Carg22516 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg22516 vs. NCBI nr
Match: KAG7020247.1 (Beta carbonic anhydrase 5, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716.5 bits (1848), Expect = 1.2e-202
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 351

BLAST of Carg22516 vs. NCBI nr
Match: XP_023537827.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634.0 bits (1634), Expect = 7.5e-178
Identity = 315/351 (89.74%), Postives = 318/351 (90.60%), Query Frame = 0

Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEID+NH+EVPSNR
Sbjct: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             LKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NT-----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 322

BLAST of Carg22516 vs. NCBI nr
Match: XP_022951348.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 633.6 bits (1633), Expect = 9.8e-178
Identity = 316/351 (90.03%), Postives = 318/351 (90.60%), Query Frame = 0

Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NH+EV SNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             LKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NT-----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 322

BLAST of Carg22516 vs. NCBI nr
Match: XP_023001870.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 625.5 bits (1612), Expect = 2.7e-175
Identity = 312/351 (88.89%), Postives = 316/351 (90.03%), Query Frame = 0

Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NHMEVPSNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHMEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
            HELEVSRNSLGLAQERSS MGVKNVAQVKDADKVFDEMKMRFLSFKKH YL+NLEHFQA
Sbjct: 61  YHELEVSRNSLGLAQERSSWMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLENLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             LKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NT-----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWM NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMANAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAG+DDHGFQIKNQS+WC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGNDDHGFQIKNQSLWC 322

BLAST of Carg22516 vs. NCBI nr
Match: XP_023537825.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.2 bits (1611), Expect = 3.5e-175
Identity = 311/348 (89.37%), Postives = 314/348 (90.23%), Query Frame = 0

Query: 4   LIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHE 63
           LI PRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEID+NH+EVPSNRNHE
Sbjct: 46  LIGPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNRNHE 105

Query: 64  LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAE 123
           LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQALAE
Sbjct: 106 LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAE 165

Query: 124 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTL 183
           LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAVNT 
Sbjct: 166 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNT- 225

Query: 184 KVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSL 243
                                       LKVENILVIGHSSCAGIQSLMSMEDDATDSSL
Sbjct: 226 ----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATDSSL 285

Query: 244 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 303
           FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK
Sbjct: 286 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 345

Query: 304 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 346 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 364

BLAST of Carg22516 vs. ExPASy Swiss-Prot
Match: Q94CE3 (Beta carbonic anhydrase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA5 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-73
Identity = 148/290 (51.03%), Postives = 194/290 (66.90%), Query Frame = 0

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           N ++  S  + GL QE +   GV  + +  + D VFD+MK RFL+FKK  Y+ + EH++ 
Sbjct: 52  NLQVMASGKTPGLTQEAN---GVA-IDRQNNTD-VFDDMKQRFLAFKKLKYMDDFEHYKN 111

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LA+ QAPKF+VI+C DSRVCPS +LGFQPG+AFTVRN+AN+VP +E+GPTETKAALEF+V
Sbjct: 112 LADAQAPKFLVIACADSRVCPSAVLGFQPGDAFTVRNIANLVPPYESGPTETKAALEFSV 171

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             L VENILVIGHS C GIQ+LM MED+  D
Sbjct: 172 NT-----------------------------LNVENILVIGHSRCGGIQALMKMEDEG-D 231

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           S      SF+H W+V  K AK   KA A++L+FD QC+HCEK SIN SL+ L+ YPWIEE
Sbjct: 232 SR-----SFIHNWVVVGKKAKESTKAVASNLHFDHQCQHCEKASINHSLERLLGYPWIEE 291

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
           +++Q SL++HGGYY+F+ CTFEKWT+DY    +    +  G  +K++SVW
Sbjct: 292 KVRQGSLSLHGGYYNFVDCTFEKWTVDYA-ASRGKKKEGSGIAVKDRSVW 300

BLAST of Carg22516 vs. ExPASy Swiss-Prot
Match: Q9C6F5 (Beta carbonic anhydrase 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCA6 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.3e-60
Identity = 130/330 (39.39%), Postives = 185/330 (56.06%), Query Frame = 0

Query: 21  IRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHELEVSRNSLGLAQERSSR 80
           +  T +H+N  L     +G++   + E      +   +N   EL +    + L +E    
Sbjct: 13  VSATSVHQNGCLHKLQQIGSDRFQLGEAKAIRLLPRRTNMVQELGIREEFMDLNRE---- 72

Query: 81  MGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVC 140
                    + +    DEM+ RFL FK+  YL  +E F+ALA  Q+PK MVI C DSRVC
Sbjct: 73  --------TETSYDFLDEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKVMVIGCADSRVC 132

Query: 141 PSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFII 200
           PS +LGFQPGEAFT+RNVAN+V   +NGPTET +ALEFAV T                  
Sbjct: 133 PSYVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTT------------------ 192

Query: 201 SRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVA 260
                      L+VENI+V+GHS+C GI +LMS ++     S     S V +W++N K A
Sbjct: 193 -----------LQVENIIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAA 252

Query: 261 KLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCT 320
           KLR + A++HL+FD+QC++CEKESI  S+ NL+TY WI +R+K+  + +HG YY+   C+
Sbjct: 253 KLRTQLASSHLSFDEQCRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCS 289

Query: 321 FEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
            EKW L    T       ++GF I ++ +W
Sbjct: 313 LEKWRLSSDKT-------NYGFYISDREIW 289

BLAST of Carg22516 vs. ExPASy Swiss-Prot
Match: Q94CE4 (Beta carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=BCA4 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.2e-46
Identity = 103/250 (41.20%), Postives = 145/250 (58.00%), Query Frame = 0

Query: 87  AQVKDADK----VFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPS 146
           A++K+ D       + +K  F  FK   YLKN   F  LA+ Q PKF+V +C DSRVCPS
Sbjct: 58  AELKELDSSNSDAIERIKTGFTQFKTEKYLKNSTLFNHLAKTQTPKFLVFACSDSRVCPS 117

Query: 147 NILGFQPGEAFTVRNVANIVPLW-ENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFIIS 206
           +IL FQPGEAF VRN+AN+VP + +   +   AA+E+AV                     
Sbjct: 118 HILNFQPGEAFVVRNIANMVPPFDQKRHSGVGAAVEYAV--------------------- 177

Query: 207 RLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAK 266
            +H       LKVENILVIGHS C GI+ LMS+EDDA  +       F+  W+     A+
Sbjct: 178 -VH-------LKVENILVIGHSCCGGIKGLMSIEDDAAPTQ----SDFIENWVKIGASAR 237

Query: 267 LRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTF 326
            + K     L++D QC  CEKE++N+SL NL++YP++   + + +LA+ GG+Y+F+  TF
Sbjct: 238 NKIKEEHKDLSYDDQCNKCEKEAVNVSLGNLLSYPFVRAEVVKNTLAIRGGHYNFVKGTF 274

Query: 327 EKWTLDYKNT 332
           + W LD+K T
Sbjct: 298 DLWELDFKTT 274

BLAST of Carg22516 vs. ExPASy Swiss-Prot
Match: P17067 (Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.2e-45
Identity = 115/316 (36.39%), Postives = 169/316 (53.48%), Query Frame = 0

Query: 16  SEMAAIRPTLIHKNPFLVSSFSVGTNSL--SMKEEIDDNHMEVPSNRNHELEVSRNSLGL 75
           S  ++  P+LI   P   SS  + T  L   M +  D+   E+      + E+   +   
Sbjct: 39  SSSSSTFPSLIQDKPVFASSSPIITPVLREEMGKGYDEAIEELQKLLREKTELKATAAEK 98

Query: 76  AQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVIS 135
            ++ ++++G  + +      +  + +K  FL FKK  Y KN   +  LA+ Q+P FMV +
Sbjct: 99  VEQITAQLGTTSSSDGIPKSEASERIKTGFLHFKKEKYDKNPALYGELAKGQSPPFMVFA 158

Query: 136 CVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGP-TETKAALEFAVNTLKVGCFPFFI 195
           C DSRVCPS++L FQPGEAF VRNVAN+VP ++      T AA+E+AV            
Sbjct: 159 CSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQAKYAGTGAAIEYAV------------ 218

Query: 196 QLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHK 255
                     LH       LKV NI+VIGHS+C GI+ L+S   D T S+      F+ +
Sbjct: 219 ----------LH-------LKVSNIVVIGHSACGGIKGLLSFPFDGTYST-----DFIEE 278

Query: 256 WMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGG 315
           W+     AK + KA      F + C HCEKE++N SL NL+TYP++ E L  ++LA+ GG
Sbjct: 279 WVKIGLPAKAKVKAQHGDAPFAELCTHCEKEAVNASLGNLLTYPFVREGLVNKTLALKGG 320

Query: 316 YYDFLSCTFEKWTLDY 329
           YYDF+  +FE W L++
Sbjct: 339 YYDFVKGSFELWGLEF 320

BLAST of Carg22516 vs. ExPASy Swiss-Prot
Match: Q9ZUC2 (Beta carbonic anhydrase 3 OS=Arabidopsis thaliana OX=3702 GN=BCA3 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.7e-44
Identity = 108/286 (37.76%), Postives = 158/286 (55.24%), Query Frame = 0

Query: 58  SNRNHELEVSRNSLGLAQERSSRMGVKNVAQVK---------DADKV--FDEMKMRFLSF 117
           S  ++E  + R  LG    + S +G    A++K         D++K+   + +K  FL F
Sbjct: 2   STESYEDAIKR--LGELLSKKSDLGNVAAAKIKKLTDELEELDSNKLDAVERIKSGFLHF 61

Query: 118 KKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWE 177
           K +NY KN   + +LA+ Q PKF+V +C DSRV PS+IL FQ GEAF VRN+AN+VP ++
Sbjct: 62  KTNNYEKNPTLYNSLAKSQTPKFLVFACADSRVSPSHILNFQLGEAFIVRNIANMVPPYD 121

Query: 178 NGP-TETKAALEFAVNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSC 237
               +   AALE+                              I +L VENILVIGHS C
Sbjct: 122 KTKHSNVGAALEY-----------------------------PITVLNVENILVIGHSCC 181

Query: 238 AGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESI 297
            GI+ LM++ED+   +       F+  W+     AK R K     L+F+ QC +CEKE++
Sbjct: 182 GGIKGLMAIEDNTAPTKT----EFIENWIQICAPAKNRIKQDCKDLSFEDQCTNCEKEAV 241

Query: 298 NLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNT 332
           N+SL NL++YP++ ER+ +  LA+ G +YDF+  TF+ W LD+K T
Sbjct: 242 NVSLGNLLSYPFVRERVVKNKLAIRGAHYDFVKGTFDLWELDFKTT 252

BLAST of Carg22516 vs. ExPASy TrEMBL
Match: A0A6J1GHG7 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111454204 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 4.8e-178
Identity = 316/351 (90.03%), Postives = 318/351 (90.60%), Query Frame = 0

Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NH+EV SNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             LKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NT-----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 322

BLAST of Carg22516 vs. ExPASy TrEMBL
Match: A0A6J1KMD2 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111495926 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.3e-175
Identity = 312/351 (88.89%), Postives = 316/351 (90.03%), Query Frame = 0

Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NHMEVPSNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHMEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
            HELEVSRNSLGLAQERSS MGVKNVAQVKDADKVFDEMKMRFLSFKKH YL+NLEHFQA
Sbjct: 61  YHELEVSRNSLGLAQERSSWMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLENLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             LKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NT-----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWM NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMANAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAG+DDHGFQIKNQS+WC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGNDDHGFQIKNQSLWC 322

BLAST of Carg22516 vs. ExPASy TrEMBL
Match: A0A6J1GHC0 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454204 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.2e-175
Identity = 312/348 (89.66%), Postives = 314/348 (90.23%), Query Frame = 0

Query: 4   LIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHE 63
           LI PRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NH+EV SNRNHE
Sbjct: 46  LIGPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNRNHE 105

Query: 64  LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAE 123
           LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQALAE
Sbjct: 106 LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAE 165

Query: 124 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTL 183
           LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAVNT 
Sbjct: 166 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNT- 225

Query: 184 KVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSL 243
                                       LKVENILVIGHSSCAGIQSLMSMEDDATDSSL
Sbjct: 226 ----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATDSSL 285

Query: 244 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 303
           FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK
Sbjct: 286 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 345

Query: 304 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 346 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 364

BLAST of Carg22516 vs. ExPASy TrEMBL
Match: A0A6J1KHU8 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495926 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.0e-173
Identity = 308/348 (88.51%), Postives = 312/348 (89.66%), Query Frame = 0

Query: 4   LIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHE 63
           LI PRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NHMEVPSNR HE
Sbjct: 34  LIGPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHMEVPSNRYHE 93

Query: 64  LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAE 123
           LEVSRNSLGLAQERSS MGVKNVAQVKDADKVFDEMKMRFLSFKKH YL+NLEHFQALAE
Sbjct: 94  LEVSRNSLGLAQERSSWMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLENLEHFQALAE 153

Query: 124 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTL 183
           LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAVNT 
Sbjct: 154 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNT- 213

Query: 184 KVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSL 243
                                       LKVENILVIGHSSCAGIQSLMSMEDDATDSSL
Sbjct: 214 ----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATDSSL 273

Query: 244 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 303
           FCYGSFVHKWM NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK
Sbjct: 274 FCYGSFVHKWMANAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 333

Query: 304 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAG+DDHGFQIKNQS+WC
Sbjct: 334 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGNDDHGFQIKNQSLWC 352

BLAST of Carg22516 vs. ExPASy TrEMBL
Match: A0A6J1GHD3 (beta carbonic anhydrase 5, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454204 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.4e-169
Identity = 306/348 (87.93%), Postives = 308/348 (88.51%), Query Frame = 0

Query: 4   LIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHE 63
           LI PRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NH+EV SNRNHE
Sbjct: 46  LIGPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNRNHE 105

Query: 64  LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAE 123
           LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQALAE
Sbjct: 106 LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAE 165

Query: 124 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTL 183
           LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAVNT 
Sbjct: 166 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNT- 225

Query: 184 KVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSL 243
                                       LKVENILVIGHSSCAGIQSLMSMEDDATDS  
Sbjct: 226 ----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATDS-- 285

Query: 244 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 303
               SFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK
Sbjct: 286 ----SFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 345

Query: 304 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 346 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 358

BLAST of Carg22516 vs. TAIR 10
Match: AT4G33580.1 (beta carbonic anhydrase 5 )

HSP 1 Score: 278.5 bits (711), Expect = 7.5e-75
Identity = 148/290 (51.03%), Postives = 194/290 (66.90%), Query Frame = 0

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           N ++  S  + GL QE +   GV  + +  + D VFD+MK RFL+FKK  Y+ + EH++ 
Sbjct: 52  NLQVMASGKTPGLTQEAN---GVA-IDRQNNTD-VFDDMKQRFLAFKKLKYMDDFEHYKN 111

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LA+ QAPKF+VI+C DSRVCPS +LGFQPG+AFTVRN+AN+VP +E+GPTETKAALEF+V
Sbjct: 112 LADAQAPKFLVIACADSRVCPSAVLGFQPGDAFTVRNIANLVPPYESGPTETKAALEFSV 171

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             L VENILVIGHS C GIQ+LM MED+  D
Sbjct: 172 NT-----------------------------LNVENILVIGHSRCGGIQALMKMEDEG-D 231

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           S      SF+H W+V  K AK   KA A++L+FD QC+HCEK SIN SL+ L+ YPWIEE
Sbjct: 232 SR-----SFIHNWVVVGKKAKESTKAVASNLHFDHQCQHCEKASINHSLERLLGYPWIEE 291

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
           +++Q SL++HGGYY+F+ CTFEKWT+DY    +    +  G  +K++SVW
Sbjct: 292 KVRQGSLSLHGGYYNFVDCTFEKWTVDYA-ASRGKKKEGSGIAVKDRSVW 300

BLAST of Carg22516 vs. TAIR 10
Match: AT4G33580.2 (beta carbonic anhydrase 5 )

HSP 1 Score: 275.0 bits (702), Expect = 8.3e-74
Identity = 148/291 (50.86%), Postives = 195/291 (67.01%), Query Frame = 0

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLK-NLEHFQ 120
           N ++  S  + GL QE +   GV  + +  + D VFD+MK RFL+FKK  Y++ + EH++
Sbjct: 52  NLQVMASGKTPGLTQEAN---GVA-IDRQNNTD-VFDDMKQRFLAFKKLKYIRDDFEHYK 111

Query: 121 ALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFA 180
            LA+ QAPKF+VI+C DSRVCPS +LGFQPG+AFTVRN+AN+VP +E+GPTETKAALEF+
Sbjct: 112 NLADAQAPKFLVIACADSRVCPSAVLGFQPGDAFTVRNIANLVPPYESGPTETKAALEFS 171

Query: 181 VNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDAT 240
           VNT                             L VENILVIGHS C GIQ+LM MED+  
Sbjct: 172 VNT-----------------------------LNVENILVIGHSRCGGIQALMKMEDEG- 231

Query: 241 DSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIE 300
           DS      SF+H W+V  K AK   KA A++L+FD QC+HCEK SIN SL+ L+ YPWIE
Sbjct: 232 DSR-----SFIHNWVVVGKKAKESTKAVASNLHFDHQCQHCEKASINHSLERLLGYPWIE 291

Query: 301 ERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
           E+++Q SL++HGGYY+F+ CTFEKWT+DY    +    +  G  +K++SVW
Sbjct: 292 EKVRQGSLSLHGGYYNFVDCTFEKWTVDYA-ASRGKKKEGSGIAVKDRSVW 301

BLAST of Carg22516 vs. TAIR 10
Match: AT1G58180.3 (beta carbonic anhydrase 6 )

HSP 1 Score: 234.6 bits (597), Expect = 1.2e-61
Identity = 119/254 (46.85%), Postives = 160/254 (62.99%), Query Frame = 0

Query: 97  DEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVR 156
           DEM+ RFL FK+  YL  +E F+ALA  Q+PK MVI C DSRVCPS +LGFQPGEAFT+R
Sbjct: 26  DEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKVMVIGCADSRVCPSYVLGFQPGEAFTIR 85

Query: 157 NVANIVPLWENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVEN 216
           NVAN+V   +NGPTET +ALEFAV T                             L+VEN
Sbjct: 86  NVANLVTPVQNGPTETNSALEFAVTT-----------------------------LQVEN 145

Query: 217 ILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQ 276
           I+V+GHS+C GI +LMS ++     S     S V +W++N K AKLR + A++HL+FD+Q
Sbjct: 146 IIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAAKLRTQLASSHLSFDEQ 205

Query: 277 CKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAG 336
           C++CEKESI  S+ NL+TY WI +R+K+  + +HG YY+   C+ EKW L    T     
Sbjct: 206 CRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCSLEKWRLSSDKT----- 238

Query: 337 DDDHGFQIKNQSVW 351
             ++GF I ++ +W
Sbjct: 266 --NYGFYISDREIW 238

BLAST of Carg22516 vs. TAIR 10
Match: AT1G58180.2 (beta carbonic anhydrase 6 )

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-61
Identity = 130/330 (39.39%), Postives = 185/330 (56.06%), Query Frame = 0

Query: 21  IRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHELEVSRNSLGLAQERSSR 80
           +  T +H+N  L     +G++   + E      +   +N   EL +    + L +E    
Sbjct: 13  VSATSVHQNGCLHKLQQIGSDRFQLGEAKAIRLLPRRTNMVQELGIREEFMDLNRE---- 72

Query: 81  MGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVC 140
                    + +    DEM+ RFL FK+  YL  +E F+ALA  Q+PK MVI C DSRVC
Sbjct: 73  --------TETSYDFLDEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKVMVIGCADSRVC 132

Query: 141 PSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFII 200
           PS +LGFQPGEAFT+RNVAN+V   +NGPTET +ALEFAV T                  
Sbjct: 133 PSYVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTT------------------ 192

Query: 201 SRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVA 260
                      L+VENI+V+GHS+C GI +LMS ++     S     S V +W++N K A
Sbjct: 193 -----------LQVENIIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAA 252

Query: 261 KLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCT 320
           KLR + A++HL+FD+QC++CEKESI  S+ NL+TY WI +R+K+  + +HG YY+   C+
Sbjct: 253 KLRTQLASSHLSFDEQCRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCS 289

Query: 321 FEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
            EKW L    T       ++GF I ++ +W
Sbjct: 313 LEKWRLSSDKT-------NYGFYISDREIW 289

BLAST of Carg22516 vs. TAIR 10
Match: AT1G58180.4 (beta carbonic anhydrase 6 )

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-61
Identity = 130/330 (39.39%), Postives = 185/330 (56.06%), Query Frame = 0

Query: 21  IRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHELEVSRNSLGLAQERSSR 80
           +  T +H+N  L     +G++   + E      +   +N   EL +    + L +E    
Sbjct: 13  VSATSVHQNGCLHKLQQIGSDRFQLGEAKAIRLLPRRTNMVQELGIREEFMDLNRE---- 72

Query: 81  MGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVC 140
                    + +    DEM+ RFL FK+  YL  +E F+ALA  Q+PK MVI C DSRVC
Sbjct: 73  --------TETSYDFLDEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKVMVIGCADSRVC 132

Query: 141 PSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFII 200
           PS +LGFQPGEAFT+RNVAN+V   +NGPTET +ALEFAV T                  
Sbjct: 133 PSYVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTT------------------ 192

Query: 201 SRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVA 260
                      L+VENI+V+GHS+C GI +LMS ++     S     S V +W++N K A
Sbjct: 193 -----------LQVENIIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAA 252

Query: 261 KLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCT 320
           KLR + A++HL+FD+QC++CEKESI  S+ NL+TY WI +R+K+  + +HG YY+   C+
Sbjct: 253 KLRTQLASSHLSFDEQCRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCS 289

Query: 321 FEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
            EKW L    T       ++GF I ++ +W
Sbjct: 313 LEKWRLSSDKT-------NYGFYISDREIW 289