Carg20540 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg20540 vs. NCBI nr
Match: KAG7020214.1 (putative mitochondrial adenine nucleotide transporter BTL3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1909.8 bits (4946), Expect = 0.0e+00
Identity = 964/964 (100.00%), Postives = 964/964 (100.00%), Query Frame = 0

Query: 1   MPRPELGSCSSFFQGPSQSQSLSCCSTNSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEE 60
           MPRPELGSCSSFFQGPSQSQSLSCCSTNSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEE
Sbjct: 1   MPRPELGSCSSFFQGPSQSQSLSCCSTNSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEE 60

Query: 61  SGSVSVSASFCHQEQPVFFGVLRWRRKPRIADCGFLSLSVRGDRFVPEQKVRADGYENCE 120
           SGSVSVSASFCHQEQPVFFGVLRWRRKPRIADCGFLSLSVRGDRFVPEQKVRADGYENCE
Sbjct: 61  SGSVSVSASFCHQEQPVFFGVLRWRRKPRIADCGFLSLSVRGDRFVPEQKVRADGYENCE 120

Query: 121 KAGKCEGALATVENDKQEKKYTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYI 180
           KAGKCEGALATVENDKQEKKYTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYI
Sbjct: 121 KAGKCEGALATVENDKQEKKYTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYI 180

Query: 181 VRGEQGNLFYVIKKIAASQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRE 240
           VRGEQGNLFYVIKKIAASQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRE
Sbjct: 181 VRGEQGNLFYVIKKIAASQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRE 240

Query: 241 TTSNFERFVAGAAAGITATVLCLPLDTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLY 300
           TTSNFERFVAGAAAGITATVLCLPLDTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLY
Sbjct: 241 TTSNFERFVAGAAAGITATVLCLPLDTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLY 300

Query: 301 KGLLPSILSMAPSGAVFYTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIR 360
           KGLLPSILSMAPSGAVFYTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIR
Sbjct: 301 KGLLPSILSMAPSGAVFYTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIR 360

Query: 361 TLLYGAISGACAEAATYPFEVIRRQLQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPS 420
           TLLYGAISGACAEAATYPFEVIRRQLQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPS
Sbjct: 361 TLLYGAISGACAEAATYPFEVIRRQLQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPS 420

Query: 421 LLQACDSDLSFTFLSFILSRLFNRNSQFSAAVAVMSPGGCACIAIENGDAEASGNCKSGG 480
           LLQACDSDLSFTFLSFILSRLFNRNSQFSAAVAVMSPGGCACIAIENGDAEASGNCKSGG
Sbjct: 421 LLQACDSDLSFTFLSFILSRLFNRNSQFSAAVAVMSPGGCACIAIENGDAEASGNCKSGG 480

Query: 481 SYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDSARLRESGKFWRILVASAKGFTIGAGL 540
           SYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDSARLRESGKFWRILVASAKGFTIGAGL
Sbjct: 481 SYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDSARLRESGKFWRILVASAKGFTIGAGL 540

Query: 541 KGGLSLFSILAGLKRRKALASLGKKGVITNRDAISMALKETLRYGLFLGTFAGTFVSIDE 600
           KGGLSLFSILAGLKRRKALASLGKKGVITNRDAISMALKETLRYGLFLGTFAGTFVSIDE
Sbjct: 541 KGGLSLFSILAGLKRRKALASLGKKGVITNRDAISMALKETLRYGLFLGTFAGTFVSIDE 600

Query: 601 IIGNLAGHRRTARWRALSAGALAGPSMLLTGLNTQHKTLAIYIFMRAAVLAARCGIKSKR 660
           IIGNLAGHRRTARWRALSAGALAGPSMLLTGLNTQHKTLAIYIFMRAAVLAARCGIKSKR
Sbjct: 601 IIGNLAGHRRTARWRALSAGALAGPSMLLTGLNTQHKTLAIYIFMRAAVLAARCGIKSKR 660

Query: 661 FGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIFRSAYVLKQDSLPPSFSSFLNIHGGKD 720
           FGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIFRSAYVLKQDSLPPSFSSFLNIHGGKD
Sbjct: 661 FGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIFRSAYVLKQDSLPPSFSSFLNIHGGKD 720

Query: 721 TVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQMKTPCTIIHGNQSCGGHVLNF 780
           TVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQMKTPCTIIHGNQSCGGHVLNF
Sbjct: 721 TVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQMKTPCTIIHGNQSCGGHVLNF 780

Query: 781 LIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGLLGTARSSLFLSVYCSSAWMW 840
           LIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGLLGTARSSLFLSVYCSSAWMW
Sbjct: 781 LIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGLLGTARSSLFLSVYCSSAWMW 840

Query: 841 TCLTARTFKKINIPLVALATFLAGLALAIEKKSRRIEISLYCLARGIESFFSCMTDLGYL 900
           TCLTARTFKKINIPLVALATFLAGLALAIEKKSRRIEISLYCLARGIESFFSCMTDLGYL
Sbjct: 841 TCLTARTFKKINIPLVALATFLAGLALAIEKKSRRIEISLYCLARGIESFFSCMTDLGYL 900

Query: 901 PQSLNFKRADVIIFSISTAIIMHCYAQEREVFRSKYLNVLDWVFGVPPPPPCDETPSCKN 960
           PQSLNFKRADVIIFSISTAIIMHCYAQEREVFRSKYLNVLDWVFGVPPPPPCDETPSCKN
Sbjct: 901 PQSLNFKRADVIIFSISTAIIMHCYAQEREVFRSKYLNVLDWVFGVPPPPPCDETPSCKN 960

Query: 961 CKQH 965
           CKQH
Sbjct: 961 CKQH 964

BLAST of Carg20540 vs. NCBI nr
Match: KAB2628969.1 (hypothetical protein D8674_033764 [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 611/980 (62.35%), Postives = 712/980 (72.65%), Query Frame = 0

Query: 28  NSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEESGSVSVSASFCHQEQPVFFGVLRWRRK 87
           N   F+TGGLF++  VP+S  NS+ S   +   +  VS                      
Sbjct: 5   NPTLFITGGLFLEPTVPNSLLNSVVSFQKNSPSAAVVSGGGG------------------ 64

Query: 88  PRIADCGFLSLSVRGDRFVPEQK--VRADGYENCEKAGKCEGALATVENDKQEKKYTVHK 147
                 GF S+S+  D  V E K  +  +G E  E     E     V  +++ +K  V  
Sbjct: 65  -----GGFFSVSLSSDGVVRESKGCLVHNGDEGSE-----EAEAEAVVTERKREKVGVRS 124

Query: 148 RNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFYVIKKIAASQGLKGFW 207
             AMNTTKHLW+GAIAAMVSRTFVAPLERLKLEYIVRGEQ +LF +++ IAA+QGL+GFW
Sbjct: 125 GGAMNTTKHLWAGAIAAMVSRTFVAPLERLKLEYIVRGEQRHLFELVRTIAANQGLRGFW 184

Query: 208 KGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVAGAAAGITATVLCLPL 267
           KGN VNILRTAPFKA+NFYAYDTYRKQLL++SG + T+NFERFVAGAAAGITAT+LCLPL
Sbjct: 185 KGNLVNILRTAPFKAINFYAYDTYRKQLLRVSGNKETTNFERFVAGAAAGITATILCLPL 244

Query: 268 DTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSMAPSGAVFYTVYDILK 327
           DTIRT++VAPGGEALGGVIGAFR+M+QTEGFFSLYKGL+PSI SMAPSGAVFY VYDILK
Sbjct: 245 DTIRTQLVAPGGEALGGVIGAFRHMIQTEGFFSLYKGLVPSIASMAPSGAVFYGVYDILK 304

Query: 328 TAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGACAEAATYPFEVIRRQ 387
           +AYL SP+GRKRI  M+QQG+  +ALDQLELGPIRTLLYGAISGACAEAATYPFEV+RRQ
Sbjct: 305 SAYLHSPDGRKRIHKMSQQGQGRSALDQLELGPIRTLLYGAISGACAEAATYPFEVVRRQ 364

Query: 388 LQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPSLLQ---------------------- 447
           LQ+QVQ TRMSA AT  KIV+ GG+ ALYAGL+PSLLQ                      
Sbjct: 365 LQLQVQETRMSALATGLKIVEHGGVPALYAGLIPSLLQVLPSAAISYFVYEFMKIVLKFK 424

Query: 448 ------ACDS---------DLSFTFLSFILSRLFNRNS----QFSAAVAVMSPGGCACIA 507
                 AC S          L F+FLS+  S     +S     +S A  +     C C A
Sbjct: 425 PPKLSDACASLARPVDYRKALPFSFLSYEPSSASQPHSCMQGPYSFAAMLPDRDLCTCFA 484

Query: 508 IENGDAEASGNCKSGGSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDSARLRESGKFW 567
            ENG  +   +  S    C HC     S S+ Y         S SL   SA L +S K  
Sbjct: 485 GENGGPDGDRH-GSADPNCTHCRKSDPSPSSLY--------YSSSLGYVSAPLSDSEKLR 544

Query: 568 RILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAISMALKETLRY 627
           R++ AS KGFTIGAGLKGGL++FSILA L+RRK LASL  +GVITNR+AI  ALKETLRY
Sbjct: 545 RVVTASVKGFTIGAGLKGGLAIFSILARLRRRKLLASLRTEGVITNREAIVTALKETLRY 604

Query: 628 GLFLGTFAGTFVSIDEIIGNLAGHR---RTARWRALSAGALAGPSMLLTGLNTQHKTLAI 687
           GLFLGTFAGTFVS+DEIIG+LAGHR   RTA+WRAL AGA+AGPSMLLTGLNTQH +LAI
Sbjct: 605 GLFLGTFAGTFVSVDEIIGSLAGHRRQFRTAKWRALLAGAIAGPSMLLTGLNTQHTSLAI 664

Query: 688 YIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIF-RSAYVL 747
           YI MRAAVLA+RCGIKSKRFG ICKPLTW++      C ++       R+I F RSAY+L
Sbjct: 665 YILMRAAVLASRCGIKSKRFGGICKPLTWAHD-----CTTTEG-----RQIPFLRSAYIL 724

Query: 748 KQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQM 807
           KQ+SLPPS+ SFLN HGGKDTVIL+G+K    G+P +N  +AIEK+Y+++G ++KLD  M
Sbjct: 725 KQESLPPSYKSFLNKHGGKDTVILQGVKEIARGIPFTN-LEAIEKHYKSIGTNIKLDPGM 784

Query: 808 KTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGL 867
           + PC+I+HG+QSC GHVL+FLIQ YKRALPVYLPVYL+PALIVHR+ L+ R Y IL +GL
Sbjct: 785 QVPCSIVHGDQSCSGHVLSFLIQAYKRALPVYLPVYLIPALIVHRQDLLKRHYSILLKGL 844

Query: 868 LGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSRRIEISL 927
           LGTARSSLFLSVYCSSAW WTC   R  ++ NIP+VA+ TF  GLALAIEKKSRRIEISL
Sbjct: 845 LGTARSSLFLSVYCSSAWAWTCFLFRICRRCNIPMVAMGTFPTGLALAIEKKSRRIEISL 904

Query: 928 YCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRSKYLNVL 961
           YC AR IESFF+CM D+GYLP S   KRADV+IFS+STAIIMHCYAQEREVFRSKYLNVL
Sbjct: 905 YCFARAIESFFTCMADIGYLPPSTKLKRADVVIFSLSTAIIMHCYAQEREVFRSKYLNVL 934

BLAST of Carg20540 vs. NCBI nr
Match: RXH92749.1 (hypothetical protein DVH24_042523 [Malus domestica])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 606/965 (62.80%), Postives = 712/965 (73.78%), Query Frame = 0

Query: 28   NSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEESGSVSVSASFCHQEQPVFFGVLRWRRK 87
            N   F+TGGLF++  VP+S  NS+ S   +   S  VS S            G+    R 
Sbjct: 69   NPTLFITGGLFLEPTVPNSLLNSVVSFQKNSRSSAVVSGS------------GLSYGCRL 128

Query: 88   PRIADCGFLSLSVRGDRFVPEQK--VRADGYENCEKAGKCEGALATVENDKQEKKYTVHK 147
              +A  GF S+S+  D  V E K  +  +G E  E+A + E A+     +++ +K  V  
Sbjct: 129  RAVAGGGFFSVSLSSDGVVRESKGCLVQNGDEGSEEA-EVEAAV----TERKREKVGVRS 188

Query: 148  RNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFYVIKKIAASQGLKGFW 207
              AMNTTKHLW+GAIAAMVSRTFVAPLERLKLEYIVRGEQ +LF +++ IAA+QGL+GFW
Sbjct: 189  GGAMNTTKHLWAGAIAAMVSRTFVAPLERLKLEYIVRGEQRHLFELVRTIAANQGLRGFW 248

Query: 208  KGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVAGAAAGITATVLCLPL 267
            KGN VNILRTAPFKA+NFYAYDTYRKQLL  SG + T+NFERFVAGAAAGITAT+LCLPL
Sbjct: 249  KGNLVNILRTAPFKAINFYAYDTYRKQLLCFSGNKETTNFERFVAGAAAGITATILCLPL 308

Query: 268  DTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSMAPSGAVFYTVYDILK 327
            DTIRT++VAPGGEALGGVIGAFR+M+QTEGFFSLYKGL+PSI SMAPSGAVFY VYDILK
Sbjct: 309  DTIRTQLVAPGGEALGGVIGAFRHMIQTEGFFSLYKGLVPSIASMAPSGAVFYGVYDILK 368

Query: 328  TAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGACAEAATYPFEVIRRQ 387
            +AYL SP+GRKRI  M+QQG+  +ALDQLELGP+RTLLYGAISGACAEAATYPFEV+RRQ
Sbjct: 369  SAYLHSPDGRKRIHKMSQQGQGWSALDQLELGPMRTLLYGAISGACAEAATYPFEVVRRQ 428

Query: 388  LQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPSLLQ----ACDSDLSFTFLSFILSRL 447
            LQ+QVQ TRMSA AT  KIV+ GG+ ALYAGL+PSLLQ    A  S   + F+  +L   
Sbjct: 429  LQLQVQETRMSALATGIKIVEHGGVPALYAGLIPSLLQVLPSAAISYFVYEFMKIVLKFK 488

Query: 448  FNRNSQFSAAV--------AVMSPGGCACIAIENGDAEASGNCKSGGSYCEHCGCGCGS- 507
              + S   A++        A+ SP              A          C        S 
Sbjct: 489  PPKLSDACASLARPVHYRKALPSPSSTPNRLRLRSPIPAFKALTRSPRCCRTATSALASP 548

Query: 508  -------GSADYSSLPAFSCSSYSLWLD------SARLRESGKFWRILVASAKGFTIGAG 567
                    +A    +P    S  SL+        SA L +S K  R++ AS KGFTIGAG
Sbjct: 549  EEMVAPMATATDPPIPTSDPSPSSLYYSSSLGYVSAPLSDSEKLRRVVTASVKGFTIGAG 608

Query: 568  LKGGLSLFSILAGLKRRKALASLGKKGVITNRDAISMALKETLRYGLFLGTFAGTFVSID 627
            LKGGL++FSILA L+RRK LASL  +GVITNR+AI  ALKETLRYGLFLGTFAGTFVS+D
Sbjct: 609  LKGGLAIFSILARLRRRKLLASLRTEGVITNREAIVTALKETLRYGLFLGTFAGTFVSVD 668

Query: 628  EIIGNLAGHR---RTARWRALSAGALAGPSMLLTGLNTQHKTLAIYIFMRAAVLAARCGI 687
            EIIG+LAGHR   RTA+WRAL AGA+AGPSMLLTGLNTQH +LAIYI MRAAVLA+RCGI
Sbjct: 669  EIIGSLAGHRRQFRTAKWRALLAGAIAGPSMLLTGLNTQHTSLAIYILMRAAVLASRCGI 728

Query: 688  KSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIF-RSAYVLKQDSLPPSFSSFLNI 747
            KSKRFG ICKPLTW++      C ++ +     R+I F RSAY+LKQ+SLPPS+ SFLN 
Sbjct: 729  KSKRFGSICKPLTWAHD-----CTTTKR-----RKISFLRSAYILKQESLPPSYKSFLNK 788

Query: 748  HGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQMKTPCTIIHGNQSCGG 807
            HGGKDTVIL+G+K    G+P +N  +AIEK+Y+++G ++KLD  M+ PC+I+HG+QSC G
Sbjct: 789  HGGKDTVILQGVKEIARGIPFTN-LEAIEKHYKSIGKNIKLDPGMQVPCSIVHGDQSCSG 848

Query: 808  HVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGLLGTARSSLFLSVYCS 867
            HVL+FLIQ YKRALPVYLPVYL+PALIVHR+ L+ R Y IL +GLLGTARSSLFLSVYCS
Sbjct: 849  HVLSFLIQAYKRALPVYLPVYLIPALIVHRQDLLKRHYSILLKGLLGTARSSLFLSVYCS 908

Query: 868  SAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSRRIEISLYCLARGIESFFSCMT 927
            SAW WTC   R F++ NIP+VA+ TF  GLALAIEKKSRRIEISLYC AR IESFF+CM 
Sbjct: 909  SAWAWTCFLFRIFRRCNIPMVAMGTFPTGLALAIEKKSRRIEISLYCFARAIESFFTCMA 968

Query: 928  DLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRSKYLNVLDWVFGVPPPPPCDET 961
            D GYLP S   KRADV+IFS+STAIIMHCYAQEREVFRSKYLNVLDWVFGV PPP C ET
Sbjct: 969  DTGYLPPSTKLKRADVVIFSLSTAIIMHCYAQEREVFRSKYLNVLDWVFGV-PPPSC-ET 1003

BLAST of Carg20540 vs. NCBI nr
Match: XP_022952006.1 (uncharacterized protein LOC111454723 isoform X1 [Cucurbita moschata])

HSP 1 Score: 996.9 bits (2576), Expect = 1.2e-286
Identity = 499/510 (97.84%), Postives = 500/510 (98.04%), Query Frame = 0

Query: 455 MSPGGCACIAIENGDAEASGNCKSGGSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS 514
           MSPGGCAC+AIENGDAEASGNCKSG SYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS
Sbjct: 1   MSPGGCACVAIENGDAEASGNCKSGDSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS 60

Query: 515 ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI 574
           ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI
Sbjct: 61  ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI 120

Query: 575 SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT 634
           SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT
Sbjct: 121 SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT 180

Query: 635 QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIF 694
           QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQIL        
Sbjct: 181 QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQIL-------- 240

Query: 695 RSAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV 754
            SAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV
Sbjct: 241 -SAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV 300

Query: 755 KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE 814
           KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE
Sbjct: 301 KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE 360

Query: 815 ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR 874
           ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR
Sbjct: 361 ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR 420

Query: 875 RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS 934
           RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS
Sbjct: 421 RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS 480

Query: 935 KYLNVLDWVFGVPPPPPCDETPSCKNCKQH 965
           KYLNVLDWVFGVPPPPPCDETPSCKNCKQH
Sbjct: 481 KYLNVLDWVFGVPPPPPCDETPSCKNCKQH 501

BLAST of Carg20540 vs. NCBI nr
Match: XP_023538421.1 (uncharacterized protein LOC111799208 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 993.8 bits (2568), Expect = 1.0e-285
Identity = 497/510 (97.45%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 455 MSPGGCACIAIENGDAEASGNCKSGGSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS 514
           MSPGGCAC+AIENGDAEASGNCKSG SYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS
Sbjct: 1   MSPGGCACVAIENGDAEASGNCKSGDSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS 60

Query: 515 ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI 574
           ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI
Sbjct: 61  ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI 120

Query: 575 SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT 634
           SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTA WRALSAGALAGPSMLLTGLNT
Sbjct: 121 SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTAGWRALSAGALAGPSMLLTGLNT 180

Query: 635 QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIF 694
           QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQIL        
Sbjct: 181 QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQIL-------- 240

Query: 695 RSAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV 754
            SAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKA+EKYYRAMGADV
Sbjct: 241 -SAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAVEKYYRAMGADV 300

Query: 755 KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE 814
           KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE
Sbjct: 301 KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE 360

Query: 815 ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR 874
           ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR
Sbjct: 361 ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR 420

Query: 875 RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS 934
           RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS
Sbjct: 421 RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS 480

Query: 935 KYLNVLDWVFGVPPPPPCDETPSCKNCKQH 965
           KYLNVLDWVFGVPPPPPCDETPSCKNCKQH
Sbjct: 481 KYLNVLDWVFGVPPPPPCDETPSCKNCKQH 501

BLAST of Carg20540 vs. ExPASy Swiss-Prot
Match: Q9LV81 (Probable mitochondrial adenine nucleotide transporter BTL3 OS=Arabidopsis thaliana OX=3702 GN=At5g64970 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.0e-131
Identity = 243/402 (60.45%), Postives = 315/402 (78.36%), Query Frame = 0

Query: 28  NSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEESGSVSVSASFCHQEQPVFFGVLRWRRK 87
           ++G  + GGLF++ ++PSS  + + SK+ SV            C   Q   F  L++RR+
Sbjct: 19  HNGQIICGGLFLEESLPSSSVSFLSSKDCSVNS----------CRFSQKSSF--LKFRRR 78

Query: 88  PRIADCGFLSLSVRGDRFVPEQKVRADGY--ENCEKAGKCEGALATVENDKQEKKYTVHK 147
               +  FLS+S+  +    E++   +GY  +N  K+ K    +   +  K++++   + 
Sbjct: 79  NGTREPLFLSVSLSINESNGEEE-EGEGYNGQNGFKSEKGSVLIGGGQESKEKRRVKENG 138

Query: 148 RNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFYVIKKIAASQGLKGFW 207
             A+NTTKHLW+GA AAMVSRT +APLER+KLEYIVRGEQGNL  +I++IA ++G++GFW
Sbjct: 139 AGALNTTKHLWAGAFAAMVSRTCIAPLERMKLEYIVRGEQGNLLELIQRIATNEGIRGFW 198

Query: 208 KGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVAGAAAGITATVLCLPL 267
           KGN VNILRTAPFK++NFYAYDTYR QLLKLSG E T+NFERFVAGAAAG+TA++LCLPL
Sbjct: 199 KGNLVNILRTAPFKSINFYAYDTYRGQLLKLSGNEETTNFERFVAGAAAGVTASLLCLPL 258

Query: 268 DTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSMAPSGAVFYTVYDILK 327
           DTIRT +VAPGGEALGGV+GAFR+M+QTEGFFSLYKGL+PS++SMAPSGAVFY VYDILK
Sbjct: 259 DTIRTVMVAPGGEALGGVVGAFRHMIQTEGFFSLYKGLVPSLVSMAPSGAVFYGVYDILK 318

Query: 328 TAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGACAEAATYPFEVIRRQ 387
           +AYL +PEG+KR+++M Q+G++LNA DQLELGP+RTLLYGAI+GAC+EAATYPFEV+RR+
Sbjct: 319 SAYLHTPEGKKRLEHMKQEGEELNAFDQLELGPMRTLLYGAIAGACSEAATYPFEVVRRR 378

Query: 388 LQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPSLLQACDS 428
           LQMQ  A R+SA ATC KI++QGG+ ALYAGL+PSLLQ   S
Sbjct: 379 LQMQSHAKRLSAVATCVKIIEQGGVPALYAGLIPSLLQVLPS 407

BLAST of Carg20540 vs. ExPASy Swiss-Prot
Match: Q9C9R4 (Probable mitochondrial adenine nucleotide transporter BTL2 OS=Arabidopsis thaliana OX=3702 GN=At1g78180 PE=2 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.2e-124
Identity = 251/417 (60.19%), Postives = 302/417 (72.42%), Query Frame = 0

Query: 16  PSQSQSLSCCS-TNSGFFVTGGLFID-SAVPSSFANSIWSKNSSVEESGSVSVSASFCHQ 75
           PS S S S    +N  FF TGGLF++   V SSF +SI SK                C  
Sbjct: 11  PSSSSSTSSIDLSNEAFFSTGGLFLEPPGVSSSFFDSISSK----------------CSD 70

Query: 76  EQPVFFGVLRWRRKPRI---ADCGFLSLSVRGDRFVPEQKVRADGYENCEKAGKCEGALA 135
            +P+ F    WR K R+    +  FLS+S+  DR   EQ+ +    +N E  GK      
Sbjct: 71  SEPLHFPGY-WRNKTRLRSGKNFMFLSVSLSKDR--SEQQCKKALAQNDEIPGK------ 130

Query: 136 TVENDKQEKKYTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFY 195
             +N K+     V +R  MNT KHLW+GA+AAMVS+TF+APLERLKLEY VRGEQ NL  
Sbjct: 131 --DNRKRSVIGGVRRRGTMNTRKHLWAGAVAAMVSKTFLAPLERLKLEYTVRGEQRNLLV 190

Query: 196 VIKKIAASQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVA 255
           V K IA +QGL GFWKGN +N+LRTAPFKA+NF AYDTYRKQLLK++G +  +NFERFVA
Sbjct: 191 VAKSIATTQGLTGFWKGNLLNVLRTAPFKAVNFCAYDTYRKQLLKIAGNQEATNFERFVA 250

Query: 256 GAAAGITATVLCLPLDTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSM 315
           GAAAGITATVLCLPLDTIRTK+VA GGEALGG+ GAFRYM+QTEG FSLYKGL+PSI SM
Sbjct: 251 GAAAGITATVLCLPLDTIRTKLVARGGEALGGIGGAFRYMIQTEGLFSLYKGLVPSIASM 310

Query: 316 APSGAVFYTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGA 375
           A SGAVFY VYDILK+++L +PEGRKR+ +M QQG++LNALD+LELGPIRTL+YGAI+GA
Sbjct: 311 ALSGAVFYGVYDILKSSFLHTPEGRKRLIDMKQQGQELNALDRLELGPIRTLMYGAIAGA 370

Query: 376 CAEAATYPFEVIRRQLQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPSLLQACDS 428
           C E ATYPFEV+RRQLQMQ+   +++A A    I+++GGI ALYAGLLPSLLQ   S
Sbjct: 371 CTEVATYPFEVVRRQLQMQMGKNKLNALAMGFNIIERGGIPALYAGLLPSLLQVLPS 400

BLAST of Carg20540 vs. ExPASy Swiss-Prot
Match: Q7ZY36 (Calcium-binding mitochondrial carrier protein SCaMC-1-A OS=Xenopus laevis OX=8355 GN=slc25a24-a PE=2 SV=2)

HSP 1 Score: 149.8 bits (377), Expect = 1.6e-34
Identity = 89/289 (30.80%), Postives = 149/289 (51.56%), Query Frame = 0

Query: 140 KYTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQG--NLFYVIKKIAA 199
           ++T  ++      KHL +G +A  VSRT  APL+RLK+   V G +G  N+   +K++  
Sbjct: 183 EFTEEEKKTGQWWKHLLAGGMAGAVSRTGTAPLDRLKVMMQVHGTKGNSNIITGLKQMVK 242

Query: 200 SQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVAGAAAGIT 259
             G++  W+GN VN+++ AP  A+ F+AY+ Y+K     SG+  T+  ERF+AG+ AG T
Sbjct: 243 EGGVRSLWRGNGVNVIKIAPETAMKFWAYEQYKKLFTSESGKLGTA--ERFIAGSLAGAT 302

Query: 260 ATVLCLPLDTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSMAPSGAVF 319
           A     P++ ++T++         G+    + ++Q EG  + YKG +P+IL + P   + 
Sbjct: 303 AQTSIYPMEVLKTRLAVGKTGQYSGMFDCAKKIMQKEGILAFYKGYIPNILGIIPYAGID 362

Query: 320 YTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGACAEAATY 379
             +Y+ LK  +LQ+                  A D    G +  L  G +S  C + A+Y
Sbjct: 363 LAIYETLKNYWLQN-----------------YAKDSANPGVLVLLGCGTVSSTCGQLASY 422

Query: 380 PFEVIRRQLQMQVQ---ATRMSAFATCSKIVQQGGIQALYAGLLPSLLQ 424
           P  +IR ++Q Q     A +++      KIV + G   LY G+ P+ L+
Sbjct: 423 PLALIRTRMQAQASIEGAPQLNMGGLFRKIVAKEGFFGLYTGIAPNFLK 452

BLAST of Carg20540 vs. ExPASy Swiss-Prot
Match: Q54MZ4 (Mitochondrial substrate carrier family protein B OS=Dictyostelium discoideum OX=44689 GN=mcfB PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 5.5e-32
Identity = 95/303 (31.35%), Postives = 149/303 (49.17%), Query Frame = 0

Query: 140 KYTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKL--------------EYIVRGEQ 199
           K  VH    + + K L SG +A  VSRT  +PLERLK+              +Y  RG  
Sbjct: 127 KPIVHAPADVPSWKLLLSGGVAGAVSRTCTSPLERLKILNQVGHMNLEQNAPKYKGRG-- 186

Query: 200 GNLFYVIKKIAASQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNF 259
             +   +K +  ++G  GF+KGN  N++R AP+ A+ F +Y+ Y+  LL  + +   + +
Sbjct: 187 --IIQSLKTMYTTEGFIGFFKGNGTNVIRIAPYSAIQFLSYEKYKNFLLNNNDQTHLTTY 246

Query: 260 ERFVAGAAAGITATVLCLPLDTIRTKI-VAPGGEALGGVIGAFRYMVQTEGFFSLYKGLL 319
           E    G AAG+T+ +   PLD IR+++ V   G    G+    + +++ EG   LYKGL 
Sbjct: 247 ENLFVGGAAGVTSLLCTYPLDLIRSRLTVQVFGNKYNGIADTCKMIIREEGVAGLYKGLF 306

Query: 320 PSILSMAPSGAVFYTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLY 379
            S L +AP  A+ +T Y+ LK  ++        +Q                     +L +
Sbjct: 307 ASALGVAPYVAINFTTYENLKKTFIPKDTTPTVVQ---------------------SLTF 366

Query: 380 GAISGACAEAATYPFEVIRRQLQMQ----VQATRMSAFATCSKIVQQGGIQALYAGLLPS 424
           GAISGA A+  TYP ++IRR+LQ+Q            F    KI++  G+  LY G++P 
Sbjct: 367 GAISGATAQTLTYPIDLIRRRLQVQGIGGKDILYNGTFDAFRKIIRDEGVLGLYNGMIPC 404

BLAST of Carg20540 vs. ExPASy Swiss-Prot
Match: Q9SUV1 (Adenine nucleotide transporter BT1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BT1 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.2e-31
Identity = 95/297 (31.99%), Postives = 156/297 (52.53%), Query Frame = 0

Query: 133 ENDKQEKK--YTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFY 192
           E  K++KK   T+  + A  + + L SGA+A  VSRT VAPLE ++   +V     +   
Sbjct: 90  EKRKRKKKGGLTLKIKIANPSLRRLLSGAVAGAVSRTVVAPLETIRTHLMVGSGGNSSTE 149

Query: 193 VIKKIAASQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNF-ERFV 252
           V   I   +G  G ++GN VN++R AP +A+  + ++T  K+L    G+E+        +
Sbjct: 150 VFSDIMKHEGWTGLFRGNLVNVIRVAPARAVELFVFETVNKKLSPPHGQESKIPIPASLL 209

Query: 253 AGAAAGITATVLCLPLDTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILS 312
           AGA AG++ T+L  PL+ ++T++    G    G+  AF  +++ EG   LY+GL PS++ 
Sbjct: 210 AGACAGVSQTLLTYPLELVKTRLTIQRG-VYKGIFDAFLKIIREEGPTELYRGLAPSLIG 269

Query: 313 MAPSGAVFYTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISG 372
           + P  A  Y  YD L+ AY          ++ ++Q K         +G I TLL G+++G
Sbjct: 270 VVPYAATNYFAYDSLRKAY----------RSFSKQEK---------IGNIETLLIGSLAG 329

Query: 373 ACAEAATYPFEVIRRQLQMQVQATRM---SAFATCSKIVQQGGIQALYAGLLPSLLQ 424
           A +  AT+P EV R+ +Q+   + R+   +       I++  GI   Y GL PS L+
Sbjct: 330 ALSSTATFPLEVARKHMQVGAVSGRVVYKNMLHALVTILEHEGILGWYKGLGPSCLK 366

BLAST of Carg20540 vs. ExPASy TrEMBL
Match: A0A5N5HM10 (TMEM135_C_rich domain-containing protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_033764 PE=3 SV=1)

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 611/980 (62.35%), Postives = 712/980 (72.65%), Query Frame = 0

Query: 28  NSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEESGSVSVSASFCHQEQPVFFGVLRWRRK 87
           N   F+TGGLF++  VP+S  NS+ S   +   +  VS                      
Sbjct: 5   NPTLFITGGLFLEPTVPNSLLNSVVSFQKNSPSAAVVSGGGG------------------ 64

Query: 88  PRIADCGFLSLSVRGDRFVPEQK--VRADGYENCEKAGKCEGALATVENDKQEKKYTVHK 147
                 GF S+S+  D  V E K  +  +G E  E     E     V  +++ +K  V  
Sbjct: 65  -----GGFFSVSLSSDGVVRESKGCLVHNGDEGSE-----EAEAEAVVTERKREKVGVRS 124

Query: 148 RNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFYVIKKIAASQGLKGFW 207
             AMNTTKHLW+GAIAAMVSRTFVAPLERLKLEYIVRGEQ +LF +++ IAA+QGL+GFW
Sbjct: 125 GGAMNTTKHLWAGAIAAMVSRTFVAPLERLKLEYIVRGEQRHLFELVRTIAANQGLRGFW 184

Query: 208 KGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVAGAAAGITATVLCLPL 267
           KGN VNILRTAPFKA+NFYAYDTYRKQLL++SG + T+NFERFVAGAAAGITAT+LCLPL
Sbjct: 185 KGNLVNILRTAPFKAINFYAYDTYRKQLLRVSGNKETTNFERFVAGAAAGITATILCLPL 244

Query: 268 DTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSMAPSGAVFYTVYDILK 327
           DTIRT++VAPGGEALGGVIGAFR+M+QTEGFFSLYKGL+PSI SMAPSGAVFY VYDILK
Sbjct: 245 DTIRTQLVAPGGEALGGVIGAFRHMIQTEGFFSLYKGLVPSIASMAPSGAVFYGVYDILK 304

Query: 328 TAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGACAEAATYPFEVIRRQ 387
           +AYL SP+GRKRI  M+QQG+  +ALDQLELGPIRTLLYGAISGACAEAATYPFEV+RRQ
Sbjct: 305 SAYLHSPDGRKRIHKMSQQGQGRSALDQLELGPIRTLLYGAISGACAEAATYPFEVVRRQ 364

Query: 388 LQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPSLLQ---------------------- 447
           LQ+QVQ TRMSA AT  KIV+ GG+ ALYAGL+PSLLQ                      
Sbjct: 365 LQLQVQETRMSALATGLKIVEHGGVPALYAGLIPSLLQVLPSAAISYFVYEFMKIVLKFK 424

Query: 448 ------ACDS---------DLSFTFLSFILSRLFNRNS----QFSAAVAVMSPGGCACIA 507
                 AC S          L F+FLS+  S     +S     +S A  +     C C A
Sbjct: 425 PPKLSDACASLARPVDYRKALPFSFLSYEPSSASQPHSCMQGPYSFAAMLPDRDLCTCFA 484

Query: 508 IENGDAEASGNCKSGGSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDSARLRESGKFW 567
            ENG  +   +  S    C HC     S S+ Y         S SL   SA L +S K  
Sbjct: 485 GENGGPDGDRH-GSADPNCTHCRKSDPSPSSLY--------YSSSLGYVSAPLSDSEKLR 544

Query: 568 RILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAISMALKETLRY 627
           R++ AS KGFTIGAGLKGGL++FSILA L+RRK LASL  +GVITNR+AI  ALKETLRY
Sbjct: 545 RVVTASVKGFTIGAGLKGGLAIFSILARLRRRKLLASLRTEGVITNREAIVTALKETLRY 604

Query: 628 GLFLGTFAGTFVSIDEIIGNLAGHR---RTARWRALSAGALAGPSMLLTGLNTQHKTLAI 687
           GLFLGTFAGTFVS+DEIIG+LAGHR   RTA+WRAL AGA+AGPSMLLTGLNTQH +LAI
Sbjct: 605 GLFLGTFAGTFVSVDEIIGSLAGHRRQFRTAKWRALLAGAIAGPSMLLTGLNTQHTSLAI 664

Query: 688 YIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIF-RSAYVL 747
           YI MRAAVLA+RCGIKSKRFG ICKPLTW++      C ++       R+I F RSAY+L
Sbjct: 665 YILMRAAVLASRCGIKSKRFGGICKPLTWAHD-----CTTTEG-----RQIPFLRSAYIL 724

Query: 748 KQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQM 807
           KQ+SLPPS+ SFLN HGGKDTVIL+G+K    G+P +N  +AIEK+Y+++G ++KLD  M
Sbjct: 725 KQESLPPSYKSFLNKHGGKDTVILQGVKEIARGIPFTN-LEAIEKHYKSIGTNIKLDPGM 784

Query: 808 KTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGL 867
           + PC+I+HG+QSC GHVL+FLIQ YKRALPVYLPVYL+PALIVHR+ L+ R Y IL +GL
Sbjct: 785 QVPCSIVHGDQSCSGHVLSFLIQAYKRALPVYLPVYLIPALIVHRQDLLKRHYSILLKGL 844

Query: 868 LGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSRRIEISL 927
           LGTARSSLFLSVYCSSAW WTC   R  ++ NIP+VA+ TF  GLALAIEKKSRRIEISL
Sbjct: 845 LGTARSSLFLSVYCSSAWAWTCFLFRICRRCNIPMVAMGTFPTGLALAIEKKSRRIEISL 904

Query: 928 YCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRSKYLNVL 961
           YC AR IESFF+CM D+GYLP S   KRADV+IFS+STAIIMHCYAQEREVFRSKYLNVL
Sbjct: 905 YCFARAIESFFTCMADIGYLPPSTKLKRADVVIFSLSTAIIMHCYAQEREVFRSKYLNVL 934

BLAST of Carg20540 vs. ExPASy TrEMBL
Match: A0A498JAB5 (TMEM135_C_rich domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_042523 PE=3 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 606/965 (62.80%), Postives = 712/965 (73.78%), Query Frame = 0

Query: 28   NSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEESGSVSVSASFCHQEQPVFFGVLRWRRK 87
            N   F+TGGLF++  VP+S  NS+ S   +   S  VS S            G+    R 
Sbjct: 69   NPTLFITGGLFLEPTVPNSLLNSVVSFQKNSRSSAVVSGS------------GLSYGCRL 128

Query: 88   PRIADCGFLSLSVRGDRFVPEQK--VRADGYENCEKAGKCEGALATVENDKQEKKYTVHK 147
              +A  GF S+S+  D  V E K  +  +G E  E+A + E A+     +++ +K  V  
Sbjct: 129  RAVAGGGFFSVSLSSDGVVRESKGCLVQNGDEGSEEA-EVEAAV----TERKREKVGVRS 188

Query: 148  RNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFYVIKKIAASQGLKGFW 207
              AMNTTKHLW+GAIAAMVSRTFVAPLERLKLEYIVRGEQ +LF +++ IAA+QGL+GFW
Sbjct: 189  GGAMNTTKHLWAGAIAAMVSRTFVAPLERLKLEYIVRGEQRHLFELVRTIAANQGLRGFW 248

Query: 208  KGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVAGAAAGITATVLCLPL 267
            KGN VNILRTAPFKA+NFYAYDTYRKQLL  SG + T+NFERFVAGAAAGITAT+LCLPL
Sbjct: 249  KGNLVNILRTAPFKAINFYAYDTYRKQLLCFSGNKETTNFERFVAGAAAGITATILCLPL 308

Query: 268  DTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSMAPSGAVFYTVYDILK 327
            DTIRT++VAPGGEALGGVIGAFR+M+QTEGFFSLYKGL+PSI SMAPSGAVFY VYDILK
Sbjct: 309  DTIRTQLVAPGGEALGGVIGAFRHMIQTEGFFSLYKGLVPSIASMAPSGAVFYGVYDILK 368

Query: 328  TAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGACAEAATYPFEVIRRQ 387
            +AYL SP+GRKRI  M+QQG+  +ALDQLELGP+RTLLYGAISGACAEAATYPFEV+RRQ
Sbjct: 369  SAYLHSPDGRKRIHKMSQQGQGWSALDQLELGPMRTLLYGAISGACAEAATYPFEVVRRQ 428

Query: 388  LQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPSLLQ----ACDSDLSFTFLSFILSRL 447
            LQ+QVQ TRMSA AT  KIV+ GG+ ALYAGL+PSLLQ    A  S   + F+  +L   
Sbjct: 429  LQLQVQETRMSALATGIKIVEHGGVPALYAGLIPSLLQVLPSAAISYFVYEFMKIVLKFK 488

Query: 448  FNRNSQFSAAV--------AVMSPGGCACIAIENGDAEASGNCKSGGSYCEHCGCGCGS- 507
              + S   A++        A+ SP              A          C        S 
Sbjct: 489  PPKLSDACASLARPVHYRKALPSPSSTPNRLRLRSPIPAFKALTRSPRCCRTATSALASP 548

Query: 508  -------GSADYSSLPAFSCSSYSLWLD------SARLRESGKFWRILVASAKGFTIGAG 567
                    +A    +P    S  SL+        SA L +S K  R++ AS KGFTIGAG
Sbjct: 549  EEMVAPMATATDPPIPTSDPSPSSLYYSSSLGYVSAPLSDSEKLRRVVTASVKGFTIGAG 608

Query: 568  LKGGLSLFSILAGLKRRKALASLGKKGVITNRDAISMALKETLRYGLFLGTFAGTFVSID 627
            LKGGL++FSILA L+RRK LASL  +GVITNR+AI  ALKETLRYGLFLGTFAGTFVS+D
Sbjct: 609  LKGGLAIFSILARLRRRKLLASLRTEGVITNREAIVTALKETLRYGLFLGTFAGTFVSVD 668

Query: 628  EIIGNLAGHR---RTARWRALSAGALAGPSMLLTGLNTQHKTLAIYIFMRAAVLAARCGI 687
            EIIG+LAGHR   RTA+WRAL AGA+AGPSMLLTGLNTQH +LAIYI MRAAVLA+RCGI
Sbjct: 669  EIIGSLAGHRRQFRTAKWRALLAGAIAGPSMLLTGLNTQHTSLAIYILMRAAVLASRCGI 728

Query: 688  KSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIF-RSAYVLKQDSLPPSFSSFLNI 747
            KSKRFG ICKPLTW++      C ++ +     R+I F RSAY+LKQ+SLPPS+ SFLN 
Sbjct: 729  KSKRFGSICKPLTWAHD-----CTTTKR-----RKISFLRSAYILKQESLPPSYKSFLNK 788

Query: 748  HGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQMKTPCTIIHGNQSCGG 807
            HGGKDTVIL+G+K    G+P +N  +AIEK+Y+++G ++KLD  M+ PC+I+HG+QSC G
Sbjct: 789  HGGKDTVILQGVKEIARGIPFTN-LEAIEKHYKSIGKNIKLDPGMQVPCSIVHGDQSCSG 848

Query: 808  HVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGLLGTARSSLFLSVYCS 867
            HVL+FLIQ YKRALPVYLPVYL+PALIVHR+ L+ R Y IL +GLLGTARSSLFLSVYCS
Sbjct: 849  HVLSFLIQAYKRALPVYLPVYLIPALIVHRQDLLKRHYSILLKGLLGTARSSLFLSVYCS 908

Query: 868  SAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSRRIEISLYCLARGIESFFSCMT 927
            SAW WTC   R F++ NIP+VA+ TF  GLALAIEKKSRRIEISLYC AR IESFF+CM 
Sbjct: 909  SAWAWTCFLFRIFRRCNIPMVAMGTFPTGLALAIEKKSRRIEISLYCFARAIESFFTCMA 968

Query: 928  DLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRSKYLNVLDWVFGVPPPPPCDET 961
            D GYLP S   KRADV+IFS+STAIIMHCYAQEREVFRSKYLNVLDWVFGV PPP C ET
Sbjct: 969  DTGYLPPSTKLKRADVVIFSLSTAIIMHCYAQEREVFRSKYLNVLDWVFGV-PPPSC-ET 1003

BLAST of Carg20540 vs. ExPASy TrEMBL
Match: A0A6J1GJ26 (uncharacterized protein LOC111454723 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454723 PE=4 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 5.9e-287
Identity = 499/510 (97.84%), Postives = 500/510 (98.04%), Query Frame = 0

Query: 455 MSPGGCACIAIENGDAEASGNCKSGGSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS 514
           MSPGGCAC+AIENGDAEASGNCKSG SYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS
Sbjct: 1   MSPGGCACVAIENGDAEASGNCKSGDSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS 60

Query: 515 ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI 574
           ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI
Sbjct: 61  ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI 120

Query: 575 SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT 634
           SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT
Sbjct: 121 SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT 180

Query: 635 QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIF 694
           QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQIL        
Sbjct: 181 QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQIL-------- 240

Query: 695 RSAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV 754
            SAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV
Sbjct: 241 -SAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV 300

Query: 755 KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE 814
           KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE
Sbjct: 301 KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE 360

Query: 815 ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR 874
           ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR
Sbjct: 361 ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR 420

Query: 875 RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS 934
           RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS
Sbjct: 421 RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS 480

Query: 935 KYLNVLDWVFGVPPPPPCDETPSCKNCKQH 965
           KYLNVLDWVFGVPPPPPCDETPSCKNCKQH
Sbjct: 481 KYLNVLDWVFGVPPPPPCDETPSCKNCKQH 501

BLAST of Carg20540 vs. ExPASy TrEMBL
Match: A0A6J1KK52 (uncharacterized protein LOC111496453 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496453 PE=4 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 8.2e-281
Identity = 493/510 (96.67%), Postives = 494/510 (96.86%), Query Frame = 0

Query: 455 MSPGGCACIAIENGDAEASGNCKSGGSYCEHCGCGCGSGSADYSSLPAFSCSSYSLWLDS 514
           MSPGGC    IENGDAEA+GNCKSG SYCEHCG GCGSGSADYSSLPAFSCSSYSLWLDS
Sbjct: 1   MSPGGC----IENGDAEAAGNCKSGDSYCEHCGRGCGSGSADYSSLPAFSCSSYSLWLDS 60

Query: 515 ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDAI 574
           ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRD I
Sbjct: 61  ARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVITNRDVI 120

Query: 575 SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT 634
           SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT
Sbjct: 121 SMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNT 180

Query: 635 QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIF 694
           QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQIL        
Sbjct: 181 QHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQIL-------- 240

Query: 695 RSAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV 754
            SAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV
Sbjct: 241 -SAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADV 300

Query: 755 KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE 814
           KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE
Sbjct: 301 KLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYE 360

Query: 815 ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR 874
           ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR
Sbjct: 361 ILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSR 420

Query: 875 RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS 934
           RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS
Sbjct: 421 RIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRS 480

Query: 935 KYLNVLDWVFGVPPPPPCDETPSCKNCKQH 965
           KYLNVLDWVFGVPPPPPCDETPSCKNCKQH
Sbjct: 481 KYLNVLDWVFGVPPPPPCDETPSCKNCKQH 497

BLAST of Carg20540 vs. ExPASy TrEMBL
Match: A0A6J1BRD3 (uncharacterized protein LOC111004892 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004892 PE=4 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 2.9e-254
Identity = 452/516 (87.60%), Postives = 470/516 (91.09%), Query Frame = 0

Query: 449 SAAVAVMSPGGCACIAIENGDAEASGNCKSGGSYCEHCGCGCGSGSADYSSLPAFSCSSY 508
           SA     + GGCAC+A +NGD E++GNCKSG SYC+HCGC    GSAD SSLPAFSCSS 
Sbjct: 4   SAVEGFDADGGCACVARQNGDVESAGNCKSGESYCQHCGC----GSADSSSLPAFSCSSS 63

Query: 509 SLWLDSARLRESGKFWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLGKKGVI 568
           SLW DS RLRESGK WRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASL KKGVI
Sbjct: 64  SLWFDSMRLRESGKLWRILVASAKGFTIGAGLKGGLSLFSILAGLKRRKALASLRKKGVI 123

Query: 569 TNRDAISMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALSAGALAGPSML 628
           TNRDAISMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRAL AGALAGPSML
Sbjct: 124 TNRDAISMALKETLRYGLFLGTFAGTFVSIDEIIGNLAGHRRTARWRALLAGALAGPSML 183

Query: 629 LTGLNTQHKTLAIYIFMRAAVLAARCGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQL 688
           LTGLNTQHK+LAIYIFMRAAVLA+RCGIKSK+ GHICKPLTWSYGDIFLMCLSSSQIL  
Sbjct: 184 LTGLNTQHKSLAIYIFMRAAVLASRCGIKSKQLGHICKPLTWSYGDIFLMCLSSSQIL-- 243

Query: 689 IRRIIFRSAYVLKQDSLPPSFSSFLNIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYR 748
                  SAYVLKQDSLPPSF SFLN HGGKDTVILEGLKSFVSGMPSSNKF+ IEKYYR
Sbjct: 244 -------SAYVLKQDSLPPSFRSFLNTHGGKDTVILEGLKSFVSGMPSSNKFEVIEKYYR 303

Query: 749 AMGADVKLDLQMKTPCTIIHGNQSCGGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGL 808
           AMGAD KLDLQMKTPCTIIHGNQSCGGH ++FLIQGYKRALPVYLPVYLVPALIVHR+ L
Sbjct: 304 AMGADTKLDLQMKTPCTIIHGNQSCGGHFISFLIQGYKRALPVYLPVYLVPALIVHRQDL 363

Query: 809 MNRPYEILARGLLGTARSSLFLSVYCSSAWMWTCLTARTFKKINIPLVALATFLAGLALA 868
           +NRP EILARGLLGTARSSLFLS+YCSSAWMWTCLT+RTFKKINIPLVALATFL GLALA
Sbjct: 364 LNRPNEILARGLLGTARSSLFLSLYCSSAWMWTCLTSRTFKKINIPLVALATFLTGLALA 423

Query: 869 IEKKSRRIEISLYCLARGIESFFSCMTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQE 928
           IEKKSRRIEISLYCLARGIESFF C TD GYLPQSLNFKRADVI+FS+STAIIMHCYAQE
Sbjct: 424 IEKKSRRIEISLYCLARGIESFFICATDAGYLPQSLNFKRADVIVFSLSTAIIMHCYAQE 483

Query: 929 REVFRSKYLNVLDWVFGVPPPPPCDETPSCKNCKQH 965
           REVFRSKYLNVLDWVFGV PPPPC ETP C N KQH
Sbjct: 484 REVFRSKYLNVLDWVFGV-PPPPC-ETPRCTNSKQH 504

BLAST of Carg20540 vs. TAIR 10
Match: AT1G34630.1 (BEST Arabidopsis thaliana protein match is: Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein (TAIR:AT5G51150.1); Has 323 Blast hits to 315 proteins in 124 species: Archae - 0; Bacteria - 0; Metazoa - 95; Fungi - 110; Plants - 73; Viruses - 0; Other Eukaryotes - 45 (source: NCBI BLink). )

HSP 1 Score: 604.7 bits (1558), Expect = 1.3e-172
Identity = 310/486 (63.79%), Postives = 371/486 (76.34%), Query Frame = 0

Query: 478 SGGSYCEHCGCGCGSGSADYSSLPAFS---CSSYSLWLDSARLRESGKFWRILVASAKGF 537
           + GS C  C      G+ D+     F+   C +      S  + +S K  RI+VAS KGF
Sbjct: 8   TNGSRCSSCISSDNDGN-DFGKSDEFTCKQCKNSDSKSKSKSVNDSDKLRRIIVASVKGF 67

Query: 538 TIGAGLKGGLSLFSILAGLKRRKALASLGKK-GVITNRDAISMALKETLRYGLFLGTFAG 597
           TIG GLKGGL++FSI+A   RR+  +   +K G  +N +AI+M +KETLRYGLFLGTFAG
Sbjct: 68  TIGTGLKGGLAIFSIVARFARRRRSSPQSRKTGEFSNSEAIAMGIKETLRYGLFLGTFAG 127

Query: 598 TFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNTQHKTLAIYIFMRAAVLAAR 657
           TFVS+DE I  LAG +RTA+WRAL AG +AGPSMLLTG NTQH +LA+YI MRAAVLA+R
Sbjct: 128 TFVSVDEAIAALAGDKRTAKWRALFAGLVAGPSMLLTGPNTQHTSLAVYILMRAAVLASR 187

Query: 658 CGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIFRSAYVLKQDSLPPSFSSFL 717
           CGIKSKRFG ICKPLTW +GD+FLMCLSSSQIL         SAY+LKQ+SLP S+ SFL
Sbjct: 188 CGIKSKRFGTICKPLTWKHGDLFLMCLSSSQIL---------SAYILKQESLPSSYKSFL 247

Query: 718 NIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQMKTPCTIIHGNQSC 777
           N  GGKD  IL+G+K   +  P +N  +AIEKYY+++G D+KLD  MK PCTIIHGN+SC
Sbjct: 248 NKQGGKDLSILQGVKDIATAQPFTN-LRAIEKYYKSVGVDIKLDPTMKVPCTIIHGNESC 307

Query: 778 GGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGLLGTARSSLFLSVY 837
             H + F +Q YKRALPVY+PVYL+PALIVHR+ L+ + Y IL +GLLGTARSSLFL+ Y
Sbjct: 308 VKHGVTFFLQAYKRALPVYVPVYLIPALIVHRQDLLKKQYSILGKGLLGTARSSLFLATY 367

Query: 838 CSSAWMWTCLTARTFKKINIPLVALATFLAGLALAIEKKSRRIEISLYCLARGIESFFSC 897
           CSSAW WTCL  RTF+  NIPLVA+ATF  GLALAIEKKSRRIEISLYCLAR IESFF+C
Sbjct: 368 CSSAWAWTCLLFRTFETCNIPLVAIATFPTGLALAIEKKSRRIEISLYCLARAIESFFTC 427

Query: 898 MTDLGYLPQSLNFKRADVIIFSISTAIIMHCYAQEREVFRSKYLNVLDWVFGVPPPPPCD 957
           MT+ GY+    + +RADV++FS+STAIIMHCYAQER+VFRSKYLNVLDWVFGVPPPPP  
Sbjct: 428 MTEAGYIRPPKSLRRADVVVFSVSTAIIMHCYAQERDVFRSKYLNVLDWVFGVPPPPP-- 479

Query: 958 ETPSCK 960
             PSC+
Sbjct: 488 -APSCE 479

BLAST of Carg20540 vs. TAIR 10
Match: AT5G64970.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 471.1 bits (1211), Expect = 2.2e-132
Identity = 243/402 (60.45%), Postives = 315/402 (78.36%), Query Frame = 0

Query: 28  NSGFFVTGGLFIDSAVPSSFANSIWSKNSSVEESGSVSVSASFCHQEQPVFFGVLRWRRK 87
           ++G  + GGLF++ ++PSS  + + SK+ SV            C   Q   F  L++RR+
Sbjct: 19  HNGQIICGGLFLEESLPSSSVSFLSSKDCSVNS----------CRFSQKSSF--LKFRRR 78

Query: 88  PRIADCGFLSLSVRGDRFVPEQKVRADGY--ENCEKAGKCEGALATVENDKQEKKYTVHK 147
               +  FLS+S+  +    E++   +GY  +N  K+ K    +   +  K++++   + 
Sbjct: 79  NGTREPLFLSVSLSINESNGEEE-EGEGYNGQNGFKSEKGSVLIGGGQESKEKRRVKENG 138

Query: 148 RNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFYVIKKIAASQGLKGFW 207
             A+NTTKHLW+GA AAMVSRT +APLER+KLEYIVRGEQGNL  +I++IA ++G++GFW
Sbjct: 139 AGALNTTKHLWAGAFAAMVSRTCIAPLERMKLEYIVRGEQGNLLELIQRIATNEGIRGFW 198

Query: 208 KGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVAGAAAGITATVLCLPL 267
           KGN VNILRTAPFK++NFYAYDTYR QLLKLSG E T+NFERFVAGAAAG+TA++LCLPL
Sbjct: 199 KGNLVNILRTAPFKSINFYAYDTYRGQLLKLSGNEETTNFERFVAGAAAGVTASLLCLPL 258

Query: 268 DTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSMAPSGAVFYTVYDILK 327
           DTIRT +VAPGGEALGGV+GAFR+M+QTEGFFSLYKGL+PS++SMAPSGAVFY VYDILK
Sbjct: 259 DTIRTVMVAPGGEALGGVVGAFRHMIQTEGFFSLYKGLVPSLVSMAPSGAVFYGVYDILK 318

Query: 328 TAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGACAEAATYPFEVIRRQ 387
           +AYL +PEG+KR+++M Q+G++LNA DQLELGP+RTLLYGAI+GAC+EAATYPFEV+RR+
Sbjct: 319 SAYLHTPEGKKRLEHMKQEGEELNAFDQLELGPMRTLLYGAIAGACSEAATYPFEVVRRR 378

Query: 388 LQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPSLLQACDS 428
           LQMQ  A R+SA ATC KI++QGG+ ALYAGL+PSLLQ   S
Sbjct: 379 LQMQSHAKRLSAVATCVKIIEQGGVPALYAGLIPSLLQVLPS 407

BLAST of Carg20540 vs. TAIR 10
Match: AT1G78180.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 449.1 bits (1154), Expect = 8.8e-126
Identity = 251/417 (60.19%), Postives = 302/417 (72.42%), Query Frame = 0

Query: 16  PSQSQSLSCCS-TNSGFFVTGGLFID-SAVPSSFANSIWSKNSSVEESGSVSVSASFCHQ 75
           PS S S S    +N  FF TGGLF++   V SSF +SI SK                C  
Sbjct: 11  PSSSSSTSSIDLSNEAFFSTGGLFLEPPGVSSSFFDSISSK----------------CSD 70

Query: 76  EQPVFFGVLRWRRKPRI---ADCGFLSLSVRGDRFVPEQKVRADGYENCEKAGKCEGALA 135
            +P+ F    WR K R+    +  FLS+S+  DR   EQ+ +    +N E  GK      
Sbjct: 71  SEPLHFPGY-WRNKTRLRSGKNFMFLSVSLSKDR--SEQQCKKALAQNDEIPGK------ 130

Query: 136 TVENDKQEKKYTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFY 195
             +N K+     V +R  MNT KHLW+GA+AAMVS+TF+APLERLKLEY VRGEQ NL  
Sbjct: 131 --DNRKRSVIGGVRRRGTMNTRKHLWAGAVAAMVSKTFLAPLERLKLEYTVRGEQRNLLV 190

Query: 196 VIKKIAASQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNFERFVA 255
           V K IA +QGL GFWKGN +N+LRTAPFKA+NF AYDTYRKQLLK++G +  +NFERFVA
Sbjct: 191 VAKSIATTQGLTGFWKGNLLNVLRTAPFKAVNFCAYDTYRKQLLKIAGNQEATNFERFVA 250

Query: 256 GAAAGITATVLCLPLDTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILSM 315
           GAAAGITATVLCLPLDTIRTK+VA GGEALGG+ GAFRYM+QTEG FSLYKGL+PSI SM
Sbjct: 251 GAAAGITATVLCLPLDTIRTKLVARGGEALGGIGGAFRYMIQTEGLFSLYKGLVPSIASM 310

Query: 316 APSGAVFYTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISGA 375
           A SGAVFY VYDILK+++L +PEGRKR+ +M QQG++LNALD+LELGPIRTL+YGAI+GA
Sbjct: 311 ALSGAVFYGVYDILKSSFLHTPEGRKRLIDMKQQGQELNALDRLELGPIRTLMYGAIAGA 370

Query: 376 CAEAATYPFEVIRRQLQMQVQATRMSAFATCSKIVQQGGIQALYAGLLPSLLQACDS 428
           C E ATYPFEV+RRQLQMQ+   +++A A    I+++GGI ALYAGLLPSLLQ   S
Sbjct: 371 CTEVATYPFEVVRRQLQMQMGKNKLNALAMGFNIIERGGIPALYAGLLPSLLQVLPS 400

BLAST of Carg20540 vs. TAIR 10
Match: AT1G34630.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein (TAIR:AT5G51150.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 448.4 bits (1152), Expect = 1.5e-125
Identity = 234/387 (60.47%), Postives = 285/387 (73.64%), Query Frame = 0

Query: 478 SGGSYCEHCGCGCGSGSADYSSLPAFS---CSSYSLWLDSARLRESGKFWRILVASAKGF 537
           + GS C  C      G+ D+     F+   C +      S  + +S K  RI+VAS KGF
Sbjct: 8   TNGSRCSSCISSDNDGN-DFGKSDEFTCKQCKNSDSKSKSKSVNDSDKLRRIIVASVKGF 67

Query: 538 TIGAGLKGGLSLFSILAGLKRRKALASLGKK-GVITNRDAISMALKETLRYGLFLGTFAG 597
           TIG GLKGGL++FSI+A   RR+  +   +K G  +N +AI+M +KETLRYGLFLGTFAG
Sbjct: 68  TIGTGLKGGLAIFSIVARFARRRRSSPQSRKTGEFSNSEAIAMGIKETLRYGLFLGTFAG 127

Query: 598 TFVSIDEIIGNLAGHRRTARWRALSAGALAGPSMLLTGLNTQHKTLAIYIFMRAAVLAAR 657
           TFVS+DE I  LAG +RTA+WRAL AG +AGPSMLLTG NTQH +LA+YI MRAAVLA+R
Sbjct: 128 TFVSVDEAIAALAGDKRTAKWRALFAGLVAGPSMLLTGPNTQHTSLAVYILMRAAVLASR 187

Query: 658 CGIKSKRFGHICKPLTWSYGDIFLMCLSSSQILQLIRRIIFRSAYVLKQDSLPPSFSSFL 717
           CGIKSKRFG ICKPLTW +GD+FLMCLSSSQIL         SAY+LKQ+SLP S+ SFL
Sbjct: 188 CGIKSKRFGTICKPLTWKHGDLFLMCLSSSQIL---------SAYILKQESLPSSYKSFL 247

Query: 718 NIHGGKDTVILEGLKSFVSGMPSSNKFKAIEKYYRAMGADVKLDLQMKTPCTIIHGNQSC 777
           N  GGKD  IL+G+K   +  P +N  +AIEKYY+++G D+KLD  MK PCTIIHGN+SC
Sbjct: 248 NKQGGKDLSILQGVKDIATAQPFTN-LRAIEKYYKSVGVDIKLDPTMKVPCTIIHGNESC 307

Query: 778 GGHVLNFLIQGYKRALPVYLPVYLVPALIVHREGLMNRPYEILARGLLGTARSSLFLSVY 837
             H + F +Q YKRALPVY+PVYL+PALIVHR+ L+ + Y IL +GLLGTARSSLFL+ Y
Sbjct: 308 VKHGVTFFLQAYKRALPVYVPVYLIPALIVHRQDLLKKQYSILGKGLLGTARSSLFLATY 367

Query: 838 CSSAWMWTCLTARTFKKINIPLVALAT 861
           CSSAW WTCL  RTF+  NIPLVA+AT
Sbjct: 368 CSSAWAWTCLLFRTFETCNIPLVAIAT 383

BLAST of Carg20540 vs. TAIR 10
Match: AT4G32400.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 140.2 bits (352), Expect = 8.7e-33
Identity = 95/297 (31.99%), Postives = 156/297 (52.53%), Query Frame = 0

Query: 133 ENDKQEKK--YTVHKRNAMNTTKHLWSGAIAAMVSRTFVAPLERLKLEYIVRGEQGNLFY 192
           E  K++KK   T+  + A  + + L SGA+A  VSRT VAPLE ++   +V     +   
Sbjct: 90  EKRKRKKKGGLTLKIKIANPSLRRLLSGAVAGAVSRTVVAPLETIRTHLMVGSGGNSSTE 149

Query: 193 VIKKIAASQGLKGFWKGNFVNILRTAPFKALNFYAYDTYRKQLLKLSGRETTSNF-ERFV 252
           V   I   +G  G ++GN VN++R AP +A+  + ++T  K+L    G+E+        +
Sbjct: 150 VFSDIMKHEGWTGLFRGNLVNVIRVAPARAVELFVFETVNKKLSPPHGQESKIPIPASLL 209

Query: 253 AGAAAGITATVLCLPLDTIRTKIVAPGGEALGGVIGAFRYMVQTEGFFSLYKGLLPSILS 312
           AGA AG++ T+L  PL+ ++T++    G    G+  AF  +++ EG   LY+GL PS++ 
Sbjct: 210 AGACAGVSQTLLTYPLELVKTRLTIQRG-VYKGIFDAFLKIIREEGPTELYRGLAPSLIG 269

Query: 313 MAPSGAVFYTVYDILKTAYLQSPEGRKRIQNMNQQGKQLNALDQLELGPIRTLLYGAISG 372
           + P  A  Y  YD L+ AY          ++ ++Q K         +G I TLL G+++G
Sbjct: 270 VVPYAATNYFAYDSLRKAY----------RSFSKQEK---------IGNIETLLIGSLAG 329

Query: 373 ACAEAATYPFEVIRRQLQMQVQATRM---SAFATCSKIVQQGGIQALYAGLLPSLLQ 424
           A +  AT+P EV R+ +Q+   + R+   +       I++  GI   Y GL PS L+
Sbjct: 330 ALSSTATFPLEVARKHMQVGAVSGRVVYKNMLHALVTILEHEGILGWYKGLGPSCLK 366