Carg21226 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21226
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	alpha-ketoglutarate-dependent dioxygenase alkB
Location	Carg_Chr18: 7710018 .. 7712610 (+)
RNA-Seq Expression	Carg21226
Synteny	Carg21226

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCCTAAGCTTCATTATTCCTGTCCCGTCCATCGCCATAGCTCAGAAGCTCAATTTCACGATCCTATCGAGTAACTCGCCGGCGGTAGCCGATCTCGGTGGATAAAATGTACGGTTCAGACAAAGGCACCGACGATTCTGAGCGCACCGCTTTCAGAAGAGCAGAGAAGAAATACAAACTGTACTACGACGACACCTATAAATCTTCCAAAAAGTATATTTCTTCCCAATTTGCGCTTTCAGGTTCTATTTCATTCAATTGGCATTGGATTCGTTGTTAGAGGCGATGTTTCATACTCTTGCTTATGATTCCTTTCAGGAAAAAGCTACCGAAACAAGTGGATTTGTCTGAGGTTATCGATTTCAAGCGTATCTTCGAATGTTACAATCAGGATGGTGCGCTTCCGCTGGGCGTGAATGCAACTAAGTGCGATCTCGATGGACCAGTCTTCTGCTTGGAGAATCGTCCTGGTATTGCTAATTCTCCATTTTCGATAGTGTTTCAAAGAAAAAGATCCCAAAATTCAGTTGTTCTCTATGTGTCTAAAATGTAGGGTTTTATTTTATTCCTGGTGCGTTGAGTTTAGAAGAACAATGCCAATGGATTAGGAAGAGTTTAAAGGATTTCCCGCAGCCTCCTAACAGAACCAATCATAATGCTATTTATGGACCAATTCAAGACCTGTTCATTGCTGCTAAGGAAAAGAGAGTTTTAGTTGAAGATAATGAGATCTCTGCTTCCAACGTTGATTCCGACACTGAACCTTCCGTCATCAATGGAAATTCTAATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGGAGGGGGACCTGCAAATCAGTTCCGGCCTCTGCATTACTTCGGAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGGTGAGTTTAACTAGAAGAATCCATATTTTACCCGTGTTTTATGTATGAACTCTTTGTAATATTAGGAGTGTTGAGAATTGTTAGGAGGGAGTCCCACGTTGGGCTAATTTAGGGGATGATTATGAGTTTATAAATAAGGAATACATCTCCATTGGTACGAGGCCTTTTGGGAAAACCAAAAGTAAAGTCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTTTGGAGGTTCGTGAATCCTAACATGGTATTAGAGTCATGCCCTTAATTTAGCAATGTCAATAGAATCCTCAAGTGTCGAACAAAGAAATTGCTAGTCTCGTAAGTGTAGTAAAAAATGACTCATGTGTCGAACAAAGGGTGTACTTTGTTTGAGGGTTTGAGAGAAAGGAGTCGAGCCTCGATTAAGGAGAGGCTGTCCAAGGGCTCTATAGACCTCAGGGGAGGCTCTATGGTGTACTATGTTCGAGGGGAGGATTGTTGGGAGGGAGTCCCACATTGGCTAATTTAGCGAATGATCATGGGTTTATAAGTAAGGAATACATCTCCATTGGTATGAGGCCTTTTGGGGAATCCCAAATCATAGCCATGAGAGTTCATGCTCCAAGTAGACAATATCATACCATTGTGGAAAGTCGTGATTCCTAACAAGAAGATTATACTGTATCTATGGATTTGTTTGATTTCTGAATTAATCTCCTTGTTATGGAGTAGCGAAGCTATGACATATCTCTGCCCCATAACGAGATTCCCTCTGCGTTATGTCAACTTACCAAAAGAATGGCGGCAGCTGCAATGCCTACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAACTATTTTGCTTCAGGTAAGTAAAGAAAAATTTGTTCATTGACATCAATAGAATGATAAGTAATGCATGGAAATGCCAGCAGAACCTTGCCATTACTCTGACACTTCGTTGATATCTTTAGGCGACACGCTCGGGGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGGTATCTTGCCAACTTATCCTCATTGAATTGCATTCCTGTCTGTTATGTTAAAGAGGACATTGATCTCCAGTACTTATTCTGTTATCTGCTTTATCCGCCCTCTCCAGTTTGGGTTGCAAGGCTATCTTTCTTTTGGGAGGCAAGTCGAGACAGGATCCGCCCGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGCAGCCCTCATGTTTACTCTTACATCATGCGTTGCCCTTTTGTTCTATATATCGACCCGGATATCCGATTTCAACGAATGCTCTGTCATTCATCTATCGAAAAAACCGCTAACTTCCTGAAACATGCCGTGAATGTCTTGTAGGTGTACCACGGATCTTCACAGATGAAGAAAGTGAGGAAATTTCTCTTCTTGAAAAGTGGTTTTCAAGTCGAGATGATTTGCATTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTCTGAGTGTTTCATGATTATTCTTTTACATTGCTAGAAGAAGTTTTCCATCATTACTGGGAGGCGATTGTTAGTTAGTTTTGGATGAAATTGGTTTGTACTTTGTTTAAAAAATACTCTTGAACTTTCAAAAG

mRNA sequence

AACCCTAAGCTTCATTATTCCTGTCCCGTCCATCGCCATAGCTCAGAAGCTCAATTTCACGATCCTATCGAGTAACTCGCCGGCGGTAGCCGATCTCGGTGGATAAAATGTACGGTTCAGACAAAGGCACCGACGATTCTGAGCGCACCGCTTTCAGAAGAGCAGAGAAGAAATACAAACTGTACTACGACGACACCTATAAATCTTCCAAAAAGAAAAAGCTACCGAAACAAGTGGATTTGTCTGAGGTTATCGATTTCAAGCGTATCTTCGAATGTTACAATCAGGATGGTGCGCTTCCGCTGGGCGTGAATGCAACTAAGTGCGATCTCGATGGACCAGTCTTCTGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGTGCGTTGAGTTTAGAAGAACAATGCCAATGGATTAGGAAGAGTTTAAAGGATTTCCCGCAGCCTCCTAACAGAACCAATCATAATGCTATTTATGGACCAATTCAAGACCTGTTCATTGCTGCTAAGGAAAAGAGAGTTTTAGTTGAAGATAATGAGATCTCTGCTTCCAACGTTGATTCCGACACTGAACCTTCCGTCATCAATGGAAATTCTAATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGGAGGGGGACCTGCAAATCAGTTCCGGCCTCTGCATTACTTCGGAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGGAGGGAGTCCCACGTTGGGCTAATTTAGGGGATGATTATGAGTTTATAAATAAGGAATACATCTCCATTGGTACGAGGCCTTTTGGGAAAACCAAAAGTAAAGTCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTTTGGAGCGAAGCTATGACATATCTCTGCCCCATAACGAGATTCCCTCTGCGTTATGTCAACTTACCAAAAGAATGGCGGCAGCTGCAATGCCTACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAACTATTTTGCTTCAGGCGACACGCTCGGGGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAGGCTATCTTTCTTTTGGGAGGCAAGTCGAGACAGGATCCGCCCGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTGTACCACGGATCTTCACAGATGAAGAAAGTGAGGAAATTTCTCTTCTTGAAAAGTGGTTTTCAAGTCGAGATGATTTGCATTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTCTGAGTGTTTCATGATTATTCTTTTACATTGCTAGAAGAAGTTTTCCATCATTACTGGGAGGCGATTGTTAGTTAGTTTTGGATGAAATTGGTTTGTACTTTGTTTAAAAAATACTCTTGAACTTTCAAAAG

Coding sequence (CDS)

ATGTACGGTTCAGACAAAGGCACCGACGATTCTGAGCGCACCGCTTTCAGAAGAGCAGAGAAGAAATACAAACTGTACTACGACGACACCTATAAATCTTCCAAAAAGAAAAAGCTACCGAAACAAGTGGATTTGTCTGAGGTTATCGATTTCAAGCGTATCTTCGAATGTTACAATCAGGATGGTGCGCTTCCGCTGGGCGTGAATGCAACTAAGTGCGATCTCGATGGACCAGTCTTCTGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGTGCGTTGAGTTTAGAAGAACAATGCCAATGGATTAGGAAGAGTTTAAAGGATTTCCCGCAGCCTCCTAACAGAACCAATCATAATGCTATTTATGGACCAATTCAAGACCTGTTCATTGCTGCTAAGGAAAAGAGAGTTTTAGTTGAAGATAATGAGATCTCTGCTTCCAACGTTGATTCCGACACTGAACCTTCCGTCATCAATGGAAATTCTAATGGATGGAAGTTTGTGGAGGAAAATACTGTTTCATCCAGGAGGGGGACCTGCAAATCAGTTCCGGCCTCTGCATTACTTCGGAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGGAGGGAGTCCCACGTTGGGCTAATTTAGGGGATGATTATGAGTTTATAAATAAGGAATACATCTCCATTGGTACGAGGCCTTTTGGGAAAACCAAAAGTAAAGTCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTTTGGAGCGAAGCTATGACATATCTCTGCCCCATAACGAGATTCCCTCTGCGTTATGTCAACTTACCAAAAGAATGGCGGCAGCTGCAATGCCTACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAACTATTTTGCTTCAGGCGACACGCTCGGGGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAGGCTATCTTTCTTTTGGGAGGCAAGTCGAGACAGGATCCGCCCGTAGCCATGTTTCTTCGAAGTGGAGATGTCGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTGTACCACGGATCTTCACAGATGAAGAAAGTGAGGAAATTTCTCTTCTTGAAAAGTGGTTTTCAAGTCGAGATGATTTGCATTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAGGTTTTCTGA

Protein sequence

MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQDGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNHNAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRGTCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKSKVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF

Homology

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BLAST of Carg21226 vs. NCBI nr
Match: KAG7012728.1 (Alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 849.7 bits (2194), Expect = 1.0e-242
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240
           TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240

Query: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
           KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY
Sbjct: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 412

[Close]

BLAST of Carg21226 vs. NCBI nr
Match: KAG6573589.1 (Alpha-ketoglutarate-dependent dioxygenase alkB, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 721.1 bits (1860), Expect = 5.5e-204
Identity = 362/412 (87.86%), Postives = 362/412 (87.86%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240
           TCKSVPASALLRKLRWSTLGLQFDWSK                                 
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSK--------------------------------- 240

Query: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
                            RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY
Sbjct: 241 -----------------RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 362

[Close]

BLAST of Carg21226 vs. NCBI nr
Match: XP_022945036.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata])

HSP 1 Score: 709.5 bits (1830), Expect = 1.7e-200
Identity = 357/412 (86.65%), Postives = 360/412 (87.38%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDD+ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPS+INGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240
           TCKSVPASALLRKLRWSTLGLQFDWSK                                 
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSK--------------------------------- 240

Query: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
                            RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY
Sbjct: 241 -----------------RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           ARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEYIRTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQVF 362

[Close]

BLAST of Carg21226 vs. NCBI nr
Match: XP_023541203.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701.4 bits (1809), Expect = 4.5e-198
Identity = 354/412 (85.92%), Postives = 358/412 (86.89%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFI GALSLEEQCQWIR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFISGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEK+VLVEDNEISASNVDSDTEPS+INGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240
           TCKSVPASALLRKLRWSTLGLQFDWSK                                 
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSK--------------------------------- 240

Query: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
                            RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY
Sbjct: 241 -----------------RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGDTLGGHLDDMEADWSKPIVS+SLGCKAIFLLGGKSRQD PVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDTLGGHLDDMEADWSKPIVSLSLGCKAIFLLGGKSRQDLPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           ARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEYIRTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQVF 362

[Close]

BLAST of Carg21226 vs. NCBI nr
Match: XP_022966806.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita maxima])

HSP 1 Score: 698.4 bits (1801), Expect = 3.8e-197
Identity = 354/412 (85.92%), Postives = 357/412 (86.65%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQ IR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQRIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240
           TCKSVPASALLRKLRWSTLGLQFDWSK                                 
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSK--------------------------------- 240

Query: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
                            RSYDISLPHN+IPSALCQL KRMAAAAMPTGEEFKPEAAIVNY
Sbjct: 241 -----------------RSYDISLPHNDIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQD PVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDLPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           ARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEY+RTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYMRTSRININIRQVF 362

[Close]

BLAST of Carg21226 vs. ExPASy Swiss-Prot
Match: Q9SA98 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 473.4 bits (1217), Expect = 2.6e-132
Identity = 245/413 (59.32%), Postives = 280/413 (67.80%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MY S   +DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I + +N 
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DG LP G+  +K D   PVFC++NRPGFYFIP ALSL+EQC+WI++SL  FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTV-SSRR 180
           NAIYGPI DLF +AKE +VLV+D+                   +N WKF EE  +  + R
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDL-----------------TNNKWKFYEEVDIEKATR 180

Query: 181 GTCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTK 240
            +CKSV AS LLRKLRWSTLGLQFDWSK                                
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSK-------------------------------- 240

Query: 241 SKVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVN 300
                             R+YD+SLPHN IP ALCQL K  AA AMP GEEF+PE AIVN
Sbjct: 241 ------------------RNYDVSLPHNNIPDALCQLAKTHAAIAMPDGEEFRPEGAIVN 300

Query: 301 YFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAG 360
           YF  GDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+LRSGDVVLMAG
Sbjct: 301 YFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYLRSGDVVLMAG 345

Query: 361 EARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           EARECFHG+PRIFT EE+ +I  LE   S      F EYI+TSRININIRQVF
Sbjct: 361 EARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIRQVF 345

[Close]

BLAST of Carg21226 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.8e-24
Identity = 74/221 (33.48%), Postives = 118/221 (53.39%), Query Frame = 0


Query: 213 WANLGDDYEFINKEYISIG----------TRPFGKTKSKV--------MRAYAQSGQYHT 272
           W N   + E IN+E  S G          TRP  K    +          A++  G  + 
Sbjct: 168 WKN--GEKELINEELKSQGKHDDDEIEQPTRPLDKNGEPLPTYRQLLDKLAWSTLGYQYQ 227

Query: 273 ILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDM 332
              R Y     + E P  L +L +++A A     + +  EAA VN+++    +GGHLDD 
Sbjct: 228 WTPRLYSEEF-YEEFPDDLQELVQKIAIAT--KFDPYVAEAATVNFYSEDSIMGGHLDDA 287

Query: 333 EADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFTD 392
           E +  KPI+S+S G  A+FL+G ++R   PV +F+RSGD+V+M G +R C+HGV +I   
Sbjct: 288 EQEMEKPIISISFGSTAVFLMGAETRDIAPVPLFIRSGDIVIMGGRSRYCYHGVAKIV-- 347

Query: 393 EESEEISLLEKWFSSRDDLHF-LEYI--RTSRININIRQVF 413
           E S ++ L+++  +   DL + ++++  +  R+NIN RQVF
Sbjct: 348 ENSFDLGLIDE--NDDQDLKYKIQWLKEKNRRVNINTRQVF 379

[Close]

BLAST of Carg21226 vs. ExPASy Swiss-Prot
Match: O60066 (Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=abh1 PE=2 SV=3)

HSP 1 Score: 110.2 bits (274), Expect = 5.8e-23
Identity = 103/403 (25.56%), Postives = 162/403 (40.20%), Query Frame = 0


Query: 12  ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQDGALPLGVNAT 71
           +   FR  EK+YK   D          +P   D+SEV+D          +     G  A 
Sbjct: 8   QANVFRLEEKRYKCRAD---------TIP---DMSEVLD---------PNDPQSFGFEAL 67

Query: 72  KCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSL-KDFPQPPNRTNHNAIYGPIQDL 131
             ++   VF  +  PG   +   +S E Q Q ++  +      P N+TN +  Y      
Sbjct: 68  -VEIKPRVFSFQKAPGLLILKNYVSSELQMQLLKSIMFTQIQDPENKTNLSPFY------ 127

Query: 132 FIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRGTCKSVPASAL 191
                  ++ + ++ I     + D E S+I+G                 G  K +    L
Sbjct: 128 -------QLPLGNDSIWRRYYNGDGE-SIIDG----------------LGETKPLTVDRL 187

Query: 192 L-RKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRP-FGKTKSKVMRAYAQ 251
           + +KLRW TLG Q+DW+                  KEY      P F K     +     
Sbjct: 188 VHKKLRWVTLGEQYDWT-----------------TKEYPDPSKSPGFPKDLGDFV----- 247

Query: 252 SGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNYFASGDTLG 311
                 +++ S D                             +K EAAIVN+++ GDTL 
Sbjct: 248 ----EKVVKESTDFL--------------------------HWKAEAAIVNFYSPGDTLS 298

Query: 312 GHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGV 371
            H+D+ E D + P++S+S+G   I+L+G +SR + P A+ L SGDVV+M G +R+ FH V
Sbjct: 308 AHIDESEEDLTLPLISLSMGLDCIYLIGTESRSEKPSALRLHSGDVVIMTGTSRKAFHAV 298

Query: 372 PRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQV 412
           P+I  +     +    K +          +I   R+N N+RQV
Sbjct: 368 PKIIPNSTPNYLLTGNKAWDG--------WISRKRVNFNVRQV 298

[Close]

BLAST of Carg21226 vs. ExPASy Swiss-Prot
Match: Q13686 (Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 1.3e-22
Identity = 75/238 (31.51%), Postives = 111/238 (46.64%), Query Frame = 0


Query: 189 ALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKSKVMRAYAQ 248
           +LL KLRW T+G  ++W                  +K+Y +    PF             
Sbjct: 163 SLLEKLRWVTVGYHYNWD-----------------SKKYSADHYTPF------------- 222

Query: 249 SGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNYFASGDTLG 308
                                PS L  L++++AAA     E+F+ EA I+NY+    TLG
Sbjct: 223 ---------------------PSDLGFLSEQVAAAC--GFEDFRAEAGILNYYRLDSTLG 282

Query: 309 GHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGV 368
            H+D  E D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H V
Sbjct: 283 IHVDRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAV 342

Query: 369 PRIFTDEESEEI---------------SLLEKWFSSRDDLHFLEYIRTSRININIRQV 412
           PR+  + E E +               S++E   S  D      Y++T+R+N+ +RQV
Sbjct: 343 PRVLPNPEGEGLPHCLEAPLPAVLPRDSMVEP-CSMEDWQVCASYLKTARVNMTVRQV 346

[Close]

BLAST of Carg21226 vs. ExPASy Swiss-Prot
Match: P0CB42 (Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.2e-21
Identity = 75/238 (31.51%), Postives = 108/238 (45.38%), Query Frame = 0


Query: 189 ALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKSKVMRAYAQ 248
           +LL +LRW TLG  ++W                  +K+Y +    PF             
Sbjct: 163 SLLERLRWVTLGYHYNWD-----------------SKKYSADHYTPF------------- 222

Query: 249 SGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNYFASGDTLG 308
                                PS L  L++++A A    G  F+ EA I+NY+    TLG
Sbjct: 223 ---------------------PSDLAFLSEQVATACGFQG--FQAEAGILNYYRLDSTLG 282

Query: 309 GHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGV 368
            H+D  E D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H V
Sbjct: 283 IHVDRSELDHSKPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNHAV 342

Query: 369 PRIFTDEESEEI---------------SLLEKWFSSRDDLHFLEYIRTSRININIRQV 412
           PR+    + E +               SL+E   S  D      Y+RT+R+N+ +RQV
Sbjct: 343 PRVLPHPDGECLPHCLETPLPAVLPSNSLVEP-CSVEDWQVCATYLRTARVNMTVRQV 346

[Close]

BLAST of Carg21226 vs. ExPASy TrEMBL
Match: A0A6J1FZQ9 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=LOC111449397 PE=4 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 8.0e-201
Identity = 357/412 (86.65%), Postives = 360/412 (87.38%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDD+ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPS+INGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240
           TCKSVPASALLRKLRWSTLGLQFDWSK                                 
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSK--------------------------------- 240

Query: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
                            RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY
Sbjct: 241 -----------------RSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           ARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEYIRTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIRQVF 362

[Close]

BLAST of Carg21226 vs. ExPASy TrEMBL
Match: A0A6J1HQB4 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LOC111466401 PE=4 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.8e-197
Identity = 354/412 (85.92%), Postives = 357/412 (86.65%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRI ECYNQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQ IR+SLKDFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQRIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240
           TCKSVPASALLRKLRWSTLGLQFDWSK                                 
Sbjct: 181 TCKSVPASALLRKLRWSTLGLQFDWSK--------------------------------- 240

Query: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
                            RSYDISLPHN+IPSALCQL KRMAAAAMPTGEEFKPEAAIVNY
Sbjct: 241 -----------------RSYDISLPHNDIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQD PVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDLPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           ARECFHGVPRIFTDEESEEISLLEK FSSRDDLHFLEY+RTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYMRTSRININIRQVF 362

[Close]

BLAST of Carg21226 vs. ExPASy TrEMBL
Match: A0A6J1DG90 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN=LOC111019811 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.4e-183
Identity = 328/412 (79.61%), Postives = 341/412 (82.77%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFKRI ECYN 
Sbjct: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DGALPLG+NAT+CDLDGPVFCLENRPGFYFIPGALSLEEQCQWIR+SL  FPQPPNRTNH
Sbjct: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEKRVLVE NEISA NVDSD EPSV  GN++GWKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRRT 180

Query: 181 TCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTKS 240
           TCKSVPAS LLRKLRWSTLGLQFDWSK                                 
Sbjct: 181 TCKSVPASVLLRKLRWSTLGLQFDWSK--------------------------------- 240

Query: 241 KVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVNY 300
                            RSYDISL HN++PSALCQL KRMAAAAMP GEEFKPEAAIVNY
Sbjct: 241 -----------------RSYDISLLHNKMPSALCQLAKRMAAAAMPAGEEFKPEAAIVNY 300

Query: 301 FASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360
           FASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE
Sbjct: 301 FASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGE 360

Query: 361 ARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           ARECFHGVPRIF D E++E SLLEK FS++DDLHFLEYIRTSRININIRQVF
Sbjct: 361 ARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIRQVF 362

[Close]

BLAST of Carg21226 vs. ExPASy TrEMBL
Match: A0A1S3BE32 (LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis melo OX=3656 GN=LOC103488884 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 3.0e-179
Identity = 321/413 (77.72%), Postives = 339/413 (82.08%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK I E Y Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DG+LP+GV AT CDLD PVFCLENRPGFYFIPGALSL+EQCQWIR+SL DFPQPPNRTNH
Sbjct: 61  DGSLPVGVKATTCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKEK+VLVE +EIS  N+DSD EPS+ NG+++ WKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180

Query: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTK 240
           T CKS+ AS LLRKLRWSTLGLQFDWSK                                
Sbjct: 181 TACKSISASVLLRKLRWSTLGLQFDWSK-------------------------------- 240

Query: 241 SKVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVN 300
                             RSY+ISLPHN+IPSALCQL KRMAAAAMPTGEEFKPEAAIVN
Sbjct: 241 ------------------RSYNISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVN 300

Query: 301 YFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAG 360
           YFASGDTLGGHLDDMEADWSKPIVSMSLGCKA FLLGGKSRQDPP+AMFLRSGDVVLMAG
Sbjct: 301 YFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGGKSRQDPPIAMFLRSGDVVLMAG 360

Query: 361 EARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           EARECFHGVPRIF DEESEEIS LE+  S++DDLHFLEYIRTSRININIRQVF
Sbjct: 361 EARECFHGVPRIFIDEESEEISFLERHLSNQDDLHFLEYIRTSRININIRQVF 363

[Close]

BLAST of Carg21226 vs. ExPASy TrEMBL
Match: A0A0A0KS00 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589370 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.5e-178
Identity = 320/413 (77.48%), Postives = 338/413 (81.84%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK I E Y Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DG+LP+GVNAT CDLDGPVFCLENRPGFYFIPGALSL+EQCQWIR+SL +FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180
           NAIYGPIQDLFIAAKE +VLVE +EIS   +DSD EPS+ NGN++ WKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 TC-KSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTK 240
           T  KS+PAS LLRKLRWSTLGLQFDWSK                                
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSK-------------------------------- 240

Query: 241 SKVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVN 300
                             RSY+ISLPHN+IPSALCQL KRMAAAAMPTGEEFKPEAAIVN
Sbjct: 241 ------------------RSYNISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVN 300

Query: 301 YFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAG 360
           YFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFLRSGDVVLMAG
Sbjct: 301 YFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFLRSGDVVLMAG 360

Query: 361 EARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           EARECFHGVPRIF DEESEEIS LE   +++DDLH LEYIRTSRININIRQVF
Sbjct: 361 EARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIRQVF 363

[Close]

BLAST of Carg21226 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 473.4 bits (1217), Expect = 1.9e-133
Identity = 245/413 (59.32%), Postives = 280/413 (67.80%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60
           MY S   +DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I + +N 
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120
           DG LP G+  +K D   PVFC++NRPGFYFIP ALSL+EQC+WI++SL  FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTV-SSRR 180
           NAIYGPI DLF +AKE +VLV+D+                   +N WKF EE  +  + R
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDL-----------------TNNKWKFYEEVDIEKATR 180

Query: 181 GTCKSVPASALLRKLRWSTLGLQFDWSKEGVPRWANLGDDYEFINKEYISIGTRPFGKTK 240
            +CKSV AS LLRKLRWSTLGLQFDWSK                                
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSK-------------------------------- 240

Query: 241 SKVMRAYAQSGQYHTILERSYDISLPHNEIPSALCQLTKRMAAAAMPTGEEFKPEAAIVN 300
                             R+YD+SLPHN IP ALCQL K  AA AMP GEEF+PE AIVN
Sbjct: 241 ------------------RNYDVSLPHNNIPDALCQLAKTHAAIAMPDGEEFRPEGAIVN 300

Query: 301 YFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAG 360
           YF  GDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+LRSGDVVLMAG
Sbjct: 301 YFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYLRSGDVVLMAG 345

Query: 361 EARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININIRQVF 413
           EARECFHG+PRIFT EE+ +I  LE   S      F EYI+TSRININIRQVF
Sbjct: 361 EARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIRQVF 345

[Close]

BLAST of Carg21226 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 5.2e-11
Identity = 42/122 (34.43%), Postives = 63/122 (51.64%), Query Frame = 0


Query: 293 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 352
           P+  IVN+++S   LG H D  E++ S     P+VS S+G  A FL G +  +D    + 
Sbjct: 346 PDICIVNFYSSTGRLGLHQDKDESENSIRKGLPVVSFSIGDSAEFLYGDQRDEDKAETLT 405

Query: 353 LRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININI 411
           L SGDV+L  G +R+ FHGV  I  D   +  +LL++             +R  R+N+  
Sbjct: 406 LESGDVLLFGGRSRKVFHGVRSIRKDTAPK--ALLQE-----------TSLRPGRLNLTF 454

[Close]

BLAST of Carg21226 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 5.2e-11
Identity = 43/121 (35.54%), Postives = 61/121 (50.41%), Query Frame = 0


Query: 293 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 352
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 278 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 337

Query: 353 LRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININI 410
           L SGDV++  GE+R  FHGV  I  +  S  +SLL +             +RT R+N+  
Sbjct: 338 LESGDVLIFGGESRMIFHGVKSIIPN--SAPMSLLNE-----------SKLRTGRLNLTF 385

[Close]

BLAST of Carg21226 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 5.2e-11
Identity = 43/121 (35.54%), Postives = 61/121 (50.41%), Query Frame = 0


Query: 293 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 352
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 333 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 392

Query: 353 LRSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKWFSSRDDLHFLEYIRTSRININI 410
           L SGDV++  GE+R  FHGV  I  +  S  +SLL +             +RT R+N+  
Sbjct: 393 LESGDVLIFGGESRMIFHGVKSIIPN--SAPMSLLNE-----------SKLRTGRLNLTF 440

[Close]

BLAST of Carg21226 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.1e-05
Identity = 32/104 (30.77%), Postives = 43/104 (41.35%), Query Frame = 0


Query: 293 PEAAIVNYFASGDTLGGH----LDDMEADWSK---------------------PIVSMSL 352
           P+  +VN++ S   LG H     D    D+ K                     PIVS S+
Sbjct: 351 PDICVVNFYTSTGKLGLHQVSVYDKTSFDFLKYKGGYLNTDKGESKKSLRKGLPIVSFSI 410

Query: 353 GCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRI 372
           G  A FL G +   D    + L SGDV++    +R  FHGV  I
Sbjct: 411 GDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHGVRSI 454

The following BLAST results are available for this feature:

BLAST of Carg21226 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012728.1	1.0e-242	100.00	Alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita argyrosperma subsp. ar...	[more]
KAG6573589.1	5.5e-204	87.86	Alpha-ketoglutarate-dependent dioxygenase alkB, partial [Cucurbita argyrosperma ...	[more]
XP_022945036.1	1.7e-200	86.65	alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata]	[more]
XP_023541203.1	4.5e-198	85.92	alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita pepo subsp. pepo]	[more]
XP_022966806.1	3.8e-197	85.92	alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita maxima]	[more]

BLAST of Carg21226 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SA98	2.6e-132	59.32	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 G...	[more]
Q54N08	1.8e-24	33.48	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44...	[more]
O60066	5.8e-23	25.56	Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (str...	[more]
Q13686	1.3e-22	31.51	Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2	[more]
P0CB42	3.2e-21	31.51	Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1	[more]

BLAST of Carg21226 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZQ9	8.0e-201	86.65	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1HQB4	1.8e-197	85.92	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1DG90	4.4e-183	79.61	alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN...	[more]
A0A1S3BE32	3.0e-179	77.72	LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis m...	[more]
A0A0A0KS00	1.5e-178	77.48	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]

BLAST of Carg21226 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11780.1	1.9e-133	59.32	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]
AT3G14160.1	5.2e-11	34.43	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.1	5.2e-11	35.54	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.2	5.2e-11	35.54	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT3G14140.1	1.1e-05	30.77	2-oxoglutarate-dependent dioxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 218..409 e-value: 2.5E-37 score: 129.0
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 165..411 e-value: 8.5E-43 score: 148.4
IPR004574	Alkylated DNA repair protein AlkB	PANTHER	PTHR16557	ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED	coord: 1..412
None	No IPR available	PANTHER	PTHR16557:SF8	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB	coord: 1..412
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 86..411
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 292..412 score: 9.285449

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21226-RA	Carg21226-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0035513	oxidative RNA demethylation
biological_process	GO:0035552	oxidative single-stranded DNA demethylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0035516	oxidative DNA demethylase activity
molecular_function	GO:0035515	oxidative RNA demethylase activity