Carg19986 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19986
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Laccase
Location	Carg_Chr03: 4240591 .. 4243765 (-)
RNA-Seq Expression	Carg19986
Synteny	Carg19986

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATTCTTCATTCCCCACAGAGAAACGAGGCTGATAAACAACAATATAGGGAGAGAAAAGAGAGAGCTATGGGTTTAATGATTCAAGTTCTGGTTCTGGTGGCCTGCATTTTCCCAGCTTTGGTCGAATGCCGGGTTCGACATTACAAATTCAATGTAAGTCCTGGTTTTTTGATATCAATCACAAGCAAAAGGCAGGACCCTTTTGATACACAAATCCCAGCTTGTTTCTTTTGTTGTCTTGTTCCCTTATGATGATATGAAAAGGAAAGAGTTAAAGATTTTCTTTTTGTAACCTTTTCTTTCATTTTAAACATTCAAAAGGTTTCATTTGTCTTTCAGTAATCTACTGAACTTTTAAAGATCTTCGTGTCATCAACTTCATAATGTTTAGGCTAAATTTATTTGTATTATGGATTTTTTACCATTTCGTGAGATCTCACATCGGTTGGGGAGGGGAACTAAGCATTCTTTATAAGAGTGTGAAAATCTCTCCCTAGCAGACGCGTTTTAAAAACCTTGAGGGGAAGCTCGAAAGGGAAAGCCCAAAGAGAACAATATCTGTTAGTAGTAGACTTGGGCCGTTACAAATGGTATCAGAGCCAAACACTAGGCGATATGCCAGTGAGGAGGCTGAGCCCAAAGGGAGTGGACACAAGGCAGTGTGCCAGCAAAGACGCTGAGCCCCGAAGGGGGTGGATTGTGAGATCCCACATCGGTTGAGGAGGAGAATGAAACATTCTTTATAAAGGTGTAGAAATCTCTTCCTAGCAGACACGTTTTAAAAACCTTGAGGGGAAGCCCAAAAGGGAAATCCCAAAGAAGACAATATCTGCTAACGGTGGACTTAGGCTGTTACAATTTCAATCAAGTCATCAATCTTAACGGTGGACTTGGGCTGTTACAATTTCAATTAAGTCATCAATCTTTAGTGTACTAAATCTGAAATAAGGACAAATCCGACGTACTCGGGATTCAAATCTCTATTCTATATGTTAATGGCAGGTGGTTTTGAAAAATTCTACAAAACTCTGTTCAACTAAGCAAATCGTCACCGTTAACGGGAAGTTTCCAGGGCCTACTATCTATGCTAGGGAAGATGACACAGTGCTTATTAAGGTTGTTAACCATGTTCAATACAATCTTTCCATCCACTGGTAAGCCTTCCTCCAAAAAGTCCTATTTCACCTATCTTATGTGATGAACGCAAAGAATAAATGACTTATAATAGTATCTAGAGCAAAGTGAAGAATGATGCTTGCTTACCTTCTTTTTCTGATCAGGCATGGAATTCGGCAGCTTCGAACCGGTTGGGCCGATGGACCTGCATACATTACACAATGTCCAATCCCACCAGGGCAAAGTTATTTATATAACTTCACCATTACTGGCCAAAGAGGCACCCTTTTCTGGCACGCACATATTCTATGGCTGAGGGCGACTGTCCATGGTGCTGTGGTCATCTTGCCCAAACGTGGCGTGCCTTATCCATTCCCTGCTCCTCACAAAGAAGTAGTAGTTGTATTAGGTGAGCAAATTTGAAAGTTAAACAAATCTGTCTGCCCACCGCTAGCAGATATTATTCTTTTTGGGCTTTTTCTTTCGAGCTTTCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACACTCTTATAAAGGGTGTTTAGTTCTCCTCCCCAACCGATGTAGGATCTTACATTTAGCATCCTTGTTTACCTTTAATTGGACCTGACTGATTTGGTTCTAAAACATGATCCTGCTTCTGCAGCTGAGTGGTGGAAATCCGACACTGAAGCCGTGATCAACGAAGCTCTCAAGTCGGGATTAGCTCCAAATGTATCCGACGCTCACACAATCAATGGTCATTCAGGACCCATCTCAAGCTGTTCTTCACAAGGTACAAAATCAATCAGAACATAGAACAACCATTACGCATCAGAAAATTGAAAACTACTTGAAATGACATGAATATTTGTCCTCAGGGGGTTTCACGTTGCCTGTCAAGAGTGGAAATACTTACTTACTGCGCATAATCAATGCTGCACTCAATGAAGAACTGTTCTTCAAGATTGCTGGGCACAAGCTCACAGTTGTGGAGGTAGATGCTACCTATGTGAAACCATTCAAAACAGATACAATTCTAATTGCCCCAGGCCAAACAACCAATGTCCTAATAACCGCTGATCAAACCTCTGGCAAGTACTTGGTTGCAGCCTCCCCGTTCATGGACTCTCCGATCACGGTCGACAACAACACCGCCACAGCCACGCTGCATTATGCTGGCATGCTTGCTACCACCACAACGACCTTAACCGCCCCACCTCCTCAAAACGCAACTCCAGTAGCCAACAATTTCCTAAACTCTCTCAGAAGCATTAATTCAAACACCTACCCTGCACGAGTCCCATTGACCATTGATCACAACCTTTTCTTCACAGTTGGCCTTGGGATCAACCCTTGTCCCACCTGCAAAGCTGGCAACGGGAGCCGGGCAGTGGCTAGCATAAACAATGTTACATTCGTCATGCCAACCACAGCCCTATTACAAGCTCATTATTTCAACATCAATGGAGTTTTTACAACAGATTTCCCTGCTAACCCACCTCATGTTTTCAACTACACTGGCAGTGGTCCATCAAATTTGCAGACCACAAGGGGAACCAAGCTTTATAAGCTGAAATACAACGATACAGTGGAGCTTGTTTTACAAGACACCGGAATCCTTACCGCAGAAACCCACCCTGTTCATCTCCACGGGTTTAATTTCTTCGTCGTTGGAAGAGGAATCGGCAATTATGACCCTAAAAACGACCCCAAATCGTTCAATCTCATCGACCCTGTTGAGAGAAACACCGTCGGAGTGCCTTCCGGTGGATGGACGGCCATCAGATTCAGAGCTGATAATCCAGGTAGAAAAACTTCACGAACTCCTATCTAATAGATCGATGTAGTGAATGTTATTGATGATGATTTTGTTGTAGGGGTTTGGTTCATGCATTGCCATTTGGAAGTGCACACAACATGGGGATTAAAGATGGCTTTCTTGGTGGAAAATGGAAAAGGACCAAACCAATCGATCATTCCGCCTCCGAAGGACCTTCCAAAATGCTAGCCAGCCCAAGATGGCTATTAAGGAAGAAGAGACTCAAAACAGAACAACCCATTTC

mRNA sequence

CAATTCTTCATTCCCCACAGAGAAACGAGGCTGATAAACAACAATATAGGGAGAGAAAAGAGAGAGCTATGGGTTTAATGATTCAAGTTCTGGTTCTGGTGGCCTGCATTTTCCCAGCTTTGGTCGAATGCCGGGTTCGACATTACAAATTCAATGTGGTTTTGAAAAATTCTACAAAACTCTGTTCAACTAAGCAAATCGTCACCGTTAACGGGAAGTTTCCAGGGCCTACTATCTATGCTAGGGAAGATGACACAGTGCTTATTAAGGTTGTTAACCATGTTCAATACAATCTTTCCATCCACTGGCATGGAATTCGGCAGCTTCGAACCGGTTGGGCCGATGGACCTGCATACATTACACAATGTCCAATCCCACCAGGGCAAAGTTATTTATATAACTTCACCATTACTGGCCAAAGAGGCACCCTTTTCTGGCACGCACATATTCTATGGCTGAGGGCGACTGTCCATGGTGCTGTGGTCATCTTGCCCAAACGTGGCGTGCCTTATCCATTCCCTGCTCCTCACAAAGAAGTAGTAGTTGTATTAGGTGAGCAAATTTGAAAGTTAAACAAATCTGTCTGCCCACCGCTAGCAGATATTATTCTTTTTGGGCTTTTTCTTTCGAGCTTTCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACACTCTTATAAAGGGTGTTTAGTTCTCCTCCCCAACCGATGTAGGATCTTACATTTAGCATCCTTGTTTACCTTTAATTGGACCTGACTGATTTGGTTCTAAAACATGATCCTGCTTCTGCAGCTGAGTGGTGGAAATCCGACACTGAAGCCGTGATCAACGAAGCTCTCAAGTCGGGATTAGCTCCAAATGTATCCGACGCTCACACAATCAATGGTCATTCAGGACCCATCTCAAGCTGTTCTTCACAAGGGGGTTTCACGTTGCCTGTCAAGAGTGGAAATACTTACTTACTGCGCATAATCAATGCTGCACTCAATGAAGAACTGTTCTTCAAGATTGCTGGGCACAAGCTCACAGTTGTGGAGGTAGATGCTACCTATGTGAAACCATTCAAAACAGATACAATTCTAATTGCCCCAGGCCAAACAACCAATGTCCTAATAACCGCTGATCAAACCTCTGGCAAGTACTTGGTTGCAGCCTCCCCGTTCATGGACTCTCCGATCACGGTCGACAACAACACCGCCACAGCCACGCTGCATTATGCTGGCATGCTTGCTACCACCACAACGACCTTAACCGCCCCACCTCCTCAAAACGCAACTCCAGTAGCCAACAATTTCCTAAACTCTCTCAGAAGCATTAATTCAAACACCTACCCTGCACGAGTCCCATTGACCATTGATCACAACCTTTTCTTCACAGTTGGCCTTGGGATCAACCCTTGTCCCACCTGCAAAGCTGGCAACGGGAGCCGGGCAGTGGCTAGCATAAACAATGTTACATTCGTCATGCCAACCACAGCCCTATTACAAGCTCATTATTTCAACATCAATGGAGTTTTTACAACAGATTTCCCTGCTAACCCACCTCATGTTTTCAACTACACTGGCAGTGGTCCATCAAATTTGCAGACCACAAGGGGAACCAAGCTTTATAAGCTGAAATACAACGATACAGTGGAGCTTGTTTTACAAGACACCGGAATCCTTACCGCAGAAACCCACCCTGTTCATCTCCACGGGTTTAATTTCTTCGTCGTTGGAAGAGGAATCGGCAATTATGACCCTAAAAACGACCCCAAATCGTTCAATCTCATCGACCCTGTTGAGAGAAACACCGTCGGAGTGCCTTCCGGTGGATGGACGGCCATCAGATTCAGAGCTGATAATCCAGGGGTTTGGTTCATGCATTGCCATTTGGAAGTGCACACAACATGGGGATTAAAGATGGCTTTCTTGGTGGAAAATGGAAAAGGACCAAACCAATCGATCATTCCGCCTCCGAAGGACCTTCCAAAATGCTAGCCAGCCCAAGATGGCTATTAAGGAAGAAGAGACTCAAAACAGAACAACCCATTTC

Coding sequence (CDS)

ATGGGTTTAATGATTCAAGTTCTGGTTCTGGTGGCCTGCATTTTCCCAGCTTTGGTCGAATGCCGGGTTCGACATTACAAATTCAATGTGGTTTTGAAAAATTCTACAAAACTCTGTTCAACTAAGCAAATCGTCACCGTTAACGGGAAGTTTCCAGGGCCTACTATCTATGCTAGGGAAGATGACACAGTGCTTATTAAGGTTGTTAACCATGTTCAATACAATCTTTCCATCCACTGGCATGGAATTCGGCAGCTTCGAACCGGTTGGGCCGATGGACCTGCATACATTACACAATGTCCAATCCCACCAGGGCAAAGTTATTTATATAACTTCACCATTACTGGCCAAAGAGGCACCCTTTTCTGGCACGCACATATTCTATGGCTGAGGGCGACTGTCCATGGTGCTGTGGTCATCTTGCCCAAACGTGGCGTGCCTTATCCATTCCCTGCTCCTCACAAAGAAGTAGTAGTTGTATTAGGTGAGCAAATTTGA

Protein sequence

MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYAREDDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTLFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGEQI

Homology

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BLAST of Carg19986 vs. NCBI nr
Match: KAG7033783.1 (Laccase-4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 347.4 bits (890), Expect = 6.6e-92
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE
Sbjct: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT
Sbjct: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGEQI 166
           LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGEQI
Sbjct: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGEQI 165

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BLAST of Carg19986 vs. NCBI nr
Match: XP_022950756.1 (laccase-4-like [Cucurbita moschata])

HSP 1 Score: 339.0 bits (868), Expect = 2.4e-89
Identity = 160/163 (98.16%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGLMI+VLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE
Sbjct: 1   MGLMIRVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSY+YNFTITGQRGT
Sbjct: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYMYNFTITGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVL E
Sbjct: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLAE 163

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BLAST of Carg19986 vs. NCBI nr
Match: XP_023543863.1 (laccase-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 332.4 bits (851), Expect = 2.2e-87
Identity = 157/163 (96.32%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGLMI+VLVLVACIFPA VECRVRHYKFNVVLKN+TKLCS+KQIVTVNGKFPGPTIYARE
Sbjct: 1   MGLMIRVLVLVACIFPAFVECRVRHYKFNVVLKNTTKLCSSKQIVTVNGKFPGPTIYARE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIP GQSYLYNFTITGQRGT
Sbjct: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPSGQSYLYNFTITGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVL E
Sbjct: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLAE 163

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BLAST of Carg19986 vs. NCBI nr
Match: XP_022978138.1 (laccase-4-like [Cucurbita maxima])

HSP 1 Score: 330.5 bits (846), Expect = 8.4e-87
Identity = 156/163 (95.71%), Postives = 159/163 (97.55%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGLMI+ LVLVACIFPALVECRVRHYKFNVVLKN+TKLCS+KQIVTVNGKFPGPTIY RE
Sbjct: 1   MGLMIRFLVLVACIFPALVECRVRHYKFNVVLKNTTKLCSSKQIVTVNGKFPGPTIYVRE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIP GQSYLYNFTITGQRGT
Sbjct: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPSGQSYLYNFTITGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVL E
Sbjct: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLAE 163

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BLAST of Carg19986 vs. NCBI nr
Match: XP_038881079.1 (laccase-4-like [Benincasa hispida])

HSP 1 Score: 324.3 bits (830), Expect = 6.0e-85
Identity = 152/163 (93.25%), Postives = 159/163 (97.55%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGL+I+VLVLVACIFPALVECRVRHYKF+VVLKN+TKLCS+KQIVTVNGKFPGPTIYARE
Sbjct: 1   MGLIIRVLVLVACIFPALVECRVRHYKFDVVLKNTTKLCSSKQIVTVNGKFPGPTIYARE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLI VVNHVQYNLSIHWHG+RQLRTGWADGPAYITQCPIP G+SYLYNFTITGQRGT
Sbjct: 61  DDTVLINVVNHVQYNLSIHWHGVRQLRTGWADGPAYITQCPIPSGKSYLYNFTITGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           L WHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVL E
Sbjct: 121 LLWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLAE 163

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BLAST of Carg19986 vs. ExPASy Swiss-Prot
Match: Q6ID18 (Laccase-10 OS=Arabidopsis thaliana OX=3702 GN=LAC10 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 3.0e-71
Identity = 121/160 (75.62%), Postives = 141/160 (88.12%), Query Frame = 0


Query: 5   IQVLVLVACI-FPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYAREDDT 64
           I++LVL A + FPA V   +R Y FNVV K  T++CSTKQIVTVNGKFPGPTIYA EDDT
Sbjct: 5   IRILVLFALLAFPACVHGAIRKYTFNVVTKQVTRICSTKQIVTVNGKFPGPTIYANEDDT 64

Query: 65  VLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTLFW 124
           +L+ VVN+V+YN+SIHWHGIRQLRTGWADGPAYITQCPI PG SY+YNFT+TGQRGTL+W
Sbjct: 65  ILVNVVNNVKYNVSIHWHGIRQLRTGWADGPAYITQCPIKPGHSYVYNFTVTGQRGTLWW 124

Query: 125 HAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           HAH+LWLRATVHGA+VILPK G+PYPFP PH+E V++LGE
Sbjct: 125 HAHVLWLRATVHGAIVILPKLGLPYPFPKPHREEVIILGE 164

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BLAST of Carg19986 vs. ExPASy Swiss-Prot
Match: O80434 (Laccase-4 OS=Arabidopsis thaliana OX=3702 GN=IRX12 PE=2 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 8.7e-71
Identity = 123/162 (75.93%), Postives = 142/162 (87.65%), Query Frame = 0


Query: 4   MIQVLVLVA--CIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARED 63
           M+  L LV+   +FPA  E  VRHYKFNVV+KN T+LCS+K  VTVNG++PGPTIYARED
Sbjct: 5   MVWFLFLVSFFSVFPAPSESMVRHYKFNVVMKNVTRLCSSKPTVTVNGRYPGPTIYARED 64

Query: 64  DTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTL 123
           DT+LIKVVNHV+YN+SIHWHG+RQ+RTGWADGPAYITQCPI PGQ Y YN+T+TGQRGTL
Sbjct: 65  DTLLIKVVNHVKYNVSIHWHGVRQVRTGWADGPAYITQCPIQPGQVYTYNYTLTGQRGTL 124

Query: 124 FWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           +WHAHILWLRATV+GA+VILPKRGVPYPFP P  E V+VLGE
Sbjct: 125 WWHAHILWLRATVYGALVILPKRGVPYPFPKPDNEKVIVLGE 166

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BLAST of Carg19986 vs. ExPASy Swiss-Prot
Match: Q0IQU1 (Laccase-22 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC22 PE=2 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 3.7e-69
Identity = 117/161 (72.67%), Postives = 136/161 (84.47%), Query Frame = 0


Query: 5   IQVLVLVACIFPALVECR--VRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYAREDD 64
           + +L++ AC     +      RHYKFNVV++N T+LCSTK I+TVNGKFPGPT+YARE D
Sbjct: 10  LSLLLMAACFLLQALSAHAITRHYKFNVVMRNMTRLCSTKPILTVNGKFPGPTLYAREGD 69

Query: 65  TVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTLF 124
            VL+KVVNHV +N++IHWHG+RQ+RTGW DGPAYITQCPI PG S+LYNFTITGQRGTL 
Sbjct: 70  NVLVKVVNHVAHNVTIHWHGVRQIRTGWYDGPAYITQCPIQPGSSFLYNFTITGQRGTLL 129

Query: 125 WHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           WHAHI WLRATVHGA+VILPK GVPYPFPAPHKE V+VLGE
Sbjct: 130 WHAHINWLRATVHGAIVILPKLGVPYPFPAPHKEAVIVLGE 170

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BLAST of Carg19986 vs. ExPASy Swiss-Prot
Match: Q1PDH6 (Laccase-16 OS=Arabidopsis thaliana OX=3702 GN=LAC16 PE=2 SV=2)

HSP 1 Score: 259.2 bits (661), Expect = 3.1e-68
Identity = 117/148 (79.05%), Postives = 132/148 (89.19%), Query Frame = 0


Query: 16  PALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYAREDDTVLIKVVNHVQYN 75
           P  V   +RHYKFN V+ N+TKLCS+K IVTVNG+FPGPTI ARE DT+LIKVVNHV+YN
Sbjct: 22  PTTVHSIIRHYKFN-VMTNTTKLCSSKPIVTVNGQFPGPTIVAREGDTILIKVVNHVKYN 81

Query: 76  LSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTLFWHAHILWLRATVH 135
           +SIHWHGIRQLRTGWADGPAYITQCPI PGQ+YL+NFT+TGQRGTL+WHAHILWLRATVH
Sbjct: 82  VSIHWHGIRQLRTGWADGPAYITQCPIQPGQNYLHNFTLTGQRGTLWWHAHILWLRATVH 141

Query: 136 GAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           GA+VILPK GVPYPFP P+KE  +VL E
Sbjct: 142 GAIVILPKLGVPYPFPKPYKEKTIVLSE 168

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BLAST of Carg19986 vs. ExPASy Swiss-Prot
Match: Q5N9X2 (Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 3.7e-61
Identity = 105/158 (66.46%), Postives = 132/158 (83.54%), Query Frame = 0


Query: 8   LVLVACIFPALVECR--VRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYAREDDTVL 67
           L+L A +   +V+ +   RHY+FNV + N+T+LC+TK +VTVNG+ PGP + ARE D V+
Sbjct: 13  LLLAASLLLLIVQAQGITRHYEFNVQMANATRLCNTKSMVTVNGQCPGPELVAREGDRVV 72

Query: 68  IKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTLFWHA 127
           I+V N+V +N+S+HWHG+RQ+RTGWADGPAYITQCPI  GQSY+YNFT+ GQRGTL+WHA
Sbjct: 73  IRVTNNVAHNISLHWHGVRQVRTGWADGPAYITQCPIQTGQSYVYNFTVAGQRGTLWWHA 132

Query: 128 HILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           HI WLRATV+GA+VILPK GVPYPFPAPHKEV V+ GE
Sbjct: 133 HISWLRATVYGALVILPKLGVPYPFPAPHKEVPVIFGE 170

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BLAST of Carg19986 vs. ExPASy TrEMBL
Match: A0A6J1GFQ1 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453763 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.1e-89
Identity = 160/163 (98.16%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGLMI+VLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE
Sbjct: 1   MGLMIRVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSY+YNFTITGQRGT
Sbjct: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYMYNFTITGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVL E
Sbjct: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLAE 163

[Close]

BLAST of Carg19986 vs. ExPASy TrEMBL
Match: A0A6J1IS57 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111478208 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.1e-87
Identity = 156/163 (95.71%), Postives = 159/163 (97.55%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGLMI+ LVLVACIFPALVECRVRHYKFNVVLKN+TKLCS+KQIVTVNGKFPGPTIY RE
Sbjct: 1   MGLMIRFLVLVACIFPALVECRVRHYKFNVVLKNTTKLCSSKQIVTVNGKFPGPTIYVRE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIP GQSYLYNFTITGQRGT
Sbjct: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPSGQSYLYNFTITGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVL E
Sbjct: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLAE 163

[Close]

BLAST of Carg19986 vs. ExPASy TrEMBL
Match: A0A6J1DCH3 (Laccase OS=Momordica charantia OX=3673 GN=LOC111019466 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.0e-85
Identity = 152/163 (93.25%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGL+I+VLVLVACIFPALVECRVRHYKF+VVLKN+TKLCS+KQIVTVNGKFPGPTIYARE
Sbjct: 38  MGLIIRVLVLVACIFPALVECRVRHYKFDVVLKNTTKLCSSKQIVTVNGKFPGPTIYARE 97

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVN+VQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSY+YNFTITGQRGT
Sbjct: 98  DDTVLIKVVNNVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYVYNFTITGQRGT 157

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           L WHAHILWLRATVHGA+VILPK GVPYPFPAPHKEVVVVL E
Sbjct: 158 LLWHAHILWLRATVHGALVILPKLGVPYPFPAPHKEVVVVLAE 200

[Close]

BLAST of Carg19986 vs. ExPASy TrEMBL
Match: A0A6J1HUA3 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111466237 PE=3 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.6e-83
Identity = 148/163 (90.80%), Postives = 157/163 (96.32%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MGL+I+VLVLVACIFPA VECRVR Y F+VVLKN+TKLCS+KQIVTVNGKFPGPTIYARE
Sbjct: 1   MGLIIRVLVLVACIFPAFVECRVRRYTFDVVLKNTTKLCSSKQIVTVNGKFPGPTIYARE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAY+TQCPIP GQSYLYNFT+TGQRGT
Sbjct: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYVTQCPIPSGQSYLYNFTVTGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           LFWHAHILWLRATVHGA+VILPK GVPYPFPAPHKEVV+VL E
Sbjct: 121 LFWHAHILWLRATVHGALVILPKLGVPYPFPAPHKEVVIVLAE 163

[Close]

BLAST of Carg19986 vs. ExPASy TrEMBL
Match: A0A6J1EVQ0 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111438226 PE=3 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.0e-82
Identity = 145/163 (88.96%), Postives = 157/163 (96.32%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MG++I+VLVLVACIFPA +ECRV+ Y F+VVLKN+TKLCS+KQIVTVNGKFPGPTIYARE
Sbjct: 1   MGIIIRVLVLVACIFPAFIECRVQRYTFDVVLKNTTKLCSSKQIVTVNGKFPGPTIYARE 60

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
           DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAY+TQCPIP GQSYLYNFT+TGQRGT
Sbjct: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYVTQCPIPSGQSYLYNFTVTGQRGT 120

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           LFWHAHILWLRATVHGA+VILPK GVPYPFPAPHKEVV+VL E
Sbjct: 121 LFWHAHILWLRATVHGALVILPKLGVPYPFPAPHKEVVIVLAE 163

[Close]

BLAST of Carg19986 vs. TAIR 10
Match: AT5G01190.1 (laccase 10 )

HSP 1 Score: 269.2 bits (687), Expect = 2.1e-72
Identity = 121/160 (75.62%), Postives = 141/160 (88.12%), Query Frame = 0


Query: 5   IQVLVLVACI-FPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYAREDDT 64
           I++LVL A + FPA V   +R Y FNVV K  T++CSTKQIVTVNGKFPGPTIYA EDDT
Sbjct: 5   IRILVLFALLAFPACVHGAIRKYTFNVVTKQVTRICSTKQIVTVNGKFPGPTIYANEDDT 64

Query: 65  VLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTLFW 124
           +L+ VVN+V+YN+SIHWHGIRQLRTGWADGPAYITQCPI PG SY+YNFT+TGQRGTL+W
Sbjct: 65  ILVNVVNNVKYNVSIHWHGIRQLRTGWADGPAYITQCPIKPGHSYVYNFTVTGQRGTLWW 124

Query: 125 HAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           HAH+LWLRATVHGA+VILPK G+PYPFP PH+E V++LGE
Sbjct: 125 HAHVLWLRATVHGAIVILPKLGLPYPFPKPHREEVIILGE 164

[Close]

BLAST of Carg19986 vs. TAIR 10
Match: AT2G38080.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 267.7 bits (683), Expect = 6.2e-72
Identity = 123/162 (75.93%), Postives = 142/162 (87.65%), Query Frame = 0


Query: 4   MIQVLVLVA--CIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARED 63
           M+  L LV+   +FPA  E  VRHYKFNVV+KN T+LCS+K  VTVNG++PGPTIYARED
Sbjct: 5   MVWFLFLVSFFSVFPAPSESMVRHYKFNVVMKNVTRLCSSKPTVTVNGRYPGPTIYARED 64

Query: 64  DTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTL 123
           DT+LIKVVNHV+YN+SIHWHG+RQ+RTGWADGPAYITQCPI PGQ Y YN+T+TGQRGTL
Sbjct: 65  DTLLIKVVNHVKYNVSIHWHGVRQVRTGWADGPAYITQCPIQPGQVYTYNYTLTGQRGTL 124

Query: 124 FWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           +WHAHILWLRATV+GA+VILPKRGVPYPFP P  E V+VLGE
Sbjct: 125 WWHAHILWLRATVYGALVILPKRGVPYPFPKPDNEKVIVLGE 166

[Close]

BLAST of Carg19986 vs. TAIR 10
Match: AT5G03260.1 (laccase 11 )

HSP 1 Score: 231.5 bits (589), Expect = 4.9e-61
Identity = 103/163 (63.19%), Postives = 130/163 (79.75%), Query Frame = 0


Query: 1   MGLMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYARE 60
           MG +     L+A +  + V+  V+ Y+F+V +KN +++C+ K IVTVNG FPGPT+YARE
Sbjct: 3   MGFLFLFCYLLAFLGYSPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYARE 62

Query: 61  DDTVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGT 120
            D V+I V NHVQYN+SIHWHG++Q R GWADGPAYITQCPI  GQSYLY+F +TGQRGT
Sbjct: 63  GDRVIINVTNHVQYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQRGT 122

Query: 121 LFWHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           L+WHAHILWLRATV+GA+VILP  G PYPFP P++E  ++LGE
Sbjct: 123 LWWHAHILWLRATVYGAIVILPAPGKPYPFPQPYQESNIILGE 165

[Close]

BLAST of Carg19986 vs. TAIR 10
Match: AT2G29130.1 (laccase 2 )

HSP 1 Score: 224.9 bits (572), Expect = 4.6e-59
Identity = 101/161 (62.73%), Postives = 126/161 (78.26%), Query Frame = 0


Query: 3   LMIQVLVLVACIFPALVECRVRHYKFNVVLKNSTKLCSTKQIVTVNGKFPGPTIYAREDD 62
           L++  L  ++    A      RHY+F++ LKN T+LC TK IVTVNGKFPGP + ARE D
Sbjct: 9   LLVAFLFAISYNIDAASAGITRHYQFDIQLKNITRLCKTKTIVTVNGKFPGPRVTAREGD 68

Query: 63  TVLIKVVNHVQYNLSIHWHGIRQLRTGWADGPAYITQCPIPPGQSYLYNFTITGQRGTLF 122
            + IKVVNHV  N+SIHWHGIRQLR+GWADGP+Y+TQCPI  GQSY+YNFT+TGQRGTL+
Sbjct: 69  NLQIKVVNHVSNNISIHWHGIRQLRSGWADGPSYVTQCPIRMGQSYVYNFTVTGQRGTLW 128

Query: 123 WHAHILWLRATVHGAVVILPKRGVPYPFPAPHKEVVVVLGE 164
           WHAHI W+RATV+G ++ILPK   PYPFP P+K+V ++ GE
Sbjct: 129 WHAHIQWMRATVYGPLIILPKLHQPYPFPKPYKQVPILFGE 169

[Close]

BLAST of Carg19986 vs. TAIR 10
Match: AT5G58910.1 (laccase 16 )

HSP 1 Score: 223.0 bits (567), Expect = 1.8e-58
Identity = 101/132 (76.52%), Postives = 115/132 (87.12%), Query Frame = 0


Query: 32  LKNSTKLCSTKQIVTVNGKFPGPTIYAREDDTVLIKVVNHVQYNLSIHWHGIRQLRTGWA 91
           + N+TKLCS+K IVTVNG+FPGPTI ARE DT+LIKVVNHV+YN+SIHW       TGWA
Sbjct: 1   MTNTTKLCSSKPIVTVNGQFPGPTIVAREGDTILIKVVNHVKYNVSIHW-------TGWA 60

Query: 92  DGPAYITQCPIPPGQSYLYNFTITGQRGTLFWHAHILWLRATVHGAVVILPKRGVPYPFP 151
           DGPAYITQCPI PGQ+YL+NFT+TGQRGTL+WHAHILWLRATVHGA+VILPK GVPYPFP
Sbjct: 61  DGPAYITQCPIQPGQNYLHNFTLTGQRGTLWWHAHILWLRATVHGAIVILPKLGVPYPFP 120

Query: 152 APHKEVVVVLGE 164
            P+KE  +VL E
Sbjct: 121 KPYKEKTIVLSE 125

The following BLAST results are available for this feature:

BLAST of Carg19986 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033783.1	6.6e-92	100.00	Laccase-4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022950756.1	2.4e-89	98.16	laccase-4-like [Cucurbita moschata]	[more]
XP_023543863.1	2.2e-87	96.32	laccase-4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022978138.1	8.4e-87	95.71	laccase-4-like [Cucurbita maxima]	[more]
XP_038881079.1	6.0e-85	93.25	laccase-4-like [Benincasa hispida]	[more]

BLAST of Carg19986 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6ID18	3.0e-71	75.63	Laccase-10 OS=Arabidopsis thaliana OX=3702 GN=LAC10 PE=2 SV=1	[more]
O80434	8.7e-71	75.93	Laccase-4 OS=Arabidopsis thaliana OX=3702 GN=IRX12 PE=2 SV=2	[more]
Q0IQU1	3.7e-69	72.67	Laccase-22 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC22 PE=2 SV=2	[more]
Q1PDH6	3.1e-68	79.05	Laccase-16 OS=Arabidopsis thaliana OX=3702 GN=LAC16 PE=2 SV=2	[more]
Q5N9X2	3.7e-61	66.46	Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1	[more]

BLAST of Carg19986 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GFQ1	1.1e-89	98.16	Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453763 PE=3 SV=1	[more]
A0A6J1IS57	4.1e-87	95.71	Laccase OS=Cucurbita maxima OX=3661 GN=LOC111478208 PE=3 SV=1	[more]
A0A6J1DCH3	5.0e-85	93.25	Laccase OS=Momordica charantia OX=3673 GN=LOC111019466 PE=3 SV=1	[more]
A0A6J1HUA3	1.6e-83	90.80	Laccase OS=Cucurbita maxima OX=3661 GN=LOC111466237 PE=3 SV=1	[more]
A0A6J1EVQ0	1.0e-82	88.96	Laccase OS=Cucurbita moschata OX=3662 GN=LOC111438226 PE=3 SV=1	[more]

BLAST of Carg19986 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01190.1	2.1e-72	75.63	laccase 10	[more]
AT2G38080.1	6.2e-72	75.93	Laccase/Diphenol oxidase family protein	[more]
AT5G03260.1	4.9e-61	63.19	laccase 11	[more]
AT2G29130.1	4.6e-59	62.73	laccase 2	[more]
AT5G58910.1	1.8e-58	76.52	laccase 16	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 33..143 e-value: 4.6E-42 score: 142.8
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 16..142 e-value: 2.8E-36 score: 126.9
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 16..162
None	No IPR available	PANTHER	PTHR11709:SF319	LACCASE-16	coord: 5..163
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 5..163
IPR034288	Laccase, first cupredoxin domain	CDD	cd13849	CuRO_1_LCC_plant	coord: 26..142 e-value: 8.97243E-75 score: 217.512

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19986-RA	Carg19986-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046274	lignin catabolic process
cellular_component	GO:0048046	apoplast
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0052716	hydroquinone:oxygen oxidoreductase activity