Carg22694 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg22694 vs. NCBI nr
Match: KAG7022165.1 (DnaJ-like subfamily C member 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 437.2 bits (1123), Expect = 8.9e-119
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK
Sbjct: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW
Sbjct: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
           LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT
Sbjct: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVSI 232
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVSI
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVSI 231

BLAST of Carg22694 vs. NCBI nr
Match: XP_022933700.1 (dnaJ homolog subfamily C member 8-like [Cucurbita moschata] >XP_023520681.1 dnaJ homolog subfamily C member 8-like [Cucurbita pepo subsp. pepo] >KAG6588248.1 DnaJ-like subfamily C member 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 380.6 bits (976), Expect = 9.9e-102
Identity = 210/230 (91.30%), Postives = 212/230 (92.17%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK
Sbjct: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                    +ELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT
Sbjct: 121 ---------EELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. NCBI nr
Match: XP_022965396.1 (dnaJ homolog subfamily C member 8-like [Cucurbita maxima])

HSP 1 Score: 379.0 bits (972), Expect = 2.9e-101
Identity = 209/230 (90.87%), Postives = 211/230 (91.74%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFD SPEEVK
Sbjct: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDVSPEEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                    +ELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT
Sbjct: 121 ---------EELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. NCBI nr
Match: XP_022141953.1 (dnaJ homolog subfamily C member 8 [Momordica charantia])

HSP 1 Score: 370.5 bits (950), Expect = 1.0e-98
Identity = 204/230 (88.70%), Postives = 210/230 (91.30%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANK+TST EEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASP+EVK
Sbjct: 1   MGEANKTTSTPEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPDEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQAKEAFGALAKAQ+LLLDEQERDYILSQV+AAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQAKEAFGALAKAQQLLLDEQERDYILSQVSAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                     ELLAKRKKQLKKDTASKIKSLVEEGKY+QQYESTEEFKQELKLKVREILT
Sbjct: 121 ---------DELLAKRKKQLKKDTASKIKSLVEEGKYEQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. NCBI nr
Match: XP_004139080.1 (dnaJ homolog subfamily C member 8 [Cucumis sativus] >KGN66598.1 hypothetical protein Csa_007641 [Cucumis sativus])

HSP 1 Score: 368.6 bits (945), Expect = 3.9e-98
Identity = 202/230 (87.83%), Postives = 210/230 (91.30%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANKSTSTAEEDLLLK+FFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDA+PEEVK
Sbjct: 1   MGEANKSTSTAEEDLLLKTFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDATPEEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQ+KEAF ALAKAQ+LLLDEQERDYILSQVNAAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQSKEAFAALAKAQQLLLDEQERDYILSQVNAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                    +ELLAKRKK LK+DTASKIKSLVEEGKY+QQYESTEEFKQELKLKVREILT
Sbjct: 121 ---------EELLAKRKKHLKRDTASKIKSLVEEGKYEQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. ExPASy Swiss-Prot
Match: O75937 (DnaJ homolog subfamily C member 8 OS=Homo sapiens OX=9606 GN=DNAJC8 PE=1 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 3.3e-15
Identity = 74/241 (30.71%), Postives = 131/241 (54.36%), Query Frame = 0

Query: 2   GEANKSTSTAEEDLLLKSFFAEVSEVER-------DNEVIRIL----SCFKLNPFEYLKL 61
           GE+  S      +    +F++EV ++E+        N++ R+     S F LNPFE L++
Sbjct: 5   GESGTSGGGGSTEEAFMTFYSEVKQIEKRDSVLTSKNQIERLTRPGSSYFNLNPFEVLQI 64

Query: 62  PFDASPEEVKKQYRKLSLLVHPDKCKH--PQAKEAFGALAKAQKLLLDEQERDYILSQVN 121
             + + EE+KK++R+LS+LVHPDK +    +A++AF A+ KA KLLLD++++   L  + 
Sbjct: 65  DPEVTDEEIKKRFRQLSILVHPDKNQDDADRAQKAFEAVDKAYKLLLDQEQKKRALDVIQ 124

Query: 122 AAKGNVLLPSVWLSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFK 181
           A K  V               +  + +RKKQLKK+      ++VEE       +  E FK
Sbjct: 125 AGKEYV---------------EHTVKERKKQLKKEGK---PTIVEE-------DDPELFK 184

Query: 182 QELKLKVREILTEQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQR 230
           Q +  +  ++  E E +R++ + +   E  R +++E E +E  K++RE ++ +E +R+ R
Sbjct: 185 QAVYKQTMKLFAELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDGR 220

BLAST of Carg22694 vs. ExPASy Swiss-Prot
Match: Q6NZB0 (DnaJ homolog subfamily C member 8 OS=Mus musculus OX=10090 GN=Dnajc8 PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 1.2e-14
Identity = 74/242 (30.58%), Postives = 130/242 (53.72%), Query Frame = 0

Query: 2   GEANKSTSTAEEDLLLKSFFAEVSEVER-------DNEVIRIL----SCFKLNPFEYLKL 61
           GE+  S      +    +F++EV ++E+        N++ R+     S F LNPFE L++
Sbjct: 5   GESGASGGGGSTEEAFMTFYSEVKQIEKRDSVLTSKNQIERLTRPGSSYFNLNPFEVLQI 64

Query: 62  PFDASPEEVKKQYRKLSLLVHPDKCKH--PQAKEAFGALAKAQKLLLDEQERDYILSQVN 121
             + + EE+KK++R+LS+LVHPDK +    +A++AF A+ KA KLLLD++++   L  + 
Sbjct: 65  DPEVTDEEIKKRFRQLSILVHPDKNQDDADRAQKAFEAVDKAYKLLLDQEQKKRALDVIQ 124

Query: 122 AAKGNVLLPSVWLSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGK-YDQQYESTEEF 181
           A K  V               +  + +RKKQLKK           EGK  + + +  E F
Sbjct: 125 AGKEYV---------------EHTVKERKKQLKK-----------EGKPTNVEEDDPELF 184

Query: 182 KQELKLKVREILTEQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQ 230
           KQ +  +  ++  E E +R++ + +   E  R +++E E +E  K++RE ++ +E +R+ 
Sbjct: 185 KQAVYKQTMKLFAELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDG 220

BLAST of Carg22694 vs. ExPASy Swiss-Prot
Match: Q642C0 (DnaJ homolog subfamily C member 8 OS=Rattus norvegicus OX=10116 GN=Dnajc8 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.2e-14
Identity = 74/242 (30.58%), Postives = 130/242 (53.72%), Query Frame = 0

Query: 2   GEANKSTSTAEEDLLLKSFFAEVSEVER-------DNEVIRIL----SCFKLNPFEYLKL 61
           GE+  S      +    +F++EV ++E+        N++ R+     S F LNPFE L++
Sbjct: 5   GESGASGGGGSTEEAFMTFYSEVKQIEKRDSVLTSKNQIERLTRPGSSYFNLNPFEVLQI 64

Query: 62  PFDASPEEVKKQYRKLSLLVHPDKCKH--PQAKEAFGALAKAQKLLLDEQERDYILSQVN 121
             + + EE+KK++R+LS+LVHPDK +    +A++AF A+ KA KLLLD++++   L  + 
Sbjct: 65  DPEVTDEEIKKRFRQLSILVHPDKNQDDADRAQKAFEAVDKAYKLLLDQEQKKRALDVIQ 124

Query: 122 AAKGNVLLPSVWLSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGK-YDQQYESTEEF 181
           A K  V               +  + +RKKQLKK           EGK  + + +  E F
Sbjct: 125 AGKEYV---------------EHTVKERKKQLKK-----------EGKPTNVEEDDPELF 184

Query: 182 KQELKLKVREILTEQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQ 230
           KQ +  +  ++  E E +R++ + +   E  R +++E E +E  K++RE ++ +E +R+ 
Sbjct: 185 KQAVYKQTMKLFAELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDG 220

BLAST of Carg22694 vs. ExPASy Swiss-Prot
Match: O74746 (J domain-containing protein spf31 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spf31 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-08
Identity = 59/205 (28.78%), Postives = 107/205 (52.20%), Query Frame = 0

Query: 27  VERDNEVIRILSCFKLNPFEYLKLPFDASPEEVKKQYRKLSLLVHPDKCK-HPQAKEAFG 86
           + R  E+ RILS FKLN ++ L +    S ++++  YRK SL++HPDK + +P+A +AF 
Sbjct: 15  LNRGREIDRILSSFKLNAYDVLDILPGMSVDDIRNLYRKKSLMIHPDKNRDNPKAADAFD 74

Query: 87  ALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVWLSNLVHIWCKELLAKRKKQLKKDTA 146
            L KA+  L++++ R+ + S   AA+  +L                    ++K+L  ++ 
Sbjct: 75  ILKKAESDLVNDKIRESLDSAYTAARNQLL--------------------KEKKLSPNS- 134

Query: 147 SKIKSLVEEGKYDQQYESTEEFKQELKLKVREILTEQE-WRRRKMQMRISEEEGRLKKDE 206
                         +   +++F  +LK++ REIL   E  RRR  Q+ ++ ++    + +
Sbjct: 135 --------------EDVHSDQFLFDLKVRWREILIADEVARRRARQLDLANQQREQARQD 183

Query: 207 EETKEMWKKKREHEEQWEGTREQRV 230
           E  +E  K++ E E+ WE TR+ RV
Sbjct: 195 EIARER-KRRVESEKVWEETRDNRV 183

BLAST of Carg22694 vs. ExPASy Swiss-Prot
Match: Q17438 (DnaJ homolog subfamily B member 1 OS=Caenorhabditis elegans OX=6239 GN=dnj-1 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.1e-08
Identity = 32/73 (43.84%), Postives = 46/73 (63.01%), Query Frame = 0

Query: 28  ERDNEVIRILSCFKLNPFEYLKLPFDASPEEVKKQYRKLSLLVHPDKCKHPQAKEAFGAL 87
           E+   V RI  C   + +E LK+   AS ++++K+YRKL+L +HPDKC+ P A EAF AL
Sbjct: 124 EQKELVERIRHC--KDYYEILKIDKKASDDDIRKEYRKLALKLHPDKCRAPHATEAFKAL 183

Query: 88  AKAQKLLLDEQER 101
             A  +L D  +R
Sbjct: 184 GNAYAVLSDTDKR 194

BLAST of Carg22694 vs. ExPASy TrEMBL
Match: A0A6J1F5K2 (dnaJ homolog subfamily C member 8-like OS=Cucurbita moschata OX=3662 GN=LOC111441036 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.8e-102
Identity = 210/230 (91.30%), Postives = 212/230 (92.17%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK
Sbjct: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                    +ELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT
Sbjct: 121 ---------EELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. ExPASy TrEMBL
Match: A0A6J1HLK2 (dnaJ homolog subfamily C member 8-like OS=Cucurbita maxima OX=3661 GN=LOC111465312 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.4e-101
Identity = 209/230 (90.87%), Postives = 211/230 (91.74%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFD SPEEVK
Sbjct: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDVSPEEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                    +ELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT
Sbjct: 121 ---------EELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. ExPASy TrEMBL
Match: A0A6J1CM19 (dnaJ homolog subfamily C member 8 OS=Momordica charantia OX=3673 GN=LOC111012202 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.9e-99
Identity = 204/230 (88.70%), Postives = 210/230 (91.30%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANK+TST EEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASP+EVK
Sbjct: 1   MGEANKTTSTPEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPDEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQAKEAFGALAKAQ+LLLDEQERDYILSQV+AAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQAKEAFGALAKAQQLLLDEQERDYILSQVSAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                     ELLAKRKKQLKKDTASKIKSLVEEGKY+QQYESTEEFKQELKLKVREILT
Sbjct: 121 ---------DELLAKRKKQLKKDTASKIKSLVEEGKYEQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. ExPASy TrEMBL
Match: A0A0A0LXW6 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G637960 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.9e-98
Identity = 202/230 (87.83%), Postives = 210/230 (91.30%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MGEANKSTSTAEEDLLLK+FFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDA+PEEVK
Sbjct: 1   MGEANKSTSTAEEDLLLKTFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDATPEEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQ+KEAF ALAKAQ+LLLDEQERDYILSQVNAAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQSKEAFAALAKAQQLLLDEQERDYILSQVNAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                    +ELLAKRKK LK+DTASKIKSLVEEGKY+QQYESTEEFKQELKLKVREILT
Sbjct: 121 ---------EELLAKRKKHLKRDTASKIKSLVEEGKYEQQYESTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. ExPASy TrEMBL
Match: A0A6J1H9X0 (dnaJ homolog subfamily C member 8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461822 PE=4 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 3.9e-96
Identity = 198/230 (86.09%), Postives = 207/230 (90.00%), Query Frame = 0

Query: 1   MGEANKSTSTAEEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVK 60
           MG+ NKSTSTAEEDLLLK+FFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDA+PEEVK
Sbjct: 1   MGDPNKSTSTAEEDLLLKTFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDATPEEVK 60

Query: 61  KQYRKLSLLVHPDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVW 120
           +QYRKLSLLVHPDKCKHPQ+KEAF ALAKAQ+LLLDEQER YILSQVNAAK         
Sbjct: 61  RQYRKLSLLVHPDKCKHPQSKEAFAALAKAQQLLLDEQERGYILSQVNAAK--------- 120

Query: 121 LSNLVHIWCKELLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILT 180
                    +ELLAKRKK LKKDTASKIKSLVEEGKY+QQYE TEEFKQELKLKVREILT
Sbjct: 121 ---------EELLAKRKKHLKKDTASKIKSLVEEGKYEQQYERTEEFKQELKLKVREILT 180

Query: 181 EQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           +QEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS
Sbjct: 181 DQEWRRRKMQMRISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 212

BLAST of Carg22694 vs. TAIR 10
Match: AT5G22080.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 1.9e-79
Identity = 160/219 (73.06%), Postives = 186/219 (84.93%), Query Frame = 0

Query: 12  EEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVKKQYRKLSLLVH 71
           ++D +LKSF AEV EVERDNEV RILSCFKLNPFE+L L FD+S ++VK+QYRK+SL+VH
Sbjct: 7   DDDAILKSFLAEVGEVERDNEVGRILSCFKLNPFEHLNLSFDSSTDDVKRQYRKISLMVH 66

Query: 72  PDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVWLSNLVHIWCKE 131
           PDKCKHPQA+EAFGALAKAQ+LLL++QERDYIL+QV+AAK                  +E
Sbjct: 67  PDKCKHPQAQEAFGALAKAQQLLLNDQERDYILTQVHAAK------------------EE 126

Query: 132 LLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILTEQEWRRRKMQM 191
           L  KRKKQLKKDTASKIKSLV+EGK++  YE +EEF++ELKLKVREILT+QEWRRRKM M
Sbjct: 127 LKMKRKKQLKKDTASKIKSLVDEGKHEHIYEQSEEFQKELKLKVREILTDQEWRRRKMAM 186

Query: 192 RISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
           RISEEEGRLKKDE E KE+WKKKREHEEQWEGTRE+RVS
Sbjct: 187 RISEEEGRLKKDEAEQKEIWKKKREHEEQWEGTREKRVS 207

BLAST of Carg22694 vs. TAIR 10
Match: AT5G22080.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 253.8 bits (647), Expect = 1.3e-67
Identity = 144/219 (65.75%), Postives = 169/219 (77.17%), Query Frame = 0

Query: 12  EEDLLLKSFFAEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVKKQYRKLSLLVH 71
           ++D +LKSF AEV EVERDNEV RILSCFKLNPFE+L L FD+S ++VK+QYRK+SL+VH
Sbjct: 7   DDDAILKSFLAEVGEVERDNEVGRILSCFKLNPFEHLNLSFDSSTDDVKRQYRKISLMVH 66

Query: 72  PDKCKHPQAKEAFGALAKAQKLLLDEQERDYILSQVNAAKGNVLLPSVWLSNLVHIWCKE 131
           PDKCKHPQA+EAFGALAKAQ+LLL++QERDYIL+QV+AAK                  +E
Sbjct: 67  PDKCKHPQAQEAFGALAKAQQLLLNDQERDYILTQVHAAK------------------EE 126

Query: 132 LLAKRKKQLKKDTASKIKSLVEEGKYDQQYESTEEFKQELKLKVREILTEQEWRRRKMQM 191
           L  KRKKQLKKDTASKIKSLV+EGK++  YE +EEF++ELKLK                 
Sbjct: 127 LKMKRKKQLKKDTASKIKSLVDEGKHEHIYEQSEEFQKELKLK----------------- 186

Query: 192 RISEEEGRLKKDEEETKEMWKKKREHEEQWEGTREQRVS 231
            ISEEEGRLKKDE E KE+WKKKREHEEQWEGTRE+RVS
Sbjct: 187 -ISEEEGRLKKDEAEQKEIWKKKREHEEQWEGTREKRVS 189

BLAST of Carg22694 vs. TAIR 10
Match: AT1G65280.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 72.0 bits (175), Expect = 7.0e-13
Identity = 38/79 (48.10%), Postives = 52/79 (65.82%), Query Frame = 0

Query: 22  AEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVKKQYRKLSLLVHPDKCKHPQAK 81
           A  +E ER  EV RI+     +P++ L +  + + + +KK+Y KLSLLVHPDKC HPQA+
Sbjct: 284 ASSNEAERFEEVTRIMEADANSPYDVLGVNHNMAADNMKKRYWKLSLLVHPDKCSHPQAQ 343

Query: 82  EAFGALAKAQKLLLDEQER 101
           EAF  L KA K L D ++R
Sbjct: 344 EAFVLLNKAFKELQDPEKR 362

BLAST of Carg22694 vs. TAIR 10
Match: AT5G05750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.2e-07
Identity = 31/76 (40.79%), Postives = 47/76 (61.84%), Query Frame = 0

Query: 25  SEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVKKQYRKLSLLVHPDKCKHPQAKEAF 84
           S  E    ++R +   K + +E L L  + S E+++K YRKLSL VHPDK K P ++EAF
Sbjct: 97  SSTEEQRTIVREIKS-KKDYYEILGLKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSEEAF 156

Query: 85  GALAKAQKLLLDEQER 101
            +++KA + L +E  R
Sbjct: 157 KSVSKAFQCLSNEDTR 171

BLAST of Carg22694 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 52.0 bits (123), Expect = 7.5e-07
Identity = 33/85 (38.82%), Postives = 52/85 (61.18%), Query Frame = 0

Query: 22  AEVSEVERDNEVIRILSCFKLNPFEYLKLPFDASPEEVKKQYRKLSLLVHPDKCKHPQAK 81
           + +S  E    ++R +   K + +E L L  + S ++V+K YRKLSL VHPDK + P ++
Sbjct: 93  SSMSYTEEQISIVRKIKS-KKDYYEILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSE 152

Query: 82  EAFGALAKA-QKLLLDEQERDYILS 106
           EAF +++KA Q L  DE  + Y +S
Sbjct: 153 EAFKSVSKAFQCLSNDEARKKYDVS 176