Homology
BLAST of Carg21795 vs. NCBI nr
Match:
KAG7024285.1 (Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1009.6 bits (2609), Expect = 9.3e-291
Identity = 498/498 (100.00%), Postives = 498/498 (100.00%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH
Sbjct: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
Query: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL
Sbjct: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
Query: 241 QSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
QSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE
Sbjct: 241 QSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
Query: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY
Sbjct: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
Query: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGW 420
PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGW
Sbjct: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGW 420
Query: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP
Sbjct: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
Query: 481 LHALCRPRSKVAHLLPQI 499
LHALCRPRSKVAHLLPQI
Sbjct: 481 LHALCRPRSKVAHLLPQI 498
BLAST of Carg21795 vs. NCBI nr
Match:
XP_022936848.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])
HSP 1 Score: 1006.1 bits (2600), Expect = 1.0e-289
Identity = 496/498 (99.60%), Postives = 497/498 (99.80%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH
Sbjct: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
Query: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL
Sbjct: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
Query: 241 QSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
QSKRDAWMQSLIEEHRKQS KDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE
Sbjct: 241 QSKRDAWMQSLIEEHRKQSKKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
Query: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY
Sbjct: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
Query: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGW 420
PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWV+PEKFNPGRFGSEGEGW
Sbjct: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFNPGRFGSEGEGW 420
Query: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP
Sbjct: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
Query: 481 LHALCRPRSKVAHLLPQI 499
LHALCRPRSKVAHLLPQI
Sbjct: 481 LHALCRPRSKVAHLLPQI 498
BLAST of Carg21795 vs. NCBI nr
Match:
XP_023535659.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 994.6 bits (2570), Expect = 3.1e-286
Identity = 490/498 (98.39%), Postives = 493/498 (99.00%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
MAI FLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH
Sbjct: 1 MAITFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRR+SSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNT+NDVVDMRTEFFELMFNIMMR
Sbjct: 121 WRNLRRVSSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTENDVVDMRTEFFELMFNIMMR 180
Query: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL
Sbjct: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
Query: 241 QSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
QSKRDAWMQSLIEEHRKQS KDAKKTMIEVLLSLQQKEP YYKDEYIRGLMLVLLLAGTE
Sbjct: 241 QSKRDAWMQSLIEEHRKQSKKDAKKTMIEVLLSLQQKEPRYYKDEYIRGLMLVLLLAGTE 300
Query: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
GSINTMEWLLSLLLNNPECLQRAQ EIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY
Sbjct: 301 GSINTMEWLLSLLLNNPECLQRAQLEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
Query: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGW 420
PPGPLLIPHESS DCHVGGFRVPAGTTLFVNVWAIQNDPTVWV+PEKFNPGRFGSEGEGW
Sbjct: 361 PPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFNPGRFGSEGEGW 420
Query: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP
Sbjct: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
Query: 481 LHALCRPRSKVAHLLPQI 499
LHALCRPRSKVAHLLPQI
Sbjct: 481 LHALCRPRSKVAHLLPQI 498
BLAST of Carg21795 vs. NCBI nr
Match:
XP_022977008.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])
HSP 1 Score: 994.2 bits (2569), Expect = 4.1e-286
Identity = 489/498 (98.19%), Postives = 492/498 (98.80%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
MAI FLCLLIL ILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTL DISRRH
Sbjct: 1 MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDM+TEFFELMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180
Query: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
MIAGKRYYGED AESEEAERFREIQEETFRLSSKTNLGDFLR+VKWAGLSKGLENRMKEL
Sbjct: 181 MIAGKRYYGEDVAESEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKEL 240
Query: 241 QSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
QSKRDAWMQSLIEEHRKQS KDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE
Sbjct: 241 QSKRDAWMQSLIEEHRKQSKKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
Query: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY
Sbjct: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
Query: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGW 420
PPGPLLIPHESS DCHVGGFRVPAGTTLFVNVWAIQNDPTVWV+PEKFNPGRFGSEGEGW
Sbjct: 361 PPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFNPGRFGSEGEGW 420
Query: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP
Sbjct: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
Query: 481 LHALCRPRSKVAHLLPQI 499
LHALCRPRSKVAHLLPQI
Sbjct: 481 LHALCRPRSKVAHLLPQI 498
BLAST of Carg21795 vs. NCBI nr
Match:
XP_038899302.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 890.2 bits (2299), Expect = 8.3e-255
Identity = 446/506 (88.14%), Postives = 464/506 (91.70%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
MAI FL LL+L IL IFTN FLHRIRNLPPSP+ SLPIIGQLYLLK PLHRTLS+I+ RH
Sbjct: 5 MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPILFF FGFR VLVVSSPSAAE+CLSKND+VFANRP L GKYLGYNYTSLLWAPYGEH
Sbjct: 65 GPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 124
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRISSLEI S HRLQTLSSIRVDEVRTLIRRLYN KNDVVDMRTEFFELMFNIMMR
Sbjct: 125 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMR 184
Query: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240
MIAGKRYYGED AA++EE RFREIQEETFRLSSKTNLGDFLR+VKW GLSKGLEN+++E
Sbjct: 185 MIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRE 244
Query: 241 LQSKRDAWMQSLIEEHRKQSN-------KDAKKTMIEVLLSLQQKEPHYYKDEYIRGLML 300
LQ KRD WMQSLIEEHRKQ N DAKKTMIEVLLSLQQ EPHYYKDE+IRGLML
Sbjct: 245 LQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRGLML 304
Query: 301 VLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSL 360
VLLLAGTEGSINTMEWLLSLLLN+P+CLQRAQ EIDDVVGRSNRILEESDLAHLPYLRSL
Sbjct: 305 VLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYLRSL 364
Query: 361 IHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGR 420
IHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWVEP FNP R
Sbjct: 365 IHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFNPER 424
Query: 421 FGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVG 480
FGSEGEG FKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCF+WESVGGEC+DMREG G
Sbjct: 425 FGSEGEG--FKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGGG 484
Query: 481 LTLPKAVPLHALCRPRSKVAHLLPQI 499
LTLPKA+PLHALCRPRS VAHLLPQI
Sbjct: 485 LTLPKALPLHALCRPRSNVAHLLPQI 508
BLAST of Carg21795 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 481.1 bits (1237), Expect = 1.5e-134
Identity = 249/484 (51.45%), Postives = 334/484 (69.01%), Query Frame = 0
Query: 9 LILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRHGPILFFWF 68
++LL + + R RNLPPSP +LP+IG L+L+ + LHR+L D+S+++G +
Sbjct: 13 VVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQL 72
Query: 69 GFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEHWRNLRRIS 128
G R VLVVSSP+AAEEC +KNDIVFANRP I GKY+GYNYT+++ +PYGEHWRNLRR++
Sbjct: 73 GNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLA 132
Query: 129 SLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKND---VVDMRTEFFELMFNIMMRMIAGK 188
++EIFS L SIR DEV+ L+ LY + V+M+++ EL FN+ MRM+AGK
Sbjct: 133 AVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGK 192
Query: 189 RYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKELQSKRD 248
RY+G+D +S+EA+ FR + E F + +N GDF+ ++W K E ++ ++ + D
Sbjct: 193 RYFGQD-VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF-KNYEKKVSKISQEMD 252
Query: 249 AWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTEGSINT 308
A++Q LI E R N TMI+ LLSLQ+ +P YY D+ I+G+++VLLLAGT+ S T
Sbjct: 253 AFLQRLIHESRINKN---NVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVT 312
Query: 309 MEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMYPPGPL 368
+EW +SLLLN+PE L++A+TEI+ VG SNR++EE DL L YL ++I ET R+ P P+
Sbjct: 313 VEWAMSLLLNHPETLEKARTEIETQVG-SNRLIEEQDLPKLTYLHNIISETFRLCPAAPM 372
Query: 369 LIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGWFFKWM 428
L+PHESS+DC V G+ VP GT L VN WAI DP W EP F P R G E K M
Sbjct: 373 LVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGV-ELEPSKLM 432
Query: 429 PFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVPLHALC 488
PFG GRR CPG GL RVVGL +G+LIQCFEW+ +G IDM EG GLT+PKA PL ALC
Sbjct: 433 PFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALC 489
Query: 489 RPRS 490
+PR+
Sbjct: 493 KPRN 489
BLAST of Carg21795 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 450.7 bits (1158), Expect = 2.2e-125
Identity = 235/494 (47.57%), Postives = 328/494 (66.40%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
+++I +++ L +F N + +NLPP P LPIIG L+ LK+PLH T +S+++
Sbjct: 6 LSLIISLFFLIITLKVFFNT-SRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKY 65
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
G I WFG R V+VVSS + A+EC +KNDIV ANRPH + GKY+GYN T++ +PYG+H
Sbjct: 66 GQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDH 125
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRL----YNTKNDVVDMRTEFFELMFN 180
WRNLRRI S+EI S+HRL + IR DE+ LI++L YN + V++R F E+ FN
Sbjct: 126 WRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE-VELRPMFSEMTFN 185
Query: 181 IMMRMIAGKRYYGE--DAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLE 240
+MRM++GKRYYG D ++ EEA FR I +E L N+GDFL ++W GLE
Sbjct: 186 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GLE 245
Query: 241 NRMKELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVL 300
R+K++ + DA++Q LI+EHR K TMI+ LL+ QQ +P YY D+ I+GLM+V+
Sbjct: 246 KRLKKISKRTDAFLQGLIDEHR--FGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 305
Query: 301 LLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIH 360
LLAGT+ S T+EW +S LLN+PE +++A+ E+D +G +R ++E D++ LPYL+S+++
Sbjct: 306 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGH-DRQVDEHDISKLPYLQSIVY 365
Query: 361 ETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFG 420
ETLR++ PLL+PH SSED +GG+ +P T L VN W I DP +W +P F P RF
Sbjct: 366 ETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFE 425
Query: 421 SEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLT 480
EGE K + FG GRR CPGE L R GL +G LIQCFEW+ +G E IDM E G+T
Sbjct: 426 KEGE--VNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGIT 485
Query: 481 LPKAVPLHALCRPR 489
K L+A+C+ R
Sbjct: 486 AGKKTSLNAMCKVR 490
BLAST of Carg21795 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 448.0 bits (1151), Expect = 1.4e-124
Identity = 226/501 (45.11%), Postives = 334/501 (66.67%), Query Frame = 0
Query: 2 AIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRHG 61
++ +L L + + I +F +NLPP P SLPIIG L+ LKRPLHRT +S ++G
Sbjct: 10 SVFYLALFFIFNIVIRARKF----KNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYG 69
Query: 62 PILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEHW 121
+ WFG R V+VVSS S ++C +KND+V ANRP + GKY+ YNYT+L YGEHW
Sbjct: 70 HVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHW 129
Query: 122 RNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRL---YNTKNDVVDMRTEFFELMFNIM 181
RNLRRI++L++ S HR+ + S IR DE + LI RL +T +++ + +++ FN +
Sbjct: 130 RNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNI 189
Query: 182 MRMIAGKRYYGE--DAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENR 241
MRMI+GKRYYGE D ++ +EA +FR++ E +LS N DF+ ++++ + LE R
Sbjct: 190 MRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKR 249
Query: 242 MKELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLL 301
+K++ K DA+++ LIEEHR + K+ TMI+ LL+LQ +P YY D+ I+GL L +LL
Sbjct: 250 LKDISGKTDAFLRGLIEEHR--TKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLL 309
Query: 302 AGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHET 361
AGT+ S T+EW +S LLN+PE L++ + E+D VG+ +R+++ESDL L YL+++I+ET
Sbjct: 310 AGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQ-DRLVDESDLPKLTYLKNVINET 369
Query: 362 LRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSE 421
LR+Y P PLL+PH +S++C++GG++VP T + +N WAI DP +W E F P RF +
Sbjct: 370 LRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKK 429
Query: 422 GEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLP 481
GE K + FG GRR CPGEGL +R + + + LIQCF+W+ + G+ ID+ E G TL
Sbjct: 430 GE--LEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLT 489
Query: 482 KAVPLHALCRPRSKVAHLLPQ 498
K VPL A+C+ R + + Q
Sbjct: 490 KLVPLKAMCKSRPVINKVFKQ 499
BLAST of Carg21795 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 446.0 bits (1146), Expect = 5.4e-124
Identity = 240/495 (48.48%), Postives = 332/495 (67.07%), Query Frame = 0
Query: 4 IFLCLLILLILHIFTNQFLH-RIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRR--- 63
+ L + LI I + +FL + +NLPPSP LPIIG L LLK P+HRTL S
Sbjct: 8 VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67
Query: 64 --HGPILFFWFGFRPVLVVSSPS-AAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAP 123
G ++ G R V VVSS AAEEC KND+V ANRP +I GK++GYN T+++ AP
Sbjct: 68 NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127
Query: 124 YGEHWRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLI---RRLYNTKNDVVDMRTEFFEL 183
YG+HWRNLRR+ ++EIFSTHRL +R DEVR LI RL TK VV+++ +L
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 187
Query: 184 MFNIMMRMIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGL 243
FN +MRM+ GKRYYGE+ + EEA+R R++ + +S N D++ I++ L
Sbjct: 188 TFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILR---LFSSY 247
Query: 244 ENRMKELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLV 303
ENR+K+L + D ++Q LI++ R Q ++ TMI+ LL LQ+ + YY D+ I+G++L+
Sbjct: 248 ENRVKKLGEETDKFLQGLIDDKRGQ--QETGTTMIDHLLVLQKSDIEYYTDQIIKGIILI 307
Query: 304 LLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLI 363
+++AGT S T+EW LS LLN+P+ + +A+ EID+ VG +R++EE+DL+ LPYL++++
Sbjct: 308 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVG-LDRLIEEADLSELPYLKNIV 367
Query: 364 HETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRF 423
ETLR++P PLL+PH +SEDC +G + +P GTTL VN WAI DP W +P+ F P RF
Sbjct: 368 LETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF 427
Query: 424 GSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGL 483
E E K + FG GRR CPG GL R+VGLA+GSLIQCFEWE VG +DM+EGVG
Sbjct: 428 EKEEEAQ--KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 487
Query: 484 TLPKAVPLHALCRPR 489
T+PKA+PL A+C+ R
Sbjct: 488 TVPKAIPLKAICKAR 494
BLAST of Carg21795 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 439.5 bits (1129), Expect = 5.1e-122
Identity = 231/492 (46.95%), Postives = 317/492 (64.43%), Query Frame = 0
Query: 6 LCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRHGPILF 65
L L ++ + I R++NLPP P ++PIIG L+ LK PLHRT + +S+ +G I
Sbjct: 8 LSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 67
Query: 66 FWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEHWRNLR 125
WFG R V+VVSSPS A EC +KNDI+ ANRP + GKY+ YNYT+L A YG+HWRNLR
Sbjct: 68 LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 127
Query: 126 RISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYN---------TKNDVVDMRTEFFELMFN 185
RI+++++ S +RL + +R DE LI++L TK V++R E+ FN
Sbjct: 128 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTK---VELRPRLTEMTFN 187
Query: 186 IMMRMIAGKRYYGE--DAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLE 245
MMRMI+GKRYYG+ D ++ EEA++FREI E L N GDFL +++ L LE
Sbjct: 188 AMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD-NLE 247
Query: 246 NRMKELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVL 305
R K + + +A+++ LIEEHR+ + TMI+ LL L + +P YY D I+GL+ +
Sbjct: 248 KRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGM 307
Query: 306 LLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIH 365
LLAGT+ S T+EW++S LLN+PE L++A+ E+D +G+ N++++E DL+ LPYL+++I
Sbjct: 308 LLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGK-NKLVDEQDLSKLPYLQNIIS 367
Query: 366 ETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFG 425
ETLR++PP PLL+PH SSEDC +G F VP T + NVW I DP W + F P RF
Sbjct: 368 ETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFE 427
Query: 426 SEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLT 485
E E K M FG GRR CPG L R VG VG LIQCFEWE E +DM EG G+T
Sbjct: 428 KEEE--VNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGIT 487
Query: 486 LPKAVPLHALCR 487
+P +PL A+C+
Sbjct: 488 MPMKIPLRAMCK 491
BLAST of Carg21795 vs. ExPASy TrEMBL
Match:
A0A6J1FED9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=3 SV=1)
HSP 1 Score: 1006.1 bits (2600), Expect = 5.0e-290
Identity = 496/498 (99.60%), Postives = 497/498 (99.80%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH
Sbjct: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
Query: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL
Sbjct: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
Query: 241 QSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
QSKRDAWMQSLIEEHRKQS KDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE
Sbjct: 241 QSKRDAWMQSLIEEHRKQSKKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
Query: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY
Sbjct: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
Query: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGW 420
PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWV+PEKFNPGRFGSEGEGW
Sbjct: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFNPGRFGSEGEGW 420
Query: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP
Sbjct: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
Query: 481 LHALCRPRSKVAHLLPQI 499
LHALCRPRSKVAHLLPQI
Sbjct: 481 LHALCRPRSKVAHLLPQI 498
BLAST of Carg21795 vs. ExPASy TrEMBL
Match:
A0A6J1INS3 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 SV=1)
HSP 1 Score: 994.2 bits (2569), Expect = 2.0e-286
Identity = 489/498 (98.19%), Postives = 492/498 (98.80%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
MAI FLCLLIL ILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTL DISRRH
Sbjct: 1 MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDM+TEFFELMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180
Query: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
MIAGKRYYGED AESEEAERFREIQEETFRLSSKTNLGDFLR+VKWAGLSKGLENRMKEL
Sbjct: 181 MIAGKRYYGEDVAESEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKEL 240
Query: 241 QSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
QSKRDAWMQSLIEEHRKQS KDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE
Sbjct: 241 QSKRDAWMQSLIEEHRKQSKKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLAGTE 300
Query: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY
Sbjct: 301 GSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETLRMY 360
Query: 361 PPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEGEGW 420
PPGPLLIPHESS DCHVGGFRVPAGTTLFVNVWAIQNDPTVWV+PEKFNPGRFGSEGEGW
Sbjct: 361 PPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFNPGRFGSEGEGW 420
Query: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP
Sbjct: 421 FFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPKAVP 480
Query: 481 LHALCRPRSKVAHLLPQI 499
LHALCRPRSKVAHLLPQI
Sbjct: 481 LHALCRPRSKVAHLLPQI 498
BLAST of Carg21795 vs. ExPASy TrEMBL
Match:
A0A0A0L0F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1)
HSP 1 Score: 860.9 bits (2223), Expect = 2.6e-246
Identity = 431/509 (84.68%), Postives = 453/509 (89.00%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1 MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61 GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
Query: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240
MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
Query: 241 LQSKRDAWMQSLIEEHRKQSNK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 300
LQ KRD WMQSLIEEHRK+ N DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360
LMLVLLLAGTEGSINTMEWLLSLLLN+P LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFN 420
RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWVEP KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
Query: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480
P RFG +GEG FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
Query: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 499
G GLTLPKA+PL LCRPRS HLL QI
Sbjct: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
BLAST of Carg21795 vs. ExPASy TrEMBL
Match:
A0A1S3BVF3 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1)
HSP 1 Score: 845.1 bits (2182), Expect = 1.5e-241
Identity = 422/510 (82.75%), Postives = 450/510 (88.24%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
M I FL LLIL IL IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 31 MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPILFF FGFRPVLVVSSPSA E+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 91 GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRISSLEI S+HRLQTLSSIRVDEVR LIRRLYN +NDVVDMRTEFFELMFNIMMR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210
Query: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240
MIAGKRYYGE A+SEE RF+EIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270
Query: 241 LQSKRDAWMQSLIEEHRKQSNK-----------DAKKTMIEVLLSLQQKEPHYYKDEYIR 300
LQ +RD WMQSLIEEHRK+ + DAKKTMIEVLLSLQQ EP YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330
Query: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPY 360
GLMLVLLLAGTEGSINTMEWLLSLLLN+P L+RAQ EID+VVG++NR+LEESDL HLPY
Sbjct: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390
Query: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKF 420
LRSLIHETLRMYPPGPLLIPHESS+DCHVGGF VPAGT LFVNVWAIQNDPTVWVEPEKF
Sbjct: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450
Query: 421 NPGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMR 480
NP RF S+GEG FKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCFEWES+GG+CIDM
Sbjct: 451 NPDRFKSDGEG--FKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMS 510
Query: 481 EGVGLTLPKAVPLHALCRPRSKVAHLLPQI 499
EG GLTLPKA+PL LCRPRS HLL QI
Sbjct: 511 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 538
BLAST of Carg21795 vs. ExPASy TrEMBL
Match:
A0A7J0EHR3 (Cytochrome P450 superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_04g0007630 PE=3 SV=1)
HSP 1 Score: 640.6 bits (1651), Expect = 5.5e-180
Identity = 324/503 (64.41%), Postives = 393/503 (78.13%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
MA +++ + ++L +I FLH+IRNLPPSPF +LPI G LYLLK P+HRTLS+IS H
Sbjct: 1 MATLYVYIPLILAFYIIAKHFLHKIRNLPPSPFPALPIFGHLYLLKTPIHRTLSNISAHH 60
Query: 61 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120
GPIL FGFRPVL+VSSPSAAEECLSKNDIVFANRP L+ GK+LG NY +L WAPYG
Sbjct: 61 GPILLLRFGFRPVLLVSSPSAAEECLSKNDIVFANRPRLLAGKHLGQNYNTLSWAPYGAR 120
Query: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180
WRNLRRI+++EI S+HRLQTLSSIR DEVR+LIRRL ++ VDM+ FFELM N+ MR
Sbjct: 121 WRNLRRIAAVEILSSHRLQTLSSIRADEVRSLIRRLAR-GSEGVDMKAAFFELMLNVTMR 180
Query: 181 MIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKEL 240
MIAGK YYGED AE+E A RFREI ETF L+ +N GDFL ++KW G S GLE R+ L
Sbjct: 181 MIAGKTYYGEDVAEAEAARRFREIVTETFVLAGASNFGDFLPVMKWIGAS-GLEKRLIAL 240
Query: 241 QSKRDAWMQSLIEEHRKQ----SNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLL 300
+ KRD +MQ +IEE R++ +++ KKTM+EVLLSLQ+ EP +Y D+ IRG+M VLL
Sbjct: 241 REKRDEFMQEMIEEQRRRIGGGGSEEKKKTMVEVLLSLQETEPEHYTDQMIRGMMSVLLA 300
Query: 301 AGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHET 360
AGT+ S+ TMEW LSLLLN P LQ+AQTEID VG+ +R+++ESD+A LPYLR +I+ET
Sbjct: 301 AGTDTSVGTMEWALSLLLNQPYALQKAQTEIDKQVGQ-HRLIDESDIADLPYLRCIINET 360
Query: 361 LRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRF-GS 420
+RMYP GPLL+PHESSEDC VGGF +P GT L VN+WAIQNDP +WVEP KF P RF G
Sbjct: 361 MRMYPAGPLLVPHESSEDCMVGGFHIPRGTMLLVNMWAIQNDPKIWVEPNKFKPERFEGL 420
Query: 421 EGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTL 480
EG FK++PFG+GRR CPGEGL LR+VGLA+GSLIQCF+WE VG E +DM EG GLTL
Sbjct: 421 EGARDGFKFVPFGSGRRVCPGEGLALRMVGLALGSLIQCFDWERVGKEMVDMTEGTGLTL 480
Query: 481 PKAVPLHALCRPRSKVAHLLPQI 499
PKA PL A CRPR + ++L QI
Sbjct: 481 PKARPLMAKCRPRLTMVNILSQI 500
BLAST of Carg21795 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 449.5 bits (1155), Expect = 3.5e-126
Identity = 240/501 (47.90%), Postives = 325/501 (64.87%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
M I C ++L + IF + R NLPPSP ++LP+IG L LLK PLHR +S+
Sbjct: 5 MLIFTFCFIVLSL--IFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSL 64
Query: 61 G--PILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYG 120
G PI+ G R + VVSS S AEEC +KND++ ANR I K++ Y ++++ A Y
Sbjct: 65 GDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYS 124
Query: 121 EHWRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKN---DVVDMRTEFFELMF 180
EHWRNLRRI +LEIFS HRL + SSIR DE+R LI RL + V+M++ F +L F
Sbjct: 125 EHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTF 184
Query: 181 NIMMRMIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLEN 240
N ++RM+AGK YYG+ + EA+R R + E S N D++ I+ W S E
Sbjct: 185 NNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS---ET 244
Query: 241 RMKELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLL 300
R+K+L + D ++Q L++E R+ K + TM++ LL LQ+ +P YY D I+G ML L+
Sbjct: 245 RIKKLAGRLDEFLQGLVDEKREGKEK-KENTMVDHLLCLQETQPEYYMDRIIKGTMLSLI 304
Query: 301 LAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHE 360
GT+ + T+EW LS LLNNPE L +A+ EID ++G +R+LEESD+ +LPYL++++ E
Sbjct: 305 AGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIG-VDRLLEESDIPNLPYLQNIVSE 364
Query: 361 TLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGS 420
TLR+YP P+L+PH +S+DC VGG+ +P GT L N WAI DP +W +P F P RF
Sbjct: 365 TLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK 424
Query: 421 EGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTL 480
EGE K MPFG GRR CPG GL R+V L++GSLIQCFEWE +G E +DM EG GLT+
Sbjct: 425 EGEA--KKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTM 484
Query: 481 PKAVPLHALCRPRSKVAHLLP 497
PKA PL A+CR R V +LP
Sbjct: 485 PKARPLEAMCRARDFVGKILP 496
BLAST of Carg21795 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 449.5 bits (1155), Expect = 3.5e-126
Identity = 238/496 (47.98%), Postives = 333/496 (67.14%), Query Frame = 0
Query: 1 MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60
++ +FL + I L+L +R NLPPSP + LP+IG L+LLK+P+HRT IS+
Sbjct: 9 LSFLFLTISIKLLL-----TKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSL 68
Query: 61 G--PILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYG 120
G PI G R V V+SS S AEEC +KND+V ANRP +I K++GYN+T+++ A YG
Sbjct: 69 GNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYG 128
Query: 121 EHWRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKND---VVDMRTEFFELMF 180
+HWRNLRRI+++EIFS+HR+ T SSIR DE+R LI L V++++ L F
Sbjct: 129 DHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAF 188
Query: 181 NIMMRMIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLEN 240
N ++ M+AGKRYYG +++EA+ RE+ E + NL D+L + W EN
Sbjct: 189 NNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFEN 248
Query: 241 RMKELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLL 300
+ K L ++ D +Q L++E K++ K+ +T+I+ LLS Q+ EP YY D I+G++L L+
Sbjct: 249 QTKILGNRLDRVLQKLVDE--KRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALV 308
Query: 301 LAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHE 360
LAGT+ S T+EW +S LLN+PE L++A+ EIDD +G S+R++EESD+ +L YL++++ E
Sbjct: 309 LAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIG-SDRLVEESDIVNLHYLQNIVSE 368
Query: 361 TLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGS 420
TLR+YP PLL+PH SS++C V G+ +P T L NVWA+ DP +W EPE+F P RF
Sbjct: 369 TLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEK 428
Query: 421 EGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTL 480
EGE K MPFG GRR CPG LG R+V LA+G LIQ FEWE VG E +DM EG G+T+
Sbjct: 429 EGEA--RKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITM 488
Query: 481 PKAVPLHALCRPRSKV 492
PKA PL A+C+ R+ V
Sbjct: 489 PKATPLRAMCKARAIV 491
BLAST of Carg21795 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 446.0 bits (1146), Expect = 3.8e-125
Identity = 240/495 (48.48%), Postives = 332/495 (67.07%), Query Frame = 0
Query: 4 IFLCLLILLILHIFTNQFLH-RIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRR--- 63
+ L + LI I + +FL + +NLPPSP LPIIG L LLK P+HRTL S
Sbjct: 8 VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67
Query: 64 --HGPILFFWFGFRPVLVVSSPS-AAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAP 123
G ++ G R V VVSS AAEEC KND+V ANRP +I GK++GYN T+++ AP
Sbjct: 68 NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127
Query: 124 YGEHWRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLI---RRLYNTKNDVVDMRTEFFEL 183
YG+HWRNLRR+ ++EIFSTHRL +R DEVR LI RL TK VV+++ +L
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 187
Query: 184 MFNIMMRMIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGL 243
FN +MRM+ GKRYYGE+ + EEA+R R++ + +S N D++ I++ L
Sbjct: 188 TFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILR---LFSSY 247
Query: 244 ENRMKELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLV 303
ENR+K+L + D ++Q LI++ R Q ++ TMI+ LL LQ+ + YY D+ I+G++L+
Sbjct: 248 ENRVKKLGEETDKFLQGLIDDKRGQ--QETGTTMIDHLLVLQKSDIEYYTDQIIKGIILI 307
Query: 304 LLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLI 363
+++AGT S T+EW LS LLN+P+ + +A+ EID+ VG +R++EE+DL+ LPYL++++
Sbjct: 308 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVG-LDRLIEEADLSELPYLKNIV 367
Query: 364 HETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRF 423
ETLR++P PLL+PH +SEDC +G + +P GTTL VN WAI DP W +P+ F P RF
Sbjct: 368 LETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF 427
Query: 424 GSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGL 483
E E K + FG GRR CPG GL R+VGLA+GSLIQCFEWE VG +DM+EGVG
Sbjct: 428 EKEEEAQ--KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 487
Query: 484 TLPKAVPLHALCRPR 489
T+PKA+PL A+C+ R
Sbjct: 488 TVPKAIPLKAICKAR 494
BLAST of Carg21795 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 443.4 bits (1139), Expect = 2.5e-124
Identity = 238/499 (47.70%), Postives = 325/499 (65.13%), Query Frame = 0
Query: 3 IIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRHG- 62
+IF L I L L + + R NLPPSP ++LP+IG L LLK PLHR +S G
Sbjct: 6 LIFTFLFISLSLTFIIGR-IKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGD 65
Query: 63 -PILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 122
PI+ G R V VVSS S AEEC +KND+V ANR + + K++ Y T+++ A YG+H
Sbjct: 66 APIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDH 125
Query: 123 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKN---DVVDMRTEFFELMFNI 182
WRNLRRI ++EIFS HRL + SSIR DE+ LI L + V+M++ F L FN
Sbjct: 126 WRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNN 185
Query: 183 MMRMIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRM 242
++RM+AGK YYG+ A + EA+R RE+ E N D+L I+ W G E R+
Sbjct: 186 IIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRI 245
Query: 243 KELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLA 302
K++ S+ D ++Q L++E R++ + + TM++ LL LQ+ +P YY D I+G+ML L+LA
Sbjct: 246 KKIASRLDEFLQGLVDE-RREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILA 305
Query: 303 GTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETL 362
GT+ S T+EW LS LLN+P+ L +A+ EID+ VG NR++EESDL+HLPYL++++ E+L
Sbjct: 306 GTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVG-LNRLVEESDLSHLPYLQNIVSESL 365
Query: 363 RMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEG 422
R+YP PLL+PH +SEDC VGG+ +P GT L N WAI DP +W +P F P RF EG
Sbjct: 366 RLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEG 425
Query: 423 EGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE-GVGLTLP 482
E K + FG GRR CPG GL R+ L +GSLIQCFEWE +G E +DM E G G+ +P
Sbjct: 426 EAQ--KLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMP 485
Query: 483 KAVPLHALCRPRSKVAHLL 496
KA+PL A+C+ R V +L
Sbjct: 486 KAIPLVAMCKARPVVGKIL 496
BLAST of Carg21795 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 442.6 bits (1137), Expect = 4.2e-124
Identity = 235/497 (47.28%), Postives = 331/497 (66.60%), Query Frame = 0
Query: 3 IIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISR--RH 62
+IF L ++L L I+ L R NLPPSP +SLP+IG L LLK P+HRT +S+ +
Sbjct: 6 LIFSILFVVLSL-IYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 65
Query: 63 GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 122
PI G R V V SS S AEEC +KND+V ANRP+ I K++ Y+YT+++ A YG+H
Sbjct: 66 APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 125
Query: 123 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRL---YNTKNDVVDMRTEFFELMFNI 182
WRNLRRI S+EIFS HRL + SIR DE+R L+ RL ++ + VDM++ +L FN
Sbjct: 126 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNN 185
Query: 183 MMRMIAGKRYYGEDAAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRM 242
++RM+AGKRYYG+ + EA+R R++ + + N D+L +++ L E R+
Sbjct: 186 ILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLR---LVSDYETRV 245
Query: 243 KELQSKRDAWMQSLIEEHRKQSNKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLMLVLLLA 302
K+L + D ++Q L++E K+ K+ TMI+ LL+LQ+ +P Y+ D I+G ML L+LA
Sbjct: 246 KKLAGRLDEFLQGLVDE--KREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILA 305
Query: 303 GTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSLIHETL 362
GT+ S T+EW LS +LN+P+ L +A+ EID +G +R+++ESD+++LPYL++++ ETL
Sbjct: 306 GTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIG-LDRLMDESDISNLPYLQNIVSETL 365
Query: 363 RMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFNPGRFGSEG 422
R+YP P+L+PH +SEDC V G+ +P GT L NVWAI DP +W +P F P RF EG
Sbjct: 366 RLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEG 425
Query: 423 EGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVGLTLPK 482
E K MPFG GRR CPG GL R++ L +GSLIQC EWE +G E +DM EG G+T+PK
Sbjct: 426 EAQ--KLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPK 485
Query: 483 AVPLHALCRPRSKVAHL 495
A PL A+CR R V +
Sbjct: 486 AKPLEAMCRARPSVVKI 492
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 1.5e-134 | 51.45 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q6WNQ8 | 2.2e-125 | 47.57 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
P93147 | 1.4e-124 | 45.11 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q9FG65 | 5.4e-124 | 48.48 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
Q6WNQ9 | 5.1e-122 | 46.95 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FED9 | 5.0e-290 | 99.60 | isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=... | [more] |
A0A6J1INS3 | 2.0e-286 | 98.19 | isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 ... | [more] |
A0A0A0L0F1 | 2.6e-246 | 84.68 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1 | [more] |
A0A1S3BVF3 | 1.5e-241 | 82.75 | cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1 | [more] |
A0A7J0EHR3 | 5.5e-180 | 64.41 | Cytochrome P450 superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_04g000763... | [more] |
Match Name | E-value | Identity | Description | |
AT4G37360.1 | 3.5e-126 | 47.90 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37320.1 | 3.5e-126 | 47.98 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |
AT5G36220.1 | 3.8e-125 | 48.48 | cytochrome p450 81d1 | [more] |
AT4G37340.1 | 2.5e-124 | 47.70 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
AT4G37370.1 | 4.2e-124 | 47.28 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |