Carg21646 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21646
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionglutamyl-tRNA(Gln) amidotransferase subunit A
LocationCarg_Chr06: 3735106 .. 3738953 (-)
RNA-Seq ExpressionCarg21646
SyntenyCarg21646
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGGCGACTCGTACTACAGTTGCATCCAACTGCTTCTTCTTCGTCTTCTTCGTCTTCTTCTTCTTCGCAGTTCCACTCCGATCTTCCATTTCCCCCAATTTCTCTTCCTCCGATCAATCCAGACCTTGTTTCACTGCAATGGTAAGCTCGTCTTCTCTTTCTTGTTCTTTGTTCTTTTTTGTTGCGATTGTCTAATAAATCAAACCCGATCCGCCCTGCTGTATTGTGCCCTGTAGCTGTTAAAAGCAATCGACAATATTGTAAGTTTATGAATGCGTTAATTTTTTACCTTAAAAGCAGAATTATCGAGTAGAATAGTCTTGAAAATTGAGGACGCTGTTCTTTTTTTGTTGTTAAATTGCGAACTTAGTTTCCATATTGGATTTGGATATCCAGAAGTTTTTACAAAATTAGTTGAAAGGTATTATAGTCAACTTTAAGACCAAATTTGTACATTTTTCTTATTAATTTTTTTTGAAGTATTAGTTCTCTGATAAACGTAAAACAGGGAGCAACTTATAAAAAAATCTGGAAACTTTTGTGTGAAAAAGTTGGGGTTCATTACCGTTCTCCTTCCTACAGGTAACACTTGTTGGCTACTCTGCTAATGGATTGTTCTACTCAATTTTTGTCTCGTTTCTTTTCATATTCTTTTCATCAGGACAGAAACAAAGACACTGAAAGTTTCAAAAATGGTGGAAAAAGATCTTTTTTTAGAGCATTTGATGCAGACTTCTTCAGTAACACTGAGGTATACATATGTATAGCGAAATTCTTTTTTGTTTTCATGTTTTTATGTCCTGTTCTGTCCTTTTAATCCATGTAGGGCTTGGAACTTCTGTTTTTGCGTAGATGGATGAGGTTTTAAAGGGGGCTGAAGAATTTAATTTGCCTGTAATTCGAGCCAATCGGAAGCTAGTTGCTTCTGAAAATGGAGGTCTGCATAATCCTTCTCCTTTAGTGTTCAGTTCCAACTGGGCGAATGAAAGATTTCTGCATAAGAGTAGAAGATTTTGCTATCCTCCTGTTTCTGGAGTAAAGAGACCAAAAAATGAAGAAGATATTGCCTTCATGAGCGTATGTTTGTGCAGACTAACCTTTTTAGTTTTAAATGGGTTAAAATACAAGTTATAGTTTATGAACTTTCATATCTACTAGGTTCTGAACTTATAATTTTGTCTCTAAAAGATAAAGAGTAAATTGAAAGGAGCTAAATATTAGGGACTAGATTGAATAGTGATGACTTTTGTGGTTCTTGATGTGTTGGTGTCTATTAGGTTCTTGAACTTGGAGAACTGATCAAAACGAAGCAGATTACATCCCAGGAGCTCGTTGGAATATTTCTGAAGAGATTGAAAAGGTATTTATGCGCAAAATCAACTTATAGTTTTCGCTATCAAGGTTGAAATTCTTATGCAACAAATTTTTGCAAACAATACCAGGTATGACTATGTTCTTCAAGCAGTAGTTTCCTTTACTGAAGATTTGGCTTATAAACAAGCCAAAGAGGCTGATGAATTGTTCGCTCGAGGAGTGTATTTAGGTTCGTGCTTTTTAGGATTAAAATTTGTTCTGCCACTTCAATGGAAGTGGTTGATTATGGTGGTCCATCGATCAGTTGTAGTTTTCTTGAACTTTCCTCTTCATTTTTCAAATGCTGTGTTAAATTTATACATGGAGTTAATTCTTCGTTTTACAGGTCCACTACATGGAATTCCATATGGGTTGAAGGATATAATTTCTGTTCCAGGATATAAAACAACTTGGGGTTCAAAAAGTTTTAAAGAACAAGTAATTGATGTTGAAGCTTGGGTTTACCAGCAGTAAGAAAGATTTGGCTGTAAATTTCTCTCAGAAATATGTCAAGTGACAACTTTCACCAACAAATCATGAATTATTTGGTTATACAGGTTGAAGTCAGCAGGTGCTGTTCTTGTCGCTAAGCTTGTTACGGGATCGCTTGCATATGATGACATCTGGTTTGGTGGGAGAACAAGGAACCCGTGGAATATCGAGGAGTTCACAACAGGGTCATCAGCTGGACCTGCAGCATGTATTTCTGCAGGTGCCTTTTAGACTTGATTGGATTATTTCACCTTAAAATGTTGGGAAAACATCTTCCTTGTTATAAAATATTTTTGTTTGAACTCAAAAGCGAAACTGCAGCAGTTTCAGTTCGAACGGAGACTTCATGGTCTTAACAATAACCATGATTTGCTTCGGGACTACCAAGTTAGGTCTATGTGTTCCTTAAGAAAAAAAGAGCTTGTGTCGTAGTTTCTTGGAAATGACCGAGAGAGATTTGGCTTTACATCTGATGGCCCCTTCTTTTACTAGGTCTGGTTCCATTTGCTATTGGATCAGAGACAGCTGGTTCGATGACTTATCCTGCATCTCGTTGTGGAGTGACTGCACTGCGGCCAACATTTGGGACGGTTGGTCGCAGTGGAGTGATGAGTATATCCGAAAGCTTGGTAGTAGCTACGAGTCCTTTTTTCTTTTAGTTTCTCGACTCTTCTTCGGTATTCATTCTTCCTTTGCTTAACAGGACAAGCTTGGTCCATTTTGTAGAAATGCTATTGATTGTGCAGTCATTTTGGATGTCATTAGAGGAAAGGATCCACATGACCTCTCATCAGTTGAGAGTTTTCTCGATGATCCATTCTCCATCGACATTTCGAAACTTACTGTTGGATATCTCGATGATGCTGATATGGAGGTTAGTTAGTAACTCCATTAGACCAACGTCATCTGTCTTGACATCTGGATTGTTAACACAATTTGTACAATTAATGATGATTATAGGTCGTTCGGGTTCTTTCATCAAAGGGCGCAAATATGGTTCCATTCAACTTAACTTATTCCCTCGACTCTGTTCAAGGCATCCTTAACTTCACTATGGATATTGATATGTTGACTCATTTTGACGAGTGGCAACGATCGGGGCTTGATCATGAATATGAAGCTCAAGACCAATGGCCAACAGAACTGCGACGAGCACGTTTAATTCCCGCAGTAGATTATGTGCAGGTACTTTTGTTTCTTGTTGACAAATTATAGAAACGTGCGATTTCAAGTTATTTGATCATATGCACAGGCACAGAGAGCAAGGGGAAAGCTAATAAGGGAAGTTAAAGAAAGTTTCAATGTGGATGCTTTGATTGGCAATGCCACCGACTGGGAGAGGGTCTGTATGGGAAATCTTGTTGGTCTGCCTGTCATGGTTGTTCCAACAGGTTTCAAAATCATCTCAAACCCACCTGCCACGGGCACCACAAGAAGAAGAACCGCCATTACTACTGGAATCTATGCTCCTCCTCACAAGGATCACATTGTATGAACACTTTTCAACATTTTCAAGTTTTTGGAACAAAAATTTGATTCCTCCTTTAGCTTTGGATTTTGTTGAGAAGTGTGATTGGATTGATGAGATGTGTTGTTGTAGGCGTTGGCTTTGGCCATGGCGTACCAGTCTGCAACAGATCACCACAGAGCAAGGCCACCCATCGATGACTTGGGACCTCATGATGCTATTCCAGAACCACCTTTGGTTCCGATTCCTCCAAGATTGTTGCACCTTTGAGCGTGAATCTGCACTTTAATCCATGGTCTTTAATAGTCAACTCAACACCGCCATTGAAATGGTAGATCATATGACTCGTCCCAATCTGCACCTTATGTCTTTACATTTTGGAGACTTCCTTTTTCTTTTTGTATGTTCATATGACACGGAATGTTTTAGTCCTCAATTTTCTCATATGTTTCAAAGTGACTCTTTCATGTTTTTTTTAATTTATTTTAGGTCATGGTCTAGTGATTCAAACATAAATCATAAGTTCTAGTGA

mRNA sequence

ATGCCGGCGACTCGTACTACAGTTGCATCCAACTGCTTCTTCTTCGTCTTCTTCGTCTTCTTCTTCTTCGCAGTTCCACTCCGATCTTCCATTTCCCCCAATTTCTCTTCCTCCGATCAATCCAGACCTTGTTTCACTGCAATGGGAGCAACTTATAAAAAAATCTGGAAACTTTTGTGTGAAAAAGTTGGGGTTCATTACCGTTCTCCTTCCTACAGGACAGAAACAAAGACACTGAAAGTTTCAAAAATGGTGGAAAAAGATCTTTTTTTAGAGCATTTGATGCAGACTTCTTCAGTAACACTGAGGTATACATATATGGATGAGGTTTTAAAGGGGGCTGAAGAATTTAATTTGCCTGTAATTCGAGCCAATCGGAAGCTAGTTGCTTCTGAAAATGGAGGTCTGCATAATCCTTCTCCTTTAGTGTTCAGTTCCAACTGGGCGAATGAAAGATTTCTGCATAAGAGTAGAAGATTTTGCTATCCTCCTGTTTCTGGAGTAAAGAGACCAAAAAATGAAGAAGATATTGCCTTCATGAGCGTTCTTGAACTTGGAGAACTGATCAAAACGAAGCAGATTACATCCCAGGAGCTCGTTGGAATATTTCTGAAGAGATTGAAAAGGTATGACTATGTTCTTCAAGCAGTAGTTTCCTTTACTGAAGATTTGGCTTATAAACAAGCCAAAGAGGCTGATGAATTGTTCGCTCGAGGAGTGTATTTAGGTCCACTACATGGAATTCCATATGGGTTGAAGGATATAATTTCTGTTCCAGGATATAAAACAACTTGGGGTTCAAAAAGTTTTAAAGAACAAGTAATTGATGTTGAAGCTTGGGTTTACCAGCAGTTGAAGTCAGCAGGTGCTGTTCTTGTCGCTAAGCTTGTTACGGGATCGCTTGCATATGATGACATCTGGTTTGGTGGGAGAACAAGGAACCCGTGGAATATCGAGGAGTTCACAACAGGGTCATCAGCTGGACCTGCAGCATGTATTTCTGCAGGTCTGGTTCCATTTGCTATTGGATCAGAGACAGCTGGTTCGATGACTTATCCTGCATCTCGTTGTGGAGTGACTGCACTGCGGCCAACATTTGGGACGGTTGGTCGCAGTGGAGTGATGAGTATATCCGAAAGCTTGGACAAGCTTGGTCCATTTTGTAGAAATGCTATTGATTGTGCAGTCATTTTGGATGTCATTAGAGGAAAGGATCCACATGACCTCTCATCAGTTGAGAGTTTTCTCGATGATCCATTCTCCATCGACATTTCGAAACTTACTGTTGGATATCTCGATGATGCTGATATGGAGGTCGTTCGGGTTCTTTCATCAAAGGGCGCAAATATGGTTCCATTCAACTTAACTTATTCCCTCGACTCTGTTCAAGGCATCCTTAACTTCACTATGGATATTGATATGTTGACTCATTTTGACGAGTGGCAACGATCGGGGCTTGATCATGAATATGAAGCTCAAGACCAATGGCCAACAGAACTGCGACGAGCACGTTTAATTCCCGCAGTAGATTATGTGCAGGCACAGAGAGCAAGGGGAAAGCTAATAAGGGAAGTTAAAGAAAGTTTCAATGTGGATGCTTTGATTGGCAATGCCACCGACTGGGAGAGGGTCTGTATGGGAAATCTTGTTGGTCTGCCTGTCATGGTTGTTCCAACAGGTTTCAAAATCATCTCAAACCCACCTGCCACGGGCACCACAAGAAGAAGAACCGCCATTACTACTGGAATCTATGCTCCTCCTCACAAGGATCACATTGCGTTGGCTTTGGCCATGGCGTACCAGTCTGCAACAGATCACCACAGAGCAAGGCCACCCATCGATGACTTGGGACCTCATGATGCTATTCCAGAACCACCTTTGGTTCCGATTCCTCCAAGATTGTTGCACCTTTGAGCGTGAATCTGCACTTTAATCCATGGTCTTTAATAGTCAACTCAACACCGCCATTGAAATGGTAGATCATATGACTCGTCCCAATCTGCACCTTATGTCTTTACATTTTGGAGACTTCCTTTTTCTTTTTGTATGTTCATATGACACGGAATGTTTTAGTCCTCAATTTTCTCATATGTTTCAAAGTGACTCTTTCATGTTTTTTTTAATTTATTTTAGGTCATGGTCTAGTGATTCAAACATAAATCATAAGTTCTAGTGA

Coding sequence (CDS)

ATGCCGGCGACTCGTACTACAGTTGCATCCAACTGCTTCTTCTTCGTCTTCTTCGTCTTCTTCTTCTTCGCAGTTCCACTCCGATCTTCCATTTCCCCCAATTTCTCTTCCTCCGATCAATCCAGACCTTGTTTCACTGCAATGGGAGCAACTTATAAAAAAATCTGGAAACTTTTGTGTGAAAAAGTTGGGGTTCATTACCGTTCTCCTTCCTACAGGACAGAAACAAAGACACTGAAAGTTTCAAAAATGGTGGAAAAAGATCTTTTTTTAGAGCATTTGATGCAGACTTCTTCAGTAACACTGAGGTATACATATATGGATGAGGTTTTAAAGGGGGCTGAAGAATTTAATTTGCCTGTAATTCGAGCCAATCGGAAGCTAGTTGCTTCTGAAAATGGAGGTCTGCATAATCCTTCTCCTTTAGTGTTCAGTTCCAACTGGGCGAATGAAAGATTTCTGCATAAGAGTAGAAGATTTTGCTATCCTCCTGTTTCTGGAGTAAAGAGACCAAAAAATGAAGAAGATATTGCCTTCATGAGCGTTCTTGAACTTGGAGAACTGATCAAAACGAAGCAGATTACATCCCAGGAGCTCGTTGGAATATTTCTGAAGAGATTGAAAAGGTATGACTATGTTCTTCAAGCAGTAGTTTCCTTTACTGAAGATTTGGCTTATAAACAAGCCAAAGAGGCTGATGAATTGTTCGCTCGAGGAGTGTATTTAGGTCCACTACATGGAATTCCATATGGGTTGAAGGATATAATTTCTGTTCCAGGATATAAAACAACTTGGGGTTCAAAAAGTTTTAAAGAACAAGTAATTGATGTTGAAGCTTGGGTTTACCAGCAGTTGAAGTCAGCAGGTGCTGTTCTTGTCGCTAAGCTTGTTACGGGATCGCTTGCATATGATGACATCTGGTTTGGTGGGAGAACAAGGAACCCGTGGAATATCGAGGAGTTCACAACAGGGTCATCAGCTGGACCTGCAGCATGTATTTCTGCAGGTCTGGTTCCATTTGCTATTGGATCAGAGACAGCTGGTTCGATGACTTATCCTGCATCTCGTTGTGGAGTGACTGCACTGCGGCCAACATTTGGGACGGTTGGTCGCAGTGGAGTGATGAGTATATCCGAAAGCTTGGACAAGCTTGGTCCATTTTGTAGAAATGCTATTGATTGTGCAGTCATTTTGGATGTCATTAGAGGAAAGGATCCACATGACCTCTCATCAGTTGAGAGTTTTCTCGATGATCCATTCTCCATCGACATTTCGAAACTTACTGTTGGATATCTCGATGATGCTGATATGGAGGTCGTTCGGGTTCTTTCATCAAAGGGCGCAAATATGGTTCCATTCAACTTAACTTATTCCCTCGACTCTGTTCAAGGCATCCTTAACTTCACTATGGATATTGATATGTTGACTCATTTTGACGAGTGGCAACGATCGGGGCTTGATCATGAATATGAAGCTCAAGACCAATGGCCAACAGAACTGCGACGAGCACGTTTAATTCCCGCAGTAGATTATGTGCAGGCACAGAGAGCAAGGGGAAAGCTAATAAGGGAAGTTAAAGAAAGTTTCAATGTGGATGCTTTGATTGGCAATGCCACCGACTGGGAGAGGGTCTGTATGGGAAATCTTGTTGGTCTGCCTGTCATGGTTGTTCCAACAGGTTTCAAAATCATCTCAAACCCACCTGCCACGGGCACCACAAGAAGAAGAACCGCCATTACTACTGGAATCTATGCTCCTCCTCACAAGGATCACATTGCGTTGGCTTTGGCCATGGCGTACCAGTCTGCAACAGATCACCACAGAGCAAGGCCACCCATCGATGACTTGGGACCTCATGATGCTATTCCAGAACCACCTTTGGTTCCGATTCCTCCAAGATTGTTGCACCTTTGA

Protein sequence

MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLCEKVGVHYRSPSYRTETKTLKVSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDEWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATDWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL
Homology
BLAST of Carg21646 vs. NCBI nr
Match: KAG7028327.1 (gatA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1278.8 bits (3308), Expect = 0.0e+00
Identity = 637/637 (100.00%), Postives = 637/637 (100.00%), Query Frame = 0

Query: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60
           MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC
Sbjct: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60

Query: 61  EKVGVHYRSPSYRTETKTLKVSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNLP 120
           EKVGVHYRSPSYRTETKTLKVSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNLP
Sbjct: 61  EKVGVHYRSPSYRTETKTLKVSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNLP 120

Query: 121 VIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFM 180
           VIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFM
Sbjct: 121 VIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFM 180

Query: 181 SVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV 240
           SVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV
Sbjct: 181 SVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV 240

Query: 241 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS 300
           YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS
Sbjct: 241 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS 300

Query: 301 LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVT 360
           LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVT
Sbjct: 301 LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVT 360

Query: 361 ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPF 420
           ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPF
Sbjct: 361 ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPF 420

Query: 421 SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDE 480
           SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDE
Sbjct: 421 SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDE 480

Query: 481 WQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATD 540
           WQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATD
Sbjct: 481 WQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATD 540

Query: 541 WERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY 600
           WERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY
Sbjct: 541 WERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY 600

Query: 601 QSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           QSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL
Sbjct: 601 QSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 637

BLAST of Carg21646 vs. NCBI nr
Match: XP_022946706.1 (uncharacterized protein LOC111450695 [Cucurbita moschata])

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 602/644 (93.48%), Postives = 609/644 (94.57%), Query Frame = 0

Query: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60
           MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC
Sbjct: 9   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 68

Query: 61  EKVGVHYRSPSYRT-ETKTLK------VSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKG 120
           EKVGVHYRSPSYR  +T++ K        +  + D F              T MDEVLKG
Sbjct: 69  EKVGVHYRSPSYRNKDTESFKNGGKRSFFRAFDADFFSN------------TEMDEVLKG 128

Query: 121 AEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKN 180
           AEE NLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKN
Sbjct: 129 AEELNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKN 188

Query: 181 EEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEAD 240
           EEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEAD
Sbjct: 189 EEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEAD 248

Query: 241 ELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLV 300
           ELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLV
Sbjct: 249 ELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLV 308

Query: 301 AKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYP 360
            KLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAAC SAGLVPFAIGSETAGSMTYP
Sbjct: 309 TKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTYP 368

Query: 361 ASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVE 420
           ASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVE
Sbjct: 369 ASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVE 428

Query: 421 SFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDID 480
           SFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDID
Sbjct: 429 SFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDID 488

Query: 481 MLTHFDEWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDA 540
           MLTHFDEWQRSGLDHEYEA+DQWPTELRRARLIPAVDYVQAQRARGKL REVKESFNVDA
Sbjct: 489 MLTHFDEWQRSGLDHEYEAEDQWPTELRRARLIPAVDYVQAQRARGKLRREVKESFNVDA 548

Query: 541 LIGNATDWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIA 600
           LIGNATDWERVCMGNLVGLPV+VVPTGFK ISNPPATGTTRRRTAITTGIYAPPHKDHIA
Sbjct: 549 LIGNATDWERVCMGNLVGLPVIVVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHIA 608

Query: 601 LALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           LALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL
Sbjct: 609 LALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 640

BLAST of Carg21646 vs. NCBI nr
Match: XP_023540352.1 (uncharacterized protein LOC111800751 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 599/645 (92.87%), Postives = 607/645 (94.11%), Query Frame = 0

Query: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60
           MPATRTTVASNCFFFVFFVF FFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC
Sbjct: 9   MPATRTTVASNCFFFVFFVFIFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 68

Query: 61  EKVGVHYRS-PSYRT-ETKTLK------VSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLK 120
           EKVGVHYRS PSYR  +T++ K        +  + D F              T MDEVLK
Sbjct: 69  EKVGVHYRSPPSYRNKDTESFKNGGKRSFFRAFDADFFSN------------TEMDEVLK 128

Query: 121 GAEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPK 180
           GAEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERF HKSRRFCYPPVSGVKRPK
Sbjct: 129 GAEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFQHKSRRFCYPPVSGVKRPK 188

Query: 181 NEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEA 240
           NEEDIAFM+VLELGELIKTKQITSQELVGIFL+RLKRYDYVLQAVVSFTEDLAYKQAKEA
Sbjct: 189 NEEDIAFMNVLELGELIKTKQITSQELVGIFLQRLKRYDYVLQAVVSFTEDLAYKQAKEA 248

Query: 241 DELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVL 300
           DELFA+GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVL
Sbjct: 249 DELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVL 308

Query: 301 VAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTY 360
           VAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAAC SAGLVPFAIGSETAGSMTY
Sbjct: 309 VAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTY 368

Query: 361 PASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSV 420
           PASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSV
Sbjct: 369 PASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSV 428

Query: 421 ESFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDI 480
           ES LDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDI
Sbjct: 429 ESSLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDI 488

Query: 481 DMLTHFDEWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVD 540
           DML HFDEWQRS LDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVD
Sbjct: 489 DMLAHFDEWQRSRLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVD 548

Query: 541 ALIGNATDWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHI 600
           ALIGNATDWERVCMGNLVGLPVMVVPTGFK ISNPPATGTTRRRTAITTGIYAPPHKDHI
Sbjct: 549 ALIGNATDWERVCMGNLVGLPVMVVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHI 608

Query: 601 ALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           ALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL
Sbjct: 609 ALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 641

BLAST of Carg21646 vs. NCBI nr
Match: XP_023005329.1 (uncharacterized protein LOC111498360 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 592/638 (92.79%), Postives = 598/638 (93.73%), Query Frame = 0

Query: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60
           MPATRT +ASNCFFFVFFVF FFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC
Sbjct: 9   MPATRTAIASNCFFFVFFVFIFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 68

Query: 61  EKVGVHYRS-PSYRTETKTLKVSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNL 120
           EKVG HYRS PSYR   K  +  K   K  F        +     T MDEVLKGAEEFN 
Sbjct: 69  EKVGDHYRSPPSYR--NKDTESFKNGGKRSFFRAF---DAEFFSKTEMDEVLKGAEEFNF 128

Query: 121 PVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAF 180
           PVIRANRKLVASENGGLHNPSPLVFSSNWANE F HKSRRFCYPPV GVKRPKNEEDIAF
Sbjct: 129 PVIRANRKLVASENGGLHNPSPLVFSSNWANEGFQHKSRRFCYPPVFGVKRPKNEEDIAF 188

Query: 181 MSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARG 240
           MSVLELGELIKTKQITSQELVGIFL+RLKRYDYVLQAVVSFTEDLAYKQAKEADELFARG
Sbjct: 189 MSVLELGELIKTKQITSQELVGIFLQRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARG 248

Query: 241 VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTG 300
           VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTG
Sbjct: 249 VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTG 308

Query: 301 SLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGV 360
           SLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAAC SAGLVPFAIGSETAGSMTYPASRCGV
Sbjct: 309 SLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTYPASRCGV 368

Query: 361 TALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDP 420
           TALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVES LDDP
Sbjct: 369 TALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESSLDDP 428

Query: 421 FSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFD 480
           FSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDML HFD
Sbjct: 429 FSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLAHFD 488

Query: 481 EWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNAT 540
           EWQRSGLDHEYEAQDQWPTELRRARLIPAVDY+QAQRARGKLIREVKESFNVDALIGNAT
Sbjct: 489 EWQRSGLDHEYEAQDQWPTELRRARLIPAVDYLQAQRARGKLIREVKESFNVDALIGNAT 548

Query: 541 DWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMA 600
           DWERVCMGNLVGLPVMVVPTGFK ISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMA
Sbjct: 549 DWERVCMGNLVGLPVMVVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMA 608

Query: 601 YQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           YQSATDHHRARPPIDDLGPHDAIPEPP VPIPPRLL +
Sbjct: 609 YQSATDHHRARPPIDDLGPHDAIPEPPSVPIPPRLLRV 641

BLAST of Carg21646 vs. NCBI nr
Match: KAG6596801.1 (hypothetical protein SDJN03_09981, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 584/637 (91.68%), Postives = 590/637 (92.62%), Query Frame = 0

Query: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60
           MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAM            
Sbjct: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMDR---------- 60

Query: 61  EKVGVHYRSPSYRTETKTLKVSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNLP 120
                +  + S++   K     +  + D F              T MDEVLKGAEEFNLP
Sbjct: 61  -----NKDTESFKNGGKR-SFFRAFDADFFSN------------TEMDEVLKGAEEFNLP 120

Query: 121 VIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFM 180
           VIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFM
Sbjct: 121 VIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFM 180

Query: 181 SVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV 240
           SVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV
Sbjct: 181 SVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV 240

Query: 241 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS 300
           YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS
Sbjct: 241 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS 300

Query: 301 LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVT 360
           LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVT
Sbjct: 301 LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVT 360

Query: 361 ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPF 420
           ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPF
Sbjct: 361 ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPF 420

Query: 421 SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDE 480
           SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDE
Sbjct: 421 SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDE 480

Query: 481 WQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATD 540
           WQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATD
Sbjct: 481 WQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATD 540

Query: 541 WERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY 600
           WERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY
Sbjct: 541 WERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY 600

Query: 601 QSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           QSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL
Sbjct: 601 QSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 609

BLAST of Carg21646 vs. ExPASy Swiss-Prot
Match: Q3AD36 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=gatA PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 8.9e-47
Identity = 149/498 (29.92%), Postives = 229/498 (45.98%), Query Frame = 0

Query: 176 DIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADEL 235
           ++ ++S  E+ E IK K+I++ E+      R++  +  +QA V+ T +L  K A+E DE 
Sbjct: 2   ELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDEK 61

Query: 236 FARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAK 295
            ARG   GPL G+P  +KD +S  G +TT  SK  +  +   +A V ++LK AGAV   K
Sbjct: 62  IARGEDPGPLAGVPVAIKDNMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTGK 121

Query: 296 LVTGSLAYDDIWFGGR---TRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTY 355
                 A        R   TRNPW++E    GSS G AA ++AG    A+GS+T GS+  
Sbjct: 122 TNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAASVAAGEAVVALGSDTGGSIRQ 181

Query: 356 PASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSV 415
           PA+ CG+  L+PT+G V R G+++ + SLD++GPF R   D A++L+VI G DP D +S 
Sbjct: 182 PAAFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPFARTVEDAALLLNVIAGHDPKDSTSA 241

Query: 416 ESFLDDPFSI---DISKLTVG-----YLDDADM-------EVVRVLSSKGANMVPFNL-- 475
           +    D  S    DI  L +G     ++D  D        + ++VL S GA     +L  
Sbjct: 242 DIEYPDYLSFLNQDIKGLKIGLPKEYFIDGIDAGVKKAIDDAIKVLESLGAVFEEVSLPH 301

Query: 476 -TYSL------------------DSVQ-GILNFTMDIDMLTHFDEWQRSGLDHEYEAQDQ 535
             YSL                  D V+ G  +F  + D++  F   +  G   E + +  
Sbjct: 302 TKYSLPVYYLIAPAEASSNLARYDGVRYGYRDFEAE-DVVEMFSRTRAEGFGAEVKRRIM 361

Query: 536 WPTELRRARLIPAVDYVQAQRARGKLIREVKESF-NVDALIGNATDWERVCMG------- 595
             T    A    A  Y++A + R  +  +   +F  VD L+   T       G       
Sbjct: 362 LGTYALSAGYYDAY-YLKALKVRTLIKEDFDRAFTKVDLLLTPTTPTPAFKFGEKTSDPV 421

Query: 596 ------------NLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALA 614
                       NL GLP + VP GF                 ++  +   P  +   L 
Sbjct: 422 SMYLSDIFTMAVNLAGLPGISVPAGFD------------GHLPVSFQLIGKPFDEGTLLK 481

BLAST of Carg21646 vs. ExPASy Swiss-Prot
Match: B2V855 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Sulfurihydrogenibium sp. (strain YO3AOP1) OX=436114 GN=gatA PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.7e-43
Identity = 139/494 (28.14%), Postives = 239/494 (48.38%), Query Frame = 0

Query: 181 SVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV 240
           S+ EL +L+K+K++   E+V  F++R  + +  ++A V+  +DLA ++AK+ D+   +  
Sbjct: 7   SLKELSDLVKSKEVKPSEIVEAFIERKNQVEPKIKAYVTALDDLALEKAKKRDQELTKLE 66

Query: 241 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAK----- 300
            +  L G+P  +KD IS    KTT  SK  +  V   +A V ++LKS G V+  K     
Sbjct: 67  NIPDLFGLPIAIKDNISTKDIKTTCSSKMLENFVPVYDATVIERLKSQGYVITGKTNLDE 126

Query: 301 LVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPAS 360
              GS   +  +F   TRNPW++E    GSS G AA +++G+ P ++GS+T GS+  PA+
Sbjct: 127 FAMGSSTENSAFF--PTRNPWDLERVPGGSSGGSAAVVASGMAPASLGSDTGGSIRQPAA 186

Query: 361 RCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSS---- 420
            CGV  L+PT+G V R G+++ + SLD++GPF R   D A+I++VI GKDP D +S    
Sbjct: 187 FCGVVGLKPTYGRVSRYGLVAFASSLDQIGPFGRTVEDVAMIMNVISGKDPKDSTSRSIP 246

Query: 421 VESFLDDPFSIDISKLTVG-----YLDDADMEV-------VRVLSSKGANMVPFNLTYSL 480
           V ++L+   + D+  L +G     Y +D + ++       V+ L  +G      +L Y+ 
Sbjct: 247 VPNYLES-LNKDVKGLKIGLPKEFYTEDLNPQIKEIILNAVKQLEKEGMTAHEISLPYTK 306

Query: 481 DSVQG--ILNFTMDIDMLTHFDEWQRSGLDHEYEAQDQ---------WPTELRRARLIPA 540
            +++   I+  +     L  FD  +      EY+  ++         +  E++R  +I  
Sbjct: 307 YAIETYYIIAPSEASSNLARFDGVRYGYRAKEYKNLEEMYSKTRDEGFGAEVKRRIMIGT 366

Query: 541 ---------VDYVQAQRARGKLIREVKESF-NVDALIGNATDWERVCMG----------- 600
                      Y++AQ+ R  + ++   +F  VD +I   T      +G           
Sbjct: 367 YALSSGYYDAYYLKAQKVRTLIYQDYMNAFEKVDVIITPTTPDVAFKIGEKSNDPIQMYL 426

Query: 601 --------NLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMA 614
                   N+  +P + +P GFK   N P          +   I   P  +   L +A  
Sbjct: 427 SDIFTVSANMATVPALSIPCGFK--DNLP----------VGMQIIGKPFDEETILQVAYK 485

BLAST of Carg21646 vs. ExPASy Swiss-Prot
Match: Q2RGY4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Moorella thermoacetica (strain ATCC 39073 / JCM 9320) OX=264732 GN=gatA PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 3.5e-43
Identity = 93/243 (38.27%), Postives = 147/243 (60.49%), Query Frame = 0

Query: 176 DIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADEL 235
           ++ +++  EL +L+  K+I+S+E     + R++  D  +QA ++ T + A ++A+  D  
Sbjct: 2   ELYYLTAHELSDLLNRKEISSEEATAAIIDRIEAVDGRVQAYLTRTAEQALEEARAVDAA 61

Query: 236 FARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAK 295
            ARG  LGPL G+P  LKD +   G +TT  S+   + V   +A V ++LK AGAV++ K
Sbjct: 62  RARGETLGPLAGVPMALKDNLCTEGVRTTCSSRMLADWVPPYDATVVRRLKEAGAVMLGK 121

Query: 296 L-----VTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSM 355
           L       GS   +  +F   TRNPW++E    GSS G  A ++AG   FA+GS+T GS+
Sbjct: 122 LNMDEFAMGSSTENSSFF--PTRNPWDLERVPGGSSGGAVAAVAAGEAYFALGSDTGGSI 181

Query: 356 TYPASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLS 414
             PAS CGV  ++PT+G V R G+++ + SLD++GP  R+  DCA++L+ I G DP D +
Sbjct: 182 RQPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPITRDVTDCALVLEAIAGHDPLDST 241

BLAST of Carg21646 vs. ExPASy Swiss-Prot
Match: A5UXF9 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=gatA PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 8.7e-42
Identity = 149/493 (30.22%), Postives = 225/493 (45.64%), Query Frame = 0

Query: 180 MSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARG 239
           +++ +  E +   +ITS EL    L R+   +  ++A ++   + A  QA+ AD   A G
Sbjct: 7   LTLSDAREALMRGEITSVELTDALLARIAAVEPQVRAFLTIDAEGARAQAQAADARRAAG 66

Query: 240 VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTG 299
               PL GIP G+KD+IS  G +TT  SK  +  V   +A    +LK+AGAVL+ KL   
Sbjct: 67  -DTSPLLGIPLGIKDVISTQGVRTTCASKMLENYVPVYDATAVARLKAAGAVLIGKLNCD 126

Query: 300 SLAYDDIWFGG---RTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASR 359
             A           +TRNPWN+E    GSS G AA ++AG  P  +G++T GS+  PA+ 
Sbjct: 127 EFAMGSSTENSAFQQTRNPWNLERVPGGSSGGSAAAVAAGEAPATLGTDTGGSIRQPAAL 186

Query: 360 CGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFL 419
           CG+T L+PT+G V R G+++ + SLD++GP  R   DCA+IL VI G DP D +  +  +
Sbjct: 187 CGITGLKPTYGRVSRYGLVAFASSLDQIGPMARTVRDCAIILRVIAGADPFDATCTDHPV 246

Query: 420 DD---PFSIDISKLTVGY------------LDDADMEVVRVLSSKGAN------------ 479
            D     + DI  L +G             ++ A    + VL  +GA             
Sbjct: 247 PDYEAALTGDIRGLRIGVPREYFVAGMQPEVESAVRVAIDVLRDQGAEVREISLPHTPYA 306

Query: 480 ------MVPFNLTYSLDSVQGILNFTMDIDMLTHFDEWQR---SGLDHEYEAQDQWPTEL 539
                 + P   + +L    G+  + + +   ++FDE +R   +G   E   +    T  
Sbjct: 307 LPVYYLIAPAEASANLARFDGV-RYGLRVPGESYFDELERTRGAGFGPEVRRRIMLGTYA 366

Query: 540 RRARLIPAVDYVQAQRARGKLIREVKESF-NVDAL-----------IGNATD------WE 599
             A    A  Y +AQ+ R  + R+ +++F  VD +           IG  +D       E
Sbjct: 367 LSAGYYDAY-YKRAQQVRTLIRRDYQQAFEQVDVIAAPTTPTVAFPIGAHSDDPLAMYLE 426

Query: 600 RVCM--GNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY 614
            VC    NL GLP +VVP GF       A G       I   +      +   L +  AY
Sbjct: 427 DVCTLPLNLAGLPGLVVPCGF-------AAG-----LPIGLQLIGRAFDEETLLRIGDAY 484

BLAST of Carg21646 vs. ExPASy Swiss-Prot
Match: P63497 (Putative amidase AmiD OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=amiD PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.5e-41
Identity = 140/479 (29.23%), Postives = 222/479 (46.35%), Query Frame = 0

Query: 166 SGVKRPKNEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLA 225
           S V    +E+ I+ + + E+ +LI+T+Q+TS E+    L+R++R D  L++      + A
Sbjct: 6   SAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETA 65

Query: 226 YKQAKEADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQL 285
              A+ AD   ARG Y G LHG+P G+KD+       T  G+  F++     +A V  +L
Sbjct: 66  LAAARAADADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARL 125

Query: 286 KSAGAVLVAKLVTGSLAYDDIWFGGRTR-NPWNIEEFTTGSSAGPAACISAGLVPFAIGS 345
           ++AGAV++ KL     AY        T  NPW+   +   SS+G     +AGL   +IGS
Sbjct: 126 RAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGS 185

Query: 346 ETAGSMTYPASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGK 405
           +T GS+ +P S CGVT ++PT+G V R GV+ ++ S D +GP  R+A D AV+L VI G 
Sbjct: 186 DTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGS 245

Query: 406 DPHDLS-SVESFLDDPFSIDISKL---------TVGYLDDAD---MEVVRVLSSKGANMV 465
           D HD S S E   D    + ++++         T  + +D      +VV+ L   G  ++
Sbjct: 246 DIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVI 305

Query: 466 PFNLTYSLDSVQGILNFTMDIDMLTHFDEWQRSGLDHEYEAQDQWPTELRRARL-----I 525
              L      V            + H D          Y A+      + RA +     +
Sbjct: 306 DVKLPALAPMVAAFGKMRAVETAIAHAD---------TYPARADEYGPIMRAMIDAGHRL 365

Query: 526 PAVDYVQAQRARGKLIREVKESF-NVDALIGNATDWERVCMGNLVGL-------PVMVVP 585
            AV+Y      R +  R ++  F +VD L+  +       +  + GL         + +P
Sbjct: 366 AAVEYQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMP 425

Query: 586 TG-FKIISNPP---ATGTTRRRTAITTGIYAPPHKDHIALALAMAYQSATDHHRARPPI 614
           T  F +  NP      GTT R T +          +H+ +    A+Q  T +HR RPP+
Sbjct: 426 TAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475

BLAST of Carg21646 vs. ExPASy TrEMBL
Match: A0A6J1G4E4 (uncharacterized protein LOC111450695 OS=Cucurbita moschata OX=3662 GN=LOC111450695 PE=4 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 602/644 (93.48%), Postives = 609/644 (94.57%), Query Frame = 0

Query: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60
           MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC
Sbjct: 9   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 68

Query: 61  EKVGVHYRSPSYRT-ETKTLK------VSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKG 120
           EKVGVHYRSPSYR  +T++ K        +  + D F              T MDEVLKG
Sbjct: 69  EKVGVHYRSPSYRNKDTESFKNGGKRSFFRAFDADFFSN------------TEMDEVLKG 128

Query: 121 AEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKN 180
           AEE NLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKN
Sbjct: 129 AEELNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKN 188

Query: 181 EEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEAD 240
           EEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEAD
Sbjct: 189 EEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEAD 248

Query: 241 ELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLV 300
           ELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLV
Sbjct: 249 ELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLV 308

Query: 301 AKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYP 360
            KLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAAC SAGLVPFAIGSETAGSMTYP
Sbjct: 309 TKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTYP 368

Query: 361 ASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVE 420
           ASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVE
Sbjct: 369 ASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVE 428

Query: 421 SFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDID 480
           SFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDID
Sbjct: 429 SFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDID 488

Query: 481 MLTHFDEWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDA 540
           MLTHFDEWQRSGLDHEYEA+DQWPTELRRARLIPAVDYVQAQRARGKL REVKESFNVDA
Sbjct: 489 MLTHFDEWQRSGLDHEYEAEDQWPTELRRARLIPAVDYVQAQRARGKLRREVKESFNVDA 548

Query: 541 LIGNATDWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIA 600
           LIGNATDWERVCMGNLVGLPV+VVPTGFK ISNPPATGTTRRRTAITTGIYAPPHKDHIA
Sbjct: 549 LIGNATDWERVCMGNLVGLPVIVVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHIA 608

Query: 601 LALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           LALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL
Sbjct: 609 LALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 640

BLAST of Carg21646 vs. ExPASy TrEMBL
Match: A0A6J1KYV3 (uncharacterized protein LOC111498360 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498360 PE=4 SV=1)

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 592/638 (92.79%), Postives = 598/638 (93.73%), Query Frame = 0

Query: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60
           MPATRT +ASNCFFFVFFVF FFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC
Sbjct: 9   MPATRTAIASNCFFFVFFVFIFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 68

Query: 61  EKVGVHYRS-PSYRTETKTLKVSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNL 120
           EKVG HYRS PSYR   K  +  K   K  F        +     T MDEVLKGAEEFN 
Sbjct: 69  EKVGDHYRSPPSYR--NKDTESFKNGGKRSFFRAF---DAEFFSKTEMDEVLKGAEEFNF 128

Query: 121 PVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAF 180
           PVIRANRKLVASENGGLHNPSPLVFSSNWANE F HKSRRFCYPPV GVKRPKNEEDIAF
Sbjct: 129 PVIRANRKLVASENGGLHNPSPLVFSSNWANEGFQHKSRRFCYPPVFGVKRPKNEEDIAF 188

Query: 181 MSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARG 240
           MSVLELGELIKTKQITSQELVGIFL+RLKRYDYVLQAVVSFTEDLAYKQAKEADELFARG
Sbjct: 189 MSVLELGELIKTKQITSQELVGIFLQRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARG 248

Query: 241 VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTG 300
           VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTG
Sbjct: 249 VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTG 308

Query: 301 SLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGV 360
           SLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAAC SAGLVPFAIGSETAGSMTYPASRCGV
Sbjct: 309 SLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTYPASRCGV 368

Query: 361 TALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDP 420
           TALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVES LDDP
Sbjct: 369 TALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESSLDDP 428

Query: 421 FSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFD 480
           FSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDML HFD
Sbjct: 429 FSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLAHFD 488

Query: 481 EWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNAT 540
           EWQRSGLDHEYEAQDQWPTELRRARLIPAVDY+QAQRARGKLIREVKESFNVDALIGNAT
Sbjct: 489 EWQRSGLDHEYEAQDQWPTELRRARLIPAVDYLQAQRARGKLIREVKESFNVDALIGNAT 548

Query: 541 DWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMA 600
           DWERVCMGNLVGLPVMVVPTGFK ISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMA
Sbjct: 549 DWERVCMGNLVGLPVMVVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMA 608

Query: 601 YQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           YQSATDHHRARPPIDDLGPHDAIPEPP VPIPPRLL +
Sbjct: 609 YQSATDHHRARPPIDDLGPHDAIPEPPSVPIPPRLLRV 641

BLAST of Carg21646 vs. ExPASy TrEMBL
Match: A0A6J1KX47 (uncharacterized protein LOC111498360 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498360 PE=4 SV=1)

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 567/637 (89.01%), Postives = 579/637 (90.89%), Query Frame = 0

Query: 1   MPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQSRPCFTAMGATYKKIWKLLC 60
           MPATRT +ASNCFFFVFFVF FFAVPLRSSISPNFSSSDQSRPCFTAM            
Sbjct: 9   MPATRTAIASNCFFFVFFVFIFFAVPLRSSISPNFSSSDQSRPCFTAMDR---------- 68

Query: 61  EKVGVHYRSPSYRTETKTLKVSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNLP 120
                +  + S++   K     +  + + F +            T MDEVLKGAEEFN P
Sbjct: 69  -----NKDTESFKNGGKR-SFFRAFDAEFFSK------------TEMDEVLKGAEEFNFP 128

Query: 121 VIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFM 180
           VIRANRKLVASENGGLHNPSPLVFSSNWANE F HKSRRFCYPPV GVKRPKNEEDIAFM
Sbjct: 129 VIRANRKLVASENGGLHNPSPLVFSSNWANEGFQHKSRRFCYPPVFGVKRPKNEEDIAFM 188

Query: 181 SVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV 240
           SVLELGELIKTKQITSQELVGIFL+RLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV
Sbjct: 189 SVLELGELIKTKQITSQELVGIFLQRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGV 248

Query: 241 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS 300
           YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS
Sbjct: 249 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGS 308

Query: 301 LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVT 360
           LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAAC SAGLVPFAIGSETAGSMTYPASRCGVT
Sbjct: 309 LAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTYPASRCGVT 368

Query: 361 ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPF 420
           ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVES LDDPF
Sbjct: 369 ALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESSLDDPF 428

Query: 421 SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDE 480
           SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDML HFDE
Sbjct: 429 SIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLAHFDE 488

Query: 481 WQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATD 540
           WQRSGLDHEYEAQDQWPTELRRARLIPAVDY+QAQRARGKLIREVKESFNVDALIGNATD
Sbjct: 489 WQRSGLDHEYEAQDQWPTELRRARLIPAVDYLQAQRARGKLIREVKESFNVDALIGNATD 548

Query: 541 WERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY 600
           WERVCMGNLVGLPVMVVPTGFK ISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY
Sbjct: 549 WERVCMGNLVGLPVMVVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAY 608

Query: 601 QSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           QSATDHHRARPPIDDLGPHDAIPEPP VPIPPRLL +
Sbjct: 609 QSATDHHRARPPIDDLGPHDAIPEPPSVPIPPRLLRV 617

BLAST of Carg21646 vs. ExPASy TrEMBL
Match: A0A0A0L5U7 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G039330 PE=4 SV=1)

HSP 1 Score: 999.2 bits (2582), Expect = 7.8e-288
Identity = 511/638 (80.09%), Postives = 556/638 (87.15%), Query Frame = 0

Query: 2   PATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQ-SRPCFTAMGATYKKIWKLLC 61
           P   +T   NC F VFF FFFF V +RSSIS +FSS  Q S   FT M +  KK  + L 
Sbjct: 73  PTMASTPTINC-FVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMDS--KKDSECL- 132

Query: 62  EKVGVHYRSPSYRTETKTLK-VSKMVEKDLFLEHLMQTSSVTLRYTYMDEVLKGAEEFNL 121
           E +G           T  LK V + ++ D F              T  DEVLKGAEE N+
Sbjct: 133 ENMG----------RTSVLKCVFRALDADFF------------NNTKRDEVLKGAEELNV 192

Query: 122 PVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAF 181
           P+IRANRKLVASENGGLHNPS LVF+  WANE   HKS+RFCYPPVSG+KRP NE+DIAF
Sbjct: 193 PIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAF 252

Query: 182 MSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARG 241
           MSVLELGELIKT+QI+SQELV IFL+RLKRY++VL+AVVSFTE+LAYKQAKEADELFA+G
Sbjct: 253 MSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQG 312

Query: 242 VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTG 301
           VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVY+QLKSAGAVLVAKLVTG
Sbjct: 313 VYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTG 372

Query: 302 SLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGV 361
           SLAYDDIWFGGRTRNPWNIEEF+TGSSAGPAAC SAG+VPFAIGSET GSMTYPA+RCGV
Sbjct: 373 SLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGV 432

Query: 362 TALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDP 421
           TA+RPTFGTVGRSGVMSISESLDKLGPFCRNA+DCAV+LDVIRGKDPHDLSSVES LDDP
Sbjct: 433 TAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDP 492

Query: 422 FSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFD 481
           FSIDISKLTVGYLDDADMEVVR+LSSKG NMVPFNL+YS+DSVQGI+NFTMD+DML HFD
Sbjct: 493 FSIDISKLTVGYLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFD 552

Query: 482 EWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNAT 541
           EWQR GLD EYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREV+ESFNVDALIGNAT
Sbjct: 553 EWQRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNAT 612

Query: 542 DWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMA 601
           DWE+VCMGNLVGLP++VVPTGFK ISNPP+TGTTRR+T ITTGIYAPPH+DHIALALAMA
Sbjct: 613 DWEKVCMGNLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMA 672

Query: 602 YQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           YQSATDHHRA+PPIDDLGP D +P+PPLV IPPRLLHL
Sbjct: 673 YQSATDHHRAKPPIDDLGPSDVLPDPPLVSIPPRLLHL 684

BLAST of Carg21646 vs. ExPASy TrEMBL
Match: A0A1S4DX80 (glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo OX=3656 GN=LOC103490981 PE=4 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 4.3e-286
Identity = 491/591 (83.08%), Postives = 538/591 (91.03%), Query Frame = 0

Query: 51  TYKKIWKLLCEKVGVHYRSPSYRTETKTLKVSKMVE---KDLFLEHLMQTSSVT-LRYTY 110
           TYKK WKLL EKVGVH    S+  ++K  K S+ +E   +   L+ + +         T 
Sbjct: 3   TYKKFWKLLYEKVGVHKPYSSFLQDSK--KDSECLENMGRTSILKCVFRAFDADFFNNTK 62

Query: 111 MDEVLKGAEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVS 170
            DEVLKGAEE N+P IRANRKLVASENGGLHNPSPLVF+  WANE   HKS+RFCYPPV 
Sbjct: 63  RDEVLKGAEEVNVPTIRANRKLVASENGGLHNPSPLVFNPEWANENARHKSKRFCYPPVH 122

Query: 171 GVKRPKNEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAY 230
           GVKRP NE+DIAFMSVLELGELIKT+QI+SQELV IFL+RLKRY++VL+AVVSFTE+LAY
Sbjct: 123 GVKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAY 182

Query: 231 KQAKEADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLK 290
           KQAKEADELFA+GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVY+QLK
Sbjct: 183 KQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLK 242

Query: 291 SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSET 350
           SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAAC SAG+VPFAIGSET
Sbjct: 243 SAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSET 302

Query: 351 AGSMTYPASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDP 410
            GSMTYPA+RCGVTA+RPTFGTVGRSGVMSISESLDKLGPFCRNA+DCAV+LDVIRGKDP
Sbjct: 303 VGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDP 362

Query: 411 HDLSSVESFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGIL 470
           HDLSSVES LDDPFSIDISKLTVGY+DDADMEVV++LSSKG NM+PFNL+YS+DSVQGI+
Sbjct: 363 HDLSSVESSLDDPFSIDISKLTVGYVDDADMEVVQILSSKGVNMIPFNLSYSVDSVQGIV 422

Query: 471 NFTMDIDMLTHFDEWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVK 530
           NFTMD+DML HFDEWQR GLD EYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIRE++
Sbjct: 423 NFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREIR 482

Query: 531 ESFNVDALIGNATDWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAP 590
           ESFNVDALIGNATDWE+VCMGNLVGLP++VVPTGFK ISNPP+TGTTRR+  IT+GIYAP
Sbjct: 483 ESFNVDALIGNATDWEKVCMGNLVGLPIIVVPTGFKNISNPPSTGTTRRKVTITSGIYAP 542

Query: 591 PHKDHIALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           PH+DHIALALAMAYQSATDHHRARPPIDDLGP+D +P+PPLV IPPRLLHL
Sbjct: 543 PHRDHIALALAMAYQSATDHHRARPPIDDLGPNDVLPDPPLVSIPPRLLHL 591

BLAST of Carg21646 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 840.1 bits (2169), Expect = 1.2e-243
Identity = 415/587 (70.70%), Postives = 484/587 (82.45%), Query Frame = 0

Query: 53  KKIWKLLCEKVGVHYRSPSYRTETKTLK--VSKMVEKDLFLEHLMQTSSVTLRYTYMDEV 112
           K +WKLL  KV V  R  +  T T TL     K +E            S     T + E+
Sbjct: 74  KNLWKLLDRKVSVPCRKITTLTRTVTLTELPDKTMEAKCLQGMFELIDSAFFNETKLQEI 133

Query: 113 LKGAEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKR 172
            KGA E N+P+ RANRKLVA++NGGL NPSPLVF+ +W  E    + +RF YP  SGVK 
Sbjct: 134 AKGATEMNVPIYRANRKLVATKNGGLENPSPLVFNPSWNREVQRVQDKRFKYPSASGVKL 193

Query: 173 PKNEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAK 232
           P++EEDIAFMSVLELGELIKT+QITS+ELV I+LK+LKRY++VL+AVV++TE+LAYKQAK
Sbjct: 194 PRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAK 253

Query: 233 EADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGA 292
           EAD+L ++G YLGPLHGIPYGLKDI++VPGYKTTWGS SFK+Q +D+EAWVY++LK++GA
Sbjct: 254 EADDLLSQGTYLGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRLKASGA 313

Query: 293 VLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSM 352
           VLVAKLVTGS+AYDDIWFGGRTRNPWNIEEF+TGSSAGPAA  SAG+VPFAIGSETAGSM
Sbjct: 314 VLVAKLVTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSM 373

Query: 353 TYPASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLS 412
           TYPA+RCG+TALRPTFG+VGR+GVMSISESLDKLGPFCR A DCAVILD I+GKDP DLS
Sbjct: 374 TYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLS 433

Query: 413 SVESFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTM 472
           S E   +DPFS+DI+KLTVGY  DADM+VV VL SKG NMVPF L Y+++SVQGILNFTM
Sbjct: 434 SREIAFEDPFSVDITKLTVGYTKDADMKVVEVLGSKGVNMVPFELNYTVESVQGILNFTM 493

Query: 473 DIDMLTHFDEWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFN 532
           D+DML HFDEWQR+G +  YEAQDQWP ELRRAR++ AVDY+QAQRARGKLIREV++SF 
Sbjct: 494 DVDMLAHFDEWQRTGQEDLYEAQDQWPVELRRARVVTAVDYIQAQRARGKLIREVEKSFT 553

Query: 533 VDALIGNATDWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKD 592
           VDA IGN TDWE+VCMGNLVGLPV+V+PTGFK I++PP T + RRRT I  GIYAPP +D
Sbjct: 554 VDAFIGNVTDWEKVCMGNLVGLPVLVIPTGFKNITDPP-TNSCRRRTTINAGIYAPPERD 613

Query: 593 HIALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           HIALAL MAYQS TD HR RPPIDDLGP D+IP PP   IPPR LH+
Sbjct: 614 HIALALGMAYQSVTDAHRKRPPIDDLGPDDSIPNPPRALIPPRRLHI 659

BLAST of Carg21646 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 820.8 bits (2119), Expect = 7.3e-238
Identity = 409/587 (69.68%), Postives = 477/587 (81.26%), Query Frame = 0

Query: 53  KKIWKLLCEKVGVHYRSPSYRTETKTLK--VSKMVEKDLFLEHLMQTSSVTLRYTYMDEV 112
           K +WKLL  KV V  R  +  T T TL     K +E            S     T + E+
Sbjct: 74  KNLWKLLDRKVSVPCRKITTLTRTVTLTELPDKTMEAKCLQGMFELIDSAFFNETKLQEI 133

Query: 113 LKGAEEFNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKR 172
            KGA E N+P+ RANRKLVA++NGGL NPSPLVF+ +W  E    + +RF YP  SGVK 
Sbjct: 134 AKGATEMNVPIYRANRKLVATKNGGLENPSPLVFNPSWNREVQRVQDKRFKYPSASGVKL 193

Query: 173 PKNEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAK 232
           P++EEDIAFMSVLELGELIKT+QITS+ELV I+LK+LKRY++VL+AVV++TE+LAYKQAK
Sbjct: 194 PRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAK 253

Query: 233 EADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGA 292
           EAD+L ++G YLGPLHGIPYGLKDI++VPGYKTTWGS SFK+Q +D+EAWVY++LK++GA
Sbjct: 254 EADDLLSQGTYLGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRLKASGA 313

Query: 293 VLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSM 352
           VLVAKLVTGS+AYDDIWFGGRTRNPWNIEEF+TGSSAGPAA  SA       GSETAGSM
Sbjct: 314 VLVAKLVTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSM 373

Query: 353 TYPASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLS 412
           TYPA+RCG+TALRPTFG+VGR+GVMSISESLDKLGPFCR A DCAVILD I+GKDP DLS
Sbjct: 374 TYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLS 433

Query: 413 SVESFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTM 472
           S E   +DPFS+DI+KLTVGY  DADM+VV VL SKG NMVPF L Y+++SVQGILNFTM
Sbjct: 434 SREIAFEDPFSVDITKLTVGYTKDADMKVVEVLGSKGVNMVPFELNYTVESVQGILNFTM 493

Query: 473 DIDMLTHFDEWQRSGLDHEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVKESFN 532
           D+DML HFDEWQR+G +  YEAQDQWP ELRRAR++ AVDY+QAQRARGKLIREV++SF 
Sbjct: 494 DVDMLAHFDEWQRTGQEDLYEAQDQWPVELRRARVVTAVDYIQAQRARGKLIREVEKSFT 553

Query: 533 VDALIGNATDWERVCMGNLVGLPVMVVPTGFKIISNPPATGTTRRRTAITTGIYAPPHKD 592
           VDA IGN TDWE+VCMGNLVGLPV+V+PTGFK I++PP T + RRRT I  GIYAPP +D
Sbjct: 554 VDAFIGNVTDWEKVCMGNLVGLPVLVIPTGFKNITDPP-TNSCRRRTTINAGIYAPPERD 613

Query: 593 HIALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 638
           HIALAL MAYQS TD HR RPPIDDLGP D+IP PP   IPPR LH+
Sbjct: 614 HIALALGMAYQSVTDAHRKRPPIDDLGPDDSIPNPPRALIPPRRLHI 652

BLAST of Carg21646 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 120.6 bits (301), Expect = 4.7e-27
Identity = 77/243 (31.69%), Postives = 122/243 (50.21%), Query Frame = 0

Query: 193 QITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGVYLGPLHGIPYGL 252
           + T+ E+   +L R++  +  L+  +  +E++  K A+E D+  A+G  LGPL G+  G+
Sbjct: 57  ETTAVEIAKSYLSRIRLTEPQLKCFLHVSENV-LKDAQEIDQRIAKGEELGPLAGVLIGV 116

Query: 253 KDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGSLAYDDIWFGGRT 312
           KD I   G  +T  S+  +      +A   +++K  G ++V     G    D+   G  T
Sbjct: 117 KDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVV-----GKTNMDEFGMGSTT 176

Query: 313 R--------NPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGVTALRP 372
                    NPW++     GSS G AA ++A     ++GS+T GS+  PAS CGV  L+P
Sbjct: 177 EASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKP 236

Query: 373 TFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSS----VESFLDDPF 424
           T+G V R G+M+ + SLD +G F     D  ++L  I G D  D +S    V  F     
Sbjct: 237 TYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPEFQSQFL 293

BLAST of Carg21646 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 90.5 bits (223), Expect = 5.2e-18
Identity = 57/198 (28.79%), Postives = 103/198 (52.02%), Query Frame = 0

Query: 217 VVSFTEDLAYKQAKEADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKE-QVI 276
           ++ F  +   KQA+ +   F +G  +  L GI   +KD I    + T  G+    E + +
Sbjct: 169 LIRFDANEVIKQAEASTRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSV 228

Query: 277 DVEAWVYQQLKSAGAVLVAK-----LVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPA 336
           + ++ V  +L+S GA+L+ K     L  G+   +  +  G TRNP + + +T GSS+G A
Sbjct: 229 EKDSAVVSKLRSCGAILLGKANMHELGMGTTGNNSNY--GTTRNPHDPKRYTGGSSSGSA 288

Query: 337 ACISAGLVPFAIGSETAGSMTYPASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRN 396
           A ++AGL   A+G++  GS+  P++ CG+T L+ T+G    +G +    +++ +GP   +
Sbjct: 289 AIVAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASS 348

Query: 397 AIDCAVILDVIRGKDPHD 409
             D  ++   I G    D
Sbjct: 349 LEDAFLVYAAILGSSSAD 364

BLAST of Carg21646 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 82.8 bits (203), Expect = 1.1e-15
Identity = 50/151 (33.11%), Postives = 76/151 (50.33%), Query Frame = 0

Query: 245 LHGIPYGLKDIISVPGYKTTWGSKSF--KEQVIDVEAWVYQQLKSAGAVLVAKLVTGSLA 304
           L G+ + +KDI  V G  T +G+  +          A V   L  AGA  +   +   +A
Sbjct: 28  LQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPVVSSLLEAGATALGITIMDEMA 87

Query: 305 YD---DIWFGGRTRNPWNIEEFTTGSSAGPAACISAGLVPFAIGSETAGSMTYPASRCGV 364
           Y    +    G  RNP   +    GSS+G A  ++A LV F+IG++T GS+  PAS CG+
Sbjct: 88  YSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIGTDTGGSVRVPASYCGI 147

Query: 365 TALRPTFGTVGRSGVMSISESLDKLGPFCRN 391
              RP+ G V   G+  +++S D +G F R+
Sbjct: 148 FGFRPSHGAVSTVGLTPMAQSFDTVGWFARD 178

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028327.10.0e+00100.00gatA, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022946706.10.0e+0093.48uncharacterized protein LOC111450695 [Cucurbita moschata][more]
XP_023540352.10.0e+0092.87uncharacterized protein LOC111800751 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023005329.10.0e+0092.79uncharacterized protein LOC111498360 isoform X1 [Cucurbita maxima][more]
KAG6596801.10.0e+0091.68hypothetical protein SDJN03_09981, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q3AD368.9e-4729.92Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Carboxydothermus hydrogenoforma... [more]
B2V8552.7e-4328.14Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Sulfurihydrogenibium sp. (strai... [more]
Q2RGY43.5e-4338.27Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Moorella thermoacetica (strain ... [more]
A5UXF98.7e-4230.22Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Roseiflexus sp. (strain RS-1) O... [more]
P634971.5e-4129.23Putative amidase AmiD OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) O... [more]
Match NameE-valueIdentityDescription
A0A6J1G4E40.0e+0093.48uncharacterized protein LOC111450695 OS=Cucurbita moschata OX=3662 GN=LOC1114506... [more]
A0A6J1KYV30.0e+0092.79uncharacterized protein LOC111498360 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1KX470.0e+0089.01uncharacterized protein LOC111498360 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0L5U77.8e-28880.09Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G039330 PE=... [more]
A0A1S4DX804.3e-28683.08glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT5G07360.11.2e-24370.70Amidase family protein [more]
AT5G07360.27.3e-23869.68Amidase family protein [more]
AT3G25660.14.7e-2731.69Amidase family protein [more]
AT5G64440.15.2e-1828.79fatty acid amide hydrolase [more]
AT1G08980.11.1e-1533.11amidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 177..613
e-value: 1.4E-94
score: 319.8
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 156..604
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 198..535
e-value: 8.4E-58
score: 196.5
IPR000120AmidasePANTHERPTHR11895TRANSAMIDASEcoord: 82..611
NoneNo IPR availablePANTHERPTHR11895:SF73AMIDASE FAMILY PROTEINcoord: 82..611

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21646-RACarg21646-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016740 transferase activity
molecular_function GO:0003824 catalytic activity