Carg20233 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20233
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein disulfide-isomerase 5-1
Location	Carg_Chr02: 4778315 .. 4780905 (+)
RNA-Seq Expression	Carg20233
Synteny	Carg20233

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATTCATAAATTAAATTTGATAAAATAAATTAAAATTTCAAGGTATTCAACATCGGGCCCCAACGACGCTTTCCAGATCGACCGGGATCGTTGTTTCAATTGCATATTGCGAACTCCGGCCACCAATCCTGATCTTTTCTTCCATTGAATCCACAGCACTAGATAAGATCCAGTGACCACATTCAACCATGAAATTCCATATTTCATCTGCAATTTTGTCTTTCTTGTTAATTTCTTTTATCGTTCTAAACACAGCTTCTCTCTGCAAATCTGAAGTTATAACCCTAACTGCGGACACCTTCTCCGACAAGGTACCGTTTTCAAACCCTAATTTACTCACTGAGGTCGATTTCTGTATTCTCTAAGTGGAAAATTTTGTCTGCGAGGGAATGATTCTCTTGATTTTTGAATCTTGTTTGACGATTCAGTATGTTTTTTTTTTTAATTTTAATTGATGTTCCTATAAAATGGATGATGCATGGTTGAATCCAGTTGTTTTTGTATTCGAGTTACTCTATGAGTTCGTTGGATTGGAGCGATTCGTCTGGGATTGGTATGGCTACTGTCAGCCGGGATGTCGCAGTTTGGGATCCGCAAGAACGAACTGCGGCCGTTCGAATAAAAAAGAAGAAGAAAATTTGATAAGATTGTCTAGAATTGTCGAACTTTATTCTGAATATTATTGTGAACATCCAATGACAAATGCAGTAGAAACTAACTTCTGGTCTTGGTTTTTGATCGGATTTCGGGCAAACTGGGGCCAACAGTTGAGACCAAATTACTTGCTCGTGGGGATTGAACAAATTTGATTAGTGTAGGTTGTTAAGCAGGACTATGATTGGACAGATTAAGTTTGTTGTTCCTAGATTTAGTGACATTGGTGAAACTTGTTAGGAAGGCTCATAAATGGTTAATAGTAAAGTGCATAGAGGGTATGCGTTCATGTCATGACGACCGCCTAATTAGGATTTAATGTTCTCTGAATTACCTTTGCAATCAAATCCCGGTGGATAAAATAGTTGTCTCTTGAGAATAATCGAGTTGACTTGGAAACTATGGATGTCATGTCATTTTGAGTTTGCAGGCCGATGGAACTCTCACCTAGTTATTGATACGGTGTTGTCCATTATAACCTTTCATTGAAGATTGGTTAAATCAGCTTTCATCCTAGGCTTAAAGCTTTGATCTTTGGCCTAGATGATGCATGGTTGGTTATCTTCCCTTCAGTTTATATGTTATATCTTGATGGATTTTCATTTTTCTTCTTATATCATCCAACATTGATTAATCAAGTATTCAAAATAACAGAACCAACATTTGAAATTGTGGATTCCGACTAAGATATGATTTCACCGAACTTTTTAGTACATTCCTCATCGTAGAAATATGTACTTTTCAGGTAAAGGAAAAAGATACCGCATGGTTTGTGAAGTTCTGTGTTCCTTGGTGCAAGCATTGGTTCGCATTCTTTCTTATCGTAGTTTCCTGGTTTTTATCCTGTCGGAAATCGAGTTCATATTGCTTAATTACCTGTAACTTAAATTGCAAATTTCAGCAAGAATTTAGGATCATTATGGGAGGACCTGGGCAAGACAATGGAAGGCGAGGATGAAATCGAGGTCGGAGAAGTTGATTGTGGTTCTCACAAATCAGTCTGTTCTAAAGTTGATATCCATTCATATCCTACATTCAAGCTTTTCTATGAAGGGGAAGAAGTTGCAAAGTATAAAGGTGAACTTTTCCTCCCCTTTTTGTTACCCTTATTGTCTATGTTCTATATGTGTTCCGATTTTGCTTCTACTTTGGCAATTTATTTAATGAAGCTTTGTCGTCTTTGCGCATTCTTCACACGTAAGCACACATCAATTAAAAAAGATAAACTCATTGGTCTAATGAATCATGTTGAGCTTAGTTCAAAGTCTTATTAAACGTTGCCAATTTCCATCCTTCAAAATTTGATGATTAGCAAGTTACGATATATTTGTTCATATCGACATGTCAGTTCATTCACCTCGTGGTTCTGCTATAGATGATTTTTGTTTACGAATATCAGAAATAAGAATGAAATGCCTCATTTCATTATATTCTTGGATCCGGATGAACCCTGATATAGCAGCTATAATTTTATGATGACCCAGGGCCAAGAGACGTAGAATCTCTCAAAAGCTTTGTTCTTGAAGAAGCTGAGAAAGCAGTGGAAAAAGCACAGCAGGACAGTGCTAAAGAATTGTGAAGAAAAAGATAAAGAACAGCGATTATATTGGTAGGGAAAACTTTTAACTTATTCACAGGAGTTCTTTCTTACCCATTCCCTCGAAGATTTGATAAACATATTTGATTTTGACAATCAGATAGATTCCTTTCCATGCTGTGTTAGACTATAAACGCTTGAAGCTTCTTGCAATGTTCTTAGAAGAATGCTTAAATTTGGATGGATAGATTTTGACCGTAATCTCAGTGACATTGTTTTCTGCCTCTTATGTTTAGGCTACTGCCTTTTGGTTGGAATATGTGAAAGATATTAAAGAATTTTGTACCGATAGGTTTATTATATATTTTATACCTTGCATTATATATAGACCGTTAAT

mRNA sequence

AAAAATTCATAAATTAAATTTGATAAAATAAATTAAAATTTCAAGGTATTCAACATCGGGCCCCAACGACGCTTTCCAGATCGACCGGGATCGTTGTTTCAATTGCATATTGCGAACTCCGGCCACCAATCCTGATCTTTTCTTCCATTGAATCCACAGCACTAGATAAGATCCAGTGACCACATTCAACCATGAAATTCCATATTTCATCTGCAATTTTGTCTTTCTTGTTAATTTCTTTTATCGTTCTAAACACAGCTTCTCTCTGCAAATCTGAAGTTATAACCCTAACTGCGGACACCTTCTCCGACAAGGTAAAGGAAAAAGATACCGCATGGTTTGTGAAGTTCTGTGTTCCTTGGTGCAAGCATTGGTTCGCATTCTTTCTTATCAATTTAGGATCATTATGGGAGGACCTGGGCAAGACAATGGAAGGCGAGGATGAAATCGAGGTCGGAGAAGTTGATTGTGGTTCTCACAAATCAGTCTGTTCTAAAGTTGATATCCATTCATATCCTACATTCAAGCTTTTCTATGAAGGGGAAGAAGTTGCAAAGTATAAAGGGCCAAGAGACGTAGAATCTCTCAAAAGCTTTGTTCTTGAAGAAGCTGAGAAAGCAGTGGAAAAAGCACAGCAGGACAGTGCTAAAGAATTGTGAAGAAAAAGATAAAGAACAGCGATTATATTGGTAGGGAAAACTTTTAACTTATTCACAGGAGTTCTTTCTTACCCATTCCCTCGAAGATTTGATAAACATATTTGATTTTGACAATCAGATAGATTCCTTTCCATGCTGTGTTAGACTATAAACGCTTGAAGCTTCTTGCAATGTTCTTAGAAGAATGCTTAAATTTGGATGGATAGATTTTGACCGTAATCTCAGTGACATTGTTTTCTGCCTCTTATGTTTAGGCTACTGCCTTTTGGTTGGAATATGTGAAAGATATTAAAGAATTTTGTACCGATAGGTTTATTATATATTTTATACCTTGCATTATATATAGACCGTTAAT

Coding sequence (CDS)

ATGAAATTCCATATTTCATCTGCAATTTTGTCTTTCTTGTTAATTTCTTTTATCGTTCTAAACACAGCTTCTCTCTGCAAATCTGAAGTTATAACCCTAACTGCGGACACCTTCTCCGACAAGGTAAAGGAAAAAGATACCGCATGGTTTGTGAAGTTCTGTGTTCCTTGGTGCAAGCATTGGTTCGCATTCTTTCTTATCAATTTAGGATCATTATGGGAGGACCTGGGCAAGACAATGGAAGGCGAGGATGAAATCGAGGTCGGAGAAGTTGATTGTGGTTCTCACAAATCAGTCTGTTCTAAAGTTGATATCCATTCATATCCTACATTCAAGCTTTTCTATGAAGGGGAAGAAGTTGCAAAGTATAAAGGGCCAAGAGACGTAGAATCTCTCAAAAGCTTTGTTCTTGAAGAAGCTGAGAAAGCAGTGGAAAAAGCACAGCAGGACAGTGCTAAAGAATTGTGA

Protein sequence

MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL

Homology

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BLAST of Carg20233 vs. NCBI nr
Match: KAG7035463.1 (Protein disulfide-isomerase 5-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 309.7 bits (792), Expect = 1.4e-80
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH
Sbjct: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
           WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL
Sbjct: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 155

[Close]

BLAST of Carg20233 vs. NCBI nr
Match: KAG6605525.1 (Protein disulfide-isomerase 5-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 287.0 bits (733), Expect = 1.0e-73
Identity = 148/155 (95.48%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH
Sbjct: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL
Sbjct: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 150

[Close]

BLAST of Carg20233 vs. NCBI nr
Match: XP_022947942.1 (protein disulfide-isomerase 5-1 [Cucurbita moschata])

HSP 1 Score: 284.6 bits (727), Expect = 4.9e-73
Identity = 147/155 (94.84%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           MKFHISSAILSFLLIS IVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH
Sbjct: 1   MKFHISSAILSFLLISLIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL
Sbjct: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 150

[Close]

BLAST of Carg20233 vs. NCBI nr
Match: XP_023007347.1 (protein disulfide-isomerase 5-1 [Cucurbita maxima])

HSP 1 Score: 282.7 bits (722), Expect = 1.9e-72
Identity = 145/155 (93.55%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           MKFHISSAILSFLLIS I+LNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH
Sbjct: 1   MKFHISSAILSFLLISLILLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWED+GKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDMGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL
Sbjct: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 150

[Close]

BLAST of Carg20233 vs. NCBI nr
Match: XP_023532319.1 (protein disulfide-isomerase 5-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 282.3 bits (721), Expect = 2.5e-72
Identity = 146/155 (94.19%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           MKFHISSAILSFLLI  IVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH
Sbjct: 1   MKFHISSAILSFLLIFLIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL
Sbjct: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 150

[Close]

BLAST of Carg20233 vs. ExPASy Swiss-Prot
Match: Q8GYD1 (Protein disulfide-isomerase 5-1 OS=Arabidopsis thaliana OX=3702 GN=PDIL5-1 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.3e-49
Identity = 98/142 (69.01%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 14  LISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLINLGSLW 73
           +I  ++     L K+EVITLT +TFSDK+KEKDTAWFVKFCVPWCKH        LG+LW
Sbjct: 11  MIILLLFIPIELVKAEVITLTPETFSDKIKEKDTAWFVKFCVPWCKH-----CKKLGNLW 70

Query: 74  EDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPRDVESLK 133
           EDLGK MEG+DEIEVGEVDCG+ ++VC+KV+IHSYPTF LFY GEEV+KYKG RDVESLK
Sbjct: 71  EDLGKAMEGDDEIEVGEVDCGTSRAVCTKVEIHSYPTFMLFYNGEEVSKYKGKRDVESLK 130

Query: 134 SFVLEEAEKAVEKAQQDSAKEL 156
           +FV+EE EKA EKAQ +  KEL
Sbjct: 131 AFVVEETEKAAEKAQLED-KEL 146

[Close]

BLAST of Carg20233 vs. ExPASy Swiss-Prot
Match: Q10N04 (Protein disulfide isomerase-like 5-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDIL5-1 PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 8.8e-49
Identity = 99/144 (68.75%), Postives = 113/144 (78.47%), Query Frame = 0


Query: 8   AILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLI 67
           A L   L   +++  A+   +EVITLT +TFSDK+KEKDT WFVKFCVPWCKH       
Sbjct: 9   ARLLVALALVVLVALAARSGAEVITLTEETFSDKIKEKDTVWFVKFCVPWCKH-----CK 68

Query: 68  NLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPR 127
           NLG+LWEDLGK MEG DEIE+G+VDCG  K VCSKVDIHSYPTFK+FYEGEEVAKYKGPR
Sbjct: 69  NLGTLWEDLGKVMEGADEIEIGQVDCGVSKPVCSKVDIHSYPTFKVFYEGEEVAKYKGPR 128

Query: 128 DVESLKSFVLEEAEKAVEKAQQDS 152
           +VESLK+FV +EAEKA E   QDS
Sbjct: 129 NVESLKNFVSDEAEKAGEAKLQDS 147

[Close]

BLAST of Carg20233 vs. ExPASy Swiss-Prot
Match: Q91W90 (Thioredoxin domain-containing protein 5 OS=Mus musculus OX=10090 GN=Txndc5 PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.1e-14
Identity = 46/129 (35.66%), Postives = 71/129 (55.04%), Query Frame = 0


Query: 27  KSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLINLGSLWEDLGKTMEGEDEI 86
           K  +  L+A+ F   V + +   F+KF  PWC H  A     L   WE L   +E  + +
Sbjct: 174 KQGLYELSANNFELHVSQGN--HFIKFFAPWCGHCKA-----LAPTWEQLALGLEHSETV 233

Query: 87  EVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPRDVESLKSFV---LEEAEKA 146
           ++G+VDC  H +VCS+  +  YPT   F +G++V +YKG RD+ESL+ +V   L+ +E A
Sbjct: 234 KIGKVDCTQHYAVCSEHQVRGYPTLLWFRDGKKVDQYKGKRDLESLRDYVQSQLQGSEAA 293

Query: 147 VEKAQQDSA 153
            E  +   A
Sbjct: 294 PETVEPSEA 295

[Close]

BLAST of Carg20233 vs. ExPASy Swiss-Prot
Match: Q9VYV3 (Thioredoxin domain-containing protein 5 homolog OS=Drosophila melanogaster OX=7227 GN=prtp PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 2.4e-14
Identity = 41/126 (32.54%), Postives = 66/126 (52.38%), Query Frame = 0


Query: 29  EVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLINLGSLWEDLGKTMEGEDEIEV 88
           +V+ LT DTF+  V   +   FVKF  PWC H        L   WEDL K +  E  + +
Sbjct: 167 KVVDLTEDTFAKHVSTGN--HFVKFFAPWCSH-----CQRLAPTWEDLAKELIKEPTVTI 226

Query: 89  GEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPRDVESLKSFVLEEAEKAVEKAQ 148
            ++DC   +S+C   ++  YPT     +G+++ KY G RD+ +LK++V +     +EK  
Sbjct: 227 SKIDCTQFRSICQDFEVKGYPTLLWIEDGKKIEKYSGARDLSTLKTYVEKMVGVPLEKTA 285

Query: 149 QDSAKE 155
            ++  E
Sbjct: 287 GEAGDE 285

[Close]

BLAST of Carg20233 vs. ExPASy Swiss-Prot
Match: Q8NBS9 (Thioredoxin domain-containing protein 5 OS=Homo sapiens OX=9606 GN=TXNDC5 PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 3.2e-14
Identity = 41/116 (35.34%), Postives = 63/116 (54.31%), Query Frame = 0


Query: 27  KSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLINLGSLWEDLGKTMEGEDEI 86
           K  +  L+A  F   V + D   F+KF  PWC H  A     L   WE L   +E  + +
Sbjct: 188 KQGLYELSASNFELHVAQGD--HFIKFFAPWCGHCKA-----LAPTWEQLALGLEHSETV 247

Query: 87  EVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPRDVESLKSFVLEEAEK 143
           ++G+VDC  H  +CS   +  YPT   F +G++V +YKG RD+ESL+ +V  + ++
Sbjct: 248 KIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQR 296

[Close]

BLAST of Carg20233 vs. ExPASy TrEMBL
Match: A0A6J1G7T1 (protein disulfide-isomerase 5-1 OS=Cucurbita moschata OX=3662 GN=LOC111451672 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.4e-73
Identity = 147/155 (94.84%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           MKFHISSAILSFLLIS IVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH
Sbjct: 1   MKFHISSAILSFLLISLIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL
Sbjct: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 150

[Close]

BLAST of Carg20233 vs. ExPASy TrEMBL
Match: A0A6J1KYF6 (protein disulfide-isomerase 5-1 OS=Cucurbita maxima OX=3661 GN=LOC111499869 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 9.1e-73
Identity = 145/155 (93.55%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           MKFHISSAILSFLLIS I+LNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH
Sbjct: 1   MKFHISSAILSFLLISLILLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWED+GKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDMGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL
Sbjct: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 150

[Close]

BLAST of Carg20233 vs. ExPASy TrEMBL
Match: A0A0A0KFX1 (Thioredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290880 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.9e-63
Identity = 126/155 (81.29%), Postives = 137/155 (88.39%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           M+FHISSAI  FL IS ++LNTASL ++EV+TLTADTFSDK+KEKDTAWFVKFCVPWCKH
Sbjct: 1   MRFHISSAIFPFLFISLLLLNTASLYRAEVLTLTADTFSDKIKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWEDLGK MEGEDEIEVGEVDCGS+K VCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDLGKAMEGEDEIEVGEVDCGSNKPVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESL+ F LEE+EKA EKA+QDS KEL
Sbjct: 121 AKYKGPRDVESLRIFALEESEKAAEKAEQDSDKEL 150

[Close]

BLAST of Carg20233 vs. ExPASy TrEMBL
Match: A0A6J1CGL4 (protein disulfide isomerase-like 5-1 OS=Momordica charantia OX=3673 GN=LOC111011347 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.8e-63
Identity = 129/155 (83.23%), Postives = 134/155 (86.45%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           M+FHI SAI   LLIS +VLNTASLC++EVITLTADTFSDKVKEKDTAWFVKFCVPWCKH
Sbjct: 1   MRFHILSAIFPLLLISVLVLNTASLCRAEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWEDLGK MEGEDEIEVGEVDC  +K VCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDLGKAMEGEDEIEVGEVDCSLNKPVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKY GPRDVESLKSFVLEEAEKA EK QQDS  EL
Sbjct: 121 AKYSGPRDVESLKSFVLEEAEKAEEKVQQDSDNEL 150

[Close]

BLAST of Carg20233 vs. ExPASy TrEMBL
Match: A0A1S3CKB7 (protein disulfide-isomerase 5-1 OS=Cucumis melo OX=3656 GN=LOC103501930 PE=4 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.7e-61
Identity = 122/155 (78.71%), Postives = 134/155 (86.45%), Query Frame = 0


Query: 1   MKFHISSAILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKH 60
           M+FHISS I  FL IS ++LNTASL ++EV+TLTADTFSDK+KEKDT WFVKFCVPWCKH
Sbjct: 1   MRFHISSPIFPFLFISLLLLNTASLHRAEVLTLTADTFSDKIKEKDTVWFVKFCVPWCKH 60

Query: 61  WFAFFLINLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEV 120
                  NLGSLWEDLGK MEGEDEIEVGEVDCGS+K VCSKVDIHSYPTFKLFYEGEEV
Sbjct: 61  -----CKNLGSLWEDLGKAMEGEDEIEVGEVDCGSNKPVCSKVDIHSYPTFKLFYEGEEV 120

Query: 121 AKYKGPRDVESLKSFVLEEAEKAVEKAQQDSAKEL 156
           AKYKGPRDVESL+ F LEE+EKA  KA++DS KEL
Sbjct: 121 AKYKGPRDVESLRIFALEESEKAAAKAEEDSDKEL 150

[Close]

BLAST of Carg20233 vs. TAIR 10
Match: AT1G07960.2 (PDI-like 5-1 )

HSP 1 Score: 196.4 bits (498), Expect = 1.7e-50
Identity = 98/142 (69.01%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 14  LISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLINLGSLW 73
           +I  ++     L K+EVITLT +TFSDK+KEKDTAWFVKFCVPWCKH        LG+LW
Sbjct: 11  MIILLLFIPIELVKAEVITLTPETFSDKIKEKDTAWFVKFCVPWCKH-----CKKLGNLW 70

Query: 74  EDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPRDVESLK 133
           EDLGK MEG+DEIEVGEVDCG+ ++VC+KV+IHSYPTF LFY GEEV+KYKG RDVESLK
Sbjct: 71  EDLGKAMEGDDEIEVGEVDCGTSRAVCTKVEIHSYPTFMLFYNGEEVSKYKGKRDVESLK 130

Query: 134 SFVLEEAEKAVEKAQQDSAKEL 156
           +FV+EE EKA EKAQ +  KEL
Sbjct: 131 AFVVEETEKAAEKAQLED-KEL 146

[Close]

BLAST of Carg20233 vs. TAIR 10
Match: AT1G07960.3 (PDI-like 5-1 )

HSP 1 Score: 196.4 bits (498), Expect = 1.7e-50
Identity = 98/142 (69.01%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 14  LISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLINLGSLW 73
           +I  ++     L K+EVITLT +TFSDK+KEKDTAWFVKFCVPWCKH        LG+LW
Sbjct: 11  MIILLLFIPIELVKAEVITLTPETFSDKIKEKDTAWFVKFCVPWCKH-----CKKLGNLW 70

Query: 74  EDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPRDVESLK 133
           EDLGK MEG+DEIEVGEVDCG+ ++VC+KV+IHSYPTF LFY GEEV+KYKG RDVESLK
Sbjct: 71  EDLGKAMEGDDEIEVGEVDCGTSRAVCTKVEIHSYPTFMLFYNGEEVSKYKGKRDVESLK 130

Query: 134 SFVLEEAEKAVEKAQQDSAKEL 156
           +FV+EE EKA EKAQ +  KEL
Sbjct: 131 AFVVEETEKAAEKAQLED-KEL 146

[Close]

BLAST of Carg20233 vs. TAIR 10
Match: AT1G07960.1 (PDI-like 5-1 )

HSP 1 Score: 196.4 bits (498), Expect = 1.7e-50
Identity = 98/142 (69.01%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 14  LISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLINLGSLW 73
           +I  ++     L K+EVITLT +TFSDK+KEKDTAWFVKFCVPWCKH        LG+LW
Sbjct: 11  MIILLLFIPIELVKAEVITLTPETFSDKIKEKDTAWFVKFCVPWCKH-----CKKLGNLW 70

Query: 74  EDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGEEVAKYKGPRDVESLK 133
           EDLGK MEG+DEIEVGEVDCG+ ++VC+KV+IHSYPTF LFY GEEV+KYKG RDVESLK
Sbjct: 71  EDLGKAMEGDDEIEVGEVDCGTSRAVCTKVEIHSYPTFMLFYNGEEVSKYKGKRDVESLK 130

Query: 134 SFVLEEAEKAVEKAQQDSAKEL 156
           +FV+EE EKA EKAQ +  KEL
Sbjct: 131 AFVVEETEKAAEKAQLED-KEL 146

[Close]

BLAST of Carg20233 vs. TAIR 10
Match: AT2G47470.2 (thioredoxin family protein )

HSP 1 Score: 73.6 bits (179), Expect = 1.6e-13
Identity = 48/141 (34.04%), Postives = 73/141 (51.77%), Query Frame = 0


Query: 8   AILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLI 67
           A+L+ LL+S +          +V+ LT D+F  +V  KD    V+F  PWC H       
Sbjct: 11  ALLALLLVSAVA--------DDVVVLTDDSFEKEV-GKDKGALVEFYAPWCGH-----CK 70

Query: 68  NLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGE-EVAKYKGP 127
            L   +E LG + +    + + +VDC   KSVC+K  +  YPT + F +G  E  KY+GP
Sbjct: 71  KLAPEYEKLGASFKKAKSVLIAKVDCDEQKSVCTKYGVSGYPTIQWFPKGSLEPQKYEGP 130

Query: 128 RDVESLKSFVLEEAEKAVEKA 148
           R+ E+L  +V +E    V+ A
Sbjct: 131 RNAEALAEYVNKEGGTNVKLA 137

[Close]

BLAST of Carg20233 vs. TAIR 10
Match: AT2G47470.1 (thioredoxin family protein )

HSP 1 Score: 73.6 bits (179), Expect = 1.6e-13
Identity = 48/141 (34.04%), Postives = 73/141 (51.77%), Query Frame = 0


Query: 8   AILSFLLISFIVLNTASLCKSEVITLTADTFSDKVKEKDTAWFVKFCVPWCKHWFAFFLI 67
           A+L+ LL+S +          +V+ LT D+F  +V  KD    V+F  PWC H       
Sbjct: 11  ALLALLLVSAVA--------DDVVVLTDDSFEKEV-GKDKGALVEFYAPWCGH-----CK 70

Query: 68  NLGSLWEDLGKTMEGEDEIEVGEVDCGSHKSVCSKVDIHSYPTFKLFYEGE-EVAKYKGP 127
            L   +E LG + +    + + +VDC   KSVC+K  +  YPT + F +G  E  KY+GP
Sbjct: 71  KLAPEYEKLGASFKKAKSVLIAKVDCDEQKSVCTKYGVSGYPTIQWFPKGSLEPQKYEGP 130

Query: 128 RDVESLKSFVLEEAEKAVEKA 148
           R+ E+L  +V +E    V+ A
Sbjct: 131 RNAEALAEYVNKEGGTNVKLA 137

The following BLAST results are available for this feature:

BLAST of Carg20233 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035463.1	1.4e-80	100.00	Protein disulfide-isomerase 5-1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6605525.1	1.0e-73	95.48	Protein disulfide-isomerase 5-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022947942.1	4.9e-73	94.84	protein disulfide-isomerase 5-1 [Cucurbita moschata]	[more]
XP_023007347.1	1.9e-72	93.55	protein disulfide-isomerase 5-1 [Cucurbita maxima]	[more]
XP_023532319.1	2.5e-72	94.19	protein disulfide-isomerase 5-1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg20233 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYD1	2.3e-49	69.01	Protein disulfide-isomerase 5-1 OS=Arabidopsis thaliana OX=3702 GN=PDIL5-1 PE=2 ...	[more]
Q10N04	8.8e-49	68.75	Protein disulfide isomerase-like 5-1 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q91W90	1.1e-14	35.66	Thioredoxin domain-containing protein 5 OS=Mus musculus OX=10090 GN=Txndc5 PE=1 ...	[more]
Q9VYV3	2.4e-14	32.54	Thioredoxin domain-containing protein 5 homolog OS=Drosophila melanogaster OX=72...	[more]
Q8NBS9	3.2e-14	35.34	Thioredoxin domain-containing protein 5 OS=Homo sapiens OX=9606 GN=TXNDC5 PE=1 S...	[more]

BLAST of Carg20233 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G7T1	2.4e-73	94.84	protein disulfide-isomerase 5-1 OS=Cucurbita moschata OX=3662 GN=LOC111451672 PE...	[more]
A0A6J1KYF6	9.1e-73	93.55	protein disulfide-isomerase 5-1 OS=Cucurbita maxima OX=3661 GN=LOC111499869 PE=4...	[more]
A0A0A0KFX1	2.9e-63	81.29	Thioredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290880...	[more]
A0A6J1CGL4	3.8e-63	83.23	protein disulfide isomerase-like 5-1 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A1S3CKB7	4.7e-61	78.71	protein disulfide-isomerase 5-1 OS=Cucumis melo OX=3656 GN=LOC103501930 PE=4 SV=...	[more]

BLAST of Carg20233 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07960.2	1.7e-50	69.01	PDI-like 5-1	[more]
AT1G07960.3	1.7e-50	69.01	PDI-like 5-1	[more]
AT1G07960.1	1.7e-50	69.01	PDI-like 5-1	[more]
AT2G47470.2	1.6e-13	34.04	thioredoxin family protein	[more]
AT2G47470.1	1.6e-13	34.04	thioredoxin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 137..155
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 23..155 e-value: 3.1E-27 score: 97.4
None	No IPR available	PANTHER	PTHR45672:SF3	THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5	coord: 15..153
None	No IPR available	PANTHER	PTHR45672	PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED	coord: 15..153
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 30..136 e-value: 8.0E-18 score: 64.3
IPR013766	Thioredoxin domain	PROSITE	PS51352	THIOREDOXIN_2	coord: 8..141 score: 9.000639
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 25..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20233-RA	Carg20233-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016853	isomerase activity