Carg19899 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19899
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionheat stress transcription factor A-4c-like
LocationCarg_Chr13: 6973433 .. 6976750 (+)
RNA-Seq ExpressionCarg19899
SyntenyCarg19899
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCTTTTCCCCCCTCCTCTTTTTGTTTGTTGAATCTAAGGGCATTTTTTAATCGTCTTTCGTTTCCACTTTTTTGTTATTTCAATTGAATTGATTGGATTTCATTAAGTATTTTATTACTTCAGTTCAAACCGTTAAACTCCGTTCCGCAACTCTCTGTTCTTTTGTCTTCTTTAGGCTATGTTGAGCAAGTTCTACTCGAGTTGACTGGAGAAGGGTCGATGCAAAATTCGTATTTTCTTTCAAAATCCCATATGGGAAATCGATAATTCTGAATTGGGTTTGCCTGGAAATCAAGTTTTGATGGAAATTTCTGGGATTCACCGGTGTTTTTGTGATTTAAATGAAGACCCAACTCGGGGTTTTTGCTTTTGAGGTTTGAAGTTCTTTGGTGGATTATCGTTGTTGTTCTTGAAGTTTGATGTGGTGAATCTGTTAAGAGTTGTATTGATATTTTTGTAAGGATTTTGATACGCGATCTTCTGTTTGCAATTTGGTGGATTGGGAGTTGTTAATGTACAGCTTTTGGTTTCTTAATTTGGGGGTTCTTATTTTGGGGACTGGAGAGGTTAGGATATAGAATGGATGAAGCTCAGGGAAGCGGCTTGACTTCGTTGCCTCCATTTTTAGTCAAAACATATGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCAAGTGATAAAAGCTTTGTTGTTTGGAAACCACTGGAGTTTTCATCAGTTTTGTTGCCTAAATTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACATATGTAAGATGATAAGTTTTTCATTTACTTGCTTATTGTTGTGATTGTGTCCTTGTTTTTGTTTTCTGAACATTTTGTTCATTACTTTAGGGGTTCAAGAAGGTGGATCCTGATCAATGGGAATTTGCCAATGATGATTTTGTTAGAGGTGAGCAACACCTGATGAAGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCCCCATGGGCAAGGAGTATCTCCTCCGCTAACTGAAGTCGAGAGAAAGAGTTTCGAGGATAACATCGAGACGCTGAAACGGGATAAAGAGCAGCTTCTTCTCGAGTTGCGGAAACACGAACAAGAGTATCAAGGAGTTGTGTTGCAAATGCAGAATTTGAAAGATCGCTTTCAATGTGTTCAACAAGGCATGCAATTATTTATCAGTCTGATAGCTCGTTTTTTGCATAAACCAGGACTTCGCTTGGATCTTCTGCCACAATTGGAAACTTCTGATAGAAAGAGGAGATTGCCTAGAGTTTCTTACAACAATAGTGAAGATAACCTTGAGGATAATCAGATGGGGACAACTCAAACCATTAGTAGAGAAAATAGGGATTGTAGTTTTGATCCAATTTTGAAAGAAGAACAGTTTGAACTTGTTGAGACATCCCTAACCTTTTGGGAAGGCATTATCCATAGTTATGGTCAAACAATTAGTCCACTTGATTCCAGCTCAAACCTGGAGTTGGGTGGATCTGTAAGTCATGCCAGTAGCCCTGCTGTAACTTGCAGGCAAGTTAGTGAGGAGCTTCGGTGTAAATCACCAGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAGGCAGCTGGAGTCAAAGCTCCAGTACCAACTGGTGTCAATGATGTTTTCTGGCAGCAATTCTTGACGGAGAATCCTGGTTCATCTGACCCACAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAATCGAAGAAAACAGACCGAGCGATCAAGGAAATTTTTGGTGGAACACGAGGAGTGTAAATAATGTTGTAGAACAGATAGGGAACCTTGCTCCAGCAGAGAAATTTTCATAGTAGGTTGATTGTTTTTCTGAACTACTGATTTTCCTTAGCTCACATGTAACATAATTTATTATAAAATGGAACATTTGGGCTCTCATCTATGTCTTTCTTCACATGTTCTCATTGGTTTTGATTCCCTCATAATGCTTGACTTGTTTAGAGAATTAGTGGCTGTACCTATGAATGAACAATAATTTTGACTACTTTGGTTGTGTAATAGGGAATAGGCATTAGTGTTCGATCATTGTATAGTATGCAAGTGATCATTATTAGGAATTTGAGTCCCGCGGATCTATATTTATATGTTAGTTTATTATTCGTTATTATAGTATTCTACATCGGCTAATTAAGGTGTTGATCATGGGTTTATAAGTGAGGAACATTATCTCCATTGGTACGAGACCTTTTGGAGAAACCAAAAGCAATTGTGGAGAGTTGAGGTTCCTAACCTTGCATCTTGGATTAGTTTGTTTCAATCTTCTGACTTTCATGCATTTGTTTCCTGTTTCCTATTGTCTTTGAATATTGGTGGTTTATCTTTTGTTATGGAGATATCAGTGAAGTATCATGAAACCCTGTAACATGAGAAGAAAGAGTTTGTATTCTTGTTTAATATTTCATTAACTTTAGAGAAGGTTCTATTTGTTCGAGGACTTGAATGATGATTTGTTGAGGACTTCCAACAGGCTGCAAAACGGGGGAAGGAGATTGCTTTGGCCCTTGAGGATTTACACAGGCTTTCGTGCGTGCTTGTTTCATGTAAAGTATCTCTTATCTGTACATAGTGATATGACATCTGAATAAAAGGTATCCATTTTGCAACGTGAAGTGTTCAAGTATCTCATATCTGATGTTCGTCTTGTGTCATCTGGAGGGGCATACGTTCATGATTTTTGGTATCACTTCCTGATTCATTTGTCTATTGTAGGTAACTGTGCACGTTGAGAGGGCGATTTTGAACTACAATAAGTTAATGAAGGTGAAGGATAGCCCTACTGCTGCTTCCACTCTGTGAAATTGGCTTGGAATCTGCTAAAAGAAACATCCAGATCTCACCTGATTGCCACTTGTCTTTGGATACTGGGGTACACGACCAAATTGAATCCAAACACTCTGCCACTTTGCCATGGTTACTATGGTGATTACTATCAGAAAGAACACAGTTTAGGACCTTGATTATATCCTCTAGAGTTGTGATTTTCCTCGGGTTGTTTGGTGTTGGTTTCTTTAAGATGTTTGGCTAATAGCTATGCGAGCTCTCAAAGATGCATGAAGTAGATTGAGGAGCTTCTCCACCATCGACCTTTGTGTGAGAAAAGACTGTTTCAATTTCCACCATCCACGTTTGTGTGAGAAAAAACTGTTTCTATGAGATCCCACGTAGGTTGGAGAGGGAAACGAAACATTCCTTATAAAAGTGTGAAAA

mRNA sequence

TCTCTTTTCCCCCCTCCTCTTTTTGTTTGTTGAATCTAAGGGCATTTTTTAATCGTCTTTCGTTTCCACTTTTTTGTTATTTCAATTGAATTGATTGGATTTCATTAAGTATTTTATTACTTCAGTTCAAACCGTTAAACTCCGTTCCGCAACTCTCTGTTCTTTTGTCTTCTTTAGGCTATGTTGAGCAAGTTCTACTCGAGTTGACTGGAGAAGGGTCGATGCAAAATTCGTATTTTCTTTCAAAATCCCATATGGGAAATCGATAATTCTGAATTGGGTTTGCCTGGAAATCAAGTTTTGATGGAAATTTCTGGGATTCACCGGTGTTTTTGTGATTTAAATGAAGACCCAACTCGGGGTTTTTGCTTTTGAGGTTTGAAGTTCTTTGGTGGATTATCGTTGTTGTTCTTGAAGTTTGATGTGGTGAATCTGTTAAGAGTTGTATTGATATTTTTGTAAGGATTTTGATACGCGATCTTCTGTTTGCAATTTGGTGGATTGGGAGTTGTTAATGTACAGCTTTTGGTTTCTTAATTTGGGGGTTCTTATTTTGGGGACTGGAGAGGTTAGGATATAGAATGGATGAAGCTCAGGGAAGCGGCTTGACTTCGTTGCCTCCATTTTTAGTCAAAACATATGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCAAGTGATAAAAGCTTTGTTGTTTGGAAACCACTGGAGTTTTCATCAGTTTTGTTGCCTAAATTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACATATGGGTTCAAGAAGGTGGATCCTGATCAATGGGAATTTGCCAATGATGATTTTGTTAGAGGTGAGCAACACCTGATGAAGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCCCCATGGGCAAGGAGTATCTCCTCCGCTAACTGAAGTCGAGAGAAAGAGTTTCGAGGATAACATCGAGACGCTGAAACGGGATAAAGAGCAGCTTCTTCTCGAGTTGCGGAAACACGAACAAGAGTATCAAGGAGTTGTGTTGCAAATGCAGAATTTGAAAGATCGCTTTCAATGTGTTCAACAAGGCATGCAATTATTTATCAGTCTGATAGCTCGTTTTTTGCATAAACCAGGACTTCGCTTGGATCTTCTGCCACAATTGGAAACTTCTGATAGAAAGAGGAGATTGCCTAGAGTTTCTTACAACAATAGTGAAGATAACCTTGAGGATAATCAGATGGGGACAACTCAAACCATTAGTAGAGAAAATAGGGATTGTAGTTTTGATCCAATTTTGAAAGAAGAACAGTTTGAACTTGTTGAGACATCCCTAACCTTTTGGGAAGGCATTATCCATAGTTATGGTCAAACAATTAGTCCACTTGATTCCAGCTCAAACCTGGAGTTGGGTGGATCTGTAAGTCATGCCAGTAGCCCTGCTGTAACTTGCAGGCAAGTTAGTGAGGAGCTTCGGTGTAAATCACCAGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAGGCAGCTGGAGTCAAAGCTCCAGTACCAACTGGTGTCAATGATGTTTTCTGGCAGCAATTCTTGACGGAGAATCCTGGTTCATCTGACCCACAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAATCGAAGAAAACAGACCGAGCGATCAAGGAAATTTTTGGTGGAACACGAGGAGTGTAAATAATGTTGTAGAACAGATAGGGAACCTTGCTCCAGCAGAGAAATTTTCATAGCTGCAAAACGGGGGAAGGAGATTGCTTTGGCCCTTGAGGATTTACACAGGCTTTCGTGCGTGCTTGTTTCATGTAACTGTGCACGTTGAGAGGGCGATTTTGAACTACAATAAGTTAATGAAGGTGAAGGATAGCCCTACTGCTGCTTCCACTCTGTGAAATTGGCTTGGAATCTGCTAAAAGAAACATCCAGATCTCACCTGATTGCCACTTGTCTTTGGATACTGGGGTACACGACCAAATTGAATCCAAACACTCTGCCACTTTGCCATGGTTACTATGGTGATTACTATCAGAAAGAACACAGTTTAGGACCTTGATTATATCCTCTAGAGTTGTGATTTTCCTCGGGTTGTTTGGTGTTGGTTTCTTTAAGATGTTTGGCTAATAGCTATGCGAGCTCTCAAAGATGCATGAAGTAGATTGAGGAGCTTCTCCACCATCGACCTTTGTGTGAGAAAAGACTGTTTCAATTTCCACCATCCACGTTTGTGTGAGAAAAAACTGTTTCTATGAGATCCCACGTAGGTTGGAGAGGGAAACGAAACATTCCTTATAAAAGTGTGAAAA

Coding sequence (CDS)

ATGGATGAAGCTCAGGGAAGCGGCTTGACTTCGTTGCCTCCATTTTTAGTCAAAACATATGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCAAGTGATAAAAGCTTTGTTGTTTGGAAACCACTGGAGTTTTCATCAGTTTTGTTGCCTAAATTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACATATGGGTTCAAGAAGGTGGATCCTGATCAATGGGAATTTGCCAATGATGATTTTGTTAGAGGTGAGCAACACCTGATGAAGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCCCCATGGGCAAGGAGTATCTCCTCCGCTAACTGAAGTCGAGAGAAAGAGTTTCGAGGATAACATCGAGACGCTGAAACGGGATAAAGAGCAGCTTCTTCTCGAGTTGCGGAAACACGAACAAGAGTATCAAGGAGTTGTGTTGCAAATGCAGAATTTGAAAGATCGCTTTCAATGTGTTCAACAAGGCATGCAATTATTTATCAGTCTGATAGCTCGTTTTTTGCATAAACCAGGACTTCGCTTGGATCTTCTGCCACAATTGGAAACTTCTGATAGAAAGAGGAGATTGCCTAGAGTTTCTTACAACAATAGTGAAGATAACCTTGAGGATAATCAGATGGGGACAACTCAAACCATTAGTAGAGAAAATAGGGATTGTAGTTTTGATCCAATTTTGAAAGAAGAACAGTTTGAACTTGTTGAGACATCCCTAACCTTTTGGGAAGGCATTATCCATAGTTATGGTCAAACAATTAGTCCACTTGATTCCAGCTCAAACCTGGAGTTGGGTGGATCTGTAAGTCATGCCAGTAGCCCTGCTGTAACTTGCAGGCAAGTTAGTGAGGAGCTTCGGTGTAAATCACCAGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAGGCAGCTGGAGTCAAAGCTCCAGTACCAACTGGTGTCAATGATGTTTTCTGGCAGCAATTCTTGACGGAGAATCCTGGTTCATCTGACCCACAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAATCGAAGAAAACAGACCGAGCGATCAAGGAAATTTTTGGTGGAACACGAGGAGTGTAAATAATGTTGTAGAACAGATAGGGAACCTTGCTCCAGCAGAGAAATTTTCATAG

Protein sequence

MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQLFISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRDCSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQVSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSSDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS
Homology
BLAST of Carg19899 vs. NCBI nr
Match: XP_022927503.1 (heat stress transcription factor A-4c-like [Cucurbita moschata] >XP_022927504.1 heat stress transcription factor A-4c-like [Cucurbita moschata] >KAG7019556.1 Heat stress transcription factor A-4a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 822.8 bits (2124), Expect = 1.3e-234
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 412
           DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411

BLAST of Carg19899 vs. NCBI nr
Match: XP_023519250.1 (heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo] >XP_023519251.1 heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 813.9 bits (2101), Expect = 6.2e-232
Identity = 405/411 (98.54%), Postives = 409/411 (99.51%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFEDNIETLKRDKEQL+LELRKH+QEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISREN D
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFEL+ETS+ FWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 412
           DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411

BLAST of Carg19899 vs. NCBI nr
Match: KAG6583940.1 (Heat stress transcription factor A-4a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 787.3 bits (2032), Expect = 6.2e-224
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRS 394
           DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRS
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRS 393

BLAST of Carg19899 vs. NCBI nr
Match: XP_023000762.1 (heat stress transcription factor A-4a-like [Cucurbita maxima] >XP_023000763.1 heat stress transcription factor A-4a-like [Cucurbita maxima])

HSP 1 Score: 754.6 bits (1947), Expect = 4.5e-214
Identity = 376/388 (96.91%), Postives = 383/388 (98.71%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKV P+QWEFANDDFVRG+QHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVHPEQWEFANDDFVRGKQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFED+IETLKRDKEQ+LLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDDIETLKRDKEQILLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FISLIAR LHKPG RLDLLPQLETSDRKRRLPRVSYNNSEDN+EDNQMGTTQTISREN D
Sbjct: 181 FISLIARLLHKPGRRLDLLPQLETSDRKRRLPRVSYNNSEDNIEDNQMGTTQTISRENMD 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFELVETSLTFWEGI+HSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIVHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPES+ASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESIASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFW 389
           DPQEVQSARKDSDVIIEENR SDQGNFW
Sbjct: 361 DPQEVQSARKDSDVIIEENRRSDQGNFW 388

BLAST of Carg19899 vs. NCBI nr
Match: XP_038895068.1 (heat stress transcription factor A-4a [Benincasa hispida] >XP_038895070.1 heat stress transcription factor A-4a [Benincasa hispida])

HSP 1 Score: 703.0 bits (1813), Expect = 1.5e-198
Identity = 351/410 (85.61%), Postives = 372/410 (90.73%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW PLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGF+KVDP+QWEFANDDFVR + HLMKNIHRRKPVHSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANDDFVRSKPHLMKNIHRRKPVHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           S PLTEVER    D+IE LK DKEQLLLEL+KHEQEYQGV LQMQNLKDRFQCVQQ MQ 
Sbjct: 121 S-PLTEVERNGLNDDIERLKLDKEQLLLELQKHEQEYQGVGLQMQNLKDRFQCVQQEMQS 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FISL+AR L KPGL LDLLPQLET +RKRRLPRVSYNN+ED LEDNQMGTTQTI R++  
Sbjct: 181 FISLMARILQKPGLHLDLLPQLETPERKRRLPRVSYNNNEDKLEDNQMGTTQTIGRDDMG 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFD I K+EQFEL+ETSLTFWEGII SYGQT+SPLDSSSNLELGG VSHASSPA +CRQ
Sbjct: 241 CSFDSIFKKEQFELIETSLTFWEGIILSYGQTVSPLDSSSNLELGGCVSHASSPATSCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEE RCKSPGIDMNLEP+ TVAP+S+ASKDQ AGV APVPTG NDVFWQQFLTENPG+S
Sbjct: 301 VSEEFRCKSPGIDMNLEPVPTVAPDSLASKDQEAGVNAPVPTGANDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKF 411
           DPQEVQSARKDSDVI +ENR SD G FWWNTRSVNNVVEQIG+L PAEKF
Sbjct: 361 DPQEVQSARKDSDVINDENRQSDHGKFWWNTRSVNNVVEQIGHLKPAEKF 409

BLAST of Carg19899 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.5e-92
Identity = 193/415 (46.51%), Postives = 265/415 (63.86%), Query Frame = 0

Query: 1   MDE-AQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFK 60
           MDE   G   +SLPPFL KTY+MVDD S++SIVSWS S+KSF+VW P EFS  LLP+FFK
Sbjct: 1   MDENNHGVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFK 60

Query: 61  HSNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQG 120
           H+NFSSFIRQLNTYGF+K DP+QWEFANDDFVRG+ HLMKNIHRRKPVHSHSL  P+ Q 
Sbjct: 61  HNNFSSFIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSL--PNLQA 120

Query: 121 VSPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQ 180
              PLT+ ER    + IE L ++KE LL EL K ++E +   +Q++ LK+R Q +++  +
Sbjct: 121 QLNPLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQK 180

Query: 181 LFISLIARFLHKPGLRLDLLPQL-ETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISREN 240
             +S +++ L KPGL L+L P + ET++RKRR PR+ +   E  LE+N+   T  + RE 
Sbjct: 181 TMVSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENK---TCVVVREE 240

Query: 241 RDCSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTC 300
              S     +E Q E +E+S+  WE ++    +++    S   L++  S +   SP ++C
Sbjct: 241 GSTSPSSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSC 300

Query: 301 RQVSEELRCKSPG----IDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLT 360
            Q+S + R KSP     IDMN EP  +    +VA+         P   G ND FWQQF +
Sbjct: 301 IQLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVAAP------PPPPVAGANDGFWQQFFS 360

Query: 361 ENPGSSDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEK 410
           ENPGS++ +EVQ  RKD     ++         WWN+R+VN + EQ+G+L  +E+
Sbjct: 361 ENPGSTEQREVQLERKDD----KDKAGVRTEKCWWNSRNVNAITEQLGHLTSSER 400

BLAST of Carg19899 vs. ExPASy Swiss-Prot
Match: Q9FK72 (Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 9.4e-82
Identity = 185/401 (46.13%), Postives = 238/401 (59.35%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDE  G G +SLPPFL KTY+MVDD S++S+V+WS ++KSF+V  P EFS  LLP+FFKH
Sbjct: 1   MDENNG-GSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
            NFSSFIRQLNTYGF+KVDP++WEF NDDFVRG  +LMKNIHRRKPVHSHSL N   Q  
Sbjct: 61  KNFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN- 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
             PLTE ER+S ED IE LK +KE LL EL+  EQE +   LQ+  LKDR Q ++Q  + 
Sbjct: 121 --PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQHMEQHQKS 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
            ++ +++ L KPGL L+    LE  +R++R             ++N +  + +       
Sbjct: 181 IVAYVSQVLGKPGLSLN----LENHERRKR-----------RFQENSLPPSSS------- 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
                    EQ E +E+SLTFWE ++           S S  + G   S     A     
Sbjct: 241 -------HIEQVEKLESSLTFWENLV-----------SESCEKSGLQSSSMDHDAAESSL 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVP-TGVNDVFWQQFLTENPGS 360
              + R KS  IDMN EP  TV               AP P TGVND FW+Q LTENPGS
Sbjct: 301 SIGDTRPKSSKIDMNSEPPVTVT--------------APAPKTGVNDDFWEQCLTENPGS 343

Query: 361 SDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQ 401
           ++ QEVQS R+D       N+  +Q  +WWN+ +VNN+ E+
Sbjct: 361 TEQQEVQSERRDVGNDNNGNKIGNQRTYWWNSGNVNNITEK 343

BLAST of Carg19899 vs. ExPASy Swiss-Prot
Match: Q94J16 (Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4B PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.6e-73
Identity = 175/443 (39.50%), Postives = 244/443 (55.08%), Query Frame = 0

Query: 6   GSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSS 65
           G G  SLPPFL KTY+MVDDPST+++V W+ +  SFVV    EF   LLPK+FKH+NFSS
Sbjct: 4   GGGGGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQPEFCRDLLPKYFKHNNFSS 63

Query: 66  FIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSPPLT 125
           F+RQLNTYGF+KVDP+QWEFAN+DF++G++H +KNIHRRKP+ SHS    H QG   PLT
Sbjct: 64  FVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRRKPIFSHS---SHSQGAG-PLT 123

Query: 126 EVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQLFISLI 185
           + ERK +E+ IE LK D   L  EL+ +  +   +  +MQ L+++   V+   +  IS +
Sbjct: 124 DNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQALEEKLFVVEDQQRSLISYV 183

Query: 186 ARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQM-------GTTQTISREN 245
              +  PG     + Q +   +KRRLP     + + N ++NQ+          QT  RE+
Sbjct: 184 REIVKAPGFLSSFVQQQDHHRKKRRLPIPISFHEDANTQENQIMPCDLTNSPAQTFYRES 243

Query: 246 RDCSFDPILKEEQF-----ELVETSLTFWEGI-----------IHSYGQT----ISPL-- 305
            D     +   E F     E     +++ +G+           +HS G++     SP   
Sbjct: 244 FDKMESSLNSLENFLREASEEFGNDISYDDGVPGPSSTVVLTELHSPGESDPRVSSPPTR 303

Query: 306 ---------DSSSNLELGGSVSHASSPAVTCRQVSEELRCKSPGIDMNLEPMATVAPESV 365
                    DS S+ ++  S S A SP +       + R K   ID+N EP  T   E+ 
Sbjct: 304 MRTSSAGAGDSHSSRDVAESTSCAESPPIPQMHSRVDTRAKVSEIDVNSEPAVT---ETG 363

Query: 366 ASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSSDP-QEVQSARKDSDVIIEENRPSDQGN 410
            S+DQ A     V  G ND FWQQFLTE PGSSD  QE QS R+D    ++E +  D+ +
Sbjct: 364 PSRDQPAEEPPAVTPGANDGFWQQFLTEQPGSSDAHQEAQSERRDGGNKVDEMKSGDRQH 423

BLAST of Carg19899 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 8.6e-59
Identity = 166/462 (35.93%), Postives = 242/462 (52.38%), Query Frame = 0

Query: 6   GSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSS 65
           G G    PPFL+KTY+MV+D +TN +VSW     SFVVW PL+FS  LLPK+FKH+NFSS
Sbjct: 12  GGGGGGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNNFSS 71

Query: 66  FIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSPPLT 125
           FIRQLNTYGF+K+DP++WEFAN+DF+RG  HL+KNIHRRKPVHSHSLQN     ++ PL 
Sbjct: 72  FIRQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHSLQNQ----INGPLA 131

Query: 126 EVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQLFISLI 185
           E ER+  E+ I  LK +K  L+ +L++  Q+   +  QMQ ++ R   ++Q  +  ++ +
Sbjct: 132 ESERRELEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVAMEQRQKNIVASL 191

Query: 186 ARFLHKPGLRL-DLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRDCSFD 245
              L + G  +   L + +   +KRR+P++        ++D   G  Q +  + +    D
Sbjct: 192 CEMLQRRGGAVSSSLLESDHFSKKRRVPKMDL-----FVDDCAAGEEQKVF-QFQGIGTD 251

Query: 246 --------PILKEEQFELVETSLTFWEGII---------------HSYGQT--------- 305
                   P+   E F+ VE SL   E +                H +G T         
Sbjct: 252 APAMPPVLPVTNGEAFDRVELSLVSLEKLFQRANDACTAAEEMYSHGHGGTEPSTAICPE 311

Query: 306 ---ISPLDSSSNLELGGSVSHASSPAV--TCRQVSEELRCKSPGIDMNLE-PMATVAPE- 365
               +P+++  +L+L  S+ H SSP        +S EL  +SPG   + E PMA +  + 
Sbjct: 312 EMNTAPMETGIDLQLPASL-HPSSPNTGNAHLHLSTEL-TESPGFVQSPELPMAEIREDI 371

Query: 366 SVASKDQAAGVKAPVPTG-----------------VNDVFWQQFLTENPGSS-DPQEVQS 410
            V      A V + + +                   NDVFW++FLTE P S  D  E Q 
Sbjct: 372 HVTRYPTQADVNSEIASSTDTSQDGTSETEASHGPTNDVFWERFLTETPRSCLDESERQE 431

BLAST of Carg19899 vs. ExPASy Swiss-Prot
Match: Q84T61 (Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA1 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.3e-51
Identity = 140/362 (38.67%), Postives = 199/362 (54.97%), Query Frame = 0

Query: 10  TSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIRQ 69
           T+ PPFL+KTY+MVDDP+T+++VSW   + SFVVW   EF+  LLPK+FKHSNFSSF+RQ
Sbjct: 33  TAPPPFLMKTYEMVDDPATDAVVSWGPGNNSFVVWNTPEFARDLLPKYFKHSNFSSFVRQ 92

Query: 70  LNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVH-SHSLQNPHGQGVS-PPLTEV 129
           LNTYGF+KVDPD+WEFAN+ F+RG++HL+K I+RRKP H ++ +Q P       P   EV
Sbjct: 93  LNTYGFRKVDPDRWEFANEGFLRGQKHLLKTINRRKPTHGNNQVQQPQLPAAPVPACVEV 152

Query: 130 ERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQLFISLIAR 189
            +   E+ IE LKRDK  L+ EL +  Q+ Q    Q+Q L  R Q ++Q  Q  +S +A+
Sbjct: 153 GKFGMEEEIEMLKRDKNVLMQELVRLRQQQQTTDHQLQTLGKRLQGMEQRQQQMMSFLAK 212

Query: 190 FLHKPGLRLDLLPQLE-------TSDRKRRLPRVSYN-NSEDNLEDNQMGTTQTISRENR 249
            +H PG     + Q E        S++KRRLP+   + +SE    D Q+   Q +  E  
Sbjct: 213 AMHSPGFLAQFVQQNENSRRRIVASNKKRRLPKQDGSLDSESASLDGQIVKYQPMINEAA 272

Query: 250 DCSFDPILK---EEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGG---------- 309
                 ILK     +FE +  S  F   ++ +Y      LDSSS+    G          
Sbjct: 273 KAMLRKILKLDSSHRFESMGNSDNF---LLENYMPNGQGLDSSSSTRNSGVTLAEVPANS 332

Query: 310 ---SVSHASSPAVTCRQVSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTG 346
               V+ +S  +  C   + +++C  P +  N  P       +V S  +A    AP PT 
Sbjct: 333 GLPYVATSSGLSAICSTSTPQIQC--PVVLDNGIPKEVPNMSAVPSVPKAV---APGPTD 386

BLAST of Carg19899 vs. ExPASy TrEMBL
Match: A0A6J1EI67 (heat stress transcription factor A-4c-like OS=Cucurbita moschata OX=3662 GN=LOC111434310 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 6.5e-235
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 412
           DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411

BLAST of Carg19899 vs. ExPASy TrEMBL
Match: A0A6J1KEK1 (heat stress transcription factor A-4a-like OS=Cucurbita maxima OX=3661 GN=LOC111495117 PE=3 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.2e-214
Identity = 376/388 (96.91%), Postives = 383/388 (98.71%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKV P+QWEFANDDFVRG+QHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVHPEQWEFANDDFVRGKQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFED+IETLKRDKEQ+LLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDDIETLKRDKEQILLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FISLIAR LHKPG RLDLLPQLETSDRKRRLPRVSYNNSEDN+EDNQMGTTQTISREN D
Sbjct: 181 FISLIARLLHKPGRRLDLLPQLETSDRKRRLPRVSYNNSEDNIEDNQMGTTQTISRENMD 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFELVETSLTFWEGI+HSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIVHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPES+ASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESIASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFW 389
           DPQEVQSARKDSDVIIEENR SDQGNFW
Sbjct: 361 DPQEVQSARKDSDVIIEENRRSDQGNFW 388

BLAST of Carg19899 vs. ExPASy TrEMBL
Match: A0A0A0LV22 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G467710 PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.4e-197
Identity = 349/410 (85.12%), Postives = 377/410 (91.95%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW PLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGF+KVDP+QWEFAN+DFVRG+ HLMKNIHRRKP+HSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGKPHLMKNIHRRKPIHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           S PLTEVER SF+D+IE LK DKEQLLLEL+K+EQEYQGV LQ+QNLKDRFQ VQQ MQL
Sbjct: 121 S-PLTEVERNSFKDDIERLKLDKEQLLLELQKYEQEYQGVGLQIQNLKDRFQRVQQEMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FISL+AR L KPGL LDLLPQLET +RKRRLPRVSYN SED+LEDN +GTTQTI R++  
Sbjct: 181 FISLMARLLQKPGLHLDLLPQLETPERKRRLPRVSYNISEDSLEDNHLGTTQTIGRDDMG 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPIL++EQ EL+ETSLTFWEGIIHSY +T+SPLDSSSNLEL GSVSHASSPA++CR 
Sbjct: 241 CSFDPILEKEQLELLETSLTFWEGIIHSYDETVSPLDSSSNLELVGSVSHASSPAISCRL 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           V EE RCKSPGIDMNLEPMATVAP+SVASKDQAAGV AP+PTG NDVFWQQFLTENPG+S
Sbjct: 301 VREEFRCKSPGIDMNLEPMATVAPDSVASKDQAAGVNAPLPTGFNDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKF 411
           DPQEVQSARKDSDVI EENR SD G FWWNTRSVNNVVEQIG+L PAEKF
Sbjct: 361 DPQEVQSARKDSDVINEENRQSDHGKFWWNTRSVNNVVEQIGHLKPAEKF 409

BLAST of Carg19899 vs. ExPASy TrEMBL
Match: A0A1S4DVL4 (heat stress transcription factor A-4c OS=Cucumis melo OX=3656 GN=LOC103487107 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 3.1e-197
Identity = 348/410 (84.88%), Postives = 375/410 (91.46%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW PLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGF+KVDP+QWEFAN+DFVRG+ HLMKNIHRRKP+HSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGKPHLMKNIHRRKPIHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           S PLTEVER SF+DNIE LK DKEQLLLEL+K+EQEYQGV LQMQNLKDRFQ VQQGMQL
Sbjct: 121 S-PLTEVERNSFKDNIERLKLDKEQLLLELQKYEQEYQGVGLQMQNLKDRFQRVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
           FI L+AR   KPGLRLDLLPQLET +RKRRLPR SYN SED+LED+Q+GTTQ I RE+  
Sbjct: 181 FIGLMARLFQKPGLRLDLLPQLETPERKRRLPRASYNISEDSLEDDQLGTTQAIGREDLS 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPIL++EQ EL+ETSLTFWEGIIHSY QT+ PLDSSSNLEL GSVSHASSPA++CR 
Sbjct: 241 CSFDPILEKEQLELLETSLTFWEGIIHSYDQTVIPLDSSSNLELVGSVSHASSPAISCRL 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           V EE RCKSPGIDMNLEPMATVAP+SVASKDQAAGV AP+PTG NDVFWQQFLTENPG+S
Sbjct: 301 VREEFRCKSPGIDMNLEPMATVAPDSVASKDQAAGVNAPLPTGFNDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKF 411
           DPQEVQSARKDSDVI EEN+ SD  NFWWNTRSVNN+VEQIG+L PAEKF
Sbjct: 361 DPQEVQSARKDSDVINEENKQSDHENFWWNTRSVNNIVEQIGHLKPAEKF 409

BLAST of Carg19899 vs. ExPASy TrEMBL
Match: A0A6J1CE08 (heat stress transcription factor A-4c-like OS=Momordica charantia OX=3673 GN=LOC111010635 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.9e-182
Identity = 329/413 (79.66%), Postives = 367/413 (88.86%), Query Frame = 0

Query: 1   MDEAQ-GSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFK 60
           MDEAQ G GL+SLPPFLVKTYDMVDDPST+SIVSW+ S+KSFVV  PLEFSSVLLPKFFK
Sbjct: 1   MDEAQGGGGLSSLPPFLVKTYDMVDDPSTDSIVSWTPSNKSFVVRNPLEFSSVLLPKFFK 60

Query: 61  HSNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQG 120
           HSNFSSFIRQLNTYGF+KVDP+QWEFAN+DFVRG+ +LMKNIHRRKPVHSHSLQN HGQG
Sbjct: 61  HSNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGQPYLMKNIHRRKPVHSHSLQNIHGQG 120

Query: 121 VSPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQ 180
           +S PLTEVERK  + +IE LK+DKEQLLLELR+HEQE+QGV LQMQNLKDRF+ +QQ MQ
Sbjct: 121 ISSPLTEVERKGLKGDIERLKQDKEQLLLELRRHEQEHQGVGLQMQNLKDRFEHMQQQMQ 180

Query: 181 LFISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQM-GTTQTISREN 240
            FISL+      PGLRLDLLP+LET +RKRRLPR++YNN+ED LED+QM GTTQ+++REN
Sbjct: 181 TFISLVV-----PGLRLDLLPRLETPERKRRLPRIAYNNNEDKLEDDQMGGTTQSVAREN 240

Query: 241 RDCSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTC 300
            DCSFDPILK EQFEL ETSL FWEGIIHS+GQ +SPLDSSS LEL  S SHASSPA++ 
Sbjct: 241 MDCSFDPILKREQFELFETSLIFWEGIIHSFGQKVSPLDSSSYLELDESTSHASSPAMSH 300

Query: 301 RQVSEELRCKSPGIDMNLE-PMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENP 360
           RQVSEE RCKSPGIDMNLE P+ATVAPESVAS+DQAAGV APVPTGVND FW+QFLTENP
Sbjct: 301 RQVSEEFRCKSPGIDMNLEPPVATVAPESVASRDQAAGVNAPVPTGVNDGFWEQFLTENP 360

Query: 361 GSSDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKF 411
           GSSDPQEVQSARKDS+V++EE R  D G FWWN RSVNNVVEQIG+L PAEKF
Sbjct: 361 GSSDPQEVQSARKDSNVVVEEKRQRDHGKFWWNVRSVNNVVEQIGHLTPAEKF 408

BLAST of Carg19899 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-93
Identity = 193/415 (46.51%), Postives = 265/415 (63.86%), Query Frame = 0

Query: 1   MDE-AQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFK 60
           MDE   G   +SLPPFL KTY+MVDD S++SIVSWS S+KSF+VW P EFS  LLP+FFK
Sbjct: 1   MDENNHGVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFK 60

Query: 61  HSNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQG 120
           H+NFSSFIRQLNTYGF+K DP+QWEFANDDFVRG+ HLMKNIHRRKPVHSHSL  P+ Q 
Sbjct: 61  HNNFSSFIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSL--PNLQA 120

Query: 121 VSPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQ 180
              PLT+ ER    + IE L ++KE LL EL K ++E +   +Q++ LK+R Q +++  +
Sbjct: 121 QLNPLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQK 180

Query: 181 LFISLIARFLHKPGLRLDLLPQL-ETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISREN 240
             +S +++ L KPGL L+L P + ET++RKRR PR+ +   E  LE+N+   T  + RE 
Sbjct: 181 TMVSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENK---TCVVVREE 240

Query: 241 RDCSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTC 300
              S     +E Q E +E+S+  WE ++    +++    S   L++  S +   SP ++C
Sbjct: 241 GSTSPSSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSC 300

Query: 301 RQVSEELRCKSPG----IDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLT 360
            Q+S + R KSP     IDMN EP  +    +VA+         P   G ND FWQQF +
Sbjct: 301 IQLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVAAP------PPPPVAGANDGFWQQFFS 360

Query: 361 ENPGSSDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEK 410
           ENPGS++ +EVQ  RKD     ++         WWN+R+VN + EQ+G+L  +E+
Sbjct: 361 ENPGSTEQREVQLERKDD----KDKAGVRTEKCWWNSRNVNAITEQLGHLTSSER 400

BLAST of Carg19899 vs. TAIR 10
Match: AT5G45710.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 305.4 bits (781), Expect = 6.7e-83
Identity = 185/401 (46.13%), Postives = 238/401 (59.35%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDE  G G +SLPPFL KTY+MVDD S++S+V+WS ++KSF+V  P EFS  LLP+FFKH
Sbjct: 1   MDENNG-GSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
            NFSSFIRQLNTYGF+KVDP++WEF NDDFVRG  +LMKNIHRRKPVHSHSL N   Q  
Sbjct: 61  KNFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN- 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180
             PLTE ER+S ED IE LK +KE LL EL+  EQE +   LQ+  LKDR Q ++Q  + 
Sbjct: 121 --PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQHMEQHQKS 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240
            ++ +++ L KPGL L+    LE  +R++R             ++N +  + +       
Sbjct: 181 IVAYVSQVLGKPGLSLN----LENHERRKR-----------RFQENSLPPSSS------- 240

Query: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
                    EQ E +E+SLTFWE ++           S S  + G   S     A     
Sbjct: 241 -------HIEQVEKLESSLTFWENLV-----------SESCEKSGLQSSSMDHDAAESSL 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVP-TGVNDVFWQQFLTENPGS 360
              + R KS  IDMN EP  TV               AP P TGVND FW+Q LTENPGS
Sbjct: 301 SIGDTRPKSSKIDMNSEPPVTVT--------------APAPKTGVNDDFWEQCLTENPGS 343

Query: 361 SDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQ 401
           ++ QEVQS R+D       N+  +Q  +WWN+ +VNN+ E+
Sbjct: 361 TEQQEVQSERRDVGNDNNGNKIGNQRTYWWNSGNVNNITEK 343

BLAST of Carg19899 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 197.6 bits (501), Expect = 2.0e-50
Identity = 110/227 (48.46%), Postives = 144/227 (63.44%), Query Frame = 0

Query: 9   LTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIR 68
           + S+PPFL KTYDMVDDP TN +VSWSS + SFVVW   EFS VLLPK+FKH+NFSSF+R
Sbjct: 22  VNSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVR 81

Query: 69  QLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSP----PL 128
           QLNTYGF+KVDPD+WEFAN+ F+RG + L+K+I RRKP  SH  QN     V        
Sbjct: 82  QLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKP--SHVQQNQQQTQVQSSSVGAC 141

Query: 129 TEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQLFISL 188
            EV +   E+ +E LKRDK  L+ EL +  Q+ Q    Q+QN+  + Q ++Q  Q  +S 
Sbjct: 142 VEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSF 201

Query: 189 IARFLHKPGLRLDLLP--------QLETSDRKRRLPRVSYNNSEDNL 224
           +A+ +  PG    L+         Q+  S++KRRLP     N  DN+
Sbjct: 202 LAKAVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNV 246

BLAST of Carg19899 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 197.6 bits (501), Expect = 2.0e-50
Identity = 110/227 (48.46%), Postives = 144/227 (63.44%), Query Frame = 0

Query: 9   LTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIR 68
           + S+PPFL KTYDMVDDP TN +VSWSS + SFVVW   EFS VLLPK+FKH+NFSSF+R
Sbjct: 22  VNSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVR 81

Query: 69  QLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSP----PL 128
           QLNTYGF+KVDPD+WEFAN+ F+RG + L+K+I RRKP  SH  QN     V        
Sbjct: 82  QLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKP--SHVQQNQQQTQVQSSSVGAC 141

Query: 129 TEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQLFISL 188
            EV +   E+ +E LKRDK  L+ EL +  Q+ Q    Q+QN+  + Q ++Q  Q  +S 
Sbjct: 142 VEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSF 201

Query: 189 IARFLHKPGLRLDLLP--------QLETSDRKRRLPRVSYNNSEDNL 224
           +A+ +  PG    L+         Q+  S++KRRLP     N  DN+
Sbjct: 202 LAKAVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNV 246

BLAST of Carg19899 vs. TAIR 10
Match: AT4G13980.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 196.1 bits (497), Expect = 5.7e-50
Identity = 147/465 (31.61%), Postives = 228/465 (49.03%), Query Frame = 0

Query: 6   GSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSS 65
           G G     PFLVKTY+MVDD ST+ IVSWS+++ SF+VW   EFS +LLP +FKH+NFSS
Sbjct: 15  GEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFKHNNFSS 74

Query: 66  FIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSPPL- 125
           FIRQLNTYGF+K+DP++WEF NDDF++ ++HL+KNIHRRKP+HSHS          PP  
Sbjct: 75  FIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHS---------HPPAS 134

Query: 126 -TEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQLFIS 185
            T+ ER   ++ ++ L R+K  +  +L K +Q+      Q + + +    ++   +  ++
Sbjct: 135 STDQERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLN 194

Query: 186 LIARFLHKP------GLRLDLLPQLETSDRKRRLPRVSYNN--SEDNLEDNQMGTTQTIS 245
            +   +  P      G +++ L  +   ++KRRLP V  +   SED+  DN  G+++  S
Sbjct: 195 FLETAIRNPTFVKNFGKKVEQL-DISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRES 254

Query: 246 RENRDCSFDPILK------EEQFELV-----------------------ETSLTFWEGI- 305
                 +F   L+      +    +V                        T+LT  EG+ 
Sbjct: 255 GNIFHQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLP 314

Query: 306 -------------------------IHSYGQTIS---PLDSSSNLELGGSVSHASSPAVT 365
                                    + +  Q ++     D S +  L  +++ A  P  T
Sbjct: 315 FAPEALELADTGTCPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAPLPDKT 374

Query: 366 CRQVSEELRCKSPGIDMNLEPMATVAPESVASKDQAA----GVKAPVPTGVNDVFWQQFL 398
             Q++ +   KS   ++N   + T A E    + + A       A  P  VNDVFW+QFL
Sbjct: 375 ASQIA-KTTLKSQ--ELNFNSIETSASEKNRGRQEIAVGGSQANAAPPARVNDVFWEQFL 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022927503.11.3e-234100.00heat stress transcription factor A-4c-like [Cucurbita moschata] >XP_022927504.1 ... [more]
XP_023519250.16.2e-23298.54heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
KAG6583940.16.2e-224100.00Heat stress transcription factor A-4a, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023000762.14.5e-21496.91heat stress transcription factor A-4a-like [Cucurbita maxima] >XP_023000763.1 he... [more]
XP_038895068.11.5e-19885.61heat stress transcription factor A-4a [Benincasa hispida] >XP_038895070.1 heat s... [more]
Match NameE-valueIdentityDescription
O494031.5e-9246.51Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ... [more]
Q9FK729.4e-8246.13Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C ... [more]
Q94J163.6e-7339.50Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q93VB58.6e-5935.93Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q84T611.3e-5138.67Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1EI676.5e-235100.00heat stress transcription factor A-4c-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KEK12.2e-21496.91heat stress transcription factor A-4a-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0LV222.4e-19785.12HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G467710 ... [more]
A0A1S4DVL43.1e-19784.88heat stress transcription factor A-4c OS=Cucumis melo OX=3656 GN=LOC103487107 PE... [more]
A0A6J1CE084.9e-18279.66heat stress transcription factor A-4c-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G18880.11.1e-9346.51heat shock transcription factor A4A [more]
AT5G45710.16.7e-8346.13winged-helix DNA-binding transcription factor family protein [more]
AT5G16820.12.0e-5048.46heat shock factor 3 [more]
AT5G16820.22.0e-5048.46heat shock factor 3 [more]
AT4G13980.15.7e-5031.61winged-helix DNA-binding transcription factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 129..170
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 355..382
NoneNo IPR availablePANTHERPTHR10015:SF161HEAT STRESS TRANSCRIPTION FACTOR A-4Ccoord: 1..391
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 66..78
score: 78.68
coord: 15..38
score: 46.07
coord: 53..65
score: 72.09
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 11..104
e-value: 2.5E-57
score: 206.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 15..104
e-value: 3.1E-30
score: 104.6
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 54..78
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 9..105
e-value: 9.3E-36
score: 124.4
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 1..391
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 11..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19899-RACarg19899-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding