Carg22387 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22387
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	AAI domain-containing protein
Location	Carg_Chr01: 9286859 .. 9287167 (-)
RNA-Seq Expression	Carg22387
Synteny	Carg22387

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACCACGATGATCAAGTTTTTAGCGTCGTTGCTTTTTAGCCTGTTTCTGGTTGGTACTATTGCCACTCCCTTGTGTCGAGTCGACACGAAGGAGCTCAAGATGTGCCAGCCAGCGGTTGCACCACCACACGGGCAGCTGCTGCAACCACCGACCAAAGGCTGCTGTTCGGTCGTCCGACGTGCAGACCTCAAGTGCCTATGTAGTCTCAAGTCTGTCCTCCCTACCATCGGGATTAACACAGCCAATGCCTTGGCTCTGCCTTCCAAATGTGGCATTCATACTCCCCCCGAGTGCCATGGTTAG

mRNA sequence

ATGGCGACCACGATGATCAAGTTTTTAGCGTCGTTGCTTTTTAGCCTGTTTCTGGTTGGTACTATTGCCACTCCCTTGTGTCGAGTCGACACGAAGGAGCTCAAGATGTGCCAGCCAGCGGTTGCACCACCACACGGGCAGCTGCTGCAACCACCGACCAAAGGCTGCTGTTCGGTCGTCCGACGTGCAGACCTCAAGTGCCTATGTAGTCTCAAGTCTGTCCTCCCTACCATCGGGATTAACACAGCCAATGCCTTGGCTCTGCCTTCCAAATGTGGCATTCATACTCCCCCCGAGTGCCATGGTTAG

Coding sequence (CDS)

ATGGCGACCACGATGATCAAGTTTTTAGCGTCGTTGCTTTTTAGCCTGTTTCTGGTTGGTACTATTGCCACTCCCTTGTGTCGAGTCGACACGAAGGAGCTCAAGATGTGCCAGCCAGCGGTTGCACCACCACACGGGCAGCTGCTGCAACCACCGACCAAAGGCTGCTGTTCGGTCGTCCGACGTGCAGACCTCAAGTGCCTATGTAGTCTCAAGTCTGTCCTCCCTACCATCGGGATTAACACAGCCAATGCCTTGGCTCTGCCTTCCAAATGTGGCATTCATACTCCCCCCGAGTGCCATGGTTAG

Protein sequence

MATTMIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVVRRADLKCLCSLKSVLPTIGINTANALALPSKCGIHTPPECHG

Homology

[Close]

BLAST of Carg22387 vs. NCBI nr
Match: KAG7037350.1 (putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 209.1 bits (531), Expect = 1.7e-50
Identity = 102/102 (100.00%), Postives = 102/102 (100.00%), Query Frame = 0


Query: 1   MATTMIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVV 60
           MATTMIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVV
Sbjct: 1   MATTMIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVV 60

Query: 61  RRADLKCLCSLKSVLPTIGINTANALALPSKCGIHTPPECHG 103
           RRADLKCLCSLKSVLPTIGINTANALALPSKCGIHTPPECHG
Sbjct: 61  RRADLKCLCSLKSVLPTIGINTANALALPSKCGIHTPPECHG 102

[Close]

BLAST of Carg22387 vs. NCBI nr
Match: KAG6607773.1 (putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 196.4 bits (498), Expect = 1.2e-46
Identity = 96/98 (97.96%), Postives = 96/98 (97.96%), Query Frame = 0


Query: 5   MIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVVRRAD 64
           MIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQ LQPPTKGCCSVVRRAD
Sbjct: 1   MIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQPLQPPTKGCCSVVRRAD 60

Query: 65  LKCLCSLKSVLPTIGINTANALALPSKCGIHTPPECHG 103
           LKCLCSLKSVLPTIGINTANALALPSKCGIHTPPEC G
Sbjct: 61  LKCLCSLKSVLPTIGINTANALALPSKCGIHTPPECRG 98

[Close]

BLAST of Carg22387 vs. NCBI nr
Match: XP_038896217.1 (putative lipid-transfer protein DIR1 [Benincasa hispida])

HSP 1 Score: 138.3 bits (347), Expect = 3.8e-29
Identity = 67/96 (69.79%), Postives = 79/96 (82.29%), Query Frame = 0


Query: 8   FLASLLFSLFLVGTIATPLCRVDTKELKM-CQPAVAPPHGQLLQPPTKGCCSVVRRADLK 67
           FL  L  ++ LVG  AT LC+VDTKEL + C+PAVAP HG+ LQPPTK CCSV+R ADLK
Sbjct: 13  FLRLLFLAILLVGISATLLCKVDTKELCVNCRPAVAPLHGKPLQPPTKHCCSVIRHADLK 72

Query: 68  CLCSLKSVLPTIGINTANALALPSKCGIHTPPECHG 103
           CLC+ KS+LP++GINT  ALALPSKCGI+TPPECHG
Sbjct: 73  CLCNFKSILPSVGINTTIALALPSKCGINTPPECHG 108

[Close]

BLAST of Carg22387 vs. NCBI nr
Match: KAA0048160.1 (putative lipid-transfer protein DIR1 [Cucumis melo var. makuwa])

HSP 1 Score: 131.0 bits (328), Expect = 6.0e-27
Identity = 62/85 (72.94%), Postives = 71/85 (83.53%), Query Frame = 0


Query: 16  LFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVVRRADLKCLCSLKSVL 75
           + L GT AT LC+VDTK+L +C+PAVAP HG+ LQPPTK CCSVVR ADLKCLC+LKS+L
Sbjct: 1   MVLAGTSATLLCKVDTKKLCVCRPAVAPLHGKPLQPPTKRCCSVVRSADLKCLCNLKSIL 60

Query: 76  PTIGINTANALALPSKCGIHTPPEC 101
           P+ GIN   ALALPSKCGI TPPEC
Sbjct: 61  PSAGINATIALALPSKCGIRTPPEC 85

[Close]

BLAST of Carg22387 vs. NCBI nr
Match: KAG7037349.1 (putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 127.1 bits (318), Expect = 8.7e-26
Identity = 59/96 (61.46%), Postives = 76/96 (79.17%), Query Frame = 0


Query: 7   KFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAV-APPHGQLLQPPTKGCCSVVRRADL 66
           K L + LF + + G +A+PLC++DT +L +C+PAV  P HGQ L  PT+GCC+VVRRADL
Sbjct: 5   KVLVAALFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADL 64

Query: 67  KCLCSLKSVLPTIGINTANALALPSKCGIHTPPECH 102
           KCLC+LKS+LP IGI+ ANA+ALP KCG+  PPECH
Sbjct: 65  KCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECH 100

[Close]

BLAST of Carg22387 vs. ExPASy TrEMBL
Match: A0A5A7TY68 (Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold63G00600 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.9e-27
Identity = 62/85 (72.94%), Postives = 71/85 (83.53%), Query Frame = 0


Query: 16  LFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVVRRADLKCLCSLKSVL 75
           + L GT AT LC+VDTK+L +C+PAVAP HG+ LQPPTK CCSVVR ADLKCLC+LKS+L
Sbjct: 1   MVLAGTSATLLCKVDTKKLCVCRPAVAPLHGKPLQPPTKRCCSVVRSADLKCLCNLKSIL 60

Query: 76  PTIGINTANALALPSKCGIHTPPEC 101
           P+ GIN   ALALPSKCGI TPPEC
Sbjct: 61  PSAGINATIALALPSKCGIRTPPEC 85

[Close]

BLAST of Carg22387 vs. ExPASy TrEMBL
Match: A0A0A0LBB0 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G759990 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.1e-26
Identity = 63/95 (66.32%), Postives = 74/95 (77.89%), Query Frame = 0


Query: 8   FLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVVRRADLKC 67
           FL  L   + L  T AT LC+VDTKEL +C+PAVAP HG+ LQPPT+ CCSV+R ADLKC
Sbjct: 13  FLRLLFLVMVLAETTATLLCKVDTKELCVCRPAVAPLHGKPLQPPTERCCSVLRSADLKC 72

Query: 68  LCSLKSVLPTIGINTANALALPSKCGIHTPPECHG 103
           LC+LKS+LP+ GIN   ALALPSKCGI +PPEC G
Sbjct: 73  LCNLKSILPSAGINATIALALPSKCGIPSPPECRG 107

[Close]

BLAST of Carg22387 vs. ExPASy TrEMBL
Match: A0A0A0LAS6 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G760500 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.2e-25
Identity = 62/102 (60.78%), Postives = 76/102 (74.51%), Query Frame = 0


Query: 1   MATTMIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPP-HGQLLQPPTKGCCSV 60
           MA + +  L  LL  L      A PLC++DT +LK+C+ AV PP HGQ L  PT+ CCSV
Sbjct: 1   MAPSKLHLLLPLLVLLLPAAVTAYPLCKIDTSDLKLCRSAVTPPDHGQPLPLPTEDCCSV 60

Query: 61  VRRADLKCLCSLKSVLPTIGINTANALALPSKCGIHTPPECH 102
           VR ADLKCLC+LKSVLP++GI+TANALALPSKC + +PPECH
Sbjct: 61  VRHADLKCLCNLKSVLPSMGIDTANALALPSKCNVASPPECH 102

[Close]

BLAST of Carg22387 vs. ExPASy TrEMBL
Match: A0A5A7TX28 (Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold63G00630 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 6.1e-25
Identity = 61/101 (60.40%), Postives = 73/101 (72.28%), Query Frame = 0


Query: 1   MATTMIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPP-HGQLLQPPTKGCCSV 60
           MA + +  L  LL  L      ++PLC +DT +L++C+PAV PP HG+ L  PT  CCSV
Sbjct: 1   MAPSKLHLLLLLLVLLLAGAVTSSPLCEIDTTDLEVCRPAVTPPDHGKTLPLPTDACCSV 60

Query: 61  VRRADLKCLCSLKSVLPTIGINTANALALPSKCGIHTPPEC 101
           VRRADLKCLCSLK  LP+ GINTANALALPSKCG+  PPEC
Sbjct: 61  VRRADLKCLCSLKYALPSFGINTANALALPSKCGVSPPPEC 101

[Close]

BLAST of Carg22387 vs. ExPASy TrEMBL
Match: A0A1S3BHE7 (putative lipid-transfer protein DIR1 OS=Cucumis melo OX=3656 GN=LOC103490109 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 6.1e-25
Identity = 61/101 (60.40%), Postives = 73/101 (72.28%), Query Frame = 0


Query: 1   MATTMIKFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPP-HGQLLQPPTKGCCSV 60
           MA + +  L  LL  L      ++PLC +DT +L++C+PAV PP HG+ L  PT  CCSV
Sbjct: 1   MAPSKLHLLLLLLVLLLAGAVTSSPLCEIDTTDLEVCRPAVTPPDHGKTLPLPTDACCSV 60

Query: 61  VRRADLKCLCSLKSVLPTIGINTANALALPSKCGIHTPPEC 101
           VRRADLKCLCSLK  LP+ GINTANALALPSKCG+  PPEC
Sbjct: 61  VRRADLKCLCSLKYALPSFGINTANALALPSKCGVSPPPEC 101

[Close]

BLAST of Carg22387 vs. TAIR 10
Match: AT5G55450.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 9.9e-12
Identity = 35/90 (38.89%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 9   LASLLFSLFLV-GTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVVRRADLKC 68
           LA +L +  +V    + P+C +DT +L  C+PAV    G    PP   CC+V R A+L+C
Sbjct: 15  LAMVLTAAIMVKEATSIPVCNIDTNDLAKCRPAVT---GNNPPPPGPDCCAVARVANLQC 74

Query: 69  LCSLKSVLPTIGINTANALALPSKCGIHTP 98
           LC  K  LPT+GI+ +    L + CG+++P
Sbjct: 75  LCPYKPYLPTVGIDPSRVRPLLANCGVNSP 101

[Close]

BLAST of Carg22387 vs. TAIR 10
Match: AT5G55410.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 2.9e-11
Identity = 33/96 (34.38%), Postives = 53/96 (55.21%), Query Frame = 0


Query: 8   FLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVVRRADLKC 67
           F   L F++ +    +  +C +D  +++ C+PA+    G    PP   CC VVR+A+ +C
Sbjct: 15  FAMVLTFAMMVKEATSMSICDMDINDMQKCRPAIT---GNNPPPPVNDCCVVVRKANFEC 74

Query: 68  LCSLKSVLPTIGINTANALALPSKCGIHT-PPECHG 103
           LC  K  LP + I+ +  +AL +KCG+ T P  C G
Sbjct: 75  LCRFKFYLPILRIDPSKVVALVAKCGVTTVPRSCQG 107

[Close]

BLAST of Carg22387 vs. TAIR 10
Match: AT5G55410.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 6.4e-11
Identity = 31/91 (34.07%), Postives = 51/91 (56.04%), Query Frame = 0


Query: 8   FLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSVVRRADLKC 67
           F   L F++ +    +  +C +D  +++ C+PA+    G    PP   CC VVR+A+ +C
Sbjct: 15  FAMVLTFAMMVKEATSMSICDMDINDMQKCRPAIT---GNNPPPPVNDCCVVVRKANFEC 74

Query: 68  LCSLKSVLPTIGINTANALALPSKCGIHTPP 99
           LC  K  LP + I+ +  +AL +KCG+ T P
Sbjct: 75  LCRFKFYLPILRIDPSKVVALVAKCGVTTVP 102

[Close]

BLAST of Carg22387 vs. TAIR 10
Match: AT5G55460.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 2.4e-10
Identity = 39/102 (38.24%), Postives = 57/102 (55.88%), Query Frame = 0


Query: 3   TTMIKF--LASLLFSLFLV-GTIATPLCRVDTKELKMCQPAVAPPHGQLLQPPTKGCCSV 62
           T  +KF  LA +L +L L+    +   C ++   L+ C+PAV    G     P K CC +
Sbjct: 6   TRTVKFAALAIVLAALVLMEEPTSITACNINANHLEKCRPAVI---GDNPPSPIKECCEL 65

Query: 63  VRRADLKCLCSLKSVLPTIGINTANALALPSKCGIHT-PPEC 101
           ++ A+LKC+C  KSVLP + +  +   AL SKCG+ T PP C
Sbjct: 66  LQAANLKCICRFKSVLPVLAVYPSKVQALLSKCGLTTIPPAC 104

[Close]

BLAST of Carg22387 vs. TAIR 10
Match: AT3G52130.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 2.4e-05
Identity = 27/66 (40.91%), Postives = 35/66 (53.03%), Query Frame = 0


Query: 33  ELKMCQPAVAPPHGQLLQPPTKGCCSVVRRADLKCLCSLKSVLPTIGINTANALALPSKC 92
           +L  C+ AVAP       PPT+ CCS V      CLC L +  P  GI+ + AL LP +C
Sbjct: 58  QLIPCRAAVAPFSP---IPPTEICCSAVVTLGRPCLCLLANGPPLSGIDRSMALQLPQRC 117

Query: 93  GIHTPP 99
             + PP
Sbjct: 118 SANFPP 120

The following BLAST results are available for this feature:

BLAST of Carg22387 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037350.1	1.7e-50	100.00	putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. arg...	[more]
KAG6607773.1	1.2e-46	97.96	putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_038896217.1	3.8e-29	69.79	putative lipid-transfer protein DIR1 [Benincasa hispida]	[more]
KAA0048160.1	6.0e-27	72.94	putative lipid-transfer protein DIR1 [Cucumis melo var. makuwa]	[more]
KAG7037349.1	8.7e-26	61.46	putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. arg...	[more]

BLAST of Carg22387 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg22387 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TY68	2.9e-27	72.94	Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A0A0LBB0	1.1e-26	66.32	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G759990 PE=4 SV...	[more]
A0A0A0LAS6	1.2e-25	60.78	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G760500 PE=4 SV...	[more]
A0A5A7TX28	6.1e-25	60.40	Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BHE7	6.1e-25	60.40	putative lipid-transfer protein DIR1 OS=Cucumis melo OX=3656 GN=LOC103490109 PE=...	[more]

BLAST of Carg22387 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55450.1	9.9e-12	38.89	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G55410.2	2.9e-11	34.38	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G55410.1	6.4e-11	34.07	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G55460.1	2.4e-10	38.24	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G52130.1	2.4e-05	40.91	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 43..100 e-value: 1.2E-9 score: 38.2
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 24..100 e-value: 3.5E-17 score: 64.0
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 27..99
IPR039265	Putative lipid-transfer protein DIR1-like	PANTHER	PTHR33122	LIPID BINDING PROTEIN-RELATED	coord: 7..101
None	No IPR available	PANTHER	PTHR33122:SF43	LIPID TRANSFER PROTEIN	coord: 7..101
IPR044741	Non-specific lipid-transfer protein-like	CDD	cd04660	nsLTP_like	coord: 27..98 e-value: 6.61789E-16 score: 64.3126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22387-RA	Carg22387-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009627	systemic acquired resistance
molecular_function	GO:0005504	fatty acid binding