Carg21852 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21852
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr05: 253791 .. 254560 (+)
RNA-Seq Expression	Carg21852
Synteny	Carg21852

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATTATCGGGTAGCGAGGTCTTCTTGTAAGGATTCTCTGAAGGTCTTGGAAGAAGATGTAGAACGCGCCAATGCTCTGTTAGTATCCATTTCCCCTTCACTGTTTATCTTCTCTGTTCATAGGAAGTTTGATGTGTTTACTGGTTTCTGTGATGCATTTAGGCCTACGGATATGATTGATTTTGATAGATATTTTACCCAATTCTGGTTTCTGGGCTTGCAGGGCTGCTGCAATTCCAAGAGCCAAACATGGTTCCTATCTCCAAATGAAGCTGGTTTACAATCAATTGGCTCCTCTCCTTCTGTTTTTGATGCAATGGGTGGATTGTTCATGCACTTGTATACTTCCCAGATACTTAAATTTCTTCCACATACTTGTTTACAAGGCTGGTCTCTCTTTTCTTCACTTGTTTTATGTTCGCTGATTTCTTGGAGATGTGAGTTTGTTCTGTCGCATGAACTGTGTCGTTTTCTTTGCTCAGGTTTACACAGATGGAAGGCCTAACATATCAGCTCATGGAAGAAAGGCAACTGTGAAGGAATTTTACGGTACATTTTTCCCTACCAATTGTCTGCCCTGGTTTATCAAAATAGTTCTCTAATGCCTTATGCAAAAACCATTACCATTAGTGCAGTTAAAACGCTTTACATATATCCATGATTATAGAAGGAAGAACATGAGTCAGTGCTATAACACAACTAAAAACAAGAGGGAGATAATTTATACTATGGTTGTTTGCAGGGGGTTTCATTGAAACTTTGTTCTGA

mRNA sequence

ATGTATTATCGGGTAGCGAGGTCTTCTTGTAAGGATTCTCTGAAGGTCTTGGAAGAAGATGTAGAACGCGCCAATGCTCTGGCTGCTGCAATTCCAAGAGCCAAACATGGTTCCTATCTCCAAATGAAGCTGGTTTACACAGATGGAAGGCCTAACATATCAGCTCATGGAAGAAAGGCAACTGTGAAGGAATTTTACGGGGGTTTCATTGAAACTTTGTTCTGA

Coding sequence (CDS)

ATGTATTATCGGGTAGCGAGGTCTTCTTGTAAGGATTCTCTGAAGGTCTTGGAAGAAGATGTAGAACGCGCCAATGCTCTGGCTGCTGCAATTCCAAGAGCCAAACATGGTTCCTATCTCCAAATGAAGCTGGTTTACACAGATGGAAGGCCTAACATATCAGCTCATGGAAGAAAGGCAACTGTGAAGGAATTTTACGGGGGTTTCATTGAAACTTTGTTCTGA

Protein sequence

MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYTDGRPNISAHGRKATVKEFYGGFIETLF

Homology

[Close]

BLAST of Carg21852 vs. NCBI nr
Match: KAG7029236.1 (hypothetical protein SDJN02_07574, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 151.4 bits (381), Expect = 3.1e-33
Identity = 74/74 (100.00%), Postives = 74/74 (100.00%), Query Frame = 0


Query: 1  MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYTDGRPNISAHGRKA 60
          MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYTDGRPNISAHGRKA
Sbjct: 1  MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYTDGRPNISAHGRKA 60

Query: 61 TVKEFYGGFIETLF 75
          TVKEFYGGFIETLF
Sbjct: 61 TVKEFYGGFIETLF 74

[Close]

BLAST of Carg21852 vs. NCBI nr
Match: XP_022962105.1 (E3 ubiquitin-protein ligase CHFR-like isoform X1 [Cucurbita moschata] >XP_022962107.1 E3 ubiquitin-protein ligase CHFR-like isoform X1 [Cucurbita moschata] >KAG6598257.1 E3 ubiquitin-protein ligase AIRP2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 117.5 bits (293), Expect = 5.0e-23
Identity = 67/110 (60.91%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL                
Sbjct: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYNQLAPLLLFLMQWV 60

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPNISAHGRKATVKEFY   + +L
Sbjct: 61  DCSCTCILPRYLNFFHILVYKVYTDGRPNISAHGRKATVKEFYAIILPSL 110

[Close]

BLAST of Carg21852 vs. NCBI nr
Match: XP_022962108.1 (uncharacterized protein LOC111462660 isoform X2 [Cucurbita moschata])

HSP 1 Score: 117.5 bits (293), Expect = 5.0e-23
Identity = 67/110 (60.91%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL                
Sbjct: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYNQLAPLLLFLMQWV 60

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPNISAHGRKATVKEFY   + +L
Sbjct: 61  DCSCTCILPRYLNFFHILVYKVYTDGRPNISAHGRKATVKEFYAIILPSL 110

[Close]

BLAST of Carg21852 vs. NCBI nr
Match: XP_022996708.1 (uncharacterized protein LOC111491874 [Cucurbita maxima] >XP_022996710.1 uncharacterized protein LOC111491874 [Cucurbita maxima])

HSP 1 Score: 115.9 bits (289), Expect = 1.5e-22
Identity = 65/110 (59.09%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYYRVARSSCKDSLKVLEED+ERANALAAAIPRAKHGSYLQMKL                
Sbjct: 1   MYYRVARSSCKDSLKVLEEDIERANALAAAIPRAKHGSYLQMKLVYNQLAPLLLFLMQWV 60

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPNISAHGRKATVK+FY   + +L
Sbjct: 61  DCSCTCVLPRYLNFFHILVYKVYTDGRPNISAHGRKATVKDFYAIILPSL 110

[Close]

BLAST of Carg21852 vs. NCBI nr
Match: XP_023546493.1 (E3 ubiquitin-protein ligase CHFR-like [Cucurbita pepo subsp. pepo] >XP_023546495.1 E3 ubiquitin-protein ligase CHFR-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 114.4 bits (285), Expect = 4.2e-22
Identity = 66/110 (60.00%), Postives = 68/110 (61.82%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYY VARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL                
Sbjct: 1   MYYWVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYNQLAPLLLFLMQWV 60

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPNISAHGRKATVKEFY   + +L
Sbjct: 61  DCSCTCILPRYLNFFHILVYKVYTDGRPNISAHGRKATVKEFYAIILPSL 110

[Close]

BLAST of Carg21852 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.3e-05
Identity = 30/93 (32.26%), Postives = 40/93 (43.01%), Query Frame = 0


Query: 7  RSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------------- 66
          R S KDSLK LE D++ AN LA+  P    G Y+QM+L                      
Sbjct: 2  RKSFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAG 61

[Close]

BLAST of Carg21852 vs. ExPASy TrEMBL
Match: A0A6J1HBV1 (E3 ubiquitin-protein ligase CHFR-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462660 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.4e-23
Identity = 67/110 (60.91%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL                
Sbjct: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYNQLAPLLLFLMQWV 60

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPNISAHGRKATVKEFY   + +L
Sbjct: 61  DCSCTCILPRYLNFFHILVYKVYTDGRPNISAHGRKATVKEFYAIILPSL 110

[Close]

BLAST of Carg21852 vs. ExPASy TrEMBL
Match: A0A6J1HDV0 (uncharacterized protein LOC111462660 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462660 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.4e-23
Identity = 67/110 (60.91%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL                
Sbjct: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYNQLAPLLLFLMQWV 60

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPNISAHGRKATVKEFY   + +L
Sbjct: 61  DCSCTCILPRYLNFFHILVYKVYTDGRPNISAHGRKATVKEFYAIILPSL 110

[Close]

BLAST of Carg21852 vs. ExPASy TrEMBL
Match: A0A6J1KBT4 (uncharacterized protein LOC111491874 OS=Cucurbita maxima OX=3661 GN=LOC111491874 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 7.0e-23
Identity = 65/110 (59.09%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYYRVARSSCKDSLKVLEED+ERANALAAAIPRAKHGSYLQMKL                
Sbjct: 1   MYYRVARSSCKDSLKVLEEDIERANALAAAIPRAKHGSYLQMKLVYNQLAPLLLFLMQWV 60

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPNISAHGRKATVK+FY   + +L
Sbjct: 61  DCSCTCVLPRYLNFFHILVYKVYTDGRPNISAHGRKATVKDFYAIILPSL 110

[Close]

BLAST of Carg21852 vs. ExPASy TrEMBL
Match: A0A6J1KIH2 (uncharacterized protein LOC111496061 OS=Cucurbita maxima OX=3661 GN=LOC111496061 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 8.6e-21
Identity = 60/110 (54.55%), Postives = 67/110 (60.91%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYYR++RSSCKDSLKVLEEDV RANALA AIPRAKHGSYLQMKL                
Sbjct: 111 MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLLLFLMQWV 170

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPN+SAHGRKAT+K+FY   + +L
Sbjct: 171 DCSCTCVLPRYLNFFHILVYKVYTDGRPNLSAHGRKATLKDFYAVILPSL 220

[Close]

BLAST of Carg21852 vs. ExPASy TrEMBL
Match: A0A6J1GGU1 (uncharacterized protein LOC111454098 OS=Cucurbita moschata OX=3662 GN=LOC111454098 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 8.6e-21
Identity = 60/110 (54.55%), Postives = 67/110 (60.91%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------- 60
           MYYR++RSSCKDSLKVLEEDV RANALA AIPRAKHGSYLQMKL                
Sbjct: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60

Query: 61  ---------------------VYTDGRPNISAHGRKATVKEFYGGFIETL 74
                                VYTDGRPN+SAHGRKAT+K+FY   + +L
Sbjct: 61  DCSCTCVLPRYLNFFHILVYKVYTDGRPNLSAHGRKATLKDFYAVILPSL 110

[Close]

BLAST of Carg21852 vs. TAIR 10
Match: AT1G24440.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 5.0e-13
Identity = 43/108 (39.81%), Postives = 55/108 (50.93%), Query Frame = 0


Query: 1   MYYRVARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYT------------- 60
           MYY++ +SS +DSLK+LE D+E AN LAA IP  K G  LQMKLV +             
Sbjct: 1   MYYQLTKSSYRDSLKILEADIEHANGLAAEIPMGKSGVRLQMKLVCSNLAPFFIFLLQWM 60

Query: 61  ----------------------DGRPNISAHGRKATVKEFYGGFIETL 74
                                 DGR N S +GRKAT++EFYG  + +L
Sbjct: 61  DFSCLLPRYFDFFHILIYKVRADGRWNRSRYGRKATIREFYGVILPSL 108

[Close]

BLAST of Carg21852 vs. TAIR 10
Match: AT1G13195.1 (RING/U-box superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 4.0e-10
Identity = 38/103 (36.89%), Postives = 50/103 (48.54%), Query Frame = 0


Query: 8   SSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVY--------------------- 67
           SS  +SLKVLE DV+ AN+LA AIP  K+   LQMKLV+                     
Sbjct: 14  SSYYESLKVLEADVQHANSLAEAIPMGKNNVRLQMKLVHSNFASLLLFLLRWIDLSSSCL 73

Query: 68  ----------------TDGRPNISAHGRKATVKEFYGGFIETL 74
                           +DG+P ++ HGRKAT+ EFYG  + +L
Sbjct: 74  IPRYLNLFHVLVYKVQSDGQPKLTTHGRKATISEFYGVILPSL 116

[Close]

BLAST of Carg21852 vs. TAIR 10
Match: AT3G47160.1 (RING/U-box superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 7.0e-07
Identity = 31/102 (30.39%), Postives = 46/102 (45.10%), Query Frame = 0


Query: 5   VARSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKLVYT----------------- 64
           +A+ S KDSLK LE D++ AN +A   PR K G+ +QM+L YT                 
Sbjct: 1   MAKVSFKDSLKALEADIQHANTVALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHL 60

Query: 65  ----------------DGRPNISAHGRKATVKEFYGGFIETL 74
                           DG+  +S + RK ++K+FY     +L
Sbjct: 61  AGTLGLLRVLIYMTYADGKTTMSVYERKTSIKDFYAVIFPSL 102

[Close]

BLAST of Carg21852 vs. TAIR 10
Match: AT5G01520.2 (RING/U-box superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 9.1e-07
Identity = 30/93 (32.26%), Postives = 40/93 (43.01%), Query Frame = 0


Query: 7  RSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------------- 66
          R S KDSLK LE D++ AN LA+  P    G Y+QM+L                      
Sbjct: 2  RKSFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAG 61

[Close]

BLAST of Carg21852 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 9.1e-07
Identity = 30/93 (32.26%), Postives = 40/93 (43.01%), Query Frame = 0


Query: 7  RSSCKDSLKVLEEDVERANALAAAIPRAKHGSYLQMKL---------------------- 66
          R S KDSLK LE D++ AN LA+  P    G Y+QM+L                      
Sbjct: 2  RKSFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAG 61

The following BLAST results are available for this feature:

BLAST of Carg21852 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029236.1	3.1e-33	100.00	hypothetical protein SDJN02_07574, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022962105.1	5.0e-23	60.91	E3 ubiquitin-protein ligase CHFR-like isoform X1 [Cucurbita moschata] >XP_022962...	[more]
XP_022962108.1	5.0e-23	60.91	uncharacterized protein LOC111462660 isoform X2 [Cucurbita moschata]	[more]
XP_022996708.1	1.5e-22	59.09	uncharacterized protein LOC111491874 [Cucurbita maxima] >XP_022996710.1 uncharac...	[more]
XP_023546493.1	4.2e-22	60.00	E3 ubiquitin-protein ligase CHFR-like [Cucurbita pepo subsp. pepo] >XP_023546495...	[more]

BLAST of Carg21852 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M022	1.3e-05	32.26	E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ...	[more]

BLAST of Carg21852 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBV1	2.4e-23	60.91	E3 ubiquitin-protein ligase CHFR-like isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1HDV0	2.4e-23	60.91	uncharacterized protein LOC111462660 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1KBT4	7.0e-23	59.09	uncharacterized protein LOC111491874 OS=Cucurbita maxima OX=3661 GN=LOC111491874...	[more]
A0A6J1KIH2	8.6e-21	54.55	uncharacterized protein LOC111496061 OS=Cucurbita maxima OX=3661 GN=LOC111496061...	[more]
A0A6J1GGU1	8.6e-21	54.55	uncharacterized protein LOC111454098 OS=Cucurbita moschata OX=3662 GN=LOC1114540...	[more]

BLAST of Carg21852 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24440.1	5.0e-13	39.81	RING/U-box superfamily protein	[more]
AT1G13195.1	4.0e-10	36.89	RING/U-box superfamily protein	[more]
AT3G47160.1	7.0e-07	30.39	RING/U-box superfamily protein	[more]
AT5G01520.2	9.1e-07	32.26	RING/U-box superfamily protein	[more]
AT5G01520.1	9.1e-07	32.26	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR15315:SF35	F21J9.10	coord: 45..68 coord: 4..47
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 45..68 coord: 4..47

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21852-RA	Carg21852-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding