Carg21348 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21348
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	short-chain dehydrogenase/reductase family 42E member 1-like
Location	Carg_Chr01: 13212374 .. 13214475 (+)
RNA-Seq Expression	Carg21348
Synteny	Carg21348

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATTTCACCGCCAACCAAGGCATCCAAGGCAACACCTTCGCCGTCACCGGCGCATTGGGCTTCGTCGGCTCTGCCCTCTGCTTAGACCTTGTCCGTAGAGGCGCTCTCCAGGTCCGAGCCTTTGACCTCCGTCCTGCCTCTCCTTGGTCCGATGACCTCAAGATTCACGGGGTCAACATCATCCAAGGTTCATCCCTATCTGCAATTCAGACCCCCTTTTACTGTTTATTTTGCGATTCACCTCGGCGCTTATATTCTCTCTGCCTTGATCGTCCTCTCGCTATTTGTCTCTGCTTTACTCTTTCTGGCTTTGATTCTATCTCTCTGTAGTGGAGTTAAATTGTGTGCCACAGTAGGCTTCTTTCGACGCTAACTCTTGCAGTCGTGTAGGGGATATTACCTGTAAAAAGGACGTGGAAAGAGCTCTTGGTGGTGTTGATTGTGTTTTCCACCTAGCAGCATATGGATTATCAGGGAAAGAAATGCTTCAAGTCGGTCGCATTGATGAGGTGAATATTAATGGAACATGCCATGTCTTGGATGCTTGCCTGGAGCACGGGGTTAGACGGCTTATATATATGAGCACGTATAATGTAGTGTTTGGAGGTCAAGAGATTGTTAACGGAAATGAGGGATTACCTTATTTCCCAATTGATGATCACGTTGATGCATATAGTCGAAGTAAATCCATTGCAGAACAATTAGTTCTGAAGACCAACGGGCGCCCCTTAAAGTAAGTCAAATCACTACTTTCTACTTCCGTGGTTTTCGCTTTTCTGGTTTGTTTACTTGTATTGGATTGCGCTTACTGCAACCATTAGTCTTTACTTTCTTGCGCAAGAGTGTATAATAACATTAGCTTCGCCTTTGGATTCTTAGGAACAGGGATGGCAAATGTTTTCACACCTGTGCTATACGTTCCTGTGCTATCTACGGACCTGGGGAAGAGAGGCATTTTCCTAGGATTGTCTCCTTAGCAAAGTTAGGCTTGCTACCATTCAGAGTTGGGAAACCAAGTGTTAAAACAGACTGGATCTATGTGGATAATCTCGTGCTTGCTTTAATATTGGCAAGCATGGGGCTTCTGGATGGTACTCCTGGTAAAGGAAGAGGCCCGATAGCTGCTGGCCAGCCGTATTATGTATCAGATGGTAACTCCACAGAATTTCTTACGTTTTACAACCCTCCCATCTACTCTTTGTTAGATTCCTTTAATGTCGTGCTGTGCTGTTTCTATTCTTATCAGGTTATCCAGTTAATTCCTTTGAATTCGTTCAACCACTGCTCAATAGCTTAGGTTATGAGTGGCCAAAGTTTTACATGCCTGTTTCTAAAGCTCTTCTCTTGGGGAAGTTTTTTGCTGCGTTGTACACAATATTATATCCTTGGTTGAATAGATGGTGGCTTCCACATCCCTTGATGCTTCCCGCTGAGATTTACAAGGTTTGTTTATTTTGTTTCATTCTTTTATGCTTGTGTCTGCTAACATGCAAATGGCCCTACTGGATGTTTGGACATCTGCTTGCAACCAAGAATTCTCTCCTTGACCATCCCAATAATGCTCCTTCGTAGTGTGAAGAATGATTCCATGCTGAGTTAATATAACAGTTCTTAATCAATTCCTCTGAATCCTAATTAGTTGTTTGCCTATATCGCCACAGGTTGGCATCAGCAATTATTTCTCGTATCTTAAAGCCAAGGAGGAGTTGGGCTATATTCCAATGGTGAGTTCGAAGGAGGGCATGGCCGCGACCATCTCATATTGGCAGGAGAGGAAGAGGAAGAGCCTCGATGGGCCAACAATATATGCGTGGTTGTTTTCTGTTGTTGGAATGAGCATGCTGTTTAGTGCAGCTTTCCTTCCAGACGTTGGACCTGTTCCATTCTTCAAAGCTATCAGCCTTTTCTTCTTTAGGTCGATTCAAGTGGTGAGGATGGTGTTTCTTGTAGCCCTTCTACTGCACGTTGGTGAGAGCATATATGCATGGTTCTTAGCAAGAAAGGTCGATCCTGCTAACTCCAGGGGATGGTTTTGGCAAACTTTTGCTCTAGGCTACTTTTCCTTGCGTTTCTTGTTGAGAAGAGCAAGGAGTCAGTAG

mRNA sequence

ATGCATTTCACCGCCAACCAAGGCATCCAAGGCAACACCTTCGCCGTCACCGGCGCATTGGGCTTCGTCGGCTCTGCCCTCTGCTTAGACCTTGTCCGTAGAGGCGCTCTCCAGGTCCGAGCCTTTGACCTCCGTCCTGCCTCTCCTTGGTCCGATGACCTCAAGATTCACGGGGCTTCTTTCGACGCTAACTCTTGCAGTCGTGTAGGGGATATTACCTGTAAAAAGGACGTGGAAAGAGCTCTTGGTGGTGTTGATTGTGTTTTCCACCTAGCAGCATATGGATTATCAGGGAAAGAAATGCTTCAAGTCGGTCGCATTGATGAGGTGAATATTAATGGAACATGCCATGTCTTGGATGCTTGCCTGGAGCACGGGGTTAGACGGCTTATATATATGAGCACGTATAATGTAGTGTTTGGAGGTCAAGAGATTGTTAACGGAAATGAGGGATTACCTTATTTCCCAATTGATGATCACGTTGATGCATATAGTCGAAGTAAATCCATTGCAGAACAATTAGTTCTGAAGACCAACGGGCGCCCCTTAAAGAACAGGGATGGCAAATGTTTTCACACCTGTGCTATACGTTCCTGTGCTATCTACGGACCTGGGGAAGAGAGGCATTTTCCTAGGATTGTCTCCTTAGCAAAGTTAGGCTTGCTACCATTCAGAGTTGGGAAACCAAGTGTTAAAACAGACTGGATCTATGTGGATAATCTCGTGCTTGCTTTAATATTGGCAAGCATGGGGCTTCTGGATGGTACTCCTGGTAAAGGAAGAGGCCCGATAGCTGCTGGCCAGCCGTATTATGTATCAGATGGTTATCCAGTTAATTCCTTTGAATTCGTTCAACCACTGCTCAATAGCTTAGGTTATGAGTGGCCAAAGTTTTACATGCCTGTTTCTAAAGCTCTTCTCTTGGGGAAGTTTTTTGCTGCGTTGTACACAATATTATATCCTTGGTTGAATAGATGGTGGCTTCCACATCCCTTGATGCTTCCCGCTGAGATTTACAAGGTTGGCATCAGCAATTATTTCTCGTATCTTAAAGCCAAGGAGGAGTTGGGCTATATTCCAATGGTGAGTTCGAAGGAGGGCATGGCCGCGACCATCTCATATTGGCAGGAGAGGAAGAGGAAGAGCCTCGATGGGCCAACAATATATGCGTGGTTGTTTTCTGTTGTTGGAATGAGCATGCTGTTTAGTGCAGCTTTCCTTCCAGACGTTGGACCTGTTCCATTCTTCAAAGCTATCAGCCTTTTCTTCTTTAGGTCGATTCAAGTGGTGAGGATGGTGTTTCTTGTAGCCCTTCTACTGCACGTTGGTGAGAGCATATATGCATGGTTCTTAGCAAGAAAGGTCGATCCTGCTAACTCCAGGGGATGGTTTTGGCAAACTTTTGCTCTAGGCTACTTTTCCTTGCGTTTCTTGTTGAGAAGAGCAAGGAGTCAGTAG

Coding sequence (CDS)

ATGCATTTCACCGCCAACCAAGGCATCCAAGGCAACACCTTCGCCGTCACCGGCGCATTGGGCTTCGTCGGCTCTGCCCTCTGCTTAGACCTTGTCCGTAGAGGCGCTCTCCAGGTCCGAGCCTTTGACCTCCGTCCTGCCTCTCCTTGGTCCGATGACCTCAAGATTCACGGGGCTTCTTTCGACGCTAACTCTTGCAGTCGTGTAGGGGATATTACCTGTAAAAAGGACGTGGAAAGAGCTCTTGGTGGTGTTGATTGTGTTTTCCACCTAGCAGCATATGGATTATCAGGGAAAGAAATGCTTCAAGTCGGTCGCATTGATGAGGTGAATATTAATGGAACATGCCATGTCTTGGATGCTTGCCTGGAGCACGGGGTTAGACGGCTTATATATATGAGCACGTATAATGTAGTGTTTGGAGGTCAAGAGATTGTTAACGGAAATGAGGGATTACCTTATTTCCCAATTGATGATCACGTTGATGCATATAGTCGAAGTAAATCCATTGCAGAACAATTAGTTCTGAAGACCAACGGGCGCCCCTTAAAGAACAGGGATGGCAAATGTTTTCACACCTGTGCTATACGTTCCTGTGCTATCTACGGACCTGGGGAAGAGAGGCATTTTCCTAGGATTGTCTCCTTAGCAAAGTTAGGCTTGCTACCATTCAGAGTTGGGAAACCAAGTGTTAAAACAGACTGGATCTATGTGGATAATCTCGTGCTTGCTTTAATATTGGCAAGCATGGGGCTTCTGGATGGTACTCCTGGTAAAGGAAGAGGCCCGATAGCTGCTGGCCAGCCGTATTATGTATCAGATGGTTATCCAGTTAATTCCTTTGAATTCGTTCAACCACTGCTCAATAGCTTAGGTTATGAGTGGCCAAAGTTTTACATGCCTGTTTCTAAAGCTCTTCTCTTGGGGAAGTTTTTTGCTGCGTTGTACACAATATTATATCCTTGGTTGAATAGATGGTGGCTTCCACATCCCTTGATGCTTCCCGCTGAGATTTACAAGGTTGGCATCAGCAATTATTTCTCGTATCTTAAAGCCAAGGAGGAGTTGGGCTATATTCCAATGGTGAGTTCGAAGGAGGGCATGGCCGCGACCATCTCATATTGGCAGGAGAGGAAGAGGAAGAGCCTCGATGGGCCAACAATATATGCGTGGTTGTTTTCTGTTGTTGGAATGAGCATGCTGTTTAGTGCAGCTTTCCTTCCAGACGTTGGACCTGTTCCATTCTTCAAAGCTATCAGCCTTTTCTTCTTTAGGTCGATTCAAGTGGTGAGGATGGTGTTTCTTGTAGCCCTTCTACTGCACGTTGGTGAGAGCATATATGCATGGTTCTTAGCAAGAAAGGTCGATCCTGCTAACTCCAGGGGATGGTTTTGGCAAACTTTTGCTCTAGGCTACTTTTCCTTGCGTTTCTTGTTGAGAAGAGCAAGGAGTCAGTAG

Protein sequence

MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGASFDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLDACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIPMVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAISLFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLRRARSQ

Homology

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BLAST of Carg21348 vs. NCBI nr
Match: KAG7037974.1 (Short-chain dehydrogenase/reductase family 42E member 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.5e-288
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS
Sbjct: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
           FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD
Sbjct: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG
Sbjct: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN
Sbjct: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM
Sbjct: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
           PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP
Sbjct: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
           MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS
Sbjct: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR
Sbjct: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480

Query: 481 RARSQ 486
           RARSQ
Sbjct: 481 RARSQ 485

[Close]

BLAST of Carg21348 vs. NCBI nr
Match: KAG6608656.1 (Short-chain dehydrogenase/reductase family 42E member 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 971.1 bits (2509), Expect = 3.6e-279
Identity = 473/485 (97.53%), Postives = 475/485 (97.94%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHG +
Sbjct: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGVN 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
                    GDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD
Sbjct: 61  IIQ------GDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG
Sbjct: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN
Sbjct: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM
Sbjct: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
           PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP
Sbjct: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
           MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS
Sbjct: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFA+GYFSLRFLLR
Sbjct: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFAIGYFSLRFLLR 479

Query: 481 RARSQ 486
           RARSQ
Sbjct: 481 RARSQ 479

[Close]

BLAST of Carg21348 vs. NCBI nr
Match: XP_022940596.1 (short-chain dehydrogenase/reductase family 42E member 1-like [Cucurbita moschata])

HSP 1 Score: 959.5 bits (2479), Expect = 1.1e-275
Identity = 471/488 (96.52%), Postives = 473/488 (96.93%), Query Frame = 0


Query: 1   MHFTAN---QGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIH 60
           MHF AN   QGIQGNTFAVTGALGFVGSALCL+LVRRGALQVRAFDLRPASPWSDDLKIH
Sbjct: 1   MHFPANQGIQGIQGNTFAVTGALGFVGSALCLELVRRGALQVRAFDLRPASPWSDDLKIH 60

Query: 61  GASFDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCH 120
           G +         GDITCKKDVERAL GVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCH
Sbjct: 61  GVNIIQ------GDITCKKDVERALRGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCH 120

Query: 121 VLDACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLK 180
           VLDACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLK
Sbjct: 121 VLDACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLK 180

Query: 181 TNGRPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIY 240
           TNGRPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIY
Sbjct: 181 TNGRPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIY 240

Query: 241 VDNLVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPK 300
           VDNLVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPK
Sbjct: 241 VDNLVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPK 300

Query: 301 FYMPVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELG 360
           FYMPVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELG
Sbjct: 301 FYMPVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELG 360

Query: 361 YIPMVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFK 420
           YIPMVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFK
Sbjct: 361 YIPMVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFK 420

Query: 421 AISLFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRF 480
           AISLFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRF
Sbjct: 421 AISLFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRF 480

Query: 481 LLRRARSQ 486
           LLRRARSQ
Sbjct: 481 LLRRARSQ 482

[Close]

BLAST of Carg21348 vs. NCBI nr
Match: XP_023524511.1 (short-chain dehydrogenase/reductase family 42E member 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 957.2 bits (2473), Expect = 5.4e-275
Identity = 466/485 (96.08%), Postives = 470/485 (96.91%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MHFTANQGI+GNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHG +
Sbjct: 1   MHFTANQGIEGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGVN 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
                    GDITCKKDVERAL GVDCVFHLAA+GLSGKEMLQVGR+DEVNINGTCHVLD
Sbjct: 61  IIQ------GDITCKKDVERALRGVDCVFHLAAFGLSGKEMLQVGRVDEVNINGTCHVLD 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG
Sbjct: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN
Sbjct: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLALILASMGLLD TPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM
Sbjct: 241 LVLALILASMGLLDDTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
           PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGY P
Sbjct: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYTP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
           MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS
Sbjct: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           L FFRSIQVVRMVFLVA LLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR
Sbjct: 421 LLFFRSIQVVRMVFLVAFLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 479

Query: 481 RARSQ 486
           RARSQ
Sbjct: 481 RARSQ 479

[Close]

BLAST of Carg21348 vs. NCBI nr
Match: XP_022981862.1 (short-chain dehydrogenase/reductase family 42E member 1 [Cucurbita maxima])

HSP 1 Score: 948.0 bits (2449), Expect = 3.3e-272
Identity = 464/485 (95.67%), Postives = 469/485 (96.70%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MHFTANQGIQGNTFAVTGALGFVGSALCL+LVRRGALQVRAFDLRPASPWSDDLKIHG +
Sbjct: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLELVRRGALQVRAFDLRPASPWSDDLKIHGVN 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
                    GDITCKKDVERAL GVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD
Sbjct: 61  IIQ------GDITCKKDVERALRGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAE+LVLKTNG
Sbjct: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAERLVLKTNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RPLK  DGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN
Sbjct: 181 RPLK--DGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLAL+LASMGLLD TPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM
Sbjct: 241 LVLALVLASMGLLDDTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
           PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFS+LKAKEELGYIP
Sbjct: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSHLKAKEELGYIP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
           MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS
Sbjct: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           L FFRSIQVVRMVFLVA LLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR
Sbjct: 421 LLFFRSIQVVRMVFLVAFLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 477

Query: 481 RARSQ 486
           RARSQ
Sbjct: 481 RARSQ 477

[Close]

BLAST of Carg21348 vs. ExPASy Swiss-Prot
Match: A8DZE7 (Short-chain dehydrogenase/reductase family 42E member 1 OS=Danio rerio OX=7955 GN=sdr42e1 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.3e-74
Identity = 161/367 (43.87%), Postives = 220/367 (59.95%), Query Frame = 0


Query: 12  NTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGASFDANSCSRVGD 71
           N+F +TG  G+ G  L   L++  + +V  FD+   SP   DL   G  F         D
Sbjct: 8   NSFLITGGGGYFGFRLACALLKTSS-KVVLFDV---SPPIQDLP-EGLIF------MRAD 67

Query: 72  ITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLDACLEHGVRRLI 131
           I     VE+A+ G  CVFH+A+YG+SG+E L    I+EVN+ GT ++L AC+ H V RLI
Sbjct: 68  IRDYAQVEKAVRGSHCVFHIASYGMSGREQLNRKLIEEVNVKGTENILRACVAHSVPRLI 127

Query: 132 YMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNRDGKCF 191
           Y ST+NVVFGGQEI NG+E LPY P+  H D YSR+KSIAE  VLK N   L N  G   
Sbjct: 128 YTSTFNVVFGGQEIKNGDESLPYLPLHLHPDHYSRTKSIAEMQVLKANNLALSNSTG-VL 187

Query: 192 HTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALILASMG 251
            TCA+R   IYGPGE+RH PRIVS  + G+  F  G P    ++++VDNLV A +LA+  
Sbjct: 188 RTCALRPAGIYGPGEQRHLPRIVSYIENGIFRFVYGDPDSLVEFVHVDNLVSAHLLAADA 247

Query: 252 LLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKALLLGKF 311
           L +    +     AAGQ Y++SDG PVN+FEF +PL+  LGY +P   +P+S       F
Sbjct: 248 LTEKQQCR-----AAGQAYFISDGRPVNNFEFFRPLVEGLGYSFPTLRLPISMIY----F 307

Query: 312 FAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIPMVSSKEGMAAT 371
           FA L  +++  + R +   PL+   E+YK G+++YFS  KA+EELGY P +   E +   
Sbjct: 308 FAFLTEMVHFVVGRIYNFQPLLTRTEVYKTGVTHYFSMRKAREELGYEPKLYDLEDV--- 350

Query: 372 ISYWQER 379
           + ++Q R
Sbjct: 368 VQWFQAR 350

[Close]

BLAST of Carg21348 vs. ExPASy Swiss-Prot
Match: Q32L94 (Short-chain dehydrogenase/reductase family 42E member 1 OS=Bos taurus OX=9913 GN=SDR42E1 PE=2 SV=2)

HSP 1 Score: 280.8 bits (717), Expect = 2.9e-74
Identity = 158/349 (45.27%), Postives = 210/349 (60.17%), Query Frame = 0


Query: 13  TFAVTGALGFVGSALCLDLVRRGALQVRAFDL-RPASPWSDDLKIHGASFDANSCSRVGD 72
           T  +TG  G+ G  L   L   G + V  FD+  PA    + ++             +GD
Sbjct: 10  TVLITGGGGYFGFRLGCALNLLG-VHVILFDISHPAQTIPEGIRF-----------ILGD 69

Query: 73  ITCKKDVERALGGVD--CVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLDACLEHGVRR 132
           I C  D+E A  GVD  CVFH+A+YG+SG+E L    I+E+N+ GT ++L AC   GV R
Sbjct: 70  IRCLSDIENAFQGVDVACVFHIASYGMSGREQLNRSLIEEINVGGTDNILQACRRRGVPR 129

Query: 133 LIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNRDGK 192
           L+Y ST+NV+FGGQ I NG+E LPY P+  H D YSR+KSIAE+ VL  NG  L+ R G 
Sbjct: 130 LVYTSTFNVIFGGQVIRNGDESLPYLPLHLHPDHYSRTKSIAEKKVLSANGTALE-RGGG 189

Query: 193 CFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALILAS 252
              TCA+R   IYGPGE+RH PRIVS  + GL  F  G P    ++++VDNLV A ILAS
Sbjct: 190 VLSTCALRPAGIYGPGEQRHLPRIVSYIEKGLFRFVYGDPKSLVEFVHVDNLVQAHILAS 249

Query: 253 MGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKALLLG 312
             L        +G IAAGQPY++SDG PVN+FEF +PL+  LGY++P   +P    L L 
Sbjct: 250 EAL-----KANKGHIAAGQPYFISDGRPVNNFEFFRPLVEGLGYKFPSTRLP----LTLI 309

Query: 313 KFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGY 359
             FA L  + +  L R +   P +   E+YK G+++YFS  KA++ELGY
Sbjct: 310 YCFAFLTEMTHFILGRLYNFQPFLTRTEVYKTGVTHYFSLEKARKELGY 336

[Close]

BLAST of Carg21348 vs. ExPASy Swiss-Prot
Match: Q4R7R1 (Short-chain dehydrogenase/reductase family 42E member 1 OS=Macaca fascicularis OX=9541 GN=SDR42E1 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.5e-73
Identity = 159/352 (45.17%), Postives = 210/352 (59.66%), Query Frame = 0


Query: 10  QGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDL-RPASPWSDDLKIHGASFDANSCSR 69
           Q  T  +TG  G+ G  L   L ++G + V  FD+  PA    + +K             
Sbjct: 7   QKETVLITGGGGYFGFRLGCALNQKG-VHVILFDISSPAETIPEGIKF-----------I 66

Query: 70  VGDITCKKDVERALGGVD--CVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLDACLEHG 129
            GDI    D+E+A    D  CVFH+A+YG+SG+E L    I+EVNI GT ++L AC    
Sbjct: 67  QGDICHLSDIEKAFQDADITCVFHIASYGMSGREQLNRNLIEEVNIGGTDNILQACQRRR 126

Query: 130 VRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNR 189
           V RL+Y ST+NV+FGGQ I NG+E LPY P+  H D YSR+KSIAE+ VL+ NG PL   
Sbjct: 127 VPRLVYTSTFNVIFGGQVIRNGDESLPYLPLHLHPDHYSRTKSIAEKKVLEANGTPLDRG 186

Query: 190 DGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALI 249
           DG    TCA+R   IYGPGE+RH PRIVS  + GL  F  G P    ++++VDNLV A I
Sbjct: 187 DG-VLRTCALRPAGIYGPGEQRHLPRIVSYIEKGLFKFVYGDPRSLVEFVHVDNLVQAHI 246

Query: 250 LASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKAL 309
           LAS  L        +G IA+GQPY++SDG PVN+FEF +PL+  LGY +P   +P    L
Sbjct: 247 LASEAL-----RADKGHIASGQPYFISDGRPVNNFEFFRPLVEGLGYTFPSTRLP----L 306

Query: 310 LLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGY 359
            L   FA L  +++  L R +   P +   E+YK G+++YFS  KAK+ELGY
Sbjct: 307 TLVYCFAFLTEMVHFILGRLYNFQPFLTRTEVYKTGVTHYFSLEKAKKELGY 336

[Close]

BLAST of Carg21348 vs. ExPASy Swiss-Prot
Match: Q9D665 (Short-chain dehydrogenase/reductase family 42E member 1 OS=Mus musculus OX=10090 GN=Sdr42e1 PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.2e-73
Identity = 157/352 (44.60%), Postives = 212/352 (60.23%), Query Frame = 0


Query: 13  TFAVTGALGFVGSALCLDLVRRGALQVRAFDL-RPASPWSDDLKIHGASFDANSCSRVGD 72
           T  +TG  G+ G  L   L ++GA +V  FD+ +PA    + +K              GD
Sbjct: 10  TVLITGGGGYFGFRLGCALNQKGA-RVILFDITQPAQNLPEGIKF-----------VCGD 69

Query: 73  ITCKKDVERALGG---VDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLDACLEHGVR 132
           I C  DVE A      V CVFH+A+YG+SG+E L   +I+EVN+ GT ++L ACLE GV 
Sbjct: 70  IRCLADVETAFQDAEKVACVFHVASYGMSGREQLNKTQIEEVNVGGTENILRACLERGVP 129

Query: 133 RLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNRDG 192
           RL+Y ST+NV+FGGQ I NG+E LPY P+  H D YSR+KSIAE+ VL+ NG   K  DG
Sbjct: 130 RLVYTSTFNVIFGGQVIRNGDESLPYLPLHLHPDHYSRTKSIAEKKVLEANGLAFKQGDG 189

Query: 193 KCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALILA 252
               TCAIR   IYG GE+RH PRIVS  + GL  F  G P    ++++VDNL  A ILA
Sbjct: 190 -ILRTCAIRPAGIYGAGEQRHLPRIVSYIERGLFRFVYGDPQSLVEFVHVDNLAKAHILA 249

Query: 253 SMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKALLL 312
           S  L        +G +A+GQPY++SDG PVN+FEF +PL+  LGY +P   +P++    L
Sbjct: 250 SEAL-----KADKGHVASGQPYFISDGRPVNNFEFFRPLVEGLGYTFPSTRLPLTLIYCL 309

Query: 313 GKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 361
               A L  + +  + R +   P +   E+YK G+++YFS  KAK+ELG+ P
Sbjct: 310 ----AFLVEMTHFIVGRLYNFQPFLTRTEVYKTGVTHYFSLEKAKKELGFEP 339

[Close]

BLAST of Carg21348 vs. ExPASy Swiss-Prot
Match: Q8WUS8 (Short-chain dehydrogenase/reductase family 42E member 1 OS=Homo sapiens OX=9606 GN=SDR42E1 PE=2 SV=2)

HSP 1 Score: 272.7 bits (696), Expect = 8.0e-72
Identity = 157/352 (44.60%), Postives = 206/352 (58.52%), Query Frame = 0


Query: 10  QGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDL-RPASPWSDDLKIHGASFDANSCSR 69
           Q  +  +TG  G+ G  L   L + G + V  FD+  PA    + +K             
Sbjct: 7   QKESVLITGGSGYFGFRLGCALNQNG-VHVILFDISSPAQTIPEGIKF-----------I 66

Query: 70  VGDITCKKDVERALGGVD--CVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLDACLEHG 129
            GDI    DVE+A    D  CVFH+A+YG+SG+E L    I EVN+ GT ++L  C    
Sbjct: 67  QGDIRHLSDVEKAFQDADVTCVFHIASYGMSGREQLNRNLIKEVNVRGTDNILQVCQRRR 126

Query: 130 VRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNR 189
           V RL+Y ST+NV+FGGQ I NG+E LPY P+  H D YSR+KSIAEQ VL+ N  PL   
Sbjct: 127 VPRLVYTSTFNVIFGGQVIRNGDESLPYLPLHLHPDHYSRTKSIAEQKVLEANATPLDRG 186

Query: 190 DGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALI 249
           DG    TCA+R   IYGPGE+RH PRIVS  + GL  F  G P    ++++VDNLV A I
Sbjct: 187 DG-VLRTCALRPAGIYGPGEQRHLPRIVSYIEKGLFKFVYGDPRSLVEFVHVDNLVQAHI 246

Query: 250 LASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKAL 309
           LAS  L        +G IA+GQPY++SDG PVN+FEF +PL+  LGY +P   +P    L
Sbjct: 247 LASEAL-----RADKGHIASGQPYFISDGRPVNNFEFFRPLVEGLGYTFPSTRLP----L 306

Query: 310 LLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGY 359
            L   FA L  +++  L R +   P +   E+YK G+++YFS  KAK+ELGY
Sbjct: 307 TLVYCFAFLTEMVHFILGRLYNFQPFLTRTEVYKTGVTHYFSLEKAKKELGY 336

[Close]

BLAST of Carg21348 vs. ExPASy TrEMBL
Match: A0A6J1FIX8 (short-chain dehydrogenase/reductase family 42E member 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446143 PE=4 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 5.2e-276
Identity = 471/488 (96.52%), Postives = 473/488 (96.93%), Query Frame = 0


Query: 1   MHFTAN---QGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIH 60
           MHF AN   QGIQGNTFAVTGALGFVGSALCL+LVRRGALQVRAFDLRPASPWSDDLKIH
Sbjct: 1   MHFPANQGIQGIQGNTFAVTGALGFVGSALCLELVRRGALQVRAFDLRPASPWSDDLKIH 60

Query: 61  GASFDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCH 120
           G +         GDITCKKDVERAL GVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCH
Sbjct: 61  GVNIIQ------GDITCKKDVERALRGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCH 120

Query: 121 VLDACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLK 180
           VLDACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLK
Sbjct: 121 VLDACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLK 180

Query: 181 TNGRPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIY 240
           TNGRPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIY
Sbjct: 181 TNGRPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIY 240

Query: 241 VDNLVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPK 300
           VDNLVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPK
Sbjct: 241 VDNLVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPK 300

Query: 301 FYMPVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELG 360
           FYMPVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELG
Sbjct: 301 FYMPVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELG 360

Query: 361 YIPMVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFK 420
           YIPMVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFK
Sbjct: 361 YIPMVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFK 420

Query: 421 AISLFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRF 480
           AISLFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRF
Sbjct: 421 AISLFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRF 480

Query: 481 LLRRARSQ 486
           LLRRARSQ
Sbjct: 481 LLRRARSQ 482

[Close]

BLAST of Carg21348 vs. ExPASy TrEMBL
Match: A0A6J1IV55 (short-chain dehydrogenase/reductase family 42E member 1 OS=Cucurbita maxima OX=3661 GN=LOC111480879 PE=4 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.6e-272
Identity = 464/485 (95.67%), Postives = 469/485 (96.70%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MHFTANQGIQGNTFAVTGALGFVGSALCL+LVRRGALQVRAFDLRPASPWSDDLKIHG +
Sbjct: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLELVRRGALQVRAFDLRPASPWSDDLKIHGVN 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
                    GDITCKKDVERAL GVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD
Sbjct: 61  IIQ------GDITCKKDVERALRGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAE+LVLKTNG
Sbjct: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAERLVLKTNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RPLK  DGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN
Sbjct: 181 RPLK--DGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLAL+LASMGLLD TPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM
Sbjct: 241 LVLALVLASMGLLDDTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
           PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFS+LKAKEELGYIP
Sbjct: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSHLKAKEELGYIP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
           MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS
Sbjct: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           L FFRSIQVVRMVFLVA LLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR
Sbjct: 421 LLFFRSIQVVRMVFLVAFLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 477

Query: 481 RARSQ 486
           RARSQ
Sbjct: 481 RARSQ 477

[Close]

BLAST of Carg21348 vs. ExPASy TrEMBL
Match: A0A6J1FA01 (short-chain dehydrogenase/reductase family 42E member 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443633 PE=4 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.6e-248
Identity = 415/484 (85.74%), Postives = 444/484 (91.74%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MH +AN+GI+ NTF VTGALGFVGSALCL+LVRRGALQVRAFDLRPASPWSDDLKIHG  
Sbjct: 1   MHLSANEGIEANTFVVTGALGFVGSALCLELVRRGALQVRAFDLRPASPWSDDLKIHGVK 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
                    GD+TCKKDVERA+ G DCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD
Sbjct: 61  IIQ------GDVTCKKDVERAVRGADCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           ACLE+GVRRL+YMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAY+RSKSIAEQLVLKTNG
Sbjct: 121 ACLEYGVRRLVYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYARSKSIAEQLVLKTNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RPLKNR+GKC +TCAIRSCAIYGPGEERHFPR+VSLAKLGLL FRVGKPS KTDWIYVDN
Sbjct: 181 RPLKNRNGKCLYTCAIRSCAIYGPGEERHFPRLVSLAKLGLLHFRVGKPSAKTDWIYVDN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLALILA +GLLD  P KG  P+AAGQPYYVSDG PVNS+EF++PLLNSLGY+ P  Y+
Sbjct: 241 LVLALILACVGLLDDIPVKGGAPVAAGQPYYVSDGCPVNSYEFMKPLLNSLGYDLPNSYL 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
           PV KALLLGKFFA LYTILYPWL+RWWLPHPLMLP EIYKVG+SNYFSYLKA+EELGY+P
Sbjct: 301 PVPKALLLGKFFAVLYTILYPWLDRWWLPHPLMLPPEIYKVGVSNYFSYLKAREELGYVP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
           MV+ KEGMAATISYWQERKRKSLDGPTIYAWLF VVGMS++F AAFLPDVGPVPFFK IS
Sbjct: 361 MVTPKEGMAATISYWQERKRKSLDGPTIYAWLFCVVGMSIMFCAAFLPDVGPVPFFKGIS 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           LFFFRSIQVVRMVFL+A LLHVGE  YAWFLARKVDPANSRGWFWQTFALG+FSLR LL+
Sbjct: 421 LFFFRSIQVVRMVFLIAFLLHVGEGTYAWFLARKVDPANSRGWFWQTFALGFFSLRLLLK 478

Query: 481 RARS 485
           RARS
Sbjct: 481 RARS 478

[Close]

BLAST of Carg21348 vs. ExPASy TrEMBL
Match: A0A6J1IIZ5 (short-chain dehydrogenase/reductase family 42E member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111474568 PE=4 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.7e-248
Identity = 414/484 (85.54%), Postives = 444/484 (91.74%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MH +AN+GI+ NTF VTGALGFVGSALCL+LVRRGALQVRAFDLRPASPWSDDLKIHG  
Sbjct: 1   MHLSANEGIEANTFVVTGALGFVGSALCLELVRRGALQVRAFDLRPASPWSDDLKIHGVK 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
                    GD+TCKKDVERA+ G DCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD
Sbjct: 61  IIQ------GDVTCKKDVERAVRGADCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           ACLE+GVRRL+YMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAY+RSKSIAEQLVLKTNG
Sbjct: 121 ACLEYGVRRLVYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYARSKSIAEQLVLKTNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RPLKNR+GKC +TCAIRSCAIYGPGEERHFPR+VSLAKLGLL FRVGKPS KTDWIYVDN
Sbjct: 181 RPLKNRNGKCLYTCAIRSCAIYGPGEERHFPRLVSLAKLGLLHFRVGKPSAKTDWIYVDN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLALILA +GLLD  P KG  P+AAGQPYYVSDG PVNS+EF++PLLNSLGY+ P  Y+
Sbjct: 241 LVLALILACVGLLDDIPVKGGAPVAAGQPYYVSDGCPVNSYEFIKPLLNSLGYDLPNSYL 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
           PV KALLLGKFFA LYTILYPWL+RWWLPHPLMLP EIYKVG+SNYFSYLKA+EELGY+P
Sbjct: 301 PVPKALLLGKFFAVLYTILYPWLDRWWLPHPLMLPPEIYKVGVSNYFSYLKAREELGYVP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
           MV+ KEGMAATISYWQ+RKRKSLDGPTIYAWLF VVGMS++F AAFLPDVGPVPFFK IS
Sbjct: 361 MVTPKEGMAATISYWQKRKRKSLDGPTIYAWLFCVVGMSIMFCAAFLPDVGPVPFFKGIS 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           LFFFRSIQVVRMVFL+A LLHVGE  YAWFLARKVDPANSRGWFWQTFALG+FSLR LL+
Sbjct: 421 LFFFRSIQVVRMVFLIAFLLHVGEGTYAWFLARKVDPANSRGWFWQTFALGFFSLRLLLK 478

Query: 481 RARS 485
           RARS
Sbjct: 481 RARS 478

[Close]

BLAST of Carg21348 vs. ExPASy TrEMBL
Match: A0A0A0L3F5 (3Beta_HSD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G664460 PE=4 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.8e-247
Identity = 415/484 (85.74%), Postives = 444/484 (91.74%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MH +A++GI+GNTF VTG LGF GSALCL+L+RRGALQVRAFDLRPASPWSD LK  G  
Sbjct: 1   MHLSASEGIEGNTFTVTGGLGFAGSALCLELLRRGALQVRAFDLRPASPWSDHLKTQGVK 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
                    GD+TCKKDVERAL GVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD
Sbjct: 61  IIQ------GDVTCKKDVERALRGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           ACLE GVRRLIYMSTYNVVFG QEIVNGNEGLPY PIDDHVDAY RSKSIAEQLVLKTNG
Sbjct: 121 ACLEFGVRRLIYMSTYNVVFGSQEIVNGNEGLPYLPIDDHVDAYGRSKSIAEQLVLKTNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RPLKNR+GKC HTCAIRSCAIYGPGEERHF R+VSLAKLGLLPFRVGK S KTDWIYVDN
Sbjct: 181 RPLKNRNGKCLHTCAIRSCAIYGPGEERHFTRLVSLAKLGLLPFRVGKQSAKTDWIYVDN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLALILASMGLLD  PGKG+ P+AAGQPYYVSDG+PVNSFEFV+PLLNSLGY+ P +Y+
Sbjct: 241 LVLALILASMGLLDDIPGKGKDPVAAGQPYYVSDGHPVNSFEFVKPLLNSLGYDLPNYYL 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
           PV KAL LGKFFA LYTILYPWL+RWWLPHPLMLPAEIYKVG+SNYFSYLKAKEELGY P
Sbjct: 301 PVPKALPLGKFFALLYTILYPWLDRWWLPHPLMLPAEIYKVGVSNYFSYLKAKEELGYAP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
           +V+ KEGMAATISYWQER+RKSLDGPTIY WLF +VGMS+LF AAFLPDVGPVPFFKAIS
Sbjct: 361 IVTPKEGMAATISYWQERERKSLDGPTIYVWLFCLVGMSILFCAAFLPDVGPVPFFKAIS 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           LFFFRSI+V+RMVFLVALLLHVGE+IYAWFLARKVDPANSRGWFWQTFALG+FSLRFLL+
Sbjct: 421 LFFFRSIKVLRMVFLVALLLHVGEAIYAWFLARKVDPANSRGWFWQTFALGFFSLRFLLK 478

Query: 481 RARS 485
           RAR+
Sbjct: 481 RARN 478

[Close]

BLAST of Carg21348 vs. TAIR 10
Match: AT2G33630.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 706.1 bits (1821), Expect = 2.0e-203
Identity = 331/483 (68.53%), Postives = 406/483 (84.06%), Query Frame = 0


Query: 1   MHFTANQGIQGNTFAVTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGAS 60
           MH + N+G++GNTF VTG LGFVG+ALCL+LVRRGA QVR+FDLR +SPWSDDLK  G  
Sbjct: 1   MHLSENEGVEGNTFVVTGGLGFVGAALCLELVRRGARQVRSFDLRHSSPWSDDLKNSGV- 60

Query: 61  FDANSCSRVGDITCKKDVERALGGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLD 120
                C + GD+T K+DV+ AL G DCV HLA+YG+SGKEML+ GR DEVNINGTC+VL+
Sbjct: 61  ----RCIQ-GDVTKKQDVDNALDGADCVLHLASYGMSGKEMLRFGRCDEVNINGTCNVLE 120

Query: 121 ACLEHGVRRLIYMSTYNVVFGGQEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNG 180
           A  +H + R++Y+STYNVVFGG+EI+NGNEGLPYFP+DDHVDAYSR+KSIAEQLVLK+NG
Sbjct: 121 AAFKHEITRIVYVSTYNVVFGGKEILNGNEGLPYFPLDDHVDAYSRTKSIAEQLVLKSNG 180

Query: 181 RPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDN 240
           RP KN  GK  +TCAIR  AIYGPGE+RH PRIV+L KLGL  F++G+PSVK+DWIYV+N
Sbjct: 181 RPFKN-GGKRMYTCAIRPAAIYGPGEDRHLPRIVTLTKLGLALFKIGEPSVKSDWIYVEN 240

Query: 241 LVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYM 300
           LVLA+ILASMGLLD  PG+   P+AAGQPY+VSDGYPVN+FEF++PLL SL Y+ PK  +
Sbjct: 241 LVLAIILASMGLLDDIPGREGQPVAAGQPYFVSDGYPVNTFEFLRPLLKSLDYDLPKCTI 300

Query: 301 PVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIP 360
            V  AL LGK F   YT+LYPWL++ WLP PL+LPAE+YKVG+++YFSYLKAKEELGY+P
Sbjct: 301 SVPFALSLGKIFQGFYTVLYPWLSKSWLPQPLVLPAEVYKVGVTHYFSYLKAKEELGYVP 360

Query: 361 MVSSKEGMAATISYWQERKRKSLDGPTIYAWLFSVVGMSMLFSAAFLPDVGPVPFFKAIS 420
             SSKEGMAATISYWQERKR+SLDGPT++ W+   +GMS LF+A +LPD+GPVPF +AI 
Sbjct: 361 FKSSKEGMAATISYWQERKRRSLDGPTMFTWIAVTIGMSALFAAGWLPDIGPVPFLRAIH 420

Query: 421 LFFFRSIQVVRMVFLVALLLHVGESIYAWFLARKVDPANSRGWFWQTFALGYFSLRFLLR 480
           LFFFR+I +V+ VF+VA++LHV E IYAWFLA++VDP N+ GWF QT ALG+FS+RFLL+
Sbjct: 421 LFFFRTITIVKAVFIVAVVLHVAEGIYAWFLAKRVDPGNAMGWFLQTSALGFFSMRFLLK 476

Query: 481 RAR 484
           RA+
Sbjct: 481 RAK 476

[Close]

BLAST of Carg21348 vs. TAIR 10
Match: AT1G47290.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 190.7 bits (483), Expect = 2.8e-48
Identity = 129/372 (34.68%), Postives = 190/372 (51.08%), Query Frame = 0


Query: 16  VTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWS--DDLKIHGASFDANSCSRV-GDI 75
           VTG  GF    L   LVR     VR  DL PA   +  ++  I G +  +     V  D+
Sbjct: 14  VTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYVSADL 73

Query: 76  TCKKDVERALGGVDCVFHLAAYGLS-GKEMLQVGRIDEVNINGTCHVLDACLEHGVRRLI 135
             K  V +   G + VFH+AA   S     LQ      VN+ GT +V+DAC+E GV+RLI
Sbjct: 74  RNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQY----SVNVQGTTNVIDACIEVGVKRLI 133

Query: 136 YMSTYNVVFGG-QEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNRDGKC 195
           Y S+ +VVF G    +N +E LPY P   H D+YS +K+  E L+LK NGR         
Sbjct: 134 YTSSPSVVFDGVHGTLNADESLPYPP--KHNDSYSATKAEGEALILKANGR-------SG 193

Query: 196 FHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALILASM 255
             TC IR  +I+GPG++   P +V+ A+ G   F +G  S   D+ YV+N+V A + A  
Sbjct: 194 LLTCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAER 253

Query: 256 GLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKALLLGK 315
            L  G     +   AAGQ Y++++  P+  +EF+  LL  LGYE P   +P S  + +  
Sbjct: 254 ALASGGEVCAK---AAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPI-- 313

Query: 316 FFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIPMVSSKEGMAA 375
             A L  + Y  L  + +  P++ P+ +  +  +  F   KAK+ LGY P+V  +EG+  
Sbjct: 314 --AYLVELAYKLLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKR 365

Query: 376 TISYWQERKRKS 383
           TI  +   K ++
Sbjct: 374 TIDSFSHLKAQN 365

[Close]

BLAST of Carg21348 vs. TAIR 10
Match: AT1G47290.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 190.7 bits (483), Expect = 2.8e-48
Identity = 129/372 (34.68%), Postives = 190/372 (51.08%), Query Frame = 0


Query: 16  VTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWS--DDLKIHGASFDANSCSRV-GDI 75
           VTG  GF    L   LVR     VR  DL PA   +  ++  I G +  +     V  D+
Sbjct: 14  VTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYVSADL 73

Query: 76  TCKKDVERALGGVDCVFHLAAYGLS-GKEMLQVGRIDEVNINGTCHVLDACLEHGVRRLI 135
             K  V +   G + VFH+AA   S     LQ      VN+ GT +V+DAC+E GV+RLI
Sbjct: 74  RNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQY----SVNVQGTTNVIDACIEVGVKRLI 133

Query: 136 YMSTYNVVFGG-QEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNRDGKC 195
           Y S+ +VVF G    +N +E LPY P   H D+YS +K+  E L+LK NGR         
Sbjct: 134 YTSSPSVVFDGVHGTLNADESLPYPP--KHNDSYSATKAEGEALILKANGR-------SG 193

Query: 196 FHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALILASM 255
             TC IR  +I+GPG++   P +V+ A+ G   F +G  S   D+ YV+N+V A + A  
Sbjct: 194 LLTCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAER 253

Query: 256 GLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKALLLGK 315
            L  G     +   AAGQ Y++++  P+  +EF+  LL  LGYE P   +P S  + +  
Sbjct: 254 ALASGGEVCAK---AAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPI-- 313

Query: 316 FFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIPMVSSKEGMAA 375
             A L  + Y  L  + +  P++ P+ +  +  +  F   KAK+ LGY P+V  +EG+  
Sbjct: 314 --AYLVELAYKLLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKR 365

Query: 376 TISYWQERKRKS 383
           TI  +   K ++
Sbjct: 374 TIDSFSHLKAQN 365

[Close]

BLAST of Carg21348 vs. TAIR 10
Match: AT2G26260.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 183.3 bits (464), Expect = 4.5e-46
Identity = 129/381 (33.86%), Postives = 191/381 (50.13%), Query Frame = 0


Query: 16  VTGALGFVGSALCLDLVRRGALQVRAFDLRPASPWSDDLKIHGASFDANSCSRV----GD 75
           VTG  GF    L   LVR     VR  DL PA    D  + +G   +     RV     D
Sbjct: 14  VTGGRGFAARHLVEMLVRYEMFCVRIADLAPAI-MLDPQEGNGVLDEGLRSGRVQYISAD 73

Query: 76  ITCKKDVERALGGVDCVFHLAAYGLS-GKEMLQVGRIDEVNINGTCHVLDACLEHGVRRL 135
           +  K  V +A  G + VFH+AA   S     LQ      VN+ GT +V+DAC++ GV+RL
Sbjct: 74  LRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQY----SVNVQGTQNVIDACVDVGVKRL 133

Query: 136 IYMSTYNVVFGG-QEIVNGNEGLPYFPIDDHVDAYSRSKSIAEQLVLKTNGRPLKNRDGK 195
           IY S+ +VVF G   I+NG E + Y PI  H D+YS +K+  E+L++K NGR        
Sbjct: 134 IYTSSPSVVFDGVHGILNGTESMAY-PI-KHNDSYSATKAEGEELIMKANGR-------N 193

Query: 196 CFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGKPSVKTDWIYVDNLVLALILAS 255
              TC IR  +I+GPG+    P +V+ A+ G   F +G  +   D+ YV+N+  A + A 
Sbjct: 194 GLLTCCIRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAE 253

Query: 256 MGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLLNSLGYEWPKFYMPVSKALLLG 315
             L  G     +   AAGQ Y++++  P+  +EF+  LL+ LGYE P   +P    + + 
Sbjct: 254 RALASGGDVSTK---AAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIA 313

Query: 316 KFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFSYLKAKEELGYIPMVSSKEGMA 375
                 Y +L P    + +  P + P+ +  +  S  F   KAK+ LGY P+V  +EG+ 
Sbjct: 314 HLVELTYKVLGP----YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIR 373

Query: 376 ATI---SYWQERKRKSLDGPT 388
            TI   S+     +   +GP+
Sbjct: 374 RTIDSFSHLTAGSQSKREGPS 373

[Close]

BLAST of Carg21348 vs. TAIR 10
Match: AT2G26260.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 159.5 bits (402), Expect = 7.0e-39
Identity = 97/282 (34.40%), Postives = 152/282 (53.90%), Query Frame = 0


Query: 110 VNINGTCHVLDACLEHGVRRLIYMSTYNVVFGG-QEIVNGNEGLPYFPIDDHVDAYSRSK 169
           VN+ GT +V+DAC++ GV+RLIY S+ +VVF G   I+NG E + Y PI  H D+YS +K
Sbjct: 17  VNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESMAY-PI-KHNDSYSATK 76

Query: 170 SIAEQLVLKTNGRPLKNRDGKCFHTCAIRSCAIYGPGEERHFPRIVSLAKLGLLPFRVGK 229
           +  E+L++K NGR           TC IR  +I+GPG+    P +V+ A+ G   F +G 
Sbjct: 77  AEGEELIMKANGR-------NGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGD 136

Query: 230 PSVKTDWIYVDNLVLALILASMGLLDGTPGKGRGPIAAGQPYYVSDGYPVNSFEFVQPLL 289
            +   D+ YV+N+  A + A   L  G     +   AAGQ Y++++  P+  +EF+  LL
Sbjct: 137 GNNLYDFTYVENVAHAHVCAERALASGGDVSTK---AAGQAYFITNMEPIKFWEFMSQLL 196

Query: 290 NSLGYEWPKFYMPVSKALLLGKFFAALYTILYPWLNRWWLPHPLMLPAEIYKVGISNYFS 349
           + LGYE P   +P    + +       Y +L P    + +  P + P+ +  +  S  F 
Sbjct: 197 DGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP----YGMTVPQLTPSRVRLLSCSRTFD 256

Query: 350 YLKAKEELGYIPMVSSKEGMAATI---SYWQERKRKSLDGPT 388
             KAK+ LGY P+V  +EG+  TI   S+     +   +GP+
Sbjct: 257 STKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSKREGPS 282

The following BLAST results are available for this feature:

BLAST of Carg21348 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037974.1	2.5e-288	100.00	Short-chain dehydrogenase/reductase family 42E member 1, partial [Cucurbita argy...	[more]
KAG6608656.1	3.6e-279	97.53	Short-chain dehydrogenase/reductase family 42E member 1, partial [Cucurbita argy...	[more]
XP_022940596.1	1.1e-275	96.52	short-chain dehydrogenase/reductase family 42E member 1-like [Cucurbita moschata...	[more]
XP_023524511.1	5.4e-275	96.08	short-chain dehydrogenase/reductase family 42E member 1 [Cucurbita pepo subsp. p...	[more]
XP_022981862.1	3.3e-272	95.67	short-chain dehydrogenase/reductase family 42E member 1 [Cucurbita maxima]	[more]

BLAST of Carg21348 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8DZE7	1.3e-74	43.87	Short-chain dehydrogenase/reductase family 42E member 1 OS=Danio rerio OX=7955 G...	[more]
Q32L94	2.9e-74	45.27	Short-chain dehydrogenase/reductase family 42E member 1 OS=Bos taurus OX=9913 GN...	[more]
Q4R7R1	2.5e-73	45.17	Short-chain dehydrogenase/reductase family 42E member 1 OS=Macaca fascicularis O...	[more]
Q9D665	3.2e-73	44.60	Short-chain dehydrogenase/reductase family 42E member 1 OS=Mus musculus OX=10090...	[more]
Q8WUS8	8.0e-72	44.60	Short-chain dehydrogenase/reductase family 42E member 1 OS=Homo sapiens OX=9606 ...	[more]

BLAST of Carg21348 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FIX8	5.2e-276	96.52	short-chain dehydrogenase/reductase family 42E member 1-like OS=Cucurbita moscha...	[more]
A0A6J1IV55	1.6e-272	95.67	short-chain dehydrogenase/reductase family 42E member 1 OS=Cucurbita maxima OX=3...	[more]
A0A6J1FA01	1.6e-248	85.74	short-chain dehydrogenase/reductase family 42E member 1-like OS=Cucurbita moscha...	[more]
A0A6J1IIZ5	2.7e-248	85.54	short-chain dehydrogenase/reductase family 42E member 1-like OS=Cucurbita maxima...	[more]
A0A0A0L3F5	1.8e-247	85.74	3Beta_HSD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G664460 P...	[more]

BLAST of Carg21348 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G33630.1	2.0e-203	68.53	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G47290.1	2.8e-48	34.68	3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1	[more]
AT1G47290.2	2.8e-48	34.68	3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1	[more]
AT2G26260.1	4.5e-46	33.86	3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2	[more]
AT2G26260.2	7.0e-39	34.40	3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR028110	Transmembrane protein 254	PFAM	PF14934	DUF4499	coord: 395..479 e-value: 4.0E-26 score: 91.1
None	No IPR available	GENE3D	3.40.50.720		coord: 3..381 e-value: 3.5E-59 score: 202.6
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 10..366
None	No IPR available	PANTHER	PTHR10366:SF317	SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 42E MEMBER 1	coord: 10..366
IPR002225	3-beta hydroxysteroid dehydrogenase/isomerase	PFAM	PF01073	3Beta_HSD	coord: 15..297 e-value: 5.7E-82 score: 274.9
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 13..378

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21348-RA	Carg21348-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006694	steroid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor