Carg20389 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg20389 vs. NCBI nr
Match: KAG6607091.1 (putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036780.1 putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 702.2 bits (1811), Expect = 2.2e-198
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS
Sbjct: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300
           DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of Carg20389 vs. NCBI nr
Match: XP_022948283.1 (probable carboxylesterase 17 [Cucurbita moschata])

HSP 1 Score: 693.7 bits (1789), Expect = 7.8e-196
Identity = 338/344 (98.26%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITN IEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANI+YNTATR+GHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA+
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300
           DTYWRLSLPPTANRDHPWSNPLVNG AKLKEMKLPPLMV ISELDILLDRNLEFCAAMAG
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of Carg20389 vs. NCBI nr
Match: XP_023523365.1 (probable carboxylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 689.1 bits (1777), Expect = 1.9e-194
Identity = 336/344 (97.67%), Postives = 338/344 (98.26%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAAMY+DPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV
Sbjct: 6   MAAMYVDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITN IEHKWW GNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWSGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANI YNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS
Sbjct: 186 ANIVYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300
           DTYWRLSLPPTANRDHPWSNPLVNG AKLKE KLPPLMV ISELDILLDRNLEFCAAMAG
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKETKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKV+RVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 306 AGKKVDRVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of Carg20389 vs. NCBI nr
Match: XP_022998605.1 (probable carboxylesterase 17 [Cucurbita maxima])

HSP 1 Score: 684.9 bits (1766), Expect = 3.6e-193
Identity = 335/344 (97.38%), Postives = 338/344 (98.26%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAAMYIDPRLKLQMAKPHLQNG TSEEIEGLIRVYADGHVERPPIIPNVACSTTAS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAY+DGFNA+MWLKQQAITN IEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYNTATRLGHNESTLLKP TLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300
           DTYWRLSLPPTANRDHPWSNPLVNG AKLKEMKLPPLMV ISELDILLDRNLEFCAAMA 
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of Carg20389 vs. NCBI nr
Match: XP_022155020.1 (probable carboxylesterase 17 [Momordica charantia])

HSP 1 Score: 590.5 bits (1521), Expect = 9.4e-165
Identity = 291/343 (84.84%), Postives = 307/343 (89.50%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAA+ IDPRLKLQM K HLQ   T+EEIEGLIRVYADGHVERPPIIPNV CSTTAS+KGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
            ILSINYRLSPENRLPAAY+DGFNALMWLKQQAI+   EHKWWLGNCNPS LFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYN ATR G NE   LKPLTLKG+ILIQPFFGGESRTKSEKLAA P STSALTL+ +
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQP-STSALTLATA 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300
           DTYWRLSLPP A+RDHPWSNPLVNG  KL++ KLPP MV ISELDIL+DRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 344
           AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 306 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of Carg20389 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 9.0e-94
Identity = 181/330 (54.85%), Postives = 224/330 (67.88%), Query Frame = 0

Query: 18  HLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARI 77
           H ++G   EEIEGLI+V+ DG VERPPI+P V+ +   S K  TA D  I +  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFD--IKLSNDTWTRV 81

Query: 78  YIPNSQR-----NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPE 137
           YIP++        LPLLVYFHGGGFCVGSAAWSCYH+FL +LA KA+CVI+S+NYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 138 NRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLG 197
           +RLPAAYDDG N + WL +Q I+    +  WL  CN S +FLAGDSAGANIAY  A R+ 
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI- 201

Query: 198 HNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTA 257
                    L LKGIILI PFFGGESRT SEK     + +SALTLSASD YWRL+LP  A
Sbjct: 202 MASGKYANTLHLKGIILIHPFFGGESRTSSEK-QQHHTKSSALTLSASDAYWRLALPRGA 261

Query: 258 NRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAGAGKKVERVILKG 317
           +RDHPW NPL++ +      KLP  MV ++E DIL +RNLE C  M   GK+VE ++  G
Sbjct: 262 SRDHPWCNPLMSSAG----AKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 321

Query: 318 VGHGFQILNKDQISTQRTQELMGSIRAFIH 343
           VGH F IL+   +S  R  ++M  +  FIH
Sbjct: 322 VGHAFHILDNSSVSRDRIHDMMCRLHNFIH 342

BLAST of Carg20389 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.3e-81
Identity = 155/327 (47.40%), Postives = 214/327 (65.44%), Query Frame = 0

Query: 21  NGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARIYIP 80
           +G   +E+EGLI+VY DGHVER  ++P V  S    + GVT  DV+ID  TN WAR+Y+P
Sbjct: 20  HGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVP 79

Query: 81  -----NSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRL 140
                +S   LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++S+NYRL+PEN L
Sbjct: 80  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 139

Query: 141 PAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLGHNE 200
           PAAY+DG NA++WL +    N      W   C+  ++FLAGDSAG NIA   A RL   E
Sbjct: 140 PAAYEDGVNAILWLNKARNDNL-----WAKQCDFGRIFLAGDSAGGNIAQQVAARLASPE 199

Query: 201 STLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRD 260
              LK   ++G ILIQPF+ GE RT+SE+       T+ LTL++SD +WR+SLP  ANR+
Sbjct: 200 DLALK---IEGTILIQPFYSGEERTESERRVGN-DKTAVLTLASSDAWWRMSLPRGANRE 259

Query: 261 HPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAGAGKKVERVILKGVGH 320
           HP+  P+      +K   +   +V ++E+D+L+D N+E C    G    ++RV+ KGVGH
Sbjct: 260 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMC---DGNEDVIKRVLHKGVGH 319

Query: 321 GFQILNKDQISTQRTQELMGSIRAFIH 343
            F IL K Q++   T E++  I AFIH
Sbjct: 320 AFHILGKSQLAHTTTLEMLCQIDAFIH 330

BLAST of Carg20389 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 111/299 (37.12%), Postives = 172/299 (57.53%), Query Frame = 0

Query: 25  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASDKGVTAKDVLIDIFTNTWARIYIPNSQ 84
           +E+  GL+++ ++G V R   I  +       +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYD 144
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA++
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAITNAIEHKWWLG-NCNPSKLFLAGDSAGANIAYNTATRLGHNESTLL 204
           D    L WL  QA+++ + H +  G + +  ++F+ GDS+G NIA+  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWS 264
            P+ ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG-AGKKVERVILKGVGHGF 318
           NP    S  L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Carg20389 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.1e-42
Identity = 111/306 (36.27%), Postives = 155/306 (50.65%), Query Frame = 0

Query: 14  MAKPHLQNGGTSEEIEGLIR-----VYADGHVERPPIIPNVACS-TTASDKGVTAKDVLI 73
           M+  HL+  G+S+    L++     +  D  + RP  IP+ A S    S   V  KD+ +
Sbjct: 1   MSNDHLETTGSSDPNTNLLKYLPIVLNPDRTITRPIQIPSTAASPDPTSSSPVLTKDLAL 60

Query: 74  DIFTNTWARIYIPN----SQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVIL 133
           +   NT+ R+++P     +   LPL+VYFHGGGF + SAA + +H+F   +A  A  VI 
Sbjct: 61  NPLHNTFVRLFLPRHALYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIA 120

Query: 134 SINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAGANI 193
           S++YRL+PE+RLPAAYDD   AL W+K          +W     + S  F+ G+SAG NI
Sbjct: 121 SVDYRLAPEHRLPAAYDDAMEALQWIKDS------RDEWLTNFADFSNCFIMGESAGGNI 180

Query: 194 AYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTY 253
           AY+   R       LL PL +KG++L +P FGG  RT SE   A  S      L   D  
Sbjct: 181 AYHAGLRAAAVADELL-PLKIKGLVLDEPGFGGSKRTGSELRLANDSRLPTFVL---DLI 240

Query: 254 WRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPL----MVSISELDILLDRNLEFCAAMA 306
           W LSLP  A+RDH + NP           K+  L    MV     D ++DR +E    + 
Sbjct: 241 WELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLE 296

BLAST of Carg20389 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.5e-40
Identity = 110/321 (34.27%), Postives = 166/321 (51.71%), Query Frame = 0

Query: 37  DGHVERPPIIPNVACSTTASDKG----VTAKDVLIDIFTNTWARIYIPNSQRN------- 96
           DG + R   + N  C+    D        +KD+ ++   +TW R+Y+P+S  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 97  -LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYDDGFN 156
            LP++VY+HGGGF + S     +H+F   +A     +++S +YRL+PE+RLPAAYDDG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 157 ALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLGHNESTLLKPLTL 216
           AL W+K        + +W   + + S +FL G SAG N+AYN   R   + S  L PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSD-LSPLQI 200

Query: 217 KGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWSNPLV- 276
           +G+IL  PFFGGE R++SE           +    +D  W LSLP   +RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESEIRLMNDQVCPPI---VTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 277 NGSAKLKE---MKLPPLMVSISELDILLDRNLEFCAAMAGAGKKVERVILKGVGHGFQIL 336
           +GS KL++   ++   +M+   E D ++D   +    M   G +V      G  HG +I 
Sbjct: 261 DGSEKLEKIGRLRWKVMMIG-GEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIR 320

Query: 337 NKDQISTQRTQELMGSIRAFI 342
           +  +  T     L  SI+ FI
Sbjct: 321 DPSKRKT-----LFLSIKNFI 323

BLAST of Carg20389 vs. ExPASy TrEMBL
Match: A0A6J1G8X8 (probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 3.8e-196
Identity = 338/344 (98.26%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITN IEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANI+YNTATR+GHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA+
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300
           DTYWRLSLPPTANRDHPWSNPLVNG AKLKEMKLPPLMV ISELDILLDRNLEFCAAMAG
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of Carg20389 vs. ExPASy TrEMBL
Match: A0A6J1KCZ3 (probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 1.8e-193
Identity = 335/344 (97.38%), Postives = 338/344 (98.26%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAAMYIDPRLKLQMAKPHLQNG TSEEIEGLIRVYADGHVERPPIIPNVACSTTAS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAY+DGFNA+MWLKQQAITN IEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYNTATRLGHNESTLLKP TLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300
           DTYWRLSLPPTANRDHPWSNPLVNG AKLKEMKLPPLMV ISELDILLDRNLEFCAAMA 
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of Carg20389 vs. ExPASy TrEMBL
Match: A0A6J1DL90 (probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.5e-165
Identity = 291/343 (84.84%), Postives = 307/343 (89.50%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAA+ IDPRLKLQM K HLQ   T+EEIEGLIRVYADGHVERPPIIPNV CSTTAS+KGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
            ILSINYRLSPENRLPAAY+DGFNALMWLKQQAI+   EHKWWLGNCNPS LFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYN ATR G NE   LKPLTLKG+ILIQPFFGGESRTKSEKLAA P STSALTL+ +
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQP-STSALTLATA 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300
           DTYWRLSLPP A+RDHPWSNPLVNG  KL++ KLPP MV ISELDIL+DRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 344
           AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 306 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of Carg20389 vs. ExPASy TrEMBL
Match: A0A2N9EQ59 (Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4850 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.6e-130
Identity = 230/346 (66.47%), Postives = 274/346 (79.19%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAA+ +DPR KLQ+ K H Q+GG  EEIEGLI+V  DGHVERPPI+P V C T A ++GV
Sbjct: 1   MAAISLDPRFKLQVGKNHHQHGGVVEEIEGLIKVCMDGHVERPPIVPIVPC-TMALERGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TA DV+ID  T+ WARIY+P+    LPLLVYFHGGGFCVGSAAWSCY+EFL NLASKA C
Sbjct: 61  TANDVVIDKCTSLWARIYVPSCSGKLPLLVYFHGGGFCVGSAAWSCYNEFLSNLASKANC 120

Query: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180
           VI+S+NYRL+PENRLPAAY+DGFN LMW+KQQA+  + EHKWWL +CN + +FL GDS+G
Sbjct: 121 VIISVNYRLAPENRLPAAYEDGFNTLMWVKQQALNGSNEHKWWLRHCNLTSMFLGGDSSG 180

Query: 181 ANIAYNTATRLGH---NESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTL 240
           ANIAYN ATRLG    +EST+++PL LKG ILIQPFFGGE+RT SEK    P++ S LTL
Sbjct: 181 ANIAYNVATRLGSSGTSESTIIRPLCLKGTILIQPFFGGEARTWSEKYTNQPAN-SGLTL 240

Query: 241 SASDTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAA 300
             SDTYWRLSLP   NRD+PW NPL NG AKL+E +LP +MV +SE DIL DRNLEFC A
Sbjct: 241 LVSDTYWRLSLPIGTNRDNPWCNPLANGMAKLREFRLPSIMVCVSETDILKDRNLEFCTA 300

Query: 301 MAGAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 344
           +  AGK+VE VI KGVGH FQIL+  Q+S  RT E+M  I+AFI++
Sbjct: 301 LTNAGKRVETVIYKGVGHAFQILHNSQLSQPRTHEMMAHIKAFINE 344

BLAST of Carg20389 vs. ExPASy TrEMBL
Match: F6HSF1 (Abhydrolase_3 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s0006g03180 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.6e-128
Identity = 229/344 (66.57%), Postives = 270/344 (78.49%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60
           MAA+ +DPRL LQM K   QNG   E++EGLIRVY DGHVERP I+PNV C T A + GV
Sbjct: 1   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPC-TVALELGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQR-NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAK 120
           T KDV+I+ ++N WAR Y+P+     LPLLVYFHGGGFCVGSAAW+CYH FL +LASKA 
Sbjct: 61  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 120

Query: 121 CVILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSA 180
           C+I+S+NYRL+PENRLPAAY+DGFNA+MW+K QA+  A E KWWL  CN S LFL GDSA
Sbjct: 121 CLIMSVNYRLAPENRLPAAYEDGFNAVMWVKNQALNGAGEQKWWLSRCNLSSLFLTGDSA 180

Query: 181 GANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA 240
           GANIAYN ATRLG +++T LKPL+LKG ILIQPFFGGE+RT SE  +  P + SALTLSA
Sbjct: 181 GANIAYNVATRLGSSDTTFLKPLSLKGTILIQPFFGGEARTGSENHSTQPPN-SALTLSA 240

Query: 241 SDTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMA 300
           SDTYWRLSLP  ANRDHP  NPL NGS KL+ ++LPP MV IS+ DIL DRNL+FC AMA
Sbjct: 241 SDTYWRLSLPLGANRDHPCCNPLANGSTKLRTLQLPPTMVCISDTDILKDRNLQFCTAMA 300

Query: 301 GAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 344
            AGK++E VI KGVGH FQ+L    +S  RT+E++  IRAFI Q
Sbjct: 301 NAGKRLETVIYKGVGHAFQVLQNSDLSQPRTKEMISHIRAFITQ 342

BLAST of Carg20389 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 345.1 bits (884), Expect = 6.4e-95
Identity = 181/330 (54.85%), Postives = 224/330 (67.88%), Query Frame = 0

Query: 18  HLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARI 77
           H ++G   EEIEGLI+V+ DG VERPPI+P V+ +   S K  TA D  I +  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFD--IKLSNDTWTRV 81

Query: 78  YIPNSQR-----NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPE 137
           YIP++        LPLLVYFHGGGFCVGSAAWSCYH+FL +LA KA+CVI+S+NYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 138 NRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLG 197
           +RLPAAYDDG N + WL +Q I+    +  WL  CN S +FLAGDSAGANIAY  A R+ 
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI- 201

Query: 198 HNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTA 257
                    L LKGIILI PFFGGESRT SEK     + +SALTLSASD YWRL+LP  A
Sbjct: 202 MASGKYANTLHLKGIILIHPFFGGESRTSSEK-QQHHTKSSALTLSASDAYWRLALPRGA 261

Query: 258 NRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAGAGKKVERVILKG 317
           +RDHPW NPL++ +      KLP  MV ++E DIL +RNLE C  M   GK+VE ++  G
Sbjct: 262 SRDHPWCNPLMSSAG----AKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 321

Query: 318 VGHGFQILNKDQISTQRTQELMGSIRAFIH 343
           VGH F IL+   +S  R  ++M  +  FIH
Sbjct: 322 VGHAFHILDNSSVSRDRIHDMMCRLHNFIH 342

BLAST of Carg20389 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 9.5e-83
Identity = 155/327 (47.40%), Postives = 214/327 (65.44%), Query Frame = 0

Query: 21  NGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGVTAKDVLIDIFTNTWARIYIP 80
           +G   +E+EGLI+VY DGHVER  ++P V  S    + GVT  DV+ID  TN WAR+Y+P
Sbjct: 20  HGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVP 79

Query: 81  -----NSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRL 140
                +S   LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++S+NYRL+PEN L
Sbjct: 80  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 139

Query: 141 PAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLGHNE 200
           PAAY+DG NA++WL +    N      W   C+  ++FLAGDSAG NIA   A RL   E
Sbjct: 140 PAAYEDGVNAILWLNKARNDNL-----WAKQCDFGRIFLAGDSAGGNIAQQVAARLASPE 199

Query: 201 STLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRD 260
              LK   ++G ILIQPF+ GE RT+SE+       T+ LTL++SD +WR+SLP  ANR+
Sbjct: 200 DLALK---IEGTILIQPFYSGEERTESERRVGN-DKTAVLTLASSDAWWRMSLPRGANRE 259

Query: 261 HPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAGAGKKVERVILKGVGH 320
           HP+  P+      +K   +   +V ++E+D+L+D N+E C    G    ++RV+ KGVGH
Sbjct: 260 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMC---DGNEDVIKRVLHKGVGH 319

Query: 321 GFQILNKDQISTQRTQELMGSIRAFIH 343
            F IL K Q++   T E++  I AFIH
Sbjct: 320 AFHILGKSQLAHTTTLEMLCQIDAFIH 330

BLAST of Carg20389 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 9.0e-49
Identity = 111/299 (37.12%), Postives = 172/299 (57.53%), Query Frame = 0

Query: 25  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASDKGVTAKDVLIDIFTNTWARIYIPNSQ 84
           +E+  GL+++ ++G V R   I  +       +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYD 144
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA++
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAITNAIEHKWWLG-NCNPSKLFLAGDSAGANIAYNTATRLGHNESTLL 204
           D    L WL  QA+++ + H +  G + +  ++F+ GDS+G NIA+  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWS 264
            P+ ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG-AGKKVERVILKGVGHGF 318
           NP    S  L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Carg20389 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 9.0e-49
Identity = 111/299 (37.12%), Postives = 172/299 (57.53%), Query Frame = 0

Query: 25  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASDKGVTAKDVLIDIFTNTWARIYIPNSQ 84
           +E+  GL+++ ++G V R   I  +       +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYD 144
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA++
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAITNAIEHKWWLG-NCNPSKLFLAGDSAGANIAYNTATRLGHNESTLL 204
           D    L WL  QA+++ + H +  G + +  ++F+ GDS+G NIA+  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWS 264
            P+ ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG-AGKKVERVILKGVGHGF 318
           NP    S  L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Carg20389 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 168.3 bits (425), Expect = 1.1e-41
Identity = 110/321 (34.27%), Postives = 166/321 (51.71%), Query Frame = 0

Query: 37  DGHVERPPIIPNVACSTTASDKG----VTAKDVLIDIFTNTWARIYIPNSQRN------- 96
           DG + R   + N  C+    D        +KD+ ++   +TW R+Y+P+S  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 97  -LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYDDGFN 156
            LP++VY+HGGGF + S     +H+F   +A     +++S +YRL+PE+RLPAAYDDG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 157 ALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLGHNESTLLKPLTL 216
           AL W+K        + +W   + + S +FL G SAG N+AYN   R   + S  L PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSD-LSPLQI 200

Query: 217 KGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWSNPLV- 276
           +G+IL  PFFGGE R++SE           +    +D  W LSLP   +RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESEIRLMNDQVCPPI---VTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 277 NGSAKLKE---MKLPPLMVSISELDILLDRNLEFCAAMAGAGKKVERVILKGVGHGFQIL 336
           +GS KL++   ++   +M+   E D ++D   +    M   G +V      G  HG +I 
Sbjct: 261 DGSEKLEKIGRLRWKVMMIG-GEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIR 320

Query: 337 NKDQISTQRTQELMGSIRAFI 342
           +  +  T     L  SI+ FI
Sbjct: 321 DPSKRKT-----LFLSIKNFI 323