Carg22896 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22896
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein of unknown function (DUF1666)
LocationCarg_Chr11: 5363749 .. 5366960 (+)
RNA-Seq ExpressionCarg22896
SyntenyCarg22896
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCGATGAAGAAAGAGGAAGGTAAGACAAAGCCCTCAAGAACTGATGAACGAAACCAAACCACATAACCAAAAATTTCCATCATCCCTTCATCTTAAGCAATCCCCAAACTTCTAATTTTTCTCCTAAAAAACTCCAAAGATTCGTTCAAATCCTTCGCAGAAGAGTCGCTCAACGGTTGTTTTCTGTAAATTTATCCTCCTTTTAGGTCCCATTGGTGATATGTCCCCTTCTTCTTCTTCTTTCCCACTTAATTTCAAACTGGATATCACAGTTTTGATGACCCTTTTTCAGAAATCCATGGATTTGATCTTTTAATGGCTGCTTCTTCTCTTGCTTCTCTGTTCCTTCTTCCCCTGTTTTTGTTTTCTCTGGTTATGGCGTCTTTCAATCATTTCTCTCTCTTTCTTTCTCTCTCCTCTCTCTTTTTCTCTCTTTTTAGCTTCCTCTTCAGGTGGGTTCTTTGAGATTGTGTCCTGTTCTTCATTTTCTTAAAAAAAAAGTTTTTTTTTTCTTTTTTTAATCATGGGGTTTTCGGATTTTCATAGGCCTCTTGATGATGGGAGGAATATGGAAATTTCTGATGTTGTTCTTGTTGATGATGGTGGTGGTTGTGATGATTTACAGCCGCCGGAGAAGGGGACCAGCGGCGGTGGAGCTCCTGAGGTTTCAGATGAAAAGGGAACGAATGAGTTTGTCTTCAGTTTCAGGTTTCAAACTTACGAAGAATTTACCAAATCAAATAAGAAAAATTTGGGTTGTGAGAGATTGGATTCATTGAGTAACAGATATGAGTTTTTGCCGGAGAAGAGTACGAGCCACTTCGTGGAAGAACCTGAAATTCCAAGCTTCACGGTGGAGGTTTTGAATTCTTGTTCGAATTACGAGATTCCCAGAACAGGGGATTTCTCCGTTCGTGAATTTTCCGGGAAAGTTTTGGATTCTCAAAGGATTACAGTGAGCTATGAAGTTTCCGGCGAAATTCCAGAATCTGAAGTCATCGGAGAAACGGCAGAGCTTAGCTCTACGAAGTCCAGAGAAGAACAAATGGCAGAGAAACAGAGTGAACTGAAACAGAGAGATGAAAGAGGAACAGATTTTGTGTCCGATTATGATATGAATTTGAAGATGGGTGGGTACGAGCCGGACGAGGAAACCAACGAAGAATTGGAGAAGTGGGGGGAGGAGGAGGAGGAGGAGGAGTTGAATGGATTGGAAACAGAGTGGGAACATCAAGAACTGATAGAACAGTTGAAAATGGAATTGAAGAAAGTGAGGGCGTCGGGGCTGCCCACCATTTCTGAAGAATCAGAGTCGCCAAAGATAATGGAAGAATTGAAGCCATGGAAGATTGATGAAAGGTTTAAACGTGGAGATTTAATGGAGGAGCTTCATAGTTTCTACAGAAGTTACAGAGAGCGCATGAGGAAATTGGACATCTTGAATTACCAGAAGATGTATGCAATGGGTGAGTTCTTGCTGTTCTTGCTGTTCTTGCTGTTCTTGCTGTTCTTTAAATTTAATTTTGAATTTGATTTCCCCTGTTTTGGTTACAAAATTTGTGATTTTGAATGATGGGTATGTTTTACCTTAGTTTGGTTGAACAAATTAGAGAGAAAGAGGGGGTTGGTTTCACACTCAAAAAAATTTCATTTCTTTTTTAAATTTGGGGTTGGAAGTTTCTTAGAGAGGAGAAAAGTTCCCACTTTTTTATTTTTTTTATTTTGGAACTTTGATTGGATGGAGAAAGTTCTTGAACTTTTTAAAAGGGGTTAGAGAGAGAATTTGGACGTTTGAGCAAAAGATAGATACACGAATGAAACAAGACTTCTGGTTGTAAGGATCTAATGATTAGCACATTCTTCTGTTATAGCTTAATCATAGATACTTCATAGTTAAGTGTGCTTAGTTTTAGAGCAATTCTATGTTTGGTGAGTTCGTAGTGATATCAAAGCATGTTAAAATGACTCGGGTTGGTGTGTAGTGATAATTTCCACAGATTGCATAGTCATTAGATGTTAGTCGAAATTCATAACACTGAATCATTTCGTCCATTTTTGACAAAATTTGTTTGAAATATGATAGGGGTTTTGCAATCGAAGGACCCACTAAAGTCATTTTCTAGCAACACCAAATCTTCATCCCCATCCATCACATCTGTTCTAAGGCTATACAGACAAAAGAAATGTCAAGTGGACCCAATGAAGGATTTCATAAGAGAAGTTCATTGTGATTTGGAGATGGTTTATGTGGCTCAAATGTGTCTTTCTTGGGAGTTCATTCAATGGCAATATGGAAAGGCTTTGGATTTGTGGGAATCTGAGCCTCATGGGCTTCACCATTACAATGAAGTTGCTGGTGAGTTCCAACACTTTCAAGTTCTCTTGGAAAGATTCTTGGAGAATGAAGCTTTTGAGGGCCCGAGAGTTGAGAATTACGTCAAGCAACGATGCGTTGTTCGTAATCTTCTTCAAGTTCCAGTCATCAGAGGTATCTATCTTCAATTCTCTTACCTTCTTCAAGTTCCAGTCATCAGAGGTATCTATCTTCAATTCTCTTACGTATATTTCTATTTCTATGGATTCATCAGCTAATTTGGTTCCTTTTCTCATAGAGGATAAAACGAGGGATCGGAGGAAAGCAAGACAATGGAGAGATCACGAGAATGAAGCAATCACGAACGATATGCTTGTAGAGATCTTGCAAGAATCCATAAGAGTTATCTCGCAATTCATTCGAGCAGATAAAGTTCACAATCCCACCGCAACCCTAAAGCGCCCGAAAAAGTTCCAAGTTGAACTTCAAGACCCTACAGACATGCAACTTCTAACAGAGATCCAAGTTGATCTCCAAAAGGTTCGTTTTTGTTTTAGTTAGATCATTCAAGTCTACGTTTTTGAAACTTAAAGCCTCTAATGTCGATCGGTTTTGTTTTGATGTTGTGGTATTGCAGAAAGAGAGGAAGGTAAAGGAAAAAATGAGGAGTGGGCATTGCATCCTAAAGAAGTTGAAGAAAAATGAAGAAGAAGAAGAAACAGAAGAGCAGTGAGTTTCTTCAGTGAAATGGATATGAAATTGGTTGGAAGAGTACTAAAAATGTCAAGAATAACAACAGATCAATTGATTTGGTGTAGGAACAAATTGAGTAGAATTCATTTCATAAACACAAAAATACATTTAGAACCTTCTTTCTTTCTTTTTCCCTGTTGA

mRNA sequence

ATGTCCGATGAAGAAAGAGGAAGGCCTCTTGATGATGGGAGGAATATGGAAATTTCTGATGTTGTTCTTGTTGATGATGGTGGTGGTTGTGATGATTTACAGCCGCCGGAGAAGGGGACCAGCGGCGGTGGAGCTCCTGAGGTTTCAGATGAAAAGGGAACGAATGAGTTTGTCTTCAGTTTCAGGTTTCAAACTTACGAAGAATTTACCAAATCAAATAAGAAAAATTTGGGTTGTGAGAGATTGGATTCATTGAGTAACAGATATGAGTTTTTGCCGGAGAAGAGTACGAGCCACTTCGTGGAAGAACCTGAAATTCCAAGCTTCACGGTGGAGGTTTTGAATTCTTGTTCGAATTACGAGATTCCCAGAACAGGGGATTTCTCCGTTCGTGAATTTTCCGGGAAAGTTTTGGATTCTCAAAGGATTACAGTGAGCTATGAAGTTTCCGGCGAAATTCCAGAATCTGAAGTCATCGGAGAAACGGCAGAGCTTAGCTCTACGAAGTCCAGAGAAGAACAAATGGCAGAGAAACAGAGTGAACTGAAACAGAGAGATGAAAGAGGAACAGATTTTGTGTCCGATTATGATATGAATTTGAAGATGGGTGGGTACGAGCCGGACGAGGAAACCAACGAAGAATTGGAGAAGTGGGGGGAGGAGGAGGAGGAGGAGGAGTTGAATGGATTGGAAACAGAGTGGGAACATCAAGAACTGATAGAACAGTTGAAAATGGAATTGAAGAAAGTGAGGGCGTCGGGGCTGCCCACCATTTCTGAAGAATCAGAGTCGCCAAAGATAATGGAAGAATTGAAGCCATGGAAGATTGATGAAAGGTTTAAACGTGGAGATTTAATGGAGGAGCTTCATAGTTTCTACAGAAGTTACAGAGAGCGCATGAGGAAATTGGACATCTTGAATTACCAGAAGATGTATGCAATGGGGGTTTTGCAATCGAAGGACCCACTAAAGTCATTTTCTAGCAACACCAAATCTTCATCCCCATCCATCACATCTGTTCTAAGGCTATACAGACAAAAGAAATGTCAAGTGGACCCAATGAAGGATTTCATAAGAGAAGTTCATTGTGATTTGGAGATGGTTTATGTGGCTCAAATGTGTCTTTCTTGGGAGTTCATTCAATGGCAATATGGAAAGGCTTTGGATTTGTGGGAATCTGAGCCTCATGGGCTTCACCATTACAATGAAGTTGCTGGTGAGTTCCAACACTTTCAAGTTCTCTTGGAAAGATTCTTGGAGAATGAAGCTTTTGAGGGCCCGAGAGTTGAGAATTACGTCAAGCAACGATGCGTTGTTCGTAATCTTCTTCAAGTTCCAGTCATCAGAGCTAATTTGGTTCCTTTTCTCATAGAGGATAAAACGAGGGATCGGAGGAAAGCAAGACAATGGAGAGATCACGAGAATGAAGCAATCACGAACGATATGCTTGTAGAGATCTTGCAAGAATCCATAAGAGTTATCTCGCAATTCATTCGAGCAGATAAAGTTCACAATCCCACCGCAACCCTAAAGCGCCCGAAAAAGTTCCAAGTTGAACTTCAAGACCCTACAGACATGCAACTTCTAACAGAGATCCAAGTTGATCTCCAAAAGAAAGAGAGGAAGGTAAAGGAAAAAATGAGGAGTGGGCATTGCATCCTAAAGAAGTTGAAGAAAAATGAAGAAGAAGAAGAAACAGAAGAGCATGAAATGGATATGAAATTGGTTGGAAGAGTACTAAAAATGTCAAGAATAACAACAGATCAATTGATTTGGTGTAGGAACAAATTGAGTAGAATTCATTTCATAAACACAAAAATACATTTAGAACCTTCTTTCTTTCTTTTTCCCTGTTGA

Coding sequence (CDS)

ATGTCCGATGAAGAAAGAGGAAGGCCTCTTGATGATGGGAGGAATATGGAAATTTCTGATGTTGTTCTTGTTGATGATGGTGGTGGTTGTGATGATTTACAGCCGCCGGAGAAGGGGACCAGCGGCGGTGGAGCTCCTGAGGTTTCAGATGAAAAGGGAACGAATGAGTTTGTCTTCAGTTTCAGGTTTCAAACTTACGAAGAATTTACCAAATCAAATAAGAAAAATTTGGGTTGTGAGAGATTGGATTCATTGAGTAACAGATATGAGTTTTTGCCGGAGAAGAGTACGAGCCACTTCGTGGAAGAACCTGAAATTCCAAGCTTCACGGTGGAGGTTTTGAATTCTTGTTCGAATTACGAGATTCCCAGAACAGGGGATTTCTCCGTTCGTGAATTTTCCGGGAAAGTTTTGGATTCTCAAAGGATTACAGTGAGCTATGAAGTTTCCGGCGAAATTCCAGAATCTGAAGTCATCGGAGAAACGGCAGAGCTTAGCTCTACGAAGTCCAGAGAAGAACAAATGGCAGAGAAACAGAGTGAACTGAAACAGAGAGATGAAAGAGGAACAGATTTTGTGTCCGATTATGATATGAATTTGAAGATGGGTGGGTACGAGCCGGACGAGGAAACCAACGAAGAATTGGAGAAGTGGGGGGAGGAGGAGGAGGAGGAGGAGTTGAATGGATTGGAAACAGAGTGGGAACATCAAGAACTGATAGAACAGTTGAAAATGGAATTGAAGAAAGTGAGGGCGTCGGGGCTGCCCACCATTTCTGAAGAATCAGAGTCGCCAAAGATAATGGAAGAATTGAAGCCATGGAAGATTGATGAAAGGTTTAAACGTGGAGATTTAATGGAGGAGCTTCATAGTTTCTACAGAAGTTACAGAGAGCGCATGAGGAAATTGGACATCTTGAATTACCAGAAGATGTATGCAATGGGGGTTTTGCAATCGAAGGACCCACTAAAGTCATTTTCTAGCAACACCAAATCTTCATCCCCATCCATCACATCTGTTCTAAGGCTATACAGACAAAAGAAATGTCAAGTGGACCCAATGAAGGATTTCATAAGAGAAGTTCATTGTGATTTGGAGATGGTTTATGTGGCTCAAATGTGTCTTTCTTGGGAGTTCATTCAATGGCAATATGGAAAGGCTTTGGATTTGTGGGAATCTGAGCCTCATGGGCTTCACCATTACAATGAAGTTGCTGGTGAGTTCCAACACTTTCAAGTTCTCTTGGAAAGATTCTTGGAGAATGAAGCTTTTGAGGGCCCGAGAGTTGAGAATTACGTCAAGCAACGATGCGTTGTTCGTAATCTTCTTCAAGTTCCAGTCATCAGAGCTAATTTGGTTCCTTTTCTCATAGAGGATAAAACGAGGGATCGGAGGAAAGCAAGACAATGGAGAGATCACGAGAATGAAGCAATCACGAACGATATGCTTGTAGAGATCTTGCAAGAATCCATAAGAGTTATCTCGCAATTCATTCGAGCAGATAAAGTTCACAATCCCACCGCAACCCTAAAGCGCCCGAAAAAGTTCCAAGTTGAACTTCAAGACCCTACAGACATGCAACTTCTAACAGAGATCCAAGTTGATCTCCAAAAGAAAGAGAGGAAGGTAAAGGAAAAAATGAGGAGTGGGCATTGCATCCTAAAGAAGTTGAAGAAAAATGAAGAAGAAGAAGAAACAGAAGAGCATGAAATGGATATGAAATTGGTTGGAAGAGTACTAAAAATGTCAAGAATAACAACAGATCAATTGATTTGGTGTAGGAACAAATTGAGTAGAATTCATTTCATAAACACAAAAATACATTTAGAACCTTCTTTCTTTCTTTTTCCCTGTTGA

Protein sequence

MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFSFRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQSELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITNDMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKEKMRSGHCILKKLKKNEEEEETEEHEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHFINTKIHLEPSFFLFPC
Homology
BLAST of Carg22896 vs. NCBI nr
Match: KAG7022223.1 (hypothetical protein SDJN02_15953, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 618/618 (100.00%), Postives = 618/618 (100.00%), Query Frame = 0

Query: 1   MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFS 60
           MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFS
Sbjct: 1   MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFS 60

Query: 61  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 120
           FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY
Sbjct: 61  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 120

Query: 121 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 180
           EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS
Sbjct: 121 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 180

Query: 181 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 240
           ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI
Sbjct: 181 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 240

Query: 241 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 300
           EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM
Sbjct: 241 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 300

Query: 301 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 360
           RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE
Sbjct: 301 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 360

Query: 361 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 420
           VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE
Sbjct: 361 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 420

Query: 421 NEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITN 480
           NEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITN
Sbjct: 421 NEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITN 480

Query: 481 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 540
           DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE
Sbjct: 481 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 540

Query: 541 RKVKEKMRSGHCILKKLKKNEEEEETEEHEMDMKLVGRVLKMSRITTDQLIWCRNKLSRI 600
           RKVKEKMRSGHCILKKLKKNEEEEETEEHEMDMKLVGRVLKMSRITTDQLIWCRNKLSRI
Sbjct: 541 RKVKEKMRSGHCILKKLKKNEEEEETEEHEMDMKLVGRVLKMSRITTDQLIWCRNKLSRI 600

Query: 601 HFINTKIHLEPSFFLFPC 619
           HFINTKIHLEPSFFLFPC
Sbjct: 601 HFINTKIHLEPSFFLFPC 618

BLAST of Carg22896 vs. NCBI nr
Match: XP_022930474.1 (uncharacterized protein LOC111436904 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 603/623 (96.79%), Postives = 604/623 (96.95%), Query Frame = 0

Query: 1   MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFS 60
           MSDEERGRPLD GRNMEISDVVLVDDGGGCDDLQPPEK TSGGGAPEVSDEKGTNEFVFS
Sbjct: 2   MSDEERGRPLDGGRNMEISDVVLVDDGGGCDDLQPPEKETSGGGAPEVSDEKGTNEFVFS 61

Query: 61  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 120
           FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY
Sbjct: 62  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 121

Query: 121 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 180
           EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS
Sbjct: 122 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 181

Query: 181 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 240
           ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI
Sbjct: 182 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 241

Query: 241 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 300
           EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM
Sbjct: 242 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 301

Query: 301 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 360
           RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE
Sbjct: 302 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 361

Query: 361 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 420
           VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE
Sbjct: 362 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 421

Query: 421 NEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITN 480
           NEAFEGPRVENYVKQRCVVRNLLQVPVIR        EDKTRDRRKARQWRDHENEAITN
Sbjct: 422 NEAFEGPRVENYVKQRCVVRNLLQVPVIR--------EDKTRDRRKARQWRDHENEAITN 481

Query: 481 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 540
           DMLVEILQESIRVISQFIRADKVHN TATLKRPKKFQVELQDP DMQLLT+IQVDLQKKE
Sbjct: 482 DMLVEILQESIRVISQFIRADKVHNLTATLKRPKKFQVELQDPADMQLLTDIQVDLQKKE 541

Query: 541 RKVKEKMRSGHCILKKLKKNEEEEETEE-----HEMDMKLVGRVLKMSRITTDQLIWCRN 600
           RKVKEKMRSGHCILKKLKKNEEEEETE       EMDMKLVGRVLKMSRITTDQLIWCRN
Sbjct: 542 RKVKEKMRSGHCILKKLKKNEEEEETEGAVSFFSEMDMKLVGRVLKMSRITTDQLIWCRN 601

Query: 601 KLSRIHFINTKIHLEPSFFLFPC 619
           KLSRIHFINTKIHLEPSFFLFPC
Sbjct: 602 KLSRIHFINTKIHLEPSFFLFPC 616

BLAST of Carg22896 vs. NCBI nr
Match: XP_023529275.1 (uncharacterized protein LOC111792173 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 597/623 (95.83%), Postives = 601/623 (96.47%), Query Frame = 0

Query: 1   MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFS 60
           MSDEERGRPLD GRNMEISDVVLVDDGGGCDDLQPPE+ TSGGGAPEVSDEKGTNEFVFS
Sbjct: 2   MSDEERGRPLDGGRNMEISDVVLVDDGGGCDDLQPPEEETSGGGAPEVSDEKGTNEFVFS 61

Query: 61  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 120
           FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY
Sbjct: 62  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 121

Query: 121 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 180
           EIPRTGDFS+REFSGKVLDSQ ITVSYE+SGEIPESEVIGETAELSST SREEQMAEKQS
Sbjct: 122 EIPRTGDFSIREFSGKVLDSQTITVSYEISGEIPESEVIGETAELSSTNSREEQMAEKQS 181

Query: 181 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 240
           ELKQRDER TDFVSDYDMNLKMGGYE DEETNEELEKWGEEEEEEELNGLETEWEHQELI
Sbjct: 182 ELKQRDERETDFVSDYDMNLKMGGYEADEETNEELEKWGEEEEEEELNGLETEWEHQELI 241

Query: 241 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 300
           EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM
Sbjct: 242 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 301

Query: 301 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 360
           RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE
Sbjct: 302 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 361

Query: 361 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 420
           VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE
Sbjct: 362 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 421

Query: 421 NEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITN 480
           NEAFEGPRVENYVKQRCVVRNLLQVPVIR        EDKTRDRRKARQ RDHENEAITN
Sbjct: 422 NEAFEGPRVENYVKQRCVVRNLLQVPVIR--------EDKTRDRRKARQLRDHENEAITN 481

Query: 481 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 540
           DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE
Sbjct: 482 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 541

Query: 541 RKVKEKMRSGHCILKKLKKNEEEEETEE-----HEMDMKLVGRVLKMSRITTDQLIWCRN 600
           RKVKEKMRSGHCILKKLKKNEE+EETE       EMDMKLVGRVLKMSRITTDQLIWCRN
Sbjct: 542 RKVKEKMRSGHCILKKLKKNEEDEETEGAVSFFSEMDMKLVGRVLKMSRITTDQLIWCRN 601

Query: 601 KLSRIHFINTKIHLEPSFFLFPC 619
           KLSRIHFINTKIHLEPSFFLFPC
Sbjct: 602 KLSRIHFINTKIHLEPSFFLFPC 616

BLAST of Carg22896 vs. NCBI nr
Match: XP_022930467.1 (uncharacterized protein LOC111436904 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 596/616 (96.75%), Postives = 597/616 (96.92%), Query Frame = 0

Query: 8   RPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFSFRFQTYE 67
           RPLD GRNMEISDVVLVDDGGGCDDLQPPEK TSGGGAPEVSDEKGTNEFVFSFRFQTYE
Sbjct: 46  RPLDGGRNMEISDVVLVDDGGGCDDLQPPEKETSGGGAPEVSDEKGTNEFVFSFRFQTYE 105

Query: 68  EFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGD 127
           EFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGD
Sbjct: 106 EFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGD 165

Query: 128 FSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQSELKQRDE 187
           FSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQSELKQRDE
Sbjct: 166 FSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQSELKQRDE 225

Query: 188 RGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMEL 247
           RGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMEL
Sbjct: 226 RGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMEL 285

Query: 248 KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN 307
           KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN
Sbjct: 286 KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN 345

Query: 308 YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM 367
           YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM
Sbjct: 346 YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM 405

Query: 368 VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP 427
           VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP
Sbjct: 406 VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP 465

Query: 428 RVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITNDMLVEIL 487
           RVENYVKQRCVVRNLLQVPVIR        EDKTRDRRKARQWRDHENEAITNDMLVEIL
Sbjct: 466 RVENYVKQRCVVRNLLQVPVIR--------EDKTRDRRKARQWRDHENEAITNDMLVEIL 525

Query: 488 QESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKEKM 547
           QESIRVISQFIRADKVHN TATLKRPKKFQVELQDP DMQLLT+IQVDLQKKERKVKEKM
Sbjct: 526 QESIRVISQFIRADKVHNLTATLKRPKKFQVELQDPADMQLLTDIQVDLQKKERKVKEKM 585

Query: 548 RSGHCILKKLKKNEEEEETEE-----HEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF 607
           RSGHCILKKLKKNEEEEETE       EMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF
Sbjct: 586 RSGHCILKKLKKNEEEEETEGAVSFFSEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF 645

Query: 608 INTKIHLEPSFFLFPC 619
           INTKIHLEPSFFLFPC
Sbjct: 646 INTKIHLEPSFFLFPC 653

BLAST of Carg22896 vs. NCBI nr
Match: XP_022970620.1 (uncharacterized protein LOC111469544 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 591/623 (94.86%), Postives = 597/623 (95.83%), Query Frame = 0

Query: 1   MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFS 60
           MSDEERGRPLD GRNMEISDVVLVDDGGGCDDLQPPE+ TSGGGAPEVSDEKGTNEFVFS
Sbjct: 2   MSDEERGRPLDGGRNMEISDVVLVDDGGGCDDLQPPEEETSGGGAPEVSDEKGTNEFVFS 61

Query: 61  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 120
           FRFQTYEEFTKSNK+NLGCERLDSLSNRYEF PEKSTSHFVEEP IPSFTVEVLNSCSN+
Sbjct: 62  FRFQTYEEFTKSNKRNLGCERLDSLSNRYEFFPEKSTSHFVEEPGIPSFTVEVLNSCSNF 121

Query: 121 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 180
           EIPRTGDFSVREFSGKVLDSQ ITVSYEVSGEIPESEVIGETAELSST SREEQMAEKQS
Sbjct: 122 EIPRTGDFSVREFSGKVLDSQTITVSYEVSGEIPESEVIGETAELSSTNSREEQMAEKQS 181

Query: 181 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 240
           ELKQRDER TDFVSDYD+NL +GGYEPDEETNEELEKWG  EEEEELNGLETEWEHQELI
Sbjct: 182 ELKQRDERETDFVSDYDINLNVGGYEPDEETNEELEKWG--EEEEELNGLETEWEHQELI 241

Query: 241 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 300
           EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM
Sbjct: 242 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 301

Query: 301 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 360
           RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE
Sbjct: 302 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 361

Query: 361 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 420
           VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE
Sbjct: 362 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 421

Query: 421 NEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITN 480
           NEAFEGPRVENYVKQRCVVRNLLQVPVIR        EDKTRDRRKARQWRDHENEAITN
Sbjct: 422 NEAFEGPRVENYVKQRCVVRNLLQVPVIR--------EDKTRDRRKARQWRDHENEAITN 481

Query: 481 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 540
           DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE
Sbjct: 482 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 541

Query: 541 RKVKEKMRSGHCILKKLKKNEEEEETEE-----HEMDMKLVGRVLKMSRITTDQLIWCRN 600
           RKVKEKMRSGHCILKKLKKNEEEEETE       EMDMKLVGRVLKMSRITTDQLIWCRN
Sbjct: 542 RKVKEKMRSGHCILKKLKKNEEEEETEGAVSFFSEMDMKLVGRVLKMSRITTDQLIWCRN 601

Query: 601 KLSRIHFINTKIHLEPSFFLFPC 619
           KLSRIHF NT+IHLEPSFFLFPC
Sbjct: 602 KLSRIHFKNTRIHLEPSFFLFPC 614

BLAST of Carg22896 vs. ExPASy TrEMBL
Match: A0A6J1ERH8 (uncharacterized protein LOC111436904 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436904 PE=4 SV=1)

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 603/623 (96.79%), Postives = 604/623 (96.95%), Query Frame = 0

Query: 1   MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFS 60
           MSDEERGRPLD GRNMEISDVVLVDDGGGCDDLQPPEK TSGGGAPEVSDEKGTNEFVFS
Sbjct: 2   MSDEERGRPLDGGRNMEISDVVLVDDGGGCDDLQPPEKETSGGGAPEVSDEKGTNEFVFS 61

Query: 61  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 120
           FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY
Sbjct: 62  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 121

Query: 121 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 180
           EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS
Sbjct: 122 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 181

Query: 181 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 240
           ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI
Sbjct: 182 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 241

Query: 241 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 300
           EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM
Sbjct: 242 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 301

Query: 301 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 360
           RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE
Sbjct: 302 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 361

Query: 361 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 420
           VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE
Sbjct: 362 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 421

Query: 421 NEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITN 480
           NEAFEGPRVENYVKQRCVVRNLLQVPVIR        EDKTRDRRKARQWRDHENEAITN
Sbjct: 422 NEAFEGPRVENYVKQRCVVRNLLQVPVIR--------EDKTRDRRKARQWRDHENEAITN 481

Query: 481 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 540
           DMLVEILQESIRVISQFIRADKVHN TATLKRPKKFQVELQDP DMQLLT+IQVDLQKKE
Sbjct: 482 DMLVEILQESIRVISQFIRADKVHNLTATLKRPKKFQVELQDPADMQLLTDIQVDLQKKE 541

Query: 541 RKVKEKMRSGHCILKKLKKNEEEEETEE-----HEMDMKLVGRVLKMSRITTDQLIWCRN 600
           RKVKEKMRSGHCILKKLKKNEEEEETE       EMDMKLVGRVLKMSRITTDQLIWCRN
Sbjct: 542 RKVKEKMRSGHCILKKLKKNEEEEETEGAVSFFSEMDMKLVGRVLKMSRITTDQLIWCRN 601

Query: 601 KLSRIHFINTKIHLEPSFFLFPC 619
           KLSRIHFINTKIHLEPSFFLFPC
Sbjct: 602 KLSRIHFINTKIHLEPSFFLFPC 616

BLAST of Carg22896 vs. ExPASy TrEMBL
Match: A0A6J1EQK9 (uncharacterized protein LOC111436904 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436904 PE=4 SV=1)

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 596/616 (96.75%), Postives = 597/616 (96.92%), Query Frame = 0

Query: 8   RPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFSFRFQTYE 67
           RPLD GRNMEISDVVLVDDGGGCDDLQPPEK TSGGGAPEVSDEKGTNEFVFSFRFQTYE
Sbjct: 46  RPLDGGRNMEISDVVLVDDGGGCDDLQPPEKETSGGGAPEVSDEKGTNEFVFSFRFQTYE 105

Query: 68  EFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGD 127
           EFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGD
Sbjct: 106 EFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGD 165

Query: 128 FSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQSELKQRDE 187
           FSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQSELKQRDE
Sbjct: 166 FSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQSELKQRDE 225

Query: 188 RGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMEL 247
           RGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMEL
Sbjct: 226 RGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMEL 285

Query: 248 KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN 307
           KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN
Sbjct: 286 KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN 345

Query: 308 YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM 367
           YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM
Sbjct: 346 YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM 405

Query: 368 VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP 427
           VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP
Sbjct: 406 VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP 465

Query: 428 RVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITNDMLVEIL 487
           RVENYVKQRCVVRNLLQVPVIR        EDKTRDRRKARQWRDHENEAITNDMLVEIL
Sbjct: 466 RVENYVKQRCVVRNLLQVPVIR--------EDKTRDRRKARQWRDHENEAITNDMLVEIL 525

Query: 488 QESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKEKM 547
           QESIRVISQFIRADKVHN TATLKRPKKFQVELQDP DMQLLT+IQVDLQKKERKVKEKM
Sbjct: 526 QESIRVISQFIRADKVHNLTATLKRPKKFQVELQDPADMQLLTDIQVDLQKKERKVKEKM 585

Query: 548 RSGHCILKKLKKNEEEEETEE-----HEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF 607
           RSGHCILKKLKKNEEEEETE       EMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF
Sbjct: 586 RSGHCILKKLKKNEEEEETEGAVSFFSEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF 645

Query: 608 INTKIHLEPSFFLFPC 619
           INTKIHLEPSFFLFPC
Sbjct: 646 INTKIHLEPSFFLFPC 653

BLAST of Carg22896 vs. ExPASy TrEMBL
Match: A0A6J1I3C6 (uncharacterized protein LOC111469544 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469544 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 591/623 (94.86%), Postives = 597/623 (95.83%), Query Frame = 0

Query: 1   MSDEERGRPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFS 60
           MSDEERGRPLD GRNMEISDVVLVDDGGGCDDLQPPE+ TSGGGAPEVSDEKGTNEFVFS
Sbjct: 2   MSDEERGRPLDGGRNMEISDVVLVDDGGGCDDLQPPEEETSGGGAPEVSDEKGTNEFVFS 61

Query: 61  FRFQTYEEFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNY 120
           FRFQTYEEFTKSNK+NLGCERLDSLSNRYEF PEKSTSHFVEEP IPSFTVEVLNSCSN+
Sbjct: 62  FRFQTYEEFTKSNKRNLGCERLDSLSNRYEFFPEKSTSHFVEEPGIPSFTVEVLNSCSNF 121

Query: 121 EIPRTGDFSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQS 180
           EIPRTGDFSVREFSGKVLDSQ ITVSYEVSGEIPESEVIGETAELSST SREEQMAEKQS
Sbjct: 122 EIPRTGDFSVREFSGKVLDSQTITVSYEVSGEIPESEVIGETAELSSTNSREEQMAEKQS 181

Query: 181 ELKQRDERGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELI 240
           ELKQRDER TDFVSDYD+NL +GGYEPDEETNEELEKWG  EEEEELNGLETEWEHQELI
Sbjct: 182 ELKQRDERETDFVSDYDINLNVGGYEPDEETNEELEKWG--EEEEELNGLETEWEHQELI 241

Query: 241 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 300
           EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM
Sbjct: 242 EQLKMELKKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERM 301

Query: 301 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 360
           RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE
Sbjct: 302 RKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIRE 361

Query: 361 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 420
           VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE
Sbjct: 362 VHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLE 421

Query: 421 NEAFEGPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITN 480
           NEAFEGPRVENYVKQRCVVRNLLQVPVIR        EDKTRDRRKARQWRDHENEAITN
Sbjct: 422 NEAFEGPRVENYVKQRCVVRNLLQVPVIR--------EDKTRDRRKARQWRDHENEAITN 481

Query: 481 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 540
           DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE
Sbjct: 482 DMLVEILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKE 541

Query: 541 RKVKEKMRSGHCILKKLKKNEEEEETEE-----HEMDMKLVGRVLKMSRITTDQLIWCRN 600
           RKVKEKMRSGHCILKKLKKNEEEEETE       EMDMKLVGRVLKMSRITTDQLIWCRN
Sbjct: 542 RKVKEKMRSGHCILKKLKKNEEEEETEGAVSFFSEMDMKLVGRVLKMSRITTDQLIWCRN 601

Query: 601 KLSRIHFINTKIHLEPSFFLFPC 619
           KLSRIHF NT+IHLEPSFFLFPC
Sbjct: 602 KLSRIHFKNTRIHLEPSFFLFPC 614

BLAST of Carg22896 vs. ExPASy TrEMBL
Match: A0A6J1HZL3 (uncharacterized protein LOC111469544 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469544 PE=4 SV=1)

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 584/616 (94.81%), Postives = 590/616 (95.78%), Query Frame = 0

Query: 8   RPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFSFRFQTYE 67
           RPLD GRNMEISDVVLVDDGGGCDDLQPPE+ TSGGGAPEVSDEKGTNEFVFSFRFQTYE
Sbjct: 46  RPLDGGRNMEISDVVLVDDGGGCDDLQPPEEETSGGGAPEVSDEKGTNEFVFSFRFQTYE 105

Query: 68  EFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGD 127
           EFTKSNK+NLGCERLDSLSNRYEF PEKSTSHFVEEP IPSFTVEVLNSCSN+EIPRTGD
Sbjct: 106 EFTKSNKRNLGCERLDSLSNRYEFFPEKSTSHFVEEPGIPSFTVEVLNSCSNFEIPRTGD 165

Query: 128 FSVREFSGKVLDSQRITVSYEVSGEIPESEVIGETAELSSTKSREEQMAEKQSELKQRDE 187
           FSVREFSGKVLDSQ ITVSYEVSGEIPESEVIGETAELSST SREEQMAEKQSELKQRDE
Sbjct: 166 FSVREFSGKVLDSQTITVSYEVSGEIPESEVIGETAELSSTNSREEQMAEKQSELKQRDE 225

Query: 188 RGTDFVSDYDMNLKMGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMEL 247
           R TDFVSDYD+NL +GGYEPDEETNEELEKWG  EEEEELNGLETEWEHQELIEQLKMEL
Sbjct: 226 RETDFVSDYDINLNVGGYEPDEETNEELEKWG--EEEEELNGLETEWEHQELIEQLKMEL 285

Query: 248 KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN 307
           KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN
Sbjct: 286 KKVRASGLPTISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILN 345

Query: 308 YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM 367
           YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM
Sbjct: 346 YQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLEM 405

Query: 368 VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP 427
           VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP
Sbjct: 406 VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP 465

Query: 428 RVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITNDMLVEIL 487
           RVENYVKQRCVVRNLLQVPVIR        EDKTRDRRKARQWRDHENEAITNDMLVEIL
Sbjct: 466 RVENYVKQRCVVRNLLQVPVIR--------EDKTRDRRKARQWRDHENEAITNDMLVEIL 525

Query: 488 QESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKEKM 547
           QESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKEKM
Sbjct: 526 QESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKEKM 585

Query: 548 RSGHCILKKLKKNEEEEETEE-----HEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF 607
           RSGHCILKKLKKNEEEEETE       EMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF
Sbjct: 586 RSGHCILKKLKKNEEEEETEGAVSFFSEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHF 645

Query: 608 INTKIHLEPSFFLFPC 619
            NT+IHLEPSFFLFPC
Sbjct: 646 KNTRIHLEPSFFLFPC 651

BLAST of Carg22896 vs. ExPASy TrEMBL
Match: A0A6J1BVT2 (uncharacterized protein LOC111005168 OS=Momordica charantia OX=3673 GN=LOC111005168 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 7.3e-214
Identity = 430/671 (64.08%), Postives = 498/671 (74.22%), Query Frame = 0

Query: 8   RPLDDGRNMEISDVVLVDDGGGCDDLQPPEKGTSGGGAPEVSDEKGTNEFVFSFRFQTYE 67
           R LD G+ +   D+ + + G      +  E+    GG P +SDE+ +N+FVFSF+F++YE
Sbjct: 41  RALDGGKKL---DMEMENSGDSRLPEEEEEEEEPAGGRPPISDEEESNDFVFSFKFRSYE 100

Query: 68  EFTKSNKKNLGCERLDSLSNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGD 127
           EF+K+N+++ G E  +SLSNRYEFLPEKSTS FV    IPSF VEVLNSCS+ +IP TGD
Sbjct: 101 EFSKTNREDFGSEGFNSLSNRYEFLPEKSTSFFV----IPSFKVEVLNSCSSNQIPGTGD 160

Query: 128 FSVREFSGKVLDSQRITVSYE----------VSGEIPESEVIGETA-------------- 187
           F V EF GK+ +S+ I                SG++ E E+   +A              
Sbjct: 161 FPVEEFPGKIPESESIGEEITESSVYGGEEGFSGKVSEEEITQSSANGNEEFPGKVSEEE 220

Query: 188 --------------ELSSTKSREEQMAEKQSELKQR----------DERGTDFVSDYDMN 247
                         ++S +K  E+++ EKQ +++QR          D  G+D  SD   +
Sbjct: 221 ITECSANGKEEFSGKISESKPVEDEITEKQRKVEQRVEEEEEEEEEDFTGSDSDSDSGFD 280

Query: 248 LKMGGYEPDEETNEELEKWG---EEEEEEELNGLETEWEHQELIEQLKMELKKVRASGLP 307
             +GGYEPDEE NEE E+ G     EE +ELNGLETEWEHQELIEQLKMELKKVRA GLP
Sbjct: 281 FHVGGYEPDEEINEEAERGGGGERTEESDELNGLETEWEHQELIEQLKMELKKVRARGLP 340

Query: 308 TISEESESPKIMEELKPWKIDERFKRGDLMEELHSFYRSYRERMRKLDILNYQKMYAMGV 367
           TI EESESPKIMEELKPWKID++F+ GDLMEELH FYR+YRERMRKLDILNYQKMYAMGV
Sbjct: 341 TIFEESESPKIMEELKPWKIDDKFQHGDLMEELHKFYRTYRERMRKLDILNYQKMYAMGV 400

Query: 368 LQSKDPLKSFSSNTKSSSPSITSV----LRLYRQKKCQVDPMKDFIREVHCDLEMVYVAQ 427
           LQSKDPLKSF SN+KSSSPSITS+    LRLYRQKK QVDPMK+FIREVHCDLEMVYV Q
Sbjct: 401 LQSKDPLKSFCSNSKSSSPSITSLLSQNLRLYRQKKSQVDPMKNFIREVHCDLEMVYVGQ 460

Query: 428 MCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGPRVENY 487
           MCLSWEFIQWQY KALDLWESEPHGLHHYNEVAGEFQ FQVLL+RFLENEAFEGPRVENY
Sbjct: 461 MCLSWEFIQWQYEKALDLWESEPHGLHHYNEVAGEFQQFQVLLQRFLENEAFEGPRVENY 520

Query: 488 VKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITNDMLVEILQESIR 547
           VK RCV R+LLQVPVIR        EDK RDRRKAR+  DHENEAIT+DMLVEILQESIR
Sbjct: 521 VKHRCVARHLLQVPVIR--------EDKMRDRRKARRVIDHENEAITSDMLVEILQESIR 580

Query: 548 VISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKEKMRSGHC 607
           +I QFIR+DK     ATLKR KKFQVELQDP D QLL EIQ DL KKERK+KE +RSGHC
Sbjct: 581 IIWQFIRSDK---DQATLKRSKKFQVELQDPVDEQLLMEIQADLHKKERKLKEIVRSGHC 640

Query: 608 ILKKLKKNEEEEETEE----HEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHFIN-TKI 619
           ILK+L++NEEEE   +     E+DMKLVGRVL+MSRITTDQL+WCRNKLSRI+F+N  KI
Sbjct: 641 ILKRLQRNEEEETEGDLCFFCEVDMKLVGRVLRMSRITTDQLVWCRNKLSRINFLNRRKI 693

BLAST of Carg22896 vs. TAIR 10
Match: AT5G39785.1 (Protein of unknown function (DUF1666) )

HSP 1 Score: 397.9 bits (1021), Expect = 1.5e-110
Identity = 257/623 (41.25%), Postives = 380/623 (61.00%), Query Frame = 0

Query: 48  VSDEKGTNE-----FVFSFRFQTYEEFTKSNKKNL----GCERLDSL------------- 107
           + DE+  +E     F F+F+ +T+E  ++   ++L    G E   S              
Sbjct: 9   IEDEEEVSESPKLVFKFAFQTETFERLSREGYRSLSDDCGVESESSFLSSDSSPVIFTAA 68

Query: 108 SNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGDFSVREFSGKVLDSQRITV 167
           + +YE    K+ +  +E P+  +F VE    C   +  +T D     F G   + +  T 
Sbjct: 69  ARKYETPSAKNLTFVLENPKAATFRVE---DCLKEKQEKTEDLGYSVFHG---EDKVKTE 128

Query: 168 SYEVSG-EIPESEVIGETAELSSTKSREEQMAEKQSELKQRDERGTDFVSDYDM---NLK 227
            Y VS  +  +   + E   L S    +    +        DE G  F+SD D    +LK
Sbjct: 129 DYSVSSFKKKKIRFLTEEDFLES----DSDFVDSSQTFTSNDEDG--FLSDSDFAETSLK 188

Query: 228 MGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMELKKVRA-SGLPTISE 287
            G     + +    +   EEEEEE+ NG E+ WEHQ+LIEQLKME+KKV+A  GL TI E
Sbjct: 189 KGQNRKSDNSGSGSD--SEEEEEEDTNGFESLWEHQDLIEQLKMEMKKVKAIGGLTTILE 248

Query: 288 ESES----PKIMEELKPWKIDE--RFKRGDLMEELHSFYRSYRERMRKLDILNYQKMYAM 347
           E E     PKIME+LKPW+I+E  +FK  D + E+H F+RSYRERMRKLDIL++QK YA+
Sbjct: 249 EEEEDDDCPKIMEDLKPWRIEEEKKFKHVDTIGEVHKFHRSYRERMRKLDILSFQKSYAL 308

Query: 348 GVLQSKDPLKSFSSNTKSSSPSITSV-------LRLYRQKKCQVDPMKDFIREVHCDLEM 407
           G+LQSK P +  +++T  S+PS TS        +RL++ KK +++PM  F++E+  +LE 
Sbjct: 309 GLLQSKSPQQ--ATSTLGSNPSQTSFSSVFSVNIRLWKAKKSEIEPMVQFVKEIQGELEN 368

Query: 408 VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP 467
           VYV QMCLSWE + WQY KA++L ES+ +G   YNEVAGEFQ FQVLL+RFLENE FE P
Sbjct: 369 VYVGQMCLSWEILHWQYEKAIELLESDVYGSRRYNEVAGEFQQFQVLLQRFLENEPFEEP 428

Query: 468 RVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENE---AITNDMLV 527
           RV++Y+K+RCV+RNLLQ+PVIR        ED  +D++  R+ RD+E      I +D LV
Sbjct: 429 RVQHYIKRRCVLRNLLQIPVIR--------EDGNKDKKNGRR-RDYEENNDGVIKSDQLV 488

Query: 528 EILQESIRVISQFIRADKVHNPTATLKRPKKFQVE---LQDPTDMQLLTEIQVDLQKKER 587
           EI++E+IR+  +F+R DK+ +     K   K Q+E    +D  D+++  E++  LQ KE+
Sbjct: 489 EIMEETIRLFWRFVRCDKLTSSIHDQKSRTKSQIEPDHEEDSEDLEMFAEVKSQLQNKEK 548

Query: 588 KVKEKMRSGHCILKKLKKNEEEEETEEH------EMDMKLVGRVLKMSRITTDQLIWCRN 619
           ++++ ++S  CI+++ +K++EE+ TE+       ++DMKLV RVL MS++T D L+WC N
Sbjct: 549 RLRDVLKSERCIIRRFQKHKEEDSTEDQVLHFFSQVDMKLVTRVLNMSKLTRDHLVWCHN 606

BLAST of Carg22896 vs. TAIR 10
Match: AT5G39785.2 (Protein of unknown function (DUF1666) )

HSP 1 Score: 391.3 bits (1004), Expect = 1.4e-108
Identity = 256/624 (41.03%), Postives = 379/624 (60.74%), Query Frame = 0

Query: 48  VSDEKGTNE-----FVFSFRFQTYEEFTKSNKKNL----GCERLDSL------------- 107
           + DE+  +E     F F+F+ +T+E  ++   ++L    G E   S              
Sbjct: 9   IEDEEEVSESPKLVFKFAFQTETFERLSREGYRSLSDDCGVESESSFLSSDSSPVIFTAA 68

Query: 108 SNRYEFLPEKSTSHFVEEPEIPSFTVEVLNSCSNYEIPRTGDFSVREFSGKVLDSQRITV 167
           + +YE    K+ +  +E P+  +F VE    C   +  +T D     F G   + +  T 
Sbjct: 69  ARKYETPSAKNLTFVLENPKAATFRVE---DCLKEKQEKTEDLGYSVFHG---EDKVKTE 128

Query: 168 SYEVSG-EIPESEVIGETAELSSTKSREEQMAEKQSELKQRDERGTDFVSDYDM---NLK 227
            Y VS  +  +   + E   L S    +    +        DE G  F+SD D    +LK
Sbjct: 129 DYSVSSFKKKKIRFLTEEDFLES----DSDFVDSSQTFTSNDEDG--FLSDSDFAETSLK 188

Query: 228 MGGYEPDEETNEELEKWGEEEEEEELNGLETEWEHQELIEQLKMELKKVRA-SGLPTISE 287
            G     + +    +   EEEEEE+ NG E+ WEHQ+LIEQLKME+KKV+A  GL TI E
Sbjct: 189 KGQNRKSDNSGSGSD--SEEEEEEDTNGFESLWEHQDLIEQLKMEMKKVKAIGGLTTILE 248

Query: 288 ESES----PKIMEELKPWKIDE--RFKRGDLMEELHSFYRSYRERMRKLDILNYQKMYAM 347
           E E     PKIME+LKPW+I+E  +FK  D + E+H F+RSYRERMRKLDIL++QK YA+
Sbjct: 249 EEEEDDDCPKIMEDLKPWRIEEEKKFKHVDTIGEVHKFHRSYRERMRKLDILSFQKSYAL 308

Query: 348 GVLQSKDPLKSFSSNTKSSSPSITSV-------LRLYRQKKCQVDPMKDFIREVHCDLEM 407
           G+LQSK P +  +++T  S+PS TS        +RL++ KK +++PM  F++E+  +LE 
Sbjct: 309 GLLQSKSPQQ--ATSTLGSNPSQTSFSSVFSVNIRLWKAKKSEIEPMVQFVKEIQGELEN 368

Query: 408 VYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFEGP 467
           VYV QMCLSWE + WQY KA++L ES+ +G   YNEVAGEFQ FQVLL+RFLENE FE P
Sbjct: 369 VYVGQMCLSWEILHWQYEKAIELLESDVYGSRRYNEVAGEFQQFQVLLQRFLENEPFEEP 428

Query: 468 RVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENE---AITNDMLV 527
           RV++Y+K+RCV+RNLLQ+PVIR        ED  +D++  R+ RD+E      I +D LV
Sbjct: 429 RVQHYIKRRCVLRNLLQIPVIR--------EDGNKDKKNGRR-RDYEENNDGVIKSDQLV 488

Query: 528 EILQESIRVISQFIRADKVHNPTATLKRPKKFQVE---LQDPTDMQLLTEIQVDLQK-KE 587
           EI++E+IR+  +F+R DK+ +     K   K Q+E    +D  D+++  E++  LQ   E
Sbjct: 489 EIMEETIRLFWRFVRCDKLTSSIHDQKSRTKSQIEPDHEEDSEDLEMFAEVKSQLQNVSE 548

Query: 588 RKVKEKMRSGHCILKKLKKNEEEEETEEH------EMDMKLVGRVLKMSRITTDQLIWCR 619
           +++++ ++S  CI+++ +K++EE+ TE+       ++DMKLV RVL MS++T D L+WC 
Sbjct: 549 KRLRDVLKSERCIIRRFQKHKEEDSTEDQVLHFFSQVDMKLVTRVLNMSKLTRDHLVWCH 607

BLAST of Carg22896 vs. TAIR 10
Match: AT1G69610.1 (Protein of unknown function (DUF1666) )

HSP 1 Score: 261.9 bits (668), Expect = 1.3e-69
Identity = 200/559 (35.78%), Postives = 305/559 (54.56%), Query Frame = 0

Query: 82  LDSLSNRYEFLPE-KSTSHFVEEPEIPSFTVEVLNSCS--NYEIPRTGDFSVREFSGKVL 141
           L S+S R EF  + KS   FVEEP+  SF     ++ +    E  +   FS  + S  VL
Sbjct: 97  LVSISKRCEFYSDNKSIVGFVEEPKAFSFKFHEYSAITVQEEEKKKMVSFSDEKTSSMVL 156

Query: 142 DSQRITVSYEVSGEIPESEVIGETAELSSTKSR--EEQMAEKQSELKQRDERGTDFVSDY 201
           +         +   + E  ++ E       K     E +   +  L   D+   +     
Sbjct: 157 EKSE--SDGFMKEHVKEEMLVYEFMSCGVLKESLVHENLVGGEDNLFDDDDGFIELNPSL 216

Query: 202 DMNLKMGGYEPDEE--TNEELEKWGEEEEEEELNGL-------ETEWEHQELIEQLKMEL 261
            ++    G E +EE    EE  K G +E+EE  +         + E+EH ++IE+LK EL
Sbjct: 217 QISNFAYGEEEEEEFIHREEEMKMGFDEQEEVYDEFDDGSDSDDDEFEHSDVIEKLKTEL 276

Query: 262 KKVRASGLPTISEESESPKIMEELKPWKIDER-FKRGDLMEELHSFYRSYRERMRKLDIL 321
           +  R  GL TI EESE+P  ++ELKP KI+ +  +  D + E+H  Y++Y  +MRKLD++
Sbjct: 277 RTARTGGLCTILEESETP--LQELKPLKIEPKPDQHKDRIAEIHKVYKNYAVKMRKLDVI 336

Query: 322 NYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRLYRQKKCQVDPMKDFIREVHCDLE 381
           + Q M+++ +L+ KD  K   +  K    S+   +  +++   + DP +  ++E   D E
Sbjct: 337 DSQTMHSISLLKLKDSSKPSRNTDKPPKSSLHQNIWPFKKHTLECDPSERLVKEASRDFE 396

Query: 382 MVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFE- 441
            VYV Q+CLSWE ++WQY K L+         + YN VAGEFQ FQVLL+RF+ENE F+ 
Sbjct: 397 TVYVGQVCLSWEMLRWQYDKVLEF--DSQVTTYQYNLVAGEFQLFQVLLQRFVENEPFQN 456

Query: 442 GPRVENYVKQRCVVRNLLQVPVIRANLVPFLIEDKTRDRRKARQWRDHENEAITNDMLVE 501
             RVE Y+K R   +N LQ+P++R             DR   ++ R     A+  +ML E
Sbjct: 457 SSRVETYLKNRRHFQNFLQIPLVR------------DDRSSKKKCRYEGEFAVKTEMLRE 516

Query: 502 ILQESIRVISQFIRADKVHNPTATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKE 561
           I++ES+ V  +F+ ADK    T+ +K   + QV  QD  D++LLT+I+  LQKKE+K+KE
Sbjct: 517 IIRESMSVFWEFLCADK-DEFTSMMKVSHQTQVSPQDSLDLELLTDIRTHLQKKEKKLKE 576

Query: 562 KMRSGHCILKKLKKNEE------EEETEEHEMDMKLVGRVLKMSRITTDQLIWCRNKLSR 619
             RS  CI+KKLKKNE       ++E    +++++LV RV+ MS++TT++L WC+ KL +
Sbjct: 577 IQRSQSCIVKKLKKNESKSSIGVKDELLIAKIELRLVSRVMYMSKLTTEKLHWCQEKLEK 636

BLAST of Carg22896 vs. TAIR 10
Match: AT3G20260.1 (Protein of unknown function (DUF1666) )

HSP 1 Score: 115.2 bits (287), Expect = 1.9e-25
Identity = 119/412 (28.88%), Postives = 192/412 (46.60%), Query Frame = 0

Query: 229 GLETEWEHQELI-EQLKMELKKVRASG----LPTISEESESPKIMEELKPWKIDE-RFKR 288
           G E E +  + I  ++K  LK++R +     +P   EE E    ++E      D+   + 
Sbjct: 47  GDEIEDDDDDFITNEVKRRLKELRRNSFMVLIPEEEEEEEEESYLDEDDDDGEDKCSSEW 106

Query: 289 GDLMEE-------LHSFYRSYRERMRKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSP 348
            D++ E         + Y  Y ERM   D L+ Q++   G+  +  P    + + +S+S 
Sbjct: 107 RDVVAEGLQWWGGFDAVYEKYCERMLFFDRLSSQQLKETGIGIAPSP---STPSPRSASK 166

Query: 349 SITSVLRLYRQKKCQVDPMKDF-------IREVHCDLEMVYVAQMCLSWEFIQWQYGKAL 408
            ++S  R    KK  V P +D        + + + DLE  YVAQ+CL+WE +  QY +  
Sbjct: 167 KLSSPFRCLSLKKFDV-PEEDIEHLQPTEVDDPYQDLETAYVAQLCLTWEALHCQYTQLS 226

Query: 409 DLWESEPHGLHHYNEVAGEFQHFQVLLERFLENEAFE-GPRVENYVKQRCVVRNLLQVPV 468
            L   +P     YN  A  FQ F VLL+R++ENE FE G R E Y + R  +  LLQ P 
Sbjct: 227 HLISCQPETPTCYNHTAQLFQQFLVLLQRYIENEPFEQGSRSELYARARNAMPKLLQAPK 286

Query: 469 IRANLVPFLIEDKTRDRRKARQWRDHENEAITNDMLVEILQESIRVISQFIRADKVHNPT 528
           I+ +       DK       ++        +  D L+++++ SI   + F++ DK   P 
Sbjct: 287 IQGS-------DK-------KEMEKDTGFMVLADDLIKVIESSILTFNVFLKMDK-KKPN 346

Query: 529 ATLKRPKKFQVELQDPTDMQLLTEIQVDLQKKERKVKEKMRSGHCILKK-LKKNEEEEET 588
             +           + T   LL +  +D  KK  K KE  +    + KK   +  E  + 
Sbjct: 347 GGIHLFGNHNNNHVNSTTPLLLVQSSID--KKRVKAKELSKKTKGLRKKSWPQTWEGVQL 406

Query: 589 EEHEMDMKLVGRVLKMSRITTDQLIWCRNKLSRIHFINTKIHLEPSFFLFPC 619
               +D+KL  RVL+MS+I+ +QL+WC  K+ +++F   K+   PS  LFPC
Sbjct: 407 LFAAIDIKLATRVLRMSKISKEQLLWCEEKMKKLNFSAGKLQRHPSPILFPC 437

BLAST of Carg22896 vs. TAIR 10
Match: AT1G73850.1 (Protein of unknown function (DUF1666) )

HSP 1 Score: 104.4 bits (259), Expect = 3.4e-22
Identity = 97/329 (29.48%), Postives = 156/329 (47.42%), Query Frame = 0

Query: 288 ELHSFYRSYRERMRKLDILNYQKMYAMGVLQSKDPLKSFSSNTKSSSPSITSVLRL--YR 347
           E ++ ++ Y E M  L  ++ QK      L   + LKS     +S S  I   L    ++
Sbjct: 313 ESYTVFQKYDEEMTFLTRISAQK------LHEAESLKSIMVEPRSISERIVHKLSSNGHK 372

Query: 348 QKKCQVDPMKDFIREVHCDLEMVYVAQMCLSWEFIQWQYGKALDLWESEPHGLHHYNEV- 407
           +K+ Q           + +LE  YVAQ+CL+WE + W Y K  +   S       +N+V 
Sbjct: 373 KKQKQYPGSNGSRPNPYVELESAYVAQICLTWEALSWNY-KNFERKRSTTQ--RSFNDVG 432

Query: 408 -----AGEFQHFQVLLERFLENEAFE-GPRVENYVKQRCVVRNLLQVPVIRANLVPFLIE 467
                A +F+ F +LL+R++ENE +E G R E Y + R +   LL VP           +
Sbjct: 433 CPAGIADQFRTFHILLQRYVENEPYEHGRRPEIYARMRTLAPKLLLVPE---------YQ 492

Query: 468 DKTRDRRKARQWRDHENEAITNDMLVEILQESIRVISQFIRADKVHNPTATLK----RPK 527
           D   +  K  +  +     I++   + I++E IR    F++ADK       +K    R K
Sbjct: 493 DYEEEEEKEDENEEGFRSRISSASFLMIMEECIRTFMNFLQADKEKPCQKIIKAFFGRSK 552

Query: 528 KFQVELQDPTDMQLLTEIQVDLQKKERKVKEKMRSGHCILKKLKKNEEEEETEEHEMDMK 587
           +  V   DPT + L+ ++     KK+ K+KE  R G  + KK    EEE E     +D+K
Sbjct: 553 RGPV---DPTLVHLMKKVNT---KKKTKLKEMRRGGKYMRKKKMSIEEEMEILMGLIDLK 612

Query: 588 LVGRVLKMSRITTDQLIWCRNKLSRIHFI 604
           +V RVL+M+ +  + L WC  K+S++  I
Sbjct: 613 VVSRVLRMNEMNEENLHWCEEKMSKVKII 617

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022223.10.0e+00100.00hypothetical protein SDJN02_15953, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022930474.10.0e+0096.79uncharacterized protein LOC111436904 isoform X2 [Cucurbita moschata][more]
XP_023529275.10.0e+0095.83uncharacterized protein LOC111792173 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022930467.10.0e+0096.75uncharacterized protein LOC111436904 isoform X1 [Cucurbita moschata][more]
XP_022970620.10.0e+0094.86uncharacterized protein LOC111469544 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1ERH80.0e+0096.79uncharacterized protein LOC111436904 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1EQK90.0e+0096.75uncharacterized protein LOC111436904 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1I3C60.0e+0094.86uncharacterized protein LOC111469544 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1HZL30.0e+0094.81uncharacterized protein LOC111469544 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1BVT27.3e-21464.08uncharacterized protein LOC111005168 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
Match NameE-valueIdentityDescription
AT5G39785.11.5e-11041.25Protein of unknown function (DUF1666) [more]
AT5G39785.21.4e-10841.03Protein of unknown function (DUF1666) [more]
AT1G69610.11.3e-6935.78Protein of unknown function (DUF1666) [more]
AT3G20260.11.9e-2528.88Protein of unknown function (DUF1666) [more]
AT1G73850.13.4e-2229.48Protein of unknown function (DUF1666) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 529..549
NoneNo IPR availableCOILSCoilCoilcoord: 554..574
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..191
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 166..191
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..53
NoneNo IPR availablePANTHERPTHR46741:SF2OS09G0413600 PROTEINcoord: 168..618
NoneNo IPR availablePANTHERPTHR46741OS09G0413600 PROTEINcoord: 168..618
NoneNo IPR availablePANTHERPTHR46741OS09G0413600 PROTEINcoord: 50..196
NoneNo IPR availablePANTHERPTHR46741:SF2OS09G0413600 PROTEINcoord: 50..196
IPR012870Protein of unknown function DUF1666PFAMPF07891DUF1666coord: 364..617
e-value: 1.7E-85
score: 286.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22896-RACarg22896-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane