Carg20474 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20474
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr12: 208309 .. 209926 (-)
RNA-Seq Expression	Carg20474
Synteny	Carg20474

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGACGCCGCTGTCCCTTCCTTATCGGTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGGGTGTTCTGTAAACACGTTCTTGAAGAAAAGTATTTGGTGGGTATATTATTTTTGGGGGTTTCGAAGTTGTGGGATTATGTATTATCGGCTATCGAGGTCTTCTTGTAAGGATTCTCTGAAAGTCCTCGAAGAAGATGTAAATCGCGCCAATGCTCTGTGAGAATCCCTTTTCCCTTCACTTTTTTATCTTCCCAGTTCATAGAAATTTCGATCGGTTTAGTGGTTTTGGTGAAATTATGGTGGGAAACTATGGTGGGTTTGCAGGGCTGTTGCAATTCCAAGAGCCAAACATGGTTCATATCTCCAAATGAAATTGGTTTACAGTCAATTAGCTCCACTGTTTCTGTTTTTGATGCAATGGGTGGATTGTTCTTGCACATGTGTTCTTCCCAGATACCTAAATTTCTTCCACATACTTGTTTACAAGGCGCGTCTCTCTTTTCTTCACTTCTTTAATGTGGCTTTGTTTTTTATGTTTATGTGTCTTAGTTTGTTCTGTCAAATGATGTTGTTCAGGTTTACACAGATGGAAGACCCAACCTGTCAGCCCATGGAAGAAAGGCCACTCTCAAAGATTTTTATGGTACATTTTCTTGCCTTCAAACACTCTGTTTTGGAGTTTTGTTTCCCAAACACTTTCACACACATCCATGATTGTAGGAGCATGAACTTAAAGCGAGTAGATATTGTCCTCTTTGAGCATTTTCTTTCCGTCTTCCCCTCAAAGTTTTAAAAACACGACTGATAGGGAGAGGTTTCTACATTCTTATAAGAAATGTTTCGTTCCCCTCTTCAACCGATGTAGGATCTCACATTATGCCCCGTGTTTGATATCATTAGGCTTGGTCTATTGTGGTATTGATATTGTATGAGTTTGAGTATATGAGCTTGTTTTGAGTCCAACAAACGTGCTACTTCAGCTGTGATATTGCCATCCCTTCAACGGCTCCAAGCGAACGCCAGGGAGTTGGATAACGCTAAAGAGAAGCGTGTGGGAGTCGAAGGAGATGGAAAGCTCTCCAATGGTGATGTAGAGAGAGAAGAAGAATGTGGGATTTGCTTAGAAGCTTCCACAAAGATGGTGTTGCCAAACTGCTGCCATTCAATGTGCATCAAATGCTACCACAATTGGTAACTACACATACATACAAACCTTCCTAGATCATCACCATCGGGCTCTGGGCTCGACTGCGTTAATGTCTTTGCTTGTGAATGATTTGTAGGAACACGAGATCGGAGTCGTGCCCGTTTTGTCGGGGGAGCTTGAAGAGGATCAAATCAGAGGATTTGTGGGTGCTAACTTGCAGTGAAGATGTGGTTGACACTGAAAAAGTCTCCAAGGAAGATCTGTCACGGTTCTATCTCTATATAAGCAGCCTGCCAAAGGAATATCCAGACGCCCTTTTCTTAGTTTATTATGAATACTTGATTTGAACCAAACTAAACCAATTTCTGTACAGATCCCTTCATAGCCGACCGTCAAATGTAAACACTGAGTTGGGTGTCCAAAACTTGCTCCTTTTTATGAAATGAAATGAAATGGTTTACAACT

mRNA sequence

CAGACGCCGCTGTCCCTTCCTTATCGGTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGGGTGTTCTGTAAACACGTTCTTGAAGAAAAGTATTTGGTGGGTATATTATTTTTGGGGGTTTCGAAGTTGTGGGATTATGTATTATCGGCTATCGAGGTCTTCTTGTAAGGATTCTCTGAAAGTCCTCGAAGAAGATGTAAATCGCGCCAATGCTCTGGCTGTTGCAATTCCAAGAGCCAAACATGGTTCATATCTCCAAATGAAATTGGTTTACAGTCAATTAGCTCCACTGTTTCTGTTTTTGATGCAATGGGTGGATTGTTCTTGCACATGTGTTCTTCCCAGATACCTAAATTTCTTCCACATACTTGTTTACAAGGCGCGTCTCTCTTTTCTTCACTTCTTTAATGTGGCTTTGTTTTTTATGTTTATGTGTCTTAGTTTGTTCTGTCAAATGATGTTGTTCAGGTTTACACAGATGGAAGACCCAACCTCTGTGATATTGCCATCCCTTCAACGGCTCCAAGCGAACGCCAGGGAGTTGGATAACGCTAAAGAGAAGCGTGTGGGAGTCGAAGGAGATGGAAAGCTCTCCAATGGTGATGTAGAGAGAGAAGAAGAATGTGGGATTTGCTTAGAAGCTTCCACAAAGATGGTGTTGCCAAACTGCTGCCATTCAATGTGCATCAAATGCTACCACAATTGGAACACGAGATCGGAGTCGTGCCCGTTTTGTCGGGGGAGCTTGAAGAGGATCAAATCAGAGGATTTGTGGGTGCTAACTTGCAGTGAAGATGTGGTTGACACTGAAAAAGTCTCCAAGGAAGATCTGTCACGGTTCTATCTCTATATAAGCAGCCTGCCAAAGGAATATCCAGACGCCCTTTTCTTAGTTTATTATGAATACTTGATTTGAACCAAACTAAACCAATTTCTGTACAGATCCCTTCATAGCCGACCGTCAAATGTAAACACTGAGTTGGGTGTCCAAAACTTGCTCCTTTTTATGAAATGAAATGAAATGGTTTACAACT

Coding sequence (CDS)

ATGTATTATCGGCTATCGAGGTCTTCTTGTAAGGATTCTCTGAAAGTCCTCGAAGAAGATGTAAATCGCGCCAATGCTCTGGCTGTTGCAATTCCAAGAGCCAAACATGGTTCATATCTCCAAATGAAATTGGTTTACAGTCAATTAGCTCCACTGTTTCTGTTTTTGATGCAATGGGTGGATTGTTCTTGCACATGTGTTCTTCCCAGATACCTAAATTTCTTCCACATACTTGTTTACAAGGCGCGTCTCTCTTTTCTTCACTTCTTTAATGTGGCTTTGTTTTTTATGTTTATGTGTCTTAGTTTGTTCTGTCAAATGATGTTGTTCAGGTTTACACAGATGGAAGACCCAACCTCTGTGATATTGCCATCCCTTCAACGGCTCCAAGCGAACGCCAGGGAGTTGGATAACGCTAAAGAGAAGCGTGTGGGAGTCGAAGGAGATGGAAAGCTCTCCAATGGTGATGTAGAGAGAGAAGAAGAATGTGGGATTTGCTTAGAAGCTTCCACAAAGATGGTGTTGCCAAACTGCTGCCATTCAATGTGCATCAAATGCTACCACAATTGGAACACGAGATCGGAGTCGTGCCCGTTTTGTCGGGGGAGCTTGAAGAGGATCAAATCAGAGGATTTGTGGGTGCTAACTTGCAGTGAAGATGTGGTTGACACTGAAAAAGTCTCCAAGGAAGATCTGTCACGGTTCTATCTCTATATAAGCAGCCTGCCAAAGGAATATCCAGACGCCCTTTTCTTAGTTTATTATGAATACTTGATTTGA

Protein sequence

MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWVDCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTSVILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCHSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYISSLPKEYPDALFLVYYEYLI

Homology

[Close]

BLAST of Carg20474 vs. NCBI nr
Match: KAG7020116.1 (RING finger protein-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 518.8 bits (1335), Expect = 2.6e-143
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV
Sbjct: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS
Sbjct: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120

Query: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180
           VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH
Sbjct: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180

Query: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240
           SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS
Sbjct: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240

Query: 241 SLPKEYPDALFLVYYEYLI 260
           SLPKEYPDALFLVYYEYLI
Sbjct: 241 SLPKEYPDALFLVYYEYLI 259

[Close]

BLAST of Carg20474 vs. NCBI nr
Match: XP_022951171.1 (uncharacterized protein LOC111454098 [Cucurbita moschata])

HSP 1 Score: 430.3 bits (1105), Expect = 1.2e-116
Identity = 222/259 (85.71%), Postives = 226/259 (87.26%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV
Sbjct: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTCVLPRYLNFFHILVYK         +                   R   ++D  +
Sbjct: 61  DCSCTCVLPRYLNFFHILVYKVYTDGRPNLSAH----------------GRKATLKDFYA 120

Query: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180
           VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH
Sbjct: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180

Query: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240
           SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS
Sbjct: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240

Query: 241 SLPKEYPDALFLVYYEYLI 260
           SLPKEYPDALFLVYYEYLI
Sbjct: 241 SLPKEYPDALFLVYYEYLI 243

[Close]

BLAST of Carg20474 vs. NCBI nr
Match: XP_023536785.1 (uncharacterized protein LOC111798063 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.2 bits (1097), Expect = 1.0e-115
Identity = 220/259 (84.94%), Postives = 226/259 (87.26%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV
Sbjct: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTCVLPRYLNFFHILVYK         +                   R   ++D  +
Sbjct: 61  DCSCTCVLPRYLNFFHILVYKVYTDGRPNLSAH----------------GRKATLKDFYA 120

Query: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180
           VILPSLQRLQA+A+ELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH
Sbjct: 121 VILPSLQRLQASAKELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180

Query: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240
           SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS
Sbjct: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240

Query: 241 SLPKEYPDALFLVYYEYLI 260
           SLPKEYPDALFLVYYEYLI
Sbjct: 241 SLPKEYPDALFLVYYEYLI 243

[Close]

BLAST of Carg20474 vs. NCBI nr
Match: KAG6585198.1 (E3 ubiquitin-protein ligase AIRP2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 426.4 bits (1095), Expect = 1.8e-115
Identity = 221/259 (85.33%), Postives = 225/259 (86.87%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV
Sbjct: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTCVL RYLNFFHILVYK         +                   R   ++D  +
Sbjct: 61  DCSCTCVLLRYLNFFHILVYKVYTDGRPNLSAH----------------GRKATLKDFYA 120

Query: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180
           VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH
Sbjct: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180

Query: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240
           SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS
Sbjct: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240

Query: 241 SLPKEYPDALFLVYYEYLI 260
           SLPKEYPDALFLVYYEYLI
Sbjct: 241 SLPKEYPDALFLVYYEYLI 243

[Close]

BLAST of Carg20474 vs. NCBI nr
Match: XP_023002087.1 (uncharacterized protein LOC111496061 [Cucurbita maxima])

HSP 1 Score: 425.6 bits (1093), Expect = 3.0e-115
Identity = 219/259 (84.56%), Postives = 225/259 (86.87%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPL LFLMQWV
Sbjct: 111 MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLLLFLMQWV 170

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTCVLPRYLNFFHILVYK         +                   R   ++D  +
Sbjct: 171 DCSCTCVLPRYLNFFHILVYKVYTDGRPNLSAH----------------GRKATLKDFYA 230

Query: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180
           VILPSLQRLQANA+ELDNAKEKRVGV+GDGKLSNGDVEREEECGICLEASTKMVLPNCCH
Sbjct: 231 VILPSLQRLQANAKELDNAKEKRVGVQGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 290

Query: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240
           SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS
Sbjct: 291 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 350

Query: 241 SLPKEYPDALFLVYYEYLI 260
           SLPKEYPDALFLVYYEYLI
Sbjct: 351 SLPKEYPDALFLVYYEYLI 353

[Close]

BLAST of Carg20474 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.2e-45
Identity = 107/256 (41.80%), Postives = 135/256 (52.73%), Query Frame = 0


Query: 7   RSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWVDCSCTC 66
           R S KDSLK LE D+  AN LA   P    G Y+QM+L YS  A LFLFL+QW DC    
Sbjct: 2   RKSFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCH--- 61

Query: 67  VLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTSVILPSL 126
                L    IL+YKA +                       +  R T + +   V+ PSL
Sbjct: 62  -FAGALGLLRILIYKAYVD----------------GKTTMSLHERKTSIREFYDVLFPSL 121

Query: 127 QRLQANARELDNAKEKRV--------GVEGDGKLSNGDVEREEECGICLEASTKMVLPNC 186
            +L     +++  K+K +             GK+S  D+EREEECGICLE   K+VLP C
Sbjct: 122 LQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLEREEECGICLEIRNKVVLPTC 181

Query: 187 CHSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLY 246
            HSMCI CY NW  RS+SCPFCRGSLKR+ S DLW+ TCS ++ D   + KE+L R  +Y
Sbjct: 182 NHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWIYTCSAEIADLPAIYKENLKRLLIY 237

Query: 247 ISSLPKEYPDALFLVY 255
           I  LP    D   + Y
Sbjct: 242 IDKLPLVTSDPNLVPY 237

[Close]

BLAST of Carg20474 vs. ExPASy TrEMBL
Match: A0A6J1GGU1 (uncharacterized protein LOC111454098 OS=Cucurbita moschata OX=3662 GN=LOC111454098 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 5.9e-117
Identity = 222/259 (85.71%), Postives = 226/259 (87.26%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV
Sbjct: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTCVLPRYLNFFHILVYK         +                   R   ++D  +
Sbjct: 61  DCSCTCVLPRYLNFFHILVYKVYTDGRPNLSAH----------------GRKATLKDFYA 120

Query: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180
           VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH
Sbjct: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180

Query: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240
           SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS
Sbjct: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240

Query: 241 SLPKEYPDALFLVYYEYLI 260
           SLPKEYPDALFLVYYEYLI
Sbjct: 241 SLPKEYPDALFLVYYEYLI 243

[Close]

BLAST of Carg20474 vs. ExPASy TrEMBL
Match: A0A6J1KIH2 (uncharacterized protein LOC111496061 OS=Cucurbita maxima OX=3661 GN=LOC111496061 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.5e-115
Identity = 219/259 (84.56%), Postives = 225/259 (86.87%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPL LFLMQWV
Sbjct: 111 MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLLLFLMQWV 170

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTCVLPRYLNFFHILVYK         +                   R   ++D  +
Sbjct: 171 DCSCTCVLPRYLNFFHILVYKVYTDGRPNLSAH----------------GRKATLKDFYA 230

Query: 121 VILPSLQRLQANARELDNAKEKRVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 180
           VILPSLQRLQANA+ELDNAKEKRVGV+GDGKLSNGDVEREEECGICLEASTKMVLPNCCH
Sbjct: 231 VILPSLQRLQANAKELDNAKEKRVGVQGDGKLSNGDVEREEECGICLEASTKMVLPNCCH 290

Query: 181 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 240
           SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS
Sbjct: 291 SMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYIS 350

Query: 241 SLPKEYPDALFLVYYEYLI 260
           SLPKEYPDALFLVYYEYLI
Sbjct: 351 SLPKEYPDALFLVYYEYLI 353

[Close]

BLAST of Carg20474 vs. ExPASy TrEMBL
Match: A0A5A7V5Q1 (RING-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001750 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.6e-104
Identity = 202/267 (75.66%), Postives = 215/267 (80.52%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRL+ SSC DSLKVLEEDV RANALA AIPRAKHGSYLQMKLVY+QLAP FLFLMQW+
Sbjct: 1   MYYRLAMSSCADSLKVLEEDVRRANALAAAIPRAKHGSYLQMKLVYNQLAPFFLFLMQWL 60

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTC+LPRYLNFFHILVYKA LS        +                R   ++D  +
Sbjct: 61  DCSCTCILPRYLNFFHILVYKAGLSVYTDGRPNI------------SAHGRKATVKDFYA 120

Query: 121 VILPSLQRLQANARELDNAKEKRV--------GVEGDGKLSNGDVEREEECGICLEASTK 180
           VILPSLQRLQAN  E DNAKEK           ++GDGKLSNGD+ERE+ECGICLE STK
Sbjct: 121 VILPSLQRLQANVEEFDNAKEKHAKSDNSSERRIQGDGKLSNGDLEREDECGICLEPSTK 180

Query: 181 MVLPNCCHSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDL 240
           MVLPNCCHSMCIKCYHNWN RSESCPFCRGSLKRIKSEDLWVLTC+EDVVD EKVSKEDL
Sbjct: 181 MVLPNCCHSMCIKCYHNWNMRSESCPFCRGSLKRIKSEDLWVLTCNEDVVDAEKVSKEDL 240

Query: 241 SRFYLYISSLPKEYPDALFLVYYEYLI 260
           SRFYLYISSLPKEYPDALFLVYYEYLI
Sbjct: 241 SRFYLYISSLPKEYPDALFLVYYEYLI 255

[Close]

BLAST of Carg20474 vs. ExPASy TrEMBL
Match: A0A1S3BB06 (uncharacterized protein LOC103487741 OS=Cucumis melo OX=3656 GN=LOC103487741 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 7.5e-104
Identity = 200/267 (74.91%), Postives = 214/267 (80.15%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYYRL+ SSC DSLKVLEEDV RANALA AIPRAKHGSYLQMKLVY+QLAP FLFLMQW+
Sbjct: 1   MYYRLAMSSCADSLKVLEEDVRRANALAAAIPRAKHGSYLQMKLVYNQLAPFFLFLMQWL 60

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           DCSCTC+LPRYLNFFHILVYK         N++                 R   ++D  +
Sbjct: 61  DCSCTCILPRYLNFFHILVYKVYTDGRP--NISAH--------------GRKATVKDFYA 120

Query: 121 VILPSLQRLQANARELDNAKEKRV--------GVEGDGKLSNGDVEREEECGICLEASTK 180
           VILPSLQRLQAN  E DNAKEK           ++GDGKLSNGD+ERE+ECGICLE STK
Sbjct: 121 VILPSLQRLQANVEEFDNAKEKHAKSDNSSERRIQGDGKLSNGDLEREDECGICLEPSTK 180

Query: 181 MVLPNCCHSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDL 240
           MVLPNCCHSMCIKCYHNWN RSESCPFCRGSLKRIKSEDLWVLTC+EDVVD EKVSKEDL
Sbjct: 181 MVLPNCCHSMCIKCYHNWNMRSESCPFCRGSLKRIKSEDLWVLTCNEDVVDAEKVSKEDL 240

Query: 241 SRFYLYISSLPKEYPDALFLVYYEYLI 260
           SRFYLYISSLPKEYPDALFLVYYEYLI
Sbjct: 241 SRFYLYISSLPKEYPDALFLVYYEYLI 251

[Close]

BLAST of Carg20474 vs. ExPASy TrEMBL
Match: A0A6J1BQ55 (RING-H2 finger protein ATL47-like OS=Momordica charantia OX=3673 GN=LOC111004624 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.8e-103
Identity = 201/269 (74.72%), Postives = 218/269 (81.04%), Query Frame = 0


Query: 1   MYYRLSR--SSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQ 60
           MYYRL+R  SSCKDS+KVLEEDV RANALA AIPRAKHGSYLQMKLVY+ LAPLFLFLMQ
Sbjct: 1   MYYRLTRSSSSCKDSMKVLEEDVQRANALAAAIPRAKHGSYLQMKLVYNHLAPLFLFLMQ 60

Query: 61  WVDCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDP 120
           WVDCSCTC+LPRYLNFFHIL+YK         N++                 R   ++D 
Sbjct: 61  WVDCSCTCILPRYLNFFHILIYKVYTDGRP--NISAH--------------GRKATIKDF 120

Query: 121 TSVILPSLQRLQANARELDNAKEKRV--------GVEGDGKLSNGDVEREEECGICLEAS 180
            +VILPSLQRLQAN  ELD+A ++RV          +GDGKLSNGD+ERE+ECGICLE S
Sbjct: 121 YAVILPSLQRLQANVDELDSAIDRRVKADNSNDKRAQGDGKLSNGDLEREDECGICLEPS 180

Query: 181 TKMVLPNCCHSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKE 240
           TKMVLPNCCHSMCIKCYHNWNTRSESCPFCRGSL+RIKSEDLWVLTCSEDVVD EKVSKE
Sbjct: 181 TKMVLPNCCHSMCIKCYHNWNTRSESCPFCRGSLRRIKSEDLWVLTCSEDVVDAEKVSKE 240

Query: 241 DLSRFYLYISSLPKEYPDALFLVYYEYLI 260
           DLSRFYLYISSLPKEYPDALFLVYYEYLI
Sbjct: 241 DLSRFYLYISSLPKEYPDALFLVYYEYLI 253

[Close]

BLAST of Carg20474 vs. TAIR 10
Match: AT1G24440.1 (RING/U-box superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 6.5e-76
Identity = 148/269 (55.02%), Postives = 184/269 (68.40%), Query Frame = 0


Query: 1   MYYRLSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWV 60
           MYY+L++SS +DSLK+LE D+  AN LA  IP  K G  LQMKLV S LAP F+FL+QW+
Sbjct: 1   MYYQLTKSSYRDSLKILEADIEHANGLAAEIPMGKSGVRLQMKLVCSNLAPFFIFLLQWM 60

Query: 61  DCSCTCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTS 120
           D S  C+LPRY +FFHIL+YK R                    + +    R   + +   
Sbjct: 61  DFS--CLLPRYFDFFHILIYKVRAD----------------GRWNRSRYGRKATIREFYG 120

Query: 121 VILPSLQRLQANARELDNAK----------EKRVGVEGDGKLSNGDVEREEECGICLEAS 180
           VILPSL+RL  N  +L +            +K+  +EG   +++ D+ERE+ECGICLE  
Sbjct: 121 VILPSLERLHINFADLPDESLWYPNPKAITKKQYDIEGSRYMNSIDLEREDECGICLEPC 180

Query: 181 TKMVLPNCCHSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKE 240
           TKMVLPNCCH+MCIKCY NWNT+SESCPFCRGS+KR+ SEDLWVLTC EDVVD E V+KE
Sbjct: 181 TKMVLPNCCHAMCIKCYRNWNTKSESCPFCRGSIKRVNSEDLWVLTCDEDVVDPETVTKE 240

Query: 241 DLSRFYLYISSLPKEYPDALFLVYYEYLI 260
           DL RFYL+I+SLPK+YP+A FLVY EYLI
Sbjct: 241 DLLRFYLHINSLPKDYPEAAFLVYNEYLI 251

[Close]

BLAST of Carg20474 vs. TAIR 10
Match: AT1G13195.1 (RING/U-box superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 7.7e-69
Identity = 140/261 (53.64%), Postives = 169/261 (64.75%), Query Frame = 0


Query: 6   SRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWVDCSCT 65
           S SS  +SLKVLE DV  AN+LA AIP  K+   LQMKLV+S  A L LFL++W+D S +
Sbjct: 12  SSSSYYESLKVLEADVQHANSLAEAIPMGKNNVRLQMKLVHSNFASLLLFLLRWIDLSSS 71

Query: 66  CVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTSVILPS 125
           C++PRYLN FH+LVYK +                           R   + +   VILPS
Sbjct: 72  CLIPRYLNLFHVLVYKVQSDGQPKLTTH----------------GRKATISEFYGVILPS 131

Query: 126 LQRLQANARELDNAK--------EKRVGVEG-DGKLSNGDVEREEECGICLEASTKMVLP 185
           LQ L +N  EL+            K++  E    + SN  +EREEECGICLE  TKMVLP
Sbjct: 132 LQLLHSNLDELETTDIGFDLKRLSKKITKEARSSRFSNAGLEREEECGICLETCTKMVLP 191

Query: 186 NCCHSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFY 245
           NCCHSMCIKCY NWN +S+SCPFCRGS+KR+ SEDLWVL    DVVDT   S+EDL RFY
Sbjct: 192 NCCHSMCIKCYRNWNLKSQSCPFCRGSMKRVNSEDLWVLAGDNDVVDTRTASREDLFRFY 251

Query: 246 LYISSLPKEYPDALFLVYYEY 258
           LYI+SLPK+YP+ALF+VYYEY
Sbjct: 252 LYINSLPKDYPEALFVVYYEY 256

[Close]

BLAST of Carg20474 vs. TAIR 10
Match: AT1G13195.2 (RING/U-box superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 2.9e-60
Identity = 122/232 (52.59%), Postives = 149/232 (64.22%), Query Frame = 0


Query: 35  KHGSYLQMKLVYSQLAPLFLFLMQWVDCSCTCVLPRYLNFFHILVYKARLSFLHFFNVAL 94
           K+   LQMKLV+S  A L LFL++W+D S +C++PRYLN FH+LVYK +           
Sbjct: 3   KNNVRLQMKLVHSNFASLLLFLLRWIDLSSSCLIPRYLNLFHVLVYKVQSDGQPKLTTH- 62

Query: 95  FFMFMCLSLFCQMMLFRFTQMEDPTSVILPSLQRLQANARELDNAK--------EKRVGV 154
                           R   + +   VILPSLQ L +N  EL+            K++  
Sbjct: 63  ---------------GRKATISEFYGVILPSLQLLHSNLDELETTDIGFDLKRLSKKITK 122

Query: 155 EG-DGKLSNGDVEREEECGICLEASTKMVLPNCCHSMCIKCYHNWNTRSESCPFCRGSLK 214
           E    + SN  +EREEECGICLE  TKMVLPNCCHSMCIKCY NWN +S+SCPFCRGS+K
Sbjct: 123 EARSSRFSNAGLEREEECGICLETCTKMVLPNCCHSMCIKCYRNWNLKSQSCPFCRGSMK 182

Query: 215 RIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYISSLPKEYPDALFLVYYEY 258
           R+ SEDLWVL    DVVDT   S+EDL RFYLYI+SLPK+YP+ALF+VYYEY
Sbjct: 183 RVNSEDLWVLAGDNDVVDTRTASREDLFRFYLYINSLPKDYPEALFVVYYEY 218

[Close]

BLAST of Carg20474 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 184.9 bits (468), Expect = 8.3e-47
Identity = 107/256 (41.80%), Postives = 135/256 (52.73%), Query Frame = 0


Query: 7   RSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWVDCSCTC 66
           R S KDSLK LE D+  AN LA   P    G Y+QM+L YS  A LFLFL+QW DC    
Sbjct: 2   RKSFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCH--- 61

Query: 67  VLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTSVILPSL 126
                L    IL+YKA +                       +  R T + +   V+ PSL
Sbjct: 62  -FAGALGLLRILIYKAYVD----------------GKTTMSLHERKTSIREFYDVLFPSL 121

Query: 127 QRLQANARELDNAKEKRV--------GVEGDGKLSNGDVEREEECGICLEASTKMVLPNC 186
            +L     +++  K+K +             GK+S  D+EREEECGICLE   K+VLP C
Sbjct: 122 LQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLEREEECGICLEIRNKVVLPTC 181

Query: 187 CHSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLY 246
            HSMCI CY NW  RS+SCPFCRGSLKR+ S DLW+ TCS ++ D   + KE+L R  +Y
Sbjct: 182 NHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWIYTCSAEIADLPAIYKENLKRLLIY 237

Query: 247 ISSLPKEYPDALFLVY 255
           I  LP    D   + Y
Sbjct: 242 IDKLPLVTSDPNLVPY 237

[Close]

BLAST of Carg20474 vs. TAIR 10
Match: AT5G58787.1 (RING/U-box superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 2.4e-46
Identity = 100/252 (39.68%), Postives = 146/252 (57.94%), Query Frame = 0


Query: 5   LSRSSCKDSLKVLEEDVNRANALAVAIPRAKHGSYLQMKLVYSQLAPLFLFLMQWVDCSC 64
           +++ S KDSLK LE D+  AN LA+  PR K G+ +QM+L YS  A  FLFL+QW DC  
Sbjct: 1   MAKLSFKDSLKALEADIQHANTLALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCK- 60

Query: 65  TCVLPRYLNFFHILVYKARLSFLHFFNVALFFMFMCLSLFCQMMLFRFTQMEDPTSVILP 124
              L  +L    +L+Y                M          +  R   + +  +VILP
Sbjct: 61  ---LAGFLGLLRVLIY----------------MTYADGKTTMSVYERKASIREFQAVILP 120

Query: 125 SLQRLQANARELDNAKEK-----RVGVEGDGKLSNGDVEREEECGICLEASTKMVLPNCC 184
           SL +LQ    ++D++K+K     R   + + ++S  ++EREEECGIC+E ++K+VLPNC 
Sbjct: 121 SLSQLQRGVTDIDDSKQKEVCKMRYRKKDESEMSEIEIEREEECGICMEMNSKVVLPNCT 180

Query: 185 HSMCIKCYHNWNTRSESCPFCRGSLKRIKSEDLWVLTCSEDVVDTEKVSKEDLSRFYLYI 244
           HS+CIKCY +W  RS+SCPFCR SLKR+ S DLW+     D V+   +++E+  R ++YI
Sbjct: 181 HSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLDQNDTVNLTAIARENQKRLFMYI 232

Query: 245 SSLPKEYPDALF 252
             LP   PD ++
Sbjct: 241 EKLPLVVPDQVY 232

The following BLAST results are available for this feature:

BLAST of Carg20474 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020116.1	2.6e-143	100.00	RING finger protein-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022951171.1	1.2e-116	85.71	uncharacterized protein LOC111454098 [Cucurbita moschata]	[more]
XP_023536785.1	1.0e-115	84.94	uncharacterized protein LOC111798063 [Cucurbita pepo subsp. pepo]	[more]
KAG6585198.1	1.8e-115	85.33	E3 ubiquitin-protein ligase AIRP2, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023002087.1	3.0e-115	84.56	uncharacterized protein LOC111496061 [Cucurbita maxima]	[more]

BLAST of Carg20474 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M022	1.2e-45	41.80	E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ...	[more]

BLAST of Carg20474 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GGU1	5.9e-117	85.71	uncharacterized protein LOC111454098 OS=Cucurbita moschata OX=3662 GN=LOC1114540...	[more]
A0A6J1KIH2	1.5e-115	84.56	uncharacterized protein LOC111496061 OS=Cucurbita maxima OX=3661 GN=LOC111496061...	[more]
A0A5A7V5Q1	2.6e-104	75.66	RING-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A1S3BB06	7.5e-104	74.91	uncharacterized protein LOC103487741 OS=Cucumis melo OX=3656 GN=LOC103487741 PE=...	[more]
A0A6J1BQ55	4.8e-103	74.72	RING-H2 finger protein ATL47-like OS=Momordica charantia OX=3673 GN=LOC111004624...	[more]

BLAST of Carg20474 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24440.1	6.5e-76	55.02	RING/U-box superfamily protein	[more]
AT1G13195.1	7.7e-69	53.64	RING/U-box superfamily protein	[more]
AT1G13195.2	2.9e-60	52.59	RING/U-box superfamily protein	[more]
AT5G01520.1	8.3e-47	41.80	RING/U-box superfamily protein	[more]
AT5G58787.1	2.4e-46	39.68	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 163..200 e-value: 5.6E-5 score: 32.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 162..201 e-value: 1.1E-7 score: 32.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 163..201 score: 11.722122
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 133..212 e-value: 3.0E-13 score: 51.0
None	No IPR available	PANTHER	PTHR15315:SF35	F21J9.10	coord: 114..259
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 5..83
None	No IPR available	PANTHER	PTHR15315:SF35	F21J9.10	coord: 5..83
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 114..259
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 156..207
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 178..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20474-RA	Carg20474-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity