Carg21615 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21615
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Non-specific serine/threonine protein kinase
Location	Carg_Chr06: 3974089 .. 3977968 (+)
RNA-Seq Expression	Carg21615
Synteny	Carg21615

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTTGACGTTAACGTGTTGCGCTATCTCTCGAAAGATGATTTTAGGGTTCTTACTGCTGTTGAGATGGGGATGCGGAATGTATGTACTGATTTTCTCTTCTCTAGAGGGAACTTTAACTCATCATTTTAAGTTTATTTTGTTCCTTGAACCTTACTCTAATGTAATATCTTTTGTTGTTTCAGCATGAAATTGTTCCTTCAGAACTCGTGGAAAGTATAGCTTCTCTTAAGTATGACTTCAATTCTGATATTGTCTAGAAATATTCTTTCCCTTGCTTCTAGTAAATGTCACGTCCTACTATGGTGTGTGTAGGCATCGAGGCACTTACAAAGTTTTGAAGAATTTACTCAAATATAAGTTATTGCACCACGACTCTTCTAAATGTGAGTAGCTATTAACTTATGGTATGGGAATTCTAAATTTTAAACAATCTATCTCATTATCTTTCTTGTGTTCTTCTAGATGACGGGTTTCGACTTACATACCTTGGCTATGATTTTCTCGCAATTAAAACTTTGGTCAACTGTGGAGTGTTTGTGGCTGTAGGTCGACAAATTGGTGTTGGGAAAGAGTCAGGTAAATTTCTAGCTAGTTAGAGCTTTAAAAGTTGTTTCTATTTGTCCTGTTATTATTCATTGGCTGGGTTGCTATTGTTGATTATAGATATTTTTGAGGTTGCAAAAGAAGATGGCACTGTGCTTGCCATGAAGTTGCATAGACTTGGTAGAATTTCTTTTAGGTCGGTTAAATCTAAGCGTGATTATTTGAGGCATCGCAGTAGTTATAATTGGCTTTACTTGTCTCGGCTTGCGGCGTTTAAAGAATATGCATTCATGAAGGTGCTCTGTTGTATCGATTTTGTATTGGCATTGTATTTACGTATTTTAGTAGAATATGCATTCATGAAGGTGCTCTGTTGTATCGATTTTGTATTGGCATTGTATTTACGTATTTTAGTGAATTGTGATATTGATGGAGGCTTCATTGGTGATAATAGGCTTTGGAAGAACATGGTTTTCCTGTTCCAAATGCAGTAGACTGTAATAGGCATTGTGTCGTTATGTCTCTCGTTGAAGGATATCCGCTGTACGTCTTTCCTTCTTGGTTGAGTAGGGTCATGAAAGATTTTGATGAGCACGTATGCTTTAACGAATATTATGATTCTATGTCGTACCAATTCGTTCCTTCTTGGTTTATTGCAGTGTGCAGGTAAAACAATTACAAAATCCAGATGTGGTTTTTGAAACAATCATTGGTCTTGTTGTTCGACTAGCGGAACACGGACTCATTCATTGTGACTTTAACGAATTCAACATTATGGTATTCTAAGAAACAATGCTATTTACTGTTCTATTATTGTCCCTTTTCCTCGCTTAAGATGATGTCTTTTCCTCAACTTAATATATGATGTCTCTTTCTCGTCTTCATTCAATGAAACAAATTATTGCTGTTTCTCGTAAAAGGGTATTCGTGATCGTTTGTTTTTTTGAACTTACAAATATCTAATTGTAGTTCAAGTTCTCTTTTGAGCTTACCACCGTTTATTCAATCTTTCTGTTCTTGTGTTTCATAGATTGATGATGATGAGAAGGTCACAATGATCGATTTTCCACAAATGGTTTCTGTCTCGCATCGTAATGCACAAATGTAAGCGGTGCAAATATTGATTTTTCGGGTTACAATGTTTCTCGTATCATATGAATCCTAAAGTTCATGTTTCTATTAGCTTATGCTTTACGTGACGATCTTTTTAAGTTTCGTTTTTCCATTTCAGGTATTTTGATCGTGATGTTGAATGCATCTTCAAATTTTTTAGGAAAAGGTCAGTGTTGTTGTAAGAAACAATAAGAATGTTTGATGTTACAAGAAGTTGAAACTTTCATGTTACATACTTCTCATGGAGTTTCTCTTCAGCTAATTTGCATTCAAGATTGAAAATTCTAAAGAACAATATAATACTGCTTTTCGAGATCGTGCAGTTATGGTCTCTGTGTTCTGGTTGCAACTTTTTACGTTCCGTTCTTAACTGGACACGATTTGTGAGGTTTAGATTAGATCGTAGCAAGTTTAATCGAAGTATGTGAACGACATATTAGAACTCGACTAGTAGGTTTGGCTGTTATCTTGAGGTGTAGCACATTATTGATATGCTAAAGACTTTCAGTTCCTTTCTCTTACAGGTTTAACATGTCATTCCATGAGACCGTGGATGACGAAAATGATGTAGAGATCGACAATGATGAAGGTGCGAGGCCTTGCTTTTCTTCGATATCGAAAGGTGCTGGAAGTCTAGACAAGGAGCTTGCTGCCAGTGGTTAACCAGTTCATTCTAAACTTATTGGCGATTGTACGACATGCAGTTTATTGAAGGTGGAATTGAGGGAGATGCAGATTCAGGCGATGAAGAAGCTGAAAGCTTCAAGTTAGATGAAATGGACACCAATGTGGTGGAACAACATCTACATGTGTCAGACGAGGTATTCATCTCATCATTCTGATTCGAACCGATCCATTGGTAGCAGATATTGTCCTCTTTGAGCTTTCCCTTCAAGGTTTTTAAAATGTATTTGCTAGGGAGAAATTTTCACACCCTTAATGCTTCGTTCTCCTCAACCACAATCCACCCCCTTCGGAGTCCAGCATCCTCGTTGACACTTGTTCTCTTCTCCGATCGATGTGGGACCTCCTAATCCACTCCCTTCGGGCCCAACATCATTGCTGACACACCACCTTGTGTCCACCCACCTTTGGGGCTTAGCCTCCTTGCTGGCACATCACCCAGTGTTTGGCTTTGATACTATTTGTAACAGCCAGAGCCCATTGCTAGTACATATTGTCCTCTTTGGGCTTTCCCTTCCAAGCTTCCTCTCAAAATTTTTAAAATGCGTCTGCTGTGAAGAGGTTTCTACACCCTTATAAAGAAAACTTCGTTCTCCTTCCCAACCGATGTGAGATCTCAGAATCCACCCACTTCGAAGCCCAGCGTCCTCGCTAGCCCTGGTTCCCTTCTCCAATCGATATGGGAACCCCCAATCCACCCCTTCGGAGCCCAACATCCTTGCTGGCACACTGCCTTGTGTCCACCTCCTTCGAAGCTCAGTCTCCTCATTGACACACTGCTCGGTGTTTGACTCTGATACTATTCGTTACAACTCAATCCCACTCTTAACAAATATTGTCATCTTTGAGCTTTCTCTTCCGGGCTTCCCCTCAAGGTTTTTAAAATTCATCTGATATGGAGAGGTTTCCACACCCTTATCCCACATTGGTTGGGGAGGAGAACGAAACACCCTTTGTAAGGGTGTGGAAACCTTCCTAGCAGACGCGTTTTAAAGCCTTGAGGGGAAGCCTAAAGAGGACAATATCTACTAGTGGTGAATTTGGGCCGTTACAACTTCGTTTTCCTCACCAACTGACGTGGGATCTCGCAATCTTTCTTAACAACTTGAGCTCTATATAATGGATGGCACTGTTTGTTTGTTGAGAATGTAGCTGATGTACATCTATTTCCCTTTTAATTGTAATTCACAGGAAACCTTTAACTGTGATGAAAGTGCAGATTGTCAGAGGAACGAAGATTCAGATGTATTGTTTCTAACCTTGTTGGTTCGCTAATAGTTTGGTGAGCAAAGGTAACAGTTGGTATGTGTTTGTGTTTTTTCCATTGCAGAGGGAGAGTGATGGTGAGGGAAGGGATGAAAGTGATGACACAGAACTGAACAGACGGTTGAACAAGCAGAGACAACGGGCCATAGCATCAGCCAGTCGCGGTGGAGGAAGAAAGGGTCCTGCCTCCAGAAACAACACCTACAAAGACAAGGGTAGGAAGTCTTCTAACAATTCCAAACTTCATAAACAAATCATCATGTGGTGA

mRNA sequence

ATGAAGCTTGACGTTAACGTGTTGCGCTATCTCTCGAAAGATGATTTTAGGGTTCTTACTGCTGTTGAGATGGGGATGCGGAATCATGAAATTGTTCCTTCAGAACTCGTGGAAAATGACGGGTTTCGACTTACATACCTTGGCTATGATTTTCTCGCAATTAAAACTTTGGTCAACTGTGGAGTGTTTGTGGCTGTAGGTCGACAAATTGGTGTTGGGAAAGAGTCAGATATTTTTGAGGTTGCAAAAGAAGATGGCACTGTGCTTGCCATGAAGTTGCATAGACTTGGTAGAATTTCTTTTAGGTCGGTTAAATCTAAGCGTGATTATTTGAGGCATCGCAGTAGTTATAATTGGCTTTACTTGTCTCGGCTTGCGGCGTTTAAAGAATATGCATTCATGAAGGTAAAACAATTACAAAATCCAGATGTGGTTTTTGAAACAATCATTGGTCTTGTTGTTCGACTAGCGGAACACGGACTCATTCATTGTGACTTTAACGAATTCAACATTATGATTGATGATGATGAGAAGGTCACAATGATCGATTTTCCACAAATGGTTTCTGTCTCGCATCGTAATGCACAAATGTATTTTGATCGTGATGTTGAATGCATCTTCAAATTTTTTAGGAAAAGGTTTAACATGTCATTCCATGAGACCGTGGATGACGAAAATGATGTAGAGATCGACAATGATGAAGGTGCGAGGCCTTGCTTTTCTTCGATATCGAAAGGTGCTGGAAGTCTAGACAAGGAGCTTGCTGCCAGTGATTGTCAGAGGAACGAAGATTCAGATAGGGAGAGTGATGGTGAGGGAAGGGATGAAAGTGATGACACAGAACTGAACAGACGGTTGAACAAGCAGAGACAACGGGCCATAGCATCAGCCAGTCGCGGTGGAGGAAGAAAGGGTCCTGCCTCCAGAAACAACACCTACAAAGACAAGGGTAGGAAGTCTTCTAACAATTCCAAACTTCATAAACAAATCATCATGTGGTGA

Coding sequence (CDS)

ATGAAGCTTGACGTTAACGTGTTGCGCTATCTCTCGAAAGATGATTTTAGGGTTCTTACTGCTGTTGAGATGGGGATGCGGAATCATGAAATTGTTCCTTCAGAACTCGTGGAAAATGACGGGTTTCGACTTACATACCTTGGCTATGATTTTCTCGCAATTAAAACTTTGGTCAACTGTGGAGTGTTTGTGGCTGTAGGTCGACAAATTGGTGTTGGGAAAGAGTCAGATATTTTTGAGGTTGCAAAAGAAGATGGCACTGTGCTTGCCATGAAGTTGCATAGACTTGGTAGAATTTCTTTTAGGTCGGTTAAATCTAAGCGTGATTATTTGAGGCATCGCAGTAGTTATAATTGGCTTTACTTGTCTCGGCTTGCGGCGTTTAAAGAATATGCATTCATGAAGGTAAAACAATTACAAAATCCAGATGTGGTTTTTGAAACAATCATTGGTCTTGTTGTTCGACTAGCGGAACACGGACTCATTCATTGTGACTTTAACGAATTCAACATTATGATTGATGATGATGAGAAGGTCACAATGATCGATTTTCCACAAATGGTTTCTGTCTCGCATCGTAATGCACAAATGTATTTTGATCGTGATGTTGAATGCATCTTCAAATTTTTTAGGAAAAGGTTTAACATGTCATTCCATGAGACCGTGGATGACGAAAATGATGTAGAGATCGACAATGATGAAGGTGCGAGGCCTTGCTTTTCTTCGATATCGAAAGGTGCTGGAAGTCTAGACAAGGAGCTTGCTGCCAGTGATTGTCAGAGGAACGAAGATTCAGATAGGGAGAGTGATGGTGAGGGAAGGGATGAAAGTGATGACACAGAACTGAACAGACGGTTGAACAAGCAGAGACAACGGGCCATAGCATCAGCCAGTCGCGGTGGAGGAAGAAAGGGTCCTGCCTCCAGAAACAACACCTACAAAGACAAGGGTAGGAAGTCTTCTAACAATTCCAAACTTCATAAACAAATCATCATGTGGTGA

Protein sequence

MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVENDGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAMKLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKVTMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPCFSSISKGAGSLDKELAASDCQRNEDSDRESDGEGRDESDDTELNRRLNKQRQRAIASASRGGGRKGPASRNNTYKDKGRKSSNNSKLHKQIIMW

Homology

[Close]

BLAST of Carg21615 vs. NCBI nr
Match: KAG7028358.1 (Serine/threonine-protein kinase rio2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652.1 bits (1681), Expect = 2.5e-183
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVENDGFRLTYLGYDFLAIKTLVNC 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVENDGFRLTYLGYDFLAIKTLVNC
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVENDGFRLTYLGYDFLAIKTLVNC 60

Query: 61  GVFVAVGRQIGVGKESDIFEVAKEDGTVLAMKLHRLGRISFRSVKSKRDYLRHRSSYNWL 120
           GVFVAVGRQIGVGKESDIFEVAKEDGTVLAMKLHRLGRISFRSVKSKRDYLRHRSSYNWL
Sbjct: 61  GVFVAVGRQIGVGKESDIFEVAKEDGTVLAMKLHRLGRISFRSVKSKRDYLRHRSSYNWL 120

Query: 121 YLSRLAAFKEYAFMKVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKVT 180
           YLSRLAAFKEYAFMKVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKVT
Sbjct: 121 YLSRLAAFKEYAFMKVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKVT 180

Query: 181 MIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPCF 240
           MIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPCF
Sbjct: 181 MIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPCF 240

Query: 241 SSISKGAGSLDKELAASDCQRNEDSDRESDGEGRDESDDTELNRRLNKQRQRAIASASRG 300
           SSISKGAGSLDKELAASDCQRNEDSDRESDGEGRDESDDTELNRRLNKQRQRAIASASRG
Sbjct: 241 SSISKGAGSLDKELAASDCQRNEDSDRESDGEGRDESDDTELNRRLNKQRQRAIASASRG 300

Query: 301 GGRKGPASRNNTYKDKGRKSSNNSKLHKQIIMW 334
           GGRKGPASRNNTYKDKGRKSSNNSKLHKQIIMW
Sbjct: 301 GGRKGPASRNNTYKDKGRKSSNNSKLHKQIIMW 333

[Close]

BLAST of Carg21615 vs. NCBI nr
Match: XP_023541015.1 (serine/threonine-protein kinase rio2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 569.7 bits (1467), Expect = 1.7e-158
Identity = 326/454 (71.81%), Postives = 327/454 (72.03%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLKHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMKALEEHGFPVPNAVDCN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV
Sbjct: 181 RHCVVMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFN+SFHETVDDENDVEIDNDEGARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNVSFHETVDDENDVEIDNDEGARPC 300

Query: 301 FSSISKGAGSLDKELAAS------------------------------------------ 334
           F SISKGAGSLDKELAAS                                          
Sbjct: 301 FCSISKGAGSLDKELAASGFTRKDQEDIEKFIEGGIEGDADSGDEEAESFKFDEMDTNVV 360

[Close]

BLAST of Carg21615 vs. NCBI nr
Match: XP_023553068.1 (serine/threonine-protein kinase rio2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 517.3 bits (1331), Expect = 9.7e-143
Identity = 306/482 (63.49%), Postives = 316/482 (65.56%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVEC+FKFFRKRFN+SFHETVDDENDVEIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDVEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASDCQRNEDSD--------------------------------- 334
           FSSISKGAGSLDKELAAS   R E  D                                 
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGEEEAESSKLNEMDINAV 360

[Close]

BLAST of Carg21615 vs. NCBI nr
Match: XP_023007804.1 (serine/threonine-protein kinase rio2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 517.3 bits (1331), Expect = 9.7e-143
Identity = 306/482 (63.49%), Postives = 316/482 (65.56%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVEC+FKFFRKRFN+SFHETVDDEND+EIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDLEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASDCQRNEDSD--------------------------------- 334
           FSSISKGAGSLDKELAAS   R E  D                                 
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGDEEAESSKLNEMDTNAV 360

[Close]

BLAST of Carg21615 vs. NCBI nr
Match: XP_022923288.1 (serine/threonine-protein kinase rio2 isoform X2 [Cucurbita moschata] >KAG6577587.1 Serine/threonine-protein kinase rio2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 517.3 bits (1331), Expect = 9.7e-143
Identity = 306/482 (63.49%), Postives = 316/482 (65.56%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVEC+FKFFRKRFN+SFHETVDDENDVEIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDVEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASDCQRNEDSD--------------------------------- 334
           FSSISKGAGSLDKELAAS   R E  D                                 
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGEEEAESSKLNEMDTNAV 360

[Close]

BLAST of Carg21615 vs. ExPASy Swiss-Prot
Match: Q54T05 (Serine/threonine-protein kinase rio2 OS=Dictyostelium discoideum OX=44689 GN=rio2 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 164/371 (44.20%), Postives = 216/371 (58.22%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELV----------------------- 60
           MKLD   LRYLSKDDFR L AVEMGM+NHE+VP  L+                       
Sbjct: 1   MKLDPKALRYLSKDDFRTLVAVEMGMKNHELVPVSLICTIANLKYGGTKKSIQTLHKFKL 60

Query: 61  ------ENDGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DG++LTYLGYDFLA+KTLV+ GV   VG QIGVGKESDI+ VA +D   + +
Sbjct: 61  LFHDGRNYDGYKLTYLGYDFLALKTLVSRGVCSYVGNQIGVGKESDIYIVANDDNQEMVL 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGR+SF+++K+ RDYL+HR S +WLYLSRLAA KE+A+MK                
Sbjct: 121 KLHRLGRVSFKTIKNNRDYLKHRKSASWLYLSRLAALKEFAYMKALYENGFPVPTPIDYN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           + QL++P  V+  ++ L+V+LA +GLIH DFNEFNI+I+D+E++
Sbjct: 181 RHCIVMSRARGYPLTQIVQLRHPSKVYSDLMNLIVKLASYGLIHGDFNEFNILINDEEEI 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNM---SFHETVDDEN----DVEIDN 300
           T+IDFPQMVS SH NA+MYFDRDV CI  FF KRFN     + +  D +N    DVEI  
Sbjct: 241 TLIDFPQMVSTSHLNAEMYFDRDVTCIRVFFEKRFNFIGERWPKFKDCQNIHSLDVEISA 300

[Close]

BLAST of Carg21615 vs. ExPASy Swiss-Prot
Match: Q9CQS5 (Serine/threonine-protein kinase RIO2 OS=Mus musculus OX=10090 GN=Riok2 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.2e-64
Identity = 145/329 (44.07%), Postives = 191/329 (58.05%), Query Frame = 0


Query: 2   KLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN---------------------- 61
           K++V  LRY+S+DDFRVLTAVEMGM+NHEIVP  L+ +                      
Sbjct: 3   KVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPCSLIASIASLKHGGCNKILRELVKHKLI 62

Query: 62  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 121
                    G+RLT  GYD+LA+KTL +  V  +VG Q+GVGKESDI+ VA E G  LA+
Sbjct: 63  AWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEAGQQLAL 122

Query: 122 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 181
           KLHRLGR SFR++K+KRDY +HR + +WLYLSRL+A KE+A+MK                
Sbjct: 123 KLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYN 182

Query: 182 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 241
                           +  +++P  V++  + L+V+L  HGLIH DFNEFN+M+D D+ +
Sbjct: 183 RHAVIMELINGYPLCQIHHVEDPASVYDEAMELIVKLGNHGLIHGDFNEFNLMLDKDDHI 242

Query: 242 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 269
           TMIDFPQMVS SH NA+ YFDRDV+CI +FF KRF+                 +    P 
Sbjct: 243 TMIDFPQMVSTSHPNAEWYFDRDVKCIREFFMKRFSY----------------ESELYPT 302

[Close]

BLAST of Carg21615 vs. ExPASy Swiss-Prot
Match: Q9BVS4 (Serine/threonine-protein kinase RIO2 OS=Homo sapiens OX=9606 GN=RIOK2 PE=1 SV=2)

HSP 1 Score: 240.7 bits (613), Expect = 2.3e-62
Identity = 140/329 (42.55%), Postives = 190/329 (57.75%), Query Frame = 0


Query: 2   KLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN---------------------- 61
           K++V  LRY+S+DDFRVLTAVEMGM+NHEIVP  L+ +                      
Sbjct: 3   KVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLI 62

Query: 62  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 121
                    G+RLT  GYD+LA+KTL +  V  +VG Q+GVGKESDI+ VA E+G   A+
Sbjct: 63  AWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFAL 122

Query: 122 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 181
           KLHRLGR SFR++K+KRDY +HR + +WLYLSRL+A KE+A+MK                
Sbjct: 123 KLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYN 182

Query: 182 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 241
                           +  +++P  V++  + L+V+LA HGLIH DFNEFN+++D+ + +
Sbjct: 183 RHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHI 242

Query: 242 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 269
           TMIDFPQMVS SH NA+ YFDRDV+CI  FF KRF+                 +    P 
Sbjct: 243 TMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSY----------------ESELFPT 302

[Close]

BLAST of Carg21615 vs. ExPASy Swiss-Prot
Match: Q9P7W5 (Serine/threonine-protein kinase rio2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rio2 PE=3 SV=2)

HSP 1 Score: 222.2 bits (565), Expect = 8.5e-57
Identity = 132/344 (38.37%), Postives = 191/344 (55.52%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           + L++  +RYLS +DFR LTAVEMG RNHE+VP+ ++                       
Sbjct: 2   VNLNIKAMRYLSAEDFRTLTAVEMGSRNHEVVPTNMINQIAKIRGGSCTKSLSVLFMHKL 61

Query: 61  ---------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLA 120
                    +G+RLTY GYD+LA+K L       +VG QIGVGKESD++ V  + G    
Sbjct: 62  IAKVPHISYEGYRLTYAGYDYLALKALSKRASVYSVGNQIGVGKESDVYVVGDQKGKQYI 121

Query: 121 MKLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK--------------- 180
           +K+HRLGRISFRSVK+ RDYLR+R + +W YLSRLAA KE+AFMK               
Sbjct: 122 LKIHRLGRISFRSVKNNRDYLRNRKTGSWQYLSRLAATKEFAFMKILHEHGFPVPAPIDH 181

Query: 181 -----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEK 240
                            V  +++P  +++T++ ++VR A +GLIH DFNEFNI++ +D  
Sbjct: 182 SRHCIIMEMIDAFPLRAVTDIRDPPALYQTLMDIIVRFARNGLIHGDFNEFNIIVREDGT 241

Query: 241 VTMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFH-ETVDDENDVEIDNDEGAR 282
             +IDFPQMVS SH +AQ YFDRDV+CI ++F K +         +D + +E +N+    
Sbjct: 242 AVVIDFPQMVSTSHPDAQFYFDRDVQCIVQYFEKNYQYKGDVPNFEDISKMEKENN---- 301

[Close]

BLAST of Carg21615 vs. ExPASy Swiss-Prot
Match: Q95Q34 (Serine/threonine-protein kinase RIO2 OS=Caenorhabditis elegans OX=6239 GN=riok-2 PE=2 SV=2)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 128/347 (36.89%), Postives = 189/347 (54.47%), Query Frame = 0


Query: 2   KLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELV------------------------ 61
           +++V+++RYL  D FRVL AVEMGM+NHE+VP  LV                        
Sbjct: 3   RMNVSMMRYLEGDHFRVLIAVEMGMKNHEVVPLALVSAIAGIHRGGVARTLNDLCKHSLV 62

Query: 62  ------ENDGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 121
                 + DG+RLT  GYD+LA++ L +  V  +VG QIG+GKESD++     +   L +
Sbjct: 63  AFERSKKFDGYRLTIRGYDYLALRALCSREVVGSVGNQIGIGKESDVYVGGDPELNDLCL 122

Query: 122 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 181
           K HRLGR SFR +K KRDY + R S +WLYLSRLAA KE+AF+K                
Sbjct: 123 KFHRLGRTSFRKIKEKRDYHKKRKSASWLYLSRLAAAKEFAFLKALQERGFPVPKAVDVC 182

Query: 182 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 241
                           V  +++   +++ ++ L+V++A HG+IH DFNEFN+++ +DE++
Sbjct: 183 RHLVVMQLVVGQTLCNVTHVEDAGALYDRLMALIVKMARHGVIHGDFNEFNLIMLEDERI 242

Query: 242 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 282
            MIDFPQMVS+ H NA+ YFDRDV C+  FF+++F+    +    +      N +     
Sbjct: 243 VMIDFPQMVSIDHPNAEYYFDRDVTCVRTFFKRKFDYESEDWPKFDEVERKGNMDVLLEA 302

[Close]

BLAST of Carg21615 vs. ExPASy TrEMBL
Match: A0A6J1L5Z7 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111500302 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.7e-143
Identity = 306/482 (63.49%), Postives = 316/482 (65.56%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVEC+FKFFRKRFN+SFHETVDDEND+EIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDLEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASDCQRNEDSD--------------------------------- 334
           FSSISKGAGSLDKELAAS   R E  D                                 
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGDEEAESSKLNEMDTNAV 360

[Close]

BLAST of Carg21615 vs. ExPASy TrEMBL
Match: A0A6J1E945 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111431029 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.7e-143
Identity = 306/482 (63.49%), Postives = 316/482 (65.56%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVEC+FKFFRKRFN+SFHETVDDENDVEIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDVEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASDCQRNEDSD--------------------------------- 334
           FSSISKGAGSLDKELAAS   R E  D                                 
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGEEEAESSKLNEMDTNAV 360

[Close]

BLAST of Carg21615 vs. ExPASy TrEMBL
Match: A0A6J1L8N7 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111500302 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.2e-143
Identity = 306/483 (63.35%), Postives = 316/483 (65.42%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVEC+FKFFRKRFN+SFHETVDDEND+EIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDLEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASDCQRNEDSD--------------------------------- 334
           FSSISKGAGSLDKELAAS   R E  D                                 
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGDEEAESSKLNEMDTNAV 360

[Close]

BLAST of Carg21615 vs. ExPASy TrEMBL
Match: A0A6J1E5R3 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111431029 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.2e-143
Identity = 306/483 (63.35%), Postives = 316/483 (65.42%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLRHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPNAVDSN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMI+DDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMINDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVEC+FKFFRKRFN+SFHETVDDENDVEIDNDE ARPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECVFKFFRKRFNLSFHETVDDENDVEIDNDESARPC 300

Query: 301 FSSISKGAGSLDKELAASDCQRNEDSD--------------------------------- 334
           FSSISKGAGSLDKELAAS   R E  D                                 
Sbjct: 301 FSSISKGAGSLDKELAASGFTRKEQEDIEKFIEGGLEEDADSGEEEAESSKLNEMDTNAV 360

[Close]

BLAST of Carg21615 vs. ExPASy TrEMBL
Match: A0A0A0L5Q5 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_3G036530 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 4.1e-139
Identity = 298/479 (62.21%), Postives = 311/479 (64.93%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVN LRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNALRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIASLKHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN GVFVAVGRQIGVGKESDIFEVAKEDGTVLAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAVKEFAFMKALEEHGFPVPSAVDCN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV
Sbjct: 181 RHCVIMSLVEGYPLVQVKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFN+SFHETVDDENDVEIDNDE  RPC
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNLSFHETVDDENDVEIDNDESVRPC 300

Query: 301 FSSISKGAGSLDKELAASDCQRNEDSD--------------------------------- 334
           FSSISKG GSLDKELAAS   R +  D                                 
Sbjct: 301 FSSISKGVGSLDKELAASGFTRKDQEDIEKFIENGLEGDADSGGEEDETEDTDGVEVEHL 360

[Close]

BLAST of Carg21615 vs. TAIR 10
Match: AT3G51270.2 (protein serine/threonine kinases;ATP binding;catalytics )

HSP 1 Score: 409.1 bits (1050), Expect = 3.5e-114
Identity = 248/461 (53.80%), Postives = 278/461 (60.30%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIACLKHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN G+F  VGRQIGVGKESDIFEVA+EDGT+LAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGIFTGVGRQIGVGKESDIFEVAQEDGTILAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGR SFR+VKSKRDYLRHRSS++WLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRTSFRAVKSKRDYLRHRSSFSWLYLSRLAALKEFAFMKALEEHDFPVPKAIDCN 180

Query: 181 --------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKVTMIDFPQM 240
                   VKQLQNP+ +FE IIG+VVRLAEHGLIHCDFNEFNIMIDD+EK+TMIDFPQM
Sbjct: 181 RHCVIMVQVKQLQNPETIFEKIIGIVVRLAEHGLIHCDFNEFNIMIDDEEKITMIDFPQM 240

Query: 241 VSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPCFSSISKGA 300
           VSVSHRNAQMYFDRD+ECIFKFFRKRFNMSFHE   +  + E+  DE +RP F  I+K A
Sbjct: 241 VSVSHRNAQMYFDRDIECIFKFFRKRFNMSFHEDKGESEETEV--DENSRPSFFDITKDA 300

Query: 301 GSLDKELAASDCQRNE------------------DSDRESDGEGR--------------- 331
            +LDK+L AS   R E                  D D ESD E +               
Sbjct: 301 NALDKDLEASGFTRKEQTDLDKFIEGGVEKSEDSDEDEESDDEEQTCESNEEGNLNEIKS 360

[Close]

BLAST of Carg21615 vs. TAIR 10
Match: AT3G51270.1 (protein serine/threonine kinases;ATP binding;catalytics )

HSP 1 Score: 407.9 bits (1047), Expect = 7.8e-114
Identity = 248/464 (53.45%), Postives = 278/464 (59.91%), Query Frame = 0


Query: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVEN--------------------- 60
           MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVE                      
Sbjct: 1   MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIACLKHGGTYKVLKNLLKYKL 60

Query: 61  --------DGFRLTYLGYDFLAIKTLVNCGVFVAVGRQIGVGKESDIFEVAKEDGTVLAM 120
                   DGFRLTYLGYDFLAIKTLVN G+F  VGRQIGVGKESDIFEVA+EDGT+LAM
Sbjct: 61  LHHDSSKYDGFRLTYLGYDFLAIKTLVNRGIFTGVGRQIGVGKESDIFEVAQEDGTILAM 120

Query: 121 KLHRLGRISFRSVKSKRDYLRHRSSYNWLYLSRLAAFKEYAFMK---------------- 180
           KLHRLGR SFR+VKSKRDYLRHRSS++WLYLSRLAA KE+AFMK                
Sbjct: 121 KLHRLGRTSFRAVKSKRDYLRHRSSFSWLYLSRLAALKEFAFMKALEEHDFPVPKAIDCN 180

Query: 181 ----------------VKQLQNPDVVFETIIGLVVRLAEHGLIHCDFNEFNIMIDDDEKV 240
                           VKQLQNP+ +FE IIG+VVRLAEHGLIHCDFNEFNIMIDD+EK+
Sbjct: 181 RHCVIMSLVQGYPMVQVKQLQNPETIFEKIIGIVVRLAEHGLIHCDFNEFNIMIDDEEKI 240

Query: 241 TMIDFPQMVSVSHRNAQMYFDRDVECIFKFFRKRFNMSFHETVDDENDVEIDNDEGARPC 300
           TMIDFPQMVSVSHRNAQMYFDRD+ECIFKFFRKRFNMSFHE   +  + E+  DE +RP 
Sbjct: 241 TMIDFPQMVSVSHRNAQMYFDRDIECIFKFFRKRFNMSFHEDKGESEETEV--DENSRPS 300

Query: 301 FSSISKGAGSLDKELAASDCQRNE------------------DSDRESDGEGR------- 331
           F  I+K A +LDK+L AS   R E                  D D ESD E +       
Sbjct: 301 FFDITKDANALDKDLEASGFTRKEQTDLDKFIEGGVEKSEDSDEDEESDDEEQTCESNEE 360

The following BLAST results are available for this feature:

BLAST of Carg21615 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028358.1	2.5e-183	100.00	Serine/threonine-protein kinase rio2, partial [Cucurbita argyrosperma subsp. arg...	[more]
XP_023541015.1	1.7e-158	71.81	serine/threonine-protein kinase rio2-like [Cucurbita pepo subsp. pepo]	[more]
XP_023553068.1	9.7e-143	63.49	serine/threonine-protein kinase rio2-like isoform X2 [Cucurbita pepo subsp. pepo...	[more]
XP_023007804.1	9.7e-143	63.49	serine/threonine-protein kinase rio2 isoform X2 [Cucurbita maxima]	[more]
XP_022923288.1	9.7e-143	63.49	serine/threonine-protein kinase rio2 isoform X2 [Cucurbita moschata] >KAG6577587...	[more]

BLAST of Carg21615 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54T05	1.6e-71	44.20	Serine/threonine-protein kinase rio2 OS=Dictyostelium discoideum OX=44689 GN=rio...	[more]
Q9CQS5	4.2e-64	44.07	Serine/threonine-protein kinase RIO2 OS=Mus musculus OX=10090 GN=Riok2 PE=1 SV=1	[more]
Q9BVS4	2.3e-62	42.55	Serine/threonine-protein kinase RIO2 OS=Homo sapiens OX=9606 GN=RIOK2 PE=1 SV=2	[more]
Q9P7W5	8.5e-57	38.37	Serine/threonine-protein kinase rio2 OS=Schizosaccharomyces pombe (strain 972 / ...	[more]
Q95Q34	1.1e-53	36.89	Serine/threonine-protein kinase RIO2 OS=Caenorhabditis elegans OX=6239 GN=riok-2...	[more]

BLAST of Carg21615 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L5Z7	4.7e-143	63.49	Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1E945	4.7e-143	63.49	Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1L8N7	6.2e-143	63.35	Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1E5R3	6.2e-143	63.35	Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0L5Q5	4.1e-139	62.21	Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_3...	[more]

BLAST of Carg21615 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51270.2	3.5e-114	53.80	protein serine/threonine kinases;ATP binding;catalytics	[more]
AT3G51270.1	7.8e-114	53.45	protein serine/threonine kinases;ATP binding;catalytics	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000687	RIO kinase	SMART	SM00090	rio_2	coord: 35..229 e-value: 7.9E-26 score: 101.8
IPR015285	RIO2 kinase winged helix domain, N-terminal	PFAM	PF09202	Rio2_N	coord: 8..38 e-value: 1.6E-11 score: 44.2
None	No IPR available	PFAM	PF01163	RIO1	coord: 135..219 e-value: 1.1E-23 score: 83.8
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 132..278 e-value: 4.2E-39 score: 135.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 252..333
None	No IPR available	PANTHER	PTHR45852	SER/THR-PROTEIN KINASE RIO2	coord: 266..333
None	No IPR available	PANTHER	PTHR45852:SF2	BNAA01G19540D PROTEIN	coord: 135..274
None	No IPR available	PANTHER	PTHR45852:SF2	BNAA01G19540D PROTEIN	coord: 1..38
None	No IPR available	PANTHER	PTHR45852:SF2	BNAA01G19540D PROTEIN	coord: 266..333 coord: 39..135
None	No IPR available	PANTHER	PTHR45852	SER/THR-PROTEIN KINASE RIO2	coord: 1..38 coord: 39..135 coord: 135..274
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 1..40 e-value: 2.7E-14 score: 54.8
IPR030484	Serine/threonine-protein kinase Rio2	CDD	cd05144	RIO2_C	coord: 65..213 e-value: 8.80301E-90 score: 264.752
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 66..209
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 5..59

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21615-RA	Carg21615-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005622	intracellular anatomical structure
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity