Carg20863 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20863
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionReticulon-like protein
LocationCarg_Chr06: 6893392 .. 6895604 (-)
RNA-Seq ExpressionCarg20863
SyntenyCarg20863
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCATCGAGTTGCTTGATTTTTCTTCTTCAGATCTTGGTCACTGCAATCGATCAATCATGGCGGACCACTCTGACAACGTGGAGTCGAAATCGGAGTCATTGATCGACAAGATAGTCGAGAAGATTCATGATCATGATTCTTCTTCGTCCGGATCAGACTCTGAAACCGAGGGATCCTTGAAGGAGAAGGTCTTCCGCCTTTTTGGAAGGGAGAGGCCAGTTCATCATGTCTTCGGCGGTGGAAAACGTACGGAATCTCACCTCTCTGATTTGAACTCATTGAATAATTGTTTTGTTTTTTTCTATTACATTTCCTAAGTTTTACACTGCTTCTATTCGTTTTATTCGACCTTCGTCTTGATTGGTGTTTCTTAGGCTTCTTTTTGCTCTTTGGATTCGTTCTAGCTTGTTTCCCCATTCTTTGCTTGGATTTAGAATATATAAATAATTAGAAATTATAGATCTGCTTGCTTTACCTTACTGTGACTCATTGATAGTTCAGATCGAGTGTACGCTATAATTGAGTGATTCATTGAGCTTCCAGAGCAGAGTATTTATTATATTCATTGATAGTTCAGATCGAGCGTACGCTATAATAGAGTGATTCATTGAGCTTCCAGAACAGATTATTTATTATTTTCTTCCCCGAACTTGTTATATTTGTTCCTTGCCCCGGTTTCGATACTTCTACTTCATTTTTTTCTGCGTTGCACATTTCTGATTTCACATTTGAATATGTTCTGATGACGATACTTGTGTTGCTTATGTCGTATGGTAATTTATTTTTTGTTTGGCGGTTGGTAATGTTGATGACCGTTTCTGGCCCCTGTTGCATATGCAGCTGCTGATTTATTCTTGTGGAGGAATAAGAAGATCTCAGCTGGTGTCCTTACTGGAGCCACTGTTATCTGGGTTTTCTTTGAACTGCTCGAGTGCCATCTGCTTACGTTTGTTTCTCGTTCTCTGATCCTCTCTCTTGGGCTCTTGTTTCTTTGGTCCAATGCCCACACATTTATCTACAAGTGAGTTACTGAATCTCAACTTCCTTTTATCAATAATTAAGTTTCTAGCGACTTCAAATTATGCTCTTCTTTCCACTGTTTAACAAATGTAGTTACAGGAGTCCACCCCGAATCCCAGAAGTTCAACTTCCTGCGGACCCCTTCCTGGAGTTTGCTTCAACTTTGAGGGCGGAAATTAATTGTGGTTTTGCATTATTGCGGGACATTGCTACCGGAAGAGATTTGAAGAAATTCATCACTGTACGTATCTGCTATTGATGCACTCTATTTGTCTTTTTATTGTGTGTATTTTACTTGTTGGGTTTATCTCAACTCTCAAGTAGCAACCGAGCCTGAACAAGTTGCAACTTAATATATATACCTTGATTGGACTACAGATTCGACCATAATACTCAATATGCTAATTTGTAGTCTCAGATATCGAAGTTAGAGTAATTGAATTTTATCTAGTTTATAGAAATGTTATGTCTCTGTATCAGGCAATTGCTGGGTTGTGGGTCCTGTCGATCGTGGGAAGCTGGTGCAATTTCTTGACGTTGTTCTACATAAGTAACGACAACTCGAACTCTATCTTCATATTTATAGCCATCTTTTTATCATCCTTTGTTGATTCTGACGTTTGACAACAACTTTGAAAACGCAGACTTTGTTTTGTTGCACACTGTTCCTGTTCTGTATGAGAAGTATGAAGATAAGATCGACCCATTAGCGGAAAAAGCATTAATTGAGTTCAAAAAGCACTACGCCGTGTTTGAAGAAAAGGTTTTGAGCAAGATCCCAAAAGGGCCATTGAAAGGTAAGAAGGCTTAAACTGGGTCTGGTTGGGTGTCTTCTTCCCTCGCAAAAAAGCGGTAACCACATGCTTATTGATCTCTTTCTCGTTGTCTTTTGGCTGCACTCATCCATCATAGTATAGTTGTTGTTCAATCACAAGGGTGGTTTTCGCAATTTAGTGGGTTTGTTCTGCAAATGTTGAATTTGTAAGTTATGTTATTAGTTGTATGGGAACTTTCATGAGGGGAAGTACTTTGGTTTGTGGTTAGCTTTAGCTAATTTTATATACTTTTTTTTTCTCTCTTTCGTTCCAATTTGGGTGATGCCTTGAGGGAAAGAATTATTAAACTAATTTTAATAATATTCAAACTTTTGAATTGAAA

mRNA sequence

TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCATCGAGTTGCTTGATTTTTCTTCTTCAGATCTTGGTCACTGCAATCGATCAATCATGGCGGACCACTCTGACAACGTGGAGTCGAAATCGGAGTCATTGATCGACAAGATAGTCGAGAAGATTCATGATCATGATTCTTCTTCGTCCGGATCAGACTCTGAAACCGAGGGATCCTTGAAGGAGAAGGTCTTCCGCCTTTTTGGAAGGGAGAGGCCAGTTCATCATGTCTTCGGCGGTGGAAAACCTGCTGATTTATTCTTGTGGAGGAATAAGAAGATCTCAGCTGGTGTCCTTACTGGAGCCACTGTTATCTGGGTTTTCTTTGAACTGCTCGAGTGCCATCTGCTTACGTTTGTTTCTCGTTCTCTGATCCTCTCTCTTGGGCTCTTGTTTCTTTGGTCCAATGCCCACACATTTATCTACAATTACAGGAGTCCACCCCGAATCCCAGAAGTTCAACTTCCTGCGGACCCCTTCCTGGAGTTTGCTTCAACTTTGAGGGCGGAAATTAATTGTGGTTTTGCATTATTGCGGGACATTGCTACCGGAAGAGATTTGAAGAAATTCATCACTGCAATTGCTGGGTTGTGGGTCCTGTCGATCGTGGGAAGCTGGTGCAATTTCTTGACGTTGTTCTACATAAACTTTGTTTTGTTGCACACTGTTCCTGTTCTGTATGAGAAGTATGAAGATAAGATCGACCCATTAGCGGAAAAAGCATTAATTGAGTTCAAAAAGCACTACGCCGTGTTTGAAGAAAAGGTTTTGAGCAAGATCCCAAAAGGGCCATTGAAAGGTAAGAAGGCTTAAACTGGGTCTGGTTGGGTGTCTTCTTCCCTCGCAAAAAAGCGGTAACCACATGCTTATTGATCTCTTTCTCGTTGTCTTTTGGCTGCACTCATCCATCATAGTATAGTTGTTGTTCAATCACAAGGGTGGTTTTCGCAATTTAGTGGGTTTGTTCTGCAAATGTTGAATTTGTAAGTTATGTTATTAGTTGTATGGGAACTTTCATGAGGGGAAGTACTTTGGTTTGTGGTTAGCTTTAGCTAATTTTATATACTTTTTTTTTCTCTCTTTCGTTCCAATTTGGGTGATGCCTTGAGGGAAAGAATTATTAAACTAATTTTAATAATATTCAAACTTTTGAATTGAAA

Coding sequence (CDS)

ATGGCGGACCACTCTGACAACGTGGAGTCGAAATCGGAGTCATTGATCGACAAGATAGTCGAGAAGATTCATGATCATGATTCTTCTTCGTCCGGATCAGACTCTGAAACCGAGGGATCCTTGAAGGAGAAGGTCTTCCGCCTTTTTGGAAGGGAGAGGCCAGTTCATCATGTCTTCGGCGGTGGAAAACCTGCTGATTTATTCTTGTGGAGGAATAAGAAGATCTCAGCTGGTGTCCTTACTGGAGCCACTGTTATCTGGGTTTTCTTTGAACTGCTCGAGTGCCATCTGCTTACGTTTGTTTCTCGTTCTCTGATCCTCTCTCTTGGGCTCTTGTTTCTTTGGTCCAATGCCCACACATTTATCTACAATTACAGGAGTCCACCCCGAATCCCAGAAGTTCAACTTCCTGCGGACCCCTTCCTGGAGTTTGCTTCAACTTTGAGGGCGGAAATTAATTGTGGTTTTGCATTATTGCGGGACATTGCTACCGGAAGAGATTTGAAGAAATTCATCACTGCAATTGCTGGGTTGTGGGTCCTGTCGATCGTGGGAAGCTGGTGCAATTTCTTGACGTTGTTCTACATAAACTTTGTTTTGTTGCACACTGTTCCTGTTCTGTATGAGAAGTATGAAGATAAGATCGACCCATTAGCGGAAAAAGCATTAATTGAGTTCAAAAAGCACTACGCCGTGTTTGAAGAAAAGGTTTTGAGCAAGATCCCAAAAGGGCCATTGAAAGGTAAGAAGGCTTAA

Protein sequence

MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSKIPKGPLKGKKA
Homology
BLAST of Carg20863 vs. NCBI nr
Match: KAG7028577.1 (Reticulon-like protein B5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 499.6 bits (1285), Expect = 1.6e-137
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60
           MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG
Sbjct: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60

Query: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120
           GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT
Sbjct: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120

Query: 121 FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180
           FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV
Sbjct: 121 FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180

Query: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240
           LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK
Sbjct: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240

Query: 241 IPKGPLKGKKA 252
           IPKGPLKGKKA
Sbjct: 241 IPKGPLKGKKA 251

BLAST of Carg20863 vs. NCBI nr
Match: XP_022933381.1 (reticulon-like protein B1 [Cucurbita moschata])

HSP 1 Score: 488.4 bits (1256), Expect = 3.7e-134
Identity = 248/251 (98.80%), Postives = 249/251 (99.20%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60
           MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG
Sbjct: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60

Query: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120
           GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT
Sbjct: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120

Query: 121 FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180
           FI  Y+SPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV
Sbjct: 121 FI--YKSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180

Query: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240
           LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK
Sbjct: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240

Query: 241 IPKGPLKGKKA 252
           IPKGPLKGKKA
Sbjct: 241 IPKGPLKGKKA 249

BLAST of Carg20863 vs. NCBI nr
Match: XP_023540787.1 (reticulon-like protein B1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.1 bits (1250), Expect = 1.8e-133
Identity = 247/251 (98.41%), Postives = 248/251 (98.80%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60
           MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG
Sbjct: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60

Query: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120
           GGKPADL LWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT
Sbjct: 61  GGKPADLLLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120

Query: 121 FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180
           FI  Y+SPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV
Sbjct: 121 FI--YKSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180

Query: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240
           LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK
Sbjct: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240

Query: 241 IPKGPLKGKKA 252
           IPKGPLKGKKA
Sbjct: 241 IPKGPLKGKKA 249

BLAST of Carg20863 vs. NCBI nr
Match: XP_022974656.1 (reticulon-like protein B1 [Cucurbita maxima] >XP_022975040.1 reticulon-like protein B1 [Cucurbita maxima])

HSP 1 Score: 483.4 bits (1243), Expect = 1.2e-132
Identity = 245/251 (97.61%), Postives = 248/251 (98.80%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60
           MA+HSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG
Sbjct: 1   MAEHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60

Query: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120
           GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLF+WSNAHT
Sbjct: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFVWSNAHT 120

Query: 121 FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180
           FI  Y+SPPRIPEVQLPADPFLE ASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV
Sbjct: 121 FI--YKSPPRIPEVQLPADPFLELASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180

Query: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240
           LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK
Sbjct: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240

Query: 241 IPKGPLKGKKA 252
           IPKGPLKGKKA
Sbjct: 241 IPKGPLKGKKA 249

BLAST of Carg20863 vs. NCBI nr
Match: KAG6597115.1 (Reticulon-like protein B5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 481.1 bits (1237), Expect = 5.8e-132
Identity = 244/247 (98.79%), Postives = 245/247 (99.19%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60
           MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG
Sbjct: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60

Query: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120
           GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT
Sbjct: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120

Query: 121 FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180
           FI  Y+SPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV
Sbjct: 121 FI--YKSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180

Query: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240
           LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK
Sbjct: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240

Query: 241 IPKGPLK 248
           IPKGPLK
Sbjct: 241 IPKGPLK 245

BLAST of Carg20863 vs. ExPASy Swiss-Prot
Match: Q9SUR3 (Reticulon-like protein B1 OS=Arabidopsis thaliana OX=3702 GN=RTNLB1 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 4.1e-80
Identity = 160/252 (63.49%), Postives = 188/252 (74.60%), Query Frame = 0

Query: 13  ESLIDKIVEKIH---DHDSSSSGSDSETE-----------GSLKEKVFRLFGRERPVHHV 72
           ESL+DKI EKIH   D  SSSS SD E E            S+K KV+RLFGRE+PVH V
Sbjct: 24  ESLMDKISEKIHHGGDSSSSSSSSDDEDEKKKTKKPSSPSSSMKSKVYRLFGREQPVHKV 83

Query: 73  FGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNA 132
            GGGKPAD+F+W+NKK+S GVL GAT  WV FEL+E HLLT +   +I+ L +LFLWSNA
Sbjct: 84  LGGGKPADIFMWKNKKMSGGVLGGATAAWVVFELMEYHLLTLLCHVMIVVLAVLFLWSNA 143

Query: 133 HTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGL 192
             FI   +SPP+IPEV +P +P L+ AS LR EIN GF+ LR+IA+GRDLKKF+ AIAGL
Sbjct: 144 TMFI--NKSPPKIPEVHIPEEPILQLASGLRIEINRGFSSLREIASGRDLKKFLIAIAGL 203

Query: 193 WVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVL 251
           WVLSI+G   NFLTL YI  VLL TVP+ Y+KYEDK+DPL EKA+IE KK YAV +EKVL
Sbjct: 204 WVLSILGGCFNFLTLAYIALVLLFTVPLAYDKYEDKVDPLGEKAMIELKKQYAVLDEKVL 263

BLAST of Carg20863 vs. ExPASy Swiss-Prot
Match: O82352 (Reticulon-like protein B5 OS=Arabidopsis thaliana OX=3702 GN=RTNLB5 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 7.3e-77
Identity = 150/254 (59.06%), Postives = 190/254 (74.80%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETE-----GSLKEKVFRLFGRERPV 60
           MA+  +      ESL++KI EKIH HDSSSS S+SE E      ++K K++R+FGRE+PV
Sbjct: 1   MAEEIEKSVPTEESLMEKISEKIHHHDSSSS-SESEYEKPDSPSAVKAKIYRMFGREKPV 60

Query: 61  HHVFGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLW 120
           H V GGGKPAD+FLWR+KK+S  VL  AT IWV FEL+E HLL+ +    IL+LG LFLW
Sbjct: 61  HKVLGGGKPADVFLWRDKKLSGAVLGVATAIWVLFELVEYHLLSLLCHISILALGGLFLW 120

Query: 121 SNAHTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAI 180
           SNAHT I   ++ P+IPE+ +P + FL  AS+LR E+N  F +LR IA GRDLKKF+  +
Sbjct: 121 SNAHTLI--NKTSPQIPEIHVPEEAFLVVASSLRNELNQAFVILRSIALGRDLKKFLMVV 180

Query: 181 AGLWVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEE 240
            GLW++S+VG+W NFLTL YI FV+LHTVP+LYEK+EDK+DPLAEKA+ E +K Y VF+E
Sbjct: 181 VGLWIISVVGNWFNFLTLVYICFVILHTVPMLYEKHEDKVDPLAEKAMKELQKQYVVFDE 240

Query: 241 KVLSKIPKGPLKGK 250
           KVLSKIP   LK K
Sbjct: 241 KVLSKIPIASLKAK 251

BLAST of Carg20863 vs. ExPASy Swiss-Prot
Match: Q6DBN4 (Reticulon-like protein B6 OS=Arabidopsis thaliana OX=3702 GN=RTNLB6 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 7.3e-77
Identity = 150/254 (59.06%), Postives = 187/254 (73.62%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETE-----GSLKEKVFRLFGRERPV 60
           MA+  +    K ESL++KI +KIHDHDSSSS SDSE E      +LK K++RLFGRE+PV
Sbjct: 1   MAEELEKSMHKEESLMEKIADKIHDHDSSSS-SDSEHEKPESPSALKAKIYRLFGREKPV 60

Query: 61  HHVFGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLW 120
           H V GGG PAD+FLWR+KK+SA VL  AT IWV FEL+E H L+ V   LI +L  LFL 
Sbjct: 61  HKVLGGGLPADVFLWRDKKLSASVLGVATAIWVLFELVEYHFLSLVCHILIFALAALFLL 120

Query: 121 SNAHTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAI 180
           SNAH F+   ++PP+IPE+ +  + FL   S LR E+N  F +LR IA GRDLKKF+  +
Sbjct: 121 SNAHAFM--NKTPPKIPEIHIKEEHFLMIVSALRNELNQAFVILRSIALGRDLKKFLMVV 180

Query: 181 AGLWVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEE 240
            GLW++S+VG+W NFLTL YI FV+LHTVP+LYEK+EDK+DP+AEK L E KKHY VF+E
Sbjct: 181 FGLWIISVVGNWFNFLTLVYICFVVLHTVPMLYEKHEDKVDPVAEKTLKELKKHYMVFDE 240

Query: 241 KVLSKIPKGPLKGK 250
           KVLSK+P   LK K
Sbjct: 241 KVLSKLPVASLKAK 251

BLAST of Carg20863 vs. ExPASy Swiss-Prot
Match: Q9SUT9 (Reticulon-like protein B2 OS=Arabidopsis thaliana OX=3702 GN=RTNLB2 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 3.6e-76
Identity = 148/248 (59.68%), Postives = 184/248 (74.19%), Query Frame = 0

Query: 13  ESLIDKIVEKIHDHDSSSSGSDSETEG----------SLKEKVFRLFGRERPVHHVFGGG 72
           ESL++K+ EKIH    SSS S S+ E           SLK KV+RLFGRERPVH V GGG
Sbjct: 24  ESLMEKLSEKIHHKGDSSSSSSSDDENEKKSSSSSPKSLKSKVYRLFGRERPVHKVLGGG 83

Query: 73  KPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHTFI 132
           KPAD+F+W++KK+S GV  GATV WV FEL+E HLLT +   +I++L +LFLWSNA  FI
Sbjct: 84  KPADIFMWKDKKMSGGVFGGATVAWVLFELMEYHLLTLLCHVMIVALAVLFLWSNATMFI 143

Query: 133 YNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWVLS 192
             ++SPP+IPEV +P +P L+ AS LR EIN G + LR+IA+GRD+KKF++AIAGLWVLS
Sbjct: 144 --HKSPPKIPEVHIPEEPLLQLASGLRIEINRGISSLREIASGRDIKKFLSAIAGLWVLS 203

Query: 193 IVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSKIP 251
           I+G   +FLTL YI  VLL TVP+ Y+KYEDK+D   EKA+ E KK YAV + KV SKIP
Sbjct: 204 ILGGCYSFLTLAYIALVLLFTVPLFYDKYEDKVDSYGEKAMAELKKQYAVLDAKVFSKIP 263

BLAST of Carg20863 vs. ExPASy Swiss-Prot
Match: Q9FFS0 (Reticulon-like protein B4 OS=Arabidopsis thaliana OX=3702 GN=RTNLB4 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.8e-75
Identity = 152/250 (60.80%), Postives = 178/250 (71.20%), Query Frame = 0

Query: 13  ESLIDKIVEKIHDHDSSSSGSDSE--------TEGSLKEKVFRLFGRERPVHHVFGGGKP 72
           ES+++KIVEKIH H  SSS SDS+        +  S K K++RLFGRE+PVH V GGGKP
Sbjct: 9   ESILEKIVEKIHGHGDSSSLSDSDDDKKSTSSSSSSFKSKIYRLFGREKPVHKVLGGGKP 68

Query: 73  ADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHTFIYN 132
           AD+FLWRNKK+S GVL   T  WV FEL E HLL F+    I +L  LFLWSNA TFI  
Sbjct: 69  ADIFLWRNKKVSGGVLGAVTASWVLFELFEYHLLAFLCHFAIFALAALFLWSNACTFI-- 128

Query: 133 YRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWVLSIV 192
           ++S P IPEV +P DP L+  S LR EIN G  LLR+IA+G+D+KKFI  IAGLWVLSI+
Sbjct: 129 HKSTPHIPEVHIPEDPILQLVSGLRIEINRGLTLLRNIASGKDVKKFILVIAGLWVLSII 188

Query: 193 GSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVL----SK 251
           GS  NFLTLFY   VLL T+PVLYEKYEDK+D   EKA+ E KK YAV +EKVL    SK
Sbjct: 189 GSCYNFLTLFYTATVLLFTIPVLYEKYEDKVDAYGEKAMREIKKQYAVLDEKVLRKVISK 248

BLAST of Carg20863 vs. ExPASy TrEMBL
Match: A0A6J1EYW5 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111440810 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.8e-134
Identity = 248/251 (98.80%), Postives = 249/251 (99.20%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60
           MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG
Sbjct: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60

Query: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120
           GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT
Sbjct: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120

Query: 121 FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180
           FI  Y+SPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV
Sbjct: 121 FI--YKSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180

Query: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240
           LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK
Sbjct: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240

Query: 241 IPKGPLKGKKA 252
           IPKGPLKGKKA
Sbjct: 241 IPKGPLKGKKA 249

BLAST of Carg20863 vs. ExPASy TrEMBL
Match: A0A6J1IFJ8 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111473997 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 5.7e-133
Identity = 245/251 (97.61%), Postives = 248/251 (98.80%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60
           MA+HSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG
Sbjct: 1   MAEHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETEGSLKEKVFRLFGRERPVHHVFG 60

Query: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHT 120
           GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLF+WSNAHT
Sbjct: 61  GGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFVWSNAHT 120

Query: 121 FIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180
           FI  Y+SPPRIPEVQLPADPFLE ASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV
Sbjct: 121 FI--YKSPPRIPEVQLPADPFLELASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWV 180

Query: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240
           LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK
Sbjct: 181 LSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSK 240

Query: 241 IPKGPLKGKKA 252
           IPKGPLKGKKA
Sbjct: 241 IPKGPLKGKKA 249

BLAST of Carg20863 vs. ExPASy TrEMBL
Match: A0A6J1E9F7 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111432044 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.4e-125
Identity = 237/257 (92.22%), Postives = 243/257 (94.55%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHD-SSSSGSDSETEG-----SLKEKVFRLFGRERP 60
           M+DHS+N E KSESLIDKIVEKIHDHD SSSSGSDSETEG     SLKEKVFRLFGRERP
Sbjct: 1   MSDHSENAEPKSESLIDKIVEKIHDHDSSSSSGSDSETEGSASSSSLKEKVFRLFGRERP 60

Query: 61  VHHVFGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFL 120
           VHHVFGGGKPADLFLWRNKKISAGVL+GATVIWVFFELLECHLLTFVSRSLIL+LGLLFL
Sbjct: 61  VHHVFGGGKPADLFLWRNKKISAGVLSGATVIWVFFELLECHLLTFVSRSLILALGLLFL 120

Query: 121 WSNAHTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITA 180
           WSNAHTFI  Y+S PRIP VQLPADPFLEFASTLRAEINCG ALLRDIATGRDLKKFITA
Sbjct: 121 WSNAHTFI--YKSAPRIPVVQLPADPFLEFASTLRAEINCGIALLRDIATGRDLKKFITA 180

Query: 181 IAGLWVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFE 240
           IA LWVLSIVGSWCNFLTLFYINFVLLHTVPV+YEKYEDKIDPLAEKALIEFKKHYAVFE
Sbjct: 181 IAALWVLSIVGSWCNFLTLFYINFVLLHTVPVVYEKYEDKIDPLAEKALIEFKKHYAVFE 240

Query: 241 EKVLSKIPKGPLKGKKA 252
           EKVLSKIPKGPLKGKKA
Sbjct: 241 EKVLSKIPKGPLKGKKA 255

BLAST of Carg20863 vs. ExPASy TrEMBL
Match: A0A6J1ISM8 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111479592 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 8.2e-124
Identity = 235/257 (91.44%), Postives = 241/257 (93.77%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHD-SSSSGSDSETEG-----SLKEKVFRLFGRERP 60
           M+DHS+N E KSESLIDKIVEKIHDHD SSSSGSDSETEG     SLKEKVFRLFGRERP
Sbjct: 1   MSDHSENAEPKSESLIDKIVEKIHDHDSSSSSGSDSETEGSASSSSLKEKVFRLFGRERP 60

Query: 61  VHHVFGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFL 120
           VHHVFGGGKPADLFLWRNKKISAGVL+GAT IWVFFELLECHLLTFVSRSLIL+LGLLFL
Sbjct: 61  VHHVFGGGKPADLFLWRNKKISAGVLSGATAIWVFFELLECHLLTFVSRSLILALGLLFL 120

Query: 121 WSNAHTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITA 180
           WSNAHTFI  Y+S PRIP VQLPADPFLEFASTLRAEINCG ALLRDIATGRDLKKFITA
Sbjct: 121 WSNAHTFI--YKSAPRIPVVQLPADPFLEFASTLRAEINCGIALLRDIATGRDLKKFITA 180

Query: 181 IAGLWVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFE 240
           IA LWVLSIVGSWCNFLTLFYINFVLLHTVPV+YEKYEDKIDPLAEKAL EFKKHYAVFE
Sbjct: 181 IAALWVLSIVGSWCNFLTLFYINFVLLHTVPVVYEKYEDKIDPLAEKALREFKKHYAVFE 240

Query: 241 EKVLSKIPKGPLKGKKA 252
           EKVLSKIPKGPLKGKKA
Sbjct: 241 EKVLSKIPKGPLKGKKA 255

BLAST of Carg20863 vs. ExPASy TrEMBL
Match: A0A5A7U3V2 (Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001560 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.1e-123
Identity = 229/257 (89.11%), Postives = 243/257 (94.55%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHD-SSSSGSDSETEGS-----LKEKVFRLFGRERP 60
           M+DH++N E KSESLIDKIVEKIHDHD SSSSGSD+ETEGS     LKEKVFR+FGRERP
Sbjct: 1   MSDHAENTEPKSESLIDKIVEKIHDHDSSSSSGSDAETEGSASSSTLKEKVFRIFGRERP 60

Query: 61  VHHVFGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFL 120
           VHHV GGGKPADLFLWRNKKISAGVL+GAT+IWVFFELLECHLLTF+SRSLIL+LGLLFL
Sbjct: 61  VHHVLGGGKPADLFLWRNKKISAGVLSGATLIWVFFELLECHLLTFISRSLILALGLLFL 120

Query: 121 WSNAHTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITA 180
           WSNAHTFI  Y+SPPRIP VQLPADPF+E ASTLRAEINCGFALLRDIATGRDLKKF+TA
Sbjct: 121 WSNAHTFI--YKSPPRIPVVQLPADPFMELASTLRAEINCGFALLRDIATGRDLKKFLTA 180

Query: 181 IAGLWVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFE 240
           IA LWVLSIVGSWCNFLTLFYINFVLLHTVPV+YEKYEDKIDPLAEKA+IEFKKHYAVFE
Sbjct: 181 IAALWVLSIVGSWCNFLTLFYINFVLLHTVPVVYEKYEDKIDPLAEKAVIEFKKHYAVFE 240

Query: 241 EKVLSKIPKGPLKGKKA 252
           EKVLSKIPKGPLKGKKA
Sbjct: 241 EKVLSKIPKGPLKGKKA 255

BLAST of Carg20863 vs. TAIR 10
Match: AT4G23630.1 (VIRB2-interacting protein 1 )

HSP 1 Score: 299.3 bits (765), Expect = 2.9e-81
Identity = 160/252 (63.49%), Postives = 188/252 (74.60%), Query Frame = 0

Query: 13  ESLIDKIVEKIH---DHDSSSSGSDSETE-----------GSLKEKVFRLFGRERPVHHV 72
           ESL+DKI EKIH   D  SSSS SD E E            S+K KV+RLFGRE+PVH V
Sbjct: 24  ESLMDKISEKIHHGGDSSSSSSSSDDEDEKKKTKKPSSPSSSMKSKVYRLFGREQPVHKV 83

Query: 73  FGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNA 132
            GGGKPAD+F+W+NKK+S GVL GAT  WV FEL+E HLLT +   +I+ L +LFLWSNA
Sbjct: 84  LGGGKPADIFMWKNKKMSGGVLGGATAAWVVFELMEYHLLTLLCHVMIVVLAVLFLWSNA 143

Query: 133 HTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGL 192
             FI   +SPP+IPEV +P +P L+ AS LR EIN GF+ LR+IA+GRDLKKF+ AIAGL
Sbjct: 144 TMFI--NKSPPKIPEVHIPEEPILQLASGLRIEINRGFSSLREIASGRDLKKFLIAIAGL 203

Query: 193 WVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVL 251
           WVLSI+G   NFLTL YI  VLL TVP+ Y+KYEDK+DPL EKA+IE KK YAV +EKVL
Sbjct: 204 WVLSILGGCFNFLTLAYIALVLLFTVPLAYDKYEDKVDPLGEKAMIELKKQYAVLDEKVL 263

BLAST of Carg20863 vs. TAIR 10
Match: AT2G46170.1 (Reticulon family protein )

HSP 1 Score: 288.5 bits (737), Expect = 5.2e-78
Identity = 150/254 (59.06%), Postives = 190/254 (74.80%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETE-----GSLKEKVFRLFGRERPV 60
           MA+  +      ESL++KI EKIH HDSSSS S+SE E      ++K K++R+FGRE+PV
Sbjct: 1   MAEEIEKSVPTEESLMEKISEKIHHHDSSSS-SESEYEKPDSPSAVKAKIYRMFGREKPV 60

Query: 61  HHVFGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLW 120
           H V GGGKPAD+FLWR+KK+S  VL  AT IWV FEL+E HLL+ +    IL+LG LFLW
Sbjct: 61  HKVLGGGKPADVFLWRDKKLSGAVLGVATAIWVLFELVEYHLLSLLCHISILALGGLFLW 120

Query: 121 SNAHTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAI 180
           SNAHT I   ++ P+IPE+ +P + FL  AS+LR E+N  F +LR IA GRDLKKF+  +
Sbjct: 121 SNAHTLI--NKTSPQIPEIHVPEEAFLVVASSLRNELNQAFVILRSIALGRDLKKFLMVV 180

Query: 181 AGLWVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEE 240
            GLW++S+VG+W NFLTL YI FV+LHTVP+LYEK+EDK+DPLAEKA+ E +K Y VF+E
Sbjct: 181 VGLWIISVVGNWFNFLTLVYICFVILHTVPMLYEKHEDKVDPLAEKAMKELQKQYVVFDE 240

Query: 241 KVLSKIPKGPLKGK 250
           KVLSKIP   LK K
Sbjct: 241 KVLSKIPIASLKAK 251

BLAST of Carg20863 vs. TAIR 10
Match: AT3G61560.1 (Reticulon family protein )

HSP 1 Score: 288.5 bits (737), Expect = 5.2e-78
Identity = 150/254 (59.06%), Postives = 187/254 (73.62%), Query Frame = 0

Query: 1   MADHSDNVESKSESLIDKIVEKIHDHDSSSSGSDSETE-----GSLKEKVFRLFGRERPV 60
           MA+  +    K ESL++KI +KIHDHDSSSS SDSE E      +LK K++RLFGRE+PV
Sbjct: 1   MAEELEKSMHKEESLMEKIADKIHDHDSSSS-SDSEHEKPESPSALKAKIYRLFGREKPV 60

Query: 61  HHVFGGGKPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLW 120
           H V GGG PAD+FLWR+KK+SA VL  AT IWV FEL+E H L+ V   LI +L  LFL 
Sbjct: 61  HKVLGGGLPADVFLWRDKKLSASVLGVATAIWVLFELVEYHFLSLVCHILIFALAALFLL 120

Query: 121 SNAHTFIYNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAI 180
           SNAH F+   ++PP+IPE+ +  + FL   S LR E+N  F +LR IA GRDLKKF+  +
Sbjct: 121 SNAHAFM--NKTPPKIPEIHIKEEHFLMIVSALRNELNQAFVILRSIALGRDLKKFLMVV 180

Query: 181 AGLWVLSIVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEE 240
            GLW++S+VG+W NFLTL YI FV+LHTVP+LYEK+EDK+DP+AEK L E KKHY VF+E
Sbjct: 181 FGLWIISVVGNWFNFLTLVYICFVVLHTVPMLYEKHEDKVDPVAEKTLKELKKHYMVFDE 240

Query: 241 KVLSKIPKGPLKGK 250
           KVLSK+P   LK K
Sbjct: 241 KVLSKLPVASLKAK 251

BLAST of Carg20863 vs. TAIR 10
Match: AT4G11220.1 (VIRB2-interacting protein 2 )

HSP 1 Score: 286.2 bits (731), Expect = 2.6e-77
Identity = 148/248 (59.68%), Postives = 184/248 (74.19%), Query Frame = 0

Query: 13  ESLIDKIVEKIHDHDSSSSGSDSETEG----------SLKEKVFRLFGRERPVHHVFGGG 72
           ESL++K+ EKIH    SSS S S+ E           SLK KV+RLFGRERPVH V GGG
Sbjct: 24  ESLMEKLSEKIHHKGDSSSSSSSDDENEKKSSSSSPKSLKSKVYRLFGRERPVHKVLGGG 83

Query: 73  KPADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHTFI 132
           KPAD+F+W++KK+S GV  GATV WV FEL+E HLLT +   +I++L +LFLWSNA  FI
Sbjct: 84  KPADIFMWKDKKMSGGVFGGATVAWVLFELMEYHLLTLLCHVMIVALAVLFLWSNATMFI 143

Query: 133 YNYRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWVLS 192
             ++SPP+IPEV +P +P L+ AS LR EIN G + LR+IA+GRD+KKF++AIAGLWVLS
Sbjct: 144 --HKSPPKIPEVHIPEEPLLQLASGLRIEINRGISSLREIASGRDIKKFLSAIAGLWVLS 203

Query: 193 IVGSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVLSKIP 251
           I+G   +FLTL YI  VLL TVP+ Y+KYEDK+D   EKA+ E KK YAV + KV SKIP
Sbjct: 204 ILGGCYSFLTLAYIALVLLFTVPLFYDKYEDKVDSYGEKAMAELKKQYAVLDAKVFSKIP 263

BLAST of Carg20863 vs. TAIR 10
Match: AT5G41600.1 (VIRB2-interacting protein 3 )

HSP 1 Score: 283.9 bits (725), Expect = 1.3e-76
Identity = 152/250 (60.80%), Postives = 178/250 (71.20%), Query Frame = 0

Query: 13  ESLIDKIVEKIHDHDSSSSGSDSE--------TEGSLKEKVFRLFGRERPVHHVFGGGKP 72
           ES+++KIVEKIH H  SSS SDS+        +  S K K++RLFGRE+PVH V GGGKP
Sbjct: 9   ESILEKIVEKIHGHGDSSSLSDSDDDKKSTSSSSSSFKSKIYRLFGREKPVHKVLGGGKP 68

Query: 73  ADLFLWRNKKISAGVLTGATVIWVFFELLECHLLTFVSRSLILSLGLLFLWSNAHTFIYN 132
           AD+FLWRNKK+S GVL   T  WV FEL E HLL F+    I +L  LFLWSNA TFI  
Sbjct: 69  ADIFLWRNKKVSGGVLGAVTASWVLFELFEYHLLAFLCHFAIFALAALFLWSNACTFI-- 128

Query: 133 YRSPPRIPEVQLPADPFLEFASTLRAEINCGFALLRDIATGRDLKKFITAIAGLWVLSIV 192
           ++S P IPEV +P DP L+  S LR EIN G  LLR+IA+G+D+KKFI  IAGLWVLSI+
Sbjct: 129 HKSTPHIPEVHIPEDPILQLVSGLRIEINRGLTLLRNIASGKDVKKFILVIAGLWVLSII 188

Query: 193 GSWCNFLTLFYINFVLLHTVPVLYEKYEDKIDPLAEKALIEFKKHYAVFEEKVL----SK 251
           GS  NFLTLFY   VLL T+PVLYEKYEDK+D   EKA+ E KK YAV +EKVL    SK
Sbjct: 189 GSCYNFLTLFYTATVLLFTIPVLYEKYEDKVDAYGEKAMREIKKQYAVLDEKVLRKVISK 248

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028577.11.6e-137100.00Reticulon-like protein B5 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022933381.13.7e-13498.80reticulon-like protein B1 [Cucurbita moschata][more]
XP_023540787.11.8e-13398.41reticulon-like protein B1 [Cucurbita pepo subsp. pepo][more]
XP_022974656.11.2e-13297.61reticulon-like protein B1 [Cucurbita maxima] >XP_022975040.1 reticulon-like prot... [more]
KAG6597115.15.8e-13298.79Reticulon-like protein B5, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9SUR34.1e-8063.49Reticulon-like protein B1 OS=Arabidopsis thaliana OX=3702 GN=RTNLB1 PE=1 SV=1[more]
O823527.3e-7759.06Reticulon-like protein B5 OS=Arabidopsis thaliana OX=3702 GN=RTNLB5 PE=1 SV=1[more]
Q6DBN47.3e-7759.06Reticulon-like protein B6 OS=Arabidopsis thaliana OX=3702 GN=RTNLB6 PE=1 SV=1[more]
Q9SUT93.6e-7659.68Reticulon-like protein B2 OS=Arabidopsis thaliana OX=3702 GN=RTNLB2 PE=1 SV=1[more]
Q9FFS01.8e-7560.80Reticulon-like protein B4 OS=Arabidopsis thaliana OX=3702 GN=RTNLB4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EYW51.8e-13498.80Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111440810 PE=4 SV=1[more]
A0A6J1IFJ85.7e-13397.61Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111473997 PE=4 SV=1[more]
A0A6J1E9F74.4e-12592.22Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111432044 PE=4 SV=1[more]
A0A6J1ISM88.2e-12491.44Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111479592 PE=4 SV=1[more]
A0A5A7U3V24.1e-12389.11Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
Match NameE-valueIdentityDescription
AT4G23630.12.9e-8163.49VIRB2-interacting protein 1 [more]
AT2G46170.15.2e-7859.06Reticulon family protein [more]
AT3G61560.15.2e-7859.06Reticulon family protein [more]
AT4G11220.12.6e-7759.68VIRB2-interacting protein 2 [more]
AT5G41600.11.3e-7660.80VIRB2-interacting protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003388ReticulonPFAMPF02453Reticuloncoord: 65..222
e-value: 5.9E-52
score: 175.8
IPR003388ReticulonPROSITEPS50845RETICULONcoord: 64..251
score: 41.948303
IPR045064Reticulon-like protein, plantPANTHERPTHR10994RETICULONcoord: 1..250
NoneNo IPR availablePANTHERPTHR10994:SF159RETICULON-LIKE PROTEINcoord: 1..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20863-RACarg20863-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009617 response to bacterium
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane