Carg21278 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21278
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
LocationCarg_Chr15: 9074619 .. 9077004 (+)
RNA-Seq ExpressionCarg21278
SyntenyCarg21278
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTGACAGATGAAGAGCGGCGTGGCCTGCAATTTCTGACACTTTTACCCTCCAATATGCTCCATTCTATCAAGTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCTCCGCCGAACACGTCACCGCCTCCGCCCTCCACCTCCCTTCCATCACCGCCATCATCACCGGTAACTCAACTCCAACCTCCTCTCTTACTCTCATCGTTAAACTCTACTTAATCTCTTCTTCTTTCAGGCGCGACGTCGGGTATTGGCGCGGAAACGGCGCGGGTTCTTGCCAAACGCGGGGCTCGGTTAATCCTCCCGTCGCGGAACCTGAAGGCGGCCGAGGAAGCCAAGGCGCGGATCGAAGCTGAATGCTCTGGCTCGGAAATCATCGCCATGGCTCTGGATTTAAGCTCTCTGGCCTCTGTTAGAACCTTCGTTGCCCAATTTGAATCGCTGAATCTGCCGCTGAATCTCCTCGTGTAAATCTCTGAAGAATCCATTAATTTCTGTTTTCCTCTGTTAATTGGGATTTTAATTTTTAAAAATTTTCAGAAACAACGCCGGGAAATTCTCTCACGAGCACGCGATTTCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTAATTAAAAAAAAATTAAAATTAAAATAGTTAAAATTAGAAATGGCGTATTAATGTTGTGAAGTGTTTTCAGGTCATTTTTTATTGACGAAATTGTTGGTGAATACGATGGTGGAGACGGCGAAGGCGACGGGGATTCAGGGGCGGATTGTGAACGTGTCGTCGAGCATTCACGGCTGGTTCTCCGGCGACATTATCAAATATCTCGGCCAGATAAGCCGAAATAAATGGTAAATTTAATATAAAAAAGGGTTTTCTTTTAATTTGTTTATTAATTTACAAATTTATCATGCACACAATTAAAATTTTCAAATTTTAAGACATAAAATTTAAGTTTATATATTATAAATTAATTAATATGAAATTTATTTCAAATTTAATATAATATAATTTTTAATTTTTTTTTAACATAATAATAATAATTTAGTATAAAAAGGGTTTTCTTTTTTAATTTTTCTACGTTTATTGACTTTAAAAATAAAAAATAAAATTTATGAATTCACAAATTTATGGACACAAAATAAAAAAAAAAATTAGGATATTAAATTCAAATTTATATATAACAAAGTAGTTAATATAAAAATTGTCTGTTTTAAAATTTATTAAAAATGAATAAAAATGGTATTTAATTTAATAGAATATATATTTTTATGGGTAAAAAAAGATGGTAATTCTAAAACAGTTACTGTGGGTGAATTTCCAGCAGGAATTACGATGCGACACGTGCGTATGCGCTCTCTAAGCTTGCAAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGGTTCTTTACATTTATAATATTACTTAAATACCCCACTCTTCCTTCTTTATTATAGGGTTTAATTCGTCTTTTCCTACTCACCTTTGACTCATATATATATATTTTTTAATTTGCATTAATCATTATTATTGATTCTTTTCTTATTAATTTTTTTAATGAAAAAGTAATTCTTAAAAAAAAAAAAGTTTTAGTTTAAAATTTATTATACAAAATTAAAAATTGTAATTTAAGAATTCTTGCATGAATTTGGTTAATTATTATTTTTTTATTATTTTAATTTAGAAAACGGGAGCTAATGTTACTGTGAATTGCGTTCATCCGGGAATCGTTAGAACTCGACTCACTAGAGACCGTGAAGGTTTCATTACAGGTACAAATTTTATTTAATTTTTCCTTTTATTATTTATTAAAATTAATTAATTTATTTTGCAGATTTGGTGTTCTTTATAACTTCTAAACTGTTGAAGACAATCCCTCAGGTAACCAACTTAAACCTCTTAATAATCTTGATTAAAAGGATAAGTTTTGCTTTATTGAATACATCTTCATTGGCATGAGGTCTTTTGGAGAAGTTTAAAGTAAAGTCACGAGAGTTTATACGTAAAGGGAACAATATCATACCATTGTAGAGAGTCGTAATTCCTAATAGTTCTGATATTGTTTTTCGTCTTCCACAGGCAGCGGCAACCACATGCTATGTGGCAGCAAACCCAAGACTGAGCAATGTCACCGGAAAATACTTTGCCGACTGCAACGAAGCCTCGCCGTCGAAACTCGCCGCTAGTCCGACGGAAGCCGCCCGTCTCTGGTCCGCGTCGGAGATCATGGTCAACACAAACTCAAAACTCGTTTTTAATCCAAACAATGCCTTGGACTAATTTAGTTTATTAAAAAAAAAAAAATAATAAATACCTCAAAAAATAAATATAAATATATATTGTTTGGTTAGGAGAGAGAATCTTAGT

mRNA sequence

AAATTGACAGATGAAGAGCGGCGTGGCCTGCAATTTCTGACACTTTTACCCTCCAATATGCTCCATTCTATCAAGTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCTCCGCCGAACACGTCACCGCCTCCGCCCTCCACCTCCCTTCCATCACCGCCATCATCACCGGTAACTCAACTCCAACCTCCTCTCTTACTCTCATCGTTAAACTCTACTTAATCTCTTCTTCTTTCAGGCGCGACGTCGGGTATTGGCGCGGAAACGGCGCGGGTTCTTGCCAAACGCGGGGCTCGGTTAATCCTCCCGTCGCGGAACCTGAAGGCGGCCGAGGAAGCCAAGGCGCGGATCGAAGCTGAATGCTCTGGCTCGGAAATCATCGCCATGGCTCTGGATTTAAGCTCTCTGGCCTCTGTTAGAACCTTCGTTGCCCAATTTGAATCGCTGAATCTGCCGCTGAATCTCCTCGTAAACAACGCCGGGAAATTCTCTCACGAGCACGCGATTTCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTCATTTTTTATTGACGAAATTGTTGGTGAATACGATGGTGGAGACGGCGAAGGCGACGGGGATTCAGGGGCGGATTGTGAACGTGTCGTCGAGCATTCACGGCTGGTTCTCCGGCGACATTATCAAATATCTCGGCCAGATAAGCCGAAATAAATGCAGGAATTACGATGCGACACGTGCGTATGCGCTCTCTAAGCTTGCAAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGAAAACGGGAGCTAATGTTACTGTGAATTGCGTTCATCCGGGAATCGTTAGAACTCGACTCACTAGAGACCGTGAAGGTTTCATTACAGATTTGGTGTTCTTTATAACTTCTAAACTGTTGAAGACAATCCCTCAGGCAGCGGCAACCACATGCTATGTGGCAGCAAACCCAAGACTGAGCAATGTCACCGGAAAATACTTTGCCGACTGCAACGAAGCCTCGCCGTCGAAACTCGCCGCTAGTCCGACGGAAGCCGCCCGTCTCTGGTCCGCGTCGGAGATCATGGTCAACACAAACTCAAAACTCGTTTTTAATCCAAACAATGCCTTGGACTAATTTAGTTTATTAAAAAAAAAAAAATAATAAATACCTCAAAAAATAAATATAAATATATATTGTTTGGTTAGGAGAGAGAATCTTAGT

Coding sequence (CDS)

ATGGCTCTGGATTTAAGCTCTCTGGCCTCTGTTAGAACCTTCGTTGCCCAATTTGAATCGCTGAATCTGCCGCTGAATCTCCTCGTAAACAACGCCGGGAAATTCTCTCACGAGCACGCGATTTCGGAGGACGGGATCGAGATGACCTTCGCCACTAATTATTTGGGTCATTTTTTATTGACGAAATTGTTGGTGAATACGATGGTGGAGACGGCGAAGGCGACGGGGATTCAGGGGCGGATTGTGAACGTGTCGTCGAGCATTCACGGCTGGTTCTCCGGCGACATTATCAAATATCTCGGCCAGATAAGCCGAAATAAATGCAGGAATTACGATGCGACACGTGCGTATGCGCTCTCTAAGCTTGCAAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGAAAACGGGAGCTAATGTTACTGTGAATTGCGTTCATCCGGGAATCGTTAGAACTCGACTCACTAGAGACCGTGAAGGTTTCATTACAGATTTGGTGTTCTTTATAACTTCTAAACTGTTGAAGACAATCCCTCAGGCAGCGGCAACCACATGCTATGTGGCAGCAAACCCAAGACTGAGCAATGTCACCGGAAAATACTTTGCCGACTGCAACGAAGCCTCGCCGTCGAAACTCGCCGCTAGTCCGACGGAAGCCGCCCGTCTCTGGTCCGCGTCGGAGATCATGGTCAACACAAACTCAAAACTCGTTTTTAATCCAAACAATGCCTTGGACTAA

Protein sequence

MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALSKLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIPQAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVFNPNNALD
Homology
BLAST of Carg21278 vs. NCBI nr
Match: KAG7017016.1 (Short-chain dehydrogenase TIC 32, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 483.4 bits (1243), Expect = 1.2e-132
Identity = 247/247 (100.00%), Postives = 247/247 (100.00%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS
Sbjct: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF
Sbjct: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240

Query: 241 NPNNALD 248
           NPNNALD
Sbjct: 241 NPNNALD 247

BLAST of Carg21278 vs. NCBI nr
Match: KAG6579556.1 (Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 483.4 bits (1243), Expect = 1.2e-132
Identity = 247/247 (100.00%), Postives = 247/247 (100.00%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 91  MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 150

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS
Sbjct: 151 TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 210

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 211 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 270

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF
Sbjct: 271 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 330

Query: 241 NPNNALD 248
           NPNNALD
Sbjct: 331 NPNNALD 337

BLAST of Carg21278 vs. NCBI nr
Match: XP_022929143.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 477.6 bits (1228), Expect = 6.3e-131
Identity = 244/247 (98.79%), Postives = 245/247 (99.19%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 91  MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 150

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVNTMVET KATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS
Sbjct: 151 TKLLVNTMVETTKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 210

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKKTGAN+TVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 211 KLANVLHTHELARRLKKTGANITVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 270

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAAS TEAARLWSASEIMVNTNSKLVF
Sbjct: 271 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASLTEAARLWSASEIMVNTNSKLVF 330

Query: 241 NPNNALD 248
           NPNNALD
Sbjct: 331 NPNNALD 337

BLAST of Carg21278 vs. NCBI nr
Match: XP_022970067.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 475.3 bits (1222), Expect = 3.1e-130
Identity = 243/247 (98.38%), Postives = 245/247 (99.19%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLL+NNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 91  MALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSHEHAISEDGIEMTFATNYLGHFLL 150

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS
Sbjct: 151 TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 210

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKK GANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 211 KLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 270

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAAS TEAARLWSASEIMVNTNSKLVF
Sbjct: 271 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASLTEAARLWSASEIMVNTNSKLVF 330

Query: 241 NPNNALD 248
           NPNNAL+
Sbjct: 331 NPNNALE 337

BLAST of Carg21278 vs. NCBI nr
Match: XP_023550055.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 471.1 bits (1211), Expect = 5.9e-129
Identity = 240/247 (97.17%), Postives = 243/247 (98.38%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLL+NNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 91  MALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSHEHAISEDGIEMTFATNYLGHFLL 150

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVN MVETA ATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS
Sbjct: 151 TKLLVNKMVETAAATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 210

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKK GANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 211 KLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 270

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSN+TGKYFADCNEASPSK+AAS TEAARLWSASEIMVNTNSKLVF
Sbjct: 271 QAAATTCYVAANPRLSNITGKYFADCNEASPSKIAASLTEAARLWSASEIMVNTNSKLVF 330

Query: 241 NPNNALD 248
           NPNNALD
Sbjct: 331 NPNNALD 337

BLAST of Carg21278 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-51
Identity = 113/236 (47.88%), Postives = 156/236 (66.10%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           + LDLSS+ SVR+F+ QF +LN+PLN+L+NNAG       +SEDGIE  FATN++GHFLL
Sbjct: 89  LQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFCPFQLSEDGIESQFATNHIGHFLL 148

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIH--GWFSGDIIKYLGQISRNKCRNYDATRAYA 120
           T LL++ M  +A+ +GI+GRIVN+SS  H   +  G +  Y+    R     Y   +AY 
Sbjct: 149 TNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGIMFDYINDPDR-----YSEKKAYG 208

Query: 121 LSKLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKT 180
            SKLAN+LH++ L+R+L++ G N+T+N VHPG++ T L R   G    ++  ++  L K 
Sbjct: 209 QSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-HSGLGMAVLKAMSFFLWKN 268

Query: 181 IPQAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNT 235
           IPQ AATTCYVA +P L +VTGKYFADCN  +PS  A   T A +LW  S  +V +
Sbjct: 269 IPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDTTLADKLWDFSIKLVES 318

BLAST of Carg21278 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 3.0e-51
Identity = 112/236 (47.46%), Postives = 155/236 (65.68%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           + LDLSS+ SVR+F+ QF +LN+PLN+L+NNAG       +SEDGIE  FATN++GHFLL
Sbjct: 89  LQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFCPFQLSEDGIESQFATNHIGHFLL 148

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIH--GWFSGDIIKYLGQISRNKCRNYDATRAYA 120
           T LL++ M  +A+ +GI+GRIVN+SS  H   +  G +  Y+    R     Y   +AY 
Sbjct: 149 TNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGIMFDYINDPDR-----YSEKKAYG 208

Query: 121 LSKLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKT 180
            SKLAN+LH++ L+R+L++ G N+T+N VHPG++ T L R   G    ++  ++  L K 
Sbjct: 209 QSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-HSGLGMAVLKAMSFFLWKN 268

Query: 181 IPQAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNT 235
           IPQ AATTCYVA +P L  VTGKYF DCN  +PS  A   T A +LW  S  +V++
Sbjct: 269 IPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDTTLADKLWDFSIKLVDS 318

BLAST of Carg21278 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 4.8e-49
Identity = 104/236 (44.07%), Postives = 152/236 (64.41%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           M LDLSS+ SVR F ++++S  LPLNLL+NNAG  +    +S+D IE+ FATN+LGHFLL
Sbjct: 85  MELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMACPFMLSKDNIELQFATNHLGHFLL 144

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLL++TM  T++ +  +GRIVN+SS  H +   + +++      N   +Y + RAY  S
Sbjct: 145 TKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGVRF---DKINDKSSYSSMRAYGQS 204

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KL NVLH +EL ++LK+ G N+T N +HPG + T L R    ++   V  +   +LK++P
Sbjct: 205 KLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRYFNPYLAVAVGAVAKYILKSVP 264

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNS 237
           Q AATTCYVA NP+++ V+G+YF D N A P  L      A ++W  S  + ++ S
Sbjct: 265 QGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTELAKKVWDFSTKLTDSQS 317

BLAST of Carg21278 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.0e-42
Identity = 97/226 (42.92%), Postives = 140/226 (61.95%), Query Frame = 0

Query: 3   LDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLLTK 62
           LDLSSL SV+ F ++F S   PLN+L+NNAG  +    +S+D IE+ FATN++GHFLLT 
Sbjct: 88  LDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLSKDNIELQFATNHIGHFLLTN 147

Query: 63  LLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALSKL 122
           LL++TM +T + +  +GRIVNV+S  H +   + I++      N   +Y+  RAY  SKL
Sbjct: 148 LLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF---DKINDQSSYNNWRAYGQSKL 207

Query: 123 ANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIPQA 182
           ANVLH ++L + LK+ G N+T N +HPG + T L R     +  L+  I   +LK + Q 
Sbjct: 208 ANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHNSA-VNGLINVIGKLVLKNVQQG 267

Query: 183 AATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSAS 229
           AATTCYVA +P++  V+G+YF+D N    +        A +LW  S
Sbjct: 268 AATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKKLWDFS 309

BLAST of Carg21278 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.4e-34
Identity = 96/232 (41.38%), Postives = 133/232 (57.33%), Query Frame = 0

Query: 3   LDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLLTK 62
           LDL+SL SVR F  +       L++L+NNAG F   +  +EDG EM F  N+LGHFLLT 
Sbjct: 114 LDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLGHFLLTN 173

Query: 63  LLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALSKL 122
           LL+  +  +A +     RIV VSS ++ +  GDI         N  ++Y+ +  Y+ SKL
Sbjct: 174 LLLGLLKSSAPS-----RIVVVSSKLYKY--GDI----NFDDLNSEQSYNKSFCYSRSKL 233

Query: 123 ANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDRE--GFITDLVFFITSKLLKTIP 182
           AN+L T ELARRL+  G NVTVN +HPGIVRT L R       +  L   ++    KT  
Sbjct: 234 ANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPLFNLVSWAFFKTPV 293

Query: 183 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMV 233
           + A T+ Y+A++P +  V+G+YF DC E      A   + A +LW  SE+MV
Sbjct: 294 EGAQTSIYLASSPEVEGVSGRYFGDCKEEELLPKAMDESVARKLWDISEVMV 332

BLAST of Carg21278 vs. ExPASy TrEMBL
Match: A0A6J1ER89 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435812 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.1e-131
Identity = 244/247 (98.79%), Postives = 245/247 (99.19%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 91  MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 150

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVNTMVET KATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS
Sbjct: 151 TKLLVNTMVETTKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 210

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKKTGAN+TVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 211 KLANVLHTHELARRLKKTGANITVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 270

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAAS TEAARLWSASEIMVNTNSKLVF
Sbjct: 271 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASLTEAARLWSASEIMVNTNSKLVF 330

Query: 241 NPNNALD 248
           NPNNALD
Sbjct: 331 NPNNALD 337

BLAST of Carg21278 vs. ExPASy TrEMBL
Match: A0A6J1HY34 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469067 PE=3 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 1.5e-130
Identity = 243/247 (98.38%), Postives = 245/247 (99.19%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLL+NNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 91  MALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSHEHAISEDGIEMTFATNYLGHFLL 150

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS
Sbjct: 151 TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 210

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKK GANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 211 KLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 270

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAAS TEAARLWSASEIMVNTNSKLVF
Sbjct: 271 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASLTEAARLWSASEIMVNTNSKLVF 330

Query: 241 NPNNALD 248
           NPNNAL+
Sbjct: 331 NPNNALE 337

BLAST of Carg21278 vs. ExPASy TrEMBL
Match: A0A6J1EMX3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435812 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 5.4e-128
Identity = 242/247 (97.98%), Postives = 243/247 (98.38%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 91  MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 150

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVNTMVET KATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNK  NYDATRAYALS
Sbjct: 151 TKLLVNTMVETTKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKW-NYDATRAYALS 210

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKKTGAN+TVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 211 KLANVLHTHELARRLKKTGANITVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 270

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAAS TEAARLWSASEIMVNTNSKLVF
Sbjct: 271 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASLTEAARLWSASEIMVNTNSKLVF 330

Query: 241 NPNNALD 248
           NPNNALD
Sbjct: 331 NPNNALD 336

BLAST of Carg21278 vs. ExPASy TrEMBL
Match: A0A6J1I2S6 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469067 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.7e-127
Identity = 241/247 (97.57%), Postives = 243/247 (98.38%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           MALDLSSLASVRTFVAQFESLNLPLNLL+NNAGKFSHEHAISEDGIEMTFATNYLGHFLL
Sbjct: 91  MALDLSSLASVRTFVAQFESLNLPLNLLINNAGKFSHEHAISEDGIEMTFATNYLGHFLL 150

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNK  NYDATRAYALS
Sbjct: 151 TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKW-NYDATRAYALS 210

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARRLKK GANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP
Sbjct: 211 KLANVLHTHELARRLKKMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 270

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAAS TEAARLWSASEIMVNTNSKLVF
Sbjct: 271 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASLTEAARLWSASEIMVNTNSKLVF 330

Query: 241 NPNNALD 248
           NPNNAL+
Sbjct: 331 NPNNALE 336

BLAST of Carg21278 vs. ExPASy TrEMBL
Match: A0A0A0KKE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149870 PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.4e-120
Identity = 220/247 (89.07%), Postives = 236/247 (95.55%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           M+LDLSSL+SVRTFV+QFESLNLPLNLL+NNAGKFSHEHAI+EDGIEMTFATNYLGHFLL
Sbjct: 89  MSLDLSSLSSVRTFVSQFESLNLPLNLLINNAGKFSHEHAITEDGIEMTFATNYLGHFLL 148

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLLVN MVETAK TG++GRIVNVSSSIHGWFSGDI+KYLGQISRNK RNYDATRAYALS
Sbjct: 149 TKLLVNKMVETAKVTGVEGRIVNVSSSIHGWFSGDILKYLGQISRNKSRNYDATRAYALS 208

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHTHELARR K+ GANVTVNCVHPGIVRTRLTRDREGFITDLVFF+ SKLLKTIP
Sbjct: 209 KLANVLHTHELARRFKQMGANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIP 268

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVA NPRL +VTGKYF DCNE+SPSKLA SP+EAARLWSASEIMVN NSKL+F
Sbjct: 269 QAAATTCYVATNPRLRHVTGKYFVDCNESSPSKLAGSPSEAARLWSASEIMVNANSKLLF 328

Query: 241 NPNNALD 248
           +PNNAL+
Sbjct: 329 DPNNALE 335

BLAST of Carg21278 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 367.9 bits (943), Expect = 6.6e-102
Identity = 181/242 (74.79%), Postives = 212/242 (87.60%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           M LDLSS+ASVR FVA FESL+LPLNLL+NNAGK +HEHAISEDGIEMTFATNYLGHFLL
Sbjct: 90  MKLDLSSIASVRNFVADFESLDLPLNLLINNAGKLAHEHAISEDGIEMTFATNYLGHFLL 149

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           T LL+N M++TA+ TG+QGRIVNV+S IHGWFSGD+I+YL  IS+ KC+ +DATRAYALS
Sbjct: 150 TNLLLNKMIQTAEETGVQGRIVNVTSGIHGWFSGDLIEYLRLISQPKCQ-FDATRAYALS 209

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHT EL+ RL+K GANVTVNCVHPG+VRTRLTRDREG +TDLVFF+ SKL+KT+P
Sbjct: 210 KLANVLHTKELSSRLQKIGANVTVNCVHPGVVRTRLTRDREGLLTDLVFFLASKLVKTVP 269

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSKLVF 240
           QAAATTCYVA NPRL NV+GKYF DCNE +PS L  + +EA +LW+ASEI+V  +SK  F
Sbjct: 270 QAAATTCYVATNPRLVNVSGKYFTDCNETTPSGLGTNSSEATKLWAASEILVTQHSKTSF 329

Query: 241 NP 243
           +P
Sbjct: 330 DP 330

BLAST of Carg21278 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 7.8e-95
Identity = 170/233 (72.96%), Postives = 200/233 (85.84%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           M LDLSSL SVR FV  FESLNLPLN+L+NNAGK++H+HA+SEDG+EMTFATNYLGHFLL
Sbjct: 90  MHLDLSSLTSVRRFVDDFESLNLPLNILINNAGKYAHKHALSEDGVEMTFATNYLGHFLL 149

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           TKLL+  M+ETA  TG+QGRIVNV+S +H WFSGD+++YL  ISRN  RNYDATRAYALS
Sbjct: 150 TKLLLKKMIETAAQTGVQGRIVNVTSVVHSWFSGDMLQYLADISRNN-RNYDATRAYALS 209

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLANVLHT EL+R L K  ANVT NCVHPGIV+TRLTRDREG +TDLVFF+TSKLLK++P
Sbjct: 210 KLANVLHTVELSRLLHKMDANVTANCVHPGIVKTRLTRDREGVVTDLVFFLTSKLLKSVP 269

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVN 234
           QAAATTCYVA +PRL NV GKYF+DCNEA  SK  +   +A RLW+AS+++V+
Sbjct: 270 QAAATTCYVATSPRLRNVCGKYFSDCNEARSSKSGSCNLKAQRLWTASDLLVS 321

BLAST of Carg21278 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 226.5 bits (576), Expect = 2.4e-59
Identity = 121/234 (51.71%), Postives = 161/234 (68.80%), Query Frame = 0

Query: 3   LDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLLTK 62
           +DLSSL+SV  F +QF S +LPLN+L+NNAG FS     SE+ IE+TFATN+LGH+LLT+
Sbjct: 94  IDLSSLSSVARFCSQFLSQDLPLNILINNAGVFSPNLEFSEEKIELTFATNFLGHYLLTE 153

Query: 63  LLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDII---KYLGQISRNKCRNYDATRAYAL 122
           +L+  M++TA+ +GI+GRI+N+SS IH W   D     K L  ISR     Y+ TRAYA 
Sbjct: 154 MLIEKMIDTAEKSGIEGRIINLSSVIHNWVKPDCFSFPKLLHPISR-----YNGTRAYAQ 213

Query: 123 SKLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTI 182
           SKLA +LH   L+++LK   ANVT+N VHPGIV+T + R  +G  TD +F I SKLLK+I
Sbjct: 214 SKLATILHAKALSKQLKDRNANVTINAVHPGIVKTGIIRAHKGLFTDSLFLIASKLLKSI 273

Query: 183 PQAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVN 234
            Q AATTCYVA +     ++GKYFADCNE + S LA     A +L + S  +++
Sbjct: 274 SQGAATTCYVALSNETKGLSGKYFADCNETNCSDLANDEYVALKLCTQSRALIH 322

BLAST of Carg21278 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 6.9e-59
Identity = 121/237 (51.05%), Postives = 164/237 (69.20%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           + LDLSS+ S++ FV +F +L+LPLNLL+NNAG     + +SEDGIE+ FATN++GHFLL
Sbjct: 89  LQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVMFCPYQLSEDGIELQFATNHIGHFLL 148

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           T LL++TM  TAK +G++GRI+NVSS  H +   + I++    S N   +Y   RAY  S
Sbjct: 149 TNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQEGIQF---DSINDICSYSDKRAYGQS 208

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KLAN+LH +EL+R+L++ G N+T N VHPG++ T L +     +  L FF +  L K IP
Sbjct: 209 KLANILHANELSRQLQEEGVNITANSVHPGLILTNLFQHTALLMRFLKFF-SFYLWKNIP 268

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSK 238
           Q AATTCYVA +P +  VTGKYFADCNE +PSKLA   T A +LW  S  ++N+ SK
Sbjct: 269 QGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETLAQKLWDFSVKLINSVSK 321

BLAST of Carg21278 vs. TAIR 10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 214.9 bits (546), Expect = 7.1e-56
Identity = 116/237 (48.95%), Postives = 159/237 (67.09%), Query Frame = 0

Query: 1   MALDLSSLASVRTFVAQFESLNLPLNLLVNNAGKFSHEHAISEDGIEMTFATNYLGHFLL 60
           + +D+SS+ SVR+FV QF +LN+PLN+L+NNAG       ++EDGIE  FATN++GHFLL
Sbjct: 89  LQIDVSSIKSVRSFVDQFLALNVPLNILINNAGVMFCPFKLTEDGIESQFATNHIGHFLL 148

Query: 61  TKLLVNTMVETAKATGIQGRIVNVSSSIHGWFSGDIIKYLGQISRNKCRNYDATRAYALS 120
           T LL++ M  TA+ +G+QGRIVN+SS  H +   + IK+ G    N    Y   RAY  S
Sbjct: 149 TNLLLDKMKSTARESGVQGRIVNLSSIAHTYTYSEGIKFQG---INDPAGYSERRAYGQS 208

Query: 121 KLANVLHTHELARRLKKTGANVTVNCVHPGIVRTRLTRDREGFITDLVFFITSKLLKTIP 180
           KL+N+LH++ L+RRL++ G N+T+N VHPG+V T L R   GF   +   +T    K IP
Sbjct: 209 KLSNLLHSNALSRRLQEEGVNITINSVHPGLVTTNLFR-YSGFSMKVFRAMTFLFWKNIP 268

Query: 181 QAAATTCYVAANPRLSNVTGKYFADCNEASPSKLAASPTEAARLWSASEIMVNTNSK 238
           Q AATTCYVA +P L  VTGKYF DCN  +PSK A + + A +LW  S  ++++ SK
Sbjct: 269 QGAATTCYVALHPDLEGVTGKYFGDCNIVAPSKFATNNSLADKLWDFSVFLIDSISK 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7017016.11.2e-132100.00Short-chain dehydrogenase TIC 32, chloroplastic [Cucurbita argyrosperma subsp. a... [more]
KAG6579556.11.2e-132100.00Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosper... [more]
XP_022929143.16.3e-13198.79short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita mosch... [more]
XP_022970067.13.1e-13098.38short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita maxim... [more]
XP_023550055.15.9e-12997.17short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
A0A078IS661.0e-5147.88Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078ISJ63.0e-5147.46Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A2RVM04.8e-4944.07Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q6RVV42.0e-4242.92Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
Q9HBH54.4e-3441.38Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ER893.1e-13198.79short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos... [more]
A0A6J1HY341.5e-13098.38short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max... [more]
A0A6J1EMX35.4e-12897.98short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos... [more]
A0A6J1I2S62.7e-12797.57short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max... [more]
A0A0A0KKE21.4e-12089.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149870 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G24050.16.6e-10274.79NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G64590.17.8e-9572.96NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.12.4e-5951.71NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02540.16.9e-5951.05NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G37540.17.1e-5648.95NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 117..136
score: 30.81
coord: 23..34
score: 45.43
coord: 140..157
score: 37.54
coord: 73..89
score: 29.47
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 117..136
score: 31.71
coord: 79..87
score: 54.94
coord: 23..34
score: 45.34
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 110..159
e-value: 4.6E-6
score: 26.2
coord: 2..87
e-value: 2.6E-12
score: 46.6
NoneNo IPR availableGENE3D3.40.50.720coord: 1..237
e-value: 2.4E-55
score: 189.6
NoneNo IPR availablePANTHERPTHR24320:SF210MITOCHONDRIAL PYRUVATE CARRIERcoord: 1..237
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 1..237
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 2..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21278-RACarg21278-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity