Carg20515 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20515
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionpolygalacturonase 1 beta-like protein 3
LocationCarg_Chr12: 30853 .. 33309 (-)
RNA-Seq ExpressionCarg20515
SyntenyCarg20515
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAACAGAAATGCACAGATTACCCGCGCCCCTTCTGGTTCCTCTCTATCTCTCTATCTTCTTGCTTTCGGCTTTCAATGTAAGTCAATTTCCATCTCCACTTCACTCTTTATTTCTCTTTACTTCAGTTTTTATCCCTGTGTGGAATTGTTCTGTAAATTGTAATTTGTGCGTGGAATTGTTTTGTAAATTGTAAATTGTAATTTGTGTGTGGTTTATGTGGGTGTGCGGAAACAGGTTCCCGCGGCTGGCGACGACGACAACCCCTTTACCCCAAAAGCTTTTCTGAGTCGTTATTGGAACAAGGAGGTCTGGAATGATTTGCCAAAACCCACTTTTCTCTTCTCCAAGGCGTCTCCCTTGACGGCAGTGCAGTCCGCGGCCTTCGCCAAACTCGCAGCCCAAAACACACTCTCCTCCCGCCTCCCTCAGTTCTGCTCCGCCGCAAATTTGCTCTGCTTCCCCGATTTAGCCCCTTCTTTGGCCAAGCACAACAAGGACTCCAACTTTGCTGTCTATTCCAATAGGAACTTCACCACTTACGGCTCCAAAAGAGCTGGTGGCATCGACTCCTTCAAGAACTATTCCAATGATGAAAATATCTTCGTCGATTCCTTCCGGCGCTACAGCCACGGCGCCTTGAGCCACGCCGACCGATTTTCGACCTATGGCATCGACACCAACGTCCCCGACCAGGGTTTTAACACTTACGCCTCCAAAGCCATAGGAGGTAACGGCAAGTTCACAACCTACGAACAATTCACCAATGTCCCCAATCTCCGATTTACCAGCTACTCCACCGATGCCACTGGACGATCTCAAGTCTTTAAGAGCTACAGCGAGAACGCAAACGCAGGAGATCAGTCGTTTGCCAACTATGGAAAACGAGGAAATGGGGGTCCCAACGAGTTCTCTGCCTACGGGACCAACGCAAACGTAATAGGCTCTGGGTTTTCAAGCTATGGGTCCGACGGAAATGGCGCTAACGACACATTCACCAATTACGGTAACGAGCAAAACGACCCACAGAACCACTTCAGGAGCTATGGGGAGGGTGGGAACGCAGCGATTGAAAGCTTCTCTAGTTACAGAGACCAGGCGAATGTGGGGGACGATTCATTTCAGTCCTACTCCAAGAACTCGAACTCGGCGAAGGTCAATTTCGCAAATTATGGGAAATCCGCCAATGTGGGTTCGGACAAGTTTAGTGGTTATGGACAGAATGGGAATGCGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACAACGTTCAAAGATTACGCGAAACGGGGGATTAGTTTTGCCCAGTACAGCAATGTGAGCTCCGGTGGTGCGAAAATGTCGGTGAACGGGAGTTTGGCGCAGAGGTGGGTAGAGCCCGGGAAGTTCTTCCGCGAGTCAATGCTGAGGAAGGGGACGGTGATGGCTATGCCGGACATCAAGGATAAAATGCCGAAGAGGTCGTTCCTGCCCCGATCTATTCTATCCAAGTTACCCTTTTCGAGTTCGAAACTGGGAGTGATGAAGAAGATATTCCACGCCGAGGATAACTCGTCGATGGAGACTATGATGACGGAGACGCTCAGGGAGTGCGAGAGGGCGCCGAGTGCCGGGGAGACAAAGCGGTGCGTGGGGTCGGTGGAGGATATGATAGACTTCGCCACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGGTCGAAGGGGAACATAATGATAGGGGCGGTGAAAGCGGTGGAGGGAGGGAAGGTGACGAAGTCGGTGTCTTGCCACCAGAGCCTATTCCCTTACCTACTCTACTACTGCCACTCCGTGCCCAAGGTTCGAGTGTACGAAGCGGAATTGCTGGATCCAAGGTCTAAGGCCAACATAAACCACGGAGTTGCCATCTGTCACTTGGACACCTCAGCCTGGAGTCCCAGTCACGGGGCGTTCATAGCTCTCGGGTCCGCCCCCGGTCGGGTGGAGGTGTGCCACTGGATCTTCGAGAACGACATGACGTGGACTGTGGCCGACTGATCTGTCGCCGCAAGGGGGGAGGGTATGGGTGTCGTCTAAAAAATGTTTAGCCGCCACCTCCACGTCATCAGAACATGTTTTTAATGTTTAATTGCTTTTGAATATGATTGGAAATAGAGAGGGGGGAAAAAAAAAAAGAAAAAAAAAAGAGGATATTATTTTCTGTTTCTAAGTTGTTTTTGGTTTCGCCAAAGAATGGTACTGTTAACTGTGAAAGAATGGTCCTGTGCCTATATCTATGTGCTTCCATTGCTTTGTTGTGTTGGGAGGGAAATTCCATGAGACAGCTCACACGCTTGTTGTGACTCTCTCTCTCTCTCTCTCTCTCGCTAAATCTATCCATTCCCCACTTTATGGGCTGCTACTTTGAGTCCAAAACAAGAGAAATTCTTGGTTGAGGTCACAATACATCTAAC

mRNA sequence

CCAAAACAGAAATGCACAGATTACCCGCGCCCCTTCTGGTTCCTCTCTATCTCTCTATCTTCTTGCTTTCGGCTTTCAATGTTCCCGCGGCTGGCGACGACGACAACCCCTTTACCCCAAAAGCTTTTCTGAGTCGTTATTGGAACAAGGAGGTCTGGAATGATTTGCCAAAACCCACTTTTCTCTTCTCCAAGGCGTCTCCCTTGACGGCAGTGCAGTCCGCGGCCTTCGCCAAACTCGCAGCCCAAAACACACTCTCCTCCCGCCTCCCTCAGTTCTGCTCCGCCGCAAATTTGCTCTGCTTCCCCGATTTAGCCCCTTCTTTGGCCAAGCACAACAAGGACTCCAACTTTGCTGTCTATTCCAATAGGAACTTCACCACTTACGGCTCCAAAAGAGCTGGTGGCATCGACTCCTTCAAGAACTATTCCAATGATGAAAATATCTTCGTCGATTCCTTCCGGCGCTACAGCCACGGCGCCTTGAGCCACGCCGACCGATTTTCGACCTATGGCATCGACACCAACGTCCCCGACCAGGGTTTTAACACTTACGCCTCCAAAGCCATAGGAGGTAACGGCAAGTTCACAACCTACGAACAATTCACCAATGTCCCCAATCTCCGATTTACCAGCTACTCCACCGATGCCACTGGACGATCTCAAGTCTTTAAGAGCTACAGCGAGAACGCAAACGCAGGAGATCAGTCGTTTGCCAACTATGGAAAACGAGGAAATGGGGGTCCCAACGAGTTCTCTGCCTACGGGACCAACGCAAACGTAATAGGCTCTGGGTTTTCAAGCTATGGGTCCGACGGAAATGGCGCTAACGACACATTCACCAATTACGGTAACGAGCAAAACGACCCACAGAACCACTTCAGGAGCTATGGGGAGGGTGGGAACGCAGCGATTGAAAGCTTCTCTAGTTACAGAGACCAGGCGAATGTGGGGGACGATTCATTTCAGTCCTACTCCAAGAACTCGAACTCGGCGAAGGTCAATTTCGCAAATTATGGGAAATCCGCCAATGTGGGTTCGGACAAGTTTAGTGGTTATGGACAGAATGGGAATGCGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACAACGTTCAAAGATTACGCGAAACGGGGGATTAGTTTTGCCCAGTACAGCAATGTGAGCTCCGGTGGTGCGAAAATGTCGGTGAACGGGAGTTTGGCGCAGAGGTGGGTAGAGCCCGGGAAGTTCTTCCGCGAGTCAATGCTGAGGAAGGGGACGGTGATGGCTATGCCGGACATCAAGGATAAAATGCCGAAGAGGTCGTTCCTGCCCCGATCTATTCTATCCAAGTTACCCTTTTCGAGTTCGAAACTGGGAGTGATGAAGAAGATATTCCACGCCGAGGATAACTCGTCGATGGAGACTATGATGACGGAGACGCTCAGGGAGTGCGAGAGGGCGCCGAGTGCCGGGGAGACAAAGCGGTGCGTGGGGTCGGTGGAGGATATGATAGACTTCGCCACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGGTCGAAGGGGAACATAATGATAGGGGCGGTGAAAGCGGTGGAGGGAGGGAAGGTGACGAAGTCGGTGTCTTGCCACCAGAGCCTATTCCCTTACCTACTCTACTACTGCCACTCCGTGCCCAAGGTTCGAGTGTACGAAGCGGAATTGCTGGATCCAAGGTCTAAGGCCAACATAAACCACGGAGTTGCCATCTGTCACTTGGACACCTCAGCCTGGAGTCCCAGTCACGGGGCGTTCATAGCTCTCGGGTCCGCCCCCGGTCGGGTGGAGGTGTGCCACTGGATCTTCGAGAACGACATGACGTGGACTGTGGCCGACTGATCTGTCGCCGCAAGGGGGGAGGGTATGGGTGTCGTCTAAAAAATGTTTAGCCGCCACCTCCACGTCATCAGAACATGTTTTTAATGTTTAATTGCTTTTGAATATGATTGGAAATAGAGAGGGGGGAAAAAAAAAAAGAAAAAAAAAAGAGGATATTATTTTCTGTTTCTAAGTTGTTTTTGGTTTCGCCAAAGAATGGTACTGTTAACTGTGAAAGAATGGTCCTGTGCCTATATCTATGTGCTTCCATTGCTTTGTTGTGTTGGGAGGGAAATTCCATGAGACAGCTCACACGCTTGTTGTGACTCTCTCTCTCTCTCTCTCTCTCGCTAAATCTATCCATTCCCCACTTTATGGGCTGCTACTTTGAGTCCAAAACAAGAGAAATTCTTGGTTGAGGTCACAATACATCTAAC

Coding sequence (CDS)

ATGCACAGATTACCCGCGCCCCTTCTGGTTCCTCTCTATCTCTCTATCTTCTTGCTTTCGGCTTTCAATGTTCCCGCGGCTGGCGACGACGACAACCCCTTTACCCCAAAAGCTTTTCTGAGTCGTTATTGGAACAAGGAGGTCTGGAATGATTTGCCAAAACCCACTTTTCTCTTCTCCAAGGCGTCTCCCTTGACGGCAGTGCAGTCCGCGGCCTTCGCCAAACTCGCAGCCCAAAACACACTCTCCTCCCGCCTCCCTCAGTTCTGCTCCGCCGCAAATTTGCTCTGCTTCCCCGATTTAGCCCCTTCTTTGGCCAAGCACAACAAGGACTCCAACTTTGCTGTCTATTCCAATAGGAACTTCACCACTTACGGCTCCAAAAGAGCTGGTGGCATCGACTCCTTCAAGAACTATTCCAATGATGAAAATATCTTCGTCGATTCCTTCCGGCGCTACAGCCACGGCGCCTTGAGCCACGCCGACCGATTTTCGACCTATGGCATCGACACCAACGTCCCCGACCAGGGTTTTAACACTTACGCCTCCAAAGCCATAGGAGGTAACGGCAAGTTCACAACCTACGAACAATTCACCAATGTCCCCAATCTCCGATTTACCAGCTACTCCACCGATGCCACTGGACGATCTCAAGTCTTTAAGAGCTACAGCGAGAACGCAAACGCAGGAGATCAGTCGTTTGCCAACTATGGAAAACGAGGAAATGGGGGTCCCAACGAGTTCTCTGCCTACGGGACCAACGCAAACGTAATAGGCTCTGGGTTTTCAAGCTATGGGTCCGACGGAAATGGCGCTAACGACACATTCACCAATTACGGTAACGAGCAAAACGACCCACAGAACCACTTCAGGAGCTATGGGGAGGGTGGGAACGCAGCGATTGAAAGCTTCTCTAGTTACAGAGACCAGGCGAATGTGGGGGACGATTCATTTCAGTCCTACTCCAAGAACTCGAACTCGGCGAAGGTCAATTTCGCAAATTATGGGAAATCCGCCAATGTGGGTTCGGACAAGTTTAGTGGTTATGGACAGAATGGGAATGCGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACAACGTTCAAAGATTACGCGAAACGGGGGATTAGTTTTGCCCAGTACAGCAATGTGAGCTCCGGTGGTGCGAAAATGTCGGTGAACGGGAGTTTGGCGCAGAGGTGGGTAGAGCCCGGGAAGTTCTTCCGCGAGTCAATGCTGAGGAAGGGGACGGTGATGGCTATGCCGGACATCAAGGATAAAATGCCGAAGAGGTCGTTCCTGCCCCGATCTATTCTATCCAAGTTACCCTTTTCGAGTTCGAAACTGGGAGTGATGAAGAAGATATTCCACGCCGAGGATAACTCGTCGATGGAGACTATGATGACGGAGACGCTCAGGGAGTGCGAGAGGGCGCCGAGTGCCGGGGAGACAAAGCGGTGCGTGGGGTCGGTGGAGGATATGATAGACTTCGCCACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGGTCGAAGGGGAACATAATGATAGGGGCGGTGAAAGCGGTGGAGGGAGGGAAGGTGACGAAGTCGGTGTCTTGCCACCAGAGCCTATTCCCTTACCTACTCTACTACTGCCACTCCGTGCCCAAGGTTCGAGTGTACGAAGCGGAATTGCTGGATCCAAGGTCTAAGGCCAACATAAACCACGGAGTTGCCATCTGTCACTTGGACACCTCAGCCTGGAGTCCCAGTCACGGGGCGTTCATAGCTCTCGGGTCCGCCCCCGGTCGGGTGGAGGTGTGCCACTGGATCTTCGAGAACGACATGACGTGGACTGTGGCCGACTGA

Protein sequence

MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFSKASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKRGNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFKIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVMAMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALGSAPGRVEVCHWIFENDMTWTVAD
Homology
BLAST of Carg20515 vs. NCBI nr
Match: KAG7020075.1 (Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1238.0 bits (3202), Expect = 0.0e+00
Identity = 623/623 (100.00%), Postives = 623/623 (100.00%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS
Sbjct: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR
Sbjct: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT
Sbjct: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR
Sbjct: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA
Sbjct: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM
Sbjct: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP
Sbjct: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Carg20515 vs. NCBI nr
Match: KAG6585155.1 (Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 620/623 (99.52%), Postives = 622/623 (99.84%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MHRLPAPLLVPLYLSIFLLSAFNVPAAGD+DNPFTPKAFLSRYWNKEVWNDLPKPTFLFS
Sbjct: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR
Sbjct: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT
Sbjct: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGGNGKFTTYEQ TNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR
Sbjct: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA
Sbjct: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           KIYGVNTTFKDYAKRGISFAQYSNV+SGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM
Sbjct: 361 KIYGVNTTFKDYAKRGISFAQYSNVNSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP
Sbjct: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Carg20515 vs. NCBI nr
Match: XP_022951186.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 617/623 (99.04%), Postives = 619/623 (99.36%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MHRLPAPLLVPLYLSIFLLSAFNVPAAGD+DNPFTPKAFLSRYWNKEVWNDLPKPTFLFS
Sbjct: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR
Sbjct: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT
Sbjct: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGGNGKFTTYEQ TNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR
Sbjct: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQN+FRSYGEGGN A
Sbjct: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM
Sbjct: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMM ETL ECERAP
Sbjct: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMMETLGECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Carg20515 vs. NCBI nr
Match: XP_023001947.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita maxima])

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 613/623 (98.39%), Postives = 617/623 (99.04%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MHRLPAPLLVPLYLSIFLLSAFNVPAAGD+DNPFTP+AFLSRYWNKEV NDLPKPTFLFS
Sbjct: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR
Sbjct: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYGSKRAGGIDSFKNYSNDENI VDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT
Sbjct: 121 NFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGGNGKFTTYEQ TNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR
Sbjct: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQN+FRSYGEGGN A
Sbjct: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM
Sbjct: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP
Sbjct: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDM+DFATSVLGRNVVVR TENTKGSKGNIMIGAVKAVEGGKVTKSVS
Sbjct: 481 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDPRS ANINHGVAICHLDTSAWSPSHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Carg20515 vs. NCBI nr
Match: XP_023538472.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 612/623 (98.23%), Postives = 617/623 (99.04%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MHRLP PLLVPLYLSIFLLSAFNVPAAGD+DNPFTPKAFLSRYWNKEV NDLPKPTFLFS
Sbjct: 1   MHRLPEPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPKAFLSRYWNKEVRNDLPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR
Sbjct: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYGSKRAGGIDSFKNYSNDENI VDSFRRYSHGALSHADRFSTYGIDTNVPDQGFN+
Sbjct: 121 NFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNS 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGGNGKFTTYEQ TNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSF+NYGKR
Sbjct: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFSNYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQN+FRSYGEGGN A
Sbjct: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSAN GSDKFSGYGQNGNAQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANEGSDKFSGYGQNGNAQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM
Sbjct: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDIKDKMPKRSFLPRSILSKLPFSS+KLGVMKKIFHAEDNSSMETMMTETLRECERAP
Sbjct: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSTKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Carg20515 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 785.0 bits (2026), Expect = 6.1e-226
Identity = 387/622 (62.22%), Postives = 475/622 (76.37%), Query Frame = 0

Query: 12  LYLSIFLLSAFNVPAAGD---------DDNPFTPKAFLSRYWNKEVWNDLPKPTFLFSKA 71
           L L  F    FNV   G          ++NPFTPKA L RYWNKE+    P+  FL SKA
Sbjct: 6   LLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISKA 65

Query: 72  SPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNF 131
           SPL AV SA F+KLAA N+L +R P FCSAANL CFPDL  SL KH+ D  F+VY  +NF
Sbjct: 66  SPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLEKHDDDVKFSVYDQKNF 125

Query: 132 TTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYA 191
           T YG+ RAGG DSFKNYS D N+  DSFRRYS  A  H D+F+ YG ++NV ++GFN+Y 
Sbjct: 126 TNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYG 185

Query: 192 SKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGD-QSFANYGKRG 251
           +   GG G FT Y+   N P  RFT+YS    GRSQ FK+Y+  ANAG+ QSF +YGK G
Sbjct: 186 TFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNG 245

Query: 252 NGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAAI 311
           NG PNEF++YG ++NVIGSGFS+YG  GN ANDTFT+YG++ N PQN+F +YG  GNAA+
Sbjct: 246 NGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAV 305

Query: 312 ESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFK 371
           ++F++YRD+ANVGDDSF SY+K+SNS KVNF NYG+S N GS+ F+GYG+      + FK
Sbjct: 306 DTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFK 365

Query: 372 IYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVMA 431
            Y  N+TFKDYAK+G++FA+Y NVS+  A    +G    +W+EPGKFFRES L++GTV+ 
Sbjct: 366 TYTPNSTFKDYAKKGVAFAKY-NVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIP 425

Query: 432 MPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPS 491
           MPDIKDKMPKRSFLPRSI++KLPFS+SKLG +K+IFHA +NS+M  ++T+ + ECER PS
Sbjct: 426 MPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERPPS 485

Query: 492 AGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVSC 551
            GETKRCVGS EDMIDFATSVLGR+VV+RTTEN  GSK  ++IG V  + GGK+TK+VSC
Sbjct: 486 VGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSC 545

Query: 552 HQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALGS 611
           HQSL+PYLLYYCHSVPKVRVYEA+LL+  SK  INHG+AICH+DTS+W PSHGAF+ALGS
Sbjct: 546 HQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLALGS 605

Query: 612 APGRVEVCHWIFENDMTWTVAD 624
            PGR+EVCHWIFENDM W +AD
Sbjct: 606 KPGRIEVCHWIFENDMNWAIAD 626

BLAST of Carg20515 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 766.9 bits (1979), Expect = 1.7e-220
Identity = 378/623 (60.67%), Postives = 465/623 (74.64%), Query Frame = 0

Query: 12  LYLSIFLLSAFNVPAAGDDD----------NPFTPKAFLSRYWNKEVWNDLPKPTFLFSK 71
           ++L  FL   ++V  AG +           NPFTPKA L RYWNK++ +  P   FL SK
Sbjct: 6   VFLLPFLSRLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLLSK 65

Query: 72  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRN 131
           ASPLTAV SA FAKLA++N L  RLP FCSAANL CFPDL P+L KH+ D  F+VY  RN
Sbjct: 66  ASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQRN 125

Query: 132 FTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTY 191
           FT YG+   GG DSFK YS + N   DSFRRYS  A  H D+F+ Y  ++NV ++ FN+Y
Sbjct: 126 FTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSY 185

Query: 192 ASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAG-DQSFANYGKR 251
            + A GG+G F  Y+   N P  RF +YS +  GR+Q FK+Y+  AN+G  QSF +YGK 
Sbjct: 186 GTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKN 245

Query: 252 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 311
           GNG PNEFS+YG ++NVIGS FS+YG +GN ANDTF  YG + N PQN+F+SYG  GNAA
Sbjct: 246 GNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAA 305

Query: 312 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 371
           +++F +YRD ANVGDDSF SY+KNSN  KV+F NYGKS N GS+ FSGYG+      + F
Sbjct: 306 VDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEGNKIDF 365

Query: 372 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 431
           K Y  N+TFKDY K G+ FA+Y+  S GG      G    +WVEPGKFFRESML++GT++
Sbjct: 366 KTYTQNSTFKDYTKTGVEFAKYNRSSLGG------GKTVNKWVEPGKFFRESMLKEGTLI 425

Query: 432 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 491
            MPDIKDKMPKRSFLPRSI+SKLPFS+SK+  +K++FHA DNS+ME ++T+ +RECER P
Sbjct: 426 WMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPP 485

Query: 492 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 551
           +  ETKRCVGS EDMIDFATSVLGR+VV+RTTE+  GSK  +MIG V  + GG+VTKSVS
Sbjct: 486 TVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVS 545

Query: 552 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 611
           CHQSL+PYLLYYCHSVPKVRVYE++LLDP+SKA INHG+AICH+DTSAW  +HGAF+ LG
Sbjct: 546 CHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLG 605

Query: 612 SAPGRVEVCHWIFENDMTWTVAD 624
           S PG++EVCHWIFENDM W +AD
Sbjct: 606 SRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Carg20515 vs. ExPASy Swiss-Prot
Match: Q40161 (Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 GN=GP1 PE=1 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 9.5e-219
Identity = 385/620 (62.10%), Postives = 464/620 (74.84%), Query Frame = 0

Query: 12  LYLSIFLLSAFNVPAAGDDD--NPFTPKAFLSRYWNKEVWNDLPKPTFLFSKASPLTAVQ 71
           L L +F L +FNV   GD +  NPFTPK +L RYW K++ NDLPKP FL +KASPL A Q
Sbjct: 12  LLLLLFSLPSFNVVVGGDGESGNPFTPKGYLIRYWKKQISNDLPKPWFLLNKASPLNAAQ 71

Query: 72  SAAFAKLAA-QNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNFTTYGSK 131
            A + KL A QN L+++L  FCS+ANL+C PDL+PSL KH+ D +FA YS++NFT YG+ 
Sbjct: 72  YATYTKLVADQNALTTQLHTFCSSANLMCAPDLSPSLEKHSGDIHFATYSDKNFTNYGTN 131

Query: 132 RAG-GIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYASKAIG 191
             G G+++FKNYS  ENI V+SFRRY  G+    ++F  Y  D NV DQ FN+Y++   G
Sbjct: 132 EPGIGVNTFKNYSEGENIPVNSFRRYGRGS-PRDNKFDNYASDGNVIDQSFNSYSTSTAG 191

Query: 192 GNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKRGNGGPNE 251
           G+GKFT Y    N PNL FTSYS   TG  Q F  YS+ ANAGDQ F +YGK GNG   E
Sbjct: 192 GSGKFTNYAANANDPNLHFTSYSDQGTGGVQKFTIYSQEANAGDQYFKSYGKNGNGANGE 251

Query: 252 FSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAAIESFSSY 311
           F +YG + NVIGS F++YG   NG +  FT+YG   N P+NHF +YG GGN   E+F+SY
Sbjct: 252 FVSYGNDTNVIGSTFTNYGQTANGGDQKFTSYGFNGNVPENHFTNYGAGGNGPSETFNSY 311

Query: 312 RDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFKIYGVNT 371
           RDQ+NVGDD+F +Y K++N  + NF NYG+S N G+D F+ YG+ GN   + FK YGVN 
Sbjct: 312 RDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGGNDPHINFKTYGVNN 371

Query: 372 TFKDYAKRGISFAQYSNVSSG--GAKMSVNG--SLAQRWVEPGKFFRESMLRKGTVMAMP 431
           TFKDY K   +F+ Y N +S    + M VNG   +  RWVEPGKFFRE ML+ GT+M MP
Sbjct: 372 TFKDYVKDTATFSNYHNKTSQVLASLMEVNGGKKVNNRWVEPGKFFREKMLKSGTIMPMP 431

Query: 432 DIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSAG 491
           DIKDKMPKRSFLPR I SKLPFS+SK+  +KKIFHA D S +E M+ + L ECERAPSAG
Sbjct: 432 DIKDKMPKRSFLPRVIASKLPFSTSKIAELKKIFHAGDESQVEKMIGDALSECERAPSAG 491

Query: 492 ETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVSCHQ 551
           ETKRCV S EDMIDFATSVLGRNVVVRTTE+TKGS GNIMIG+VK + GGKVTKSVSCHQ
Sbjct: 492 ETKRCVNSAEDMIDFATSVLGRNVVVRTTEDTKGSNGNIMIGSVKGINGGKVTKSVSCHQ 551

Query: 552 SLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALGSAP 611
           +L+PYLLYYCHSVPKVRVYEA++LDP SK  INHGVAICH+DTS+W PSHGAF+ALGS P
Sbjct: 552 TLYPYLLYYCHSVPKVRVYEADILDPNSKVKINHGVAICHVDTSSWGPSHGAFVALGSGP 611

Query: 612 GRVEVCHWIFENDMTWTVAD 624
           G++EVCHWIFENDMTW +AD
Sbjct: 612 GKIEVCHWIFENDMTWAIAD 630

BLAST of Carg20515 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 4.6e-213
Identity = 375/623 (60.19%), Postives = 449/623 (72.07%), Query Frame = 0

Query: 12  LYLSIF-LLSAFNVPAAG---------DDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFSK 71
           L+L  F + S+ NV  AG           +NPFTPKA L RYWN  +  D PKP+F  SK
Sbjct: 8   LFLCFFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSK 67

Query: 72  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAP-SLAKHNKDSNFAVYSNR 131
           ASPLTAV S  FA LA+ + L++    FCSAA L CFP+LA  SL KH  D NFA YS +
Sbjct: 68  ASPLTAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGK 127

Query: 132 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 191
           NFT YGS R  G DSFKNYS  +NI VDSFRRYS  +  H D F+ Y  + NV DQ F T
Sbjct: 128 NFTNYGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTT 187

Query: 192 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 251
           YA+   GG+G+FT Y    N PN RFTSYS  A GRSQ F +YSEN N G QSF +Y K 
Sbjct: 188 YATGTTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKN 247

Query: 252 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 311
           GNG PNEFS YGT +NV+ +GF+ YG   NGAND+FT+YG   N P N F+ YG+GGN A
Sbjct: 248 GNGAPNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGA 307

Query: 312 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 371
           +  F +YRDQ+N+G DSF SY+KNSN+ KVNF NYGKS N+GSD F+GYGQ+    +V F
Sbjct: 308 VYGFKNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSF 367

Query: 372 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 431
           K YG   +FK Y K G+ FA+YSN       +S NG    +WVE GKFFRE+ML++GT+M
Sbjct: 368 KTYGQGQSFKVYTKDGVVFARYSN------NVSSNGKTVNKWVEEGKFFREAMLKEGTLM 427

Query: 432 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 491
            MPDIKDKMPKR+FLPR+I+  LPFSSS +G + ++F A +NSSM  +++  + ECER  
Sbjct: 428 QMPDIKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPA 487

Query: 492 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 551
           S GETKRCVGS EDMIDFATSVLGR VVVRTTEN  GSK  ++IG V  + GG VT++VS
Sbjct: 488 SHGETKRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVS 547

Query: 552 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 611
           CHQSL+PYLLYYCHSVP+VRVYE +LLDP+S   INHGVAICH+DTSAWSPSHGAF+ALG
Sbjct: 548 CHQSLYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALG 607

Query: 612 SAPGRVEVCHWIFENDMTWTVAD 624
           S PG++EVCHWIFENDMTW + D
Sbjct: 608 SGPGQIEVCHWIFENDMTWNIID 624

BLAST of Carg20515 vs. ExPASy Swiss-Prot
Match: P93217 (Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 GN=GP2 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 1.9e-211
Identity = 371/621 (59.74%), Postives = 458/621 (73.75%), Query Frame = 0

Query: 12  LYLSIFLLSAFNVPAAGDDD---NPFTPKAFLSRYWNKEVWNDLPKPTFLFSKASPLTAV 71
           L + +F LS+FN+  A D D   NPFTPK ++ RYWNK V NDLPKP FL +KASPL A 
Sbjct: 12  LLVLLFSLSSFNIVVAKDGDESGNPFTPKGYVIRYWNKHVSNDLPKPWFLLNKASPLNAA 71

Query: 72  QSAAFAKLAA-QNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNFTTYGS 131
           Q A + KL A QN LS+ L  FCS+ANL+C PDL PSL KH  D +F  Y N+NFT YG+
Sbjct: 72  QYATYTKLVADQNALSTHLQSFCSSANLMCAPDLLPSLEKHTGDIHFTTYGNKNFTNYGT 131

Query: 132 KRAG-GIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYASKAI 191
              G G+++FKNYS D +  V+SFRRY  G+    ++F  Y  D NV DQ FN+Y++   
Sbjct: 132 NEPGIGVNTFKNYSEDAS--VNSFRRYGRGS-PRDNKFDNYAPDGNVIDQSFNSYSTNTP 191

Query: 192 GGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKRGNGGPN 251
           GG+G+FT Y   TNVP+LRFT+YS   TG  Q FK+Y E  N+G QSF +YGK GNG  +
Sbjct: 192 GGSGQFTNYAPNTNVPDLRFTAYSDQGTGGEQEFKTYLEQGNSGGQSFKSYGKNGNGADS 251

Query: 252 EFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAAIESFSS 311
           +F++YG   NV  S F +YG + NG N  FT+Y    N+PQN+F++YG GGN   E+F++
Sbjct: 252 KFTSYGNETNVAASTFKNYGQNANGENQNFTSYSTNGNNPQNNFKNYGVGGNGPSETFTN 311

Query: 312 YRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFKIYGVN 371
           YRD++NVGDD F +Y K++N+ + NF NYG+S N G+D F  YG+ GN   + FK YGVN
Sbjct: 312 YRDESNVGDDKFSNYVKDANAGEANFTNYGQSFNEGTDVFITYGKGGNDPHINFKTYGVN 371

Query: 372 TTFKDYAKRGISFAQYSNVSSG--GAKMSVNG--SLAQRWVEPGKFFRESMLRKGTVMAM 431
            TFKDY K   +F+ Y N +S    +   VNG   +  RW+EPGKFFRE ML+ GT+M M
Sbjct: 372 NTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGKKVNNRWIEPGKFFREKMLKSGTIMPM 431

Query: 432 PDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSA 491
           PDIKDKMPKRSFLPR+I +KLPFS+SK+  +KKIFHA ++S +  M+ + L ECERAPS 
Sbjct: 432 PDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDALSECERAPSP 491

Query: 492 GETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVSCH 551
           GETK+CV S EDMIDFATSVLGRNVVVRTTENT GSKGNIMIG++K + GGKVTKSVSCH
Sbjct: 492 GETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVTKSVSCH 551

Query: 552 QSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALGSA 611
           Q+L+P LLYYCHSVPKVRVYEA++LDP SKA INHGVAICH+DTS+W P HGAFIALGS 
Sbjct: 552 QTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPRHGAFIALGSG 611

Query: 612 PGRVEVCHWIFENDMTWTVAD 624
           PG++EVCHWIFENDMTW  AD
Sbjct: 612 PGKIEVCHWIFENDMTWATAD 629

BLAST of Carg20515 vs. ExPASy TrEMBL
Match: A0A6J1GGV5 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111454108 PE=4 SV=1)

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 617/623 (99.04%), Postives = 619/623 (99.36%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MHRLPAPLLVPLYLSIFLLSAFNVPAAGD+DNPFTPKAFLSRYWNKEVWNDLPKPTFLFS
Sbjct: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR
Sbjct: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT
Sbjct: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGGNGKFTTYEQ TNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR
Sbjct: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQN+FRSYGEGGN A
Sbjct: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM
Sbjct: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMM ETL ECERAP
Sbjct: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMMETLGECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Carg20515 vs. ExPASy TrEMBL
Match: A0A6J1KI28 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111495975 PE=4 SV=1)

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 613/623 (98.39%), Postives = 617/623 (99.04%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MHRLPAPLLVPLYLSIFLLSAFNVPAAGD+DNPFTP+AFLSRYWNKEV NDLPKPTFLFS
Sbjct: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR
Sbjct: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYGSKRAGGIDSFKNYSNDENI VDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT
Sbjct: 121 NFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGGNGKFTTYEQ TNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR
Sbjct: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQN+FRSYGEGGN A
Sbjct: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM
Sbjct: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP
Sbjct: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDM+DFATSVLGRNVVVR TENTKGSKGNIMIGAVKAVEGGKVTKSVS
Sbjct: 481 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDPRS ANINHGVAICHLDTSAWSPSHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Carg20515 vs. ExPASy TrEMBL
Match: A0A6J1HB03 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111462438 PE=4 SV=1)

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 554/623 (88.92%), Postives = 587/623 (94.22%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MH LPAPLLV LYL IFLLS  NVPAA D+DNPFTPKAFL+RYWNKEV NDLPKPTFLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDLPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           K SPLTAVQSAAFAKLAAQN LSSRLP+FCSAANLLCFPDL+PSLAKH+  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYG++R+GGIDSFKNYSN +N+ VDSFRRYSH AL H D+FSTYG DTNVPDQ FNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKA+GGNG FTTYEQ  NVPNLRFT+YS+DATGRSQ FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEF+ YGTNANVIGSGFSSYG++GNGANDTFTNYGNEQN+PQN+FRSYG+GGN A
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKS N G+DKFSGYGQNGN QSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           K YGVNTTFKDYAK+GISFA+YSNVSSGGAKMSV+ SLA+RWVEPGKFFRESMLRKGTVM
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDI+DKMPKRSFLPRSILSKLPFSSSKL VMK+IFHA DNSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVK +EGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDP SKA INHGVAICHLDTSAWSP+HGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Carg20515 vs. ExPASy TrEMBL
Match: A0A6J1K271 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491752 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 552/623 (88.60%), Postives = 587/623 (94.22%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MH LPAPLLV LYL IFLLS  NVPAA D+DNPFTPKAFL+RYWNKEV ND+PKPTFLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDMPKPTFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           K SPLTAVQSAAFAKLAAQN LSSRLP+FCSAANLLCFPDL+PSLAKH+  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYG++R+GGIDSFKNYSN +N+ VDSFRRYSH AL H D+FSTYG DTNVPDQ FNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKA+GGNG FTTYEQ  NVPNLRFT+YS+DATGRSQ FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEF+ YGTNANVIGSGFSSYG++GNGANDTFTNYGNEQN+PQN+FRSYG+GGN A
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKS N G+DKFSGYGQNGN QSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           K YGVNTTFKDYAK+GISFA+YSNVSSGGAKMSV+ SLA+RWVEPGKFFRESMLRKGTV+
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVV 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDI+DKMPKRSFLPRSILSKLPFSSSKL VMK+IFHA DNSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVK +EGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEAELLDP SKA INHGVAICHLDTSAWSP+HGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKAIINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Carg20515 vs. ExPASy TrEMBL
Match: A0A5A7V5U8 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002300 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 545/623 (87.48%), Postives = 587/623 (94.22%), Query Frame = 0

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFS 60
           MHRLP   +VPLYL IF LSA NVPA GD+DNPFTPKAFL+RYWNKEV ND+PKP+FLFS
Sbjct: 1   MHRLPTLHVVPLYLFIFSLSALNVPAVGDEDNPFTPKAFLNRYWNKEVRNDIPKPSFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAA+N LSSRLP FCSAANLLCFPDL+PSLAKH++DSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYG++R+GGIDSFKNYSN +N+ VDSFRRYSH AL H DRFSTYG +TNVPDQ FNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSFNT 180

Query: 181 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGG+GKFTTYEQ  NVPNLRFT+YS+DATGRSQ FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 300
           GNGGPNEF+ YGT+ANV+GS FSSYG+DGNGANDTFTNYGN+QN+PQN+FRSYGEGGN A
Sbjct: 241 GNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKS N G+DKFSGYGQNGN QSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           K+YGVNTTFKDYAK+GI+FA+YSNVSSGGAK SV+GSLA+RWVEPGKFFRESMLRKG+VM
Sbjct: 361 KVYGVNTTFKDYAKQGINFAKYSNVSSGGAKTSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDI+DKMPKRSFLPRSILSKLPFSSSKLGVMK+IFHA DNSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERVP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIG VK + GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE +LLDP SKA INHGVAICHLDTSAWSP+HGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Carg20515 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 785.0 bits (2026), Expect = 4.4e-227
Identity = 387/622 (62.22%), Postives = 475/622 (76.37%), Query Frame = 0

Query: 12  LYLSIFLLSAFNVPAAGD---------DDNPFTPKAFLSRYWNKEVWNDLPKPTFLFSKA 71
           L L  F    FNV   G          ++NPFTPKA L RYWNKE+    P+  FL SKA
Sbjct: 6   LLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISKA 65

Query: 72  SPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNF 131
           SPL AV SA F+KLAA N+L +R P FCSAANL CFPDL  SL KH+ D  F+VY  +NF
Sbjct: 66  SPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLEKHDDDVKFSVYDQKNF 125

Query: 132 TTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYA 191
           T YG+ RAGG DSFKNYS D N+  DSFRRYS  A  H D+F+ YG ++NV ++GFN+Y 
Sbjct: 126 TNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYG 185

Query: 192 SKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGD-QSFANYGKRG 251
           +   GG G FT Y+   N P  RFT+YS    GRSQ FK+Y+  ANAG+ QSF +YGK G
Sbjct: 186 TFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNG 245

Query: 252 NGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAAI 311
           NG PNEF++YG ++NVIGSGFS+YG  GN ANDTFT+YG++ N PQN+F +YG  GNAA+
Sbjct: 246 NGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAV 305

Query: 312 ESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFK 371
           ++F++YRD+ANVGDDSF SY+K+SNS KVNF NYG+S N GS+ F+GYG+      + FK
Sbjct: 306 DTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFK 365

Query: 372 IYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVMA 431
            Y  N+TFKDYAK+G++FA+Y NVS+  A    +G    +W+EPGKFFRES L++GTV+ 
Sbjct: 366 TYTPNSTFKDYAKKGVAFAKY-NVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIP 425

Query: 432 MPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPS 491
           MPDIKDKMPKRSFLPRSI++KLPFS+SKLG +K+IFHA +NS+M  ++T+ + ECER PS
Sbjct: 426 MPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERPPS 485

Query: 492 AGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVSC 551
            GETKRCVGS EDMIDFATSVLGR+VV+RTTEN  GSK  ++IG V  + GGK+TK+VSC
Sbjct: 486 VGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSC 545

Query: 552 HQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALGS 611
           HQSL+PYLLYYCHSVPKVRVYEA+LL+  SK  INHG+AICH+DTS+W PSHGAF+ALGS
Sbjct: 546 HQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLALGS 605

Query: 612 APGRVEVCHWIFENDMTWTVAD 624
            PGR+EVCHWIFENDM W +AD
Sbjct: 606 KPGRIEVCHWIFENDMNWAIAD 626

BLAST of Carg20515 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 785.0 bits (2026), Expect = 4.4e-227
Identity = 387/622 (62.22%), Postives = 475/622 (76.37%), Query Frame = 0

Query: 12  LYLSIFLLSAFNVPAAGD---------DDNPFTPKAFLSRYWNKEVWNDLPKPTFLFSKA 71
           L L  F    FNV   G          ++NPFTPKA L RYWNKE+    P+  FL SKA
Sbjct: 6   LLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISKA 65

Query: 72  SPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNF 131
           SPL AV SA F+KLAA N+L +R P FCSAANL CFPDL  SL KH+ D  F+VY  +NF
Sbjct: 66  SPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLEKHDDDVKFSVYDQKNF 125

Query: 132 TTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYA 191
           T YG+ RAGG DSFKNYS D N+  DSFRRYS  A  H D+F+ YG ++NV ++GFN+Y 
Sbjct: 126 TNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYG 185

Query: 192 SKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGD-QSFANYGKRG 251
           +   GG G FT Y+   N P  RFT+YS    GRSQ FK+Y+  ANAG+ QSF +YGK G
Sbjct: 186 TFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNG 245

Query: 252 NGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAAI 311
           NG PNEF++YG ++NVIGSGFS+YG  GN ANDTFT+YG++ N PQN+F +YG  GNAA+
Sbjct: 246 NGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAV 305

Query: 312 ESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFK 371
           ++F++YRD+ANVGDDSF SY+K+SNS KVNF NYG+S N GS+ F+GYG+      + FK
Sbjct: 306 DTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFK 365

Query: 372 IYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVMA 431
            Y  N+TFKDYAK+G++FA+Y NVS+  A    +G    +W+EPGKFFRES L++GTV+ 
Sbjct: 366 TYTPNSTFKDYAKKGVAFAKY-NVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIP 425

Query: 432 MPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPS 491
           MPDIKDKMPKRSFLPRSI++KLPFS+SKLG +K+IFHA +NS+M  ++T+ + ECER PS
Sbjct: 426 MPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERPPS 485

Query: 492 AGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVSC 551
            GETKRCVGS EDMIDFATSVLGR+VV+RTTEN  GSK  ++IG V  + GGK+TK+VSC
Sbjct: 486 VGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSC 545

Query: 552 HQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALGS 611
           HQSL+PYLLYYCHSVPKVRVYEA+LL+  SK  INHG+AICH+DTS+W PSHGAF+ALGS
Sbjct: 546 HQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLALGS 605

Query: 612 APGRVEVCHWIFENDMTWTVAD 624
            PGR+EVCHWIFENDM W +AD
Sbjct: 606 KPGRIEVCHWIFENDMNWAIAD 626

BLAST of Carg20515 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 766.9 bits (1979), Expect = 1.2e-221
Identity = 378/623 (60.67%), Postives = 465/623 (74.64%), Query Frame = 0

Query: 12  LYLSIFLLSAFNVPAAGDDD----------NPFTPKAFLSRYWNKEVWNDLPKPTFLFSK 71
           ++L  FL   ++V  AG +           NPFTPKA L RYWNK++ +  P   FL SK
Sbjct: 6   VFLLPFLSRLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLLSK 65

Query: 72  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRN 131
           ASPLTAV SA FAKLA++N L  RLP FCSAANL CFPDL P+L KH+ D  F+VY  RN
Sbjct: 66  ASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQRN 125

Query: 132 FTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTY 191
           FT YG+   GG DSFK YS + N   DSFRRYS  A  H D+F+ Y  ++NV ++ FN+Y
Sbjct: 126 FTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSY 185

Query: 192 ASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAG-DQSFANYGKR 251
            + A GG+G F  Y+   N P  RF +YS +  GR+Q FK+Y+  AN+G  QSF +YGK 
Sbjct: 186 GTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKN 245

Query: 252 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 311
           GNG PNEFS+YG ++NVIGS FS+YG +GN ANDTF  YG + N PQN+F+SYG  GNAA
Sbjct: 246 GNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAA 305

Query: 312 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 371
           +++F +YRD ANVGDDSF SY+KNSN  KV+F NYGKS N GS+ FSGYG+      + F
Sbjct: 306 VDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEGNKIDF 365

Query: 372 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 431
           K Y  N+TFKDY K G+ FA+Y+  S GG      G    +WVEPGKFFRESML++GT++
Sbjct: 366 KTYTQNSTFKDYTKTGVEFAKYNRSSLGG------GKTVNKWVEPGKFFRESMLKEGTLI 425

Query: 432 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 491
            MPDIKDKMPKRSFLPRSI+SKLPFS+SK+  +K++FHA DNS+ME ++T+ +RECER P
Sbjct: 426 WMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPP 485

Query: 492 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 551
           +  ETKRCVGS EDMIDFATSVLGR+VV+RTTE+  GSK  +MIG V  + GG+VTKSVS
Sbjct: 486 TVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVS 545

Query: 552 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 611
           CHQSL+PYLLYYCHSVPKVRVYE++LLDP+SKA INHG+AICH+DTSAW  +HGAF+ LG
Sbjct: 546 CHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLG 605

Query: 612 SAPGRVEVCHWIFENDMTWTVAD 624
           S PG++EVCHWIFENDM W +AD
Sbjct: 606 SRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Carg20515 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 742.3 bits (1915), Expect = 3.2e-214
Identity = 375/623 (60.19%), Postives = 449/623 (72.07%), Query Frame = 0

Query: 12  LYLSIF-LLSAFNVPAAG---------DDDNPFTPKAFLSRYWNKEVWNDLPKPTFLFSK 71
           L+L  F + S+ NV  AG           +NPFTPKA L RYWN  +  D PKP+F  SK
Sbjct: 8   LFLCFFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSK 67

Query: 72  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAP-SLAKHNKDSNFAVYSNR 131
           ASPLTAV S  FA LA+ + L++    FCSAA L CFP+LA  SL KH  D NFA YS +
Sbjct: 68  ASPLTAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGK 127

Query: 132 NFTTYGSKRAGGIDSFKNYSNDENIFVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 191
           NFT YGS R  G DSFKNYS  +NI VDSFRRYS  +  H D F+ Y  + NV DQ F T
Sbjct: 128 NFTNYGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTT 187

Query: 192 YASKAIGGNGKFTTYEQFTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 251
           YA+   GG+G+FT Y    N PN RFTSYS  A GRSQ F +YSEN N G QSF +Y K 
Sbjct: 188 YATGTTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKN 247

Query: 252 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNHFRSYGEGGNAA 311
           GNG PNEFS YGT +NV+ +GF+ YG   NGAND+FT+YG   N P N F+ YG+GGN A
Sbjct: 248 GNGAPNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGA 307

Query: 312 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 371
           +  F +YRDQ+N+G DSF SY+KNSN+ KVNF NYGKS N+GSD F+GYGQ+    +V F
Sbjct: 308 VYGFKNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSF 367

Query: 372 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 431
           K YG   +FK Y K G+ FA+YSN       +S NG    +WVE GKFFRE+ML++GT+M
Sbjct: 368 KTYGQGQSFKVYTKDGVVFARYSN------NVSSNGKTVNKWVEEGKFFREAMLKEGTLM 427

Query: 432 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 491
            MPDIKDKMPKR+FLPR+I+  LPFSSS +G + ++F A +NSSM  +++  + ECER  
Sbjct: 428 QMPDIKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPA 487

Query: 492 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKAVEGGKVTKSVS 551
           S GETKRCVGS EDMIDFATSVLGR VVVRTTEN  GSK  ++IG V  + GG VT++VS
Sbjct: 488 SHGETKRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVS 547

Query: 552 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSKANINHGVAICHLDTSAWSPSHGAFIALG 611
           CHQSL+PYLLYYCHSVP+VRVYE +LLDP+S   INHGVAICH+DTSAWSPSHGAF+ALG
Sbjct: 548 CHQSLYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALG 607

Query: 612 SAPGRVEVCHWIFENDMTWTVAD 624
           S PG++EVCHWIFENDMTW + D
Sbjct: 608 SGPGQIEVCHWIFENDMTWNIID 624

BLAST of Carg20515 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 121.3 bits (303), Expect = 2.7e-27
Identity = 75/197 (38.07%), Postives = 100/197 (50.76%), Query Frame = 0

Query: 431 KRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSAGETKRCVG 490
           K +FLPR     +PF S K     K F  E  S    MM +T+ ECE    +GE K C  
Sbjct: 201 KTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEEAEMMKKTIEECEARKVSGEEKYCAT 260

Query: 491 SVEDMIDFATSVLGR-NVVVRTTE----NTKGSKGNIMIGAVKAVEGGKVTKSVSCHQSL 550
           S+E M+DF+ S LG+ +V   +TE    N    K  I    VK +      KSV CH+  
Sbjct: 261 SLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKYKIAAAGVKKLSD---DKSVVCHKQK 320

Query: 551 FPYLLYYCHSVPKVRVYEAELLDP---RSKANINHGVAICHLDTSAWSPSHGAFIALGSA 610
           +P+ ++YCH      VY   L      R+KA     VA+CH +TSAW+P+H AF  L   
Sbjct: 321 YPFAVFYCHKAMMTTVYAVPLEGENGMRAKA-----VAVCHKNTSAWNPNHLAFKVLKVK 380

Query: 611 PGRVEVCHWIFENDMTW 620
           PG V VCH++ E  + W
Sbjct: 381 PGTVPVCHFLPETHVVW 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7020075.10.0e+00100.00Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6585155.10.0e+0099.52Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022951186.10.0e+0099.04polygalacturonase 1 beta-like protein 3 [Cucurbita moschata][more]
XP_023001947.10.0e+0098.39polygalacturonase 1 beta-like protein 3 [Cucurbita maxima][more]
XP_023538472.10.0e+0098.23polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P929906.1e-22662.22Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 ... [more]
P929821.7e-22060.67Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 ... [more]
Q401619.5e-21962.10Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 G... [more]
O807604.6e-21360.19Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 ... [more]
P932171.9e-21159.74Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 G... [more]
Match NameE-valueIdentityDescription
A0A6J1GGV50.0e+0099.04polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KI280.0e+0098.39polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111495... [more]
A0A6J1HB030.0e+0088.92polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K2710.0e+0088.60polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491... [more]
A0A5A7V5U80.0e+0087.48Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
AT1G70370.14.4e-22762.22polygalacturonase 2 [more]
AT1G70370.24.4e-22762.22polygalacturonase 2 [more]
AT1G23760.11.2e-22160.67BURP domain-containing protein [more]
AT1G60390.13.2e-21460.19polygalacturonase 1 [more]
AT5G25610.12.7e-2738.07BURP domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004873BURP domainSMARTSM01045BURP_2coord: 406..622
e-value: 5.0E-133
score: 457.9
IPR004873BURP domainPFAMPF03181BURPcoord: 408..620
e-value: 2.5E-66
score: 223.3
IPR004873BURP domainPROSITEPS51277BURPcoord: 408..622
score: 73.415703
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..291
NoneNo IPR availablePANTHERPTHR31458POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2coord: 13..623
NoneNo IPR availablePANTHERPTHR31458:SF2POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2coord: 13..623

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20515-RACarg20515-RAmRNA