Carg21927 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg21927 vs. NCBI nr
Match: KAG7021970.1 (Thiol protease aleurain [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 644.0 bits (1660), Expect = 6.6e-181
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0

Query: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60
           MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA
Sbjct: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60

Query: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120
           RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL
Sbjct: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120

Query: 121 GAAQNCSATAKGNHKLTNVVLPESIGEMMALSALLKIKATVVLAGHSVQLELLKRPIGKH 180
           GAAQNCSATAKGNHKLTNVVLPESIGEMMALSALLKIKATVVLAGHSVQLELLKRPIGKH
Sbjct: 121 GAAQNCSATAKGNHKLTNVVLPESIGEMMALSALLKIKATVVLAGHSVQLELLKRPIGKH 180

Query: 181 MERESPCPSSSWWTVLELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQ 240
           MERESPCPSSSWWTVLELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQ
Sbjct: 181 MERESPCPSSSWWTVLELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQ 240

Query: 241 VVKGFRLYSKGVYTSNSCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFK 300
           VVKGFRLYSKGVYTSNSCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFK
Sbjct: 241 VVKGFRLYSKGVYTSNSCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFK 300

Query: 301 MEMGKNMCGVATCASYPIVA 321
           MEMGKNMCGVATCASYPIVA
Sbjct: 301 MEMGKNMCGVATCASYPIVA 320

BLAST of Carg21927 vs. NCBI nr
Match: XP_022933179.1 (thiol protease aleurain-like [Cucurbita moschata])

HSP 1 Score: 472.6 bits (1215), Expect = 2.6e-129
Identity = 259/364 (71.15%), Postives = 269/364 (73.90%), Query Frame = 0

Query: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60
           MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA
Sbjct: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60

Query: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120
           RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL
Sbjct: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120

Query: 121 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 180
           GAAQNCSATAKGNHKLTNVVLPES                  G     S    ++A    
Sbjct: 121 GAAQNCSATAKGNHKLTNVVLPESKDWRDDGIVSPVKDQGHCGSCWTFSTTGALEAAYRQ 180

Query: 181 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 240
           A G  + L                          E +K   G   E   P  + +     
Sbjct: 181 AHGEGISLSEQQLVDCAGAFNNFGCNGGLPSQAFEYIKYNGGLATEAAYPYTAKNGECKY 240

Query: 241 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300
                   +VD      + + T+GAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN
Sbjct: 241 MSENVGVKVVD------SVNITLGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300

Query: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY
Sbjct: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 358

BLAST of Carg21927 vs. NCBI nr
Match: KAG6588084.1 (Thiol protease aleurain-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 472.2 bits (1214), Expect = 3.5e-129
Identity = 259/364 (71.15%), Postives = 269/364 (73.90%), Query Frame = 0

Query: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60
           MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA
Sbjct: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60

Query: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120
           RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL
Sbjct: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120

Query: 121 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 180
           GAAQNCSATAKGNHKLTNVVLPES                  G     S    ++A    
Sbjct: 121 GAAQNCSATAKGNHKLTNVVLPESKDWRDDGIVSPVKDQGHCGSCWTFSTTGALEAAYRQ 180

Query: 181 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 240
           A G  + L                          E +K   G   E   P  + +     
Sbjct: 181 AHGEGISLSEQQLVDCAGAFNNFGCNGGLPSQAFEYIKFNGGLATEAAYPYTAKNGECKY 240

Query: 241 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300
                   +VD      + + T+GAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN
Sbjct: 241 MSENVGVKVVD------SVNITLGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300

Query: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY
Sbjct: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 358

BLAST of Carg21927 vs. NCBI nr
Match: XP_023531660.1 (thiol protease aleurain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 469.5 bits (1207), Expect = 2.2e-128
Identity = 258/364 (70.88%), Postives = 268/364 (73.63%), Query Frame = 0

Query: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60
           MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA
Sbjct: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60

Query: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120
           RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL
Sbjct: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120

Query: 121 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 180
           GAAQNCSATAKGNHKLTNVVLPES                  G     S    ++A    
Sbjct: 121 GAAQNCSATAKGNHKLTNVVLPESKDWRDDGIVSPVKDQGHCGSCWTFSTTGALEAAYRQ 180

Query: 181 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 240
           A G  + L                          E +K   G   E   P  + +     
Sbjct: 181 AHGEGISLSEQQLVDCAGAFNNFGCNGGLPSQAFEYIKYNGGLATEAAYPYTAKNGECKY 240

Query: 241 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300
                   +VD      + + T+GAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN
Sbjct: 241 MSENVGVKVVD------SVNITLGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300

Query: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWG KWGDNGYFKMEMGKNMCGVATCASY
Sbjct: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGSKWGDNGYFKMEMGKNMCGVATCASY 358

BLAST of Carg21927 vs. NCBI nr
Match: XP_022967696.1 (thiol protease aleurain-like [Cucurbita maxima])

HSP 1 Score: 463.8 bits (1192), Expect = 1.2e-126
Identity = 254/364 (69.78%), Postives = 267/364 (73.35%), Query Frame = 0

Query: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60
           MEMA RLLFVSSVLL+LSCAVA SVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA
Sbjct: 1   MEMAHRLLFVSSVLLILSCAVAESVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60

Query: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120
           RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADW+WEEFKKHRL
Sbjct: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWSWEEFKKHRL 120

Query: 121 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 180
           GAAQNCSATAKGNHKLTNVVLPES                  G     S    ++A    
Sbjct: 121 GAAQNCSATAKGNHKLTNVVLPESKDWRDDGIVSPVKDQGHCGSCWTFSTTGALEAAYRQ 180

Query: 181 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 240
           A G  + L                          E +K   G   E   P  + +     
Sbjct: 181 AHGEGISLSEQQLVDCAGAFNNFGCNGGLPSQAFEYIKYNGGLATEAAYPYTAKNGECKY 240

Query: 241 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300
                   ++D      + + T+GAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN
Sbjct: 241 MSENVGVKVID------SVNITLGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300

Query: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY
Sbjct: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 358

BLAST of Carg21927 vs. ExPASy Swiss-Prot
Match: A0A072UTP9 (Pro-cathepsin H OS=Medicago truncatula OX=3880 GN=CP PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-92
Identity = 187/356 (52.53%), Postives = 230/356 (64.61%), Query Frame = 0

Query: 12  SVLLVLSC---AVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFARFAHRYGK 71
           ++L+V  C   A AG  F DSNPIRMVSD    +E ++++V+G +RHA+ FARFA+RYGK
Sbjct: 5   TLLIVFFCVATAAAGLSFHDSNPIRMVSD----MEEQLLQVIGESRHAVSFARFANRYGK 64

Query: 72  RYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGAAQNCSA 131
           RY+T +E+K RF IF E+L+LIKSTN++ L Y LG+NHFADWTWEEF+ HRLGAAQNCSA
Sbjct: 65  RYDTVDEMKRRFKIFSENLQLIKSTNKKRLGYTLGVNHFADWTWEEFRSHRLGAAQNCSA 124

Query: 132 TAKGNHKLTNVVLP-------------------------------------ESIGEMMAL 191
           T KGNH++T+VVLP                                     ++ G+ ++L
Sbjct: 125 TLKGNHRITDVVLPAEKDWRKEGIVSEVKDQGHCGSCWTFSTTGALESAYAQAFGKNISL 184

Query: 192 SALLKIKATVVLAGH-------SVQLELLKRPIGKHMERESPCPSSSWWTVLELLTTLAV 251
           S    +                S   E +K   G   E   P    +    L   T+  V
Sbjct: 185 SEQQLVDCAGAYNNFGCNGGLPSQAFEYIKYNGGLETEEAYPYTGQNG---LCKFTSENV 244

Query: 252 MVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSCGSSPQD 311
            V  L    + + T+GAEDELKHAVAF RPVSVAFQVV  FRLY KGVYTS +CGS+P D
Sbjct: 245 AVQVLG---SVNITLGAEDELKHAVAFARPVSVAFQVVDDFRLYKKGVYTSTTCGSTPMD 304

Query: 312 VNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASYPIVA 321
           VNHAVLAVGYG+EDGVPYWLIKNSWG +WGD+GYFKMEMGKNMCGVATC+SYP+VA
Sbjct: 305 VNHAVLAVGYGIEDGVPYWLIKNSWGGEWGDHGYFKMEMGKNMCGVATCSSYPVVA 350

BLAST of Carg21927 vs. ExPASy Swiss-Prot
Match: Q8H166 (Thiol protease aleurain OS=Arabidopsis thaliana OX=3702 GN=ALEU PE=1 SV=2)

HSP 1 Score: 333.2 bits (853), Expect = 3.3e-90
Identity = 179/352 (50.85%), Postives = 221/352 (62.78%), Query Frame = 0

Query: 13  VLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFARFAHRYGKRYET 72
           V+LV + A A   FD+SNPIRMVSD LRE+E  V +++G +RH L FARF HRYGK+Y+ 
Sbjct: 13  VVLVAASAAANIGFDESNPIRMVSDGLREVEESVSQILGQSRHVLSFARFTHRYGKKYQN 72

Query: 73  AEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGAAQNCSATAKG 132
            EE+KLRF IF E+L+LI+STN++GLSYKLG+N FAD TW+EF++ +LGAAQNCSAT KG
Sbjct: 73  VEEMKLRFSIFKENLDLIRSTNKKGLSYKLGVNQFADLTWQEFQRTKLGAAQNCSATLKG 132

Query: 133 NHKLTNVVLPE-------------------------------------SIGEMMALSALL 192
           +HK+T   LPE                                     + G+ ++LS   
Sbjct: 133 SHKVTEAALPETKDWREDGIVSPVKDQGGCGSCWTFSTTGALEAAYHQAFGKGISLSEQQ 192

Query: 193 KIKATVVLAGH-------SVQLELLKRPIGKHMERESPCPSSSWWTVLELLTTLAVMVDC 252
            +        +       S   E +K   G   E+  P       T       + V V  
Sbjct: 193 LVDCAGAFNNYGCNGGLPSQAFEYIKSNGGLDTEKAYPYTGKD-ETCKFSAENVGVQV-- 252

Query: 253 LPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSCGSSPQDVNHA 312
              L + + T+GAEDELKHAV  VRPVS+AF+V+  FRLY  GVYT + CGS+P DVNHA
Sbjct: 253 ---LNSVNITLGAEDELKHAVGLVRPVSIAFEVIHSFRLYKSGVYTDSHCGSTPMDVNHA 312

Query: 313 VLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASYPIVA 321
           VLAVGYGVEDGVPYWLIKNSWG  WGD GYFKMEMGKNMCG+ATCASYP+VA
Sbjct: 313 VLAVGYGVEDGVPYWLIKNSWGADWGDKGYFKMEMGKNMCGIATCASYPVVA 358

BLAST of Carg21927 vs. ExPASy Swiss-Prot
Match: Q8RWQ9 (Thiol protease aleurain-like OS=Arabidopsis thaliana OX=3702 GN=At3g45310 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 5.6e-90
Identity = 180/364 (49.45%), Postives = 227/364 (62.36%), Query Frame = 0

Query: 3   MAPRLLFVSSVLLVLSCAVAGSV--FDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 62
           M+ +L   SS+LL+L  A A     FD+SNPI+MVSD L ELE  VV+++G +RH L F+
Sbjct: 1   MSVKLNLSSSILLILFAAAASKEIGFDESNPIKMVSDNLHELEDTVVQILGQSRHVLSFS 60

Query: 63  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 122
           RF HRYGK+Y++ EE+KLRF +F E+L+LI+STN++GLSYKL LN FAD TW+EF++++L
Sbjct: 61  RFTHRYGKKYQSVEEMKLRFSVFKENLDLIRSTNKKGLSYKLSLNQFADLTWQEFQRYKL 120

Query: 123 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 182
           GAAQNCSAT KG+HK+T   +P++                  G     S    ++A    
Sbjct: 121 GAAQNCSATLKGSHKITEATVPDTKDWREDGIVSPVKEQGHCGSCWTFSTTGALEAAYHQ 180

Query: 183 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 242
           A G  + L                          E +K   G   E   P          
Sbjct: 181 AFGKGISLSEQQLVDCAGTFNNFGCHGGLPSQAFEYIKYNGGLDTEEAYPYTGKDGGCKF 240

Query: 243 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 302
                   + D      + + T+GAEDELKHAV  VRPVSVAF+VV  FR Y KGV+TSN
Sbjct: 241 SAKNIGVQVRD------SVNITLGAEDELKHAVGLVRPVSVAFEVVHEFRFYKKGVFTSN 300

Query: 303 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           +CG++P DVNHAVLAVGYGVED VPYWLIKNSWG +WGDNGYFKMEMGKNMCGVATC+SY
Sbjct: 301 TCGNTPMDVNHAVLAVGYGVEDDVPYWLIKNSWGGEWGDNGYFKMEMGKNMCGVATCSSY 358

BLAST of Carg21927 vs. ExPASy Swiss-Prot
Match: Q10717 (Cysteine proteinase 2 OS=Zea mays OX=4577 GN=CCP2 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.7e-84
Identity = 185/366 (50.55%), Postives = 221/366 (60.38%), Query Frame = 0

Query: 3   MAPRLLFVSSVLLVL-SCAVAGSVFDDSNPIRMVSDRLRE-LESEVVRVVGHTRHALRFA 62
           M PR LFV +V+++  + AV  S F DSNPIR V+DR    LES V   +G TR ALRFA
Sbjct: 1   MVPRRLFVLAVVVLADTAAVVNSGFADSNPIRPVTDRAASALESTVFAALGRTRDALRFA 60

Query: 63  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 122
           RFA RYGK YE+A E+  RF IF ESL+L++STNR+GLSY+LG+N FAD +WEEF+  RL
Sbjct: 61  RFAVRYGKSYESAAEVHKRFRIFSESLQLVRSTNRKGLSYRLGINRFADMSWEEFRATRL 120

Query: 123 GAAQNCSATAKGNHKL--TNVVLPES-----------------IGEMMALSALLKIKATV 182
           GAAQNCSAT  GNH++    V LPE+                  G     S    ++A  
Sbjct: 121 GAAQNCSATLTGNHRMRAAAVALPETKDWREDGIVSPVKNQGHCGSCWTFSTTGALEAAY 180

Query: 183 VLA-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWT 242
             A G  + L                          E +K   G   E   P    +   
Sbjct: 181 TQATGKPISLSEQQLVDCGFAFNNFGCNGGLPSQAFEYIKYNGGLDTEESYPYQGVNGIC 240

Query: 243 VLELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYT 302
             +       ++D      + + T+GAEDELK AV  VRPVSVAF+V+ GFRLY  GVYT
Sbjct: 241 KFKNENVGVKVLD------SVNITLGAEDELKDAVGLVRPVSVAFEVITGFRLYKSGVYT 300

Query: 303 SNSCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCA 321
           S+ CG++P DVNHAVLAVGYGVEDGVPYWLIKNSWG  WGD GYFKMEMGKNMCGVATCA
Sbjct: 301 SDHCGTTPMDVNHAVLAVGYGVEDGVPYWLIKNSWGADWGDEGYFKMEMGKNMCGVATCA 360

BLAST of Carg21927 vs. ExPASy Swiss-Prot
Match: P25778 (Oryzain gamma chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0442300 PE=2 SV=2)

HSP 1 Score: 309.3 bits (791), Expect = 5.1e-83
Identity = 182/354 (51.41%), Postives = 218/354 (61.58%), Query Frame = 0

Query: 7   LLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRE-LESEVVRVVGHTRHALRFARFAHR 66
           LL  ++V    + A A S FDDSNPIR V+D     LES V+  +G TR ALRFARFA R
Sbjct: 9   LLAAAAVAATSAVAAASSGFDDSNPIRSVTDHAASALESTVIAALGRTRDALRFARFAVR 68

Query: 67  YGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGAAQN 126
           +GKRY  A E++ RF IF ESLEL++STNR+GL Y+LG+N FAD +WEEF+  RLGAAQN
Sbjct: 69  HGKRYGDAAEVQRRFRIFSESLELVRSTNRRGLPYRLGINRFADMSWEEFQASRLGAAQN 128

Query: 127 CSATAKGNHKLTN-VVLPES-----------------IGEMMALSALLKIKATVVLA-GH 186
           CSAT  GNH++ +   LPE+                  G     S    ++A    A G 
Sbjct: 129 CSATLAGNHRMRDAAALPETKDWREDGIVSPVKDQGHCGSCWTFSTTGSLEAAYTQATGK 188

Query: 187 SVQLELLKRPIGKHMERESPC----PSSSWWTVL--------ELLTTLAVMVDCLPK--- 246
            V L   +            C    PS ++  +         E      V   C  K   
Sbjct: 189 PVSLSEQQLVDCATAYNNFGCSGGLPSQAFEYIKYNGGLDTEEAYPYTGVNGICHYKPEN 248

Query: 247 -----LLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSCGSSPQDVN 306
                L + + T+GAEDELK+AV  VRPVSVAFQV+ GFR+Y  GVYTS+ CG+SP DVN
Sbjct: 249 VGVKVLDSVNITLGAEDELKNAVGLVRPVSVAFQVINGFRMYKSGVYTSDHCGTSPMDVN 308

Query: 307 HAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASYPIVA 321
           HAVLAVGYGVE+GVPYWLIKNSWG  WGDNGYFKMEMGKNMCG+ATCASYPIVA
Sbjct: 309 HAVLAVGYGVENGVPYWLIKNSWGADWGDNGYFKMEMGKNMCGIATCASYPIVA 362

BLAST of Carg21927 vs. ExPASy TrEMBL
Match: A0A6J1EZ10 (thiol protease aleurain-like OS=Cucurbita moschata OX=3662 GN=LOC111440035 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.3e-129
Identity = 259/364 (71.15%), Postives = 269/364 (73.90%), Query Frame = 0

Query: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60
           MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA
Sbjct: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60

Query: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120
           RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL
Sbjct: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120

Query: 121 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 180
           GAAQNCSATAKGNHKLTNVVLPES                  G     S    ++A    
Sbjct: 121 GAAQNCSATAKGNHKLTNVVLPESKDWRDDGIVSPVKDQGHCGSCWTFSTTGALEAAYRQ 180

Query: 181 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 240
           A G  + L                          E +K   G   E   P  + +     
Sbjct: 181 AHGEGISLSEQQLVDCAGAFNNFGCNGGLPSQAFEYIKYNGGLATEAAYPYTAKNGECKY 240

Query: 241 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300
                   +VD      + + T+GAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN
Sbjct: 241 MSENVGVKVVD------SVNITLGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300

Query: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY
Sbjct: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 358

BLAST of Carg21927 vs. ExPASy TrEMBL
Match: A0A6J1HSV8 (thiol protease aleurain-like OS=Cucurbita maxima OX=3661 GN=LOC111467145 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.9e-127
Identity = 254/364 (69.78%), Postives = 267/364 (73.35%), Query Frame = 0

Query: 1   MEMAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60
           MEMA RLLFVSSVLL+LSCAVA SVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA
Sbjct: 1   MEMAHRLLFVSSVLLILSCAVAESVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 60

Query: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 120
           RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADW+WEEFKKHRL
Sbjct: 61  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWSWEEFKKHRL 120

Query: 121 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 180
           GAAQNCSATAKGNHKLTNVVLPES                  G     S    ++A    
Sbjct: 121 GAAQNCSATAKGNHKLTNVVLPESKDWRDDGIVSPVKDQGHCGSCWTFSTTGALEAAYRQ 180

Query: 181 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 240
           A G  + L                          E +K   G   E   P  + +     
Sbjct: 181 AHGEGISLSEQQLVDCAGAFNNFGCNGGLPSQAFEYIKYNGGLATEAAYPYTAKNGECKY 240

Query: 241 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300
                   ++D      + + T+GAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN
Sbjct: 241 MSENVGVKVID------SVNITLGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 300

Query: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY
Sbjct: 301 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 358

BLAST of Carg21927 vs. ExPASy TrEMBL
Match: A0A0A0LZK4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G596520 PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 8.9e-115
Identity = 230/357 (64.43%), Postives = 257/357 (71.99%), Query Frame = 0

Query: 3   MAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFARF 62
           MA RL FVSS+LLVLSCAVAGSVFDDSNPIRMVSDRLRELE EVVRV+G   HALRFARF
Sbjct: 1   MASRLFFVSSLLLVLSCAVAGSVFDDSNPIRMVSDRLRELELEVVRVLGQVPHALRFARF 60

Query: 63  AHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGA 122
           AHRYGK+YETAEE+KLRFGIFLESLELIKSTN+QGLSYKLG+N FADWTWEEF+KHRLGA
Sbjct: 61  AHRYGKKYETAEEMKLRFGIFLESLELIKSTNKQGLSYKLGVNQFADWTWEEFRKHRLGA 120

Query: 123 AQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVLAG 182
           AQNCSAT KG+HKLT+  LPES                  G     S    ++A    A 
Sbjct: 121 AQNCSATTKGSHKLTDTALPESKDWRKDGIVSPVKDQGHCGSCWTFSTTGALEAAYAQA- 180

Query: 183 HSVQLELLKRPI---GKHMER---ESPCPSSSWWTVL--------ELLTTLAVMVDC--- 242
           H   + L ++ +   G+           PS ++  +         E      V   C   
Sbjct: 181 HGKGISLSEQQLVDCGRGFNNFGCNGGLPSQAFEYIKYNGGLDTEEAYPYTGVDGSCKFV 240

Query: 243 -----LPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSCGSSPQ 302
                +  + + + T+GAEDELKHAVAFVRPVSVAF+VV GFRLYSKGVYTSNSCGS+P 
Sbjct: 241 PENVGVQVIDSVNITLGAEDELKHAVAFVRPVSVAFEVVSGFRLYSKGVYTSNSCGSTPM 300

Query: 303 DVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASYPIVA 321
           DVNHAVLAVGYGVEDG+PYWLIKNSWG  WGDNGYFKMEMGKNMCGVATCASYPIVA
Sbjct: 301 DVNHAVLAVGYGVEDGIPYWLIKNSWGGNWGDNGYFKMEMGKNMCGVATCASYPIVA 356

BLAST of Carg21927 vs. ExPASy TrEMBL
Match: A0A5A7UIP1 (Thiol protease aleurain-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001590 PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.3e-113
Identity = 230/362 (63.54%), Postives = 250/362 (69.06%), Query Frame = 0

Query: 3   MAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFARF 62
           MA RL FVSSVLL+LSCAVAGSVFDDSNPIRMVSDRLRELE EVVRV+G   HALRFARF
Sbjct: 1   MASRLFFVSSVLLLLSCAVAGSVFDDSNPIRMVSDRLRELELEVVRVLGQVPHALRFARF 60

Query: 63  AHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGA 122
           AHRYGK+YETAEE+K RFGIFLESLELIKSTN+QGLSYKLG+N FADWTWEEFKKHRLGA
Sbjct: 61  AHRYGKKYETAEEMKRRFGIFLESLELIKSTNKQGLSYKLGVNQFADWTWEEFKKHRLGA 120

Query: 123 AQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVLA- 182
           AQNCSAT KG+HKLT+ V PES                  G     S    ++A    A 
Sbjct: 121 AQNCSATTKGSHKLTDAVPPESKDWRKDGIVSPVKDQGHCGSCWTFSTTGALEAAYAQAH 180

Query: 183 GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVLEL 242
           G  V L                          E +K   G   E   P            
Sbjct: 181 GKGVSLSEQQLVDCAGAFNNFGCNGGLPSQAFEYIKYNGGLDTEAAYPYTGKDGKCKFVS 240

Query: 243 LTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSC 302
                 ++D      + + T+GAEDELKHAVAFVRPVSVAF+VV GFRLYSKGVYTSNSC
Sbjct: 241 ENVGVQVID------SVNITLGAEDELKHAVAFVRPVSVAFEVVSGFRLYSKGVYTSNSC 300

Query: 303 GSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASYPI 321
           GS+P DVNHAVLAVGYGVEDG+PYWLIKNSWG  WGD+GYFKMEMGKNMCGVATCASYPI
Sbjct: 301 GSTPMDVNHAVLAVGYGVEDGIPYWLIKNSWGGNWGDDGYFKMEMGKNMCGVATCASYPI 356

BLAST of Carg21927 vs. ExPASy TrEMBL
Match: A0A1S3BPS0 (thiol protease aleurain-like OS=Cucumis melo OX=3656 GN=LOC103492370 PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.3e-113
Identity = 230/362 (63.54%), Postives = 250/362 (69.06%), Query Frame = 0

Query: 3   MAPRLLFVSSVLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFARF 62
           MA RL FVSSVLL+LSCAVAGSVFDDSNPIRMVSDRLRELE EVVRV+G   HALRFARF
Sbjct: 1   MASRLFFVSSVLLLLSCAVAGSVFDDSNPIRMVSDRLRELELEVVRVLGQVPHALRFARF 60

Query: 63  AHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGA 122
           AHRYGK+YETAEE+K RFGIFLESLELIKSTN+QGLSYKLG+N FADWTWEEFKKHRLGA
Sbjct: 61  AHRYGKKYETAEEMKRRFGIFLESLELIKSTNKQGLSYKLGVNQFADWTWEEFKKHRLGA 120

Query: 123 AQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVLA- 182
           AQNCSAT KG+HKLT+ V PES                  G     S    ++A    A 
Sbjct: 121 AQNCSATTKGSHKLTDAVPPESKDWRKDGIVSPVKDQGHCGSCWTFSTTGALEAAYAQAH 180

Query: 183 GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVLEL 242
           G  V L                          E +K   G   E   P            
Sbjct: 181 GKGVSLSEQQLVDCAGAFNNFGCNGGLPSQAFEYIKYNGGLDTEAAYPYTGKDGKCKFVS 240

Query: 243 LTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSC 302
                 ++D      + + T+GAEDELKHAVAFVRPVSVAF+VV GFRLYSKGVYTSNSC
Sbjct: 241 ENVGVQVID------SVNITLGAEDELKHAVAFVRPVSVAFEVVSGFRLYSKGVYTSNSC 300

Query: 303 GSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASYPI 321
           GS+P DVNHAVLAVGYGVEDG+PYWLIKNSWG  WGD+GYFKMEMGKNMCGVATCASYPI
Sbjct: 301 GSTPMDVNHAVLAVGYGVEDGIPYWLIKNSWGGNWGDDGYFKMEMGKNMCGVATCASYPI 356

BLAST of Carg21927 vs. TAIR 10
Match: AT5G60360.1 (aleurain-like protease )

HSP 1 Score: 333.2 bits (853), Expect = 2.3e-91
Identity = 179/352 (50.85%), Postives = 221/352 (62.78%), Query Frame = 0

Query: 13  VLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFARFAHRYGKRYET 72
           V+LV + A A   FD+SNPIRMVSD LRE+E  V +++G +RH L FARF HRYGK+Y+ 
Sbjct: 13  VVLVAASAAANIGFDESNPIRMVSDGLREVEESVSQILGQSRHVLSFARFTHRYGKKYQN 72

Query: 73  AEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGAAQNCSATAKG 132
            EE+KLRF IF E+L+LI+STN++GLSYKLG+N FAD TW+EF++ +LGAAQNCSAT KG
Sbjct: 73  VEEMKLRFSIFKENLDLIRSTNKKGLSYKLGVNQFADLTWQEFQRTKLGAAQNCSATLKG 132

Query: 133 NHKLTNVVLPE-------------------------------------SIGEMMALSALL 192
           +HK+T   LPE                                     + G+ ++LS   
Sbjct: 133 SHKVTEAALPETKDWREDGIVSPVKDQGGCGSCWTFSTTGALEAAYHQAFGKGISLSEQQ 192

Query: 193 KIKATVVLAGH-------SVQLELLKRPIGKHMERESPCPSSSWWTVLELLTTLAVMVDC 252
            +        +       S   E +K   G   E+  P       T       + V V  
Sbjct: 193 LVDCAGAFNNYGCNGGLPSQAFEYIKSNGGLDTEKAYPYTGKD-ETCKFSAENVGVQV-- 252

Query: 253 LPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSCGSSPQDVNHA 312
              L + + T+GAEDELKHAV  VRPVS+AF+V+  FRLY  GVYT + CGS+P DVNHA
Sbjct: 253 ---LNSVNITLGAEDELKHAVGLVRPVSIAFEVIHSFRLYKSGVYTDSHCGSTPMDVNHA 312

Query: 313 VLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASYPIVA 321
           VLAVGYGVEDGVPYWLIKNSWG  WGD GYFKMEMGKNMCG+ATCASYP+VA
Sbjct: 313 VLAVGYGVEDGVPYWLIKNSWGADWGDKGYFKMEMGKNMCGIATCASYPVVA 358

BLAST of Carg21927 vs. TAIR 10
Match: AT3G45310.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 4.0e-91
Identity = 180/364 (49.45%), Postives = 227/364 (62.36%), Query Frame = 0

Query: 3   MAPRLLFVSSVLLVLSCAVAGSV--FDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 62
           M+ +L   SS+LL+L  A A     FD+SNPI+MVSD L ELE  VV+++G +RH L F+
Sbjct: 1   MSVKLNLSSSILLILFAAAASKEIGFDESNPIKMVSDNLHELEDTVVQILGQSRHVLSFS 60

Query: 63  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 122
           RF HRYGK+Y++ EE+KLRF +F E+L+LI+STN++GLSYKL LN FAD TW+EF++++L
Sbjct: 61  RFTHRYGKKYQSVEEMKLRFSVFKENLDLIRSTNKKGLSYKLSLNQFADLTWQEFQRYKL 120

Query: 123 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 182
           GAAQNCSAT KG+HK+T   +P++                  G     S    ++A    
Sbjct: 121 GAAQNCSATLKGSHKITEATVPDTKDWREDGIVSPVKEQGHCGSCWTFSTTGALEAAYHQ 180

Query: 183 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 242
           A G  + L                          E +K   G   E   P          
Sbjct: 181 AFGKGISLSEQQLVDCAGTFNNFGCHGGLPSQAFEYIKYNGGLDTEEAYPYTGKDGGCKF 240

Query: 243 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 302
                   + D      + + T+GAEDELKHAV  VRPVSVAF+VV  FR Y KGV+TSN
Sbjct: 241 SAKNIGVQVRD------SVNITLGAEDELKHAVGLVRPVSVAFEVVHEFRFYKKGVFTSN 300

Query: 303 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           +CG++P DVNHAVLAVGYGVED VPYWLIKNSWG +WGDNGYFKMEMGKNMCGVATC+SY
Sbjct: 301 TCGNTPMDVNHAVLAVGYGVEDDVPYWLIKNSWGGEWGDNGYFKMEMGKNMCGVATCSSY 358

BLAST of Carg21927 vs. TAIR 10
Match: AT5G60360.2 (aleurain-like protease )

HSP 1 Score: 326.6 bits (836), Expect = 2.2e-89
Identity = 178/352 (50.57%), Postives = 220/352 (62.50%), Query Frame = 0

Query: 13  VLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFARFAHRYGKRYET 72
           V+LV + A A   FD+SNPIRMVSD LRE+E  V +++G +RH L FARF HRYGK+Y+ 
Sbjct: 13  VVLVAASAAANIGFDESNPIRMVSDGLREVEESVSQILGQSRHVLSFARFTHRYGKKYQN 72

Query: 73  AEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGAAQNCSATAKG 132
            EE+KLRF IF E+L+LI+STN++GLSYKLG+N FAD TW+EF++ +LGAAQNCSAT KG
Sbjct: 73  VEEMKLRFSIFKENLDLIRSTNKKGLSYKLGVNQFADLTWQEFQRTKLGAAQNCSATLKG 132

Query: 133 NHKLTNVVLPE-------------------------------------SIGEMMALSALL 192
           +HK+T   LPE                                     + G+ ++LS   
Sbjct: 133 SHKVTEAALPETKDWREDGIVSPVKDQGGCGSCWTFSTTGALEAAYHQAFGKGISLSEQQ 192

Query: 193 KIKATVVLAGH-------SVQLELLKRPIGKHMERESPCPSSSWWTVLELLTTLAVMVDC 252
            +        +       S   E +K   G   E+  P       T       + V V  
Sbjct: 193 LVDCAGAFNNYGCNGGLPSQAFEYIKSNGGLDTEKAYPYTGKD-ETCKFSAENVGVQV-- 252

Query: 253 LPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSCGSSPQDVNHA 312
              L + + T+GAEDELKHAV  VRPVS+AF+V+  FRLY  GVYT + CGS+P DVNHA
Sbjct: 253 ---LNSVNITLGAEDELKHAVGLVRPVSIAFEVIHSFRLYKSGVYTDSHCGSTPMDVNHA 312

Query: 313 VLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASYPIVA 321
           VLAVGYGVEDGVPYWLIKNSWG  WGD GYFKMEMGKNMC +ATCASYP+VA
Sbjct: 313 VLAVGYGVEDGVPYWLIKNSWGADWGDKGYFKMEMGKNMC-IATCASYPVVA 357

BLAST of Carg21927 vs. TAIR 10
Match: AT3G45310.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 4.9e-89
Identity = 179/364 (49.18%), Postives = 226/364 (62.09%), Query Frame = 0

Query: 3   MAPRLLFVSSVLLVLSCAVAGSV--FDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFA 62
           M+ +L   SS+LL+L  A A     FD+SNPI+MVSD L ELE  VV+++G +RH L F+
Sbjct: 1   MSVKLNLSSSILLILFAAAASKEIGFDESNPIKMVSDNLHELEDTVVQILGQSRHVLSFS 60

Query: 63  RFAHRYGKRYETAEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRL 122
           RF HRYGK+Y++ EE+KLRF +F E+L+LI+STN++GLSYKL LN FAD TW+EF++++L
Sbjct: 61  RFTHRYGKKYQSVEEMKLRFSVFKENLDLIRSTNKKGLSYKLSLNQFADLTWQEFQRYKL 120

Query: 123 GAAQNCSATAKGNHKLTNVVLPES-----------------IGEMMALSALLKIKATVVL 182
           GAAQNCSAT KG+HK+T   +P++                  G     S    ++A    
Sbjct: 121 GAAQNCSATLKGSHKITEATVPDTKDWREDGIVSPVKEQGHCGSCWTFSTTGALEAAYHQ 180

Query: 183 A-GHSVQL--------------------------ELLKRPIGKHMERESPCPSSSWWTVL 242
           A G  + L                          E +K   G   E   P          
Sbjct: 181 AFGKGISLSEQQLVDCAGTFNNFGCHGGLPSQAFEYIKYNGGLDTEEAYPYTGKDGGCKF 240

Query: 243 ELLTTLAVMVDCLPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSN 302
                   + D      + + T+GAEDELKHAV  VRPVSVAF+VV  FR Y KGV+TSN
Sbjct: 241 SAKNIGVQVRD------SVNITLGAEDELKHAVGLVRPVSVAFEVVHEFRFYKKGVFTSN 300

Query: 303 SCGSSPQDVNHAVLAVGYGVEDGVPYWLIKNSWGPKWGDNGYFKMEMGKNMCGVATCASY 321
           +CG++P DVNHAVLAVGYGVED VPYWLIKNSWG +WGDNGYFKMEMGKNMC VATC+SY
Sbjct: 301 TCGNTPMDVNHAVLAVGYGVEDDVPYWLIKNSWGGEWGDNGYFKMEMGKNMC-VATCSSY 357

BLAST of Carg21927 vs. TAIR 10
Match: AT5G60360.3 (aleurain-like protease )

HSP 1 Score: 312.0 bits (798), Expect = 5.6e-85
Identity = 170/341 (49.85%), Postives = 210/341 (61.58%), Query Frame = 0

Query: 13  VLLVLSCAVAGSVFDDSNPIRMVSDRLRELESEVVRVVGHTRHALRFARFAHRYGKRYET 72
           V+LV + A A   FD+SNPIRMVSD LRE+E  V +++G +RH L FARF HRYGK+Y+ 
Sbjct: 13  VVLVAASAAANIGFDESNPIRMVSDGLREVEESVSQILGQSRHVLSFARFTHRYGKKYQN 72

Query: 73  AEELKLRFGIFLESLELIKSTNRQGLSYKLGLNHFADWTWEEFKKHRLGAAQNCSATAKG 132
            EE+KLRF IF E+L+LI+STN++GLSYKLG+N FAD TW+EF++ +LGAAQNCSAT KG
Sbjct: 73  VEEMKLRFSIFKENLDLIRSTNKKGLSYKLGVNQFADLTWQEFQRTKLGAAQNCSATLKG 132

Query: 133 NHKLTNVVLPE-------------------------------------SIGEMMALSALL 192
           +HK+T   LPE                                     + G+ ++LS   
Sbjct: 133 SHKVTEAALPETKDWREDGIVSPVKDQGGCGSCWTFSTTGALEAAYHQAFGKGISLSEQQ 192

Query: 193 KIKATVVLAGH-------SVQLELLKRPIGKHMERESPCPSSSWWTVLELLTTLAVMVDC 252
            +        +       S   E +K   G   E+  P       T       + V V  
Sbjct: 193 LVDCAGAFNNYGCNGGLPSQAFEYIKSNGGLDTEKAYPYTGKD-ETCKFSAENVGVQV-- 252

Query: 253 LPKLLNTSSTMGAEDELKHAVAFVRPVSVAFQVVKGFRLYSKGVYTSNSCGSSPQDVNHA 310
              L + + T+GAEDELKHAV  VRPVS+AF+V+  FRLY  GVYT + CGS+P DVNHA
Sbjct: 253 ---LNSVNITLGAEDELKHAVGLVRPVSIAFEVIHSFRLYKSGVYTDSHCGSTPMDVNHA 312