Carg20576 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20576
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionACT domain containing protein
LocationCarg_Chr12: 586406 .. 588864 (-)
RNA-Seq ExpressionCarg20576
SyntenyCarg20576
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGAGATCTCCCTTGGCTTGTGTATATAATCTTCTCCTTCGCTTTCTCGTTGAATACAAAGCCTTGTTTGCGGCGTTTCAGAACAAACTGAGAAGAAACCTTCAACATTCTGAACAGATTACAAAATCCTCAGAGATCGAAAATGGGTATGTTCTCTTTGGTTTTCTCTCTGTCTCTCTCCTTCCCTTTCCTCTGTTCTTCTCTTACTTGGTTTTTAGTCTCTTCTCTGGTTTTCTTGTTTGGGATTCTTCTCTTTTCCTCCTTATCATTTTCAGATCTCTCTCTCTCTCTCTCTCCCTCTCTCCCTCTAGATGGTCTGGTCATGGCTTTTGATACTTGTTTTCTGATTGGCTTTACAAGATTACCATGGCGTGTCTTATTTGTTGGTCTATTTACCCCTTTTCTCTTATTCCAGCAATCGACATGAACTTTTCCCATGATATTGATGATGAATATGTGAAGCTGATTAGAAGGATGAATCCAACCAGGTACATAATTTGCTCCCTTTCTCTGAAGAAAAAAAAACACAACTTTCTTCTGTACAAAAATTAAGCTTTTAAGTTATGTCTTTTTCCAGGGTTGTTATTGACAATGAAGCCTGTAAGGATGCTACTGTTATAAGGGTATATGATTTTGAACTCTGTTTCTTATTTGCTGAAGTCTTCCATTTGGGTTAAAATTATGCTCATTTCAACCATTTTGTTGCAGGTAGATAGCGCCAATAAACATGGGATTCTTCTTGAAGTCGTTCAAGTTCTTACTGATCTTAATCTCATCGTCACCAAAGCCTATATTTCTTGTGATGGTTGTTGGTTCATGGATGGTAAGTTTCTCTTTCCCATTTTTCTCTCTTTTATTGGTTCTCTCCCGATCAGGATTTTCATGTGGTTTTGGCTTCTTGGACAGTTTTTAATGTCACTGATCAAGATGGAAACAAAGTTACAGATGAAGGGGTTTTAGACTATATCAAAAGGGTATGTGCTATGTGCCTGTTTCTATCTTTCTGTTGTATTTTTTCTTTCTTTCTTTCTTTCATGTGTTTCATTCAATTAATTCAACGCGGTTATGTTTTTTGGTTTTCAGTCGCTTGAGTCGGATTCTTGTTTTGCATCTTCCATGAGATCTGTGGGTGTAAAACCATCGGTTGATTACACAGCAATTGAGCTTATTGGAAATGATAGACAAGGATTACTTTCTGAACTCAGCGCGGTCCTCACTCACCTCAAATGCAACGTTGTGCACGCCGAGGTGTGGACTCATAACACACGGGCGGCCGCCGTGATGCATGTCACCGACGACGAAACTGGTTCTGCAATTACTGATCTTGAGAGGCTATCTAGGATCAAGGGATTACTCTGTAATGTACTAAGGGGAAGCAACAATAGGACCAATGGAGCTAAGACTGTTGTGTCTCAAGGAATCACTCACATTGACAGAAGGCTTCATCAGATGATGTTTGCTGATAGGGATTATGAACTTGTTGGTGAAGATTTAATGGACGAGAAGCAAAGGCCTAATGTCAAGGTTGTGAATTGGTGTGACATAGACTATTCTGTCATTACAATTCGGAGCAAAGATCGCCCGAAACTTCTTTTCGACACGATTTGCACGTTGACAGATATGCAATATGTGGTCTTCCATGCCAATGTAGTTGCTGAAGGGGCAGAAGCCTATCAGGTAAGATTGCAATCACTTGCATCTTTAGCAACTTCGAGACAATGTGGACTTGTTCTTTAGGCTTATGATCTCTATTGTGAATTTGTAGGAATATTACATTAGACATATAGATGGATCTCCTGTTAAATCTGATGCAGAGAAACAAAGGGTAATTCAATGTCTCGAAGCTGCTATAAGGAGAAGAGTGTCTGAGGTGCGTGAAGAACACTACCATATCACCGAAACTAACGACTATTTTTCGCGGTCGTTCGAGTTTTTTCGCTTTGTTCGAATTGTTGATTCATTGTTCTATCTTGTTTTTCAGGGCTTGAAGCTTGAGTTATGCACAACAGATAGAGTAGGCCTTCTCTCTGATGTCACCCGCATCTTCCGAGAGAACAGCCTGACTGTCACAAGAGCAGAAGTATCAACTAAAGGCGGTAAGGCGATTAATACGTTCTACGTTCGTGATGCGTCGGGGTATCCAGTCGATTCGAAGACGATAGATTCAATTCGAGATTTGATCGGGCAAACAATACTGAAAGTGAAAGGCAGTCCAAAAGAACAAAAACAAGGTTCTTCTCAGGATTCACCAACTAGATTCTTATTTGGTGGTCTTTTTAGATCTAGATCTTTTGTAAATTTTGGGTTAATAAGGTCATAATCATGAGGAGATCTACTGCTACTGAATTGATTTAAGCCCATCATGTAAATGCACCATAAATACTACATAGGAACCAAAACATGGAGCACCATCAGCTCCTTCCTTCTTGTACATAACTCAGAAGAATTAGGCTCTTCTC

mRNA sequence

CCGAGATCTCCCTTGGCTTGTGTATATAATCTTCTCCTTCGCTTTCTCGTTGAATACAAAGCCTTGTTTGCGGCGTTTCAGAACAAACTGAGAAGAAACCTTCAACATTCTGAACAGATTACAAAATCCTCAGAGATCGAAAATGGCAATCGACATGAACTTTTCCCATGATATTGATGATGAATATGTGAAGCTGATTAGAAGGATGAATCCAACCAGGGTTGTTATTGACAATGAAGCCTGTAAGGATGCTACTGTTATAAGGGTAGATAGCGCCAATAAACATGGGATTCTTCTTGAAGTCGTTCAAGTTCTTACTGATCTTAATCTCATCGTCACCAAAGCCTATATTTCTTGTGATGGTTGTTGGTTCATGGATGTTTTTAATGTCACTGATCAAGATGGAAACAAAGTTACAGATGAAGGGGTTTTAGACTATATCAAAAGGTCGCTTGAGTCGGATTCTTGTTTTGCATCTTCCATGAGATCTGTGGGTGTAAAACCATCGGTTGATTACACAGCAATTGAGCTTATTGGAAATGATAGACAAGGATTACTTTCTGAACTCAGCGCGGTCCTCACTCACCTCAAATGCAACGTTGTGCACGCCGAGGTGTGGACTCATAACACACGGGCGGCCGCCGTGATGCATGTCACCGACGACGAAACTGGTTCTGCAATTACTGATCTTGAGAGGCTATCTAGGATCAAGGGATTACTCTGTAATGTACTAAGGGGAAGCAACAATAGGACCAATGGAGCTAAGACTGTTGTGTCTCAAGGAATCACTCACATTGACAGAAGGCTTCATCAGATGATGTTTGCTGATAGGGATTATGAACTTGTTGGTGAAGATTTAATGGACGAGAAGCAAAGGCCTAATGTCAAGGTTGTGAATTGGTGTGACATAGACTATTCTGTCATTACAATTCGGAGCAAAGATCGCCCGAAACTTCTTTTCGACACGATTTGCACGTTGACAGATATGCAATATGTGGTCTTCCATGCCAATGTAGTTGCTGAAGGGGCAGAAGCCTATCAGGAATATTACATTAGACATATAGATGGATCTCCTGTTAAATCTGATGCAGAGAAACAAAGGGTAATTCAATGTCTCGAAGCTGCTATAAGGAGAAGAGTGTCTGAGGGCTTGAAGCTTGAGTTATGCACAACAGATAGAGTAGGCCTTCTCTCTGATGTCACCCGCATCTTCCGAGAGAACAGCCTGACTGTCACAAGAGCAGAAGTATCAACTAAAGGCGGTAAGGCGATTAATACGTTCTACGTTCGTGATGCGTCGGGGTATCCAGTCGATTCGAAGACGATAGATTCAATTCGAGATTTGATCGGGCAAACAATACTGAAAGTGAAAGGCAGTCCAAAAGAACAAAAACAAGGTTCTTCTCAGGATTCACCAACTAGATTCTTATTTGGTGGTCTTTTTAGATCTAGATCTTTTGTAAATTTTGGGTTAATAAGGTCATAATCATGAGGAGATCTACTGCTACTGAATTGATTTAAGCCCATCATGTAAATGCACCATAAATACTACATAGGAACCAAAACATGGAGCACCATCAGCTCCTTCCTTCTTGTACATAACTCAGAAGAATTAGGCTCTTCTC

Coding sequence (CDS)

ATGGCAATCGACATGAACTTTTCCCATGATATTGATGATGAATATGTGAAGCTGATTAGAAGGATGAATCCAACCAGGGTTGTTATTGACAATGAAGCCTGTAAGGATGCTACTGTTATAAGGGTAGATAGCGCCAATAAACATGGGATTCTTCTTGAAGTCGTTCAAGTTCTTACTGATCTTAATCTCATCGTCACCAAAGCCTATATTTCTTGTGATGGTTGTTGGTTCATGGATGTTTTTAATGTCACTGATCAAGATGGAAACAAAGTTACAGATGAAGGGGTTTTAGACTATATCAAAAGGTCGCTTGAGTCGGATTCTTGTTTTGCATCTTCCATGAGATCTGTGGGTGTAAAACCATCGGTTGATTACACAGCAATTGAGCTTATTGGAAATGATAGACAAGGATTACTTTCTGAACTCAGCGCGGTCCTCACTCACCTCAAATGCAACGTTGTGCACGCCGAGGTGTGGACTCATAACACACGGGCGGCCGCCGTGATGCATGTCACCGACGACGAAACTGGTTCTGCAATTACTGATCTTGAGAGGCTATCTAGGATCAAGGGATTACTCTGTAATGTACTAAGGGGAAGCAACAATAGGACCAATGGAGCTAAGACTGTTGTGTCTCAAGGAATCACTCACATTGACAGAAGGCTTCATCAGATGATGTTTGCTGATAGGGATTATGAACTTGTTGGTGAAGATTTAATGGACGAGAAGCAAAGGCCTAATGTCAAGGTTGTGAATTGGTGTGACATAGACTATTCTGTCATTACAATTCGGAGCAAAGATCGCCCGAAACTTCTTTTCGACACGATTTGCACGTTGACAGATATGCAATATGTGGTCTTCCATGCCAATGTAGTTGCTGAAGGGGCAGAAGCCTATCAGGAATATTACATTAGACATATAGATGGATCTCCTGTTAAATCTGATGCAGAGAAACAAAGGGTAATTCAATGTCTCGAAGCTGCTATAAGGAGAAGAGTGTCTGAGGGCTTGAAGCTTGAGTTATGCACAACAGATAGAGTAGGCCTTCTCTCTGATGTCACCCGCATCTTCCGAGAGAACAGCCTGACTGTCACAAGAGCAGAAGTATCAACTAAAGGCGGTAAGGCGATTAATACGTTCTACGTTCGTGATGCGTCGGGGTATCCAGTCGATTCGAAGACGATAGATTCAATTCGAGATTTGATCGGGCAAACAATACTGAAAGTGAAAGGCAGTCCAAAAGAACAAAAACAAGGTTCTTCTCAGGATTCACCAACTAGATTCTTATTTGGTGGTCTTTTTAGATCTAGATCTTTTGTAAATTTTGGGTTAATAAGGTCATAA

Protein sequence

MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTDLNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVKPSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAITDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLMDEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQEYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFRENSLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGSSQDSPTRFLFGGLFRSRSFVNFGLIRS
Homology
BLAST of Carg20576 vs. NCBI nr
Match: KAG7020178.1 (ACT domain-containing protein ACR4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 885.6 bits (2287), Expect = 1.8e-253
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD
Sbjct: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM
Sbjct: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ
Sbjct: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420
           SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS
Sbjct: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420

Query: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 447

BLAST of Carg20576 vs. NCBI nr
Match: KAG6585256.1 (ACT domain-containing protein ACR4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 884.0 bits (2283), Expect = 5.3e-253
Identity = 446/447 (99.78%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD
Sbjct: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM
Sbjct: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGTEAYQ 300

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420
           SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS
Sbjct: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420

Query: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 447

BLAST of Carg20576 vs. NCBI nr
Match: XP_022951004.1 (ACT domain-containing protein ACR4-like [Cucurbita moschata] >XP_022951006.1 ACT domain-containing protein ACR4-like [Cucurbita moschata] >XP_022951007.1 ACT domain-containing protein ACR4-like [Cucurbita moschata] >XP_022951008.1 ACT domain-containing protein ACR4-like [Cucurbita moschata])

HSP 1 Score: 882.9 bits (2280), Expect = 1.2e-252
Identity = 445/447 (99.55%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD
Sbjct: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYEL+GEDLM
Sbjct: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELLGEDLM 240

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGTEAYQ 300

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420
           SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS
Sbjct: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420

Query: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 447

BLAST of Carg20576 vs. NCBI nr
Match: XP_023002814.1 (ACT domain-containing protein ACR4-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 875.2 bits (2260), Expect = 2.5e-250
Identity = 442/447 (98.88%), Postives = 445/447 (99.55%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD
Sbjct: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQG+THIDRRLHQMMFADRDYEL+GEDLM
Sbjct: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGVTHIDRRLHQMMFADRDYELLGEDLM 240

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGTEAYQ 300

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAEKQRVI+CLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 301 EYYIRHIDGSPVKSDAEKQRVIKCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420
           SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQG 
Sbjct: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQG- 420

Query: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 446

BLAST of Carg20576 vs. NCBI nr
Match: XP_023002812.1 (ACT domain-containing protein ACR4-like isoform X1 [Cucurbita maxima] >XP_023002813.1 ACT domain-containing protein ACR4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 875.2 bits (2260), Expect = 2.5e-250
Identity = 442/447 (98.88%), Postives = 445/447 (99.55%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD
Sbjct: 52  MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 111

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 112 LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 171

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 172 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 231

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQG+THIDRRLHQMMFADRDYEL+GEDLM
Sbjct: 232 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGVTHIDRRLHQMMFADRDYELLGEDLM 291

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 292 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGTEAYQ 351

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAEKQRVI+CLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 352 EYYIRHIDGSPVKSDAEKQRVIKCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 411

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420
           SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQG 
Sbjct: 412 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQG- 471

Query: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 472 SQDSPTRFLFGGLFRSRSFVNFGLIRS 497

BLAST of Carg20576 vs. ExPASy Swiss-Prot
Match: Q8LJW3 (ACT domain-containing protein ACR4 OS=Arabidopsis thaliana OX=3702 GN=ACR4 PE=2 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 9.3e-192
Identity = 345/450 (76.67%), Postives = 389/450 (86.44%), Query Frame = 0

Query: 5   MNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTDLNLI 64
           M+FS D+D+EY KLIRRMNP RVVIDN++CK ATVIRVDSAN++GILLEVVQ+LTDLNL 
Sbjct: 1   MSFSQDMDNEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLT 60

Query: 65  VTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVKPSVD 124
           +TKAYIS DG WFMDVFNVTDQDGNKVTDE VLDYI++SL  ++CF++SMRSVGV PS D
Sbjct: 61  ITKAYISSDGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTD 120

Query: 125 YTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAITDLE 184
            T IEL G DR GLLSELSAVLTHLKC+V++AE+WTHNTRAAAVM VTDD TG  I+D E
Sbjct: 121 STVIELTGCDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPE 180

Query: 185 RLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYE--LVGED--LM 244
           RLSRIK LL NVL+GSN     AKTVVS G  H DRRLHQMMF DRDYE  LV +D  + 
Sbjct: 181 RLSRIKNLLRNVLKGSNT-PREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDDSSIQ 240

Query: 245 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 304
           DE+QRP+V V NW D DYSV+T+R KDRPKLLFDT+CTLTDMQYVVFH +V  EG EA+Q
Sbjct: 241 DERQRPDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQ 300

Query: 305 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 364
           EYY+RHIDGSPVKS+AEKQRVIQCLEAAI+RRVSEGLKLELCTTDRVGLLS+VTRIFREN
Sbjct: 301 EYYVRHIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFREN 360

Query: 365 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQ-- 424
           SLTVTRAEV TKGGKA+NTFYV DASGY +D+KTIDSIR  IGQTILKVK +P+EQ+Q  
Sbjct: 361 SLTVTRAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQ 420

Query: 425 -GSSQDSPTRFLFGGLFRSRSFVNFGLIRS 448
              S +SPTRFLFGGLF+S+SFVNFGL+RS
Sbjct: 421 KSPSHESPTRFLFGGLFKSKSFVNFGLVRS 449

BLAST of Carg20576 vs. ExPASy Swiss-Prot
Match: Q9ZPQ8 (ACT domain-containing protein ACR5 OS=Arabidopsis thaliana OX=3702 GN=ACR5 PE=2 SV=2)

HSP 1 Score: 592.0 bits (1525), Expect = 5.5e-168
Identity = 317/458 (69.21%), Postives = 372/458 (81.22%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           M + +++S+++DDE  K IRR+NP RVVIDNE CKD TVI+VDSANKHGILLEVVQVLT+
Sbjct: 1   MDVCLSYSYNMDDEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTE 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESD--SCFASSMRS-V 120
           LNL + KAYIS DG WFMDVFNVTDQDGNKVTDE VL+YI++SL  D  SCF+ SMRS +
Sbjct: 61  LNLTIKKAYISSDGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTI 120

Query: 121 GVKPSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETG 180
           GVK SVDYT +EL G DR GLLSEL AVL  L+CNVV+AE+WTH  +AAAV+ VTD+ET 
Sbjct: 121 GVKQSVDYTVVELTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETC 180

Query: 181 SAITDLERLSRIKGLLCNVLRG--SNNRTNGAKTVVSQGI--THIDRRLHQMMFADRDYE 240
           SAITD ERLS+I+ LL  VL G  S  R    KT VS  +  TH DR+LHQ+MFADRDY+
Sbjct: 181 SAITDPERLSKIRKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYD 240

Query: 241 LVGEDLMDE----KQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHA 300
               ++ DE    +  P+V V N  D+DYS++ I+ KDRPKLLFDT+ TLTDM YVV HA
Sbjct: 241 EWENNVDDEDKCGRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHA 300

Query: 301 NVVAEGAEAYQEYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGL 360
           ++ AEG +AYQEYYIRH DGSPVKS+AE+QRVI+CL+AAI+RRVSEGLKLELCT+DRVGL
Sbjct: 301 SIDAEGPQAYQEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGL 360

Query: 361 LSDVTRIFRENSLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKV 420
           LSDVTRIFRENSLTVTRAEV TKG KA+NTFYVRDASGY VD+KTI+SIR +IGQTIL+V
Sbjct: 361 LSDVTRIFRENSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQV 420

Query: 421 KGSPKEQKQGSSQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           KG   + K  S QDSPT FLF G+F+SRSFVNFGLIRS
Sbjct: 421 KGGNTDAKP-SPQDSPTGFLF-GVFKSRSFVNFGLIRS 456

BLAST of Carg20576 vs. ExPASy Swiss-Prot
Match: Q9SGA0 (ACT domain-containing protein ACR6 OS=Arabidopsis thaliana OX=3702 GN=ACR6 PE=1 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 8.8e-142
Identity = 270/434 (62.21%), Postives = 329/434 (75.81%), Query Frame = 0

Query: 12  DDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTDLNLIVTKAYIS 71
           DDEY KLIRRMNP RVVIDN A  DATVI+VDS NKHG LLEVVQVLTD+NL++ KAYIS
Sbjct: 3   DDEYAKLIRRMNPPRVVIDNNASDDATVIQVDSVNKHGTLLEVVQVLTDMNLVIKKAYIS 62

Query: 72  CDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDS-CFASSMR-SVGVKPSVDYTAIE 131
            DG WFMDVF V DQDGNK+ D  VLDYI++ +ES++  F   +R SVGV P+ +YT+IE
Sbjct: 63  SDGGWFMDVFKVIDQDGNKIRDTQVLDYIQKRIESNAGWFIPPLRSSVGVMPTDEYTSIE 122

Query: 132 LIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAITDLERLSRI 191
           L G DR GLLSE+SAVLT L CNVV+AE+WTHNTRAAAV+HVTD+ T SAITD  RLS I
Sbjct: 123 LAGTDRPGLLSEVSAVLTDLHCNVVNAEIWTHNTRAAAVIHVTDNSTHSAITDPIRLSTI 182

Query: 192 KGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLMDEKQRPNVK 251
           K LLCNV+R +N+ +  AKTV S   TH +RRLHQ+MF DRDYE V +       RP+V 
Sbjct: 183 KELLCNVVR-TNSGSRAAKTVFSCSDTHRERRLHQIMFDDRDYEGV-KRARTSASRPSVT 242

Query: 252 VVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQEYYIRHIDG 311
           ++N  + DY+V+T+RSKDRPKL+FD +CTLTDMQYVVFH  V  E  EAYQE+YIRH+DG
Sbjct: 243 LMN-IEKDYTVVTMRSKDRPKLVFDVVCTLTDMQYVVFHGMVSTEPVEAYQEFYIRHVDG 302

Query: 312 SPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFRENSLTVTRAEV 371
            P+ S+AE++RVIQCLEAAI RR SEGL+LEL   DRVGLLSD+TR FRENSLT+ RAE+
Sbjct: 303 LPINSEAEQERVIQCLEAAIERRASEGLELELSAEDRVGLLSDITRTFRENSLTIVRAEI 362

Query: 372 STKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQ------KQGSSQD 431
           ST+ GKA +TFYV D +G PV+SK ++SIR  IG + LKVK   KE        + S + 
Sbjct: 363 STREGKAKDTFYVTDVTGNPVESKIVESIRQQIGVSKLKVKKKEKEHCSVLGTSRPSHET 422

Query: 432 SPTRFLFGGLFRSR 438
           +   +L   +F+ +
Sbjct: 423 TTMGYLLSNIFKPK 433

BLAST of Carg20576 vs. ExPASy Swiss-Prot
Match: O49285 (ACT domain-containing protein ACR3 OS=Arabidopsis thaliana OX=3702 GN=ACR3 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 3.4e-117
Identity = 234/438 (53.42%), Postives = 299/438 (68.26%), Query Frame = 0

Query: 12  DDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTDLNLIVTKAYIS 71
           D EY  L  R+NP  V IDN +CK+ T+++VDS NK GILLEVVQVLTDL+L +TKAYIS
Sbjct: 10  DPEYENLSSRINPPSVSIDNTSCKECTLVKVDSMNKPGILLEVVQVLTDLDLTITKAYIS 69

Query: 72  CDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSM-----RSVGVKPSVDYT 131
            DG WFMDVF+VTDQ GNKVTD   +DYI++ L      ++S      + VGV    D+T
Sbjct: 70  SDGGWFMDVFHVTDQQGNKVTDSKTIDYIEKVLGPKGHASASQNTWPGKRVGVHSLGDHT 129

Query: 132 AIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAITDLERL 191
           +IE+I  DR GLLSE+SAVL  L  NVV AE WTHN R A V++V D+ T  A+ D ERL
Sbjct: 130 SIEIIARDRPGLLSEVSAVLADLNINVVAAEAWTHNRRIACVLYVNDNATSRAVDDPERL 189

Query: 192 SRIKGLLCNVLRGSNNRTNG-AKTVVSQGITHIDRRLHQMMFADRDYELVG--EDLMDEK 251
           S ++  L NVLRG   +    A+T +S G TH+DRRLHQM FADRDYE V   +D     
Sbjct: 190 SSMEEQLNNVLRGCEEQDEKFARTSLSIGSTHVDRRLHQMFFADRDYEAVTKLDDSASCG 249

Query: 252 QRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQEYY 311
             P + V +  +  YSVI +  +DRPKL+FD +CTLTDMQY+VFHA + + G+ A QEY+
Sbjct: 250 FEPKITVEHCEEKGYSVINVSCEDRPKLMFDIVCTLTDMQYIVFHATISSSGSHASQEYF 309

Query: 312 IRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFRENSLT 371
           IRH DG  + ++ EK+RV++CLEAAI RRVSEG  LELC  DRVGLLS+VTRI RE+ L+
Sbjct: 310 IRHKDGCTLDTEGEKERVVKCLEAAIHRRVSEGWSLELCAKDRVGLLSEVTRILREHGLS 369

Query: 372 VTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTIL-----KVKGSP-KEQK 431
           V+RA V+T G +A+N FYV+DASG PVD KTI+++R  IG +++     KV     KE+ 
Sbjct: 370 VSRAGVTTVGEQAVNVFYVKDASGNPVDVKTIEALRGEIGHSMMIDFKNKVPSRKWKEEG 429

Query: 432 QGSSQD--SPTRFLFGGL 434
           Q  +    + T F FG L
Sbjct: 430 QAGTGGGWAKTSFFFGNL 447

BLAST of Carg20576 vs. ExPASy Swiss-Prot
Match: Q9LNA5 (ACT domain-containing protein ACR8 OS=Arabidopsis thaliana OX=3702 GN=ACR8 PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.2e-111
Identity = 221/440 (50.23%), Postives = 294/440 (66.82%), Query Frame = 0

Query: 13  DEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTDLNLIVTKAYISC 72
           DEY KL+ RMN  RVVIDN  C  AT+++VDS+ ++GILLE VQ+LTDLNL + KAYIS 
Sbjct: 8   DEYEKLVIRMNTPRVVIDNGVCSSATIVKVDSSRRNGILLEAVQILTDLNLSIKKAYISS 67

Query: 73  DGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVKPSVDYTAIELIG 132
           DG W MDVF+VTD +GNK+ D+ VL YI++S+E+   +  ++   G+      TA+EL G
Sbjct: 68  DGTWNMDVFHVTDLNGNKLNDQSVLRYIEQSIET-VYYGENIEVNGL------TALELTG 127

Query: 133 NDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAITDLERLSRIKGL 192
            DR GLLSE+ AVL+ L C+VV A++WTHN R A+V+++ D  +G+ I D  R+S+I+G 
Sbjct: 128 TDRIGLLSEMFAVLSDLNCDVVDAKLWTHNGRVASVIYLKDCISGAPILDSHRISKIEGR 187

Query: 193 LCNVLRGSNNRTNGAKTVVS-QGITHIDRRLHQMMFADRDYELVGEDLMDEKQRPNVKVV 252
           L NVL G N+  + AKT V+   + HI+RRLHQ+MF DRDYE   +   +      V V 
Sbjct: 188 LKNVLNGDNDVNSAAKTCVTVDSMMHIERRLHQLMFEDRDYERRSKK-HERSPMVVVTVQ 247

Query: 253 NWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQEYYIRHIDGSP 312
           NW +  YSV+ +  +DR KLLFD +CTLTDM+Y VFHA +     +A+ E+YIRH DGSP
Sbjct: 248 NWAERGYSVVNVHCRDRTKLLFDVVCTLTDMEYAVFHATINTAEDQAHLEFYIRHKDGSP 307

Query: 313 VKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFRENSLTVTRAEVST 372
           + S+AE+QRVIQCLEAA+ RR  EG++LEL   D+ GLL++VTR FREN L VTR E+ST
Sbjct: 308 ISSEAERQRVIQCLEAAVERRALEGVRLELRHPDKQGLLAEVTRTFRENGLNVTRTEIST 367

Query: 373 KGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSP---KEQKQGSSQDSPTRF 432
               A N FYV DA+G   D K I+S+R+ IG   L+VK  P    ++  G  Q      
Sbjct: 368 SSDMATNIFYVTDANGDEPDFKLIESVREKIGLECLRVKEMPTMYHKKGDGEEQQQTKAV 427

Query: 433 LFG-GLFRSRSFVNFGLIRS 448
           L   G    R+  NFGLI+S
Sbjct: 428 LVSLGSLVWRNLFNFGLIKS 439

BLAST of Carg20576 vs. ExPASy TrEMBL
Match: A0A6J1GHG6 (ACT domain-containing protein ACR4-like OS=Cucurbita moschata OX=3662 GN=LOC111453982 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 5.7e-253
Identity = 445/447 (99.55%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD
Sbjct: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYEL+GEDLM
Sbjct: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELLGEDLM 240

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGTEAYQ 300

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420
           SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS
Sbjct: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420

Query: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 447

BLAST of Carg20576 vs. ExPASy TrEMBL
Match: A0A6J1KUN7 (ACT domain-containing protein ACR4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496566 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.2e-250
Identity = 442/447 (98.88%), Postives = 445/447 (99.55%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD
Sbjct: 52  MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 111

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 112 LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 171

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 172 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 231

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQG+THIDRRLHQMMFADRDYEL+GEDLM
Sbjct: 232 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGVTHIDRRLHQMMFADRDYELLGEDLM 291

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 292 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGTEAYQ 351

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAEKQRVI+CLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 352 EYYIRHIDGSPVKSDAEKQRVIKCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 411

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420
           SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQG 
Sbjct: 412 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQG- 471

Query: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 472 SQDSPTRFLFGGLFRSRSFVNFGLIRS 497

BLAST of Carg20576 vs. ExPASy TrEMBL
Match: A0A6J1KRI7 (ACT domain-containing protein ACR4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496566 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.2e-250
Identity = 442/447 (98.88%), Postives = 445/447 (99.55%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD
Sbjct: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQG+THIDRRLHQMMFADRDYEL+GEDLM
Sbjct: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGVTHIDRRLHQMMFADRDYELLGEDLM 240

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGTEAYQ 300

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAEKQRVI+CLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 301 EYYIRHIDGSPVKSDAEKQRVIKCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQGS 420
           SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQG 
Sbjct: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQG- 420

Query: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 421 SQDSPTRFLFGGLFRSRSFVNFGLIRS 446

BLAST of Carg20576 vs. ExPASy TrEMBL
Match: A0A5A7VC80 (ACT domain-containing protein ACR4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00870 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.4e-238
Identity = 421/449 (93.76%), Postives = 437/449 (97.33%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNP RVVIDN+ACKDATVIRVDSANKHGILLE VQVLTD
Sbjct: 1   MAIDMNFSHDIDDEYVKLIRRMNPPRVVIDNDACKDATVIRVDSANKHGILLEAVQVLTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSE+SAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 121 PSVDYTAIELIGNDRQGLLSEVSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLL NVLRGSN+R+NGAKTVVS G+THI+RRLHQMMFADRDYEL+ ED M
Sbjct: 181 TDLERLSRIKGLLSNVLRGSNSRSNGAKTVVSHGVTHIERRLHQMMFADRDYELLDEDAM 240

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           +++QRPNVKVVNWCDIDYSV+TIRSKDRPKLLFDT+CTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 241 EDQQRPNVKVVNWCDIDYSVVTIRSKDRPKLLFDTVCTLTDMQYVVFHANVVAEGTEAYQ 300

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAE+QRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 301 EYYIRHIDGSPVKSDAERQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKG--SPKEQKQ 420
           SLTVTRAEV+TK GKAINTFYVRD SGYPVDSKTIDSIR+LIGQTILKVKG  SPKEQKQ
Sbjct: 361 SLTVTRAEVTTKAGKAINTFYVRDPSGYPVDSKTIDSIRELIGQTILKVKGSSSPKEQKQ 420

Query: 421 GSSQDSPTRFLFGGLFRSRSFVNFGLIRS 448
            +SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 421 -ASQDSPTRFLFGGLFRSRSFVNFGLIRS 448

BLAST of Carg20576 vs. ExPASy TrEMBL
Match: A0A1S3BCP8 (ACT domain-containing protein ACR4-like OS=Cucumis melo OX=3656 GN=LOC103488452 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.4e-238
Identity = 421/449 (93.76%), Postives = 437/449 (97.33%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           MAIDMNFSHDIDDEYVKLIRRMNP RVVIDN+ACKDATVIRVDSANKHGILLE VQVLTD
Sbjct: 1   MAIDMNFSHDIDDEYVKLIRRMNPPRVVIDNDACKDATVIRVDSANKHGILLEAVQVLTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK
Sbjct: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PSVDYTAIELIGNDRQGLLSE+SAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI
Sbjct: 121 PSVDYTAIELIGNDRQGLLSEVSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLM 240
           TDLERLSRIKGLL NVLRGSN+R+NGAKTVVS G+THI+RRLHQMMFADRDYEL+ ED M
Sbjct: 181 TDLERLSRIKGLLSNVLRGSNSRSNGAKTVVSHGVTHIERRLHQMMFADRDYELLDEDAM 240

Query: 241 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 300
           +++QRPNVKVVNWCDIDYSV+TIRSKDRPKLLFDT+CTLTDMQYVVFHANVVAEG EAYQ
Sbjct: 241 EDQQRPNVKVVNWCDIDYSVVTIRSKDRPKLLFDTVCTLTDMQYVVFHANVVAEGTEAYQ 300

Query: 301 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360
           EYYIRHIDGSPVKSDAE+QRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN
Sbjct: 301 EYYIRHIDGSPVKSDAERQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 360

Query: 361 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKG--SPKEQKQ 420
           SLTVTRAEV+TK GKAINTFYVRD SGYPVDSKTIDSIR+LIGQTILKVKG  SPKEQKQ
Sbjct: 361 SLTVTRAEVTTKAGKAINTFYVRDPSGYPVDSKTIDSIRELIGQTILKVKGSSSPKEQKQ 420

Query: 421 GSSQDSPTRFLFGGLFRSRSFVNFGLIRS 448
            +SQDSPTRFLFGGLFRSRSFVNFGLIRS
Sbjct: 421 -ASQDSPTRFLFGGLFRSRSFVNFGLIRS 448

BLAST of Carg20576 vs. TAIR 10
Match: AT1G69040.2 (ACT domain repeat 4 )

HSP 1 Score: 673.3 bits (1736), Expect = 1.3e-193
Identity = 346/454 (76.21%), Postives = 391/454 (86.12%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           M + M+FS D+D+EY KLIRRMNP RVVIDN++CK ATVIRVDSAN++GILLEVVQ+LTD
Sbjct: 1   MDVSMSFSQDMDNEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTD 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVK 120
           LNL +TKAYIS DG WFMDVFNVTDQDGNKVTDE VLDYI++SL  ++CF++SMRSVGV 
Sbjct: 61  LNLTITKAYISSDGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVI 120

Query: 121 PSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAI 180
           PS D T IEL G DR GLLSELSAVLTHLKC+V++AE+WTHNTRAAAVM VTDD TG  I
Sbjct: 121 PSTDSTVIELTGCDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGI 180

Query: 181 TDLERLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYE--LVGED 240
           +D ERLSRIK LL NVL+GSN     AKTVVS G  H DRRLHQMMF DRDYE  LV +D
Sbjct: 181 SDPERLSRIKNLLRNVLKGSNT-PREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDD 240

Query: 241 --LMDEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGA 300
             + DE+QRP+V V NW D DYSV+T+R KDRPKLLFDT+CTLTDMQYVVFH +V  EG 
Sbjct: 241 SSIQDERQRPDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGT 300

Query: 301 EAYQEYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRI 360
           EA+QEYY+RHIDGSPVKS+AEKQRVIQCLEAAI+RRVSEGLKLELCTTDRVGLLS+VTRI
Sbjct: 301 EAFQEYYVRHIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRI 360

Query: 361 FRENSLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQ 420
           FRENSLTVTRAEV TKGGKA+NTFYV DASGY +D+KTIDSIR  IGQTILKVK +P+EQ
Sbjct: 361 FRENSLTVTRAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQ 420

Query: 421 KQ---GSSQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           +Q     S +SPTRFLFGGLF+S+SFVNFGL+RS
Sbjct: 421 QQRQKSPSHESPTRFLFGGLFKSKSFVNFGLVRS 453

BLAST of Carg20576 vs. TAIR 10
Match: AT1G69040.1 (ACT domain repeat 4 )

HSP 1 Score: 671.0 bits (1730), Expect = 6.6e-193
Identity = 345/450 (76.67%), Postives = 389/450 (86.44%), Query Frame = 0

Query: 5   MNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTDLNLI 64
           M+FS D+D+EY KLIRRMNP RVVIDN++CK ATVIRVDSAN++GILLEVVQ+LTDLNL 
Sbjct: 1   MSFSQDMDNEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLT 60

Query: 65  VTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDSCFASSMRSVGVKPSVD 124
           +TKAYIS DG WFMDVFNVTDQDGNKVTDE VLDYI++SL  ++CF++SMRSVGV PS D
Sbjct: 61  ITKAYISSDGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTD 120

Query: 125 YTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAITDLE 184
            T IEL G DR GLLSELSAVLTHLKC+V++AE+WTHNTRAAAVM VTDD TG  I+D E
Sbjct: 121 STVIELTGCDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPE 180

Query: 185 RLSRIKGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYE--LVGED--LM 244
           RLSRIK LL NVL+GSN     AKTVVS G  H DRRLHQMMF DRDYE  LV +D  + 
Sbjct: 181 RLSRIKNLLRNVLKGSNT-PREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDDSSIQ 240

Query: 245 DEKQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQ 304
           DE+QRP+V V NW D DYSV+T+R KDRPKLLFDT+CTLTDMQYVVFH +V  EG EA+Q
Sbjct: 241 DERQRPDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQ 300

Query: 305 EYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFREN 364
           EYY+RHIDGSPVKS+AEKQRVIQCLEAAI+RRVSEGLKLELCTTDRVGLLS+VTRIFREN
Sbjct: 301 EYYVRHIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFREN 360

Query: 365 SLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQKQ-- 424
           SLTVTRAEV TKGGKA+NTFYV DASGY +D+KTIDSIR  IGQTILKVK +P+EQ+Q  
Sbjct: 361 SLTVTRAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQ 420

Query: 425 -GSSQDSPTRFLFGGLFRSRSFVNFGLIRS 448
              S +SPTRFLFGGLF+S+SFVNFGL+RS
Sbjct: 421 KSPSHESPTRFLFGGLFKSKSFVNFGLVRS 449

BLAST of Carg20576 vs. TAIR 10
Match: AT2G03730.1 (ACT domain repeat 5 )

HSP 1 Score: 592.0 bits (1525), Expect = 3.9e-169
Identity = 317/458 (69.21%), Postives = 372/458 (81.22%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           M + +++S+++DDE  K IRR+NP RVVIDNE CKD TVI+VDSANKHGILLEVVQVLT+
Sbjct: 1   MDVCLSYSYNMDDEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTE 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESD--SCFASSMRS-V 120
           LNL + KAYIS DG WFMDVFNVTDQDGNKVTDE VL+YI++SL  D  SCF+ SMRS +
Sbjct: 61  LNLTIKKAYISSDGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTI 120

Query: 121 GVKPSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETG 180
           GVK SVDYT +EL G DR GLLSEL AVL  L+CNVV+AE+WTH  +AAAV+ VTD+ET 
Sbjct: 121 GVKQSVDYTVVELTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETC 180

Query: 181 SAITDLERLSRIKGLLCNVLRG--SNNRTNGAKTVVSQGI--THIDRRLHQMMFADRDYE 240
           SAITD ERLS+I+ LL  VL G  S  R    KT VS  +  TH DR+LHQ+MFADRDY+
Sbjct: 181 SAITDPERLSKIRKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYD 240

Query: 241 LVGEDLMDE----KQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHA 300
               ++ DE    +  P+V V N  D+DYS++ I+ KDRPKLLFDT+ TLTDM YVV HA
Sbjct: 241 EWENNVDDEDKCGRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHA 300

Query: 301 NVVAEGAEAYQEYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGL 360
           ++ AEG +AYQEYYIRH DGSPVKS+AE+QRVI+CL+AAI+RRVSEGLKLELCT+DRVGL
Sbjct: 301 SIDAEGPQAYQEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGL 360

Query: 361 LSDVTRIFRENSLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKV 420
           LSDVTRIFRENSLTVTRAEV TKG KA+NTFYVRDASGY VD+KTI+SIR +IGQTIL+V
Sbjct: 361 LSDVTRIFRENSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQV 420

Query: 421 KGSPKEQKQGSSQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           KG   + K  S QDSPT FLF G+F+SRSFVNFGLIRS
Sbjct: 421 KGGNTDAKP-SPQDSPTGFLF-GVFKSRSFVNFGLIRS 456

BLAST of Carg20576 vs. TAIR 10
Match: AT2G03730.2 (ACT domain repeat 5 )

HSP 1 Score: 592.0 bits (1525), Expect = 3.9e-169
Identity = 317/458 (69.21%), Postives = 372/458 (81.22%), Query Frame = 0

Query: 1   MAIDMNFSHDIDDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTD 60
           M + +++S+++DDE  K IRR+NP RVVIDNE CKD TVI+VDSANKHGILLEVVQVLT+
Sbjct: 1   MDVCLSYSYNMDDEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTE 60

Query: 61  LNLIVTKAYISCDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESD--SCFASSMRS-V 120
           LNL + KAYIS DG WFMDVFNVTDQDGNKVTDE VL+YI++SL  D  SCF+ SMRS +
Sbjct: 61  LNLTIKKAYISSDGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTI 120

Query: 121 GVKPSVDYTAIELIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETG 180
           GVK SVDYT +EL G DR GLLSEL AVL  L+CNVV+AE+WTH  +AAAV+ VTD+ET 
Sbjct: 121 GVKQSVDYTVVELTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETC 180

Query: 181 SAITDLERLSRIKGLLCNVLRG--SNNRTNGAKTVVSQGI--THIDRRLHQMMFADRDYE 240
           SAITD ERLS+I+ LL  VL G  S  R    KT VS  +  TH DR+LHQ+MFADRDY+
Sbjct: 181 SAITDPERLSKIRKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYD 240

Query: 241 LVGEDLMDE----KQRPNVKVVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHA 300
               ++ DE    +  P+V V N  D+DYS++ I+ KDRPKLLFDT+ TLTDM YVV HA
Sbjct: 241 EWENNVDDEDKCGRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHA 300

Query: 301 NVVAEGAEAYQEYYIRHIDGSPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGL 360
           ++ AEG +AYQEYYIRH DGSPVKS+AE+QRVI+CL+AAI+RRVSEGLKLELCT+DRVGL
Sbjct: 301 SIDAEGPQAYQEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGL 360

Query: 361 LSDVTRIFRENSLTVTRAEVSTKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKV 420
           LSDVTRIFRENSLTVTRAEV TKG KA+NTFYVRDASGY VD+KTI+SIR +IGQTIL+V
Sbjct: 361 LSDVTRIFRENSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQV 420

Query: 421 KGSPKEQKQGSSQDSPTRFLFGGLFRSRSFVNFGLIRS 448
           KG   + K  S QDSPT FLF G+F+SRSFVNFGLIRS
Sbjct: 421 KGGNTDAKP-SPQDSPTGFLF-GVFKSRSFVNFGLIRS 456

BLAST of Carg20576 vs. TAIR 10
Match: AT3G01990.1 (ACT domain repeat 6 )

HSP 1 Score: 505.0 bits (1299), Expect = 6.3e-143
Identity = 270/434 (62.21%), Postives = 329/434 (75.81%), Query Frame = 0

Query: 12  DDEYVKLIRRMNPTRVVIDNEACKDATVIRVDSANKHGILLEVVQVLTDLNLIVTKAYIS 71
           DDEY KLIRRMNP RVVIDN A  DATVI+VDS NKHG LLEVVQVLTD+NL++ KAYIS
Sbjct: 3   DDEYAKLIRRMNPPRVVIDNNASDDATVIQVDSVNKHGTLLEVVQVLTDMNLVIKKAYIS 62

Query: 72  CDGCWFMDVFNVTDQDGNKVTDEGVLDYIKRSLESDS-CFASSMR-SVGVKPSVDYTAIE 131
            DG WFMDVF V DQDGNK+ D  VLDYI++ +ES++  F   +R SVGV P+ +YT+IE
Sbjct: 63  SDGGWFMDVFKVIDQDGNKIRDTQVLDYIQKRIESNAGWFIPPLRSSVGVMPTDEYTSIE 122

Query: 132 LIGNDRQGLLSELSAVLTHLKCNVVHAEVWTHNTRAAAVMHVTDDETGSAITDLERLSRI 191
           L G DR GLLSE+SAVLT L CNVV+AE+WTHNTRAAAV+HVTD+ T SAITD  RLS I
Sbjct: 123 LAGTDRPGLLSEVSAVLTDLHCNVVNAEIWTHNTRAAAVIHVTDNSTHSAITDPIRLSTI 182

Query: 192 KGLLCNVLRGSNNRTNGAKTVVSQGITHIDRRLHQMMFADRDYELVGEDLMDEKQRPNVK 251
           K LLCNV+R +N+ +  AKTV S   TH +RRLHQ+MF DRDYE V +       RP+V 
Sbjct: 183 KELLCNVVR-TNSGSRAAKTVFSCSDTHRERRLHQIMFDDRDYEGV-KRARTSASRPSVT 242

Query: 252 VVNWCDIDYSVITIRSKDRPKLLFDTICTLTDMQYVVFHANVVAEGAEAYQEYYIRHIDG 311
           ++N  + DY+V+T+RSKDRPKL+FD +CTLTDMQYVVFH  V  E  EAYQE+YIRH+DG
Sbjct: 243 LMN-IEKDYTVVTMRSKDRPKLVFDVVCTLTDMQYVVFHGMVSTEPVEAYQEFYIRHVDG 302

Query: 312 SPVKSDAEKQRVIQCLEAAIRRRVSEGLKLELCTTDRVGLLSDVTRIFRENSLTVTRAEV 371
            P+ S+AE++RVIQCLEAAI RR SEGL+LEL   DRVGLLSD+TR FRENSLT+ RAE+
Sbjct: 303 LPINSEAEQERVIQCLEAAIERRASEGLELELSAEDRVGLLSDITRTFRENSLTIVRAEI 362

Query: 372 STKGGKAINTFYVRDASGYPVDSKTIDSIRDLIGQTILKVKGSPKEQ------KQGSSQD 431
           ST+ GKA +TFYV D +G PV+SK ++SIR  IG + LKVK   KE        + S + 
Sbjct: 363 STREGKAKDTFYVTDVTGNPVESKIVESIRQQIGVSKLKVKKKEKEHCSVLGTSRPSHET 422

Query: 432 SPTRFLFGGLFRSR 438
           +   +L   +F+ +
Sbjct: 423 TTMGYLLSNIFKPK 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7020178.11.8e-253100.00ACT domain-containing protein ACR4 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6585256.15.3e-25399.78ACT domain-containing protein ACR4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022951004.11.2e-25299.55ACT domain-containing protein ACR4-like [Cucurbita moschata] >XP_022951006.1 ACT... [more]
XP_023002814.12.5e-25098.88ACT domain-containing protein ACR4-like isoform X2 [Cucurbita maxima][more]
XP_023002812.12.5e-25098.88ACT domain-containing protein ACR4-like isoform X1 [Cucurbita maxima] >XP_023002... [more]
Match NameE-valueIdentityDescription
Q8LJW39.3e-19276.67ACT domain-containing protein ACR4 OS=Arabidopsis thaliana OX=3702 GN=ACR4 PE=2 ... [more]
Q9ZPQ85.5e-16869.21ACT domain-containing protein ACR5 OS=Arabidopsis thaliana OX=3702 GN=ACR5 PE=2 ... [more]
Q9SGA08.8e-14262.21ACT domain-containing protein ACR6 OS=Arabidopsis thaliana OX=3702 GN=ACR6 PE=1 ... [more]
O492853.4e-11753.42ACT domain-containing protein ACR3 OS=Arabidopsis thaliana OX=3702 GN=ACR3 PE=2 ... [more]
Q9LNA51.2e-11150.23ACT domain-containing protein ACR8 OS=Arabidopsis thaliana OX=3702 GN=ACR8 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GHG65.7e-25399.55ACT domain-containing protein ACR4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KUN71.2e-25098.88ACT domain-containing protein ACR4-like isoform X1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1KRI71.2e-25098.88ACT domain-containing protein ACR4-like isoform X2 OS=Cucurbita maxima OX=3661 G... [more]
A0A5A7VC801.4e-23893.76ACT domain-containing protein ACR4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BCP81.4e-23893.76ACT domain-containing protein ACR4-like OS=Cucumis melo OX=3656 GN=LOC103488452 ... [more]
Match NameE-valueIdentityDescription
AT1G69040.21.3e-19376.21ACT domain repeat 4 [more]
AT1G69040.16.6e-19376.67ACT domain repeat 4 [more]
AT2G03730.13.9e-16969.21ACT domain repeat 5 [more]
AT2G03730.23.9e-16969.21ACT domain repeat 5 [more]
AT3G01990.16.3e-14362.21ACT domain repeat 6 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.260coord: 125..198
e-value: 1.3E-9
score: 40.1
NoneNo IPR availablePFAMPF13740ACT_6coord: 128..191
e-value: 2.2E-5
score: 24.4
NoneNo IPR availablePANTHERPTHR31096:SF69BNAC06G29950D PROTEINcoord: 6..446
NoneNo IPR availableCDDcd04897ACT_ACR_3coord: 258..332
e-value: 6.91741E-38
score: 130.619
NoneNo IPR availableCDDcd04926ACT_ACR_4coord: 336..407
e-value: 2.10847E-35
score: 123.999
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 334..403
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 124..184
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 34..103
IPR002912ACT domainPFAMPF01842ACTcoord: 344..398
e-value: 2.1E-6
score: 27.4
coord: 38..89
e-value: 7.0E-7
score: 28.9
IPR002912ACT domainPROSITEPS51671ACTcoord: 127..207
score: 9.91972
IPR002912ACT domainPROSITEPS51671ACTcoord: 39..119
score: 9.521967
IPR002912ACT domainPROSITEPS51671ACTcoord: 338..414
score: 12.719292
IPR040217ACT domain-containing protein ACR1-12PANTHERPTHR31096ACT DOMAIN-CONTAINING PROTEIN ACR4-RELATEDcoord: 6..446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20576-RACarg20576-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016779 nucleotidyltransferase activity