Carg20990 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20990
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncalmodulin-binding receptor-like cytoplasmic kinase 2
LocationCarg_Chr14: 14384063 .. 14387424 (+)
RNA-Seq ExpressionCarg20990
SyntenyCarg20990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAATAGGTACCGCCATTTCTGAGAGTCTTGTTGGTTTTACTCTGTTAGCGCCAATGACGAGCTCAAGTCCGTGGCACGGCGGTGAGCGGCGGTATTCTAGCTCCAGCGGGGAGCGGCGGTATTCTAGTTCCAGCGGTGAGCGGCGGAATTCTAGCTCTGTCAGTGAGAGGCGGAACTCCAGCTCCCGTTGTAGTCGATCGCCTTACCTTTCTTACTTGCCCAGTCCCGATGTGTCCGCTGGTATGGAATCCCGTTCCCGACTTAATCCGGTGAAAGCCGCTGCACGGTCCGTCGCCGGAGCTTTCATTACTTGCTTTACACCGCCGGAGAAGGAAACTTCGCCGGGTTTTGGTATTGGTAGCGAATTTCGTCATTCTTCAGGTAATACTTGATTTTAAGCTTGTGGAATGAGAACGACTTGGATCACCTGTCTTAGCTTTTGGTTTATCTTTTAAGAAACTGGTAGGGAAGATGAATGAATAGGTCTCGTTGCGGCGTCTTTTCAGAGCTGTTAACCTACTGTTTAATGTTATCTCTTTTCTGTTGGTTTTTGGATCGTGGATGTTCGTCTGAAATTTTGGTGAAGATTTTAGTCTAATTGCTTGTTTTCTACACTATCTCAACTTGGAAATTCCCTGTTCTTGATAATTGCAAAAACATATTCTCCAAGACTCGCTTATCTGTTTCTAATCGTAGATTGTCTGAGATAGAGGCTTTCTTAACCTTGTGATACATTGAGATTTGAAACTCTTTGTCTTTTGTGCATTAAATTCTATCATTTATTTGTTAGATAACTAGAGGAAGATAGGAACAAGTGGATTTTCCGAATCATTTATGAAGTTCCTGAAATTGAGTCAGTTAAGAAACTGCAATTACCAATTTCAGTCTTCGTGGGCTGTCTGGATTCAAATTGTTCTCTCTTTCAAAGGGAAAATAAGCAGTAAACCACTTATTCCGATTGTGATCTTAGGATGAATTTGTGTGTTTTATATCTCTGTAATTCAGATGCTTCCAGTCATAATAGCAAAAGAAATCAAGGATCTAGGCGAGAAACCCACTTCAGTTCAAATCTAGCTGCCCATGATCGAGAGCCTGGATCTTTAAGGATCAGCATGGAAGAAATCCGCAAGGCTACGAAGAACTTCTCCGCATCTTCAAAAATTGGCCAAGGGGGTTTTGGGGCTGTCTACAAGGGGAAACTTGATGGAGTTCTTGTGGCAATAAAACGTGCCAAGAAGGTTAAATTTGATTCTTAAGCAGTTTATGGCTTATTGCTTTCAATCACCATCTCTGTTCATTGTTGTTAACGTGGATTCAATTTTTTTTTTTTCTTTCATAACCTCTTTCTCTGCAGAGTGTATATGATAATAATTTGGGCGTGGAGTTTCAAAGCGAGATCCAAACACTTGCACAAGTAGAACATTTAAATCTGGTTAAGTTCTATGGATATCTGGAACATGAAGATGAAAGAATCGTCATTGTTGAGTATGTGCCGAATGGGACACTCAGAGAGCACTTAGAATGTGAGTTACTTTGTTTCAGCTTTATCCTCTCTTTAAACTACACAGTTTAGTACTCTTATCTGAATCACCAAACTTGATGGCAGGCATCCATGGCACTACTCTTGACCTTGCTACGAGGCTCGCCATTGCAACCGATGTGGCTCATGCAATAACGTATCTTCATATGTATACAGGTATGGATTTGATTTGGTGCTCAACTCATCTTAACTATATAGAACCTAGTGCGCGTCTGGAATGACTTTTGGGAGGCTGAAAAGTGTTTCTATGGGCCAATGGCACTTTTAAAACAAAACATGTACAGTTCTTTAAGGAAAGATGCTCAATTAATGATTCTTCCGAACTTGTTTATTGAAGAACACATTCTAAACTCAAATCTTAATGTATCTTCGTTGAAATCTAGAGTCGTTATAGCTGTTGATTTTATTAGATCATACATCTTCTGGTTCTTGTTGGCCTGACTATACTTGCTGTGTGTAGATCGTCCCATCATTCATAGAGACATAAAGTCTTCCAACATTCTCTTGACCGAGAATTATAGAGCCAAAGTTGCGGACTTTGGCTTTGCAAGACTTGCAGCAGACGGCGATGCGACCCATGTGTCCACTCAAGTCAAAGGAACTGCTGGCTACTTAGACCCAGAATATCTCAAAACTTACCAACTGACTGAAAAGAGTGATATCTATTCATTTGGTGTTTTGTTGGTTGAATTAGTCACAGGCAGGCGCCCCATAGAACCCAAACGAGAACTTGACCAGCGGATCACTCCAAAATGGGTGAGCCTTTTTCTCTCTTCGTTTCACTACTAGTTTAGGATTATATATCATAACCATAGAGAATACGTGAGGATTGTTGAGAGGGAGTTCCATATCATAACCGTAGAGAATACGTTGAGGATTGTTGAGAGGGAGTTCCAAATTGGTTAATTAAGGAAATGATCATGAGTTTATAAGTAAGGAATATTATCTCTATTGGTACGAGATTTTTTGGGGAAACCAAAAAGAGCCACGAGAGCTTATGCTCAAAGTGAATAATATTATACCATTGTATAGGTTGGTGGATCTTAACCGAATATAGTCCATAGTAGTTACATAAATGACACTATCTCTATTGGTACGTAGTTTTTAGTAGAATCTAAAAAATAGCTCATGTGAATACAAATTCAATCTAAATAAGATCGCACCATGGTGAGAAACTAATTATCATTTGTTCTTCACACACTGCTCTGCGTTGACATAAATGTAGGCCATGAAGAAGTTTTGTGAAGGAGAGGCCAGCACACTTTTGGACCCAAATCTAGAGCAAACAACTGCAAATCATTCAGCAGCGGAAAAGATTCTGGAGTTGGCCTTACAATGCTTGGCTCCCCGGAGGCATAGTCGGCCGAGCATGAGGAGATGTGCAGAGATACTCTGGAGCATACGCAAGGACCACAGAGATCTGACAGCTCCTGATCTCCGTTCATTCTCTACAAACTCACAAAGGAGTGCCTCGGTAAGAGAAAAATGAAACTCAAATCTTGGTTCATAATCATGATAAATTTGGAATTCTTGATCCTATGAAGAACAAAGCTCTCTTAGAATCTCTATTCCACGAAGCAAGCAGATTCCCAGAACGTATGTTTCTCTTCTTATGTTCATTGGTGAGATTATATTCGTTTGGCTTGTGTGCTAGCTGCATTCTTTAGCTTCATTTATGCTGCTAATAATACTCTCTTTTGCTTGTAAAGTTGTGTATTTAGCAACCTCAAGGATGATTGATTGGTTTCTGTTGTGACTTTGTTCATCATTTGTGACTGAATAGTTAATAAATTGATTAGGAAATGGAGAAACTTCTGCCC

mRNA sequence

ATTAATAGGTACCGCCATTTCTGAGAGTCTTGTTGGTTTTACTCTGTTAGCGCCAATGACGAGCTCAAGTCCGTGGCACGGCGGTGAGCGGCGGTATTCTAGCTCCAGCGGGGAGCGGCGGTATTCTAGTTCCAGCGGTGAGCGGCGGAATTCTAGCTCTGTCAGTGAGAGGCGGAACTCCAGCTCCCGTTGTAGTCGATCGCCTTACCTTTCTTACTTGCCCAGTCCCGATGTGTCCGCTGGTATGGAATCCCGTTCCCGACTTAATCCGGTGAAAGCCGCTGCACGGTCCGTCGCCGGAGCTTTCATTACTTGCTTTACACCGCCGGAGAAGGAAACTTCGCCGGGTTTTGGTATTGGTAGCGAATTTCGTCATTCTTCAGATGCTTCCAGTCATAATAGCAAAAGAAATCAAGGATCTAGGCGAGAAACCCACTTCAGTTCAAATCTAGCTGCCCATGATCGAGAGCCTGGATCTTTAAGGATCAGCATGGAAGAAATCCGCAAGGCTACGAAGAACTTCTCCGCATCTTCAAAAATTGGCCAAGGGGGTTTTGGGGCTGTCTACAAGGGGAAACTTGATGGAGTTCTTGTGGCAATAAAACGTGCCAAGAAGAGTGTATATGATAATAATTTGGGCGTGGAGTTTCAAAGCGAGATCCAAACACTTGCACAAGTAGAACATTTAAATCTGGTTAAGTTCTATGGATATCTGGAACATGAAGATGAAAGAATCGTCATTGTTGAGTATGTGCCGAATGGGACACTCAGAGAGCACTTAGAATGCATCCATGGCACTACTCTTGACCTTGCTACGAGGCTCGCCATTGCAACCGATGTGGCTCATGCAATAACGTATCTTCATATGTATACAGATCGTCCCATCATTCATAGAGACATAAAGTCTTCCAACATTCTCTTGACCGAGAATTATAGAGCCAAAGTTGCGGACTTTGGCTTTGCAAGACTTGCAGCAGACGGCGATGCGACCCATGTGTCCACTCAAGTCAAAGGAACTGCTGGCTACTTAGACCCAGAATATCTCAAAACTTACCAACTGACTGAAAAGAGTGATATCTATTCATTTGGTGTTTTGTTGGTTGAATTAGTCACAGGCAGGCGCCCCATAGAACCCAAACGAGAACTTGACCAGCGGATCACTCCAAAATGGGCCATGAAGAAGTTTTGTGAAGGAGAGGCCAGCACACTTTTGGACCCAAATCTAGAGCAAACAACTGCAAATCATTCAGCAGCGGAAAAGATTCTGGAGTTGGCCTTACAATGCTTGGCTCCCCGGAGGCATAGTCGGCCGAGCATGAGGAGATGTGCAGAGATACTCTGGAGCATACGCAAGGACCACAGAGATCTGACAGCTCCTGATCTCCGTTCATTCTCTACAAACTCACAAAGGAGTGCCTCGGTAAGAGAAAAATGAAACTCAAATCTTGGTTCATAATCATGATAAATTTGGAATTCTTGATCCTATGAAGAACAAAGCTCTCTTAGAATCTCTATTCCACGAAGCAAGCAGATTCCCAGAACGTATGTTTCTCTTCTTATGTTCATTGGTGAGATTATATTCGTTTGGCTTGTGTGCTAGCTGCATTCTTTAGCTTCATTTATGCTGCTAATAATACTCTCTTTTGCTTGTAAAGTTGTGTATTTAGCAACCTCAAGGATGATTGATTGGTTTCTGTTGTGACTTTGTTCATCATTTGTGACTGAATAGTTAATAAATTGATTAGGAAATGGAGAAACTTCTGCCC

Coding sequence (CDS)

ATGACGAGCTCAAGTCCGTGGCACGGCGGTGAGCGGCGGTATTCTAGCTCCAGCGGGGAGCGGCGGTATTCTAGTTCCAGCGGTGAGCGGCGGAATTCTAGCTCTGTCAGTGAGAGGCGGAACTCCAGCTCCCGTTGTAGTCGATCGCCTTACCTTTCTTACTTGCCCAGTCCCGATGTGTCCGCTGGTATGGAATCCCGTTCCCGACTTAATCCGGTGAAAGCCGCTGCACGGTCCGTCGCCGGAGCTTTCATTACTTGCTTTACACCGCCGGAGAAGGAAACTTCGCCGGGTTTTGGTATTGGTAGCGAATTTCGTCATTCTTCAGATGCTTCCAGTCATAATAGCAAAAGAAATCAAGGATCTAGGCGAGAAACCCACTTCAGTTCAAATCTAGCTGCCCATGATCGAGAGCCTGGATCTTTAAGGATCAGCATGGAAGAAATCCGCAAGGCTACGAAGAACTTCTCCGCATCTTCAAAAATTGGCCAAGGGGGTTTTGGGGCTGTCTACAAGGGGAAACTTGATGGAGTTCTTGTGGCAATAAAACGTGCCAAGAAGAGTGTATATGATAATAATTTGGGCGTGGAGTTTCAAAGCGAGATCCAAACACTTGCACAAGTAGAACATTTAAATCTGGTTAAGTTCTATGGATATCTGGAACATGAAGATGAAAGAATCGTCATTGTTGAGTATGTGCCGAATGGGACACTCAGAGAGCACTTAGAATGCATCCATGGCACTACTCTTGACCTTGCTACGAGGCTCGCCATTGCAACCGATGTGGCTCATGCAATAACGTATCTTCATATGTATACAGATCGTCCCATCATTCATAGAGACATAAAGTCTTCCAACATTCTCTTGACCGAGAATTATAGAGCCAAAGTTGCGGACTTTGGCTTTGCAAGACTTGCAGCAGACGGCGATGCGACCCATGTGTCCACTCAAGTCAAAGGAACTGCTGGCTACTTAGACCCAGAATATCTCAAAACTTACCAACTGACTGAAAAGAGTGATATCTATTCATTTGGTGTTTTGTTGGTTGAATTAGTCACAGGCAGGCGCCCCATAGAACCCAAACGAGAACTTGACCAGCGGATCACTCCAAAATGGGCCATGAAGAAGTTTTGTGAAGGAGAGGCCAGCACACTTTTGGACCCAAATCTAGAGCAAACAACTGCAAATCATTCAGCAGCGGAAAAGATTCTGGAGTTGGCCTTACAATGCTTGGCTCCCCGGAGGCATAGTCGGCCGAGCATGAGGAGATGTGCAGAGATACTCTGGAGCATACGCAAGGACCACAGAGATCTGACAGCTCCTGATCTCCGTTCATTCTCTACAAACTCACAAAGGAGTGCCTCGGTAAGAGAAAAATGA

Protein sequence

MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQGSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK
Homology
BLAST of Carg20990 vs. NCBI nr
Match: KAG7019016.1 (Calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 885.9 bits (2288), Expect = 1.4e-253
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV 60
           MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV
Sbjct: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV 60

Query: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120
           SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ
Sbjct: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120

Query: 121 GSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180
           GSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV
Sbjct: 121 GSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180

Query: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240
           AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE
Sbjct: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240

Query: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300
           HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF
Sbjct: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300

Query: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360
           GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP
Sbjct: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360

Query: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420
           KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS
Sbjct: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420

Query: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK
Sbjct: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 459

BLAST of Carg20990 vs. NCBI nr
Match: KAG6582621.1 (Calmodulin-binding receptor-like cytoplasmic kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 884.4 bits (2284), Expect = 4.2e-253
Identity = 458/459 (99.78%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV 60
           MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV
Sbjct: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV 60

Query: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120
           SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ
Sbjct: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120

Query: 121 GSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180
           GSRRETHFSSNLAAHDREPGSLRIS+EEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV
Sbjct: 121 GSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180

Query: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240
           AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE
Sbjct: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240

Query: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300
           HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF
Sbjct: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300

Query: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360
           GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP
Sbjct: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360

Query: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420
           KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS
Sbjct: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420

Query: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK
Sbjct: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 459

BLAST of Carg20990 vs. NCBI nr
Match: XP_022924490.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita moschata])

HSP 1 Score: 881.7 bits (2277), Expect = 2.7e-252
Identity = 457/459 (99.56%), Postives = 458/459 (99.78%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV 60
           MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCS SPYLSYLPSPDV
Sbjct: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSGSPYLSYLPSPDV 60

Query: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120
           SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ
Sbjct: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120

Query: 121 GSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180
           GSRRETHFSSNLAAHDREPGSLRIS+EEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV
Sbjct: 121 GSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180

Query: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240
           AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE
Sbjct: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240

Query: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300
           HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF
Sbjct: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300

Query: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360
           GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP
Sbjct: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360

Query: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420
           KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS
Sbjct: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420

Query: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK
Sbjct: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 459

BLAST of Carg20990 vs. NCBI nr
Match: XP_023526304.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 864.0 bits (2231), Expect = 5.8e-247
Identity = 452/468 (96.58%), Postives = 454/468 (97.01%), Query Frame = 0

Query: 1   MTSSSPWHG---------GERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPY 60
           MTSSSPWHG         GERRYSSSSGERRYSSSSGERRNSSSVSERRNS SR SRSPY
Sbjct: 1   MTSSSPWHGGERRNSSSSGERRYSSSSGERRYSSSSGERRNSSSVSERRNSCSRGSRSPY 60

Query: 61  LSYLPSPDVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDA 120
           LSYLPSPDVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIG EFRHSSDA
Sbjct: 61  LSYLPSPDVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGREFRHSSDA 120

Query: 121 SSHNSKRNQGSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVY 180
           SSHNSKRNQGSRRETHFSSNLAAHDREPGSLRIS+EEIRKATKNFSASSKIGQGGFGAVY
Sbjct: 121 SSHNSKRNQGSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVY 180

Query: 181 KGKLDGVLVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVE 240
           KGKLDGVLVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVE
Sbjct: 181 KGKLDGVLVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVE 240

Query: 241 YVPNGTLREHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTE 300
           YVPNGTLREHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTE
Sbjct: 241 YVPNGTLREHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTE 300

Query: 301 NYRAKVADFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVEL 360
           NYRAKVADFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVEL
Sbjct: 301 NYRAKVADFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVEL 360

Query: 361 VTGRRPIEPKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCL 420
           VTGRRPIEPKRELDQRITPKWAMKKFCEGEAST+LDPNLEQT ANHSA EKILELALQCL
Sbjct: 361 VTGRRPIEPKRELDQRITPKWAMKKFCEGEASTILDPNLEQTAANHSAVEKILELALQCL 420

Query: 421 APRRHSRPSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           APRRHSRPSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK
Sbjct: 421 APRRHSRPSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 468

BLAST of Carg20990 vs. NCBI nr
Match: XP_022980487.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita maxima])

HSP 1 Score: 851.7 bits (2199), Expect = 3.0e-243
Identity = 442/459 (96.30%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV 60
           MTSSSPWHG         GERRYSSSSGERRNSSSVSERRN SSRCSRSPYLSYLPSPDV
Sbjct: 1   MTSSSPWHG---------GERRYSSSSGERRNSSSVSERRNPSSRCSRSPYLSYLPSPDV 60

Query: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120
           SAGMESRSRLNPVK+AARSVAGAFITCFTPPEKETSPGFGIGSEFRHS DASSHNSKRNQ
Sbjct: 61  SAGMESRSRLNPVKSAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSLDASSHNSKRNQ 120

Query: 121 GSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180
           GSRRETHFSSNLAAHDREPGSLRIS+EEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV
Sbjct: 121 GSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180

Query: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240
           AIKRAKKSVYDNNLGVEF+SEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE
Sbjct: 181 AIKRAKKSVYDNNLGVEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240

Query: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300
           HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF
Sbjct: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300

Query: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360
           GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP
Sbjct: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360

Query: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420
           KRELDQRITPKWAMKKFCEGEAST+LDPNLEQT ANHSA EKILELALQCLAPRRHSRPS
Sbjct: 361 KRELDQRITPKWAMKKFCEGEASTILDPNLEQTAANHSAVEKILELALQCLAPRRHSRPS 420

Query: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK
Sbjct: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 450

BLAST of Carg20990 vs. ExPASy Swiss-Prot
Match: Q8VZJ9 (Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CRCK2 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.4e-121
Identity = 244/416 (58.65%), Postives = 301/416 (72.36%), Query Frame = 0

Query: 30  RRNSSSVSERRNSSSRCSRSPYL--SYLPSPDVSAGMESRSRLNPVKAAARSVAGAFITC 89
           RR+S +V     +SS  S + +   S+  +P       +R+  +   + AR ++G FI C
Sbjct: 5   RRHSYTVGSPATTSSNYSNTTFTDRSFPTTP-------ARTSTDTTTSIARRISGIFINC 64

Query: 90  FTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQGSRRETHFSSNLAAHDREPGSLRISME 149
           FTPP+        + S +  +S +SS N+ R++ S   +        +  E    R + +
Sbjct: 65  FTPPD-------SVSSNYIDNSKSSSDNNIRSRRSSTGSSSVQRSYGNANETEHTRFTFD 124

Query: 150 EIRKATKNFSASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLG--VEFQSEIQT 209
           EI  ATKNFS S +IGQGGFG VYK KL DG   A+KRAKKS++D+  G   EF SEIQT
Sbjct: 125 EIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKRAKKSMHDDRQGADAEFMSEIQT 184

Query: 210 LAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAIATDVAH 269
           LAQV HL+LVK+YG++ H DE+I++VEYV NGTLR+HL+C  G TLD+ATRL IATDVAH
Sbjct: 185 LAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHLDCKEGKTLDMATRLDIATDVAH 244

Query: 270 AITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLAADGD--ATHVSTQVKGTA 329
           AITYLHMYT  PIIHRDIKSSNILLTENYRAKVADFGFARLA D D  ATHVSTQVKGTA
Sbjct: 245 AITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGFARLAPDTDSGATHVSTQVKGTA 304

Query: 330 GYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFCEGEA 389
           GYLDPEYL TYQLTEKSD+YSFGVLLVEL+TGRRPIE  R   +RIT +WA+KKF  G+ 
Sbjct: 305 GYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIELSRGQKERITIRWAIKKFTSGDT 364

Query: 390 STLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSIRKDHRDL 439
            ++LDP LEQ +AN+ A EK+LE+A QCLAP R SRPSM++C+EILW IRKD+R+L
Sbjct: 365 ISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPSMKKCSEILWGIRKDYREL 406

BLAST of Carg20990 vs. ExPASy Swiss-Prot
Match: Q9FIL7 (Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CRCK1 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.0e-97
Identity = 182/342 (53.22%), Postives = 252/342 (73.68%), Query Frame = 0

Query: 98  GFGIGSEFRHSSDASSHNSKRNQGSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFS 157
           G+   S F  S++       R  GSR ++    + ++     G +  S  E+++AT NFS
Sbjct: 89  GYSSPSSFGRSTERKVSGQYRFSGSRFQSPGKDSSSSKSWHQGPVIFSFGELQRATANFS 148

Query: 158 ASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKF 217
           +  +IG+GGFG V+KGKL DG +VAIKRA+K+ Y  +  +EF++EI TL+++EH+NLVK 
Sbjct: 149 SVHQIGEGGFGTVFKGKLDDGTIVAIKRARKNNYGKSWLLEFKNEIYTLSKIEHMNLVKL 208

Query: 218 YGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRP 277
           YG+LEH DE++++VEYV NG LREHL+ + G  L++A RL IA DVAHA+TYLH YTD P
Sbjct: 209 YGFLEHGDEKVIVVEYVANGNLREHLDGLRGNRLEMAERLEIAIDVAHALTYLHTYTDSP 268

Query: 278 IIHRDIKSSNILLTENYRAKVADFGFARLAA-DGDATHVSTQVKGTAGYLDPEYLKTYQL 337
           IIHRDIK+SNIL+T   RAKVADFGFARL + D  ATH+STQVKG+AGY+DP+YL+T+QL
Sbjct: 269 IIHRDIKASNILITNKLRAKVADFGFARLVSEDLGATHISTQVKGSAGYVDPDYLRTFQL 328

Query: 338 TEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTA 397
           T+KSD+YSFGVLLVE++TGRRPIE KR    R+T KWA+++  + EA  ++DP L++  A
Sbjct: 329 TDKSDVYSFGVLLVEILTGRRPIELKRPRKDRLTVKWALRRLKDDEAVLIMDPFLKRNRA 388

Query: 398 NHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSIRKDHRD 438
               AEK+L LA +C+ P R +RP+M+  AE LW+IR++ ++
Sbjct: 389 AIEVAEKMLRLASECVTPTRATRPAMKGIAEKLWAIRREMKE 430

BLAST of Carg20990 vs. ExPASy Swiss-Prot
Match: Q9ASQ5 (Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRCK3 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 5.4e-86
Identity = 164/298 (55.03%), Postives = 209/298 (70.13%), Query Frame = 0

Query: 140 GSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLGVEF 199
           G L ++M +I  AT NF+ S +IG+GGFG V+KG L DG +VAIKRAKK  ++ NL  EF
Sbjct: 209 GPLNLTMSQINTATGNFADSHQIGEGGFGVVFKGVLDDGQVVAIKRAKKEHFE-NLRTEF 268

Query: 200 QSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAI 259
           +SE+  L+++ H NLVK  GY++  DER++I EYV NGTLR+HL+   GT L+   RL I
Sbjct: 269 KSEVDLLSKIGHRNLVKLLGYVDKGDERLIITEYVRNGTLRDHLDGARGTKLNFNQRLEI 328

Query: 260 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLA-ADGDATHVSTQ 319
             DV H +TYLH Y +R IIHRDIKSSNILLT++ RAKVADFGFAR    D + TH+ TQ
Sbjct: 329 VIDVCHGLTYLHSYAERQIIHRDIKSSNILLTDSMRAKVADFGFARGGPTDSNQTHILTQ 388

Query: 320 VKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKF 379
           VKGT GYLDPEY+KTY LT KSD+YSFG+LLVE++TGRRP+E KR  D+RIT +WA  K+
Sbjct: 389 VKGTVGYLDPEYMKTYHLTAKSDVYSFGILLVEILTGRRPVEAKRLPDERITVRWAFDKY 448

Query: 380 CEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSIRKDH 436
            EG    L+DPN  +   +     K+  LA QC AP +  RP M    + LW+IR  +
Sbjct: 449 NEGRVFELVDPNARE-RVDEKILRKMFSLAFQCAAPTKKERPDMEAVGKQLWAIRSSY 504

BLAST of Carg20990 vs. ExPASy Swiss-Prot
Match: Q9LT96 (Probable leucine-rich repeat receptor-like protein kinase At5g49770 OS=Arabidopsis thaliana OX=3702 GN=At5g49770 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.6e-61
Identity = 128/293 (43.69%), Postives = 185/293 (63.14%), Query Frame = 0

Query: 140 GSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLGVEF 199
           G+   + EE+ K T NFS ++ +G GG+G VYKG L +G ++AIKRA++         EF
Sbjct: 618 GTKAFTFEELSKCTNNFSDANDVGGGGYGQVYKGTLPNGQVIAIKRAQQGSMQG--AFEF 677

Query: 200 QSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAI 259
           ++EI+ L++V H N+VK  G+   + E++++ EY+PNG+LR+ L   +G  LD   RL I
Sbjct: 678 KTEIELLSRVHHKNVVKLLGFCFDQKEQMLVYEYIPNGSLRDGLSGKNGVKLDWTRRLKI 737

Query: 260 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLAADGDATHVSTQV 319
           A      + YLH   D PIIHRD+KS+NILL E+  AKVADFG ++L  D +  HV+TQV
Sbjct: 738 ALGSGKGLAYLHELADPPIIHRDVKSNNILLDEHLTAKVADFGLSKLVGDPEKAHVTTQV 797

Query: 320 KGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFC 379
           KGT GYLDPEY  T QLTEKSD+Y FGV+++EL+TG+ PI+    + + +  K    +  
Sbjct: 798 KGTMGYLDPEYYMTNQLTEKSDVYGFGVVMLELLTGKSPIDRGSYVVKEVKKKMDKSRNL 857

Query: 380 EGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSI 432
             +   LLD  + Q + N    EK +++ALQC+ P   +RP+M    + L SI
Sbjct: 858 Y-DLQELLDTTIIQNSGNLKGFEKYVDVALQCVEPEGVNRPTMSEVVQELESI 907

BLAST of Carg20990 vs. ExPASy Swiss-Prot
Match: Q8GZ99 (Leucine-rich repeat receptor protein kinase HPCA1 OS=Arabidopsis thaliana OX=3702 GN=HPCA1 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.7e-61
Identity = 128/283 (45.23%), Postives = 183/283 (64.66%), Query Frame = 0

Query: 140 GSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLGVEF 199
           G+   + EE++K T NFS ++ +G GG+G VY+G L +G L+AIKRA++       G+EF
Sbjct: 615 GAKAFTFEELKKCTDNFSEANDVGGGGYGKVYRGILPNGQLIAIKRAQQGSLQG--GLEF 674

Query: 200 QSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAI 259
           ++EI+ L++V H N+V+  G+    +E++++ EY+ NG+L++ L    G  LD   RL I
Sbjct: 675 KTEIELLSRVHHKNVVRLLGFCFDRNEQMLVYEYISNGSLKDSLSGKSGIRLDWTRRLKI 734

Query: 260 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLAADGDATHVSTQV 319
           A      + YLH   D PIIHRDIKS+NILL EN  AKVADFG ++L  D + THV+TQV
Sbjct: 735 ALGSGKGLAYLHELADPPIIHRDIKSNNILLDENLTAKVADFGLSKLVGDPEKTHVTTQV 794

Query: 320 KGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFC 379
           KGT GYLDPEY  T QLTEKSD+Y FGV+L+EL+TGR PIE  + + + +  K    +  
Sbjct: 795 KGTMGYLDPEYYMTNQLTEKSDVYGFGVVLLELLTGRSPIERGKYVVREVKTKMNKSRSL 854

Query: 380 EGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSM 422
             +   LLD  +  ++ N    EK ++LAL+C+     +RPSM
Sbjct: 855 Y-DLQELLDTTIIASSGNLKGFEKYVDLALRCVEEEGVNRPSM 894

BLAST of Carg20990 vs. ExPASy TrEMBL
Match: A0A6J1EF63 (calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucurbita moschata OX=3662 GN=LOC111431968 PE=3 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.3e-252
Identity = 457/459 (99.56%), Postives = 458/459 (99.78%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV 60
           MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCS SPYLSYLPSPDV
Sbjct: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSGSPYLSYLPSPDV 60

Query: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120
           SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ
Sbjct: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120

Query: 121 GSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180
           GSRRETHFSSNLAAHDREPGSLRIS+EEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV
Sbjct: 121 GSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180

Query: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240
           AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE
Sbjct: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240

Query: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300
           HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF
Sbjct: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300

Query: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360
           GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP
Sbjct: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360

Query: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420
           KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS
Sbjct: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420

Query: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK
Sbjct: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 459

BLAST of Carg20990 vs. ExPASy TrEMBL
Match: A0A6J1IRI1 (calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucurbita maxima OX=3661 GN=LOC111479865 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.5e-243
Identity = 442/459 (96.30%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSPYLSYLPSPDV 60
           MTSSSPWHG         GERRYSSSSGERRNSSSVSERRN SSRCSRSPYLSYLPSPDV
Sbjct: 1   MTSSSPWHG---------GERRYSSSSGERRNSSSVSERRNPSSRCSRSPYLSYLPSPDV 60

Query: 61  SAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQ 120
           SAGMESRSRLNPVK+AARSVAGAFITCFTPPEKETSPGFGIGSEFRHS DASSHNSKRNQ
Sbjct: 61  SAGMESRSRLNPVKSAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSLDASSHNSKRNQ 120

Query: 121 GSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180
           GSRRETHFSSNLAAHDREPGSLRIS+EEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV
Sbjct: 121 GSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVLV 180

Query: 181 AIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240
           AIKRAKKSVYDNNLGVEF+SEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE
Sbjct: 181 AIKRAKKSVYDNNLGVEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLRE 240

Query: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300
           HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF
Sbjct: 241 HLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADF 300

Query: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360
           GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP
Sbjct: 301 GFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 360

Query: 361 KRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPS 420
           KRELDQRITPKWAMKKFCEGEAST+LDPNLEQT ANHSA EKILELALQCLAPRRHSRPS
Sbjct: 361 KRELDQRITPKWAMKKFCEGEASTILDPNLEQTAANHSAVEKILELALQCLAPRRHSRPS 420

Query: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK
Sbjct: 421 MRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 450

BLAST of Carg20990 vs. ExPASy TrEMBL
Match: A0A0A0L7F9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121770 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.6e-221
Identity = 409/460 (88.91%), Postives = 424/460 (92.17%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSP-YLSYLPSPD 60
           MTS SPWHG         GERR S+SSGERRNSSSVSERRNSSSR SR+P Y  Y PSPD
Sbjct: 1   MTSPSPWHG---------GERRNSTSSGERRNSSSVSERRNSSSRGSRTPVYFPYSPSPD 60

Query: 61  VSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRN 120
           VS GMESRSRLNPVKAAARSVAGAF +CFTPPEKET P  G GSEFR+SSDASSHN +RN
Sbjct: 61  VSVGMESRSRLNPVKAAARSVAGAFTSCFTPPEKETPPSLGFGSEFRNSSDASSHNGRRN 120

Query: 121 QGSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL 180
           QGSRRE   SSNL +HDREPGSLRI++EEIRKATKNFS SSKIGQGGFG+VYKGKLDGVL
Sbjct: 121 QGSRREPRSSSNL-SHDREPGSLRINIEEIRKATKNFSVSSKIGQGGFGSVYKGKLDGVL 180

Query: 181 VAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLR 240
           VAIKRAKKSVYDNNLG+EF+SEIQTLAQVEHLNLVKFYGYLEH+DERIVIVEYVPNGTLR
Sbjct: 181 VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHQDERIVIVEYVPNGTLR 240

Query: 241 EHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD 300
           EHLECIHGT LDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD
Sbjct: 241 EHLECIHGTVLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD 300

Query: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360
           FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE
Sbjct: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360

Query: 361 PKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRP 420
           PKREL+QRITPKWAMKKF EGEAS +LDPNLEQT ANH A EKILELALQCLAPRRHSRP
Sbjct: 361 PKRELEQRITPKWAMKKFSEGEASAILDPNLEQTEANHLAVEKILELALQCLAPRRHSRP 420

Query: 421 SMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           SMRRCAEILW IRKDHRDL+APD RSFSTNSQRSASVREK
Sbjct: 421 SMRRCAEILWRIRKDHRDLSAPDFRSFSTNSQRSASVREK 450

BLAST of Carg20990 vs. ExPASy TrEMBL
Match: A0A5D3D3L0 (Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004790 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.6e-220
Identity = 409/460 (88.91%), Postives = 422/460 (91.74%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSP-YLSYLPSPD 60
           MTS SPWHG         GERR S+SSGERRNSSSVS+RRNSSSR SR+P Y  Y PSPD
Sbjct: 1   MTSPSPWHG---------GERRNSTSSGERRNSSSVSDRRNSSSRGSRTPVYFPYSPSPD 60

Query: 61  VSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRN 120
           VS GMESRSRLNPVKAAARS AGAF +CFTPPEKET    G GSE R+SSDASSHN KRN
Sbjct: 61  VSTGMESRSRLNPVKAAARSFAGAFTSCFTPPEKETPRSLGFGSELRNSSDASSHNGKRN 120

Query: 121 QGSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL 180
           QGSRRE   SSNL AHDREPGSLRI++EEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL
Sbjct: 121 QGSRREPRSSSNL-AHDREPGSLRINIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL 180

Query: 181 VAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLR 240
           VAIKRAKKSVYDNNLG+EF+SEIQTLAQVEHLNLVKFYGYLEH+DERIVIVEYVPNGTLR
Sbjct: 181 VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHQDERIVIVEYVPNGTLR 240

Query: 241 EHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD 300
           EHLECIHGT LDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD
Sbjct: 241 EHLECIHGTVLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD 300

Query: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360
           FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE
Sbjct: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360

Query: 361 PKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRP 420
           PKREL+QRITPKWAMKKF EGEAS +LDPNLEQT ANH A EKILELALQCLAPRRHSRP
Sbjct: 361 PKRELEQRITPKWAMKKFSEGEASAILDPNLEQTEANHLAVEKILELALQCLAPRRHSRP 420

Query: 421 SMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           SMRRCAEILW IRKDHRDL+APD RSFSTNSQRSASVREK
Sbjct: 421 SMRRCAEILWRIRKDHRDLSAPDFRSFSTNSQRSASVREK 450

BLAST of Carg20990 vs. ExPASy TrEMBL
Match: A0A5A7U166 (Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001790 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.9e-219
Identity = 409/460 (88.91%), Postives = 421/460 (91.52%), Query Frame = 0

Query: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSRSP-YLSYLPSPD 60
           MTS SPWHG         GERR S+SSGERRNSSSVSERRNSSSR SR+P Y  Y PSPD
Sbjct: 1   MTSPSPWHG---------GERRNSTSSGERRNSSSVSERRNSSSRGSRTPVYFPYSPSPD 60

Query: 61  VSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKRN 120
           VS GMESRSR NPVKAAARS AGAF +CFTPPEKET    G GSE R+SSDASSHN KRN
Sbjct: 61  VSTGMESRSRHNPVKAAARSFAGAFTSCFTPPEKETPRSLGFGSELRNSSDASSHNGKRN 120

Query: 121 QGSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL 180
           QGSRRE   SSNL AHDREPGSLRI++EEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL
Sbjct: 121 QGSRREPRSSSNL-AHDREPGSLRINIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL 180

Query: 181 VAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLR 240
           VAIKRAKKSVYDNNLG+EF+SEIQTLAQVEHLNLVKFYGYLEH+DERIVIVEYVPNGTLR
Sbjct: 181 VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHQDERIVIVEYVPNGTLR 240

Query: 241 EHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD 300
           EHLECIHGT LDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD
Sbjct: 241 EHLECIHGTVLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD 300

Query: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360
           FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE
Sbjct: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360

Query: 361 PKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRP 420
           PKREL+QRITPKWAMKKF EGEAS +LDPNLEQT ANH A EKILELALQCLAPRRHSRP
Sbjct: 361 PKRELEQRITPKWAMKKFSEGEASAILDPNLEQTEANHLAVEKILELALQCLAPRRHSRP 420

Query: 421 SMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 460
           SMRRCAEILW IRKDHRDL+APD RSFSTNSQRSASVREK
Sbjct: 421 SMRRCAEILWRIRKDHRDLSAPDFRSFSTNSQRSASVREK 450

BLAST of Carg20990 vs. TAIR 10
Match: AT4G00330.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 )

HSP 1 Score: 438.0 bits (1125), Expect = 9.6e-123
Identity = 244/416 (58.65%), Postives = 301/416 (72.36%), Query Frame = 0

Query: 30  RRNSSSVSERRNSSSRCSRSPYL--SYLPSPDVSAGMESRSRLNPVKAAARSVAGAFITC 89
           RR+S +V     +SS  S + +   S+  +P       +R+  +   + AR ++G FI C
Sbjct: 5   RRHSYTVGSPATTSSNYSNTTFTDRSFPTTP-------ARTSTDTTTSIARRISGIFINC 64

Query: 90  FTPPEKETSPGFGIGSEFRHSSDASSHNSKRNQGSRRETHFSSNLAAHDREPGSLRISME 149
           FTPP+        + S +  +S +SS N+ R++ S   +        +  E    R + +
Sbjct: 65  FTPPD-------SVSSNYIDNSKSSSDNNIRSRRSSTGSSSVQRSYGNANETEHTRFTFD 124

Query: 150 EIRKATKNFSASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLG--VEFQSEIQT 209
           EI  ATKNFS S +IGQGGFG VYK KL DG   A+KRAKKS++D+  G   EF SEIQT
Sbjct: 125 EIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKRAKKSMHDDRQGADAEFMSEIQT 184

Query: 210 LAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAIATDVAH 269
           LAQV HL+LVK+YG++ H DE+I++VEYV NGTLR+HL+C  G TLD+ATRL IATDVAH
Sbjct: 185 LAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHLDCKEGKTLDMATRLDIATDVAH 244

Query: 270 AITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLAADGD--ATHVSTQVKGTA 329
           AITYLHMYT  PIIHRDIKSSNILLTENYRAKVADFGFARLA D D  ATHVSTQVKGTA
Sbjct: 245 AITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGFARLAPDTDSGATHVSTQVKGTA 304

Query: 330 GYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFCEGEA 389
           GYLDPEYL TYQLTEKSD+YSFGVLLVEL+TGRRPIE  R   +RIT +WA+KKF  G+ 
Sbjct: 305 GYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIELSRGQKERITIRWAIKKFTSGDT 364

Query: 390 STLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSIRKDHRDL 439
            ++LDP LEQ +AN+ A EK+LE+A QCLAP R SRPSM++C+EILW IRKD+R+L
Sbjct: 365 ISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPSMKKCSEILWGIRKDYREL 406

BLAST of Carg20990 vs. TAIR 10
Match: AT5G58940.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 )

HSP 1 Score: 357.1 bits (915), Expect = 2.2e-98
Identity = 182/342 (53.22%), Postives = 252/342 (73.68%), Query Frame = 0

Query: 98  GFGIGSEFRHSSDASSHNSKRNQGSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFS 157
           G+   S F  S++       R  GSR ++    + ++     G +  S  E+++AT NFS
Sbjct: 89  GYSSPSSFGRSTERKVSGQYRFSGSRFQSPGKDSSSSKSWHQGPVIFSFGELQRATANFS 148

Query: 158 ASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKF 217
           +  +IG+GGFG V+KGKL DG +VAIKRA+K+ Y  +  +EF++EI TL+++EH+NLVK 
Sbjct: 149 SVHQIGEGGFGTVFKGKLDDGTIVAIKRARKNNYGKSWLLEFKNEIYTLSKIEHMNLVKL 208

Query: 218 YGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRP 277
           YG+LEH DE++++VEYV NG LREHL+ + G  L++A RL IA DVAHA+TYLH YTD P
Sbjct: 209 YGFLEHGDEKVIVVEYVANGNLREHLDGLRGNRLEMAERLEIAIDVAHALTYLHTYTDSP 268

Query: 278 IIHRDIKSSNILLTENYRAKVADFGFARLAA-DGDATHVSTQVKGTAGYLDPEYLKTYQL 337
           IIHRDIK+SNIL+T   RAKVADFGFARL + D  ATH+STQVKG+AGY+DP+YL+T+QL
Sbjct: 269 IIHRDIKASNILITNKLRAKVADFGFARLVSEDLGATHISTQVKGSAGYVDPDYLRTFQL 328

Query: 338 TEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTA 397
           T+KSD+YSFGVLLVE++TGRRPIE KR    R+T KWA+++  + EA  ++DP L++  A
Sbjct: 329 TDKSDVYSFGVLLVEILTGRRPIELKRPRKDRLTVKWALRRLKDDEAVLIMDPFLKRNRA 388

Query: 398 NHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSIRKDHRD 438
               AEK+L LA +C+ P R +RP+M+  AE LW+IR++ ++
Sbjct: 389 AIEVAEKMLRLASECVTPTRATRPAMKGIAEKLWAIRREMKE 430

BLAST of Carg20990 vs. TAIR 10
Match: AT2G11520.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 )

HSP 1 Score: 319.7 bits (818), Expect = 3.8e-87
Identity = 164/298 (55.03%), Postives = 209/298 (70.13%), Query Frame = 0

Query: 140 GSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLGVEF 199
           G L ++M +I  AT NF+ S +IG+GGFG V+KG L DG +VAIKRAKK  ++ NL  EF
Sbjct: 209 GPLNLTMSQINTATGNFADSHQIGEGGFGVVFKGVLDDGQVVAIKRAKKEHFE-NLRTEF 268

Query: 200 QSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAI 259
           +SE+  L+++ H NLVK  GY++  DER++I EYV NGTLR+HL+   GT L+   RL I
Sbjct: 269 KSEVDLLSKIGHRNLVKLLGYVDKGDERLIITEYVRNGTLRDHLDGARGTKLNFNQRLEI 328

Query: 260 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLA-ADGDATHVSTQ 319
             DV H +TYLH Y +R IIHRDIKSSNILLT++ RAKVADFGFAR    D + TH+ TQ
Sbjct: 329 VIDVCHGLTYLHSYAERQIIHRDIKSSNILLTDSMRAKVADFGFARGGPTDSNQTHILTQ 388

Query: 320 VKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKF 379
           VKGT GYLDPEY+KTY LT KSD+YSFG+LLVE++TGRRP+E KR  D+RIT +WA  K+
Sbjct: 389 VKGTVGYLDPEYMKTYHLTAKSDVYSFGILLVEILTGRRPVEAKRLPDERITVRWAFDKY 448

Query: 380 CEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSIRKDH 436
            EG    L+DPN  +   +     K+  LA QC AP +  RP M    + LW+IR  +
Sbjct: 449 NEGRVFELVDPNARE-RVDEKILRKMFSLAFQCAAPTKKERPDMEAVGKQLWAIRSSY 504

BLAST of Carg20990 vs. TAIR 10
Match: AT5G49770.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 238.4 bits (607), Expect = 1.1e-62
Identity = 128/293 (43.69%), Postives = 185/293 (63.14%), Query Frame = 0

Query: 140 GSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLGVEF 199
           G+   + EE+ K T NFS ++ +G GG+G VYKG L +G ++AIKRA++         EF
Sbjct: 618 GTKAFTFEELSKCTNNFSDANDVGGGGYGQVYKGTLPNGQVIAIKRAQQGSMQG--AFEF 677

Query: 200 QSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAI 259
           ++EI+ L++V H N+VK  G+   + E++++ EY+PNG+LR+ L   +G  LD   RL I
Sbjct: 678 KTEIELLSRVHHKNVVKLLGFCFDQKEQMLVYEYIPNGSLRDGLSGKNGVKLDWTRRLKI 737

Query: 260 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLAADGDATHVSTQV 319
           A      + YLH   D PIIHRD+KS+NILL E+  AKVADFG ++L  D +  HV+TQV
Sbjct: 738 ALGSGKGLAYLHELADPPIIHRDVKSNNILLDEHLTAKVADFGLSKLVGDPEKAHVTTQV 797

Query: 320 KGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFC 379
           KGT GYLDPEY  T QLTEKSD+Y FGV+++EL+TG+ PI+    + + +  K    +  
Sbjct: 798 KGTMGYLDPEYYMTNQLTEKSDVYGFGVVMLELLTGKSPIDRGSYVVKEVKKKMDKSRNL 857

Query: 380 EGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSMRRCAEILWSI 432
             +   LLD  + Q + N    EK +++ALQC+ P   +RP+M    + L SI
Sbjct: 858 Y-DLQELLDTTIIQNSGNLKGFEKYVDVALQCVEPEGVNRPTMSEVVQELESI 907

BLAST of Carg20990 vs. TAIR 10
Match: AT5G49760.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.9e-62
Identity = 128/283 (45.23%), Postives = 183/283 (64.66%), Query Frame = 0

Query: 140 GSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKL-DGVLVAIKRAKKSVYDNNLGVEF 199
           G+   + EE++K T NFS ++ +G GG+G VY+G L +G L+AIKRA++       G+EF
Sbjct: 615 GAKAFTFEELKKCTDNFSEANDVGGGGYGKVYRGILPNGQLIAIKRAQQGSLQG--GLEF 674

Query: 200 QSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGTTLDLATRLAI 259
           ++EI+ L++V H N+V+  G+    +E++++ EY+ NG+L++ L    G  LD   RL I
Sbjct: 675 KTEIELLSRVHHKNVVRLLGFCFDRNEQMLVYEYISNGSLKDSLSGKSGIRLDWTRRLKI 734

Query: 260 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVADFGFARLAADGDATHVSTQV 319
           A      + YLH   D PIIHRDIKS+NILL EN  AKVADFG ++L  D + THV+TQV
Sbjct: 735 ALGSGKGLAYLHELADPPIIHRDIKSNNILLDENLTAKVADFGLSKLVGDPEKTHVTTQV 794

Query: 320 KGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELDQRITPKWAMKKFC 379
           KGT GYLDPEY  T QLTEKSD+Y FGV+L+EL+TGR PIE  + + + +  K    +  
Sbjct: 795 KGTMGYLDPEYYMTNQLTEKSDVYGFGVVLLELLTGRSPIERGKYVVREVKTKMNKSRSL 854

Query: 380 EGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSRPSM 422
             +   LLD  +  ++ N    EK ++LAL+C+     +RPSM
Sbjct: 855 Y-DLQELLDTTIIASSGNLKGFEKYVDLALRCVEEEGVNRPSM 894

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019016.11.4e-253100.00Calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita argyrosperma su... [more]
KAG6582621.14.2e-25399.78Calmodulin-binding receptor-like cytoplasmic kinase 2, partial [Cucurbita argyro... [more]
XP_022924490.12.7e-25299.56calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita moschata][more]
XP_023526304.15.8e-24796.58calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita pepo subsp. pep... [more]
XP_022980487.13.0e-24396.30calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8VZJ91.4e-12158.65Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX... [more]
Q9FIL73.0e-9753.22Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX... [more]
Q9ASQ55.4e-8655.03Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX... [more]
Q9LT961.6e-6143.69Probable leucine-rich repeat receptor-like protein kinase At5g49770 OS=Arabidops... [more]
Q8GZ992.7e-6145.23Leucine-rich repeat receptor protein kinase HPCA1 OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1EF631.3e-25299.56calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucurbita moschata OX=3... [more]
A0A6J1IRI11.5e-24396.30calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucurbita maxima OX=366... [more]
A0A0A0L7F94.6e-22188.91Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121... [more]
A0A5D3D3L06.6e-22088.91Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucumis melo var. makuw... [more]
A0A5A7U1661.9e-21988.91Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT4G00330.19.6e-12358.65calmodulin-binding receptor-like cytoplasmic kinase 2 [more]
AT5G58940.12.2e-9853.22calmodulin-binding receptor-like cytoplasmic kinase 1 [more]
AT2G11520.13.8e-8755.03calmodulin-binding receptor-like cytoplasmic kinase 3 [more]
AT5G49770.11.1e-6243.69Leucine-rich repeat protein kinase family protein [more]
AT5G49760.11.9e-6245.23Leucine-rich repeat protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 156..430
e-value: 6.3E-41
score: 152.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 158..426
e-value: 9.9E-52
score: 175.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 156..438
score: 39.69323
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 113..232
e-value: 6.1E-27
score: 95.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 233..450
e-value: 9.4E-58
score: 196.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..66
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 110..132
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 95..137
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..51
NoneNo IPR availablePANTHERPTHR45974RECEPTOR-LIKE PROTEIN 55coord: 33..448
NoneNo IPR availablePANTHERPTHR45974:SF34CALMODULIN-BINDING RECEPTOR-LIKE CYTOPLASMIC KINASE 2coord: 33..448
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 277..289
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 162..187
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 138..431

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20990-RACarg20990-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity