +   + D +   + T
Sbjct: 205 IKLIYEKTLTATDVKPSESRLLIPFNKLLRNDFLTPEESRAIAIDKEEEEEDTKKIGVKT 264

Query: 171 FIVGSDLKVNPIRFKKWLM-----GKNYVNCLVNK-WNSFARAYGLKTGTKVRVWSFR 219
            IV    K   +RF  W+M     G   +   +N+ WN       LK    + +W+FR
Sbjct: 265 IIVNQFSKEWSLRFLIWVMKKKKSGNGTLYYTLNRGWNGVVSGNKLKANDNISLWTFR 322

BLAST of Carg23006 vs. ExPASy Swiss-Prot
Match: Q9FFX1 (B3 domain-containing protein At5g38500 OS=Arabidopsis thaliana OX=3702 GN=At5g38500 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 6.4e-06
Identity = 37/135 (27.41%), Postives = 64/135 (47.41%), Query Frame = 0

Query: 112 QMIIEKKLTATDMRTNQGRLSLP-KKMVRVEFMTAEE-EKMLEHRSKGDDELNY------ 171
           ++I E+ L  +D+ +N  RL +P +K++R +F+T EE   M E + K D++++       
Sbjct: 265 KLIFERTLFKSDVNSNLSRLLIPFQKLIRNDFLTPEECRAMQEDKDKDDEDISVGTILVC 324

Query: 172 ---------------IDTFIVGSDLKVNPIRFKKWLM----GKNYVNCLVN-KWNSFARA 219
                            T +V    K+  +RFK W M    G   +N ++N  WN   + 
Sbjct: 325 QAKQEDEDKDDEDIGAGTILVNQRFKMWGLRFKIWGMEKDSGHGTLNYILNWDWNDVVKG 384

BLAST of Carg23006 vs. ExPASy Swiss-Prot
Match: Q9SIC7 (B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31420 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 52/208 (25.00%), Postives = 94/208 (45.19%), Query Frame = 0

Query: 16  MDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLSGVVNGDSPIAEIPPE 75
           ++ F+QIV + K+ ++  +D P       SP+  N+ D     +  +   DS      PE
Sbjct: 15  LEEFSQIVLKGKKKII--LDIP-------SPSQQNKSD-----IVSLCPADS------PE 74

Query: 76  PMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKK-LTATDMRTNQGRLSLP 135
             N   PD  ++ + +E               GY  ++II+++ L  TD+R NQG LS+ 
Sbjct: 75  TCNSSIPDWLVKVMRKE--------------NGYDPKLIIKRRVLCTTDLRKNQGCLSMH 134

Query: 136 -KKMVRVEFMTAEEEKMLEH---RSKGDDELNYIDTFIVGSDLKVNPIRFKKWLMGKNYV 195
             K+ + +F+T +E + L+    ++K D     +  F+V  +   + +  KKW     + 
Sbjct: 135 LSKLEKSDFLTEDETRFLDEDFLKAKRDG----LKVFLVDPESDKHVVYLKKWNGNTVWK 184

Query: 196 NCLVNKWNSFARAYGLKTGTKVRVWSFR 219
             L + WN+       K    + +WSFR
Sbjct: 195 YVLSHGWNNVIDKKIFKVNDVIEIWSFR 184

BLAST of Carg23006 vs. ExPASy Swiss-Prot
Match: Q9ZR14 (Putative B3 domain-containing protein At4g03170 OS=Arabidopsis thaliana OX=3702 GN=At4g03170 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 5.4e-05
Identity = 36/105 (34.29%), Postives = 50/105 (47.62%), Query Frame = 0

Query: 115 IEKKLTATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLK 174
           I+K+L ++D+  +Q  L L K+ V+        EKML      ++ +  ID  + G D K
Sbjct: 136 IKKQLMSSDVDKDQCMLMLSKEQVK--------EKMLPFLEDSENPVKGIDVSVYGPDGK 195

Query: 175 VNPIRFKKWLMGKNYVNCLVNKWNSFARAYGLK-TGTKVRVWSFR 219
           V  + FK W   K  V  L + W  F   YGL  T   V VW FR
Sbjct: 196 VQQMEFKMWNGDKTPV--LTSGWKQFVEDYGLSMTCDFVTVWMFR 230

BLAST of Carg23006 vs. ExPASy TrEMBL
Match: A0A6J1G587 (B3 domain-containing protein At2g31720-like OS=Cucurbita moschata OX=3662 GN=LOC111450806 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.4e-133
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60
           MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS
Sbjct: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60

Query: 61  GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120
           GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT
Sbjct: 61  GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120

Query: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180
           ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF
Sbjct: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180

Query: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI 240
           KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI
Sbjct: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI 239

BLAST of Carg23006 vs. ExPASy TrEMBL
Match: A0A6J1K9G9 (B3 domain-containing protein At2g31720-like OS=Cucurbita maxima OX=3661 GN=LOC111493483 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 2.9e-126
Identity = 227/239 (94.98%), Postives = 233/239 (97.49%), Query Frame = 0

Query: 1   MSDNLDSISSSPFHWMDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLS 60
           MSDNL+SISS+ F WMDNFNQIVCRKKRTLVEYIDC GHENPN+SPTPMNR DPEAGN S
Sbjct: 1   MSDNLNSISSNSFQWMDNFNQIVCRKKRTLVEYIDCSGHENPNMSPTPMNRVDPEAGNWS 60

Query: 61  GVVNGDSPIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLT 120
           GVVNG+SPIAEIPPEPMNEDDPDEEIQPV EEEMPAEMREHITERMGGYRVQMIIEKKLT
Sbjct: 61  GVVNGNSPIAEIPPEPMNEDDPDEEIQPVVEEEMPAEMREHITERMGGYRVQMIIEKKLT 120

Query: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRF 180
           ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDL+VNPIRF
Sbjct: 121 ATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLRVNPIRF 180

Query: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALVKI 240
           KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDV+KSELRFALVKI
Sbjct: 181 KKWLMGKNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVVKSELRFALVKI 239

BLAST of Carg23006 vs. ExPASy TrEMBL
Match: A0A6J1CX35 (putative B3 domain-containing protein At2g27410 OS=Momordica charantia OX=3673 GN=LOC111015593 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 5.1e-22
Identity = 68/177 (38.42%), Postives = 104/177 (58.76%), Query Frame = 0

Query: 68  PIAEIPPEPMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLTATDMRTN 127
           P+++  PE        + ++     EMPA MR+ I E +GGY +Q++I+K+L  TDM  N
Sbjct: 96  PVSKSEPEMKQRKKKRKAMELPVTPEMPAAMRDRIVE-IGGYGIQLVIQKQLQNTDMNKN 155

Query: 128 QGRLSLPKKMVRV-EFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRFKKWLMG 187
            GRLSLP K +R+ +F+T EE K+L   S  +++   +D  I+  DLK   I  KKW +G
Sbjct: 156 HGRLSLPAKKLRIHDFVTEEERKLLNQHSNNNNKKG-MDVPIIDPDLKERRICLKKWKIG 215

Query: 188 KNYVNCLVNKWNSFARAYGLKTGTKVRVWSFRVDERDEND----VIKSELRFALVKI 240
              + CL+ +WNSF     L++G  +++WSFR DE DE +    +  S L  A+VK+
Sbjct: 216 SGDLYCLMTQWNSFVEETALRSGDFIQLWSFRKDEFDEAEETEKISTSRLWLAMVKL 270

BLAST of Carg23006 vs. ExPASy TrEMBL
Match: A0A0A0KP87 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G544060 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 4.7e-20
Identity = 61/147 (41.50%), Postives = 85/147 (57.82%), Query Frame = 0

Query: 94  MPAEMREHITERMGGYRVQMIIEKKLTATDMRTNQGRLSLPKKMVRVEFMTAEEEKML-E 153
           MP  MR+ I E MGGY + ++I+K+LT TD+  N GRLS+  K +  +F T EE K+L E
Sbjct: 124 MPVAMRDRILE-MGGYEINLVIQKQLTDTDLNKNHGRLSMNTKQLSFDFATEEESKLLSE 183

Query: 154 HRSKGDDELNYIDTFIVGSDLKVNPIRFKKWLMGKNYVNCLVNKWNSFARAYGLKTGTKV 213
             +K    +N     I+   L+   +  KKW +G  YV CL+ KWN      GLK+G ++
Sbjct: 184 QENKNKMGIN---VMILDDVLEERMLCLKKWKIGSGYVYCLMTKWNLMVEERGLKSGEEI 243

Query: 214 RVWSFRVDERDENDVIKSELRFALVKI 240
           +VWSFR D+ DE       L  ALVK+
Sbjct: 244 QVWSFRKDDEDE----AHRLCLALVKL 262

BLAST of Carg23006 vs. ExPASy TrEMBL
Match: A0A6J1JKC5 (B3 domain-containing protein At2g32645-like OS=Cucurbita maxima OX=3661 GN=LOC111487692 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 8.1e-20
Identity = 60/148 (40.54%), Postives = 89/148 (60.14%), Query Frame = 0

Query: 93  EMPAEMREHITERMGGYRVQMIIEKKLTATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLE 152
           +MPA MR+ I E MG Y++Q+ I+K+L  TDM  N GRLS P K ++V+F T EE ++L+
Sbjct: 93  DMPAAMRDRIGE-MGAYQIQLAIQKQLQDTDMNKNHGRLSFPAKKLKVDFATEEERRVLK 152

Query: 153 HRSKGDDELNYIDTFIVGSDLKVNPIRFKKWLMGKNYVNCLVNKWNSFARAYGLKTGTKV 212
            +  G  +   ++  IV   L+ + I  K W +G     CL+ +WNS     G K+G  V
Sbjct: 153 QQENGGKK--GMNVMIVDPLLRESSICLKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHV 212

Query: 213 RVWSFRVDERD-ENDVIKSELRFALVKI 240
           ++WSFR DE + E   + S L FA+VK+
Sbjct: 213 QLWSFRKDEFEAETQTMVSRLCFAMVKL 237

BLAST of Carg23006 vs. TAIR 10
Match: AT1G05920.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 57.8 bits (138), Expect = 1.4e-08
Identity = 46/168 (27.38%), Postives = 81/168 (48.21%), Query Frame = 0

Query: 76  PMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKKLTATDMRTNQGRLSLP- 135
           P  +     E  P    + P  + + ++   G    ++IIEK L + D+   Q RLS+P 
Sbjct: 145 PRRKKTTSYEFVPPKHPQEPQWLLQVMSRMNGAGDPKLIIEKNLDSNDVDPRQNRLSIPI 204

Query: 136 KKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLKVNPIRFKKWLM----GKNYV 195
             +++ +F+T +E ++++     ++    +  F+V    K   + FK+W M    G +Y 
Sbjct: 205 NTVIQNDFLTLDESRLIDEDEITNEGNMGVAAFLVDQRTKKWNMGFKQWFMTTDSGSSYW 264

Query: 196 N-CLVNKWNSFARAYGLKTGTKVRVWSFRVDERDENDVIKSELRFALV 238
           +  L  +W++     GLK G K+ +WSFR      ND+    L FALV
Sbjct: 265 SFVLRGEWSNVVETNGLKEGDKISLWSFR-----SNDI----LCFALV 303

BLAST of Carg23006 vs. TAIR 10
Match: AT3G49610.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 54.7 bits (130), Expect = 1.2e-07
Identity = 35/118 (29.66%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 111 VQMIIEKKLTATDMRTNQGRLSLP-KKMVRVEFMTAEEEKML---EHRSKGDDELNYIDT 170
           +++I EK LTATD++ ++ RL +P  K++R +F+T EE + +   +   + D +   + T
Sbjct: 205 IKLIYEKTLTATDVKPSESRLLIPFNKLLRNDFLTPEESRAIAIDKEEEEEDTKKIGVKT 264

Query: 171 FIVGSDLKVNPIRFKKWLM-----GKNYVNCLVNK-WNSFARAYGLKTGTKVRVWSFR 219
            IV    K   +RF  W+M     G   +   +N+ WN       LK    + +W+FR
Sbjct: 265 IIVNQFSKEWSLRFLIWVMKKKKSGNGTLYYTLNRGWNGVVSGNKLKANDNISLWTFR 322

BLAST of Carg23006 vs. TAIR 10
Match: AT5G38500.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 52.8 bits (125), Expect = 4.5e-07
Identity = 37/135 (27.41%), Postives = 64/135 (47.41%), Query Frame = 0

Query: 112 QMIIEKKLTATDMRTNQGRLSLP-KKMVRVEFMTAEE-EKMLEHRSKGDDELNY------ 171
           ++I E+ L  +D+ +N  RL +P +K++R +F+T EE   M E + K D++++       
Sbjct: 265 KLIFERTLFKSDVNSNLSRLLIPFQKLIRNDFLTPEECRAMQEDKDKDDEDISVGTILVC 324

Query: 172 ---------------IDTFIVGSDLKVNPIRFKKWLM----GKNYVNCLVN-KWNSFARA 219
                            T +V    K+  +RFK W M    G   +N ++N  WN   + 
Sbjct: 325 QAKQEDEDKDDEDIGAGTILVNQRFKMWGLRFKIWGMEKDSGHGTLNYILNWDWNDVVKG 384

BLAST of Carg23006 vs. TAIR 10
Match: AT2G31420.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 52.0 bits (123), Expect = 7.8e-07
Identity = 52/208 (25.00%), Postives = 94/208 (45.19%), Query Frame = 0

Query: 16  MDNFNQIVCRKKRTLVEYIDCPGHENPNVSPTPMNRDDPEAGNLSGVVNGDSPIAEIPPE 75
           ++ F+QIV + K+ ++  +D P       SP+  N+ D     +  +   DS      PE
Sbjct: 15  LEEFSQIVLKGKKKII--LDIP-------SPSQQNKSD-----IVSLCPADS------PE 74

Query: 76  PMNEDDPDEEIQPVAEEEMPAEMREHITERMGGYRVQMIIEKK-LTATDMRTNQGRLSLP 135
             N   PD  ++ + +E               GY  ++II+++ L  TD+R NQG LS+ 
Sbjct: 75  TCNSSIPDWLVKVMRKE--------------NGYDPKLIIKRRVLCTTDLRKNQGCLSMH 134

Query: 136 -KKMVRVEFMTAEEEKMLEH---RSKGDDELNYIDTFIVGSDLKVNPIRFKKWLMGKNYV 195
             K+ + +F+T +E + L+    ++K D     +  F+V  +   + +  KKW     + 
Sbjct: 135 LSKLEKSDFLTEDETRFLDEDFLKAKRDG----LKVFLVDPESDKHVVYLKKWNGNTVWK 184

Query: 196 NCLVNKWNSFARAYGLKTGTKVRVWSFR 219
             L + WN+       K    + +WSFR
Sbjct: 195 YVLSHGWNNVIDKKIFKVNDVIEIWSFR 184

BLAST of Carg23006 vs. TAIR 10
Match: AT4G03170.1 (AP2/B3-like transcriptional factor family protein )

HSP 1 Score: 49.7 bits (117), Expect = 3.8e-06
Identity = 36/105 (34.29%), Postives = 50/105 (47.62%), Query Frame = 0

Query: 115 IEKKLTATDMRTNQGRLSLPKKMVRVEFMTAEEEKMLEHRSKGDDELNYIDTFIVGSDLK 174
           I+K+L ++D+  +Q  L L K+ V+        EKML      ++ +  ID  + G D K
Sbjct: 136 IKKQLMSSDVDKDQCMLMLSKEQVK--------EKMLPFLEDSENPVKGIDVSVYGPDGK 195

Query: 175 VNPIRFKKWLMGKNYVNCLVNKWNSFARAYGLK-TGTKVRVWSFR 219
           V  + FK W   K  V  L + W  F   YGL  T   V VW FR
Sbjct: 196 VQQMEFKMWNGDKTPV--LTSGWKQFVEDYGLSMTCDFVTVWMFR 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022946870.12.9e-133100.00B3 domain-containing protein At2g31720-like [Cucurbita moschata] >KAG7030330.1 B... [more]
KAG6599340.14.3e-13299.16B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023545398.14.6e-12695.40B3 domain-containing protein At2g31720-like [Cucurbita pepo subsp. pepo][more]
XP_022998957.15.9e-12694.98B3 domain-containing protein At2g31720-like [Cucurbita maxima][more]
XP_022146365.11.0e-2138.42putative B3 domain-containing protein At2g27410 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q5RM092.0e-0727.38B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05... [more]
Q9SCJ81.7e-0629.66Putative B3 domain-containing protein At3g49610 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9FFX16.4e-0627.41B3 domain-containing protein At5g38500 OS=Arabidopsis thaliana OX=3702 GN=At5g38... [more]
Q9SIC71.1e-0525.00B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31... [more]
Q9ZR145.4e-0534.29Putative B3 domain-containing protein At4g03170 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1G5871.4e-133100.00B3 domain-containing protein At2g31720-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1K9G92.9e-12694.98B3 domain-containing protein At2g31720-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1CX355.1e-2238.42putative B3 domain-containing protein At2g27410 OS=Momordica charantia OX=3673 G... [more]
A0A0A0KP874.7e-2041.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G544060 PE=4 SV=1[more]
A0A6J1JKC58.1e-2040.54B3 domain-containing protein At2g32645-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G05920.11.4e-0827.38Domain of unknown function (DUF313) [more]
AT3G49610.11.2e-0729.66Domain of unknown function (DUF313) [more]
AT5G38500.14.5e-0727.41Domain of unknown function (DUF313) [more]
AT2G31420.17.8e-0725.00Domain of unknown function (DUF313) [more]
AT4G03170.13.8e-0634.29AP2/B3-like transcriptional factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005508B3 domain-containing protein At2g31720-likePFAMPF03754DUF313coord: 93..199
e-value: 5.1E-16
score: 58.9
IPR005508B3 domain-containing protein At2g31720-likePANTHERPTHR31541B3 DOMAIN PLANT PROTEIN-RELATEDcoord: 88..239
IPR015300DNA-binding pseudobarrel domain superfamilyGENE3D2.40.330.10coord: 108..231
e-value: 1.2E-12
score: 49.6
IPR015300DNA-binding pseudobarrel domain superfamilySUPERFAMILY101936DNA-binding pseudobarrel domaincoord: 114..220
NoneNo IPR availablePANTHERPTHR31541:SF25DOMAIN PROTEIN, PUTATIVE (DUF313)-RELATEDcoord: 88..239

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23006-RACarg23006-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding