Carg22869 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22869
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionvacuolar protein sorting-associated protein 52 A
LocationCarg_Chr15: 285039 .. 298257 (+)
RNA-Seq ExpressionCarg22869
SyntenyCarg22869
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAAAGGAAGATTGTTGAAAAGTAAATAGAAGCCATTCTCGCGGGTGCTACGTTGTTGACGATGCACAAGAACTGCTTGAATCTTCTCTCCGATAAAACAAGCAGTCTACAAATTCAATCCTCCGGAGAAGAATGACATTGCTCATTCTCTCGTCAACGTTGAAGAAATCCCCGACATCGAGTTGTTTTCATTATTTGATATCAATTTCGGAATTCTGAGGCGCAACTGAAGATTTGGAGAACTAAACGTCTCGCCGGCTCCGGTGTTCGAACCCCTCTCCGATCTGTAAAACCCTTGTCGAATTCTTCCATCTCGGCAGCTTCTCAAAACATAAGCTAGGTGTCGGGTCAGTGTGAATTTATCTAGTATATATCAGTGAAATGCAATTCTGTATTTGTGCTTACATTGATGCGTATCATTCTTTGATCTCGATGGTTATTTGCTTACTTGGTGAATGATTAAGTGATCGTTAACTGTGAATGTGAGAAGCTCAGGATGTTGGTATTTCCATGACCCGATTGGTTTATTGTAAATCTAGATCGCGCTGGCGGACTATCTGTTAAGCACTCTTGGCCAATTCGGACGGGATGGCCGATACTGCGATCGACCAAGTGGTATAGTAAACTTATATTCTTTTTAATATATCTTTAGCGCTTTTCGGTTGGAGTACCATAGAAGTAATCTCATTCGAGTTAGGATGAATCACGACCACGTTCCAAGTGGTACGATAAGTGGGAATGTTGGTATCAGCGATAAGATCTTTCAAAAGTGAAGATTATGAATGTATCTACGTTTAATAATGGAATGGAAGTTGAACTGCGTAGTGTTAGCTAATAAAAACTCGACTTCAATTCAGTTTTGGACAGCAATGTATTATATATTTTGTCATGCTTCTTTACGCACTGCAGAATCACTCTTATGATGAAGCCAACCAGACCCAGAAGAATGTTTTTGACCTGGAATCATTTGTTGGGGACTTGACTGTCGAGGAGGATGCTTGCAGGTAGGTCCATTGCTCATGTTTCTTATTCTTGTATGATCATGAGTTTATTTGGCTATTATATTTATTACCTTCTACTATTGAATTACCAAATTTGACTGTGGTATCAATTTATCCCGTTTTACAGTGAAGACATTTCATTGGAAGGACTGCAGCAAGAACTCGAAGAATGTAAAGATGATGGTGTAAGTTATTTCTAACTTTGGTGTAAAATTTGTATGATTCAGAGCTTTAGCTTTACAATCATAATCAGTGTGTTTTATATCCGGTGGTAGTGTCTTTTGCCCCCAACAGTTCTATTATTTTCAAATTTCATATATTATTGTACATTTCGACAATTAATAATTGATTTTATTGTAGTTTGTAAATGTGCAAATCTTCTTGTCACTTTTTTTTTAATTATTAATTTTTACTTTTTGTTTTTTTTGAATTATGGAAGTTGTTCAATTCTTTTAAGATAATGTTTCAAGATATTATTTCCTTTGTACAGGATCCCTTGTAGTGAACTTTTATTCATTTCTTGTATCGCCTGTTAGAATCTTTCACAACCTTACTTGGACATGATCTGCTCTATAGGTTGTACTACTATGTCGAGTTAATTTTTTTAGAATGTTTCATTCAGCTTAATGAAAGCTTGTTTTTTTTCATAAAAATAAAAATATATTTTTTTCTATAGGTATCAAATATATAATCTGTAAATAACGTGCCCAATCATTCGGTGTTATTGTACATTATGACTCATGGATAAAAAATCTTGGAAGAATTTCTAATGAAAACTGACTTTATTTTCCTTACTATTAATCTTCTTTGGTGCTTAGTTGCATTATTTTAGCTTCTTTATGGTCTTCTAGTATAGTTTGAAAACGAGGGGCTTATGGTTTCATTCTTCCCAGAATATGACATCTAGTCTGAAATTAAAATTGTCTATCGGTGATAGAAGTCTAGTACTGATAGACTATATATGGTATATTAGTGATGGACCTGAAAATTTTAAAAATTGACATATTTACGAATTACTTATTTGCATGACATATTTGCAAATACAACAGCTAATTTTGCCGCCCATTACAATCCTTTATTTTTTAACTCTTTATTAATCAGGTAGTTGCGAACATACTTTCTAAAGGTGTAAAACTAAGGGAGTACACAAAGGGAGTTGAAAACAATTTGCGGCAGGTTGAGCTGGATTCCATACAGGTTTGTCATCATTTCTAGTTCTTTTTTGTTTACATTTCTAGCTCTAAATATTTCTAATCTTTGCTATGCATTTCTTTTTAGAAGTGTATACATTATCCTGCCTTGCCTGTGATTAAGCAATGTTATAGACATCTATGTGTACTCTGTTTAACACTGCAAAAGTGCTTTGGCTATTGCTGTCTTTTGCTCTCATTAATATTTCTACTTTCAGAGACAGCTTTAAAGATGTAAAATGCCGCATGTCAGGAGTAGTTGATATGATTAATTAGAACAAGCTGGTTGCTAAGCATAATCTGGGTTGTTGGTTGGTTTAGAATTCCATGATCTATGTTTTTTTTTCCATGCCTCGAGATGGTATTTTAGCTATTTAATCGCAATTGTTCCCTTTTTATGATTAGCAGCACTTGTAGAGATCATTCTTAATCTTCTTTGACCCTGCATGTGGGGAATATCTTTATTTCCTTTCTCTTTGCATGTTGTCTCTTTTCAATATATTGTCTCTTATCTTGAAAAAAAAAAAAAAAAATGCTCAAGATCTATCTAGTTGAATTATATTGGTAATTTCACATAAACCTATGGACACGTATAACTGATATGCACACACTTAACTTGTTTGTGCAAACATGCGAAATATGCACATATATTTGTGGTTCATTAGTTCTATTATTGAGTGTTCTTCCTTTTAACATATCAATAAAGAGTTGTTCTTGTTAAATTGAAAAAAAAAAAAAGAAGATTCCAGTATTAAGGGCTCATTCTTATTGTTCATAAATTGCAGGAATATATTACAGAAAGTGATAACTTAGTCTCACTTCATGAACAAATTCGTGACTGCGATAACATTTTATCACAGATGGAAACTCTTCTCAGTGGTTTCCAGGTATTTTGGCTGCTGTTCTGGCACTCTAGTAATATCAAACTTCTTAGTGAAGTACATCAATCTGGTTGTATTAGCCTGTAGCACTGTTTTGTGTCATTATAACTTCTTTAAAGTCTGAATTTGTATCTAGTTCCTGTTTTCATAAGTTCGTATCTGATTATGCCTCGTTAAATGAGACTATGGGACTTATGTCATCTTTCTCAAATTTGAATGCATTTGGAATTACATTGACCTTCTTGAATTTAATTTCTATTGTTAATCTATGTCATCTTAAAGGAGTTTGTAATCATTGTTCCCCACCTCCCCCCTAAAATTTTCGTTGTTTCTGTTATTTAATTTTGTTCATCCTAGAGATTCTTTCCCAAAGAATTAATATATGTATATTTTTTAAAATTTTCAGGCCGAAATTGGTTCTATCAGTGCAGACATTAAAGTCTTGCAGGAGAAGTCAATGGATATGGGTCTGAAGCTCAAGAATCGCAAGGTATTCTTGGTTGGATTAAAAAAGTTCATTACTTTAGTAATTTGAAAAAACTGAAACAATTAAGTCTTAAGTCTCAATAGGCGTTTACAATGGCTTGCTTACTTGCAATATCCATTGCTGTTTAATTTAGCAACGTATCAGATACATTCTTATTTTCCAGAAACTTATAAGGACATTATTGTAGTTTTAAAGCACAACTATACTAATATAAAATGCTAAGAGATAGGAAGAAGTGAAATTAAGGTGCTATGATCGTTACTACGGCATTTTGGACTTCTTAACTATCATATTTTAGTAAGTTAGTCTTTCTTCTTTAATTTACAATTGGAGATCATTTGTGTAATCCACCATTGGTGGTCATTTTATTCATCGATGAAATTCTTGTTTCTTCTTCTTCATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAGAAAAAAAGGGAGAGAGTATTTTTTGATAATATTAGTTAGTTATAATGTTCGTTAGGAGGAACTATAAGTTAACTAAATACATGAGCACTATGCATTTGACATTGATTTTGAGTAGTAGTTCAAGACTTAAAATGTTTTCCTTTTCTTTTTGTCCCCGAGAACAAGCTTTTCGCAACTTTGCTTATTCTCTAATAAGACTGCTATATTTTATTTGTGATATTATGGTGTTCGTCTCTAGTTTATTTTAGAAATCATTTTTCATTCTAGGCGTTGCTTTTCAGGTGGGAACTAGCATATTGTTTTTTAGATAGTATGCGCTTTCTCATCTGTTACTCTGAAAGTTGTTAATGATACCTTTTTGGGCTTAGCTTTCCTCCCCTTGTGTAATTTTATATTAACTAGGAAATAATCATTTTCTTATAAAAATAGGTTGTTTTTGGAACCTGAAATATTGGGGGCTCAGTAAATGCATTTATGGGGTCCTGAAAAGTTTCTTTTTTCTTCCTTTTTTCTCTTTAATACTTATTTTAGCGAGACTTCATGTGAATGCATATAGCAGTTGGTTTCTTTTGAAAAGAAAATTGCATGTTCATCTAGTTTGCTCATTATTGGAACTCAGTAAATGCGTTTATGGGATCCTGAAAAGTTCATTTTCTTTCTTCTTTCTTTTTAATGCTTCTGTAAGTGAGAATTCATGTGAATTCCTGTAGCAGTTGCTTTCTTTTAAAAAGACAAATTGCATCTTTGTTTAGTTTTGCTCATTATTTTAACTCAGCATAATACATACAGCACTATTCTGTCTTTCTACCCTATCATTTTTTTTTTCTCATGTGCTAGGCTTTATGTTCTTATTCAGGTGGCAGAATCAAAATTGGCAAAGTTCGTCGAAGAGATCATTGTTCCTCCAAGAATGATAGACATAATTGTTGATGAAGAGGTATTGTTGGTATCGGTACATTTCACTTCTTCCATTTGAAGTTTGGCATTTTTTGTTACTCATAATTGTTCTTTCTCTAAATTTGTTTTGGACACCTTTAACTCTTTGGAGTTTCTGCTGTATGTCGTTCTTATTAATTCAGTTTTTCTGTTTGCAACTAAAAAGGTATATATTTTGAGTAGTATTGAATAAATTATACTCATTTTAATAGAAGGGAGCTAAATTATCCCTTCTTCAATTTGGTTTTCATAATTTGTGATGCATAATTTGAAATTTCTTCATGCTTCCTTCTCTCTCTCTCTCCCCCCCCAATTTCCCCCCCACCCACTTTTTTGTTATTGGAATGCCTATGTTGGGATGTTTCAGTGAACCTTTGTTCCTTTCTTCAGGTGAATGATGAATATTTAAGAACTCTTGAGATTCTAAGTAAGAAGCTGGTGGTCGTGGAAGTGGATCCAATGATAAAAAATTCCAAAGCGCTTAAGGATGTCCAACCGGAGCTTGAAAAACTCCGACAGAAAGCAGTGTCTAAGGTTGTTCTCTGTCTCACAGCAGCCATATTCCTTTGAAAATAAATGTTAGTCTGTGTTTTCTTTGTGATATTTATAAAGTAGTATGTTATCTACTCCAACATCATTCACAGGGCTCTTGTAGTACAGCAGTAGTTAAATATGTAGAAAAACCATTAAATTTGAAATCACCTTCCATTTTTACTGTGAATAGCTTTTATAACACCAGAAATGACTTTGGTATTTTAGAAACAATATACAATTTTTTCCCCTCTTTCTGTTTTAATTTGTTTAAGAATGAAAATGTCAGTTCTGAATTGATTAGGCATATAACATAATTGACAGACTAGGAATATAACATGAGGAAAACTGACAAGGTATTACTATTCTGTTATATTTCTTGAAGTCTGGTAGGTCATGTATGCCTATTTGGATTTATCCATCATTCCAATCGATAATGTTCAGATGAGATTGATGTCTTGATTATCTCATATTAGAGATGAAAGATGTGGTCACCATCGATTGAACAATAAATTATAAATTTTGAATTGGTGCAGCTAGGACTCCAATTTAAGTGCATATGTATCAGTTATTGAATTTCTGTTTCTTTTTTGTTATTTTGACAGGATATTATTGAATAATTATACATTTTGTTTATGTTATAATTTTTCAGGTCTTTGACTTCATTGTCCAGAAACTCCATGCTTTGAGAAAACCCAAGACAAATATTCAGATCCTTCAACAGAGTGTTCTTTTGAAGTACAAGTAGGTCTAAAATTTTCCACTTTCCATACTTCCCTTTTCATTGTAGTCTGATGAAAAGACGCCAAGTATGGAATAAACAGATATTCAGAATGTTAGTACACCTTCCACTAACTTTCAGTACATATATTTTTCAAAGGAAGAGATAGAATTATAAAATAATACATTACAAAACAAGGGAGGTTAAGACATTCTTCCCAGCCAAAAAAACACGTAGAAGATTTTCAATAAACTTGCGTCAGAAAACAATTATGATCATGAATGGCTTAATCAAGATAATCTTTAAAAATCTGCAATCTACTCACCATCAAGACAGCGATATGATTGCATCTGCAATCTATTCACCATCAAGACAGCGATATCCAAGAGAAACCAGACTTGCCAAAGATAACGCCAGCCACAATGAGCTGTGAACTCATGGCCTTGTTCTTTAATCCATGAACAACAAAAGGTTATGGAGGAGCCACCCTTCAAGACCACTGGGATGATAAAAATTCCAAATTTAAAGACCAAGCTAATTGTCTAGGATTCCTGCCTTGCCATCGCTTGTCATGCATGTATCTGGGGGAGGATATTAATATGGATTCACTTTTGGGTTCCATATACCATTTATGTCTTGCAATTAGAGAGAGTTAAGTTGATTTGGCAAACATTCAGGTTGATCTAACAGAATTTCGGACATGAGTTTATACATCATGCACCCTATTTCGTCAATCTTCCATTTCTTCTATCTTTCATATTGCATGGCCTGCTATTGTCAAAAATTGTGCTAAGAAACCACTTCTTTCCTCCCTCTCATAAAAGTTTCCATTGAAGTTTAGTTTTTCAGCTTTGCTCCAACTCATTCCACATGTCGTTCCAGTGCCCTGTTGTTTCTCTTACTCAGGGGTATGCGTATAGCACCCATAAAATAGATTTTGCGGTCCTCCCTGCCTTTCCACTTATTATTACATAAGAAAATTTGTTACCATCTTCCAGTCTGATTCAGAAATTAGGATGTTGTTTTCCTTTTTCTACATGTAATTTAGAGAGCAGATTCACAAACTATACTATTGCTTGCTGAATATTTTGATGTGTTTCTTCTTCTATTAGTAGTTGAATTATTCATTGATTGTTCTTTGTTTTTATAATTATAATGTGTGTCTTTTATGCACAATGAAGTAGTAATCAATACGTGCCTCAACAACGATCCATTTATTGCTTACCCTGGTATTTTTCATCCATGAAATTCAATATTATTCCATGATTTTGCATCTCTTTTTATCTACCCGAGGTTTATCATTGAATATCTTTTCAGATTCTTCTACCTTAATGATAATGTAGGTATGTTATTTCTTTCCTCAAGGATCATAGCAAAGAAGTTTATCTTGAGGTTCGGACAGCGTATATCGATACAATGAACAAGGTAATTGTAGAAGAAATCATTTATTCTTTTAATATCATGCATGGTTTAATTACATGAACCTCTGTGGAAGACATGCCAATTCAAATCCTTGGACAAAATATTACTCTTAATATTTGATCATTATTTTGAGTTGTTTCTATGTACGGGAAATTCCTGGTTATCTTGATTTATTTTGACCTGGGAGTTCCAAATCATTGATGCTGGCATGATGCAAGTTGACATACAATGCTAACAATCTACTGCCAGTTGAGCGTAACAACTCACGTTGGACTTATCAGACTTATCAGAACACTAAAATGTGACATGACATTGAAGGAGTCCAAAAGTTAGAGCTTGTGTGGGTTACTTCAGTGTGGATTTATTAGACTTATCAGAATACTGTTATTTCTTAAACAAGTAACAGAAAAGAAAATAAAAGGCAACAATCCGTAGCATTACATCAGTGTGGGTTCCACAAATCATTCTCGATATTCTCTTGAGTGCTATGAGGGCTCAAAGTATAAAAATGGTCCAAGGGATGATGATTATTTTCCTATTCAAGAGACTTGAACATAATACAAAGAACAAATCTTCTACAAGCTTCAAGTCTATGAATATTCTCATGACTTATTTTGGAATGTATATGGCAGTACGTTGTCATTGTCACTATTCCAATGCCTATTCAATTGAAGATGAGAATAAATATTTAGAGTATTTATGGGACCAACAATAATTAGTTTTTGATCTCTTTATAATGATTACTCAGCATGCATTATTTGGCCTTTATATATTGGCTTTTCAACATTCCACGTGACAATTTTTCATGCATCAATAAAAATTGGTTCCTGTTTTTAAAGGGAAAAACATTTGTCTATGAGTGCTGAGTTATTGATGTACTAAAACTAGTATTCATGTTTTTCAGGTTTTGAGTGCACATTTCCGTGCCTACATACAGGCACTAGAGAAACTACAGTTAGACATAGCGACATCTAGTGATCTGATTGGCGTCGAGGCTAGAAGTTCAGGTCTTTTCTTGAGAGGAAGAGAACCACTGAAGAACCGATCTGCTGTTTTTGCACTTGGTGATAGAATAAAGATATTGAAGGTTTTATTACTCTCTTAACTCTTGTAACCTTCTCGATCACGTTATTTTCTTACTCTTGTTTGAATTCTAACTTGTGACTTCTTTACTCTCATAGTTCTTCATTTCTTCATTCTTGTTTGAACTAGTATCTGAGTCTTGTTGACCAACTGTTTTTATGTGAAGTTGTGTATTTGTTTTTGGTTAAATTACAAATTTAGGCCTTGAACTTTAAGGATTGTGTCTGATAGGTTTGAATTTTGAGAAGTGTTTGATATGTTCTTGAACTGCCAATTTTAAGTCTTGACATGCAGACATTTTTCAAAATTAATAGATCTATGGATTTTGTGTATAATGTGACTGAAGCTTTCAAATTTTTCTGTCTAATAATACGTGGATTTAAAATAGGTCAAATACCTATTAGACTCCAAATTGAAAGTTTTGGGACTTGCTAGCCTCTATTTTTTTTATTCCTTAGTTTTTAGATTATACTTTCATTTTGATGTACTAGAAATAGATGATTTAAATTATCATCTTTTATTAAAGAAGTATTTGAACTTAGAACAAACGAGTTTGGGTACATTCACATGTGTTAAATAACATCTGTATATAAGTTTGCATTACTAATTGTTCTTGTTACATGTCCCGGTGTGAACTCCATTCAGGAAGTTGACGAACCTGCACTGATTCCACATATAGCTGAAGCCAGTTCAATTAAGTATCCTTACGAAGTTCTCTTTAGGAGCTTACATAAGCTTCTAATGGACACTGCCTCTTCTGAGTACTTCGCATTTTACTCTTCCTTTGTTTATTATCTTCAGAATGTTATTTGTAGCAAGTTTGTCAATTTTCTAATTAATTCTTTTCACAGGTATAATTTTTGTGATGAATTTTTTGGGGAACAATCTATGTTTTATGAGATCTTTGCGGGTAATTATGATAGAATCCTTGGCTTATTGTCAGTTAACTATCTGTACAGACGATATGTTGCAATATGAGTTTGAATATTTTATTACGAGTTTTCACCTTTCAATAAAGTTGTGCTATTGGTCTTATATTCCTTTTTTTTTTCAAATTTATAATGCATATTAATGCAAAAGCATCATTTTTTTACTTTTATTCTAAGGAAAAGATATGGAATGAAAGATACTTCATTTTCTATTATTATTGTGATTTGGTGTGGTTAACATGGTTTGAAATTCGTTCTCAAATAATCAGGTCCTTTTGCTGTCATCGATGAATACTTCAATTCAATTCTTCCAAACAGTTATGATGCAATTGGTCTAATGCTCATGATTCTGATCATTCATCAGCATCAGGTACATGATCTTATCTTAATCTGGTTTTAATTACATGGCTTCTCAATAAACTAAGGAATTGCTTCTAGGCTTTTATTTTAGTTCAATTAGGGAAATAGGCCCTAACTAGAAGATATTGGGAGGAAGATATGTCAAGATGGAGCTTACGATTTAGATCATTTTCTGAAATTTCTAACTATTTAAATTAACTCATCAAGTTGACTTCTTTTTCAGTTTTAAGAGCATGCCTTCCCTGATAATTATGTATTTTAAACTTTAAAGAATTACTTTTTTTCTTTTTCTTTTCAAAGGATGAAGTACGTAAGATGTCCCACAATCATTGATTAGTTTTAGAGTTGCATAACTTGTTAGGATATGTTAGATACTTGTTCTAGAAAAGCCTGCTAATGCAACTTGGTTATGACATTTATTTGCGACATATTCAGTTGCTTTTTTAAGATAAGAATCATGTATTCGGTTTTCTATTTGATCTTTTCTCTGCTTTCCAACTTGTTCAATATCTTGCAACACATCTACAACCTCTTTCACTTCGTTGCATCTTTTATTACCTACGATATATCCATTTGCTGACAAAGCTTATATATCCAGCTTATCATGTCACGGAGGCGTATACCTTGCTTGGATTCATATTTAGACAAGGTCTGTGTTTCTTGACAAGTTTAGACATCTGCTTAAATTTCTAGATGTCAGTTACATTATTCTCAATGGTGTTAGTTGATGGCCCCCTACGGTTTACATGGTCTATGATCGAGAACCTCCTCTATGTGTGAGCTTTCTATAGTTACTAAAATAACTGCGGAACTGATTTGCTGAATTGGAAATTGAAAGAAGAGAGAATTTTGAACTTCAATGTTTAAATGTTATATTTCTTTCAAATCCAAAGTTGCGTGATAACTGTATTGCTTGCTTATGAACTGCATAACCTGGTTGTTGGATTATCCTAAGCAGGTTAATATTGCTCTTTGGCCTCGTTTTAAGATGGTATTTGACATGCACCTCAGCAGTCTGAGGAATGCGAATGTAAAGACGTTGTGGGAAGATGATGTGCATCCTCACTATGTCATGAGGCGATACGTTGAGTTTACAGCTTCACTTATCCATCTGAATGTTGAATATGGAGATGGACAGGTCAGTCAGAGGCACAATATTGGGATACCTTTACTTTTTTTAATCAAGTCTTCACTGTCTAACTAGGTTGATACTTATTGTGTATATGCAATGTCATGTTTGCAGCTTGATTTAAACTTGGAGCGGCTAAGAATGGCAATTGATGATTTGCTTATCAAGCTTGCCAAAGCCTTTACAAAAGCTAAATCGCAGACAGTGTTTCTTATAAACAACTATGACATGACAATTTCCGTCTTGAAGGTAGGGTTTGGCTCTCAAGGTCTCTCTTCTAATGAAATCAATGAAGTAAAAAGACAGTAGGTAATGTCAATCCTGTAATTTCACAAAAGCTAGAAAGACAATCCCAAGAGGGTAACCTATCCTCATTATGGCTTGAAGTCTTTTTTCTATTAAATCTGCAGTTGCGCTCCATTTCCTTTTAAAAAAGAAGAAGGGTGCTTCCAAGAGTTCATTTGAAAGAAATGGTCTTCACGTAAATTTAAAGCCCTCATTAAGAAAATAGAAAGAACTTCTTGGACAAGATTATTTGTTCAAATTTTCCTTTTGGCGGGACAGCACGTGAGTTTGTTCATGGCTCTACAGAATCTCCTTCAATGCGAAATTTTATTGGTGTTACTTTGATTTCAGGAAGCTGGTCCGGAGGGTGGGAAAATTCTGGTGCACTTCGAGGATCTGCTGAAGAGCAACACAGCCTTGTTTGTGGCAAGTAACTTGCTGATTATAATATCCTAGAATGGTTCTAAAATATAGATCGCATTATTGATATTGATATTTGATTGCAGCTGCAGTTTATAGCTAAAATTGCTTAACCTCCATTCAGTCGTGTTCTTAATGCTTTACTTTTTAATTGAATGAAAAAGATTGGTGATTTGTGAGATGCGGTCCAGCTACCACTAAAGTGTCTGGACCCATCAAAAGATATCAACTGTACCTATTTGATCTGCACATGCCTTCTGCTTCACGCTTTTTTTTTTCCATAAAATTTGCATAAAAATATGATGTCCTAGAATGGCTTGTCATTCTTTTCGAGTCATTTAAAGATATAATACCTTCATGCAGGAAGAACTCTTAGCAGAGCATTTCAGTTCACTTATCAAGTTCGTTAAAACCCGTGGCTGTAAGTACTTGAACAAAGTTTACTTTGACTCCGTCGGTTATTAAAGTAACGAACTTATCAGAAATTCTCCTTTTCAGCGGAGGACCTAAGTTCTAATTCCGACCGACCAATTACTGTGGCTGAAGTAGAGCCCCTTGTAAAGGACTTTGCGAGCAGATGGAAAGCTGCAATTGAACTGATGCACAAAGATGTCATCACTTCTTTCAGTAATTTCTTGTGTGGAATGGAAATCCTAAGGGCGGCTTTGACTCAACTCCTCCTTTATTACACAAGACTCACGGATTGTATAAAGAGGATCGTTGGTGGCTCCGCTCTAAACAAGGATCTAGTGTCCATTTCTTCAATTATGTATGAAATTAGGAAATATTCAAGAACTTTTTAAAGTTGAGGAATCAGAACTGAGAGCAAGAAATGAAGATAGCATAATGTGTAAGCAAAAGCAGAAGCCAAAATCAGTACCTTCTTTTCTGAGCTTTCTTTTTGTGCATCACATTTCCATTCACAAAATGTTAAAGTGCTAGTATTCTTATTGCAGGCGCCAATGCAGCCGTGTTCGTGTTCAGTAATGCACAACTGCTTTCCTTTTCAGTTCTTTGGAAAATCTCTGGTCGATGCTCGAGGTTGTGTTGTGCCTTCACTCATCTATCACATTTCAGTGCCAAGGCATTATCATTAGTTAATTTGCTGTGTACAACTTACCATGGAACCTAATACACAACCCTTTCCCCTAAATCATCACCCCCACCAGAAAATCGTAGCTGGTGTATCACATTTTGTTGTAAGATTGTGTTCCAAATTAGAGGAAAAATAGAAACAATGGCTTTATATTGGGGTCCTAAGGTTTTGCCTTGAACTTGGAACGGACAGTCAGTTGGTGCGGACAGGTTAACATTTTATGGTTTTTCTGAGGTGTTACTGTATAATCACACACTTCTGTTTCTCAATGGTACGACATGTCGAGCATTGCTTACTGATTATC

mRNA sequence

GGGAAAGGAAGATTGTTGAAAAGTAAATAGAAGCCATTCTCGCGGGTGCTACGTTGTTGACGATGCACAAGAACTGCTTGAATCTTCTCTCCGATAAAACAAGCAGTCTACAAATTCAATCCTCCGGAGAAGAATGACATTGCTCATTCTCTCGTCAACGTTGAAGAAATCCCCGACATCGAGTTGTTTTCATTATTTGATATCAATTTCGGAATTCTGAGGCGCAACTGAAGATTTGGAGAACTAAACGTCTCGCCGGCTCCGGTGTTCGAACCCCTCTCCGATCTGTAAAACCCTTGTCGAATTCTTCCATCTCGGCAGCTTCTCAAAACATAAGCTAGGTGTCGGATCGCGCTGGCGGACTATCTGTTAAGCACTCTTGGCCAATTCGGACGGGATGGCCGATACTGCGATCGACCAAGTGTTTTGGACAGCAATGTATTATATATTTTGTCATGCTTCTTTACGCACTGCAGAATCACTCTTATGATGAAGCCAACCAGACCCAGAAGAATGTTTTTGACCTGGAATCATTTGTTGGGGACTTGACTGTCGAGGAGGATGCTTGCAGTGAAGACATTTCATTGGAAGGACTGCAGCAAGAACTCGAAGAATGTAAAGATGATGGTGTAGTTGCGAACATACTTTCTAAAGGTGTAAAACTAAGGGAGTACACAAAGGGAGTTGAAAACAATTTGCGGCAGGTTGAGCTGGATTCCATACAGGAATATATTACAGAAAGTGATAACTTAGTCTCACTTCATGAACAAATTCGTGACTGCGATAACATTTTATCACAGATGGAAACTCTTCTCAGTGGTTTCCAGGCCGAAATTGGTTCTATCAGTGCAGACATTAAAGTCTTGCAGGAGAAGTCAATGGATATGGGTCTGAAGCTCAAGAATCGCAAGGTGGCAGAATCAAAATTGGCAAAGTTCGTCGAAGAGATCATTGTTCCTCCAAGAATGATAGACATAATTGTTGATGAAGAGGTGAATGATGAATATTTAAGAACTCTTGAGATTCTAAGTAAGAAGCTGGTGGTCGTGGAAGTGGATCCAATGATAAAAAATTCCAAAGCGCTTAAGGATGTCCAACCGGAGCTTGAAAAACTCCGACAGAAAGCAGTGTCTAAGGTCTTTGACTTCATTGTCCAGAAACTCCATGCTTTGAGAAAACCCAAGACAAATATTCAGATCCTTCAACAGAGTGTTCTTTTGAAGTACAAGTATGTTATTTCTTTCCTCAAGGATCATAGCAAAGAAGTTTATCTTGAGGTTCGGACAGCGTATATCGATACAATGAACAAGGTTTTGAGTGCACATTTCCGTGCCTACATACAGGCACTAGAGAAACTACAGTTAGACATAGCGACATCTAGTGATCTGATTGGCGTCGAGGCTAGAAGTTCAGGTCTTTTCTTGAGAGGAAGAGAACCACTGAAGAACCGATCTGCTGTTTTTGCACTTGGTGATAGAATAAAGATATTGAAGGAAGTTGACGAACCTGCACTGATTCCACATATAGCTGAAGCCAGTTCAATTAAGTATCCTTACGAAGTTCTCTTTAGGAGCTTACATAAGCTTCTAATGGACACTGCCTCTTCTGAGTACTTCGCATTTTACTCTTCCTTTGTTTATTATCTTCAGAATGTTATTTGTAGCAAGTTTGTCAATTTTCTAATTAATTCTTTTCACAGGTATAATTTTTGTGATGAATTTTTTGGGGAACAATCTATGTTTTATGAGATCTTTGCGGGTCCTTTTGCTGTCATCGATGAATACTTCAATTCAATTCTTCCAAACAGTTATGATGCAATTGGTCTAATGCTCATGATTCTGATCATTCATCAGCATCAGCTTATCATGTCACGGAGGCGTATACCTTGCTTGGATTCATATTTAGACAAGGTTAATATTGCTCTTTGGCCTCGTTTTAAGATGGTATTTGACATGCACCTCAGCAGTCTGAGGAATGCGAATGTAAAGACGTTGTGGGAAGATGATGTGCATCCTCACTATGTCATGAGGCGATACGTTGAGTTTACAGCTTCACTTATCCATCTGAATGTTGAATATGGAGATGGACAGCTTGATTTAAACTTGGAGCGGCTAAGAATGGCAATTGATGATTTGCTTATCAAGCTTGCCAAAGCCTTTACAAAAGCTAAATCGCAGACAGTGTTTCTTATAAACAACTATGACATGACAATTTCCGTCTTGAAGGTAGGGTTTGGCTCTCAAGGAAGCTGGTCCGGAGGGTGGGAAAATTCTGGTGCACTTCGAGGATCTGCTGAAGAGCAACACAGCCTTGTTTGTGGCAAATATAATACCTTCATGCAGGAAGAACTCTTAGCAGAGCATTTCAGTTCACTTATCAAGTTCGTTAAAACCCGTGGCTCGGAGGACCTAAGTTCTAATTCCGACCGACCAATTACTGTGGCTGAAGTAGAGCCCCTTGTAAAGGACTTTGCGAGCAGATGGAAAGCTGCAATTGAACTGATGCACAAAGATGTCATCACTTCTTTCAGTAATTTCTTGTGTGGAATGGAAATCCTAAGGGCGGCTTTGACTCAACTCCTCCTTTATTACACAAGACTCACGGATTGTATAAAGAGGATCGTTGGTGGCTCCGCTCTAAACAAGGATCTAGTGTCCATTTCTTCAATTATGTATGAAATTAGGAAATATTCAAGAACTTTTTAAAGTTGAGGAATCAGAACTGAGAGCAAGAAATGAAGATAGCATAATGTGTAAGCAAAAGCAGAAGCCAAAATCAGTACCTTCTTTTCCGTGTTCGTGTTCAGTAATGCACAACTGCTTTCCTTTTCAGTTCTTTGGAAAATCTCTGGTCGATGCTCGAGGTTGTGTTGTGCCTTCACTCATCTATCACATTTCAGTGCCAAGGCATTATCATTAGTTAATTTGCTGTGTACAACTTACCATGGAACCTAATACACAACCCTTTCCCCTAAATCATCACCCCCACCAGAAAATCGTAGCTGGTGTATCACATTTTGTTGTAAGATTGTGTTCCAAATTAGAGGAAAAATAGAAACAATGGCTTTATATTGGGGTCCTAAGGTTTTGCCTTGAACTTGGAACGGACAGTCAGTTGGTGCGGACAGGTTAACATTTTATGGTTTTTCTGAGGTGTTACTGTATAATCACACACTTCTGTTTCTCAATGGTACGACATGTCGAGCATTGCTTACTGATTATC

Coding sequence (CDS)

ATGCTTCTTTACGCACTGCAGAATCACTCTTATGATGAAGCCAACCAGACCCAGAAGAATGTTTTTGACCTGGAATCATTTGTTGGGGACTTGACTGTCGAGGAGGATGCTTGCAGTGAAGACATTTCATTGGAAGGACTGCAGCAAGAACTCGAAGAATGTAAAGATGATGGTGTAGTTGCGAACATACTTTCTAAAGGTGTAAAACTAAGGGAGTACACAAAGGGAGTTGAAAACAATTTGCGGCAGGTTGAGCTGGATTCCATACAGGAATATATTACAGAAAGTGATAACTTAGTCTCACTTCATGAACAAATTCGTGACTGCGATAACATTTTATCACAGATGGAAACTCTTCTCAGTGGTTTCCAGGCCGAAATTGGTTCTATCAGTGCAGACATTAAAGTCTTGCAGGAGAAGTCAATGGATATGGGTCTGAAGCTCAAGAATCGCAAGGTGGCAGAATCAAAATTGGCAAAGTTCGTCGAAGAGATCATTGTTCCTCCAAGAATGATAGACATAATTGTTGATGAAGAGGTGAATGATGAATATTTAAGAACTCTTGAGATTCTAAGTAAGAAGCTGGTGGTCGTGGAAGTGGATCCAATGATAAAAAATTCCAAAGCGCTTAAGGATGTCCAACCGGAGCTTGAAAAACTCCGACAGAAAGCAGTGTCTAAGGTCTTTGACTTCATTGTCCAGAAACTCCATGCTTTGAGAAAACCCAAGACAAATATTCAGATCCTTCAACAGAGTGTTCTTTTGAAGTACAAGTATGTTATTTCTTTCCTCAAGGATCATAGCAAAGAAGTTTATCTTGAGGTTCGGACAGCGTATATCGATACAATGAACAAGGTTTTGAGTGCACATTTCCGTGCCTACATACAGGCACTAGAGAAACTACAGTTAGACATAGCGACATCTAGTGATCTGATTGGCGTCGAGGCTAGAAGTTCAGGTCTTTTCTTGAGAGGAAGAGAACCACTGAAGAACCGATCTGCTGTTTTTGCACTTGGTGATAGAATAAAGATATTGAAGGAAGTTGACGAACCTGCACTGATTCCACATATAGCTGAAGCCAGTTCAATTAAGTATCCTTACGAAGTTCTCTTTAGGAGCTTACATAAGCTTCTAATGGACACTGCCTCTTCTGAGTACTTCGCATTTTACTCTTCCTTTGTTTATTATCTTCAGAATGTTATTTGTAGCAAGTTTGTCAATTTTCTAATTAATTCTTTTCACAGGTATAATTTTTGTGATGAATTTTTTGGGGAACAATCTATGTTTTATGAGATCTTTGCGGGTCCTTTTGCTGTCATCGATGAATACTTCAATTCAATTCTTCCAAACAGTTATGATGCAATTGGTCTAATGCTCATGATTCTGATCATTCATCAGCATCAGCTTATCATGTCACGGAGGCGTATACCTTGCTTGGATTCATATTTAGACAAGGTTAATATTGCTCTTTGGCCTCGTTTTAAGATGGTATTTGACATGCACCTCAGCAGTCTGAGGAATGCGAATGTAAAGACGTTGTGGGAAGATGATGTGCATCCTCACTATGTCATGAGGCGATACGTTGAGTTTACAGCTTCACTTATCCATCTGAATGTTGAATATGGAGATGGACAGCTTGATTTAAACTTGGAGCGGCTAAGAATGGCAATTGATGATTTGCTTATCAAGCTTGCCAAAGCCTTTACAAAAGCTAAATCGCAGACAGTGTTTCTTATAAACAACTATGACATGACAATTTCCGTCTTGAAGGTAGGGTTTGGCTCTCAAGGAAGCTGGTCCGGAGGGTGGGAAAATTCTGGTGCACTTCGAGGATCTGCTGAAGAGCAACACAGCCTTGTTTGTGGCAAATATAATACCTTCATGCAGGAAGAACTCTTAGCAGAGCATTTCAGTTCACTTATCAAGTTCGTTAAAACCCGTGGCTCGGAGGACCTAAGTTCTAATTCCGACCGACCAATTACTGTGGCTGAAGTAGAGCCCCTTGTAAAGGACTTTGCGAGCAGATGGAAAGCTGCAATTGAACTGATGCACAAAGATGTCATCACTTCTTTCAGTAATTTCTTGTGTGGAATGGAAATCCTAAGGGCGGCTTTGACTCAACTCCTCCTTTATTACACAAGACTCACGGATTGTATAAAGAGGATCGTTGGTGGCTCCGCTCTAAACAAGGATCTAGTGTCCATTTCTTCAATTATGTATGAAATTAGGAAATATTCAAGAACTTTTTAA

Protein sequence

MLLYALQNHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKGVKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEIGSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRTLEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIATSSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPYEVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQSMFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVNIALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLDLNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSGALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEPLVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSALNKDLVSISSIMYEIRKYSRTF
Homology
BLAST of Carg22869 vs. NCBI nr
Match: KAG7015901.1 (Vacuolar protein sorting-associated protein 52 A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 749/749 (100.00%), Postives = 749/749 (100.00%), Query Frame = 0

Query: 1   MLLYALQNHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVV 60
           MLLYALQNHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVV
Sbjct: 1   MLLYALQNHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVV 60

Query: 61  ANILSKGVKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLL 120
           ANILSKGVKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLL
Sbjct: 61  ANILSKGVKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLL 120

Query: 121 SGFQAEIGSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEV 180
           SGFQAEIGSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEV
Sbjct: 121 SGFQAEIGSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEV 180

Query: 181 NDEYLRTLEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALR 240
           NDEYLRTLEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALR
Sbjct: 181 NDEYLRTLEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALR 240

Query: 241 KPKTNIQILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEK 300
           KPKTNIQILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEK
Sbjct: 241 KPKTNIQILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEK 300

Query: 301 LQLDIATSSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEA 360
           LQLDIATSSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEA
Sbjct: 301 LQLDIATSSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEA 360

Query: 361 SSIKYPYEVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCD 420
           SSIKYPYEVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCD
Sbjct: 361 SSIKYPYEVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCD 420

Query: 421 EFFGEQSMFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLD 480
           EFFGEQSMFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLD
Sbjct: 421 EFFGEQSMFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLD 480

Query: 481 SYLDKVNIALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVE 540
           SYLDKVNIALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVE
Sbjct: 481 SYLDKVNIALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVE 540

Query: 541 YGDGQLDLNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWS 600
           YGDGQLDLNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWS
Sbjct: 541 YGDGQLDLNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWS 600

Query: 601 GGWENSGALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPI 660
           GGWENSGALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPI
Sbjct: 601 GGWENSGALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPI 660

Query: 661 TVAEVEPLVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIK 720
           TVAEVEPLVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIK
Sbjct: 661 TVAEVEPLVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIK 720

Query: 721 RIVGGSALNKDLVSISSIMYEIRKYSRTF 750
           RIVGGSALNKDLVSISSIMYEIRKYSRTF
Sbjct: 721 RIVGGSALNKDLVSISSIMYEIRKYSRTF 749

BLAST of Carg22869 vs. NCBI nr
Match: XP_022938844.1 (vacuolar protein sorting-associated protein 52 A isoform X1 [Cucurbita moschata] >XP_023550700.1 vacuolar protein sorting-associated protein 52 A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1259.2 bits (3257), Expect = 0.0e+00
Identity = 675/742 (90.97%), Postives = 677/742 (91.24%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG
Sbjct: 10  NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ
Sbjct: 190 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY
Sbjct: 310 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRSLHKLLMDTASSE                              YNFCDEFFGEQS
Sbjct: 370 EVLFRSLHKLLMDTASSE------------------------------YNFCDEFFGEQS 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLK               +G
Sbjct: 550 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLK--------------EAG 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
              G        +         EELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP
Sbjct: 610 PEGGKILVHFEDLLKSNTALFVEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. NCBI nr
Match: XP_022994068.1 (vacuolar protein sorting-associated protein 52 A-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1257.7 bits (3253), Expect = 0.0e+00
Identity = 674/742 (90.84%), Postives = 677/742 (91.24%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG
Sbjct: 10  NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           L+ILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ
Sbjct: 190 LKILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY
Sbjct: 310 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRSLHKLLMDTASSE                              YNFCDEFFGEQS
Sbjct: 370 EVLFRSLHKLLMDTASSE------------------------------YNFCDEFFGEQS 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLK               +G
Sbjct: 550 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLK--------------EAG 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
              G        +         EELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP
Sbjct: 610 PEGGKILVHFEDLLKSNTALFVEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. NCBI nr
Match: XP_038884385.1 (vacuolar protein sorting-associated protein 52 A isoform X1 [Benincasa hispida])

HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 652/742 (87.87%), Postives = 665/742 (89.62%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           N SYDEAN+TQKNVFDLESFVGDLTVEEDACS+DISLEGLQ ELEECKDD VV NILSKG
Sbjct: 10  NQSYDEANKTQKNVFDLESFVGDLTVEEDACSDDISLEGLQLELEECKDDNVVTNILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYI ESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYIKESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVD EVNDEYLRT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDGEVNDEYLRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKV+DF+VQKLHALRKPKTNIQ
Sbjct: 190 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVYDFLVQKLHALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVY EVRTAYIDTMNKVL AHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYNEVRTAYIDTMNKVLGAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY
Sbjct: 310 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRS+HKLLMDTA+SE                              YNFCD+FFGE+S
Sbjct: 370 EVLFRSMHKLLMDTATSE------------------------------YNFCDDFFGEES 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MFYEIFAGPFAVIDE+FNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFYEIFAGPFAVIDEHFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRY EFTASLIHLNVEYGDGQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYAEFTASLIHLNVEYGDGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAK F+KAKSQTVFLINNYDMTISVLK               +G
Sbjct: 550 LNLERLRMAIDDLLIKLAKTFSKAKSQTVFLINNYDMTISVLK--------------EAG 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
              G        +         EELLAEHFS LIKFVKTRGSEDLSSN DRPITVAEVEP
Sbjct: 610 PEGGKILVHFEDLLKSNTALFVEELLAEHFSQLIKFVKTRGSEDLSSNPDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRL+DCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLSDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. NCBI nr
Match: XP_008441201.1 (PREDICTED: vacuolar protein sorting-associated protein 52 A [Cucumis melo])

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 652/742 (87.87%), Postives = 667/742 (89.89%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           N SYD+AN+TQKNVFDLESFVGDLTVEEDACS+DISLEGLQQELEECKDD VV NILSKG
Sbjct: 10  NQSYDDANKTQKNVFDLESFVGDLTVEEDACSDDISLEGLQQELEECKDDDVVTNILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYI ESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYIKESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVD EVNDEYLRT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDGEVNDEYLRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           LEILSKKLVV EVDPMIKNSKALKDVQPELEKLRQKAVSKV+DF+VQKLHALRKPKTNIQ
Sbjct: 190 LEILSKKLVVAEVDPMIKNSKALKDVQPELEKLRQKAVSKVYDFLVQKLHALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVY EVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYSEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY
Sbjct: 310 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRSLHKLLMDTA+SE                              YNFCD+FFGE+S
Sbjct: 370 EVLFRSLHKLLMDTATSE------------------------------YNFCDDFFGEES 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MFY+IFAGPFAVIDE+F+SILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFYDIFAGPFAVIDEHFSSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRY EFTASLIHLNVEYGDGQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYAEFTASLIHLNVEYGDGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAK F+KAKSQTVFLINNYDMTISVLK      G     +EN  
Sbjct: 550 LNLERLRMAIDDLLIKLAKTFSKAKSQTVFLINNYDMTISVLKEAGPEGGKILVHFEN-- 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
                       +         EELLAEHFS LIKFVKTRGSEDLSSN DRPITVAEVEP
Sbjct: 610 ------------LLKSNTALFVEELLAEHFSQLIKFVKTRGSEDLSSNPDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRL+DCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLSDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. ExPASy Swiss-Prot
Match: Q94KD3 (Vacuolar protein sorting-associated protein 52 A OS=Arabidopsis thaliana OX=3702 GN=VPS52 PE=1 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 4.2e-306
Identity = 550/732 (75.14%), Postives = 615/732 (84.02%), Query Frame = 0

Query: 18  QKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKGVKLREYTKGV 77
           +K  FDL +FVGDL  EED+ SEDISLEGLQQELEEC+ D VVANILS G KLREY KGV
Sbjct: 20  EKLGFDLGAFVGDLAFEEDSGSEDISLEGLQQELEECESDEVVANILSSGDKLREYAKGV 79

Query: 78  ENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEIGSISADIKVL 137
           ENNLR+VELDSI++YI ESDNLVSLH+QIRDCD+ILSQMETLLSGFQ EIGSIS+DIK+L
Sbjct: 80  ENNLRKVELDSIEDYIKESDNLVSLHDQIRDCDSILSQMETLLSGFQEEIGSISSDIKIL 139

Query: 138 QEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRTLEILSKKLVV 197
           QEKSMDMGL+LKNR+VAESKLAKFVE+IIVPP+MID+IVD EVN+EY++TLEILSKKL  
Sbjct: 140 QEKSMDMGLRLKNRRVAESKLAKFVEDIIVPPKMIDVIVDGEVNEEYMKTLEILSKKLKF 199

Query: 198 VEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQILQQSVLLKY 257
           VE D  +K+SKALKDV+PELEKLRQKA+SKV+DFIVQKL ALRKPKTNIQILQQSVLLKY
Sbjct: 200 VEADQAVKSSKALKDVEPELEKLRQKAISKVYDFIVQKLIALRKPKTNIQILQQSVLLKY 259

Query: 258 KYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIATSSDLIGVEAR 317
           KY+ISFLK+H KEV+++VR AYIDTMNKVLSAHFRAYIQALEKLQLDIAT+ DLIGVE R
Sbjct: 260 KYIISFLKEHGKEVFMDVRAAYIDTMNKVLSAHFRAYIQALEKLQLDIATAYDLIGVETR 319

Query: 318 SSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPYEVLFRSLHKL 377
           ++GLF R REPLKNRSAVFALGDRIKI+K++D+PALIPHIAEASS+KYPYEVLFRSLHKL
Sbjct: 320 TTGLFSRAREPLKNRSAVFALGDRIKIIKDIDQPALIPHIAEASSLKYPYEVLFRSLHKL 379

Query: 378 LMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQSMFYEIFAGPF 437
           LMDTA+SEY                               FCD+FFGE+S+FYEIFAGPF
Sbjct: 380 LMDTATSEYM------------------------------FCDDFFGEESIFYEIFAGPF 439

Query: 438 AVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVNIALWPRFKMV 497
           +VIDE+FN +L N +DAIGLMLMI IIH HQLIMSRRRIPCLDSYLDKVNI+LWPRFKMV
Sbjct: 440 SVIDEHFNPVLSNCFDAIGLMLMIRIIHHHQLIMSRRRIPCLDSYLDKVNISLWPRFKMV 499

Query: 498 FDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLDLNLERLRMAI 557
           FD HLSSLR+AN+KTLWEDDVHPHYVMRRY EFTAS IHLNVEYGDGQLD+NLERLRMA+
Sbjct: 500 FDSHLSSLRDANIKTLWEDDVHPHYVMRRYAEFTASFIHLNVEYGDGQLDINLERLRMAV 559

Query: 558 DDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSGALRGSAEEQH 617
           D L++KLAK F + K Q VFLINNYDMTI+VLK               +G   G  +   
Sbjct: 560 DGLILKLAKLFPRPKQQIVFLINNYDMTIAVLK--------------EAGPEGGKIQMHF 619

Query: 618 SLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEPLVKDFASRWK 677
             +     +   EELL EHFS LIKFVK R SED S N +R IT+AEVEPLVKDF SRWK
Sbjct: 620 EEMLKSNTSLFVEELLVEHFSDLIKFVKNRASEDSSLNPERSITIAEVEPLVKDFGSRWK 679

Query: 678 AAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSALNKDLVSISS 737
            AIELM KD+ITSFSNFLCGM+ILRAALTQLLLYYTRLTDCIK+I GGSALN+DLVSI S
Sbjct: 680 TAIELMDKDIITSFSNFLCGMDILRAALTQLLLYYTRLTDCIKKIDGGSALNRDLVSIQS 707

Query: 738 IMYEIRKYSRTF 750
           IMYEIRKYS+TF
Sbjct: 740 IMYEIRKYSKTF 707

BLAST of Carg22869 vs. ExPASy Swiss-Prot
Match: Q9FVV6 (Vacuolar protein sorting-associated protein 52 B OS=Arabidopsis thaliana OX=3702 GN=P2 PE=2 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 1.5e-287
Identity = 518/745 (69.53%), Postives = 601/745 (80.67%), Query Frame = 0

Query: 12  DEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKGVKLR 71
           D+ +  +K+  +L +F GDL  EED+ SEDISLEGLQQELEEC+ D VVANILS G KLR
Sbjct: 2   DDFSNLEKHESELGAFFGDLAFEEDSRSEDISLEGLQQELEECESDEVVANILSSGDKLR 61

Query: 72  EYTKGVENNLRQVELDSIQ------EYITESDNLVSLHEQIRDCDNILSQMETLLSGFQA 131
           EY KGVENNLR+VELDS++      +YI ESD LVSLH+QIRDC+ ILSQMETLLSGFQ 
Sbjct: 62  EYAKGVENNLRKVELDSVEVAILLPDYIKESDKLVSLHDQIRDCNCILSQMETLLSGFQE 121

Query: 132 EIGSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYL 191
           EIGSIS+DIK+LQE SMDMGL+LKNR+V ESKLAKFVE+ IVPP+MI +IVD EVN+EY+
Sbjct: 122 EIGSISSDIKILQENSMDMGLRLKNRRVTESKLAKFVEDFIVPPKMIHVIVDGEVNEEYI 181

Query: 192 RTLEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTN 251
           +TL ILSKKL  VE D  +K+SKALKDV+PELEKLRQKA+SKV+DFIVQKL ALRKPKTN
Sbjct: 182 KTLGILSKKLKFVETDQAVKSSKALKDVEPELEKLRQKAISKVYDFIVQKLIALRKPKTN 241

Query: 252 IQILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDI 311
           IQILQQSV LKYKY+ISFLK+H KEV+++VR AYIDTMNKVLSAHF++YIQA EKLQLDI
Sbjct: 242 IQILQQSVFLKYKYIISFLKEHGKEVFMDVRAAYIDTMNKVLSAHFQSYIQAFEKLQLDI 301

Query: 312 ATSSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKY 371
           ATS+DLIGV+ RS+GLF R +EPLKNR AVFALG+RI+I+KE+D+PALIPHIAEASS+KY
Sbjct: 302 ATSNDLIGVDTRSTGLFSRSKEPLKNRCAVFALGERIQIIKEIDQPALIPHIAEASSLKY 361

Query: 372 PYEVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGE 431
           PYEVLFRSLHKLLMDTA+SEY                               FC++FFGE
Sbjct: 362 PYEVLFRSLHKLLMDTATSEYI------------------------------FCEDFFGE 421

Query: 432 QSMFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDK 491
           QS+FYEIFAGPF+VI E+ +S+L + YDAIGL+LMI IIH HQLIMSRRRIPCLDSYLDK
Sbjct: 422 QSIFYEIFAGPFSVIYEHLDSVLSSCYDAIGLLLMIRIIHHHQLIMSRRRIPCLDSYLDK 481

Query: 492 VNIALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQ 551
           VNI+LWPRFK VFD+H+ SLRNAN+ T+WEDDVHPHY+MRRY EFTAS IHLNVEYGDGQ
Sbjct: 482 VNISLWPRFKTVFDLHIGSLRNANINTIWEDDVHPHYIMRRYAEFTASFIHLNVEYGDGQ 541

Query: 552 LDLNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWEN 611
           LD+NLERLRMA+D L++KLAK F + K Q VFLINNYDMTI+VLK               
Sbjct: 542 LDINLERLRMAVDSLILKLAKLFPRPKQQMVFLINNYDMTIAVLK--------------- 601

Query: 612 SGALRGSAEEQHSLVCGKYNT-FMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAE 671
                G   + H     K NT    EELL EHFS +IKFVK+R +ED S N +R ITVAE
Sbjct: 602 EAEPEGGKIQMHFEELLKSNTSLFAEELLVEHFSDMIKFVKSRANEDSSPNLERSITVAE 661

Query: 672 VEPLVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVG 731
           VEPLVKDF SRWK AIELMHKD+IT FSNFLCGM+IL A +TQLLLYYTRL DCIK+I G
Sbjct: 662 VEPLVKDFGSRWKTAIELMHKDIITCFSNFLCGMDILIAGMTQLLLYYTRLEDCIKKIDG 701

Query: 732 GSALNKDLVSISSIMYEIRKYSRTF 750
           GSALN+D+V+  SIM+EI+KY +TF
Sbjct: 722 GSALNRDIVNYQSIMFEIKKYKKTF 701

BLAST of Carg22869 vs. ExPASy Swiss-Prot
Match: O55166 (Vacuolar protein sorting-associated protein 52 homolog OS=Rattus norvegicus OX=10116 GN=Vps52 PE=1 SV=2)

HSP 1 Score: 369.8 bits (948), Expect = 7.4e-101
Identity = 236/732 (32.24%), Postives = 398/732 (54.37%), Query Frame = 0

Query: 34  EEDACSEDISLEGLQQELEECKDDGVVANILSKGVKLREYTKGVENNLRQVELDSIQEYI 93
           E D  S++  L+ +   ++   +D +V   L  GV LR Y+K VE  L+Q+E  SI++YI
Sbjct: 44  ELDITSDEFILDEVDVHIQANLEDELVKEALKTGVDLRHYSKQVELELQQIEQKSIRDYI 103

Query: 94  TESDNLVSLHEQIRDCDNILSQMETLLSGFQAEIGSISADIKVLQEKSMDMGLKLKNRKV 153
            ES+N+ SLH QI  CD +L +ME +L  FQ+++ SIS +I+ LQE+S  M ++L+NR+ 
Sbjct: 104 QESENIASLHNQITACDAVLERMEQMLGAFQSDLSSISCEIRTLQEQSGAMNIRLRNRQA 163

Query: 154 AESKLAKFVEEIIVPPRMIDIIVDEEVND-EYLRTLEILSKKLVVVEVDPMIKNSKALKD 213
              KL + V+ ++VP  ++  I++  V +  +L  L+ L  K   V  +   + + A  D
Sbjct: 164 VRGKLGELVDGLVVPSALVTAILEAPVTEPRFLEQLQELDAKAAAVR-EQEARGTAACAD 223

Query: 214 VQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQILQQSVLLKYKYVISFLKDHSKEVY 273
           V+  L++LR KAV+K+ +FI+QK+++ RKP TN QI  Q+ LLKY++   FL  + +   
Sbjct: 224 VRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQI-PQTALLKYRFFYQFLLGNERATA 283

Query: 274 LEVRTAYIDTMNKVLSAHFRAYIQALEKLQL-DIATSSDLIGVEARSSGLFLRGREPLKN 333
            EVR  Y++T++K+  +++R+Y+  L K+Q  ++A   DL+GVE  +   F   +  L++
Sbjct: 284 KEVRDEYVETLSKIYLSYYRSYVGRLMKVQYEEVAEKDDLMGVEDTAKKGFF-SKPSLRS 343

Query: 334 RSAVFALGDRIKILK--EVDEPALIPHIAEASSIKYPYEVLFRSLHKLLMDTASSEYFAF 393
           R+ +F LG R  ++   E++ P L+PH A+    +YP+E LFRS H  L+D         
Sbjct: 344 RNTIFTLGTRGAVISPAELEAPILVPHTAQRGEQRYPFEALFRSQHYALLD--------- 403

Query: 394 YSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFF-----GEQSMFYEIFAGPFAVIDEYF 453
                                NS   Y F  EFF         +F+ +     ++  ++ 
Sbjct: 404 ---------------------NSCREYLFICEFFVVSGPAAHDLFHAVMGRTLSMTLKHL 463

Query: 454 NSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVNIALWPRFKMVFDMHLSS 513
            S L + YDAI + L I I+ + + I ++R +P LD Y ++V   LWPRF+++ +M++ S
Sbjct: 464 ESYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQS 523

Query: 514 LRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLDLNLERLRMAIDDLLIKL 573
           +R+ + + L   D  PHY+ RRY EF+++L+ +N    + +    L +L++ +++ ++++
Sbjct: 524 VRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTLQLLGQLQVEVENFVLRV 583

Query: 574 AKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSGALRGSAEEQHSLVCGKY 633
           A  F+  K Q VFLINNYDM + VL              E +       E    L+  + 
Sbjct: 584 AAEFSSRKEQLVFLINNYDMMLGVL-------------MERAADDSKEVESFQQLLNART 643

Query: 634 NTFMQEELLAEHFSSLIKFVK------TRGSEDLSSNSDRPITVAEVEPLVKDFASRWKA 693
             F+ EELL+  F  L+ FVK       RG  +     +     A V  L++ F S WKA
Sbjct: 644 QEFI-EELLSPPFGGLVAFVKEAEALIERGQAERLRGEE-----ARVTQLIRGFGSSWKA 703

Query: 694 AIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKR-IVGGSALNKDLVSISS 750
           ++E + +DV+ SF+NF  G  I++ ALTQL+  Y R    + +  +       +L++I  
Sbjct: 704 SVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRALPARAELINIHH 723

BLAST of Carg22869 vs. ExPASy Swiss-Prot
Match: Q5TJF0 (Vacuolar protein sorting-associated protein 52 homolog OS=Canis lupus familiaris OX=9615 GN=VPS52 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 9.7e-101
Identity = 235/727 (32.32%), Postives = 398/727 (54.75%), Query Frame = 0

Query: 34  EEDACSEDISLEGLQQELEECKDDGVVANILSKGVKLREYTKGVENNLRQVELDSIQEYI 93
           E D  S++  L+ +   ++   +D +V   L  GV LR Y+K VE  L+Q+E  SI++YI
Sbjct: 44  ELDISSDEFILDEVDVHIQANLEDELVKEALKTGVDLRHYSKQVELELQQIEQKSIRDYI 103

Query: 94  TESDNLVSLHEQIRDCDNILSQMETLLSGFQAEIGSISADIKVLQEKSMDMGLKLKNRKV 153
            ES+N+ SLH QI  CD +L +ME +L  FQ+++ SIS++I+ LQE+S  M ++L+NR+ 
Sbjct: 104 QESENIASLHNQITACDAVLERMEQMLGAFQSDLSSISSEIRTLQEQSGAMNIRLRNRQA 163

Query: 154 AESKLAKFVEEIIVPPRMIDIIVDEEVND-EYLRTLEILSKKLVVVEVDPMIKNSKALKD 213
              KL + V+ +IVP  +I  I++  V +  +L  L+ L  K   V  +   + + A  D
Sbjct: 164 VRGKLGELVDGLIVPSALIMAILEAPVTEPRFLEQLQELDAKAAAVR-EQEARGTAACAD 223

Query: 214 VQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQILQQSVLLKYKYVISFLKDHSKEVY 273
           V+  L++LR KAV+K+ +FI+QK+++ RKP TN QI  Q+ LLKY++   FL  + +   
Sbjct: 224 VRGILDRLRVKAVTKIREFILQKIYSFRKPMTNYQI-PQTALLKYRFFYQFLLGNERATA 283

Query: 274 LEVRTAYIDTMNKVLSAHFRAYIQALEKLQL-DIATSSDLIGVEARSSGLFLRGREPLKN 333
            E+R  Y++T++K+  +++R+Y+  L K+Q  ++A   DL+GVE  +   F   +  L++
Sbjct: 284 KEIRDEYVETLSKIYLSYYRSYLGRLMKVQYEEVAEKDDLMGVEDTAKKGFF-SKPSLRS 343

Query: 334 RSAVFALGDRIKILK--EVDEPALIPHIAEASSIKYPYEVLFRSLHKLLMDTASSEYFAF 393
           R+ +F LG R  ++   E++ P L+PH A+    +YP+E LFRS H  L+D +  EY   
Sbjct: 344 RNTIFTLGTRGSVISPTELEAPILVPHTAQRGEQRYPFEALFRSQHYALLDNSCREYL-- 403

Query: 394 YSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQSMFYEIFAGPFAVIDEYFNSILP 453
                      IC  FV                     +F+ +      +  ++  S L 
Sbjct: 404 ----------FICEFFV-------------VSGSAAHDLFHAVMGRTLGMTLKHLESYLT 463

Query: 454 NSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVNIALWPRFKMVFDMHLSSLRNAN 513
           + YDAI + L I I+ + + I ++R +P LD Y ++V   LWPRF+++ +M++ S+R+ +
Sbjct: 464 DCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQSVRSTD 523

Query: 514 VKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLDLNLERLRMAIDDLLIKLAKAFT 573
            + L   D  PHY+ RRY EF+++L+ +N    + +    L +L++ +++ ++++A  F+
Sbjct: 524 PQRLGGLDTRPHYITRRYAEFSSALVSINQTVPNERTMQLLGQLQVEVENFVLRVAAEFS 583

Query: 574 KAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSGALRGSAEEQHSLVCGKYNTFMQ 633
             K Q VFLINNYDM + VL              E +       E    L+  +   F+ 
Sbjct: 584 SRKEQLVFLINNYDMMLGVL-------------MERAADDSKEVESFQQLLNARTQEFI- 643

Query: 634 EELLAEHFSSLIKFVK------TRGSEDLSSNSDRPITVAEVEPLVKDFASRWKAAIELM 693
           EELL+  F  L+ FVK       RG  +     +     A V  L++ F S WK+++E +
Sbjct: 644 EELLSPPFGGLVAFVKEAEALIERGQAERLRGEE-----ARVTQLIRGFGSSWKSSVESL 703

Query: 694 HKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKR-IVGGSALNKDLVSISSIMYEI 750
            +DV+ SF+NF  G  I++ ALTQL+  Y R    + +  +       +L++I  +M E+
Sbjct: 704 SQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRALPARAELINIHHLMVEL 723

BLAST of Carg22869 vs. ExPASy Swiss-Prot
Match: Q8N1B4 (Vacuolar protein sorting-associated protein 52 homolog OS=Homo sapiens OX=9606 GN=VPS52 PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 9.7e-101
Identity = 234/732 (31.97%), Postives = 400/732 (54.64%), Query Frame = 0

Query: 34  EEDACSEDISLEGLQQELEECKDDGVVANILSKGVKLREYTKGVENNLRQVELDSIQEYI 93
           E D  S++  L+ +   ++   +D +V   L  GV LR Y+K VE  L+Q+E  SI++YI
Sbjct: 44  ELDITSDEFILDEVDVHIQANLEDELVKEALKTGVDLRHYSKQVELELQQIEQKSIRDYI 103

Query: 94  TESDNLVSLHEQIRDCDNILSQMETLLSGFQAEIGSISADIKVLQEKSMDMGLKLKNRKV 153
            ES+N+ SLH QI  CD +L +ME +L  FQ+++ SIS++I+ LQE+S  M ++L+NR+ 
Sbjct: 104 QESENIASLHNQITACDAVLERMEQMLGAFQSDLSSISSEIRTLQEQSGAMNIRLRNRQA 163

Query: 154 AESKLAKFVEEIIVPPRMIDIIVDEEVND-EYLRTLEILSKKLVVVEVDPMIKNSKALKD 213
              KL + V+ ++VP  ++  I++  V +  +L  L+ L  K   V  +   + + A  D
Sbjct: 164 VRGKLGELVDGLVVPSALVTAILEAPVTEPRFLEQLQELDAKAAAVR-EQEARGTAACAD 223

Query: 214 VQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQILQQSVLLKYKYVISFLKDHSKEVY 273
           V+  L++LR KAV+K+ +FI+QK+++ RKP TN QI  Q+ LLKY++   FL  + +   
Sbjct: 224 VRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQI-PQTALLKYRFFYQFLLGNERATA 283

Query: 274 LEVRTAYIDTMNKVLSAHFRAYIQALEKLQL-DIATSSDLIGVEARSSGLFLRGREPLKN 333
            E+R  Y++T++K+  +++R+Y+  L K+Q  ++A   DL+GVE  +   F   +  L++
Sbjct: 284 KEIRDEYVETLSKIYLSYYRSYLGRLMKVQYEEVAEKDDLMGVEDTAKKGFF-SKPSLRS 343

Query: 334 RSAVFALGDRIKILK--EVDEPALIPHIAEASSIKYPYEVLFRSLHKLLMDTASSEYFAF 393
           R+ +F LG R  ++   E++ P L+PH A+    +YP+E LFRS H  L+D         
Sbjct: 344 RNTIFTLGTRGSVISPTELEAPILVPHTAQRGEQRYPFEALFRSQHYALLD--------- 403

Query: 394 YSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFF-----GEQSMFYEIFAGPFAVIDEYF 453
                                NS   Y F  EFF         +F+ +     ++  ++ 
Sbjct: 404 ---------------------NSCREYLFICEFFVVSGPAAHDLFHAVMGRTLSMTLKHL 463

Query: 454 NSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVNIALWPRFKMVFDMHLSS 513
           +S L + YDAI + L I I+ + + I ++R +P LD Y ++V   LWPRF+++ +M++ S
Sbjct: 464 DSYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQS 523

Query: 514 LRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLDLNLERLRMAIDDLLIKL 573
           +R+ + + L   D  PHY+ RRY EF+++L+ +N    + +    L +L++ +++ ++++
Sbjct: 524 VRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTMQLLGQLQVEVENFVLRV 583

Query: 574 AKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSGALRGSAEEQHSLVCGKY 633
           A  F+  K Q VFLINNYDM + VL              E +       E    L+  + 
Sbjct: 584 AAEFSSRKEQLVFLINNYDMMLGVL-------------MERAADDSKEVESFQQLLNART 643

Query: 634 NTFMQEELLAEHFSSLIKFVK------TRGSEDLSSNSDRPITVAEVEPLVKDFASRWKA 693
             F+ EELL+  F  L+ FVK       RG  +     +     A V  L++ F S WK+
Sbjct: 644 QEFI-EELLSPPFGGLVAFVKEAEALIERGQAERLRGEE-----ARVTQLIRGFGSSWKS 703

Query: 694 AIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKR-IVGGSALNKDLVSISS 750
           ++E + +DV+ SF+NF  G  I++ ALTQL+  Y R    + +  +       +L++I  
Sbjct: 704 SVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRALPARAELINIHH 723

BLAST of Carg22869 vs. ExPASy TrEMBL
Match: A0A6J1FFB1 (vacuolar protein sorting-associated protein 52 A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444932 PE=3 SV=1)

HSP 1 Score: 1259.2 bits (3257), Expect = 0.0e+00
Identity = 675/742 (90.97%), Postives = 677/742 (91.24%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG
Sbjct: 10  NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ
Sbjct: 190 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY
Sbjct: 310 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRSLHKLLMDTASSE                              YNFCDEFFGEQS
Sbjct: 370 EVLFRSLHKLLMDTASSE------------------------------YNFCDEFFGEQS 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLK               +G
Sbjct: 550 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLK--------------EAG 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
              G        +         EELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP
Sbjct: 610 PEGGKILVHFEDLLKSNTALFVEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. ExPASy TrEMBL
Match: A0A6J1K443 (vacuolar protein sorting-associated protein 52 A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489872 PE=3 SV=1)

HSP 1 Score: 1257.7 bits (3253), Expect = 0.0e+00
Identity = 674/742 (90.84%), Postives = 677/742 (91.24%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG
Sbjct: 10  NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           L+ILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ
Sbjct: 190 LKILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY
Sbjct: 310 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRSLHKLLMDTASSE                              YNFCDEFFGEQS
Sbjct: 370 EVLFRSLHKLLMDTASSE------------------------------YNFCDEFFGEQS 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLK               +G
Sbjct: 550 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLK--------------EAG 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
              G        +         EELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP
Sbjct: 610 PEGGKILVHFEDLLKSNTALFVEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. ExPASy TrEMBL
Match: A0A1S3B3M6 (vacuolar protein sorting-associated protein 52 A OS=Cucumis melo OX=3656 GN=LOC103485406 PE=3 SV=1)

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 652/742 (87.87%), Postives = 667/742 (89.89%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           N SYD+AN+TQKNVFDLESFVGDLTVEEDACS+DISLEGLQQELEECKDD VV NILSKG
Sbjct: 10  NQSYDDANKTQKNVFDLESFVGDLTVEEDACSDDISLEGLQQELEECKDDDVVTNILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYI ESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYIKESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVD EVNDEYLRT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDGEVNDEYLRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           LEILSKKLVV EVDPMIKNSKALKDVQPELEKLRQKAVSKV+DF+VQKLHALRKPKTNIQ
Sbjct: 190 LEILSKKLVVAEVDPMIKNSKALKDVQPELEKLRQKAVSKVYDFLVQKLHALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVY EVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYSEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY
Sbjct: 310 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRSLHKLLMDTA+SE                              YNFCD+FFGE+S
Sbjct: 370 EVLFRSLHKLLMDTATSE------------------------------YNFCDDFFGEES 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MFY+IFAGPFAVIDE+F+SILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFYDIFAGPFAVIDEHFSSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRY EFTASLIHLNVEYGDGQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYAEFTASLIHLNVEYGDGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAK F+KAKSQTVFLINNYDMTISVLK      G     +EN  
Sbjct: 550 LNLERLRMAIDDLLIKLAKTFSKAKSQTVFLINNYDMTISVLKEAGPEGGKILVHFEN-- 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
                       +         EELLAEHFS LIKFVKTRGSEDLSSN DRPITVAEVEP
Sbjct: 610 ------------LLKSNTALFVEELLAEHFSQLIKFVKTRGSEDLSSNPDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRL+DCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLSDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. ExPASy TrEMBL
Match: A0A6J1DHR6 (vacuolar protein sorting-associated protein 52 A OS=Momordica charantia OX=3673 GN=LOC111020176 PE=3 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 647/742 (87.20%), Postives = 666/742 (89.76%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           +HSYDEAN+T KNVFDLESFVGDLTVEEDACS+DISLEGLQQEL+ECKDD VV+NILSKG
Sbjct: 10  SHSYDEANKTPKNVFDLESFVGDLTVEEDACSDDISLEGLQQELQECKDDDVVSNILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYI ESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYIKESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVD EVNDEY+RT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDGEVNDEYMRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           LEILSKKLVVVE DPMIK+SKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ
Sbjct: 190 LEILSKKLVVVEADPMIKHSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVY EVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYNEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKE+DEPALIPHIAEASSIKYPY
Sbjct: 310 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEIDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRSLHKLLMDTASSE                              YNFCD+FFGE+S
Sbjct: 370 EVLFRSLHKLLMDTASSE------------------------------YNFCDDFFGEES 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MF++IFAGPFAVIDE+FNSILPN YDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFHDIFAGPFAVIDEHFNSILPNCYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANV+TLWEDDVHPHYVMRRY EFTASLIHLNVEYG+GQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVRTLWEDDVHPHYVMRRYAEFTASLIHLNVEYGEGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAK F+KAKSQTVFLINNYDMTISVLK               +G
Sbjct: 550 LNLERLRMAIDDLLIKLAKTFSKAKSQTVFLINNYDMTISVLK--------------EAG 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
              G        +         EELLAEHFS LIKFVKTRGSEDLSSN DRPITVAEVEP
Sbjct: 610 PEGGKILVHFEDLLKSNTALFVEELLAEHFSELIKFVKTRGSEDLSSNPDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRL+DCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLSDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. ExPASy TrEMBL
Match: A0A0A0LSV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G402000 PE=3 SV=1)

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 648/742 (87.33%), Postives = 662/742 (89.22%), Query Frame = 0

Query: 8   NHSYDEANQTQKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKG 67
           N SYDEAN+T KNVFDLESFVGDLTVEEDACS+DISLEGLQQELEECKDD VV NILSKG
Sbjct: 10  NQSYDEANKTPKNVFDLESFVGDLTVEEDACSDDISLEGLQQELEECKDDDVVTNILSKG 69

Query: 68  VKLREYTKGVENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 127
           VKLREYTKGVENNLRQVELDSIQEYI ESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI
Sbjct: 70  VKLREYTKGVENNLRQVELDSIQEYIKESDNLVSLHEQIRDCDNILSQMETLLSGFQAEI 129

Query: 128 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRT 187
           GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVD EVNDEYLRT
Sbjct: 130 GSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDGEVNDEYLRT 189

Query: 188 LEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQ 247
           LEILSKKLVV EVDPMIKNSKALKDVQPELEKLRQKAVSKV+DF+VQKL ALRKPKTNIQ
Sbjct: 190 LEILSKKLVVAEVDPMIKNSKALKDVQPELEKLRQKAVSKVYDFLVQKLQALRKPKTNIQ 249

Query: 248 ILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 307
           ILQQSVLLKYKYVISFLKDHSKEVY EVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT
Sbjct: 250 ILQQSVLLKYKYVISFLKDHSKEVYNEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIAT 309

Query: 308 SSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 367
           S+DLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY
Sbjct: 310 SNDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPY 369

Query: 368 EVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQS 427
           EVLFRSLHKLLMDTA+SE                              YNFCD+FFGE+ 
Sbjct: 370 EVLFRSLHKLLMDTATSE------------------------------YNFCDDFFGEEP 429

Query: 428 MFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 487
           MFY+IFAGPFAVIDE+F SILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN
Sbjct: 430 MFYDIFAGPFAVIDEHFTSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVN 489

Query: 488 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLD 547
           IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRY EFTASLIHLNVEYGDGQLD
Sbjct: 490 IALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMRRYAEFTASLIHLNVEYGDGQLD 549

Query: 548 LNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSG 607
           LNLERLRMAIDDLLIKLAK F+KAKSQTVFLINNYDMTISVLK               +G
Sbjct: 550 LNLERLRMAIDDLLIKLAKTFSKAKSQTVFLINNYDMTISVLK--------------EAG 609

Query: 608 ALRGSAEEQHSLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEP 667
              G        +         EELLAEHFS LIKFVKTRGSEDLSSN DRPITVAEVEP
Sbjct: 610 PEGGKILVHFEDLLKSNTALFVEELLAEHFSQLIKFVKTRGSEDLSSNPDRPITVAEVEP 669

Query: 668 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSA 727
           LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRL+DCIKRIVGGSA
Sbjct: 670 LVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLSDCIKRIVGGSA 707

Query: 728 LNKDLVSISSIMYEIRKYSRTF 750
           LNKDLVSISSIMYEIRKYSRTF
Sbjct: 730 LNKDLVSISSIMYEIRKYSRTF 707

BLAST of Carg22869 vs. TAIR 10
Match: AT1G71270.1 (Vps52 / Sac2 family )

HSP 1 Score: 1051.6 bits (2718), Expect = 3.0e-307
Identity = 550/732 (75.14%), Postives = 615/732 (84.02%), Query Frame = 0

Query: 18  QKNVFDLESFVGDLTVEEDACSEDISLEGLQQELEECKDDGVVANILSKGVKLREYTKGV 77
           +K  FDL +FVGDL  EED+ SEDISLEGLQQELEEC+ D VVANILS G KLREY KGV
Sbjct: 20  EKLGFDLGAFVGDLAFEEDSGSEDISLEGLQQELEECESDEVVANILSSGDKLREYAKGV 79

Query: 78  ENNLRQVELDSIQEYITESDNLVSLHEQIRDCDNILSQMETLLSGFQAEIGSISADIKVL 137
           ENNLR+VELDSI++YI ESDNLVSLH+QIRDCD+ILSQMETLLSGFQ EIGSIS+DIK+L
Sbjct: 80  ENNLRKVELDSIEDYIKESDNLVSLHDQIRDCDSILSQMETLLSGFQEEIGSISSDIKIL 139

Query: 138 QEKSMDMGLKLKNRKVAESKLAKFVEEIIVPPRMIDIIVDEEVNDEYLRTLEILSKKLVV 197
           QEKSMDMGL+LKNR+VAESKLAKFVE+IIVPP+MID+IVD EVN+EY++TLEILSKKL  
Sbjct: 140 QEKSMDMGLRLKNRRVAESKLAKFVEDIIVPPKMIDVIVDGEVNEEYMKTLEILSKKLKF 199

Query: 198 VEVDPMIKNSKALKDVQPELEKLRQKAVSKVFDFIVQKLHALRKPKTNIQILQQSVLLKY 257
           VE D  +K+SKALKDV+PELEKLRQKA+SKV+DFIVQKL ALRKPKTNIQILQQSVLLKY
Sbjct: 200 VEADQAVKSSKALKDVEPELEKLRQKAISKVYDFIVQKLIALRKPKTNIQILQQSVLLKY 259

Query: 258 KYVISFLKDHSKEVYLEVRTAYIDTMNKVLSAHFRAYIQALEKLQLDIATSSDLIGVEAR 317
           KY+ISFLK+H KEV+++VR AYIDTMNKVLSAHFRAYIQALEKLQLDIAT+ DLIGVE R
Sbjct: 260 KYIISFLKEHGKEVFMDVRAAYIDTMNKVLSAHFRAYIQALEKLQLDIATAYDLIGVETR 319

Query: 318 SSGLFLRGREPLKNRSAVFALGDRIKILKEVDEPALIPHIAEASSIKYPYEVLFRSLHKL 377
           ++GLF R REPLKNRSAVFALGDRIKI+K++D+PALIPHIAEASS+KYPYEVLFRSLHKL
Sbjct: 320 TTGLFSRAREPLKNRSAVFALGDRIKIIKDIDQPALIPHIAEASSLKYPYEVLFRSLHKL 379

Query: 378 LMDTASSEYFAFYSSFVYYLQNVICSKFVNFLINSFHRYNFCDEFFGEQSMFYEIFAGPF 437
           LMDTA+SEY                               FCD+FFGE+S+FYEIFAGPF
Sbjct: 380 LMDTATSEYM------------------------------FCDDFFGEESIFYEIFAGPF 439

Query: 438 AVIDEYFNSILPNSYDAIGLMLMILIIHQHQLIMSRRRIPCLDSYLDKVNIALWPRFKMV 497
           +VIDE+FN +L N +DAIGLMLMI IIH HQLIMSRRRIPCLDSYLDKVNI+LWPRFKMV
Sbjct: 440 SVIDEHFNPVLSNCFDAIGLMLMIRIIHHHQLIMSRRRIPCLDSYLDKVNISLWPRFKMV 499

Query: 498 FDMHLSSLRNANVKTLWEDDVHPHYVMRRYVEFTASLIHLNVEYGDGQLDLNLERLRMAI 557
           FD HLSSLR+AN+KTLWEDDVHPHYVMRRY EFTAS IHLNVEYGDGQLD+NLERLRMA+
Sbjct: 500 FDSHLSSLRDANIKTLWEDDVHPHYVMRRYAEFTASFIHLNVEYGDGQLDINLERLRMAV 559

Query: 558 DDLLIKLAKAFTKAKSQTVFLINNYDMTISVLKVGFGSQGSWSGGWENSGALRGSAEEQH 617
           D L++KLAK F + K Q VFLINNYDMTI+VLK               +G   G  +   
Sbjct: 560 DGLILKLAKLFPRPKQQIVFLINNYDMTIAVLK--------------EAGPEGGKIQMHF 619

Query: 618 SLVCGKYNTFMQEELLAEHFSSLIKFVKTRGSEDLSSNSDRPITVAEVEPLVKDFASRWK 677
             +     +   EELL EHFS LIKFVK R SED S N +R IT+AEVEPLVKDF SRWK
Sbjct: 620 EEMLKSNTSLFVEELLVEHFSDLIKFVKNRASEDSSLNPERSITIAEVEPLVKDFGSRWK 679

Query: 678 AAIELMHKDVITSFSNFLCGMEILRAALTQLLLYYTRLTDCIKRIVGGSALNKDLVSISS 737
            AIELM KD+ITSFSNFLCGM+ILRAALTQLLLYYTRLTDCIK+I GGSALN+DLVSI S
Sbjct: 680 TAIELMDKDIITSFSNFLCGMDILRAALTQLLLYYTRLTDCIKKIDGGSALNRDLVSIQS 707

Query: 738 IMYEIRKYSRTF 750
           IMYEIRKYS+TF
Sbjct: 740 IMYEIRKYSKTF 707

BLAST of Carg22869 vs. TAIR 10
Match: AT1G71300.1 (Vps52 / Sac2 family )

HSP 1 Score: 978.0 bits (2527), Expect = 4.2e-285
Identity = 518/765 (67.71%), Postives = 601/765 (78.56%), Query Frame = 0

Query: 12  DEANQTQKNVFDLESFVGDLTVEEDA--------------------CSEDISLEGLQQEL 71
           D+ +  +K+  +L +F GDL  EED+                     SEDISLEGLQQEL
Sbjct: 2   DDFSNLEKHESELGAFFGDLAFEEDSRRMLSFGSVSFIKSTLLFLLGSEDISLEGLQQEL 61

Query: 72  EECKDDGVVANILSKGVKLREYTKGVENNLRQVELDSIQ------EYITESDNLVSLHEQ 131
           EEC+ D VVANILS G KLREY KGVENNLR+VELDS++      +YI ESD LVSLH+Q
Sbjct: 62  EECESDEVVANILSSGDKLREYAKGVENNLRKVELDSVEVAILLPDYIKESDKLVSLHDQ 121

Query: 132 IRDCDNILSQMETLLSGFQAEIGSISADIKVLQEKSMDMGLKLKNRKVAESKLAKFVEEI 191
           IRDC+ ILSQMETLLSGFQ EIGSIS+DIK+LQE SMDMGL+LKNR+V ESKLAKFVE+ 
Sbjct: 122 IRDCNCILSQMETLLSGFQEEIGSISSDIKILQENSMDMGLRLKNRRVTESKLAKFVEDF 181

Query: 192 IVPPRMIDIIVDEEVNDEYLRTLEILSKKLVVVEVDPMIKNSKALKDVQPELEKLRQKAV 251
           IVPP+MI +IVD EVN+EY++TL ILSKKL  VE D  +K+SKALKDV+PELEKLRQKA+
Sbjct: 182 IVPPKMIHVIVDGEVNEEYIKTLGILSKKLKFVETDQAVKSSKALKDVEPELEKLRQKAI 241

Query: 252 SKVFDFIVQKLHALRKPKTNIQILQQSVLLKYKYVISFLKDHSKEVYLEVRTAYIDTMNK 311
           SKV+DFIVQKL ALRKPKTNIQILQQSV LKYKY+ISFLK+H KEV+++VR AYIDTMNK
Sbjct: 242 SKVYDFIVQKLIALRKPKTNIQILQQSVFLKYKYIISFLKEHGKEVFMDVRAAYIDTMNK 301

Query: 312 VLSAHFRAYIQALEKLQLDIATSSDLIGVEARSSGLFLRGREPLKNRSAVFALGDRIKIL 371
           VLSAHF++YIQA EKLQLDIATS+DLIGV+ RS+GLF R +EPLKNR AVFALG+RI+I+
Sbjct: 302 VLSAHFQSYIQAFEKLQLDIATSNDLIGVDTRSTGLFSRSKEPLKNRCAVFALGERIQII 361

Query: 372 KEVDEPALIPHIAEASSIKYPYEVLFRSLHKLLMDTASSEYFAFYSSFVYYLQNVICSKF 431
           KE+D+PALIPHIAEASS+KYPYEVLFRSLHKLLMDTA+SEY                   
Sbjct: 362 KEIDQPALIPHIAEASSLKYPYEVLFRSLHKLLMDTATSEYI------------------ 421

Query: 432 VNFLINSFHRYNFCDEFFGEQSMFYEIFAGPFAVIDEYFNSILPNSYDAIGLMLMILIIH 491
                       FC++FFGEQS+FYEIFAGPF+VI E+ +S+L + YDAIGL+LMI IIH
Sbjct: 422 ------------FCEDFFGEQSIFYEIFAGPFSVIYEHLDSVLSSCYDAIGLLLMIRIIH 481

Query: 492 QHQLIMSRRRIPCLDSYLDKVNIALWPRFKMVFDMHLSSLRNANVKTLWEDDVHPHYVMR 551
            HQLIMSRRRIPCLDSYLDKVNI+LWPRFK VFD+H+ SLRNAN+ T+WEDDVHPHY+MR
Sbjct: 482 HHQLIMSRRRIPCLDSYLDKVNISLWPRFKTVFDLHIGSLRNANINTIWEDDVHPHYIMR 541

Query: 552 RYVEFTASLIHLNVEYGDGQLDLNLERLRMAIDDLLIKLAKAFTKAKSQTVFLINNYDMT 611
           RY EFTAS IHLNVEYGDGQLD+NLERLRMA+D L++KLAK F + K Q VFLINNYDMT
Sbjct: 542 RYAEFTASFIHLNVEYGDGQLDINLERLRMAVDSLILKLAKLFPRPKQQMVFLINNYDMT 601

Query: 612 ISVLKVGFGSQGSWSGGWENSGALRGSAEEQHSLVCGKYNT-FMQEELLAEHFSSLIKFV 671
           I+VLK                    G   + H     K NT    EELL EHFS +IKFV
Sbjct: 602 IAVLK---------------EAEPEGGKIQMHFEELLKSNTSLFAEELLVEHFSDMIKFV 661

Query: 672 KTRGSEDLSSNSDRPITVAEVEPLVKDFASRWKAAIELMHKDVITSFSNFLCGMEILRAA 731
           K+R +ED S N +R ITVAEVEPLVKDF SRWK AIELMHKD+IT FSNFLCGM+IL A 
Sbjct: 662 KSRANEDSSPNLERSITVAEVEPLVKDFGSRWKTAIELMHKDIITCFSNFLCGMDILIAG 721

Query: 732 LTQLLLYYTRLTDCIKRIVGGSALNKDLVSISSIMYEIRKYSRTF 750
           +TQLLLYYTRL DCIK+I GGSALN+D+V+  SIM+EI+KY +TF
Sbjct: 722 MTQLLLYYTRLEDCIKKIDGGSALNRDIVNYQSIMFEIKKYKKTF 721

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015901.10.0e+00100.00Vacuolar protein sorting-associated protein 52 A [Cucurbita argyrosperma subsp. ... [more]
XP_022938844.10.0e+0090.97vacuolar protein sorting-associated protein 52 A isoform X1 [Cucurbita moschata]... [more]
XP_022994068.10.0e+0090.84vacuolar protein sorting-associated protein 52 A-like isoform X1 [Cucurbita maxi... [more]
XP_038884385.10.0e+0087.87vacuolar protein sorting-associated protein 52 A isoform X1 [Benincasa hispida][more]
XP_008441201.10.0e+0087.87PREDICTED: vacuolar protein sorting-associated protein 52 A [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q94KD34.2e-30675.14Vacuolar protein sorting-associated protein 52 A OS=Arabidopsis thaliana OX=3702... [more]
Q9FVV61.5e-28769.53Vacuolar protein sorting-associated protein 52 B OS=Arabidopsis thaliana OX=3702... [more]
O551667.4e-10132.24Vacuolar protein sorting-associated protein 52 homolog OS=Rattus norvegicus OX=1... [more]
Q5TJF09.7e-10132.32Vacuolar protein sorting-associated protein 52 homolog OS=Canis lupus familiaris... [more]
Q8N1B49.7e-10131.97Vacuolar protein sorting-associated protein 52 homolog OS=Homo sapiens OX=9606 G... [more]
Match NameE-valueIdentityDescription
A0A6J1FFB10.0e+0090.97vacuolar protein sorting-associated protein 52 A isoform X1 OS=Cucurbita moschat... [more]
A0A6J1K4430.0e+0090.84vacuolar protein sorting-associated protein 52 A-like isoform X1 OS=Cucurbita ma... [more]
A0A1S3B3M60.0e+0087.87vacuolar protein sorting-associated protein 52 A OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1DHR60.0e+0087.20vacuolar protein sorting-associated protein 52 A OS=Momordica charantia OX=3673 ... [more]
A0A0A0LSV20.0e+0087.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G402000 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G71270.13.0e-30775.14Vps52 / Sac2 family [more]
AT1G71300.14.2e-28567.71Vps52 / Sac2 family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007258Vps52PFAMPF04129Vps52coord: 86..392
e-value: 3.0E-111
score: 372.7
coord: 395..590
e-value: 8.9E-71
score: 239.1
IPR007258Vps52PANTHERPTHR14190SUPPRESSOR OF ACTIN MUTATIONS 2/VACUOLAR PROTEIN SORTING 52coord: 26..746

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22869-RACarg22869-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032456 endocytic recycling
biological_process GO:0006896 Golgi to vacuole transport
biological_process GO:0015031 protein transport
biological_process GO:0042147 retrograde transport, endosome to Golgi
cellular_component GO:0005829 cytosol
cellular_component GO:0000938 GARP complex
molecular_function GO:0019905 syntaxin binding