Carg19667 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19667
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontranscription factor MYB44-like
LocationCarg_Chr16: 1876059 .. 1877309 (-)
RNA-Seq ExpressionCarg19667
SyntenyCarg19667
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTCTAGGAAGGGGAAAATCGATCGGGTCAAGGGCCCATGGAGCCCCGAGGAAGACGAGATGCTGCGAATGCTAGTGCAGAGCCAAGGCGCTCGGAACTGGTCCATTATCAGTCAATCGATTCACGGTCGGTCAGGAAAATCGTGCCGTCTGCGGTGGTTTAATCAGCTCTGTCCTGGTGTAGAGCGTCGCGCTTTCACGGCGGAGGAGGACGAGGTCATCACCGAAGCCCACGCCAAATACGGAAACAAGTGGGCGACGATCGCGAGGCTTCTCAATGGACGAACAGACAATGCGATTAAGAATCACTGGAATTCGACATTGAAGCGCAAGTTCTGTTCGATTGCGGAGAAGAAAACCGATTGTGCAAGTCAAGGATCAACGATTTCGGTATCGAATCAGTGTTTGAGTCCGTCTGGATCTGAGACTAGCCATTCGATTCGGAGTAGCGATATGGAGCCGTCAACTCAATTAACTCTGTCGCTTCCAGGATCAGAGCTGTGGCCAGTAACGCAGCAGCAGAGTATTTCATCATCGCCGGTGAAAAGTGAGGAGAAACAGGAAGGGAAACGAACGTGTTTCCGCGAGGAATTGTTGTGTGTGATGCAGAAGATGATTAAAGAAGAGGTGAGAAATTATGTGGCGCAATATGAAGAAGTTAGAAACGGCAAAAAAGGAATCGATGAATGAAGAAGAAGAAGAACAGATGAATTAGGGCCGAACAAATGAAAGAAGATCAAAAGGTACTTTGATTTCGTGTTTTTGTTACTTAGTTATTCAAATTGGAGCCAGTAGTCCTTCGTTAGAGATTCTTCATTTTCCATTATCTCAGAAAATTTTCCTCCCTTTTCTCACTTTTTTCTTTGTACATCTGTATTTTGAATTCGTTTCAGTTCAATCATTGGGAGGTAGGATTGAATAATCAACTTTTGTTTGTACCAGTGAATGTGATTTTATTTACTAAATTAGAATCAAAATTTGTTGATGGAGATCTGTATGTAGTAGAGATATCGAACATTTTTCATTCCAATTACTAACCTTAAGCTTACCTCGAGAGGTACGACCTTGAGTAAAAGCTCCAAGACAGACGAGGAACTGCTTGAGCTTAAGCTTATACTTTGCATGCAGGAGGGAACCTCAATCCTGAAAGCTTATGGTGCTATCACTTCACATTTTACCTGCAGGGACCCAGATATACATGAAAGCTTACGGTGGTGCTTCCACCGTCGCCCATGGGTGAGTCGGTGA

mRNA sequence

ATGGGGTCTAGGAAGGGGAAAATCGATCGGGTCAAGGGCCCATGGAGCCCCGAGGAAGACGAGATGCTGCGAATGCTAGTGCAGAGCCAAGGCGCTCGGAACTGGTCCATTATCAGTCAATCGATTCACGGTCGGTCAGGAAAATCGTGCCGTCTGCGGTGGTTTAATCAGCTCTGTCCTGGTGTAGAGCGTCGCGCTTTCACGGCGGAGGAGGACGAGGTCATCACCGAAGCCCACGCCAAATACGGAAACAAGTGGGCGACGATCGCGAGGCTTCTCAATGGACGAACAGACAATGCGATTAAGAATCACTGGAATTCGACATTGAAGCGCAAGTTCTGTTCGATTGCGGAGAAGAAAACCGATTGTGCAAGTCAAGGATCAACGATTTCGGTATCGAATCAGTGTTTGAGTCCGTCTGGATCTGAGACTAGCCATTCGATTCGGAGTAGCGATATGGAGCCGTCAACTCAATTAACTCTGTCGCTTCCAGGATCAGAGCTGTGGCCAGTAACGCAGCAGCAGAGTATTTCATCATCGCCGGTGAAAAGTGAGGAGAAACAGGAAGGGAAACGAACGTGTTTCCGCGAGGAATTGTTGTGTGTGATGCAGAAGATGATTAAAGAAGAGGAGGGAACCTCAATCCTGAAAGCTTATGGTGCTATCACTTCACATTTTACCTGCAGGGACCCAGATATACATGAAAGCTTACGGTGGTGCTTCCACCGTCGCCCATGGGTGAGTCGGTGA

Coding sequence (CDS)

ATGGGGTCTAGGAAGGGGAAAATCGATCGGGTCAAGGGCCCATGGAGCCCCGAGGAAGACGAGATGCTGCGAATGCTAGTGCAGAGCCAAGGCGCTCGGAACTGGTCCATTATCAGTCAATCGATTCACGGTCGGTCAGGAAAATCGTGCCGTCTGCGGTGGTTTAATCAGCTCTGTCCTGGTGTAGAGCGTCGCGCTTTCACGGCGGAGGAGGACGAGGTCATCACCGAAGCCCACGCCAAATACGGAAACAAGTGGGCGACGATCGCGAGGCTTCTCAATGGACGAACAGACAATGCGATTAAGAATCACTGGAATTCGACATTGAAGCGCAAGTTCTGTTCGATTGCGGAGAAGAAAACCGATTGTGCAAGTCAAGGATCAACGATTTCGGTATCGAATCAGTGTTTGAGTCCGTCTGGATCTGAGACTAGCCATTCGATTCGGAGTAGCGATATGGAGCCGTCAACTCAATTAACTCTGTCGCTTCCAGGATCAGAGCTGTGGCCAGTAACGCAGCAGCAGAGTATTTCATCATCGCCGGTGAAAAGTGAGGAGAAACAGGAAGGGAAACGAACGTGTTTCCGCGAGGAATTGTTGTGTGTGATGCAGAAGATGATTAAAGAAGAGGAGGGAACCTCAATCCTGAAAGCTTATGGTGCTATCACTTCACATTTTACCTGCAGGGACCCAGATATACATGAAAGCTTACGGTGGTGCTTCCACCGTCGCCCATGGGTGAGTCGGTGA

Protein sequence

MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKKTDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSSPVKSEEKQEGKRTCFREELLCVMQKMIKEEEGTSILKAYGAITSHFTCRDPDIHESLRWCFHRRPWVSR
Homology
BLAST of Carg19667 vs. NCBI nr
Match: KAG6576937.1 (Transcription factor MYB44, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014962.1 Transcription factor MYB44, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 509.6 bits (1311), Expect = 1.5e-140
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60
           MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP
Sbjct: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60

Query: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120
           GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK
Sbjct: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120

Query: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180
           TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS
Sbjct: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180

Query: 181 PVKSEEKQEGKRTCFREELLCVMQKMIKEEEGTSILKAYGAITSHFTCRDPDIHESLRWC 240
           PVKSEEKQEGKRTCFREELLCVMQKMIKEEEGTSILKAYGAITSHFTCRDPDIHESLRWC
Sbjct: 181 PVKSEEKQEGKRTCFREELLCVMQKMIKEEEGTSILKAYGAITSHFTCRDPDIHESLRWC 240

Query: 241 FHRRPWVSR 250
           FHRRPWVSR
Sbjct: 241 FHRRPWVSR 249

BLAST of Carg19667 vs. NCBI nr
Match: XP_022922824.1 (transcription factor MYB44-like [Cucurbita moschata])

HSP 1 Score: 413.7 bits (1062), Expect = 1.1e-111
Identity = 206/210 (98.10%), Postives = 208/210 (99.05%), Query Frame = 0

Query: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60
           MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP
Sbjct: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60

Query: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120
           GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK
Sbjct: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120

Query: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180
           TDCASQGSTIS+SNQCLSPSGSETS SIRSSDMEPSTQLTLSLPGSELWPVTQQQ ISSS
Sbjct: 121 TDCASQGSTISISNQCLSPSGSETSDSIRSSDMEPSTQLTLSLPGSELWPVTQQQCISSS 180

Query: 181 PVKSEEKQEGKRTCFREELLCVMQKMIKEE 211
           PVKSEEKQ+GKRTCFREELLCVMQKMIKEE
Sbjct: 181 PVKSEEKQKGKRTCFREELLCVMQKMIKEE 210

BLAST of Carg19667 vs. NCBI nr
Match: XP_023552561.1 (transcription factor MYB73-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.1 bits (1045), Expect = 1.1e-109
Identity = 203/210 (96.67%), Postives = 206/210 (98.10%), Query Frame = 0

Query: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60
           MGS+KGKIDRVKGPW PEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP
Sbjct: 1   MGSKKGKIDRVKGPWIPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60

Query: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120
           GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK
Sbjct: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120

Query: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180
           TDC SQGSTISVSNQCLSPSGSETS SIRSSDMEPSTQLTLSLPG+ELWPVTQQQSISSS
Sbjct: 121 TDCVSQGSTISVSNQCLSPSGSETSDSIRSSDMEPSTQLTLSLPGTELWPVTQQQSISSS 180

Query: 181 PVKSEEKQEGKRTCFREELLCVMQKMIKEE 211
           PVKSEEKQ+GKRTCF EELLCVMQKMIKEE
Sbjct: 181 PVKSEEKQKGKRTCFGEELLCVMQKMIKEE 210

BLAST of Carg19667 vs. NCBI nr
Match: XP_022985148.1 (transcription factor MYB44-like [Cucurbita maxima])

HSP 1 Score: 403.7 bits (1036), Expect = 1.2e-108
Identity = 203/210 (96.67%), Postives = 206/210 (98.10%), Query Frame = 0

Query: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60
           MGSRKG IDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP
Sbjct: 1   MGSRKGNIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60

Query: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120
           GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK
Sbjct: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120

Query: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180
           TDCASQGSTISVSNQCLSPSGSETS SIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS
Sbjct: 121 TDCASQGSTISVSNQCLSPSGSETSDSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180

Query: 181 PVKSEEKQEGKRTCFREELLCVMQKMIKEE 211
           P +SEEKQ+GKRTCF EELLCVMQKM+KEE
Sbjct: 181 P-RSEEKQKGKRTCFGEELLCVMQKMVKEE 209

BLAST of Carg19667 vs. NCBI nr
Match: XP_038900077.1 (transcription factor MYB73-like [Benincasa hispida])

HSP 1 Score: 322.8 bits (826), Expect = 2.6e-84
Identity = 165/211 (78.20%), Postives = 183/211 (86.73%), Query Frame = 0

Query: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60
           M S +  IDRVKGPWSPEEDE+LRMLV++QGARNWS+ISQSI+GRSGKSCRLRWFNQLCP
Sbjct: 1   MASMQADIDRVKGPWSPEEDELLRMLVKNQGARNWSLISQSINGRSGKSCRLRWFNQLCP 60

Query: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120
           GVER AFTAEEDE+I EAH KYGNKWATIARLLNGRTDNAIKNHWNSTLKRKF SI EKK
Sbjct: 61  GVERCAFTAEEDEIIVEAHGKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFSSITEKK 120

Query: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGS-ELWPVTQQQSISS 180
           TDC SQGST+SV NQCLSPS SETS SI S++MEPST+LTL LPG+ +L PV +QQSISS
Sbjct: 121 TDCTSQGSTVSVLNQCLSPSESETSDSIPSTEMEPSTRLTLCLPGTDQLSPVRRQQSISS 180

Query: 181 SPVKSEEKQEGKRTCFREELLCVMQKMIKEE 211
              + EE++  KR CF EE + VMQKMIKEE
Sbjct: 181 ED-RMEERERQKRACFGEEFMSVMQKMIKEE 210

BLAST of Carg19667 vs. ExPASy Swiss-Prot
Match: O23160 (Transcription factor MYB73 OS=Arabidopsis thaliana OX=3702 GN=MYB73 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 9.5e-45
Identity = 116/265 (43.77%), Postives = 147/265 (55.47%), Query Frame = 0

Query: 8   IDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAF 67
           ++R+KGPWSPEED++L+ LVQ  G RNWS+IS+SI GRSGKSCRLRW NQL P VE RAF
Sbjct: 9   MERIKGPWSPEEDDLLQRLVQKHGPRNWSLISKSIPGRSGKSCRLRWCNQLSPEVEHRAF 68

Query: 68  TAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKKTD----- 127
           + EEDE I  AHA++GNKWATI+RLLNGRTDNAIKNHWNSTLKRK CS+  +  D     
Sbjct: 69  SQEEDETIIRAHARFGNKWATISRLLNGRTDNAIKNHWNSTLKRK-CSVEGQSCDFGGNG 128

Query: 128 --------------CASQGSTISVSNQCL--SPSGSETSH-----------------SIR 187
                          AS G  +S        SPSGS+ S                  +  
Sbjct: 129 GYDGNLGEEQPLKRTASGGGGVSTGLYMSPGSPSGSDVSEQSSGGAHVFKPTVRSEVTAS 188

Query: 188 SSDMEPSTQLTLSLP------------------------GSELWPVTQQQSISSSPVKSE 211
           SS  +P T L+LSLP                         +EL+PV +++ +       E
Sbjct: 189 SSGEDPPTYLSLSLPWTDETVRVNEPVQLNQNTVMDGGYTAELFPVRKEEQVE----VEE 248

BLAST of Carg19667 vs. ExPASy Swiss-Prot
Match: Q9FDW1 (Transcription factor MYB44 OS=Arabidopsis thaliana OX=3702 GN=MYB44 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 5.2e-43
Identity = 101/186 (54.30%), Postives = 120/186 (64.52%), Query Frame = 0

Query: 9   DRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAFT 68
           DR+KGPWSPEEDE LR LV   G RNW++IS+SI GRSGKSCRLRW NQL P VE R F+
Sbjct: 3   DRIKGPWSPEEDEQLRRLVVKYGPRNWTVISKSIPGRSGKSCRLRWCNQLSPQVEHRPFS 62

Query: 69  AEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKKTD------ 128
           AEEDE I  AHA++GNKWATIARLLNGRTDNA+KNHWNSTLKRK      +  D      
Sbjct: 63  AEEDETIARAHAQFGNKWATIARLLNGRTDNAVKNHWNSTLKRKCGGYDHRGYDGSEDHR 122

Query: 129 ----CASQGSTISVSNQCLSPSGSETSHSIRSSD---MEPSTQL--TLSLPGSELWPVTQ 180
                 S GS   V+   +SP GS T   +  S    + PS +L   +  PG+ + P+  
Sbjct: 123 PVKRSVSAGSPPVVTGLYMSP-GSPTGSDVSDSSTIPILPSVELFKPVPRPGAVVLPLPI 182

BLAST of Carg19667 vs. ExPASy Swiss-Prot
Match: Q9SN12 (Transcription factor MYB77 OS=Arabidopsis thaliana OX=3702 GN=MYB77 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 6.4e-41
Identity = 115/264 (43.56%), Postives = 138/264 (52.27%), Query Frame = 0

Query: 9   DRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAFT 68
           DRVKGPWS EEDE LR +V+  G RNWS IS+SI GRSGKSCRLRW NQL P VE R F+
Sbjct: 3   DRVKGPWSQEEDEQLRRMVEKYGPRNWSAISKSIPGRSGKSCRLRWCNQLSPEVEHRPFS 62

Query: 69  AEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCS-------IAEKKT 128
            EEDE I  A A++GNKWATIARLLNGRTDNA+KNHWNSTLKRK CS       + E + 
Sbjct: 63  PEEDETIVTARAQFGNKWATIARLLNGRTDNAVKNHWNSTLKRK-CSGGVAVTTVTETEE 122

Query: 129 DCASQGSTISVSNQCL-------------SPSGSETSHS--------------------- 188
           D        SVS                 SP+G + S S                     
Sbjct: 123 DQDRPKKRRSVSFDSAFAPVDTGLYMSPESPNGIDVSDSSTIPSPSSPVAQLFKPMPISG 182

Query: 189 ------------IRSSDMEPSTQLTLSLPGSELWPVTQQQS---------ISSSPVKSEE 211
                       + SS  +P T L+LSLPG+E    +   +          S   +  EE
Sbjct: 183 GFTVVPQPLPVEMSSSSEDPPTSLSLSLPGAENTSSSHNNNNNALMFPRFESQMKINVEE 242

BLAST of Carg19667 vs. ExPASy Swiss-Prot
Match: Q42575 (Transcription factor MYB1 OS=Arabidopsis thaliana OX=3702 GN=MYB1 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 8.6e-38
Identity = 87/173 (50.29%), Postives = 107/173 (61.85%), Query Frame = 0

Query: 3   SRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGV 62
           S +G+ DRVKGPWS EED++L  LV+  GARNWS I++SI GRSGKSCRLRW NQL P +
Sbjct: 46  SGRGRRDRVKGPWSKEEDDVLSELVKRLGARNWSFIARSIPGRSGKSCRLRWCNQLNPNL 105

Query: 63  ERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK-- 122
            R +FT  ED+ I  AHA +GNKWA IA+LL GRTDNAIKNHWNS L+R+F    + K  
Sbjct: 106 IRNSFTEVEDQAIIAAHAIHGNKWAVIAKLLPGRTDNAIKNHWNSALRRRFIDFEKAKNI 165

Query: 123 ---------------TDCASQGSTISVSNQC------LSPSGSETSHSIRSSD 153
                          T  AS   T+S    C      +SP G E + S+  S+
Sbjct: 166 GTGSLVVDDSGFDRTTTVASSEETLSSGGGCHVTTPIVSPEGKEATTSMEMSE 218

BLAST of Carg19667 vs. ExPASy Swiss-Prot
Match: O04192 (Transcription factor MYB25 OS=Arabidopsis thaliana OX=3702 GN=MYB25 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.9e-32
Identity = 66/107 (61.68%), Postives = 81/107 (75.70%), Query Frame = 0

Query: 6   GKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERR 65
           G   +VKGPW PE+DE L  LV+  G RNW++IS+ I GRSGKSCRLRW NQL P ++R+
Sbjct: 44  GGKSKVKGPWLPEQDEALTRLVKMCGPRNWNLISRGIPGRSGKSCRLRWCNQLDPILKRK 103

Query: 66  AFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRK 113
            F+ EE+ +I  A A  GNKW+ IA+LL GRTDNAIKNHWNS L+RK
Sbjct: 104 PFSDEEEHMIMSAQAVLGNKWSVIAKLLPGRTDNAIKNHWNSNLRRK 150

BLAST of Carg19667 vs. ExPASy TrEMBL
Match: A0A6J1E4J2 (transcription factor MYB44-like OS=Cucurbita moschata OX=3662 GN=LOC111430691 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.5e-112
Identity = 206/210 (98.10%), Postives = 208/210 (99.05%), Query Frame = 0

Query: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60
           MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP
Sbjct: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60

Query: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120
           GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK
Sbjct: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120

Query: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180
           TDCASQGSTIS+SNQCLSPSGSETS SIRSSDMEPSTQLTLSLPGSELWPVTQQQ ISSS
Sbjct: 121 TDCASQGSTISISNQCLSPSGSETSDSIRSSDMEPSTQLTLSLPGSELWPVTQQQCISSS 180

Query: 181 PVKSEEKQEGKRTCFREELLCVMQKMIKEE 211
           PVKSEEKQ+GKRTCFREELLCVMQKMIKEE
Sbjct: 181 PVKSEEKQKGKRTCFREELLCVMQKMIKEE 210

BLAST of Carg19667 vs. ExPASy TrEMBL
Match: A0A6J1JCR2 (transcription factor MYB44-like OS=Cucurbita maxima OX=3661 GN=LOC111483237 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 5.7e-109
Identity = 203/210 (96.67%), Postives = 206/210 (98.10%), Query Frame = 0

Query: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60
           MGSRKG IDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP
Sbjct: 1   MGSRKGNIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60

Query: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120
           GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK
Sbjct: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120

Query: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180
           TDCASQGSTISVSNQCLSPSGSETS SIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS
Sbjct: 121 TDCASQGSTISVSNQCLSPSGSETSDSIRSSDMEPSTQLTLSLPGSELWPVTQQQSISSS 180

Query: 181 PVKSEEKQEGKRTCFREELLCVMQKMIKEE 211
           P +SEEKQ+GKRTCF EELLCVMQKM+KEE
Sbjct: 181 P-RSEEKQKGKRTCFGEELLCVMQKMVKEE 209

BLAST of Carg19667 vs. ExPASy TrEMBL
Match: A0A5A7T4X0 (Transcription factor MYB44-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold379G00990 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 9.5e-80
Identity = 159/204 (77.94%), Postives = 172/204 (84.31%), Query Frame = 0

Query: 8   IDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAF 67
           IDRVKGPWSPEED +LRMLVQ+QGARNWS+ISQSIHGRSGKSCRLRWFNQLCPGVERRAF
Sbjct: 8   IDRVKGPWSPEEDLLLRMLVQNQGARNWSLISQSIHGRSGKSCRLRWFNQLCPGVERRAF 67

Query: 68  TAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKKTDCASQG 127
           T EED++I EAH KYGNKWATIARLLNGRTDNAIKNHWNSTLKRKF S  EKKTDC SQG
Sbjct: 68  TPEEDKIIVEAHRKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFSSNGEKKTDCGSQG 127

Query: 128 STISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGS-ELWPVTQQQSISSSPVKSEE 187
           STIS  NQCLS  GSE+S S RS +M+PST+LTL LPGS EL PV  Q+SIS  PV+  E
Sbjct: 128 STISGWNQCLSRCGSESSDSDRSEEMKPSTRLTLCLPGSDELGPVQPQKSISCLPVERNE 187

Query: 188 KQEGKRTCFREELLCVMQKMIKEE 211
           +   K  C  EE +CVMQKMIKEE
Sbjct: 188 RV--KLPCLGEEFMCVMQKMIKEE 209

BLAST of Carg19667 vs. ExPASy TrEMBL
Match: A0A1S4E1N4 (transcription factor MYB44-like OS=Cucumis melo OX=3656 GN=LOC107991462 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 9.5e-80
Identity = 159/204 (77.94%), Postives = 172/204 (84.31%), Query Frame = 0

Query: 8   IDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAF 67
           IDRVKGPWSPEED +LRMLVQ+QGARNWS+ISQSIHGRSGKSCRLRWFNQLCPGVERRAF
Sbjct: 8   IDRVKGPWSPEEDLLLRMLVQNQGARNWSLISQSIHGRSGKSCRLRWFNQLCPGVERRAF 67

Query: 68  TAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKKTDCASQG 127
           T EED++I EAH KYGNKWATIARLLNGRTDNAIKNHWNSTLKRKF S  EKKTDC SQG
Sbjct: 68  TPEEDKIIVEAHRKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFSSNGEKKTDCGSQG 127

Query: 128 STISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGS-ELWPVTQQQSISSSPVKSEE 187
           STIS  NQCLS  GSE+S S RS +M+PST+LTL LPGS EL PV  Q+SIS  PV+  E
Sbjct: 128 STISGWNQCLSRCGSESSDSDRSEEMKPSTRLTLCLPGSDELGPVQPQKSISCLPVERNE 187

Query: 188 KQEGKRTCFREELLCVMQKMIKEE 211
           +   K  C  EE +CVMQKMIKEE
Sbjct: 188 RV--KLPCLGEEFMCVMQKMIKEE 209

BLAST of Carg19667 vs. ExPASy TrEMBL
Match: A0A6J1FV84 (transcription factor MYB44-like OS=Cucurbita moschata OX=3662 GN=LOC111447156 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 9.2e-75
Identity = 153/211 (72.51%), Postives = 171/211 (81.04%), Query Frame = 0

Query: 1   MGSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCP 60
           M S +  IDRVKGPWSPEED++LR+LVQ  GARNWS+I QSIHGRSGKSCRLRWFNQLCP
Sbjct: 1   MTSEEWDIDRVKGPWSPEEDQLLRILVQRYGARNWSVIGQSIHGRSGKSCRLRWFNQLCP 60

Query: 61  GVERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK 120
            VER+AFTAEEDEVI EAHAK+GNKWATIARLLNGRTDNAIKNHWNSTLKRKF SIAE K
Sbjct: 61  DVERQAFTAEEDEVIVEAHAKHGNKWATIARLLNGRTDNAIKNHWNSTLKRKFSSIAEIK 120

Query: 121 TDCASQGSTISVSNQCLSPSGSETSHSIRSSDMEPSTQLTLSLPGSELWPV-TQQQSISS 180
           T+C S GSTIS+ NQCLSPS S+T+ SI S +  PST+L+LSLPG+E  P   QQQSISS
Sbjct: 121 TECTSHGSTISIGNQCLSPSRSDTNDSIPSQNKRPSTRLSLSLPGTESSPPRPQQQSISS 180

Query: 181 SPVKSEEKQEGKRTCFREELLCVMQKMIKEE 211
            P   E K +  R    +E L VMQ MI+EE
Sbjct: 181 LPEDREAKAKSARC--GKEFLSVMQLMIREE 209

BLAST of Carg19667 vs. TAIR 10
Match: AT2G23290.1 (myb domain protein 70 )

HSP 1 Score: 183.3 bits (464), Expect = 2.3e-46
Identity = 119/267 (44.57%), Postives = 145/267 (54.31%), Query Frame = 0

Query: 2   GSRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPG 61
           GS + ++DR+KGPWSPEED++L+ LVQ  G RNWS+IS+SI GRSGKSCRLRW NQL P 
Sbjct: 3   GSTRKEMDRIKGPWSPEEDDLLQSLVQKHGPRNWSLISKSIPGRSGKSCRLRWCNQLSPE 62

Query: 62  VERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCS------ 121
           VE R FTAEED+ I  AHA++GNKWATIARLLNGRTDNAIKNHWNSTLKRK CS      
Sbjct: 63  VEHRGFTAEEDDTIILAHARFGNKWATIARLLNGRTDNAIKNHWNSTLKRK-CSGGGGGG 122

Query: 122 ------------------IAEKKTDCASQGSTISVSNQCLSPSGSETSHSIRSS------ 181
                               EK     + G    V    LSP+GS+ S   +SS      
Sbjct: 123 EEGQSCDFGGNGGYDGNLTDEKPLKRRASGGGGVVVVTALSPTGSDVSEQSQSSGSVLPV 182

Query: 182 --------------------------DMEPSTQLTLSLPGSELWPVTQQQSISSSPVKSE 211
                                     + +P T L LSLP    W           PVK E
Sbjct: 183 SSSCHVFKPTARAGGVVIESSSPEEEEKDPMTCLRLSLP----WVNESTTPPELFPVKRE 242

BLAST of Carg19667 vs. TAIR 10
Match: AT4G37260.1 (myb domain protein 73 )

HSP 1 Score: 181.8 bits (460), Expect = 6.8e-46
Identity = 116/265 (43.77%), Postives = 147/265 (55.47%), Query Frame = 0

Query: 8   IDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAF 67
           ++R+KGPWSPEED++L+ LVQ  G RNWS+IS+SI GRSGKSCRLRW NQL P VE RAF
Sbjct: 9   MERIKGPWSPEEDDLLQRLVQKHGPRNWSLISKSIPGRSGKSCRLRWCNQLSPEVEHRAF 68

Query: 68  TAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKKTD----- 127
           + EEDE I  AHA++GNKWATI+RLLNGRTDNAIKNHWNSTLKRK CS+  +  D     
Sbjct: 69  SQEEDETIIRAHARFGNKWATISRLLNGRTDNAIKNHWNSTLKRK-CSVEGQSCDFGGNG 128

Query: 128 --------------CASQGSTISVSNQCL--SPSGSETSH-----------------SIR 187
                          AS G  +S        SPSGS+ S                  +  
Sbjct: 129 GYDGNLGEEQPLKRTASGGGGVSTGLYMSPGSPSGSDVSEQSSGGAHVFKPTVRSEVTAS 188

Query: 188 SSDMEPSTQLTLSLP------------------------GSELWPVTQQQSISSSPVKSE 211
           SS  +P T L+LSLP                         +EL+PV +++ +       E
Sbjct: 189 SSGEDPPTYLSLSLPWTDETVRVNEPVQLNQNTVMDGGYTAELFPVRKEEQVE----VEE 248

BLAST of Carg19667 vs. TAIR 10
Match: AT5G67300.1 (myb domain protein r1 )

HSP 1 Score: 176.0 bits (445), Expect = 3.7e-44
Identity = 101/186 (54.30%), Postives = 120/186 (64.52%), Query Frame = 0

Query: 9   DRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAFT 68
           DR+KGPWSPEEDE LR LV   G RNW++IS+SI GRSGKSCRLRW NQL P VE R F+
Sbjct: 3   DRIKGPWSPEEDEQLRRLVVKYGPRNWTVISKSIPGRSGKSCRLRWCNQLSPQVEHRPFS 62

Query: 69  AEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKKTD------ 128
           AEEDE I  AHA++GNKWATIARLLNGRTDNA+KNHWNSTLKRK      +  D      
Sbjct: 63  AEEDETIARAHAQFGNKWATIARLLNGRTDNAVKNHWNSTLKRKCGGYDHRGYDGSEDHR 122

Query: 129 ----CASQGSTISVSNQCLSPSGSETSHSIRSSD---MEPSTQL--TLSLPGSELWPVTQ 180
                 S GS   V+   +SP GS T   +  S    + PS +L   +  PG+ + P+  
Sbjct: 123 PVKRSVSAGSPPVVTGLYMSP-GSPTGSDVSDSSTIPILPSVELFKPVPRPGAVVLPLPI 182

BLAST of Carg19667 vs. TAIR 10
Match: AT3G50060.1 (myb domain protein 77 )

HSP 1 Score: 169.1 bits (427), Expect = 4.5e-42
Identity = 115/264 (43.56%), Postives = 138/264 (52.27%), Query Frame = 0

Query: 9   DRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGVERRAFT 68
           DRVKGPWS EEDE LR +V+  G RNWS IS+SI GRSGKSCRLRW NQL P VE R F+
Sbjct: 3   DRVKGPWSQEEDEQLRRMVEKYGPRNWSAISKSIPGRSGKSCRLRWCNQLSPEVEHRPFS 62

Query: 69  AEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCS-------IAEKKT 128
            EEDE I  A A++GNKWATIARLLNGRTDNA+KNHWNSTLKRK CS       + E + 
Sbjct: 63  PEEDETIVTARAQFGNKWATIARLLNGRTDNAVKNHWNSTLKRK-CSGGVAVTTVTETEE 122

Query: 129 DCASQGSTISVSNQCL-------------SPSGSETSHS--------------------- 188
           D        SVS                 SP+G + S S                     
Sbjct: 123 DQDRPKKRRSVSFDSAFAPVDTGLYMSPESPNGIDVSDSSTIPSPSSPVAQLFKPMPISG 182

Query: 189 ------------IRSSDMEPSTQLTLSLPGSELWPVTQQQS---------ISSSPVKSEE 211
                       + SS  +P T L+LSLPG+E    +   +          S   +  EE
Sbjct: 183 GFTVVPQPLPVEMSSSSEDPPTSLSLSLPGAENTSSSHNNNNNALMFPRFESQMKINVEE 242

BLAST of Carg19667 vs. TAIR 10
Match: AT3G09230.1 (myb domain protein 1 )

HSP 1 Score: 158.7 bits (400), Expect = 6.1e-39
Identity = 87/173 (50.29%), Postives = 107/173 (61.85%), Query Frame = 0

Query: 3   SRKGKIDRVKGPWSPEEDEMLRMLVQSQGARNWSIISQSIHGRSGKSCRLRWFNQLCPGV 62
           S +G+ DRVKGPWS EED++L  LV+  GARNWS I++SI GRSGKSCRLRW NQL P +
Sbjct: 46  SGRGRRDRVKGPWSKEEDDVLSELVKRLGARNWSFIARSIPGRSGKSCRLRWCNQLNPNL 105

Query: 63  ERRAFTAEEDEVITEAHAKYGNKWATIARLLNGRTDNAIKNHWNSTLKRKFCSIAEKK-- 122
            R +FT  ED+ I  AHA +GNKWA IA+LL GRTDNAIKNHWNS L+R+F    + K  
Sbjct: 106 IRNSFTEVEDQAIIAAHAIHGNKWAVIAKLLPGRTDNAIKNHWNSALRRRFIDFEKAKNI 165

Query: 123 ---------------TDCASQGSTISVSNQC------LSPSGSETSHSIRSSD 153
                          T  AS   T+S    C      +SP G E + S+  S+
Sbjct: 166 GTGSLVVDDSGFDRTTTVASSEETLSSGGGCHVTTPIVSPEGKEATTSMEMSE 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6576937.11.5e-140100.00Transcription factor MYB44, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_022922824.11.1e-11198.10transcription factor MYB44-like [Cucurbita moschata][more]
XP_023552561.11.1e-10996.67transcription factor MYB73-like [Cucurbita pepo subsp. pepo][more]
XP_022985148.11.2e-10896.67transcription factor MYB44-like [Cucurbita maxima][more]
XP_038900077.12.6e-8478.20transcription factor MYB73-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O231609.5e-4543.77Transcription factor MYB73 OS=Arabidopsis thaliana OX=3702 GN=MYB73 PE=1 SV=1[more]
Q9FDW15.2e-4354.30Transcription factor MYB44 OS=Arabidopsis thaliana OX=3702 GN=MYB44 PE=1 SV=1[more]
Q9SN126.4e-4143.56Transcription factor MYB77 OS=Arabidopsis thaliana OX=3702 GN=MYB77 PE=1 SV=1[more]
Q425758.6e-3850.29Transcription factor MYB1 OS=Arabidopsis thaliana OX=3702 GN=MYB1 PE=2 SV=1[more]
O041921.9e-3261.68Transcription factor MYB25 OS=Arabidopsis thaliana OX=3702 GN=MYB25 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E4J25.5e-11298.10transcription factor MYB44-like OS=Cucurbita moschata OX=3662 GN=LOC111430691 PE... [more]
A0A6J1JCR25.7e-10996.67transcription factor MYB44-like OS=Cucurbita maxima OX=3661 GN=LOC111483237 PE=4... [more]
A0A5A7T4X09.5e-8077.94Transcription factor MYB44-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S4E1N49.5e-8077.94transcription factor MYB44-like OS=Cucumis melo OX=3656 GN=LOC107991462 PE=4 SV=... [more]
A0A6J1FV849.2e-7572.51transcription factor MYB44-like OS=Cucurbita moschata OX=3662 GN=LOC111447156 PE... [more]
Match NameE-valueIdentityDescription
AT2G23290.12.3e-4644.57myb domain protein 70 [more]
AT4G37260.16.8e-4643.77myb domain protein 73 [more]
AT5G67300.13.7e-4454.30myb domain protein r1 [more]
AT3G50060.14.5e-4243.56myb domain protein 77 [more]
AT3G09230.16.1e-3950.29myb domain protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001005SANT/Myb domainSMARTSM00717santcoord: 11..60
e-value: 8.8E-11
score: 51.8
coord: 63..111
e-value: 1.6E-15
score: 67.5
IPR001005SANT/Myb domainPROSITEPS50090MYB_LIKEcoord: 7..58
score: 11.393172
IPR001005SANT/Myb domainPROSITEPS50090MYB_LIKEcoord: 59..109
score: 9.442289
IPR001005SANT/Myb domainCDDcd00167SANTcoord: 66..109
e-value: 4.14607E-11
score: 54.5038
IPR001005SANT/Myb domainCDDcd00167SANTcoord: 14..56
e-value: 4.31832E-11
score: 54.5038
NoneNo IPR availableGENE3D1.10.10.60coord: 66..112
e-value: 3.9E-21
score: 76.7
NoneNo IPR availableGENE3D1.10.10.60coord: 11..63
e-value: 3.5E-21
score: 76.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..159
NoneNo IPR availablePANTHERPTHR45614:SF145TRANSCRIPTION FACTOR MYB44-LIKEcoord: 5..212
NoneNo IPR availablePANTHERPTHR45614MYB PROTEIN-RELATEDcoord: 5..212
IPR017930Myb domainPFAMPF00249Myb_DNA-bindingcoord: 12..57
e-value: 1.4E-14
score: 54.0
coord: 64..107
e-value: 4.3E-15
score: 55.6
IPR017930Myb domainPROSITEPS51294HTH_MYBcoord: 60..113
score: 23.134005
IPR017930Myb domainPROSITEPS51294HTH_MYBcoord: 7..58
score: 18.558674
IPR009057Homeobox-like domain superfamilySUPERFAMILY46689Homeodomain-likecoord: 9..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19667-RACarg19667-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding