Carg21969 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg21969 vs. NCBI nr
Match: KAG6588030.1 (K(+) efflux antiporter 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021928.1 K(+) efflux antiporter 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH 60
           MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH
Sbjct: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH 60

Query: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120
           SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180
           FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240
           IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300
           VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300

Query: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360
           NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS
Sbjct: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360

Query: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480

Query: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540
           FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540

Query: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS
Sbjct: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 587

BLAST of Carg21969 vs. NCBI nr
Match: XP_023531551.1 (K(+) efflux antiporter 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1070.5 bits (2767), Expect = 5.4e-309
Identity = 585/587 (99.66%), Postives = 585/587 (99.66%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH 60
           MRLPSS ILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINAT DSNSSRSANDDH
Sbjct: 1   MRLPSSAILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATFDSNSSRSANDDH 60

Query: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120
           SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180
           FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240
           IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300
           VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300

Query: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360
           NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS
Sbjct: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360

Query: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480

Query: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540
           FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540

Query: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS
Sbjct: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 587

BLAST of Carg21969 vs. NCBI nr
Match: XP_022933892.1 (K(+) efflux antiporter 4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.8e-309
Identity = 587/589 (99.66%), Postives = 587/589 (99.66%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSL--SISILTVTKSDLVFGEINATSDSNSSRSAND 60
           MRLPSSEILVLLLYFHLLLCFATFPSL  SISILTVTKSDLVFGEINATSDSNSSRSAND
Sbjct: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISISILTVTKSDLVFGEINATSDSNSSRSAND 60

Query: 61  DHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKS 120
           DHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKS
Sbjct: 61  DHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKS 120

Query: 121 FQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATC 180
           FQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATC
Sbjct: 121 FQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATC 180

Query: 181 GGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTK 240
           GGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTK
Sbjct: 181 GGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTK 240

Query: 241 LRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERN 300
           LRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERN
Sbjct: 241 LRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERN 300

Query: 301 SINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSI 360
           SINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSI
Sbjct: 301 SINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSI 360

Query: 361 LSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTT 420
           LSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTT
Sbjct: 361 LSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTT 420

Query: 421 DLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVV 480
           DLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVV
Sbjct: 421 DLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVV 480

Query: 481 KGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLF 540
           KGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLF
Sbjct: 481 KGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLF 540

Query: 541 KLIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           KLIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS
Sbjct: 541 KLIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 589

BLAST of Carg21969 vs. NCBI nr
Match: XP_022968974.1 (K(+) efflux antiporter 4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.1e-306
Identity = 581/587 (98.98%), Postives = 581/587 (98.98%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH 60
           MRLPSS ILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATS SNS RSANDDH
Sbjct: 1   MRLPSSAILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSHSNSLRSANDDH 60

Query: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120
           SFADIIDQALEREF ENGQSDQ ADAGSFNNSV DKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFADIIDQALEREFTENGQSDQVADAGSFNNSVTDKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180
           FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240
           IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300
           VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300

Query: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360
           NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS
Sbjct: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360

Query: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480

Query: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540
           FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540

Query: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS
Sbjct: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 587

BLAST of Carg21969 vs. NCBI nr
Match: XP_038879505.1 (K(+) efflux antiporter 4 [Benincasa hispida])

HSP 1 Score: 1006.9 bits (2602), Expect = 7.1e-290
Identity = 548/587 (93.36%), Postives = 569/587 (96.93%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH 60
           MRLPSS ILVL L+FH LLCFATFPSLSIS+LTVTKS+LV GEINAT+DSNSSRSANDDH
Sbjct: 1   MRLPSSAILVLFLFFH-LLCFATFPSLSISLLTVTKSELVTGEINATADSNSSRSANDDH 60

Query: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120
           SFA+IID+ALEREF EN Q+D+ ADAGSFN+SVA+KQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADAGSFNSSVAEKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180
           FHDVF+LDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240
           IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300
           VVRAVAVLGGLLQIFLFMCL GITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNS+
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360
           NA+HGQVTIGTLILQD  VGLLFALLPILGGTSGVLQG+LSMTKSL+VLITF I L+ILS
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLLVLITFLIILTILS 360

Query: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTCVP FLKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVI+IKT+VV  VVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIIIKTVVVTTVVKG 480

Query: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540
           FGYNNKTSLLVGMSLAQIGEF+FVLLSRASNLHLVEGKLY+LLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           IPAVVRIGVL RWFSPDGLSE GFKGDGFRTDG KRITLVVQDSHVS
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDGFRTDGAKRITLVVQDSHVS 586

BLAST of Carg21969 vs. ExPASy Swiss-Prot
Match: Q9ZUN3 (K(+) efflux antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=KEA4 PE=2 SV=2)

HSP 1 Score: 808.9 bits (2088), Expect = 3.7e-233
Identity = 458/583 (78.56%), Postives = 508/583 (87.14%), Query Frame = 0

Query: 6   SEILVLLLYFHLLLCFATFPSLSISILTVTKSD-LVFGEINAT-SDSNSSRSANDDHSFA 65
           S I + LL   L+  F  F S + S  +  ++D +V  EIN T  +SN++ +   + SFA
Sbjct: 12  SVITMRLLTLLLICTFFFFFSFAYSAESDNETDSVVTREINGTVVESNATSAKPREDSFA 71

Query: 66  DIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNE--SKEEKSFQF 125
           D+ID+ALE+EF +N Q ++  D GSFNNSVAD+QAVLETVARVK KKNE  +KEEKSF  
Sbjct: 72  DMIDRALEKEFPDNDQ-NEVPDPGSFNNSVADQQAVLETVARVKPKKNETKTKEEKSF-- 131

Query: 126 HDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGGI 185
              FNLDNEN  ED P LIDRKDNVFI+SNPKSKYPVLQLDLRLISDLVVVIVSATCGGI
Sbjct: 132 ---FNLDNENGVEDTPRLIDRKDNVFIMSNPKSKYPVLQLDLRLISDLVVVIVSATCGGI 191

Query: 186 AFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLRV 245
           AFACAGQPV TGYLLAGS+IGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFS  KLRV
Sbjct: 192 AFACAGQPVITGYLLAGSIIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSAAKLRV 251

Query: 246 VRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSIN 305
           VRAVA+ GGLLQIFLFMCLSGITASLCGGK +EG+FVGAFLSMSSTAVVLKFLMERNSI+
Sbjct: 252 VRAVAIPGGLLQIFLFMCLSGITASLCGGKLTEGIFVGAFLSMSSTAVVLKFLMERNSIS 311

Query: 306 ALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILSR 365
           ALHGQ+T+GTLILQD  VGLLFALLP+LGGTSGVLQG+LSM KSL +LI F   L +LSR
Sbjct: 312 ALHGQITVGTLILQDCAVGLLFALLPVLGGTSGVLQGVLSMAKSLAILIAFLGALFVLSR 371

Query: 366 TCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA 425
           T VPWFLKLM SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA
Sbjct: 372 TWVPWFLKLMTSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA 431

Query: 426 QHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKGF 485
           QHTLEQVEPIRNFFAALFLASIGMLIH+HFLWNH+DILLAAV+LVIVIKT+VVA VVK F
Sbjct: 432 QHTLEQVEPIRNFFAALFLASIGMLIHMHFLWNHVDILLAAVLLVIVIKTVVVAIVVKVF 491

Query: 486 GYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKLI 545
           GYNNKT++LVGMSLAQIGEF+FVLLSRASNLHL+E KLY+LLLGTTALSLVTTPLLFKLI
Sbjct: 492 GYNNKTAVLVGMSLAQIGEFAFVLLSRASNLHLIESKLYLLLLGTTALSLVTTPLLFKLI 551

Query: 546 PAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDS 585
           PAVV +GVL RWFSPD  +E GFKG+ + ++  KRI+L++Q S
Sbjct: 552 PAVVHLGVLLRWFSPDSSTEIGFKGELYHSESAKRISLMIQGS 588

BLAST of Carg21969 vs. ExPASy Swiss-Prot
Match: B5X0N6 (K(+) efflux antiporter 6 OS=Arabidopsis thaliana OX=3702 GN=KEA6 PE=2 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 4.4e-226
Identity = 443/592 (74.83%), Postives = 506/592 (85.47%), Query Frame = 0

Query: 3   LPSSEILVLLLYFHLLLCFATFPSLSIS---IL--TVTKSDLVFGEINATSDSNSSRSAN 62
           L S ++ +LLL     LCF+     +IS   +L  TV  S      +NA+  S+SS    
Sbjct: 12  LSSQQLALLLLLLSFFLCFSVASPRAISDSDLLDETVANSSSSVASLNAS--SSSSLVKP 71

Query: 63  DDHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKS-KKNESKEE 122
            + SFADIID+ALE+EF E+ Q ++ AD GSFNNSVA +QAVLETVARVKS KKNE+KEE
Sbjct: 72  KEGSFADIIDRALEKEFNESDQ-NEVADPGSFNNSVAGQQAVLETVARVKSTKKNETKEE 131

Query: 123 KSFQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSA 182
           K FQ HDVFNL+N+NRAED PTLIDRKDNVFIISN KSKYPVLQLDLRLISDLVVVIVSA
Sbjct: 132 KRFQLHDVFNLNNDNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLVVVIVSA 191

Query: 183 TCGGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFST 242
           TCGGIAFACAGQPV TGYLLAGS+IGPGGL+F+SEMVQVETVAQFGV+FLLFALGLEFST
Sbjct: 192 TCGGIAFACAGQPVITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFALGLEFST 251

Query: 243 TKLRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLME 302
            KL+VVR+VAVLGGLLQI LFM L GIT SLCGGK SEGVFVGAFLSMSSTAVVLKFLME
Sbjct: 252 AKLKVVRSVAVLGGLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVVLKFLME 311

Query: 303 RNSINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFL 362
           +NS N+LHGQVTIG LILQD  VGLLFALLP+L G SG++ G+LS+ K +V+L++F   L
Sbjct: 312 KNSTNSLHGQVTIGILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLLSFLAVL 371

Query: 363 SILSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIS 422
           SILSRTC+PW LKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIS
Sbjct: 372 SILSRTCIPWLLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIS 431

Query: 423 TTDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAA 482
           TTDLA+HTLEQ+EPIRN FAALFLASIGML++VHFLW H+DILLA+VILVI+IKT +V  
Sbjct: 432 TTDLAEHTLEQIEPIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIKTTIVTT 491

Query: 483 VVKGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPL 542
           VVKGFGYNNKT+LLVG+SLAQIGEF+FVLLSRASNLHL+EGKLY+LLLGTTALSLVTTPL
Sbjct: 492 VVKGFGYNNKTALLVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALSLVTTPL 551

Query: 543 LFKLIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTD-GIKRITLVVQDSHVS 588
           +FK+IPAVV +G+L +WFSPD   E   KG+  R++ G +R+ L+ + SH S
Sbjct: 552 VFKMIPAVVHLGILLKWFSPDSTIE---KGEIVRSESGKQRMILMSRQSHSS 597

BLAST of Carg21969 vs. ExPASy Swiss-Prot
Match: Q8VYR9 (K(+) efflux antiporter 5 OS=Arabidopsis thaliana OX=3702 GN=KEA5 PE=2 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 4.2e-184
Identity = 361/516 (69.96%), Postives = 428/516 (82.95%), Query Frame = 0

Query: 51  NSSRSANDDHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARV---K 110
           NS+   N + S A + D+ LE+EF EN  S + +D  SFN+SVAD+QA +ETVA+V   K
Sbjct: 37  NSTAPGNGEGSIAKMFDRVLEKEFSEN-DSPEGSDGASFNSSVADQQAEIETVAKVTHEK 96

Query: 111 SKKNESKEE---KSFQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLR 170
            K+N+++E    + FQ  DVF+L+NE+ ++DM TLID+K+NVF++SN KSKYP+LQ+DLR
Sbjct: 97  GKRNDTQENNGTRPFQLQDVFSLENED-SDDM-TLIDKKNNVFVMSNKKSKYPILQVDLR 156

Query: 171 LISDLVVVIVSATCGGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVI 230
           LISDLVV+IV A  GGI F+C GQPV  GYLLAGS+IGPGGL F+SEMVQVETVAQFGV+
Sbjct: 157 LISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVV 216

Query: 231 FLLFALGLEFSTTKLRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSM 290
           FLLFALGLEFS TKL+VV  VAVLGGLLQI L M L G+TA LCG + SEG+FVGAFLSM
Sbjct: 217 FLLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSM 276

Query: 291 SSTAVVLKFLMERNSINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTK 350
           SSTAVV+KFL+ERNS ++LHGQVTIG LI QD  VGLLFALLP+LGG SG+LQGI+SM K
Sbjct: 277 SSTAVVVKFLVERNSTSSLHGQVTIGILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGK 336

Query: 351 SLVVLITFFIFLSILSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSL 410
            L++L  +    S+L+ + VP FLKLMI LSSQTNELYQLAAVAFCLL AWCSDKLGLSL
Sbjct: 337 LLLILSIYLTVASLLTWSFVPRFLKLMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSL 396

Query: 411 ELGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVI 470
           ELGSF AGVM+STT+ AQHTLEQVEPIRN FAALFL+SIGMLI+VHFLWNH+DILLA+VI
Sbjct: 397 ELGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAALFLSSIGMLINVHFLWNHVDILLASVI 456

Query: 471 LVIVIKTLVVAAVVKGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLL 530
           LVIVIKT + A VVK F YN + S  VG+ LAQIGEF+FVLLSRASNLH++EGK+Y+LLL
Sbjct: 457 LVIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLL 516

Query: 531 GTTALSLVTTPLLFKLIPAVVRIGVLFRWFSPDGLS 561
           GTTALSLVTTPLLFKLIP+ + +GVL RWF  +  S
Sbjct: 517 GTTALSLVTTPLLFKLIPSAMNLGVLLRWFPSENSS 549

BLAST of Carg21969 vs. ExPASy Swiss-Prot
Match: Q6UWJ1 (Transmembrane and coiled-coil domain-containing protein 3 OS=Homo sapiens OX=9606 GN=TMCO3 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 5.2e-41
Identity = 148/498 (29.72%), Postives = 259/498 (52.01%), Query Frame = 0

Query: 65  IIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQFHDV 124
           ++D+ LE       + ++  +  +F+++ + K    E V RV+ ++  SK+         
Sbjct: 179 MLDEILEDVRKAADRLEEEIEEHAFDDNKSVKGVNFEAVLRVEEEEANSKQ--------- 238

Query: 125 FNLDNENRAED--MPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVV-IVSATCGGI 184
            N+      +D  +  LID ++N +I++ P+    + + D   I D+V + ++S  CG +
Sbjct: 239 -NITKREVEDDLGLSMLIDSQNNQYILTKPRDS-TIPRADHHFIKDIVTIGMLSLPCGWL 298

Query: 185 AFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLRV 244
             A  G P   GY++ G L+GP GL+ +  +VQVET+ +FGV F LF +GLEFS  KLR 
Sbjct: 299 CTA-IGLPTMFGYIICGVLLGPSGLNSIKSIVQVETLGEFGVFFTLFLVGLEFSPEKLRK 358

Query: 245 VRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLM------ 304
           V  +++ G      L +    +   L   K ++ VF+   LS+SST +V +FLM      
Sbjct: 359 VWKISLQGPCYMTLLMIAFGLLWGHLLRIKPTQSVFISTCLSLSSTPLVSRFLMGSARGD 418

Query: 305 ERNSINALHGQVTIGTLILQDSTVGLLFALLPIL--GGTSGVLQGILSMTKSLVVLITFF 364
           +   I+  +  V +G L+ QD  +GL  A++P L   G S     ++ + + LV++    
Sbjct: 419 KEGDID--YSTVLLGMLVTQDVQLGLFMAVMPTLIQAGASASSSIVVEVLRILVLIGQIL 478

Query: 365 IFLSILSRTCV--------PWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLE 424
             L+ +   C+        P++ KL +  S    E+  L   AF  L+   ++ L +S+E
Sbjct: 479 FSLAAVFLLCLVIKKYLIGPYYRKLHME-SKGNKEILILGISAFIFLMLTVTELLDVSME 538

Query: 425 LGSFAAGVMISTTD--LAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAV 484
           LG F AG ++S+    + +     +EPIR+F A +F ASIG+ +   F+   + +L+   
Sbjct: 539 LGCFLAGALVSSQGPVVTEEIATSIEPIRDFLAIVFFASIGLHVFPTFVAYELTVLVFLT 598

Query: 485 ILVIVIKTLVVAAVVKGFGYNNKTSL--LVGMSLAQIGEFSFVLLSRASNLHLVEGKLYV 540
           + V+V+K L+ A V+      +   +  +V   LAQ+ EFSFVL SRA    ++  ++Y+
Sbjct: 599 LSVVVMKFLLAALVLSLILPRSSQYIKWIVSAGLAQVSEFSFVLGSRARRAGVISREVYL 658

BLAST of Carg21969 vs. ExPASy Swiss-Prot
Match: Q8BH01 (Transmembrane and coiled-coil domain-containing protein 3 OS=Mus musculus OX=10090 GN=Tmco3 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 2.0e-40
Identity = 148/500 (29.60%), Postives = 262/500 (52.40%), Query Frame = 0

Query: 65  IIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQFHDV 124
           ++D+ LE       + ++  +  +F+++ + K    E V RV+ ++  SK+  +      
Sbjct: 179 MLDEILEDVRKAADRLEEEIEEHAFDDNKSVKGVNFEAVLRVEEEEASSKQNMT------ 238

Query: 125 FNLDNENRAED---MPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVV-IVSATCGG 184
                +   ED   +  LID ++N +I++ P+    + + D   I D+V + ++S  CG 
Sbjct: 239 -----KREVEDGLGLSMLIDSQNNQYILTKPRDS-TIPRADHHFIKDIVTIGMLSLPCGW 298

Query: 185 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 244
           +  A  G P   GY++ G L+GP GL+ +  +VQVET+ +FGV F LF +GLEFS  KLR
Sbjct: 299 LCTA-IGLPTMFGYIICGVLLGPSGLNSIKSIVQVETLGEFGVFFTLFLVGLEFSPEKLR 358

Query: 245 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLM----- 304
            V  +++ G      L +        L   + ++ VF+   LS+SST +V +FL+     
Sbjct: 359 KVWRISLQGPCYMTLLMIAFGLWWGHLLRIRPTQSVFISTCLSLSSTPLVSRFLVGSARG 418

Query: 305 ERNSINALHGQVTIGTLILQDSTVGLLFALLPIL-----GGTSGVLQGILSMT----KSL 364
           ++ + +  +  V +G L++QD  +GL  A++P L     G +S V+  +L +     + L
Sbjct: 419 DKEAGDIDYSTVLLGMLVMQDVQLGLFIAVMPTLIQAGAGASSSVVMEVLRILFLIGQIL 478

Query: 365 VVLITFFIFLSILSRTCV-PWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLE 424
             L   F+   ++    + P++ KL +  S    E+  L   AF  L+   ++ L +S+E
Sbjct: 479 FSLAAVFLLCLVMKTYLIGPYYRKLHLE-SKGNKEILVLGVSAFTFLMLTVTELLDVSME 538

Query: 425 LGSFAAGVMISTTD--LAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDIL---- 484
           LG F AG ++S+    + +  +  +EPIR+F A +F ASIG+ +   F+   + +L    
Sbjct: 539 LGCFLAGALVSSQGHMVTEEIMTYIEPIRDFLAIIFFASIGLHVFPTFVIYELTVLVFLT 598

Query: 485 LAAVILVIVIKTLVVAAVVKGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKL 540
           L+ VI+  V+  LV++ ++       K   +V   LAQ+ EFSFVL SRA    ++  ++
Sbjct: 599 LSVVIMKFVLAVLVLSLILPRSSQYIK--WIVSAGLAQVSEFSFVLGSRARRAGILSREV 658

BLAST of Carg21969 vs. ExPASy TrEMBL
Match: A0A6J1F140 (K(+) efflux antiporter 4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441171 PE=4 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.3e-309
Identity = 587/589 (99.66%), Postives = 587/589 (99.66%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSL--SISILTVTKSDLVFGEINATSDSNSSRSAND 60
           MRLPSSEILVLLLYFHLLLCFATFPSL  SISILTVTKSDLVFGEINATSDSNSSRSAND
Sbjct: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISISILTVTKSDLVFGEINATSDSNSSRSAND 60

Query: 61  DHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKS 120
           DHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKS
Sbjct: 61  DHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKS 120

Query: 121 FQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATC 180
           FQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATC
Sbjct: 121 FQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATC 180

Query: 181 GGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTK 240
           GGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTK
Sbjct: 181 GGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTK 240

Query: 241 LRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERN 300
           LRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERN
Sbjct: 241 LRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERN 300

Query: 301 SINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSI 360
           SINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSI
Sbjct: 301 SINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSI 360

Query: 361 LSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTT 420
           LSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTT
Sbjct: 361 LSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTT 420

Query: 421 DLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVV 480
           DLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVV
Sbjct: 421 DLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVV 480

Query: 481 KGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLF 540
           KGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLF
Sbjct: 481 KGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLF 540

Query: 541 KLIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           KLIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS
Sbjct: 541 KLIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 589

BLAST of Carg21969 vs. ExPASy TrEMBL
Match: A0A6J1I172 (K(+) efflux antiporter 4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468118 PE=4 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 5.3e-307
Identity = 581/587 (98.98%), Postives = 581/587 (98.98%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH 60
           MRLPSS ILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATS SNS RSANDDH
Sbjct: 1   MRLPSSAILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSHSNSLRSANDDH 60

Query: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120
           SFADIIDQALEREF ENGQSDQ ADAGSFNNSV DKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFADIIDQALEREFTENGQSDQVADAGSFNNSVTDKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180
           FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240
           IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300
           VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300

Query: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360
           NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS
Sbjct: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360

Query: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480

Query: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540
           FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540

Query: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS
Sbjct: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 587

BLAST of Carg21969 vs. ExPASy TrEMBL
Match: A0A1S3BQ67 (K(+) efflux antiporter 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492311 PE=4 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 4.2e-288
Identity = 543/587 (92.50%), Postives = 564/587 (96.08%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH 60
           MRL  S ILVL L+FHLLLCFATFPSLSIS++TVTKS+LV GEINAT+DSNSSRSANDDH
Sbjct: 1   MRLSLSTILVLFLFFHLLLCFATFPSLSISLITVTKSELVPGEINATADSNSSRSANDDH 60

Query: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120
           SFA+IID+ALEREF EN Q+D+ AD GSFNNSVA+KQAVLETVARVKSKKNE+KEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADPGSFNNSVAEKQAVLETVARVKSKKNETKEEKSFQ 120

Query: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180
           FHDVF+LDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240
           IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300
           VVRAVAVLGGLLQIFLFMCL GITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNS+
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360
           NA+HGQVTIGTLILQD  VGLLFALLPILGGTSGVLQG+LSMTKSLVVLI F I L+I S
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLIAFLIILTIFS 360

Query: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTCVP FLKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVI+IKT+VV  VVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIIIKTVVVTTVVKG 480

Query: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540
           FGYNNKTSLLVGMSLAQIGEF+FVLLSRASNLHLVEGKLY+LLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           IPAVVRIGVL RWFSPDGLSE GFKGD FRTDG KRITLVVQDSHVS
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDAFRTDGAKRITLVVQDSHVS 587

BLAST of Carg21969 vs. ExPASy TrEMBL
Match: A0A6J1D311 (K(+) efflux antiporter 4 OS=Momordica charantia OX=3673 GN=LOC111016525 PE=4 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.8e-286
Identity = 541/588 (92.01%), Postives = 562/588 (95.58%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILT-VTKSDLVFGEINATSDSNSSRSANDD 60
           MRLPSS ILV   +FHLLLCF TFPS+S+S  T VTKSD V GEINAT+DSNSSRSANDD
Sbjct: 1   MRLPSSAILV---FFHLLLCFDTFPSISVSFSTVVTKSDFVPGEINATADSNSSRSANDD 60

Query: 61  HSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSF 120
           HSFA+IID+ALEREF EN QSD+ ADAG FNNSVA+KQAVLETVARVKSKKNE+KEEKSF
Sbjct: 61  HSFANIIDRALEREFTENEQSDEVADAGGFNNSVAEKQAVLETVARVKSKKNETKEEKSF 120

Query: 121 QFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCG 180
           QFHDVF+LDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCG
Sbjct: 121 QFHDVFHLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCG 180

Query: 181 GIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKL 240
           GIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKL
Sbjct: 181 GIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKL 240

Query: 241 RVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNS 300
           RVVRAVAVLGGLLQIFLFMCL GITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNS
Sbjct: 241 RVVRAVAVLGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNS 300

Query: 301 INALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSIL 360
           +NALHGQVTIGTLILQD  VGLLFALLPILGGTSGVLQG+LSMTKSLVVL+ F + L++L
Sbjct: 301 VNALHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLVAFLVVLTVL 360

Query: 361 SRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTD 420
           SRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTD
Sbjct: 361 SRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTD 420

Query: 421 LAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVK 480
           LAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVI+IKT+VVA VVK
Sbjct: 421 LAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIIIKTVVVATVVK 480

Query: 481 GFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFK 540
           GFGYNNKTSLLVGMSLAQIGEF+FVLLSRASNLHLVEGKLY+LLLGTTALSLVTTPLLFK
Sbjct: 481 GFGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFK 540

Query: 541 LIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           LIPAVVRIGVL RWF PDGLSE GFKGDGFRTDG KRI LVVQ+SHVS
Sbjct: 541 LIPAVVRIGVLLRWFPPDGLSEIGFKGDGFRTDGAKRIALVVQNSHVS 585

BLAST of Carg21969 vs. ExPASy TrEMBL
Match: A0A0A0M1W9 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G586880 PE=4 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 2.3e-286
Identity = 539/587 (91.82%), Postives = 562/587 (95.74%), Query Frame = 0

Query: 1   MRLPSSEILVLLLYFHLLLCFATFPSLSISILTVTKSDLVFGEINATSDSNSSRSANDDH 60
           MRL  S ILVL L+FHLLLCFATFP+LSIS++TVTKS+LV GEINAT+DSNSSRS NDDH
Sbjct: 1   MRLSLSRILVLFLFFHLLLCFATFPTLSISLITVTKSELVPGEINATADSNSSRSDNDDH 60

Query: 61  SFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNESKEEKSFQ 120
           SFA+IID+ALEREF EN Q+D+ AD GSFNNSVA+KQAVLETVARVKSKKNE+KEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADPGSFNNSVAEKQAVLETVARVKSKKNETKEEKSFQ 120

Query: 121 FHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180
           FHDVF+LDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240
           IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSI 300
           VVRAVAVLGGLLQIFLFMCL GITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNS+
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 301 NALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILS 360
           NA+HGQVTIGTLILQD  VGLLFALLPILGGTSGVLQG+LSMTKSLVVLI F I L+I S
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLIAFLIILTIFS 360

Query: 361 RTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTCVP FLKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKG 480
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKT+VV  VVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTTVVKG 480

Query: 481 FGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKL 540
           FGYNNKTSLLVGMSLAQIGEF+FVLLSRASNLHLVEGKLY+LLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 541 IPAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDSHVS 588
           IPAVVRIGVL RWFSPDG SE GFKGD FRTDG KRITLV+QD+HVS
Sbjct: 541 IPAVVRIGVLLRWFSPDGFSEIGFKGDAFRTDGAKRITLVIQDAHVS 587

BLAST of Carg21969 vs. TAIR 10
Match: AT2G19600.1 (K+ efflux antiporter 4 )

HSP 1 Score: 808.9 bits (2088), Expect = 2.7e-234
Identity = 458/583 (78.56%), Postives = 508/583 (87.14%), Query Frame = 0

Query: 6   SEILVLLLYFHLLLCFATFPSLSISILTVTKSD-LVFGEINAT-SDSNSSRSANDDHSFA 65
           S I + LL   L+  F  F S + S  +  ++D +V  EIN T  +SN++ +   + SFA
Sbjct: 12  SVITMRLLTLLLICTFFFFFSFAYSAESDNETDSVVTREINGTVVESNATSAKPREDSFA 71

Query: 66  DIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKSKKNE--SKEEKSFQF 125
           D+ID+ALE+EF +N Q ++  D GSFNNSVAD+QAVLETVARVK KKNE  +KEEKSF  
Sbjct: 72  DMIDRALEKEFPDNDQ-NEVPDPGSFNNSVADQQAVLETVARVKPKKNETKTKEEKSF-- 131

Query: 126 HDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGGI 185
              FNLDNEN  ED P LIDRKDNVFI+SNPKSKYPVLQLDLRLISDLVVVIVSATCGGI
Sbjct: 132 ---FNLDNENGVEDTPRLIDRKDNVFIMSNPKSKYPVLQLDLRLISDLVVVIVSATCGGI 191

Query: 186 AFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLRV 245
           AFACAGQPV TGYLLAGS+IGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFS  KLRV
Sbjct: 192 AFACAGQPVITGYLLAGSIIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSAAKLRV 251

Query: 246 VRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSIN 305
           VRAVA+ GGLLQIFLFMCLSGITASLCGGK +EG+FVGAFLSMSSTAVVLKFLMERNSI+
Sbjct: 252 VRAVAIPGGLLQIFLFMCLSGITASLCGGKLTEGIFVGAFLSMSSTAVVLKFLMERNSIS 311

Query: 306 ALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFLSILSR 365
           ALHGQ+T+GTLILQD  VGLLFALLP+LGGTSGVLQG+LSM KSL +LI F   L +LSR
Sbjct: 312 ALHGQITVGTLILQDCAVGLLFALLPVLGGTSGVLQGVLSMAKSLAILIAFLGALFVLSR 371

Query: 366 TCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA 425
           T VPWFLKLM SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA
Sbjct: 372 TWVPWFLKLMTSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA 431

Query: 426 QHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAAVVKGF 485
           QHTLEQVEPIRNFFAALFLASIGMLIH+HFLWNH+DILLAAV+LVIVIKT+VVA VVK F
Sbjct: 432 QHTLEQVEPIRNFFAALFLASIGMLIHMHFLWNHVDILLAAVLLVIVIKTVVVAIVVKVF 491

Query: 486 GYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPLLFKLI 545
           GYNNKT++LVGMSLAQIGEF+FVLLSRASNLHL+E KLY+LLLGTTALSLVTTPLLFKLI
Sbjct: 492 GYNNKTAVLVGMSLAQIGEFAFVLLSRASNLHLIESKLYLLLLGTTALSLVTTPLLFKLI 551

Query: 546 PAVVRIGVLFRWFSPDGLSETGFKGDGFRTDGIKRITLVVQDS 585
           PAVV +GVL RWFSPD  +E GFKG+ + ++  KRI+L++Q S
Sbjct: 552 PAVVHLGVLLRWFSPDSSTEIGFKGELYHSESAKRISLMIQGS 588

BLAST of Carg21969 vs. TAIR 10
Match: AT5G11800.1 (K+ efflux antiporter 6 )

HSP 1 Score: 785.4 bits (2027), Expect = 3.1e-227
Identity = 443/592 (74.83%), Postives = 506/592 (85.47%), Query Frame = 0

Query: 3   LPSSEILVLLLYFHLLLCFATFPSLSIS---IL--TVTKSDLVFGEINATSDSNSSRSAN 62
           L S ++ +LLL     LCF+     +IS   +L  TV  S      +NA+  S+SS    
Sbjct: 12  LSSQQLALLLLLLSFFLCFSVASPRAISDSDLLDETVANSSSSVASLNAS--SSSSLVKP 71

Query: 63  DDHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARVKS-KKNESKEE 122
            + SFADIID+ALE+EF E+ Q ++ AD GSFNNSVA +QAVLETVARVKS KKNE+KEE
Sbjct: 72  KEGSFADIIDRALEKEFNESDQ-NEVADPGSFNNSVAGQQAVLETVARVKSTKKNETKEE 131

Query: 123 KSFQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSA 182
           K FQ HDVFNL+N+NRAED PTLIDRKDNVFIISN KSKYPVLQLDLRLISDLVVVIVSA
Sbjct: 132 KRFQLHDVFNLNNDNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLVVVIVSA 191

Query: 183 TCGGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFST 242
           TCGGIAFACAGQPV TGYLLAGS+IGPGGL+F+SEMVQVETVAQFGV+FLLFALGLEFST
Sbjct: 192 TCGGIAFACAGQPVITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFALGLEFST 251

Query: 243 TKLRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLME 302
            KL+VVR+VAVLGGLLQI LFM L GIT SLCGGK SEGVFVGAFLSMSSTAVVLKFLME
Sbjct: 252 AKLKVVRSVAVLGGLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVVLKFLME 311

Query: 303 RNSINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTKSLVVLITFFIFL 362
           +NS N+LHGQVTIG LILQD  VGLLFALLP+L G SG++ G+LS+ K +V+L++F   L
Sbjct: 312 KNSTNSLHGQVTIGILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLLSFLAVL 371

Query: 363 SILSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIS 422
           SILSRTC+PW LKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIS
Sbjct: 372 SILSRTCIPWLLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIS 431

Query: 423 TTDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTLVVAA 482
           TTDLA+HTLEQ+EPIRN FAALFLASIGML++VHFLW H+DILLA+VILVI+IKT +V  
Sbjct: 432 TTDLAEHTLEQIEPIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIKTTIVTT 491

Query: 483 VVKGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSLVTTPL 542
           VVKGFGYNNKT+LLVG+SLAQIGEF+FVLLSRASNLHL+EGKLY+LLLGTTALSLVTTPL
Sbjct: 492 VVKGFGYNNKTALLVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALSLVTTPL 551

Query: 543 LFKLIPAVVRIGVLFRWFSPDGLSETGFKGDGFRTD-GIKRITLVVQDSHVS 588
           +FK+IPAVV +G+L +WFSPD   E   KG+  R++ G +R+ L+ + SH S
Sbjct: 552 VFKMIPAVVHLGILLKWFSPDSTIE---KGEIVRSESGKQRMILMSRQSHSS 597

BLAST of Carg21969 vs. TAIR 10
Match: AT5G51710.1 (K+ efflux antiporter 5 )

HSP 1 Score: 646.0 bits (1665), Expect = 3.0e-185
Identity = 361/516 (69.96%), Postives = 428/516 (82.95%), Query Frame = 0

Query: 51  NSSRSANDDHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARV---K 110
           NS+   N + S A + D+ LE+EF EN  S + +D  SFN+SVAD+QA +ETVA+V   K
Sbjct: 37  NSTAPGNGEGSIAKMFDRVLEKEFSEN-DSPEGSDGASFNSSVADQQAEIETVAKVTHEK 96

Query: 111 SKKNESKEE---KSFQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLR 170
            K+N+++E    + FQ  DVF+L+NE+ ++DM TLID+K+NVF++SN KSKYP+LQ+DLR
Sbjct: 97  GKRNDTQENNGTRPFQLQDVFSLENED-SDDM-TLIDKKNNVFVMSNKKSKYPILQVDLR 156

Query: 171 LISDLVVVIVSATCGGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVI 230
           LISDLVV+IV A  GGI F+C GQPV  GYLLAGS+IGPGGL F+SEMVQVETVAQFGV+
Sbjct: 157 LISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVV 216

Query: 231 FLLFALGLEFSTTKLRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSM 290
           FLLFALGLEFS TKL+VV  VAVLGGLLQI L M L G+TA LCG + SEG+FVGAFLSM
Sbjct: 217 FLLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSM 276

Query: 291 SSTAVVLKFLMERNSINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTK 350
           SSTAVV+KFL+ERNS ++LHGQVTIG LI QD  VGLLFALLP+LGG SG+LQGI+SM K
Sbjct: 277 SSTAVVVKFLVERNSTSSLHGQVTIGILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGK 336

Query: 351 SLVVLITFFIFLSILSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSL 410
            L++L  +    S+L+ + VP FLKLMI LSSQTNELYQLAAVAFCLL AWCSDKLGLSL
Sbjct: 337 LLLILSIYLTVASLLTWSFVPRFLKLMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSL 396

Query: 411 ELGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVI 470
           ELGSF AGVM+STT+ AQHTLEQVEPIRN FAALFL+SIGMLI+VHFLWNH+DILLA+VI
Sbjct: 397 ELGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAALFLSSIGMLINVHFLWNHVDILLASVI 456

Query: 471 LVIVIKTLVVAAVVKGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLL 530
           LVIVIKT + A VVK F YN + S  VG+ LAQIGEF+FVLLSRASNLH++EGK+Y+LLL
Sbjct: 457 LVIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLL 516

Query: 531 GTTALSLVTTPLLFKLIPAVVRIGVLFRWFSPDGLS 561
           GTTALSLVTTPLLFKLIP+ + +GVL RWF  +  S
Sbjct: 517 GTTALSLVTTPLLFKLIPSAMNLGVLLRWFPSENSS 549

BLAST of Carg21969 vs. TAIR 10
Match: AT5G51710.2 (K+ efflux antiporter 5 )

HSP 1 Score: 646.0 bits (1665), Expect = 3.0e-185
Identity = 361/516 (69.96%), Postives = 428/516 (82.95%), Query Frame = 0

Query: 51  NSSRSANDDHSFADIIDQALEREFIENGQSDQAADAGSFNNSVADKQAVLETVARV---K 110
           NS+   N + S A + D+ LE+EF EN  S + +D  SFN+SVAD+QA +ETVA+V   K
Sbjct: 37  NSTAPGNGEGSIAKMFDRVLEKEFSEN-DSPEGSDGASFNSSVADQQAEIETVAKVTHEK 96

Query: 111 SKKNESKEE---KSFQFHDVFNLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLR 170
            K+N+++E    + FQ  DVF+L+NE+ ++DM TLID+K+NVF++SN KSKYP+LQ+DLR
Sbjct: 97  GKRNDTQENNGTRPFQLQDVFSLENED-SDDM-TLIDKKNNVFVMSNKKSKYPILQVDLR 156

Query: 171 LISDLVVVIVSATCGGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVI 230
           LISDLVV+IV A  GGI F+C GQPV  GYLLAGS+IGPGGL F+SEMVQVETVAQFGV+
Sbjct: 157 LISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVV 216

Query: 231 FLLFALGLEFSTTKLRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKSSEGVFVGAFLSM 290
           FLLFALGLEFS TKL+VV  VAVLGGLLQI L M L G+TA LCG + SEG+FVGAFLSM
Sbjct: 217 FLLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSM 276

Query: 291 SSTAVVLKFLMERNSINALHGQVTIGTLILQDSTVGLLFALLPILGGTSGVLQGILSMTK 350
           SSTAVV+KFL+ERNS ++LHGQVTIG LI QD  VGLLFALLP+LGG SG+LQGI+SM K
Sbjct: 277 SSTAVVVKFLVERNSTSSLHGQVTIGILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGK 336

Query: 351 SLVVLITFFIFLSILSRTCVPWFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSL 410
            L++L  +    S+L+ + VP FLKLMI LSSQTNELYQLAAVAFCLL AWCSDKLGLSL
Sbjct: 337 LLLILSIYLTVASLLTWSFVPRFLKLMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSL 396

Query: 411 ELGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVI 470
           ELGSF AGVM+STT+ AQHTLEQVEPIRN FAALFL+SIGMLI+VHFLWNH+DILLA+VI
Sbjct: 397 ELGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAALFLSSIGMLINVHFLWNHVDILLASVI 456

Query: 471 LVIVIKTLVVAAVVKGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLL 530
           LVIVIKT + A VVK F YN + S  VG+ LAQIGEF+FVLLSRASNLH++EGK+Y+LLL
Sbjct: 457 LVIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLL 516

Query: 531 GTTALSLVTTPLLFKLIPAVVRIGVLFRWFSPDGLS 561
           GTTALSLVTTPLLFKLIP+ + +GVL RWF  +  S
Sbjct: 517 GTTALSLVTTPLLFKLIPSAMNLGVLLRWFPSENSS 549

BLAST of Carg21969 vs. TAIR 10
Match: AT4G00630.2 (K+ efflux antiporter 2 )

HSP 1 Score: 133.7 bits (335), Expect = 5.0e-31
Identity = 108/366 (29.51%), Postives = 184/366 (50.27%), Query Frame = 0

Query: 187 GQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLRVVRAVA 246
           G PV  GYL AG LIGP GLS +  +   + +A+FGV+FLLF +GLE S  +L  ++   
Sbjct: 592 GSPVL-GYLAAGILIGPYGLSIIRNVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYV 651

Query: 247 VLGGLLQIFLFMCLSG-ITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSINALHG 306
              G  Q+ +   + G IT  + G      + +G  L++SSTAVVL+ L ER    + HG
Sbjct: 652 FGLGSAQVLVTAAVIGLITHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHG 711

Query: 307 QVTIGTLILQDSTVGLLFALLPILG--------GTSGVLQGI-LSMTKSLVVLITFFIFL 366
           + T   L+ QD  V +L  L+P++         G   + + + L+  K+ V +       
Sbjct: 712 RATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGG 771

Query: 367 SILSRTCVPWFLKLMISLSSQ-----TNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAA 426
            +     + W+ +L+  +  Q       E++    +   L  +  + + GLS+ LG+F A
Sbjct: 772 RLFYNALIFWYTQLLRPIYKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLA 831

Query: 427 GVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKT 486
           G++++ T+ +      + P R     LF  ++GM I    L  +  +++  + L++V KT
Sbjct: 832 GLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLANFPLIMGTLGLLLVGKT 891

Query: 487 LVVAAVVKGFGYNNKTSLLVGMSLAQIGEFSFVLLSRASNLHLVEGKLYVLLLGTTALSL 538
           ++V  + K FG +  +++ VG+ LA  GEF+FV    A N  ++  +L  LL     +S+
Sbjct: 892 ILVVIIGKLFGISIISAVRVGLLLAPGGEFAFVAFGEAVNQGIMTPQLSSLLFLVVGISM 951