Carg20448 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20448
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nuclear factor Y, subunit C13
Location	Carg_Chr12: 309865 .. 310110 (-)
RNA-Seq Expression	Carg20448
Synteny	Carg20448

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGACGAGTGAAGATGATGATGAAGATCGATAAGGACATCGGTAAAGTGAATTCAGAAGCCCTGCTTATAGTCTCATGCGCCACCGAGGTCTTCCTCAAGCTTCTTGCTGAAAAATCTGCAGATGTCACCGCTGAGAAGAAACGGAAGATCGTGAAGCTTGAACACATCGGAATCATCGTCAAGAGGCATCGACTGATGAGTGATTTTCTCCTCGATTCGCTTCCTCTTCCATCTCAGCCGTAG

mRNA sequence

ATGGGACGAGTGAAGATGATGATGAAGATCGATAAGGACATCGGTAAAGTGAATTCAGAAGCCCTGCTTATAGTCTCATGCGCCACCGAGGTCTTCCTCAAGCTTCTTGCTGAAAAATCTGCAGATGTCACCGCTGAGAAGAAACGGAAGATCGTGAAGCTTGAACACATCGGAATCATCGTCAAGAGGCATCGACTGATGAGTGATTTTCTCCTCGATTCGCTTCCTCTTCCATCTCAGCCGTAG

Coding sequence (CDS)

ATGGGACGAGTGAAGATGATGATGAAGATCGATAAGGACATCGGTAAAGTGAATTCAGAAGCCCTGCTTATAGTCTCATGCGCCACCGAGGTCTTCCTCAAGCTTCTTGCTGAAAAATCTGCAGATGTCACCGCTGAGAAGAAACGGAAGATCGTGAAGCTTGAACACATCGGAATCATCGTCAAGAGGCATCGACTGATGAGTGATTTTCTCCTCGATTCGCTTCCTCTTCCATCTCAGCCGTAG

Protein sequence

MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGIIVKRHRLMSDFLLDSLPLPSQP

Homology

[Close]

BLAST of Carg20448 vs. NCBI nr
Match: KAG7020142.1 (hypothetical protein SDJN02_16824, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 153.3 bits (386), Expect = 9.0e-34
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0


Query: 1  MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
          MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII
Sbjct: 1  MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60

Query: 61 VKRHRLMSDFLLDSLPLPSQP 82
          VKRHRLMSDFLLDSLPLPSQP
Sbjct: 61 VKRHRLMSDFLLDSLPLPSQP 81

[Close]

BLAST of Carg20448 vs. NCBI nr
Match: KAA0064731.1 (dr1-associated corepressor [Cucumis melo var. makuwa])

HSP 1 Score: 129.0 bits (323), Expect = 1.8e-26
Identity = 67/80 (83.75%), Postives = 73/80 (91.25%), Query Frame = 0


Query: 2  GRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGIIV 61
          GRVK +MK+DKDIGKVNSEAL IVSCATE+FLKLLAEKSA+V AEKKRK VKLEHI + V
Sbjct: 17 GRVKKIMKLDKDIGKVNSEALFIVSCATELFLKLLAEKSAEVAAEKKRKTVKLEHIRMAV 76

Query: 62 KRHRLMSDFLLDSLPLPSQP 82
          KRHR +SDFLLDSLPLPSQP
Sbjct: 77 KRHRSISDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. NCBI nr
Match: XP_008445504.1 (PREDICTED: dr1-associated corepressor [Cucumis melo])

HSP 1 Score: 128.3 bits (321), Expect = 3.1e-26
Identity = 66/80 (82.50%), Postives = 73/80 (91.25%), Query Frame = 0


Query: 2  GRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGIIV 61
          GRVK +MK+DKDIGKVNSEAL +VSCATE+FLKLLAEKSA+V AEKKRK VKLEHI + V
Sbjct: 17 GRVKKIMKLDKDIGKVNSEALFLVSCATELFLKLLAEKSAEVAAEKKRKTVKLEHIRMAV 76

Query: 62 KRHRLMSDFLLDSLPLPSQP 82
          KRHR +SDFLLDSLPLPSQP
Sbjct: 77 KRHRSISDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. NCBI nr
Match: XP_022997291.1 (DNA polymerase epsilon subunit C [Cucurbita maxima])

HSP 1 Score: 127.5 bits (319), Expect = 5.3e-26
Identity = 65/81 (80.25%), Postives = 74/81 (91.36%), Query Frame = 0


Query: 1  MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
          MGR+K +MK+DKDIGKVNSEAL +VSCATE+FL+LLAEKSA+V AEKKRK VKLEHI I 
Sbjct: 16 MGRIKKIMKLDKDIGKVNSEALFLVSCATELFLELLAEKSAEVAAEKKRKTVKLEHIRIA 75

Query: 61 VKRHRLMSDFLLDSLPLPSQP 82
          VKRH+ +SDFLLDSLPLPSQP
Sbjct: 76 VKRHQSISDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. NCBI nr
Match: KAG6598611.1 (Dr1-associated corepressor, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029547.1 Dr1-associated corepressor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 126.3 bits (316), Expect = 1.2e-25
Identity = 64/81 (79.01%), Postives = 74/81 (91.36%), Query Frame = 0


Query: 1  MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
          MGR+K +MK+DKDIGKVNSEAL +VSCATE+FL+LLAEKS++V AEKKRK VKLEHI I 
Sbjct: 16 MGRIKKIMKLDKDIGKVNSEALFLVSCATELFLELLAEKSSEVAAEKKRKTVKLEHIRIA 75

Query: 61 VKRHRLMSDFLLDSLPLPSQP 82
          VKRH+ +SDFLLDSLPLPSQP
Sbjct: 76 VKRHQSISDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. ExPASy TrEMBL
Match: A0A5A7VG98 (Dr1-associated corepressor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00350 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.8e-27
Identity = 67/80 (83.75%), Postives = 73/80 (91.25%), Query Frame = 0


Query: 2  GRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGIIV 61
          GRVK +MK+DKDIGKVNSEAL IVSCATE+FLKLLAEKSA+V AEKKRK VKLEHI + V
Sbjct: 17 GRVKKIMKLDKDIGKVNSEALFIVSCATELFLKLLAEKSAEVAAEKKRKTVKLEHIRMAV 76

Query: 62 KRHRLMSDFLLDSLPLPSQP 82
          KRHR +SDFLLDSLPLPSQP
Sbjct: 77 KRHRSISDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. ExPASy TrEMBL
Match: A0A1S3BDL4 (dr1-associated corepressor OS=Cucumis melo OX=3656 GN=LOC103488501 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.5e-26
Identity = 66/80 (82.50%), Postives = 73/80 (91.25%), Query Frame = 0


Query: 2  GRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGIIV 61
          GRVK +MK+DKDIGKVNSEAL +VSCATE+FLKLLAEKSA+V AEKKRK VKLEHI + V
Sbjct: 17 GRVKKIMKLDKDIGKVNSEALFLVSCATELFLKLLAEKSAEVAAEKKRKTVKLEHIRMAV 76

Query: 62 KRHRLMSDFLLDSLPLPSQP 82
          KRHR +SDFLLDSLPLPSQP
Sbjct: 77 KRHRSISDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. ExPASy TrEMBL
Match: A0A6J1K737 (DNA polymerase epsilon subunit C OS=Cucurbita maxima OX=3661 GN=LOC111492238 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.6e-26
Identity = 65/81 (80.25%), Postives = 74/81 (91.36%), Query Frame = 0


Query: 1  MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
          MGR+K +MK+DKDIGKVNSEAL +VSCATE+FL+LLAEKSA+V AEKKRK VKLEHI I 
Sbjct: 16 MGRIKKIMKLDKDIGKVNSEALFLVSCATELFLELLAEKSAEVAAEKKRKTVKLEHIRIA 75

Query: 61 VKRHRLMSDFLLDSLPLPSQP 82
          VKRH+ +SDFLLDSLPLPSQP
Sbjct: 76 VKRHQSISDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. ExPASy TrEMBL
Match: A0A0A0KGV1 (CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G365140 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 7.4e-26
Identity = 65/80 (81.25%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 2  GRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGIIV 61
          GRVK +MK+DKDIGKVNSEAL +VSCATE+FLKLLAEKSA+  AEKKRK VKLEHI + V
Sbjct: 17 GRVKKIMKLDKDIGKVNSEALFLVSCATELFLKLLAEKSAESAAEKKRKTVKLEHIRMAV 76

Query: 62 KRHRLMSDFLLDSLPLPSQP 82
          KRHR +SDFLLDSLPLPSQP
Sbjct: 77 KRHRSISDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. ExPASy TrEMBL
Match: A0A6J1HCT3 (DNA polymerase epsilon subunit C OS=Cucurbita moschata OX=3662 GN=LOC111462901 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-25
Identity = 63/81 (77.78%), Postives = 73/81 (90.12%), Query Frame = 0


Query: 1  MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
          MGR+K +MK+DKDIGKVNSEAL +VSCATE+FL+LLAEKS++V AEKKRK VKLEHI I 
Sbjct: 16 MGRIKKIMKLDKDIGKVNSEALFLVSCATELFLELLAEKSSEVAAEKKRKTVKLEHIRIA 75

Query: 61 VKRHRLMSDFLLDSLPLPSQP 82
          VKRH+ + DFLLDSLPLPSQP
Sbjct: 76 VKRHQSIGDFLLDSLPLPSQP 96

[Close]

BLAST of Carg20448 vs. TAIR 10
Match: AT5G43250.1 (nuclear factor Y, subunit C13 )

HSP 1 Score: 107.5 bits (267), Expect = 5.3e-24
Identity = 55/81 (67.90%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 1  MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
          +GRVK +MK+DKDI K+NSEAL +++ +TE+FL  LAEKSA VTAEKKRK V L+H+ I 
Sbjct: 14 IGRVKKIMKLDKDINKINSEALHVITYSTELFLHFLAEKSAVVTAEKKRKTVNLDHLRIA 73

Query: 61 VKRHRLMSDFLLDSLPLPSQP 82
          VKRH+  SDFLLDSLPLP+QP
Sbjct: 74 VKRHQPTSDFLLDSLPLPAQP 94

[Close]

BLAST of Carg20448 vs. TAIR 10
Match: AT1G08970.4 (nuclear factor Y, subunit C9 )

HSP 1 Score: 42.0 bits (97), Expect = 2.7e-04
Identity = 25/76 (32.89%), Postives = 46/76 (60.53%), Query Frame = 0


Query: 1   MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
           + R+K +MK D+D+  +++EA ++ + A E+F+  L  +S + T E KR+ ++   I   
Sbjct: 83  LARIKKIMKADEDVRMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAA 142

Query: 61  VKRHRLMSDFLLDSLP 77
           V R  +  DFL+D +P
Sbjct: 143 VTRTDIF-DFLVDIVP 157

[Close]

BLAST of Carg20448 vs. TAIR 10
Match: AT1G54830.2 (nuclear factor Y, subunit C3 )

HSP 1 Score: 42.0 bits (97), Expect = 2.7e-04
Identity = 25/76 (32.89%), Postives = 46/76 (60.53%), Query Frame = 0


Query: 1   MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
           + R+K +MK D+D+  +++EA ++ + A E+F+  L  +S + T E KR+ ++   I   
Sbjct: 73  LARIKKIMKADEDVRMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAA 132

Query: 61  VKRHRLMSDFLLDSLP 77
           V R  +  DFL+D +P
Sbjct: 133 VTRTDIF-DFLVDIVP 147

[Close]

BLAST of Carg20448 vs. TAIR 10
Match: AT1G08970.1 (nuclear factor Y, subunit C9 )

HSP 1 Score: 42.0 bits (97), Expect = 2.7e-04
Identity = 25/76 (32.89%), Postives = 46/76 (60.53%), Query Frame = 0


Query: 1   MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
           + R+K +MK D+D+  +++EA ++ + A E+F+  L  +S + T E KR+ ++   I   
Sbjct: 83  LARIKKIMKADEDVRMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAA 142

Query: 61  VKRHRLMSDFLLDSLP 77
           V R  +  DFL+D +P
Sbjct: 143 VTRTDIF-DFLVDIVP 157

[Close]

BLAST of Carg20448 vs. TAIR 10
Match: AT1G08970.3 (nuclear factor Y, subunit C9 )

HSP 1 Score: 42.0 bits (97), Expect = 2.7e-04
Identity = 25/76 (32.89%), Postives = 46/76 (60.53%), Query Frame = 0


Query: 1   MGRVKMMMKIDKDIGKVNSEALLIVSCATEVFLKLLAEKSADVTAEKKRKIVKLEHIGII 60
           + R+K +MK D+D+  +++EA ++ + A E+F+  L  +S + T E KR+ ++   I   
Sbjct: 83  LARIKKIMKADEDVRMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAA 142

Query: 61  VKRHRLMSDFLLDSLP 77
           V R  +  DFL+D +P
Sbjct: 143 VTRTDIF-DFLVDIVP 157

The following BLAST results are available for this feature:

BLAST of Carg20448 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020142.1	9.0e-34	100.00	hypothetical protein SDJN02_16824, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAA0064731.1	1.8e-26	83.75	dr1-associated corepressor [Cucumis melo var. makuwa]	[more]
XP_008445504.1	3.1e-26	82.50	PREDICTED: dr1-associated corepressor [Cucumis melo]	[more]
XP_022997291.1	5.3e-26	80.25	DNA polymerase epsilon subunit C [Cucurbita maxima]	[more]
KAG6598611.1	1.2e-25	79.01	Dr1-associated corepressor, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]

BLAST of Carg20448 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg20448 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VG98	8.8e-27	83.75	Dr1-associated corepressor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S3BDL4	1.5e-26	82.50	dr1-associated corepressor OS=Cucumis melo OX=3656 GN=LOC103488501 PE=4 SV=1	[more]
A0A6J1K737	2.6e-26	80.25	DNA polymerase epsilon subunit C OS=Cucurbita maxima OX=3661 GN=LOC111492238 PE=...	[more]
A0A0A0KGV1	7.4e-26	81.25	CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G3651...	[more]
A0A6J1HCT3	1.7e-25	77.78	DNA polymerase epsilon subunit C OS=Cucurbita moschata OX=3662 GN=LOC111462901 P...	[more]

BLAST of Carg20448 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43250.1	5.3e-24	67.90	nuclear factor Y, subunit C13	[more]
AT1G08970.4	2.7e-04	32.89	nuclear factor Y, subunit C9	[more]
AT1G54830.2	2.7e-04	32.89	nuclear factor Y, subunit C3	[more]
AT1G08970.1	2.7e-04	32.89	nuclear factor Y, subunit C9	[more]
AT1G08970.3	2.7e-04	32.89	nuclear factor Y, subunit C9	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 1..76 e-value: 9.1E-16 score: 60.1
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 8..76
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 1..57 e-value: 3.1E-13 score: 49.8
None	No IPR available	PANTHER	PTHR10252	HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED	coord: 1..81
None	No IPR available	PANTHER	PTHR10252:SF95	NUCLEAR FACTOR Y, SUBUNIT C13	coord: 1..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20448-RA	Carg20448-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046982	protein heterodimerization activity