Carg22851 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22851
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionalkylated DNA repair protein alkB homolog 8
LocationCarg_Chr15: 369269 .. 372542 (-)
RNA-Seq ExpressionCarg22851
SyntenyCarg22851
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAACTAAAAATTTTGACAACTCTGCAAATGAATGTACAGTGAAGCTAGGGCACTCTTCCCCCTTCACTTCGCGACGAAGACGACCAGCCATTTGAAGCTGCCATTGCGAGATTAGTGCCTTGATTTTCTCTTATGATTCTTCTTCTTCTTTCTTTTTCCAATCATGAACTAGATTCCACAATCTCGACCTTCCATTTGATTCTTTCTAATCTTTCAGCACAATCAGGCAGCCGCGCCGTACCTGAGTTTCGTGAGTTTTGTTTCTATCTCGCTTTCTAGATCTATTTATATCACGGCTGCTATTTTGTAGTTTGCACGATGTTTTCTCTACTTACTACTGGTTCTGTGTTAGTTTCTTACATTTCATTCAATCGCATGTACGTTTTTGGTTCTTATCTTGCTGTAGGATAATGGATATTGTATCTCCCCTTTTCTTCCCCTTGGAAATCTGTGAAGGTTCAATAATGAATGTGATGATCCTTGCTCTGATAATACGGCCAAGTTGCTACATTAATGCCAACCCACTTCATTATTGCGTTGTCCTTTTCCCAATCCTCCGATGTCTTCTATTTCTGTCTGTCCATATTTTCTACAAAAAGTAGTTGACTAAACCTGTCTAAATGAGTTTGGCTTTCCTTCTGGATGATTACCTCGCTAAAATGTAAAGAATAAAATCCTTGATATTAGAGGGAGCTATTGGTGAGATTGAAGGAACTCATAAGTGCCAGCCTGACTCCTTGTATATTAACAGTGAATGATTAAACAGTCTATCTTATATCAGCCCTGCAAGCTACATATCCCTTTCGATCGAGCAGCATGCTCGGCTATTATTCTGTAATCTGTCAGAACATGGAATTTCGCGGGGTAACTGAATCCTACGGAAGAGTTGTATTTTTTTTTAAGATCTCGCAGTTCCTAGTGATCTTCGAAAGAGTTGCAGGGAGGGTCCACTGTTACATATCGTGTCACTGAAAGGATTTGATGGAGAAAACTTCTGAGGTTTCAAGCCTCTAGTGTCTAATTTTACGATTTGAATGGCAGTCATTGGGCCCATGCAGCACTCAATTGAACGTAAATAAGTGTGTTTTCACTTTATTACCTCTTCCATTTAGATGGAAAATGAAAATTGGGAAGAAAAAAAAAGAAAGAAAGAAAGAAGAAGAAGCAAGAATGTTTTATTTTTTGGTACTTGACAAGGATGAAAATAAAATGAACTAAATCAAACAACTGACTGGGGGATGGAGGGAGAGAATGTGTATGGATACTTGATGGATATATTATATTTGGTAGAAACAGGAAGAAGAAAACGAAACTTTAGAAAGAATTGCATGACGATGTAGGAACAAAATCCAGTAGCCCTAGATGTTGCAGAAGTGAAACAGAGTTTTCTTTTTTTTTTTTGTTACTTATAATTTCTATCATCTTTATTAATATATGTTGGGGGTGCTTCTTATAATCACCCATTTTGTGGCATTGGAGATGTCCTTTTAGCCTGCTCCTTGTAACGTTGGATTCTAACTAGTTATTTTGTTTCTTACATGTGTCTGTTCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGTGTGTGCATCTGTATATTTATAACTGTCTTCTGTTTAGTGATAGAACAGCGATAAATTTAGACCTTAGAGAGCGTTGGTTTCTTGCAGGTCCCACCTAGCTTAATTTTTATGCCTCAAGCCTTTTATTGGGCTTCAAGAGTGAGCAAACGCTTCTGTCGTACTAGCAAGATCTTTGGTTTATCATCTGTTGTAAGTGGTAGCGGACATCATTGTAGACTTTTCAGTGCTATGAAAGAGATTAAAGTGAAAGGTGGTAGTAATGCAGATCCCCATCTGTTGAACGATGAAGCACATGCACCGCAATTGTCTTGTGCAAACCAGGGATGTTCAGCATCAAATGTGCAGTGCACCCCTGAGATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCAATCGCTCCCCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTGTCCTCATTACCTCGAGGGTCTCTCATACTGGATGCTGGTTGTGGTAATGGAAAGTACTTGGGTTTTAATCTCAACTGCTTTTTTATTGGATGTGATATTAGTCCCCAACTTATCAAAATTTGTAACGAAAGAGGACATGAAGTTTTGGTAGCTGACGCTGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTGAGTACCGAGAACAGGAGGAAGAAAGCAATTGAAGAATTATTACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGACAAATCTTTATTGACGAAATGGATGCCACTTTCAGAGAAGTATGTTGAAGAGTGGGTTGGACCTGGCAGCCCCCGTGTTCGTAGTCCATCGTCTTTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATAATTCAGGTACTTATCTAAAGGATTCCAAGGTAAACTTTACTGGAAGCATGCCGGGAAATATACCTCTACCCTCTCAAAGTGGAAATGATCTTGCCAATTGCAATGATGAAAATTTATGGAAGACCCAACAAGAGTATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCGGTGCCCTTGCTAGTGGTTTGGCAAAGAAAGATGACAAGAAAGCTGCTGTTGTCTATAACAGATACTATCATGTTTTCAGCGAAGGCGAGCTTGAAAGGTATTCCTCTCCTTCATCTCTCTTGTATAATATACTTTTTCAGTATGCGTGAGTCTTTTGAATACTTCTCATTTCGGACGGGACTGTGCTGTTCTAGATCTGTCTTTAATTCGCTCCCACTGATACTTGATGCATTGCATAAACCATAAACTTTTAGTCAGCTGGATGAAAGTCGGAACAAAGGGGGATAACTGTTAACCGGGATGTGTGACTAAAGTTGAAAGAAAGAAGCACCAGTAGTGTGTGACTAAGTAATAACTGTTAACCGGGATCATTCCCCATTTTGGCATCATTCCCATCTTTCGACTGAAGATTCCATGACTCTGTAGAAATACGGAACTCAGTATATCGTGCTTCAAGAAGAATTCTTATCTGACTTCCTTGTTTACCTTGGGGTGTATGTGCAGGTTGGTTTCTGGTATGGACAATGCAGTAGTGGTGGATCGATTTTATGACAAATCAAACTGGTGTATAGTTCTTGAGAAGACG

mRNA sequence

TTAACTAAAAATTTTGACAACTCTGCAAATGAATGTACAGTGAAGCTAGGGCACTCTTCCCCCTTCACTTCGCGACGAAGACGACCAGCCATTTGAAGCTGCCATTGCGAGATTAGTGCCTTGATTTTCTCTTATGATTCTTCTTCTTCTTTCTTTTTCCAATCATGAACTAGATTCCACAATCTCGACCTTCCATTTGATTCTTTCTAATCTTTCAGCACAATCAGGCAGCCGCGCCGTACCTGAGTTTCGTCCCACCTAGCTTAATTTTTATGCCTCAAGCCTTTTATTGGGCTTCAAGAGTGAGCAAACGCTTCTGTCGTACTAGCAAGATCTTTGGTTTATCATCTGTTGTAAGTGGTAGCGGACATCATTGTAGACTTTTCAGTGCTATGAAAGAGATTAAAGTGAAAGGTGGTAGTAATGCAGATCCCCATCTGTTGAACGATGAAGCACATGCACCGCAATTGTCTTGTGCAAACCAGGGATGTTCAGCATCAAATGTGCAGTGCACCCCTGAGATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCAATCGCTCCCCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTGTCCTCATTACCTCGAGGGTCTCTCATACTGGATGCTGGTTGTGGTAATGGAAAGTACTTGGGTTTTAATCTCAACTGCTTTTTTATTGGATGTGATATTAGTCCCCAACTTATCAAAATTTGTAACGAAAGAGGACATGAAGTTTTGGTAGCTGACGCTGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTGAGTACCGAGAACAGGAGGAAGAAAGCAATTGAAGAATTATTACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGACAAATCTTTATTGACGAAATGGATGCCACTTTCAGAGAAGTATGTTGAAGAGTGGGTTGGACCTGGCAGCCCCCGTGTTCGTAGTCCATCGTCTTTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATAATTCAGGTACTTATCTAAAGGATTCCAAGGTAAACTTTACTGGAAGCATGCCGGGAAATATACCTCTACCCTCTCAAAGTGGAAATGATCTTGCCAATTGCAATGATGAAAATTTATGGAAGACCCAACAAGAGTATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCGGTGCCCTTGCTAGTGGTTTGGCAAAGAAAGATGACAAGAAAGCTGCTGTTGTCTATAACAGATACTATCATGTTTTCAGCGAAGGCGAGCTTGAAAGGTTGGTTTCTGGTATGGACAATGCAGTAGTGGTGGATCGATTTTATGACAAATCAAACTGGTGTATAGTTCTTGAGAAGACG

Coding sequence (CDS)

ATGCCTCAAGCCTTTTATTGGGCTTCAAGAGTGAGCAAACGCTTCTGTCGTACTAGCAAGATCTTTGGTTTATCATCTGTTGTAAGTGGTAGCGGACATCATTGTAGACTTTTCAGTGCTATGAAAGAGATTAAAGTGAAAGGTGGTAGTAATGCAGATCCCCATCTGTTGAACGATGAAGCACATGCACCGCAATTGTCTTGTGCAAACCAGGGATGTTCAGCATCAAATGTGCAGTGCACCCCTGAGATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCAATCGCTCCCCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTGTCCTCATTACCTCGAGGGTCTCTCATACTGGATGCTGGTTGTGGTAATGGAAAGTACTTGGGTTTTAATCTCAACTGCTTTTTTATTGGATGTGATATTAGTCCCCAACTTATCAAAATTTGTAACGAAAGAGGACATGAAGTTTTGGTAGCTGACGCTGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTGAGTACCGAGAACAGGAGGAAGAAAGCAATTGAAGAATTATTACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGACAAATCTTTATTGACGAAATGGATGCCACTTTCAGAGAAGTATGTTGAAGAGTGGGTTGGACCTGGCAGCCCCCGTGTTCGTAGTCCATCGTCTTTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATAATTCAGGTACTTATCTAAAGGATTCCAAGGTAAACTTTACTGGAAGCATGCCGGGAAATATACCTCTACCCTCTCAAAGTGGAAATGATCTTGCCAATTGCAATGATGAAAATTTATGGAAGACCCAACAAGAGTATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCGGTGCCCTTGCTAGTGGTTTGGCAAAGAAAGATGACAAGAAAGCTGCTGTTGTCTATAACAGATACTATCATGTTTTCAGCGAAGGCGAGCTTGAAAGGTTGGTTTCTGGTATGGACAATGCAGTAGTGGTGGATCGATTTTATGACAAATCAAACTGGTGTATAGTTCTTGAGAAGACG

Protein sequence

MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDEAHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLANCNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Homology
BLAST of Carg22851 vs. NCBI nr
Match: XP_022939521.1 (alkylated DNA repair protein alkB homolog 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 808.1 bits (2086), Expect = 3.2e-230
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 54  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 353

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 354 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 413

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 414 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 444

BLAST of Carg22851 vs. NCBI nr
Match: XP_022939523.1 (alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 808.1 bits (2086), Expect = 3.2e-230
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 391

BLAST of Carg22851 vs. NCBI nr
Match: KAG6578341.1 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808.1 bits (2086), Expect = 3.2e-230
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 22  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 81

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 82  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 141

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 142 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 201

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 202 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 261

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN
Sbjct: 262 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 321

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 322 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 381

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 382 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 412

BLAST of Carg22851 vs. NCBI nr
Match: KAG7015918.1 (Alkylated DNA repair protein alkB-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 808.1 bits (2086), Expect = 3.2e-230
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 391

BLAST of Carg22851 vs. NCBI nr
Match: XP_022993245.1 (alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 796.6 bits (2056), Expect = 9.8e-227
Identity = 386/391 (98.72%), Postives = 386/391 (98.72%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFC TSKIFGLSSVVSGS HHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCHTSKIFGLSSVVSGSVHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMP NIPLPSQ GND AN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPENIPLPSQGGNDFAN 300

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 391

BLAST of Carg22851 vs. ExPASy Swiss-Prot
Match: Q94A09 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=TRM9 PE=1 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 8.0e-131
Identity = 246/381 (64.57%), Postives = 284/381 (74.54%), Query Frame = 0

Query: 31  SGHHCRLFSAMKEIKVKGGSNADPHLLNDEAHAPQLSCANQGCSASNVQCTPEIEKKYVH 90
           SG +    S M++IKVK  S       ++E  +  +  ++   S S+V+ TPEIEKKYVH
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSS-SLSSVKSTPEIEKKYVH 78

Query: 91  RVYDAIAPHFSSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFFIGCDISP 150
           RVYDAIAPHFSSTRFAKWPKVA+FL SLP GS+ILDAGCGNGKYLG N +CFFIGCDIS 
Sbjct: 79  RVYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISH 138

Query: 151 QLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELLRVVKKG 210
            LIKIC+++G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK G
Sbjct: 139 PLIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPG 198

Query: 211 GLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPG----SPRVRSPSSLALESIPEMNE 270
           G VLITVWA EQED SLLTKW PLS KYVEEWVGPG    SPRVR+    +LESIPE   
Sbjct: 199 GFVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEV 258

Query: 271 NNSGTYLKDSKVNFTGSMP----------GNIPLPSQSGNDLANCND-------ENLWKT 330
           +     +++S+     S+P          G   +P    + +   ++       E L K+
Sbjct: 259 STKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKS 318

Query: 331 QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGM 390
           QQEYFVPWHLPYHRAEVSG SA ALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+
Sbjct: 319 QQEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGV 378

BLAST of Carg22851 vs. ExPASy Swiss-Prot
Match: Q07G10 (Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alkbh8 PE=2 SV=2)

HSP 1 Score: 225.7 bits (574), Expect = 9.0e-58
Identity = 128/311 (41.16%), Postives = 178/311 (57.23%), Query Frame = 0

Query: 84  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFF 143
           +E++YVH+VYD IA HFSSTR   WPK+  FL+SLP+GSL+ D GCGNGKYLG N +   
Sbjct: 369 LEREYVHKVYDDIAGHFSSTRHTPWPKIGDFLASLPKGSLVADVGCGNGKYLGVNKDLCM 428

Query: 144 IGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 203
           IGCD S  L+ IC ER  E  V DA+++P+R G  DA ISIAV+HH +TE RR  A++EL
Sbjct: 429 IGCDRSKNLVDICRERTFEAFVCDALSVPFRAGAFDACISIAVIHHFATEERRIAALQEL 488

Query: 204 LRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPE 263
           +R+++KGG  LI VWA+EQE K           KY++E     S   + PSS AL     
Sbjct: 489 IRLLRKGGKALIYVWALEQEYKK-------NKSKYLKE-----SKTSQGPSSDAL----- 548

Query: 264 MNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLANCNDENLWKTQQEYFVPWHL-PYH 323
                 GT           ++    PLP  +     +          Q+  VPWHL P +
Sbjct: 549 ------GT-----------NLAVKAPLPIHTNRTSFH---------SQDLLVPWHLKPTN 608

Query: 324 RAEVSGTSAGALASGLAKKDDKK---AAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFY 383
           +++V+  +         K+ ++K      VY+R+YHVF EGELE + + + N  V   ++
Sbjct: 609 KSKVTPEN---------KEQNEKEHGPDSVYHRFYHVFCEGELEAMCNRLSNVAVQHSYH 627

Query: 384 DKSNWCIVLEK 391
           D+ NWC++LEK
Sbjct: 669 DQGNWCVILEK 627

BLAST of Carg22851 vs. ExPASy Swiss-Prot
Match: Q80Y20 (Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.1e-55
Identity = 122/314 (38.85%), Postives = 177/314 (56.37%), Query Frame = 0

Query: 83  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCF 142
           E+E+K+VH+VY+ IA HFSSTR + WP++  FL +LP GS++ D GCGNGKYLG N + +
Sbjct: 369 ELEQKHVHQVYNEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKDLY 428

Query: 143 FIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEE 202
            IGCD S  L+ IC ER  + LV DA+ +P R+G  DA ISIAV+HH +T  RR +A++E
Sbjct: 429 MIGCDRSQNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQE 488

Query: 203 LLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIP 262
           L R+++ GG  LI VWA+EQE K+  +K+  L  K + +          S     +   P
Sbjct: 489 LARLLRPGGQALIYVWAMEQEYKNQKSKY--LRGKRISQGDKDELNSATSTEEFLVNQTP 548

Query: 263 E-MNEN-----NSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLANCNDENLWKTQQEYFVP 322
           E +NE+     NS +  K+ +  +      N  LP        +          Q+  VP
Sbjct: 549 EGVNEDPALSVNSSSITKEEE--YKSRKVPNSELPIHINRTCFH---------SQDVLVP 608

Query: 323 WHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVD 382
           WHL  +     G       SG+A   D   + V++RYYHVF +GELE     + +  ++ 
Sbjct: 609 WHLKRN----PGKDKAIEPSGVAGCPD--PSPVFHRYYHVFCDGELEASCQAVGDVSILQ 663

Query: 383 RFYDKSNWCIVLEK 391
            +YD+ NWC+VL+K
Sbjct: 669 SYYDQGNWCVVLQK 663

BLAST of Carg22851 vs. ExPASy Swiss-Prot
Match: A1A4L5 (Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.7e-54
Identity = 119/321 (37.07%), Postives = 172/321 (53.58%), Query Frame = 0

Query: 83  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCF 142
           ++E+ YVHRVY+ IA HFSSTR   WP +  FL +LP GSL+ D GCGNGKYLG N   +
Sbjct: 369 QLEQDYVHRVYEEIARHFSSTRHTPWPHIVEFLKALPSGSLVADIGCGNGKYLGINNELY 428

Query: 143 FIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEE 202
            IGCD S  L+ IC ER ++  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E
Sbjct: 429 MIGCDHSQNLVDICRERQYQAFVCDALAVPIRSGSCDACISIAVIHHFATAERRVAALQE 488

Query: 203 LLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIP 262
           L+R+++ GG  LI VWA+EQE     +K+  L E    + +        S   L ++ +P
Sbjct: 489 LVRLLRPGGKALIYVWAMEQEYNKKKSKY--LRENRTSQGMKEEISNDASVQELLVKQLP 548

Query: 263 EMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLANCNDENLWKTQ------------ 322
            +   +S   +  S +N             Q G     CN +N+  ++            
Sbjct: 549 NVGNQDSAHSV--SSIN-----------DFQDG----GCNSKNVANSKLPIHTNRTSFHS 608

Query: 323 QEYFVPWHLPYHRAEVSGTSA-GALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGM 382
           Q+  VPWH   +  +       G L S       +    V++RYYHVF EGELE     +
Sbjct: 609 QDLLVPWHFKGNPGKDKRVEPFGPLGS-------RDPGPVFHRYYHVFCEGELEAACRTL 663

Query: 383 DNAVVVDRFYDKSNWCIVLEK 391
           +N  ++  +YD+ NWC++L+K
Sbjct: 669 NNISILQSYYDQGNWCVILQK 663

BLAST of Carg22851 vs. ExPASy Swiss-Prot
Match: Q95K79 (Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.0e-53
Identity = 114/310 (36.77%), Postives = 171/310 (55.16%), Query Frame = 0

Query: 84  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFF 143
           +E++YVH+VY+ IA HFSSTR   WP +  FL +LP GS++ D GCGNGKYLG N   + 
Sbjct: 370 LEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKYLGVNKELYM 429

Query: 144 IGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 203
           +GCD S  L+ IC ER  +  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E+
Sbjct: 430 VGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEI 489

Query: 204 LRVVKKGGLVLITVWAVEQEDKSLLTKWMP--LSEKYVEEWVGPGSPRVRSPSSLALESI 263
           +R+++ GG  LI VWA+EQE     +K++    + +  +E +   +   RS     +E +
Sbjct: 490 VRLLRPGGKALIYVWAMEQEYNKQKSKYLKGNRNSQGKKEEMNSDTSVQRS----LVEQM 549

Query: 264 PEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDL-ANCNDENLWKTQQEYFVPWHLP 323
           P+M   +S +    S      S  G       S + L  + N  + +   Q+  VPWHL 
Sbjct: 550 PDMGSRDSAS----SVPRINDSQEGGCNSRQVSNSKLPIHVNRTSFY--SQDMLVPWHLK 609

Query: 324 YHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFYD 383
                      G           +  + V++RYYHVF EGELE L   + +  ++  +YD
Sbjct: 610 ------GNPDKGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEALCRTVSDVRILQSYYD 663

Query: 384 KSNWCIVLEK 391
           + NWC++L+K
Sbjct: 670 QGNWCVILQK 663

BLAST of Carg22851 vs. ExPASy TrEMBL
Match: A0A6J1FLV5 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.6e-230
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 391

BLAST of Carg22851 vs. ExPASy TrEMBL
Match: A0A6J1FG55 (alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.6e-230
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 54  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 353

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 354 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 413

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 414 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 444

BLAST of Carg22851 vs. ExPASy TrEMBL
Match: A0A6J1JY21 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489322 PE=4 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 4.7e-227
Identity = 386/391 (98.72%), Postives = 386/391 (98.72%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFC TSKIFGLSSVVSGS HHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCHTSKIFGLSSVVSGSVHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMP NIPLPSQ GND AN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPENIPLPSQGGNDFAN 300

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 391

BLAST of Carg22851 vs. ExPASy TrEMBL
Match: A0A6J1K1M9 (alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489322 PE=4 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 4.7e-227
Identity = 386/391 (98.72%), Postives = 386/391 (98.72%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           MPQAFYWASRVSKRFC TSKIFGLSSVVSGS HHCRLFSAMKEIKVKGGSNADPHLLNDE
Sbjct: 54  MPQAFYWASRVSKRFCHTSKIFGLSSVVSGSVHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
           AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMP NIPLPSQ GND AN
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPENIPLPSQGGNDFAN 353

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 354 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 413

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 414 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 444

BLAST of Carg22851 vs. ExPASy TrEMBL
Match: A0A0A0LMT8 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G403730 PE=4 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.7e-200
Identity = 351/390 (90.00%), Postives = 362/390 (92.82%), Query Frame = 0

Query: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60
           M Q+F  +SRVSK FC + KIFGLSSVV GSGHH RL SAMKEIK+KGGSNADPH LNDE
Sbjct: 1   MLQSFDRSSRVSKYFCHSCKIFGLSSVVIGSGHHFRLNSAMKEIKIKGGSNADPHPLNDE 60

Query: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120
            HA QLSCA QGC+ASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLP 
Sbjct: 61  THA-QLSCAIQGCTASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPL 120

Query: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSL+LDAGCGNGKYLG+N NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLVLDAGCGNGKYLGYNSNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300
           EWVGPGSPRVRSPSS+ALESIPEMNENNSG  LKDSK N TGS+P N P PSQS NDLAN
Sbjct: 241 EWVGPGSPRVRSPSSMALESIPEMNENNSGICLKDSKENLTGSIPENKPPPSQSENDLAN 300

Query: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CN ENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNHENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEK 391
           ELE L+SGMDNAVVVDRFYDKSNWCIVLEK
Sbjct: 361 ELEGLISGMDNAVVVDRFYDKSNWCIVLEK 389

BLAST of Carg22851 vs. TAIR 10
Match: AT1G36310.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 468.4 bits (1204), Expect = 5.7e-132
Identity = 246/381 (64.57%), Postives = 284/381 (74.54%), Query Frame = 0

Query: 31  SGHHCRLFSAMKEIKVKGGSNADPHLLNDEAHAPQLSCANQGCSASNVQCTPEIEKKYVH 90
           SG +    S M++IKVK  S       ++E  +  +  ++   S S+V+ TPEIEKKYVH
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSS-SLSSVKSTPEIEKKYVH 78

Query: 91  RVYDAIAPHFSSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFFIGCDISP 150
           RVYDAIAPHFSSTRFAKWPKVA+FL SLP GS+ILDAGCGNGKYLG N +CFFIGCDIS 
Sbjct: 79  RVYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISH 138

Query: 151 QLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELLRVVKKG 210
            LIKIC+++G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK G
Sbjct: 139 PLIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPG 198

Query: 211 GLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPG----SPRVRSPSSLALESIPEMNE 270
           G VLITVWA EQED SLLTKW PLS KYVEEWVGPG    SPRVR+    +LESIPE   
Sbjct: 199 GFVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEV 258

Query: 271 NNSGTYLKDSKVNFTGSMP----------GNIPLPSQSGNDLANCND-------ENLWKT 330
           +     +++S+     S+P          G   +P    + +   ++       E L K+
Sbjct: 259 STKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKS 318

Query: 331 QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGM 390
           QQEYFVPWHLPYHRAEVSG SA ALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+
Sbjct: 319 QQEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGV 378

BLAST of Carg22851 vs. TAIR 10
Match: AT1G36310.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 468.4 bits (1204), Expect = 5.7e-132
Identity = 246/381 (64.57%), Postives = 284/381 (74.54%), Query Frame = 0

Query: 31  SGHHCRLFSAMKEIKVKGGSNADPHLLNDEAHAPQLSCANQGCSASNVQCTPEIEKKYVH 90
           SG +    S M++IKVK  S       ++E  +  +  ++   S S+V+ TPEIEKKYVH
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSS-SLSSVKSTPEIEKKYVH 78

Query: 91  RVYDAIAPHFSSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFFIGCDISP 150
           RVYDAIAPHFSSTRFAKWPKVA+FL SLP GS+ILDAGCGNGKYLG N +CFFIGCDIS 
Sbjct: 79  RVYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISH 138

Query: 151 QLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELLRVVKKG 210
            LIKIC+++G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK G
Sbjct: 139 PLIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPG 198

Query: 211 GLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPG----SPRVRSPSSLALESIPEMNE 270
           G VLITVWA EQED SLLTKW PLS KYVEEWVGPG    SPRVR+    +LESIPE   
Sbjct: 199 GFVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEV 258

Query: 271 NNSGTYLKDSKVNFTGSMP----------GNIPLPSQSGNDLANCND-------ENLWKT 330
           +     +++S+     S+P          G   +P    + +   ++       E L K+
Sbjct: 259 STKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKS 318

Query: 331 QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGM 390
           QQEYFVPWHLPYHRAEVSG SA ALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+
Sbjct: 319 QQEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGV 378

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022939521.13.2e-230100.00alkylated DNA repair protein alkB homolog 8-like isoform X1 [Cucurbita moschata][more]
XP_022939523.13.2e-230100.00alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita moschata][more]
KAG6578341.13.2e-230100.00tRNA (carboxymethyluridine(34)-5-O)-methyltransferase, partial [Cucurbita argyro... [more]
KAG7015918.13.2e-230100.00Alkylated DNA repair protein alkB-like 8, partial [Cucurbita argyrosperma subsp.... [more]
XP_022993245.19.8e-22798.72alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94A098.0e-13164.57tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX... [more]
Q07G109.0e-5841.16Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alk... [more]
Q80Y201.1e-5538.85Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 P... [more]
A1A4L52.7e-5437.07Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2... [more]
Q95K793.0e-5336.77Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=AL... [more]
Match NameE-valueIdentityDescription
A0A6J1FLV51.6e-230100.00alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschat... [more]
A0A6J1FG551.6e-230100.00alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita moschat... [more]
A0A6J1JY214.7e-22798.72alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima ... [more]
A0A6J1K1M94.7e-22798.72alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita maxima ... [more]
A0A0A0LMT81.7e-20090.00Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40... [more]
Match NameE-valueIdentityDescription
AT1G36310.15.7e-13264.57S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G36310.25.7e-13264.57S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 100..391
e-value: 4.6E-59
score: 201.3
NoneNo IPR availablePANTHERPTHR13069:SF33BNAA05G09210D PROTEINcoord: 75..267
coord: 308..385
NoneNo IPR availablePANTHERPTHR13069UNCHARACTERIZEDcoord: 75..267
NoneNo IPR availablePANTHERPTHR13069UNCHARACTERIZEDcoord: 308..385
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 124..220
e-value: 4.71605E-10
score: 54.3583
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 125..215
e-value: 6.5E-15
score: 55.6
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 80..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22851-RACarg22851-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002098 tRNA wobble uridine modification
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000049 tRNA binding