Carg21377 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21377
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	AMP-dependent synthetase/ligase
Location	Carg_Chr01: 13096324 .. 13097246 (-)
RNA-Seq Expression	Carg21377
Synteny	Carg21377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTATGGAGAAGGAGACTCAACACATTGGCTTTGCTTCCTCCTTTCACCTTAACACTCCCATTATCTCTCTCAATCTCTCTCAATCTCCCTCCGCCGTCTGCAATTTCAATGGACGGAATGCACCGATGCTCTGCCAACTACTCTCCTCTCACTCCCATCACCTTCTTGGAGCGCTCCGCCGCCGTCTACGGTGAAAGACTTTCTCTCGTCCATGGAACTGTTCGCTACACTTGGACAGAGACGCTCCAGCGATGTACCAGGCTCGCTTCTGCCCTGGTTGACGTTGGGATCTCTCGCCGTGACGTGGTATTTATTCATTCTCAACTCTTCCATTCCCTTCATTTCTATGTATTTGTTTTCCTCTGCTCTTCGATTGTTAGTATCTGGATGTGGCGTGTTTACGTAGAATTCGAAATTATGAAATTGAAGTTATTGAAGCAGATTAACAGCATTAATGATCTAATTTTGTGGCGCAATACGATTCTGTCGATTAGGTTGCTGCTTTGGCACCCAACATTCCAGCGATGTACGAGCTTCACTTTGCTGTGCCAATGGCCGGTGCAGTTCTTTGCACCCTCAACATGCGCCACGATGCGGCAATGATTTGTACATTGCTGAGACATTCAGAAGCCAAAATCATTTTTGTAGACTACCAATTTCTACATATTGTAAAAGGAGCAATCGAAATTATGTCCAAGACGACGGAAGAGCTGCCTCGCGTTGTGATCATTAAAGAGTCTGATCAGCAATCCTCCTACACCAATGGATTCGACTCCAGTTCAGATGATTTGGAGTTCGAGAGGCTTTTAGGCACTGGAAAACTTGATTTCGAGATCAGACGTCCTCAAGATGAAGATGATCCAATTTCCCTGAACTATACTTCAGGCACAACATCAAGGCCAAAGGGTGTGATTTACTCCCAC

mRNA sequence

GTATGGAGAAGGAGACTCAACACATTGGCTTTGCTTCCTCCTTTCACCTTAACACTCCCATTATCTCTCTCAATCTCTCTCAATCTCCCTCCGCCGTCTGCAATTTCAATGGACGGAATGCACCGATGCTCTGCCAACTACTCTCCTCTCACTCCCATCACCTTCTTGGAGCGCTCCGCCGCCGTCTACGGTGAAAGACTTTCTCTCGTCCATGGAACTGTTCGCTACACTTGGACAGAGACGCTCCAGCGATGTACCAGGCTCGCTTCTGCCCTGGTTGACGTTGGGATCTCTCGCCGTGACGTGACGACGGAAGAGCTGCCTCGCGTTGTGATCATTAAAGAGTCTGATCAGCAATCCTCCTACACCAATGGATTCGACTCCAGTTCAGATGATTTGGAGTTCGAGAGGCTTTTAGGCACTGGAAAACTTGATTTCGAGATCAGACGTCCTCAAGATGAAGATGATCCAATTTCCCTGAACTATACTTCAGGCACAACATCAAGGCCAAAGGGTGTGATTTACTCCCAC

Coding sequence (CDS)

GTATGGAGAAGGAGACTCAACACATTGGCTTTGCTTCCTCCTTTCACCTTAACACTCCCATTATCTCTCTCAATCTCTCTCAATCTCCCTCCGCCGTCTGCAATTTCAATGGACGGAATGCACCGATGCTCTGCCAACTACTCTCCTCTCACTCCCATCACCTTCTTGGAGCGCTCCGCCGCCGTCTACGGTGAAAGACTTTCTCTCGTCCATGGAACTGTTCGCTACACTTGGACAGAGACGCTCCAGCGATGTACCAGGCTCGCTTCTGCCCTGGTTGACGTTGGGATCTCTCGCCGTGACGTGACGACGGAAGAGCTGCCTCGCGTTGTGATCATTAAAGAGTCTGATCAGCAATCCTCCTACACCAATGGATTCGACTCCAGTTCAGATGATTTGGAGTTCGAGAGGCTTTTAGGCACTGGAAAACTTGATTTCGAGATCAGACGTCCTCAAGATGAAGATGATCCAATTTCCCTGAACTATACTTCAGGCACAACATCAAGGCCAAAGGGTGTGATTTACTCCCAC

Protein sequence

VWRRRLNTLALLPPFTLTLPLSLSISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVGISRRDVTTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEIRRPQDEDDPISLNYTSGTTSRPKGVIYSH

Homology

[Close]

BLAST of Carg21377 vs. NCBI nr
Match: KAG7037945.1 (putative acyl-activating enzyme 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 350.5 bits (898), Expect = 8.4e-93
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   VWRRRLNTLALLPPFTLTLPLSLSISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSA 60
           VWRRRLNTLALLPPFTLTLPLSLSISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSA
Sbjct: 1   VWRRRLNTLALLPPFTLTLPLSLSISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSA 60

Query: 61  AVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVGISRRDVTTEELPRVVIIKESDQQS 120
           AVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVGISRRDVTTEELPRVVIIKESDQQS
Sbjct: 61  AVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVGISRRDVTTEELPRVVIIKESDQQS 120

Query: 121 SYTNGFDSSSDDLEFERLLGTGKLDFEIRRPQDEDDPISLNYTSGTTSRPKGVIYSH 178
           SYTNGFDSSSDDLEFERLLGTGKLDFEIRRPQDEDDPISLNYTSGTTSRPKGVIYSH
Sbjct: 121 SYTNGFDSSSDDLEFERLLGTGKLDFEIRRPQDEDDPISLNYTSGTTSRPKGVIYSH 177

[Close]

BLAST of Carg21377 vs. NCBI nr
Match: XP_023522426.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 295.0 bits (754), Expect = 4.2e-76
Identity = 167/245 (68.16%), Postives = 169/245 (68.98%), Query Frame = 0


Query: 1   VWRRRLNTLALLPPFTLTLPLSLSISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSA 60
           VWRRRLN LALL PFT TLPL LS S  LPP SAISMDGMHRCSANYSPLTPITFLERSA
Sbjct: 14  VWRRRLNKLALLLPFTFTLPLPLSTS--LPPSSAISMDGMHRCSANYSPLTPITFLERSA 73

Query: 61  AVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVGISRRDV------------------ 120
           AVYGERLSLVHGTVRYTWTETL+RCTRLASALVD+GISRRDV                  
Sbjct: 74  AVYGERLSLVHGTVRYTWTETLERCTRLASALVDIGISRRDVVAALAPNIPAMYELHFAV 133

Query: 121 --------------------------------------------------TTEELPRVVI 178
                                                             TTEELPRVVI
Sbjct: 134 PMAGAVLCTLNMRHDAAMICTLLRHSEAKIIFVDYQFLHIVKGAIEIMSKTTEELPRVVI 193

[Close]

BLAST of Carg21377 vs. NCBI nr
Match: XP_022940718.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 271.6 bits (693), Expect = 4.9e-69
Identity = 154/231 (66.67%), Postives = 156/231 (67.53%), Query Frame = 0


Query: 15  FTLTLPLSLSISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTV 74
           F L  P+   ISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTV
Sbjct: 14  FHLNTPI---ISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTV 73

Query: 75  RYTWTETLQRCTRLASALVDVGISRRDV-------------------------------- 134
           RYTWTETLQRCTRLASALVDVGIS RDV                                
Sbjct: 74  RYTWTETLQRCTRLASALVDVGISLRDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRH 133

Query: 135 ------------------------------------TTEELPRVVIIKESDQQSSYTNGF 178
                                               TTEELPRVVIIKESDQQSSYTNGF
Sbjct: 134 DAAMICTLLRHSEAKIIFVDYQFLHIVKGAIEIMSKTTEELPRVVIIKESDQQSSYTNGF 193

[Close]

BLAST of Carg21377 vs. NCBI nr
Match: KAG6608628.1 (O-fucosyltransferase 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 255.0 bits (650), Expect = 4.8e-64
Identity = 141/209 (67.46%), Postives = 141/209 (67.46%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG
Sbjct: 1   MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 60

Query: 97  ISRRDV------------------------------------------------------ 156
           ISRRDV                                                      
Sbjct: 61  ISRRDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMICTLLRHSEAKIIFVDYQ 120

Query: 157 --------------TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI 178
                         TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI
Sbjct: 121 FLHIVKGAIEIMSKTTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI 180

[Close]

BLAST of Carg21377 vs. NCBI nr
Match: XP_022982524.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 253.8 bits (647), Expect = 1.1e-63
Identity = 140/209 (66.99%), Postives = 141/209 (67.46%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETL+RCTRLASALVDVG
Sbjct: 1   MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLERCTRLASALVDVG 60

Query: 97  ISRRDV------------------------------------------------------ 156
           ISRRDV                                                      
Sbjct: 61  ISRRDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMICTLLRHSEAKIIFVDYQ 120

Query: 157 --------------TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI 178
                         TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI
Sbjct: 121 FLHIVKGAIEIMSKTTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI 180

[Close]

BLAST of Carg21377 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.2e-27
Identity = 84/223 (37.67%), Postives = 106/223 (47.53%), Query Frame = 0


Query: 31  PPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLAS 90
           P S  +++G+ R  AN+SPL+PITFLERSA VY +R SLV G+V++TW +T QRC RLAS
Sbjct: 42  PESWRTIEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLAS 101

Query: 91  ALVDVGISRRDVTTEELPRVVIIKE----------------------------------- 150
           AL ++GISR DV     P V  + E                                   
Sbjct: 102 ALTNLGISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKI 161

Query: 151 -----------------------------------------SDQQSSYTNGFDSSSDDLE 178
                                                    SD+ SS T      S D E
Sbjct: 162 LFVDHQLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFA-SKYSFDYE 221

[Close]

BLAST of Carg21377 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.2e-26
Identity = 74/211 (35.07%), Postives = 103/211 (48.82%), Query Frame = 0


Query: 35  ISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVD 94
           + M+G  +  ANY PLTPI+FL+RSA VY +R+S+V+G+V+YTW +T  RC R+ASAL  
Sbjct: 1   MKMEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQ 60

Query: 95  VGISRRDVTT-------------------------------------------------- 154
           +GIS  DV +                                                  
Sbjct: 61  LGISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFAD 120

Query: 155 ------------------EELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDF 178
                             +++P +V+I E   QS   +    S + +E+E ++  GK DF
Sbjct: 121 HQFLQIAEGACEILSNKGDKVPILVLIPEPLTQS--VSRKKRSEEMMEYEDVVAMGKSDF 180

[Close]

BLAST of Carg21377 vs. ExPASy Swiss-Prot
Match: Q9SFW5 (Probable acyl-activating enzyme 21 OS=Arabidopsis thaliana OX=3702 GN=AEE21 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.6e-25
Identity = 73/199 (36.68%), Postives = 94/199 (47.24%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           M+G  RCSANY PL+PI+FLER+A V+G R S+V+G ++YTW +T  RC RLASAL D+G
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 97  ISRRDVTTEELPRVVI-------------------------------------------- 156
           +SR DV     P V                                              
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 157 --------------IKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEIRRPQDEDDPI 178
                         I+E     + T      S   ++E  L TG  +F+  RP DE DPI
Sbjct: 121 FLSVAEESLSLLSNIEEKPLIITITENPTEQSKYEQYEDFLSTGNPNFKPIRPVDECDPI 180

[Close]

BLAST of Carg21377 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.6e-25
Identity = 82/225 (36.44%), Postives = 113/225 (50.22%), Query Frame = 0


Query: 17  LTLPLSLSISLNLPPPSAI--SMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTV 76
           L  P++L ++    PP+    SM+G+ +CSAN+ PL+PITFLERS+  Y +  SLV+G+V
Sbjct: 7   LHTPVALCVASPPAPPTTSWKSMEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSV 66

Query: 77  RYTWTETLQRCTRLASAL-VDVGISRRDVT---TEELPRV-------------------- 136
           RYTW +T  RC +LASAL   +GIS  DV    +  LP +                    
Sbjct: 67  RYTWAQTHHRCLKLASALTTHLGISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNAR 126

Query: 137 -------VIIKESDQQSSY-------------------------------TNGFDSSSDD 178
                   ++  S+ +  +                               +  F SSS D
Sbjct: 127 NDSAMVSTLLAHSEAKLIFVEPQLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYD 186

[Close]

BLAST of Carg21377 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.5e-25
Identity = 81/225 (36.00%), Postives = 112/225 (49.78%), Query Frame = 0


Query: 17  LTLPLSLSISLNLPPPSAI--SMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTV 76
           L  P++L ++    PP+    SM+G+ +CSAN+ PL+PITFLERS+  Y +  SLV+G+V
Sbjct: 7   LHTPVALCVASPPAPPTTSWKSMEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSV 66

Query: 77  RYTWTETLQRCTRLASAL-VDVGISRRDVT---TEELPRV-------------------- 136
           RYTW +T  RC +LASAL    GIS  DV    +  +P +                    
Sbjct: 67  RYTWAQTHHRCLKLASALTTHFGISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNAR 126

Query: 137 -------VIIKESDQQSSY-------------------------------TNGFDSSSDD 178
                   ++  S+ +  +                               +  F SSS D
Sbjct: 127 NDSAMVSTLLAHSEAKLIFVEPQLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYD 186

[Close]

BLAST of Carg21377 vs. ExPASy TrEMBL
Match: A0A6J1FQ31 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111446228 PE=4 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 2.4e-69
Identity = 154/231 (66.67%), Postives = 156/231 (67.53%), Query Frame = 0


Query: 15  FTLTLPLSLSISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTV 74
           F L  P+   ISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTV
Sbjct: 14  FHLNTPI---ISLNLPPPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTV 73

Query: 75  RYTWTETLQRCTRLASALVDVGISRRDV-------------------------------- 134
           RYTWTETLQRCTRLASALVDVGIS RDV                                
Sbjct: 74  RYTWTETLQRCTRLASALVDVGISLRDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRH 133

Query: 135 ------------------------------------TTEELPRVVIIKESDQQSSYTNGF 178
                                               TTEELPRVVIIKESDQQSSYTNGF
Sbjct: 134 DAAMICTLLRHSEAKIIFVDYQFLHIVKGAIEIMSKTTEELPRVVIIKESDQQSSYTNGF 193

[Close]

BLAST of Carg21377 vs. ExPASy TrEMBL
Match: A0A6J1J4T8 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111481315 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 5.2e-64
Identity = 140/209 (66.99%), Postives = 141/209 (67.46%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETL+RCTRLASALVDVG
Sbjct: 1   MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLERCTRLASALVDVG 60

Query: 97  ISRRDV------------------------------------------------------ 156
           ISRRDV                                                      
Sbjct: 61  ISRRDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMICTLLRHSEAKIIFVDYQ 120

Query: 157 --------------TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI 178
                         TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI
Sbjct: 121 FLHIVKGAIEIMSKTTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI 180

[Close]

BLAST of Carg21377 vs. ExPASy TrEMBL
Match: A0A6J1C834 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111009236 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 6.6e-43
Identity = 108/209 (51.67%), Postives = 121/209 (57.89%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           M+GM RCSAN   LTPITFLERSAAVYG+R SLV+G VRYTW +TLQRCTRLASALV  G
Sbjct: 1   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 60

Query: 97  ISRRDV------------------------------------------------------ 156
           ISR DV                                                      
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 120

Query: 157 --------------TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI 178
                         TTE+LP VV+I+E DQ SS  NG DS+ +DLEFE LL TG++DF+I
Sbjct: 121 FLPMVKRAIKLMSKTTEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 180

[Close]

BLAST of Carg21377 vs. ExPASy TrEMBL
Match: A0A0A0L3B4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658540 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.5e-42
Identity = 107/211 (50.71%), Postives = 121/211 (57.35%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           M+G+ RCSAN  PLTPITFLER+AAV+G+R+SLV+G VR+TW ETLQRCT+ ASALV VG
Sbjct: 1   MEGI-RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60

Query: 97  ISRRDV------------------------------------------------------ 156
           ISR DV                                                      
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120

Query: 157 ----------------TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDF 178
                             EELPR+VI++E D  SS  NGFDS+SDDLE+E LL TG LDF
Sbjct: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

[Close]

BLAST of Carg21377 vs. ExPASy TrEMBL
Match: A0A6J1BTA0 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111005519 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 5.6e-42
Identity = 105/209 (50.24%), Postives = 122/209 (58.37%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           MDG  RCSAN+ PLTPITFLERSAAVYG+R+SLV+G +RYTW +TLQRCTRLASALV +G
Sbjct: 1   MDGADRCSANHVPLTPITFLERSAAVYGDRISLVYGRLRYTWKDTLQRCTRLASALVRLG 60

Query: 97  ISRRDVTT---------------------------------------------------- 156
           I+  DV                                                      
Sbjct: 61  IAPGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNIRHDAAMVSTLLTHSEAKIIIVDYQ 120

Query: 157 ----------------EELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEI 178
                           E+LP VVII+E DQ SS+ +   S+S+DLEFE+LL TG+LDFEI
Sbjct: 121 FLHIVKGAIEVMSQRKEKLPLVVIIQEYDQPSSHKDRAGSASEDLEFEKLLATGELDFEI 180

[Close]

BLAST of Carg21377 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-28
Identity = 84/223 (37.67%), Postives = 106/223 (47.53%), Query Frame = 0


Query: 31  PPSAISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLAS 90
           P S  +++G+ R  AN+SPL+PITFLERSA VY +R SLV G+V++TW +T QRC RLAS
Sbjct: 42  PESWRTIEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLAS 101

Query: 91  ALVDVGISRRDVTTEELPRVVIIKE----------------------------------- 150
           AL ++GISR DV     P V  + E                                   
Sbjct: 102 ALTNLGISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKI 161

Query: 151 -----------------------------------------SDQQSSYTNGFDSSSDDLE 178
                                                    SD+ SS T      S D E
Sbjct: 162 LFVDHQLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFA-SKYSFDYE 221

[Close]

BLAST of Carg21377 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 118.2 bits (295), Expect = 6.5e-27
Identity = 74/211 (35.07%), Postives = 103/211 (48.82%), Query Frame = 0


Query: 35  ISMDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVD 94
           + M+G  +  ANY PLTPI+FL+RSA VY +R+S+V+G+V+YTW +T  RC R+ASAL  
Sbjct: 1   MKMEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQ 60

Query: 95  VGISRRDVTT-------------------------------------------------- 154
           +GIS  DV +                                                  
Sbjct: 61  LGISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFAD 120

Query: 155 ------------------EELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDF 178
                             +++P +V+I E   QS   +    S + +E+E ++  GK DF
Sbjct: 121 HQFLQIAEGACEILSNKGDKVPILVLIPEPLTQS--VSRKKRSEEMMEYEDVVAMGKSDF 180

[Close]

BLAST of Carg21377 vs. TAIR 10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.1e-26
Identity = 73/199 (36.68%), Postives = 94/199 (47.24%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           M+G  RCSANY PL+PI+FLER+A V+G R S+V+G ++YTW +T  RC RLASAL D+G
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 97  ISRRDVTTEELPRVVI-------------------------------------------- 156
           +SR DV     P V                                              
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 157 --------------IKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEIRRPQDEDDPI 178
                         I+E     + T      S   ++E  L TG  +F+  RP DE DPI
Sbjct: 121 FLSVAEESLSLLSNIEEKPLIITITENPTEQSKYEQYEDFLSTGNPNFKPIRPVDECDPI 180

[Close]

BLAST of Carg21377 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 100.1 bits (248), Expect = 1.8e-21
Identity = 66/207 (31.88%), Postives = 92/207 (44.44%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGT-VRYTWTETLQRCTRLASALVDV 96
           M+ M  C+AN  PLTPI FLER+A VYG+  S+V+G+   YTW ET  RC R+AS+L  +
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 97  GISRRDVT---------------------------------------------------- 156
           GI R DV                                                     
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 157 -------------TEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFEIRR 178
                        T + P +V I + +++    +  D +     ++ L+  G LDF+  R
Sbjct: 121 FSVDLAVEAISMMTTDPPILVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDFKWIR 180

[Close]

BLAST of Carg21377 vs. TAIR 10
Match: AT1G68270.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 98.2 bits (243), Expect = 7.0e-21
Identity = 66/208 (31.73%), Postives = 92/208 (44.23%), Query Frame = 0


Query: 37  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLQRCTRLASALVDVG 96
           MD M  C AN  PLTPITFL+R++  Y  R S+++G  R+TW +T  RC RLA++L+ + 
Sbjct: 1   MDNMELCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLISLN 60

Query: 97  ISRRDVTTEELPRVVIIKE----------------------------------------- 156
           I++ DV +   P    I E                                         
Sbjct: 61  IAKNDVVSVVAPNTPAIYEMHFAVPMAGAVLNPINTRLDATSITTILRHAQPKILFIHRN 120

Query: 157 --------------SDQQ--------SSYTNGFDSSSDDLEFERLLGTGK----LDFEIR 178
                          D Q          Y +    SS++L++E L+  G+    L   + 
Sbjct: 121 FEPLAREILHLLSCDDLQLNLLVIFIDEYNSAKRVSSEELDYESLIQMGEPTSPLVENMF 180

The following BLAST results are available for this feature:

BLAST of Carg21377 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037945.1	8.4e-93	100.00	putative acyl-activating enzyme 21, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_023522426.1	4.2e-76	68.16	probable acyl-activating enzyme 1, peroxisomal [Cucurbita pepo subsp. pepo]	[more]
XP_022940718.1	4.9e-69	66.67	probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata]	[more]
KAG6608628.1	4.8e-64	67.46	O-fucosyltransferase 16, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022982524.1	1.1e-63	66.99	probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima]	[more]

BLAST of Carg21377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEY5	2.2e-27	37.67	Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1	[more]
F4HUK6	9.2e-26	35.07	Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1	[more]
Q9SFW5	1.6e-25	36.68	Probable acyl-activating enzyme 21 OS=Arabidopsis thaliana OX=3702 GN=AEE21 PE=3...	[more]
M4IRL4	1.6e-25	36.44	Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1	[more]
M4IS92	3.5e-25	36.00	Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1	[more]

BLAST of Carg21377 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQ31	2.4e-69	66.67	probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1J4T8	5.2e-64	66.99	probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1C834	6.6e-43	51.67	probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN...	[more]
A0A0A0L3B4	1.5e-42	50.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658540 PE=4 SV=1	[more]
A0A6J1BTA0	5.6e-42	50.24	probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Carg21377 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G17650.1	1.6e-28	37.67	AMP-dependent synthetase and ligase family protein	[more]
AT1G20560.1	6.5e-27	35.07	acyl activating enzyme 1	[more]
AT1G76290.1	1.1e-26	36.68	AMP-dependent synthetase and ligase family protein	[more]
AT5G16370.1	1.8e-21	31.88	acyl activating enzyme 5	[more]
AT1G68270.1	7.0e-21	31.73	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.980		coord: 50..111 e-value: 3.5E-8 score: 35.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 157..177
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 155..177
None	No IPR available	PANTHER	PTHR43859	ACYL-ACTIVATING ENZYME	coord: 33..104 coord: 104..177
None	No IPR available	PANTHER	PTHR43859:SF5	ACYL-ACTIVATING ENZYME 2-RELATED	coord: 33..104 coord: 104..177
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 35..177
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 112..177 e-value: 7.0E-7 score: 30.3
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 123..177 e-value: 7.9E-9 score: 34.6
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 160..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21377-RA	Carg21377-RA	mRNA