Carg20255 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20255
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionglutamic acid-rich protein-like
LocationCarg_Chr02: 4647564 .. 4648580 (-)
RNA-Seq ExpressionCarg20255
SyntenyCarg20255
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGACGATCACCGGAAATGTGGTTTCTTCGAAGCCAATCTCCCTTTCCAAAGCGGCGTCCACACTCTCCTCCTTTCTCTCCGTCGATAATGGCGCTTCGAAAGCTCTCTGTGCCTACCTGAGACGCGCCTCTGCCTCCTTCAACGAGTTAAAGCAGCTTCATAAGGACCTCAAGTCTTCGCGGTCCGATCGGAATCCCCGACATCACGGATTCGAGGTTTCAAGTGGTTTAGAGGCTGCTGTAGATAGTTCACATCGGATCGAAAACGGTGAGAGGATCAAATCTTCTGTAAATGAGAGCAAAAGCGGGAAGAAGCGGCGGGAGAGTAGGGATCGGAATGAGAACGAGCAGGATGGGAAAACAGCAATGGCAAGCGGTGGGAATGGTGATTTTGAGGATGTAGTGGGTGAAGATGGAAAGAGAAGGAGCGACGAATTGAAGACTGAAATTGAAGAAAAACCTAGCCGAAGAGTCGAGGTCGATGTGAAATCAAGCGATAGAGATGAGAGCGTTGTAGGAATTGAGAAGAAGAAAAAAAAGCACAAGAAAAAGAGCAAGGATGGAGAAGATGAACGTGATGCTGAAGTCGGGCAAAGTTACGGTAAATCGCAAACTAGTGCTAATAATGGCGAGACTGAAGCTACTGAAGATTTCATTGAGAACAATGTAGGAAGGGGAAAAGATAGAAAGAAGCATAAGGACAAGAATAATTTGGGGGATGAAAAGAGGACAAAGAAGGACAATGATGATGAAACAGGTCTTGTGGAGCTGTCGACTAAGGATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAACAGGGAAGAAGATGATGACGATCTTCAAAATAACAGTGGTGGGGCTATAGAGAAAGAGAAAATGCCAGTTTCGGATAGCCAAGAACTGAAAAGGAAAGAAAGTAAGAAGAGGAAAAATGGAGACTTGGAAGAAGGGGTAGATGATGGTTCAGAGGAGCAGCAGGGTACGAAGAGAAGGAAAAGAAAGTCATGA

mRNA sequence

ATGAAGACGATCACCGGAAATGTGGTTTCTTCGAAGCCAATCTCCCTTTCCAAAGCGGCGTCCACACTCTCCTCCTTTCTCTCCGTCGATAATGGCGCTTCGAAAGCTCTCTGTGCCTACCTGAGACGCGCCTCTGCCTCCTTCAACGAGTTAAAGCAGCTTCATAAGGACCTCAAGTCTTCGCGGTCCGATCGGAATCCCCGACATCACGGATTCGAGGTTTCAAGTGGTTTAGAGGCTGCTGTAGATAGTTCACATCGGATCGAAAACGGTGAGAGGATCAAATCTTCTGTAAATGAGAGCAAAAGCGGGAAGAAGCGGCGGGAGAGTAGGGATCGGAATGAGAACGAGCAGGATGGGAAAACAGCAATGGCAAGCGGTGGGAATGGTGATTTTGAGGATGTAGTGGGTGAAGATGGAAAGAGAAGGAGCGACGAATTGAAGACTGAAATTGAAGAAAAACCTAGCCGAAGAGTCGAGGTCGATGTGAAATCAAGCGATAGAGATGAGAGCGTTGTAGGAATTGAGAAGAAGAAAAAAAAGCACAAGAAAAAGAGCAAGGATGGAGAAGATGAACGTGATGCTGAAGTCGGGCAAAGTTACGGTAAATCGCAAACTAGTGCTAATAATGGCGAGACTGAAGCTACTGAAGATTTCATTGAGAACAATGTAGGAAGGGGAAAAGATAGAAAGAAGCATAAGGACAAGAATAATTTGGGGGATGAAAAGAGGACAAAGAAGGACAATGATGATGAAACAGGTCTTGTGGAGCTGTCGACTAAGGATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAACAGGGAAGAAGATGATGACGATCTTCAAAATAACAGTGGTGGGGCTATAGAGAAAGAGAAAATGCCAGTTTCGGATAGCCAAGAACTGAAAAGGAAAGAAAGTAAGAAGAGGAAAAATGGAGACTTGGAAGAAGGGGTAGATGATGGTTCAGAGGAGCAGCAGGGTACGAAGAGAAGGAAAAGAAAGTCATGA

Coding sequence (CDS)

ATGAAGACGATCACCGGAAATGTGGTTTCTTCGAAGCCAATCTCCCTTTCCAAAGCGGCGTCCACACTCTCCTCCTTTCTCTCCGTCGATAATGGCGCTTCGAAAGCTCTCTGTGCCTACCTGAGACGCGCCTCTGCCTCCTTCAACGAGTTAAAGCAGCTTCATAAGGACCTCAAGTCTTCGCGGTCCGATCGGAATCCCCGACATCACGGATTCGAGGTTTCAAGTGGTTTAGAGGCTGCTGTAGATAGTTCACATCGGATCGAAAACGGTGAGAGGATCAAATCTTCTGTAAATGAGAGCAAAAGCGGGAAGAAGCGGCGGGAGAGTAGGGATCGGAATGAGAACGAGCAGGATGGGAAAACAGCAATGGCAAGCGGTGGGAATGGTGATTTTGAGGATGTAGTGGGTGAAGATGGAAAGAGAAGGAGCGACGAATTGAAGACTGAAATTGAAGAAAAACCTAGCCGAAGAGTCGAGGTCGATGTGAAATCAAGCGATAGAGATGAGAGCGTTGTAGGAATTGAGAAGAAGAAAAAAAAGCACAAGAAAAAGAGCAAGGATGGAGAAGATGAACGTGATGCTGAAGTCGGGCAAAGTTACGGTAAATCGCAAACTAGTGCTAATAATGGCGAGACTGAAGCTACTGAAGATTTCATTGAGAACAATGTAGGAAGGGGAAAAGATAGAAAGAAGCATAAGGACAAGAATAATTTGGGGGATGAAAAGAGGACAAAGAAGGACAATGATGATGAAACAGGTCTTGTGGAGCTGTCGACTAAGGATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAACAGGGAAGAAGATGATGACGATCTTCAAAATAACAGTGGTGGGGCTATAGAGAAAGAGAAAATGCCAGTTTCGGATAGCCAAGAACTGAAAAGGAAAGAAAGTAAGAAGAGGAAAAATGGAGACTTGGAAGAAGGGGTAGATGATGGTTCAGAGGAGCAGCAGGGTACGAAGAGAAGGAAAAGAAAGTCATGA

Protein sequence

MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKSSRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDGKTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKKKHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLGDEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSDSQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Homology
BLAST of Carg20255 vs. NCBI nr
Match: KAG7035443.1 (hypothetical protein SDJN02_02239, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.8 bits (1527), Expect = 1.9e-165
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS
Sbjct: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG
Sbjct: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK
Sbjct: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180

Query: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240
           KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG
Sbjct: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240

Query: 241 DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300
           DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD
Sbjct: 241 DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300

Query: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 339
           SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Sbjct: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 338

BLAST of Carg20255 vs. NCBI nr
Match: KAG6605505.1 (hypothetical protein SDJN03_02822, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 585.5 bits (1508), Expect = 3.0e-163
Identity = 336/338 (99.41%), Postives = 336/338 (99.41%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS
Sbjct: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG
Sbjct: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK
Sbjct: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180

Query: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240
           KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG
Sbjct: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240

Query: 241 DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300
           DE RTKKDNDDETGLVELSTKD KKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD
Sbjct: 241 DENRTKKDNDDETGLVELSTKD-KKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300

Query: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 339
           SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Sbjct: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 337

BLAST of Carg20255 vs. NCBI nr
Match: XP_022947113.1 (DEAD-box ATP-dependent RNA helicase 42-like isoform X1 [Cucurbita moschata] >XP_022947114.1 DEAD-box ATP-dependent RNA helicase 42-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 580.9 bits (1496), Expect = 7.3e-162
Identity = 333/338 (98.52%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS
Sbjct: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG
Sbjct: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK
Sbjct: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180

Query: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240
           KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG
Sbjct: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240

Query: 241 DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300
           DEKRTKKDNDDETGLVELSTKD   KKKKKKKKKNREEDDDDLQNN GGAIEKEKMPVSD
Sbjct: 241 DEKRTKKDNDDETGLVELSTKD---KKKKKKKKKNREEDDDDLQNNGGGAIEKEKMPVSD 300

Query: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 339
            QELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Sbjct: 301 CQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 335

BLAST of Carg20255 vs. NCBI nr
Match: XP_023532260.1 (protein pxr-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.9 bits (1470), Expect = 7.5e-159
Identity = 329/338 (97.34%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRAS SFNELKQLHKDLKS
Sbjct: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASTSFNELKQLHKDLKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSV+ES+SGKKRRESRDRNENEQDG
Sbjct: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVSESRSGKKRRESRDRNENEQDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           KTAMASGGNGDFEDVVGEDGKRRSDEL+TEIEEKPSRRVEVDVKSSDRDESVVGIE KKK
Sbjct: 121 KTAMASGGNGDFEDVVGEDGKRRSDELRTEIEEKPSRRVEVDVKSSDRDESVVGIE-KKK 180

Query: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240
           KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEA+EDFIENNVGRGKDRKKHKDKNNLG
Sbjct: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEASEDFIENNVGRGKDRKKHKDKNNLG 240

Query: 241 DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300
           DEKRTKKDNDDETGLVELSTKD   KKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD
Sbjct: 241 DEKRTKKDNDDETGLVELSTKD---KKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300

Query: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 339
           SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Sbjct: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 334

BLAST of Carg20255 vs. NCBI nr
Match: XP_022947115.1 (sarcoplasmic reticulum histidine-rich calcium-binding protein-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 548.9 bits (1413), Expect = 3.1e-152
Identity = 320/338 (94.67%), Postives = 320/338 (94.67%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS
Sbjct: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG
Sbjct: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK
Sbjct: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180

Query: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240
           KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG
Sbjct: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240

Query: 241 DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300
           DEKRTKKDNDDETGLVELSTKD   KKKKKKKKKNREEDDDDLQNN GGAIE        
Sbjct: 241 DEKRTKKDNDDETGLVELSTKD---KKKKKKKKKNREEDDDDLQNNGGGAIE-------- 300

Query: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 339
                 KESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Sbjct: 301 ------KESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 321

BLAST of Carg20255 vs. ExPASy TrEMBL
Match: A0A6J1G5Q5 (DEAD-box ATP-dependent RNA helicase 42-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451081 PE=4 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.5e-162
Identity = 333/338 (98.52%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS
Sbjct: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG
Sbjct: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK
Sbjct: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180

Query: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240
           KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG
Sbjct: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240

Query: 241 DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300
           DEKRTKKDNDDETGLVELSTKD   KKKKKKKKKNREEDDDDLQNN GGAIEKEKMPVSD
Sbjct: 241 DEKRTKKDNDDETGLVELSTKD---KKKKKKKKKNREEDDDDLQNNGGGAIEKEKMPVSD 300

Query: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 339
            QELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Sbjct: 301 CQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 335

BLAST of Carg20255 vs. ExPASy TrEMBL
Match: A0A6J1G5Y0 (sarcoplasmic reticulum histidine-rich calcium-binding protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451081 PE=4 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.5e-152
Identity = 320/338 (94.67%), Postives = 320/338 (94.67%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS
Sbjct: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG
Sbjct: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK
Sbjct: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180

Query: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240
           KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG
Sbjct: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKDKNNLG 240

Query: 241 DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSD 300
           DEKRTKKDNDDETGLVELSTKD   KKKKKKKKKNREEDDDDLQNN GGAIE        
Sbjct: 241 DEKRTKKDNDDETGLVELSTKD---KKKKKKKKKNREEDDDDLQNNGGGAIE-------- 300

Query: 301 SQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 339
                 KESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Sbjct: 301 ------KESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 321

BLAST of Carg20255 vs. ExPASy TrEMBL
Match: A0A6J1KYC0 (nipped-B-like protein B OS=Cucurbita maxima OX=3661 GN=LOC111499847 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 8.5e-132
Identity = 298/342 (87.13%), Postives = 307/342 (89.77%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS
Sbjct: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDRNPR+HGFEVSSGLEAAVDSSHRIENGERIKSSV+ESKSGKKRRESRD NE+EQDG
Sbjct: 61  SRSDRNPRYHGFEVSSGLEAAVDSSHRIENGERIKSSVSESKSGKKRRESRDLNEDEQDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           KTAMASGGN DFE+VVGEDGKR+SDELK+EIE KPSRRVEVD+KSSDRDESVV IE KKK
Sbjct: 121 KTAMASGGNVDFENVVGEDGKRKSDELKSEIEGKPSRRVEVDLKSSDRDESVVAIE-KKK 180

Query: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKK-HKDKNNL 240
           KHKKKSKDGEDERDAEVGQSYGKSQTSANNGE   TEDF+ENNV R KDRKK HKDKN L
Sbjct: 181 KHKKKSKDGEDERDAEVGQSYGKSQTSANNGE---TEDFVENNVAREKDRKKRHKDKNTL 240

Query: 241 G---DEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKM 300
           G   DEKRTKKD DDETGLVELSTKD  KKKKKKKKKKNREED D LQNNSG AIE    
Sbjct: 241 GDEKDEKRTKKDIDDETGLVELSTKD--KKKKKKKKKKNREEDADVLQNNSGEAIE---- 300

Query: 301 PVSDSQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 339
                     KE+KKRKNGDLEEGVDDGSEEQQGTKRRKRKS
Sbjct: 301 ----------KENKKRKNGDLEEGVDDGSEEQQGTKRRKRKS 322

BLAST of Carg20255 vs. ExPASy TrEMBL
Match: A0A6J1JCJ7 (cylicin-1-like OS=Cucurbita maxima OX=3661 GN=LOC111484496 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 5.2e-89
Identity = 221/357 (61.90%), Postives = 260/357 (72.83%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKT+TG++VSSKPIS+SKAASTLSSFLSVDNGASKA+CAYLRRASASFNELKQLHK+LKS
Sbjct: 1   MKTVTGSIVSSKPISISKAASTLSSFLSVDNGASKAICAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNENEQDG 120
           SRSDR  RHHG E S+  EAA D+   IE+GE+       +K GK  + S   N   +DG
Sbjct: 61  SRSDRKHRHHGSEASNDPEAARDNPQWIEDGEKKNPLYLRAKDGKSGKTS--LNVQSEDG 120

Query: 121 KTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESVVGIEKKKK 180
           K    SGGNGDFED  GE  KR+ ++LKTEIE+KP+R+VE+DV+SSD+D+SVV +EKK K
Sbjct: 121 KAEKESGGNGDFEDASGEYRKRKVEDLKTEIEDKPNRKVEMDVESSDKDKSVVAVEKKGK 180

Query: 181 KHKKKSKD-----GEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKKHKD 240
           KH+KKS+D      +DE  A   +S  KS+ S NNGE EA+  F+ENN+  GKDRKKH D
Sbjct: 181 KHQKKSEDRYAKIEDDEHKAGARRSSSKSRNSDNNGEIEASAKFVENNIASGKDRKKHVD 240

Query: 241 KNNLGD-----------------EKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREE 300
           K +LGD                 EK T KDNDD        T+  KKKKKKKKKKKNREE
Sbjct: 241 KKSLGDDKDQVKSEGHRRRDAEEEKSTNKDNDD-------GTESSKKKKKKKKKKKNREE 300

Query: 301 DDDDLQNNSGGAIEKEKMPVSDSQELKRKESKKRKNGDLEEGVDDGSEEQQGTKRRK 336
           +DDD QNNSGGA+ KE++PV D +ELKRKE KKRKN DLEEG DDGSEEQQ TKRRK
Sbjct: 301 EDDDFQNNSGGALVKEEIPVLDDKELKRKEKKKRKNRDLEEGGDDGSEEQQRTKRRK 348

BLAST of Carg20255 vs. ExPASy TrEMBL
Match: A0A6J1CXN0 (nuclear speckle splicing regulatory protein 1 OS=Momordica charantia OX=3673 GN=LOC111015519 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.5e-88
Identity = 231/384 (60.16%), Postives = 261/384 (67.97%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKT+TG V+SSKPISLSKAASTLSSFLS DNGAS A CAYLRRASASFNELKQLHK+LKS
Sbjct: 1   MKTVTGRVISSKPISLSKAASTLSSFLSTDNGASPAFCAYLRRASASFNELKQLHKELKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDS----------SHRIENGERIKSSVNESKSGKKRRES 120
           SRSDR  RHH  EV SGLE AVD           +HRIE GE+  SSV+ESKSGKKRR+S
Sbjct: 61  SRSDRKHRHHRSEVFSGLEVAVDETYRSEASHGINHRIEGGEKKNSSVSESKSGKKRRDS 120

Query: 121 RDRNENE----------------QDGKTAMASGGNGDFEDVVGEDGKRRSDELKTEIEEK 180
           RDR E++                +D +      GNG FE V+GEDGKRRSDELKTE+E K
Sbjct: 121 RDRTEDKPALSVQSDRESGREYREDARADGKRNGNGGFEGVIGEDGKRRSDELKTEVERK 180

Query: 181 PSRRVEVDVKSSDRDESVVGIEKKKKKHKKKS-----KDGEDERDAEVGQSYGKSQTSAN 240
           PSRRVE+DV+SSD  +S V +E ++KKHKKKS     K G+DERDA   QSY KS+ S N
Sbjct: 181 PSRRVEMDVESSDGVKSSVAVESRRKKHKKKSEEEHGKTGDDERDAGARQSYSKSRISDN 240

Query: 241 NGETEATE-DFIENNVGRGKDRKKHKDKNNLGDEK-----------------RTKKDNDD 300
           NGE EA   D +ENNV RGKDRK  KDK N+GDE+                    K+N D
Sbjct: 241 NGEIEAAAGDLVENNVARGKDRK--KDKKNVGDERDKVRSEGRRRRDVEAEETADKNNGD 300

Query: 301 ETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIEKEKMPVSDSQELKRKESKK 336
              LVEL T         KK KK REED  DLQNNSGGA+E++ MPV DS++LKRKE KK
Sbjct: 301 WKDLVELPT---------KKNKKKREEDAGDLQNNSGGAVERKGMPVLDSKKLKRKEKKK 360

BLAST of Carg20255 vs. TAIR 10
Match: AT5G60030.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75335.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 75.5 bits (184), Expect = 9.3e-14
Identity = 113/344 (32.85%), Postives = 169/344 (49.13%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKT+TG VVS++PISLSKAA  LS F S DNGAS+ + AYLRRASA+F ELK  H+++KS
Sbjct: 1   MKTVTGRVVSAEPISLSKAAKLLSGFASSDNGASQDVSAYLRRASAAFTELKSFHREIKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRESRDRNE----N 120
                                          E   SS  E+KS + ++ S  ++E    +
Sbjct: 61  K------------------------------ETKPSSDRETKSTETKQSSDAKSERNVID 120

Query: 121 EQDGKTAMASGGNG-DFEDVVG---EDGKRRSDELKTEIEEKPSRRVEVDVKSSDRDESV 180
           E DG+            E V G   ++ K +  +    ++EK + ++E + +S +R    
Sbjct: 121 EFDGRKIRYRNSEAVSVESVYGRERDEKKMKKSKDADVVDEKVNEKLEAEQRSEERR--- 180

Query: 181 VGIEKKKKKHKKKSKDGEDERDAEVGQSYGKSQTSANNGETEATEDFIENNVGRGKDRKK 240
              E+KK+K KKK+   ED  D +V +     Q SA                    DRK+
Sbjct: 181 ---ERKKEKKKKKNNKDEDVVDEKVKEKLEDEQKSA--------------------DRKE 240

Query: 241 HKDKNNLGDEKRTKKDNDDETGLVELSTKDKKKKKKKKKKKKNREEDDDDLQNNSGGAIE 300
            K       +K++KK+ND++    +   +D++K  + K+KKKN++ED  D         E
Sbjct: 241 RK-------KKKSKKNNDEDVVDEKEKLEDEQKSAEIKEKKKNKDEDVVD---------E 269

Query: 301 KEKMPVSDSQEL-KRKESKKRKNGDLEEGVDDGSEEQQGTKRRK 336
           KEK  + D Q   +RK+ KK+K    EE V   SEE++  K+RK
Sbjct: 301 KEKEKLEDEQRSGERKKEKKKKRKSDEEIV---SEERKSKKKRK 269

BLAST of Carg20255 vs. TAIR 10
Match: AT1G75335.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G60030.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 67.4 bits (163), Expect = 2.5e-11
Identity = 48/110 (43.64%), Postives = 63/110 (57.27%), Query Frame = 0

Query: 1   MKTITGNVVSSKPISLSKAASTLSSFLSVDNGASKALCAYLRRASASFNELKQLHKDLKS 60
           MKT+TG V S+KPISLSKAA+ LS F+S +NGAS+ + AYLRRAS +F ELK +H+++KS
Sbjct: 1   MKTVTGRVNSAKPISLSKAATLLSGFVSSENGASQDVSAYLRRASGAFIELKSIHREIKS 60

Query: 61  SRSDRNPRHHGFEVSSGLEAAVDSSHRIENGERIKSSVNESKSGKKRRES 111
             +                    SS +     R   S    KS KKR+ S
Sbjct: 61  KETKL------------------SSKKKRKSHREMGSEERKKSKKKRKSS 92

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035443.11.9e-165100.00hypothetical protein SDJN02_02239, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6605505.13.0e-16399.41hypothetical protein SDJN03_02822, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022947113.17.3e-16298.52DEAD-box ATP-dependent RNA helicase 42-like isoform X1 [Cucurbita moschata] >XP_... [more]
XP_023532260.17.5e-15997.34protein pxr-1-like [Cucurbita pepo subsp. pepo][more]
XP_022947115.13.1e-15294.67sarcoplasmic reticulum histidine-rich calcium-binding protein-like isoform X2 [C... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1G5Q53.5e-16298.52DEAD-box ATP-dependent RNA helicase 42-like isoform X1 OS=Cucurbita moschata OX=... [more]
A0A6J1G5Y01.5e-15294.67sarcoplasmic reticulum histidine-rich calcium-binding protein-like isoform X2 OS... [more]
A0A6J1KYC08.5e-13287.13nipped-B-like protein B OS=Cucurbita maxima OX=3661 GN=LOC111499847 PE=4 SV=1[more]
A0A6J1JCJ75.2e-8961.90cylicin-1-like OS=Cucurbita maxima OX=3661 GN=LOC111484496 PE=4 SV=1[more]
A0A6J1CXN01.5e-8860.16nuclear speckle splicing regulatory protein 1 OS=Momordica charantia OX=3673 GN=... [more]
Match NameE-valueIdentityDescription
AT5G60030.19.3e-1432.85unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G75335.12.5e-1143.64unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 261..286
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 219..261
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 274..288
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..332
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..119
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..72
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..338
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..217
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..176
NoneNo IPR availablePANTHERPTHR48227DNA TOPOISOMERASE 1-LIKEcoord: 1..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20255-RACarg20255-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004386 helicase activity